CN112022730A

CN112022730A - Agent combination for skin care

Info

Publication number: CN112022730A
Application number: CN201910397394.1A
Authority: CN
Inventors: 谌智鑫
Original assignee: Xi'an Bohong Biotechnology Co ltd
Current assignee: Xi'an Bohong Biotechnology Co ltd
Priority date: 2019-05-14
Filing date: 2019-05-14
Publication date: 2020-12-04

Abstract

The invention provides an agent combination for skin care, which comprises a first agent, a second agent and a third agent, wherein the first agent comprises glycerin, butanediol and sodium hyaluronate; the second agent comprises tranexamic acid, ascorbic acid and dipotassium glycyrrhizinate; the third agent comprises an extract of exosomes of umbilical cord mesenchymal stem cells. The skin care agent composition has better wrinkle removing and antiallergic effects and better absorbability, and can better improve the skin state of a person with wrinkles or a person with skin allergy after the skin care agent composition is used.

Description

Agent combination for skin care

Technical Field

The invention relates to the field of skin care products, in particular to a skin care reagent composition and application thereof.

Background

With the continuous improvement of living standard of people, people are more and more demanding on beauty, especially on mild and safe skin care products, because the factors of air pollution, ultraviolet ray damage, environmental allergen invasion, unsafe skin care products and the like increase the damage to human skin, and the phenomena of face dryness, discomfort, allergy and the like can be caused after a long time.

With the increasing of age and working pressure, the radiation of electronic products is increased and the surrounding environment is polluted, after a certain age, the grease and collagen of skin and skin are lost, and the aging is gradually aggravated, so that the problems of fine lines, looseness, dark yellow and pigmentation and the like can occur on the face. Therefore, pursuing beauty becomes a permanent theme for the public.

Various skin care products are available on the market at present, but basically, the skin care products remind users of trying to determine whether allergy exists or not and then apply the skin care products on the face, so that people with skin allergy or skin diseases cannot directly use the skin care products. Because these skin care products have certain irritation to the skin during the use process, can cause skin allergy, not only can not play the role of skin care, but also can have opposite side effects. Therefore, for people with skin allergy, it is a difficult task to buy a suitable skin care product.

Patent CN108354865A discloses a moisturizing, wrinkle-removing and antiallergic composition, which comprises the following components in percentage by weight: 0.002-0.5% of bioactive polypeptide, 5-15% of skin conditioner, 10-30% of matrix component and the balance of cold spring water. The cold spring water collected from the volcano of the Wudalianchi is added in the patent, is rich in trace elements with the proportion equivalent to the proportion required by a human body, can effectively regulate the digestive function and the water and salt metabolism of the human body, can improve the functions of a biological enzyme system and an endocrine system of the body, has good medicinal value and has good curative effect on skin diseases; bioactive polypeptide and trace elements in the cold spring water of the Wudalianchi are used in a compounding way, polypeptide molecules and the trace elements in the ionic form are combined and permeate into epidermal cells, and the combined polypeptide molecules and the trace elements are delivered to skin tissue structures through blood microcirculation to activate the cells of the skin tissue structures, so that the composition has the effects of moisturizing, anti-wrinkle, anti-allergy, anti-inflammation and spot lightening; not only is suitable for general people, but also can further improve skin diseases; however, the patent needs to rely on components collected from specific regions, such as cold spring water collected from volcano of Wudalianchi, and the practicability is not strong; and the polypeptide has large molecular weight, is not easy to be absorbed by skin and influences the skin care effect.

Therefore, there is a need for a skin care product with better wrinkle-removing and antiallergic effects and better absorption properties, which can improve the skin condition of people with wrinkles or people with skin allergy.

Disclosure of Invention

In view of the above, the present invention aims to provide a skin care product with better wrinkle removing and antiallergic effects and better absorbability, so that the skin condition of a person with wrinkles or a person with skin allergy can be better improved after the skin care product is used.

Therefore, the invention provides the following technical scheme.

In a first aspect, the present invention provides an agent combination for skin care comprising a first agent, a second agent and a third agent, wherein,

the first agent comprises glycerol, butylene glycol, and sodium hyaluronate;

the second agent comprises tranexamic acid, ascorbic acid and dipotassium glycyrrhizinate;

the third agent comprises an extract of exosomes of umbilical cord mesenchymal stem cells.

In a preferred embodiment, wherein, in parts by weight,

the first agent comprises 1-6 parts of glycerol, 1-6 parts of butanediol and 0.1-1.5 parts of sodium hyaluronate. For example, the glycerol may be present in 1-5, 1-4, 1-3, 1-2, 2-6, 3-6, 4-6, or 5-6 parts by weight; for example, the butanediol can be present in a weight fraction of 1 to 5, 1 to 4, 1 to 3, 1 to 2, 2 to 6, 3 to 6, 4 to 6, or 5 to 6; for example, the sodium hyaluronate may be present in an amount of 0.1-1.4, 0.1-1.3, 0.1-1.2, 0.1-1.1, 0.1-1, 0.1-0.9, 0.1-0.8, 0.1-0.7, 0.1-0.6, 0.1-0.5, 0.1-0.4, 0.1-0.3, 0.2-1.5, 0.3-1.5, 0.4-1.5, 0.5-1.5, 0.6-1.5, 0.7-1.5, 0.8-1.5, 0.9-1.5, 1-1.5, 1.1-1.5, 1.2-1.5, or 1.3-1.5 by weight.

In a preferred embodiment, wherein, in parts by weight,

the second reagent comprises 1-4 parts of tranexamic acid, 1-4 parts of ascorbic acid and 0.1-1 part of dipotassium glycyrrhizinate. For example, the tranexamic acid may be present in a weight fraction of 1-3.5, 1-3, 1-2.5, 1-2, 1-1.5, 2-4, 2.5-4, 3-4, or 3.5-4; the ascorbic acid can be 1-3.5, 1-3, 1-2.5, 1-2, 1-1.5, 2-4, 2.5-4, 3-4 or 3.5-4 by weight; the weight portion of the dipotassium glycyrrhizinate can be 0.1-0.9, 0.1-0.8, 0.1-0.7, 0.1-0.6, 0.1-0.5, 0.1-0.4, 0.1-0.3, 0.2-1, 0.3-1, 0.4-1, 0.5-1, 0.6-1, 0.7-1, 0.8-1 or 0.9-1.

In a preferred embodiment, wherein, in parts by weight,

the third reagent comprises 0.1-1 part of umbilical cord mesenchymal stem cell exosome extract. For example, the weight part of the umbilical cord mesenchymal stem cell exosome extract may be 0.1-0.9, 0.1-0.8, 0.1-0.7, 0.1-0.6, 0.1-0.5, 0.1-0.4, 0.1-0.3, 0.2-1, 0.3-1, 0.4-1, 0.5-1, 0.6-1, 0.7-1, 0.8-1 or 0.9-1.

In a second aspect, the present invention provides an agent combination for skin care comprising a first agent, a second agent and a third agent, wherein the first agent, the second agent and the third agent are, in parts by weight,

the first reagent comprises 1-6 parts of glycerol, 1-6 parts of butanediol and 0.1-1.5 parts of sodium hyaluronate;

the second reagent comprises 1-4 parts of tranexamic acid, 1-4 parts of ascorbic acid and 0.1-1 part of dipotassium glycyrrhizinate;

the third reagent comprises 0.1-1 part of umbilical cord mesenchymal stem cell exosome extract.

In a preferred embodiment, the first agent further comprises one or more of a thickening agent, an emulsifier, a surfactant, a skin conditioning agent, a grease, a fragrance, and a preservative.

In a more preferred embodiment, the thickening agent may be selected from one or more of carbomer, xanthan gum, sodium carrageenan, acacia, polyethylene glycol-14M, polyethylene glycol-90M, succinoglycan, SEPIPLUS 265, hydroxyethyl cellulose, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose.

In a more preferred embodiment, the emulsifier may be selected from the group consisting of cetearyl olivate, sorbitan olivate, polysorbate-60, polysorbate-80, polysorbate-85, methylgluco-sesquistearate, PEG-20 methylgluco-sesquistearate, PEG-40 hydrogenated castor oil, PPG-26-buteth-26, PEG-4 polyglyceryl-2 stearate, PEG-60 hydrogenated castor oil, steareth-2, steareth-21, PPG-13-decyltetraeth-24, cetearyl glucoside, PEG-100 stearate, ceteth-20, glyceryl stearate SE, cocoyl glucoside, ceteth-25, PEG-40 stearate, glyceryl stearate, One or more of polyglyceryl-3 methylglucdistearate, glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-10 dioleate, polyglyceryl-10 laurate, polyglyceryl-10 isostearate, polyglyceryl-10 oleate, polyglyceryl-10 diisostearate, polyglyceryl-6 laurate, polyglyceryl-6 myristate, sucrose distearate and sucrose polystearate.

In a more preferred embodiment, the surfactant may be selected from one or more of Tween, SPAN, triton, EMULGEN series surfactants, macrogolglycerides laurate, anionic alkylpolyglycosides, polyglycerol-3 methylglucdistearate, cetearyl alcohol and cetearyl glucoside, dodecyl dimethyl betaine, 3 armet 1, SHO62C and AM PHITOL.

In a more preferred embodiment, the skin conditioning agent may be selected from the group consisting of kojic acid, ascorbic acid, ascorbyl glucoside, sodium hyaluronate, arbutin, tranexamic acid, niacinamide, phytosterols, phenethyl resorcinol, turmeric root extract, birch bark extract, ceramide 2, ceramide 3, panthenol, acetyl phytosphingosine, dipotassium glycyrrhizinate, carboxymethyl chitosan, trehalose, glycerol, hydroxyethyl urea, propylene glycol, beta-glucan, glyceryl polyether-26, allantoin, ethanol, resveratrol, Pterocarpus marsupium bark extract, coleus forskohlii root extract, pepper seed extract, glyceryl polyacrylate, warm water, Tremella entity extract, ubiquinone, cholesterol stearate, ascorbyl dipalmitate, tocopherol, tocopheryl acetate, bisabolol, ascorbyl tetraisopalmitate palmitate, tocopherol, One or more of pyridoxine dicaprylate, pyridoxine dipalmitate, retinol palmitate, and the phytosterol macadamia oleate.

In a more preferred embodiment, the oil or fat may be selected from olive oil, macadamia nut oil, sweet almond oil, mineral oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, meadowfoam seed oil, safflower seed oil, rosa canina oil, anise oil, argan oil, jojoba seed oil, sunflower seed oil, oil of seradipalm, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated polyisobutene, isohexadecane, jojoba esters, polydimethylsiloxane, diisostearyl malate, isododecane, diethylhexyl carbonate, dioctyl carbonate, isopropyl lauroyl sarcosinate, isononyl isononanoate, hydrogenated polydecene, cetyl ethyl hexanoate, bis-diethoxydiol cyclohexane 1, 4-dicarboxylate, caprylic/capric triglyceride, oleyl erucate, Octyldodecanol myristate, octyldodecanol, dimethicone, octylmethicone, cetyl dimethicone, cetearyl ethylhexanoate, avocado sterol, cyclopentadimethicone, cetyl alcohol, stearyl alcohol, cetearyl alcohol, behenyl alcohol, batyl alcohol, lauric acid, shea butter, myristic acid, palmitic acid, stearic acid, beeswax, candelilla wax, carnauba wax, lanolin, ozokerite, jojoba seed wax, paraffin, petrolatum, microcrystalline wax, hydrogenated rice bran wax, hydrogenated cocoglycerides, myristyl myristate, di-diglycerol polyacyladipate-2, shea butter, and mugwort palm seed fat.

In a more preferred embodiment, the fragrance may be selected from one or more of sage oil, chamomile oil, clove oil, balsam oil, peppermint oil, cinnamon leaf oil, basswood flower oil, juniper berry oil, vetiver oil, frankincense oil, Persian resin oil, labdanum oil and lavandin oil.

In a more preferred embodiment, the preservative may be selected from one or more of methylparaben, propylparaben, DMDM hydantoin, phenoxyethanol, glyceryl caprylate, ethylhexyl glycerin, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, caprylhydroxamic acid, and glyceryl caprylate.

In a preferred embodiment, the second agent further comprises one or more of a thickening agent, an emulsifier, a surfactant, a skin conditioning agent, a grease, a fragrance, and a preservative.

In a more preferred embodiment, the thickening agent may be selected from one or more of carbomer, xanthan gum, sodium carrageenan, acacia, polyethylene glycol-14M, polyethylene glycol-90M, succinoglycan, SEPIPLUS 265, hydroxyethyl cellulose, hydroxypropyl cellulose, and hydroxypropyl methyl cellulose;

In a more preferred embodiment, the skin conditioning agent may be selected from the group consisting of kojic acid, ascorbic acid, ascorbyl glucoside, sodium hyaluronate, arbutin, tranexamic acid, niacinamide, phytosterols, phytosterol/behenyl alcohol/octyldecanol lauroyl glutamate, phenethylresorcinol, turmeric root extract, birch bark extract, ceramide 2, ceramide 3, panthenol, acetylphytosphingosine, dipotassium glycyrrhizinate, carboxymethyl chitosan, trehalose, glycerol, hydroxyethyl urea, propylene glycol, beta-glucan, glyceryl polyether-26, allantoin, ethanol, resveratrol, Pterocarpus marsupium bark extract, Coleus forskohlii root extract, pepper seed extract, glyceryl polyacrylate, warm spring water, Tremella entity extract, ubiquinone, cholesterol stearate, ascorbyl dipalmitate, One or more of tocopherol, tocopheryl acetate, bisabolol, ascorbyl tetraisopalmitate, pyridoxine dioctanoate, pyridoxine dipalmitate, retinol palmitate and the phytosterol macadamia nut oleate.

In preferred embodiments, the third agent further comprises one or more of a thickening agent, an emulsifier, a surfactant, a skin conditioning agent, a grease, a fragrance, and a preservative.

In a preferred embodiment, the first, second and/or third reagent may further comprise water.

In a third aspect, a skin care product is provided, comprising the combination of agents of the present invention, wherein the skin care product can be a lotion, an emulsion, a cream, or a mask.

In a fourth aspect, there is provided a pharmaceutical composition comprising a safe and effective amount of a combination of agents according to the invention.

In addition, the invention also provides the application of the reagent combination in preparing skin care products.

And the use of the combination of agents of the invention for the preparation of a pharmaceutical composition.

Compared with the prior art, the invention has the beneficial effects that:

the umbilical cord mesenchymal stem cells are adult stem cells with self-renewal, proliferation and multidirectional differentiation potential, the source of the umbilical cord mesenchymal stem cells is not limited by ethics, the umbilical cord mesenchymal stem cells are easy to obtain, the proliferation speed is high, and the immunogenicity is low. The secreted exosomes are one of the important mediators of intercellular regulation.

According to the reagent combination for skin care, the umbilical cord mesenchymal stem cell exosome used in the reagent combination for skin care can simulate partial functions of umbilical cord mesenchymal stem cells to play roles of promoting tissue repair and immunoregulation, the umbilical cord mesenchymal stem cell exosome has no cell nucleus structure and cannot be amplified in a host body, the clinical application has high safety, the application prospect is extremely wide, and the molecular weight of the umbilical cord mesenchymal stem cell exosome is small, so that the skin absorbability is better.

The skin care agent composition provided by the invention contains substances which can better increase skin moisture or reduce skin percutaneous water loss by specific butanediol and sodium hyaluronate.

The specific tranexamic acid, the ascorbic acid and the dipotassium glycyrrhizinate contained in the agent combination can better improve dry or damaged skin, and the agent combination can better resist allergy, diminish inflammation and relieve skin by matching with other specific components in the agent combination.

On the other hand, by screening the types of the components in the skin-care reagent composition and adjusting the weight parts of the components in the skin-care reagent composition, the skin-care reagent composition has the technical effects of better wrinkle removal, better anti-allergy property and better absorbability.

Detailed Description

The present invention will be described in detail below with reference to specific embodiments and examples, and the advantages and various effects of the present invention will be more clearly apparent therefrom. It will be understood by those skilled in the art that these specific embodiments and examples are for the purpose of illustrating the invention and are not to be construed as limiting the invention.

Throughout the specification, unless otherwise specifically noted, terms used herein should be understood as having meanings as commonly used in the art. Thus, unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. If there is a conflict, the present specification will control.

Umbilical cord mesenchymal stem cell exosome

Umbilical cord mesenchymal stem cells are a class of adult stem cells with self-renewal, proliferation and multipotentiality. Because the source of the umbilical cord mesenchymal stem cells is not limited by ethics, the umbilical cord mesenchymal stem cells are easy to obtain, have high proliferation speed and low immunogenicity, are also the main reason for selecting the umbilical cord mesenchymal stem cells, and are widely applied to clinical researches for treating ischemic diseases, cardiovascular diseases, diabetes, tumors, autoimmune diseases and the like at present.

The biological functions of the umbilical cord mesenchymal stem cells are mainly realized by intercellular interaction, secretion of cytokines and up-regulation of inhibitory molecules on cell surfaces, and secreted exosomes are one of the important mediators of intercellular regulation. Exosomes, when initially discovered, are generally considered as "garbage" of cells metabolized during development, and with the continuing advances in biotechnology, researchers have discovered exosomes as a vehicle for cells to exert biological activity. The umbilical cord mesenchymal stem cell exosome can simulate partial functions of umbilical cord mesenchymal stem cells to play the roles of promoting tissue repair and immunoregulation, has no cell nucleus structure, cannot be amplified in a host body, has higher safety in clinical application, can replace skin transplantation to be used for wound healing formed by burns and the like in the future, has extremely wide application prospect, has small molecular weight of exosome, is applied to the field of skin care products, and ensures that the skin absorptivity is better.

The preparation method of the umbilical cord mesenchymal stem cell exosome extract comprises the following steps:

step 1, culturing umbilical cord mesenchymal stem cells

Culturing the umbilical cord mesenchymal stem cells with an alpha-MEM culture medium until the umbilical cord mesenchymal stem cells grow to a fusion degree of more than 80%, collecting a culture solution, washing with PBS for three times, digesting with 0.25% pancreatin for passage, continuing to subculture, collecting a culture supernatant each time, replacing a fresh culture medium, and collecting the culture supernatant in the continuous culture process.

Step 2, extracting exosomes

a. Centrifuging the cell culture supernatant at 400g of 300-;

b. taking the supernatant collected in the step a, centrifuging for 20-30min at 3000g of 2000-3000g, continuously collecting the supernatant, and discarding the precipitate;

c. filtering the supernatant collected in the step b by using a filter with the pore diameter of 0.22 mu m, and collecting filtrate;

d. ultrafiltering the supernatant with a Stired Cell 8050 type ultrafiltration device, wherein the pore diameter of the filter membrane is 30-60nm, adding PBS and ultrafiltering again after ultrafiltration is completed, and repeating for 3 times;

e. washing exosomes on the filter membrane by PBS to prepare a concentrated culture solution, measuring the protein concentration by using a BCA kit, and refrigerating and storing.

Thickening agent

Thickeners can have a significant effect on the appearance of the product. The thickener enables the system to achieve the thickening purpose through a rod-shaped micelle formed by the thickener and a surfactant, a three-dimensional hydration network structure formed by the action of the thickener and water, a self macromolecular long-chain structure and the like.

For the kind of the thickener, the present invention is selected from one or more of carbomer, xanthan gum, sodium carrageenan, acacia, polyethylene glycol-14M, polyethylene glycol-90M, succinoglycan, SEPIPLUS 265, hydroxyethyl cellulose, hydroxypropyl cellulose and hydroxypropyl methyl cellulose.

In a preferred embodiment, for the class of thickeners, the present invention is selected from one or more of xanthan gum, carbomer and hydroxyethyl cellulose.

The amount of the thickener used can be selected by those skilled in the art according to the type of thickener, for example, 0.5 parts by weight or less in the case of xanthan gum, 0.5 parts by weight or less in the case of carbomer, and 0.3 parts by weight or less in the case of hydroxyethylcellulose.

Emulsifier

In the present invention, the emulsifier is a substance capable of improving the surface tension between various constituent phases in an emulsion to form a uniform and stable dispersion or emulsion. Emulsifiers are surface-active substances which have both hydrophilic and lipophilic groups in the molecule and which accumulate at the oil/water interface and reduce the interfacial tension and the energy required to form an emulsion, thereby increasing the energy of the emulsion.

With respect to the type of emulsifier, the present invention is selected from the group consisting of cetearyl olivate, sorbitan olivate, polysorbate-60, polysorbate-80, polysorbate-85, methylgluco-sesquistearate, PEG-20 methylgluco-sesquistearate, PEG-40 hydrogenated castor oil, PPG-26-buteth-26, PEG-4 polyglyceryl-2 stearate, PEG-60 hydrogenated castor oil, steareth-2, steareth-21, PPG-13-decyltetraeth-24, cetearyl glucoside, PEG-100 stearate, ceteth-20, glyceryl stearate SE, cocoyl glucoside, ceteareth-25, PEG-40 stearate, sodium lauryl, One or more of polyglyceryl-3 methylglucdistearate, glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-10 dioleate, polyglyceryl-10 laurate, polyglyceryl-10 isostearate, polyglyceryl-10 oleate, polyglyceryl-10 diisostearate, polyglyceryl-6 laurate, polyglyceryl-6 myristate, sucrose distearate and sucrose polystearate.

In a preferred embodiment, the present invention is selected from one or more of cetearyl olivate, cetearyl glucoside, polyglyceryl-10 oleate and sucrose distearate as to the kind of emulsifier.

As the amount of the emulsifier to be used, those skilled in the art can select the amount according to the kind of the emulsifier.

Surface active agent

In the invention, the surfactant is a substance which is added with a small amount and can cause the interface state of a solution system to change obviously; it has fixed hydrophilic and lipophilic groups and can be directionally arranged on the surface of the solution.

As to the kind of the surfactant, the present invention may be selected from one or more of Tween, SPAN, Triton, EMULGEN series surfactants, macrogolglycerides of lauric acid, anionic alkylpolyglycosides, polyglyceryl-3-methylglucdistearate, cetearyl alcohol and cetearyl glucoside, dodecyl dimethyl betaine, 3ARAMT1, SHO62C and AM PHITOL.

In a preferred embodiment, for the kind of surfactant, the present invention is selected from one or more of polyglyceryl-3-methylglucdistearate, cetearyl alcohol and cetearyl glucoside, and the Tween series.

The amount of the surfactant used can be selected by those skilled in the art depending on the kind of the surfactant, and for example, 0.1 to 1 part by weight can be used when polyglycerin-3-methylglucidistearate is used, and 1 to 3 parts by weight can be used when cetearyl alcohol and cetearyl glucoside are used.

Skin conditioning agent

In the present invention, the skin conditioner means an agent capable of improving the appearance of dry/damaged skin, reducing peeling and restoring skin softness, and also having anti-irritation, anti-inflammatory and skin-soothing effects.

For the class of skin conditioning agents, the present invention is selected from the group consisting of kojic acid, ascorbic acid, ascorbyl glucoside, sodium hyaluronate, arbutin, tranexamic acid, niacinamide, phytosterols, phenylethyl resorcinol, turmeric root extract, birch bark extract, ceramide 2, ceramide 3, panthenol, acetyl phytosphingosine, dipotassium glycyrrhizinate, carboxymethyl chitosan, trehalose, glycerol, hydroxyethyl urea, propylene glycol, beta-glucan, glyceryl polyether-26, allantoin, ethanol, resveratrol, Pterocarpus marsupium bark extract, coleus forskohlii root extract, pepper seed extract, glyceryl polyacrylate, wintergreen, Tremella entity extract, ubiquinone, cholesterol stearate, ascorbyl dipalmitate, tocopherol, tocopheryl acetate, bisabolol, ascorbyl tetraisopalmitate, palmitate, and mixtures thereof, One or more of pyridoxine dicaprylate, pyridoxine dipalmitate, retinol palmitate, and the phytosterol macadamia oleate.

In a preferred embodiment, for the kind of skin conditioning agent, the present invention is selected from one or more of niacinamide, propylene glycol, ubiquinone, cholesterol and sodium hyaluronate.

The amount of the skin conditioning agent to be used is selected by those skilled in the art according to the kind of the skin conditioning agent, and for example, 0.5 to 2 parts by weight may be used when niacinamide is used.

Oil and fat

In the present invention, the oil-and-fat raw materials mainly include oil and wax, which are a generic name of oil-phase raw materials, which are also a base material, and are called oil in a fluid form at normal temperature, and wax in a semisolid form, which are called wax, and the main components thereof are higher fatty acid esters. Mainly has the functions of skin care, lubrication, solidification and shaping and the like.

Depending on the source, oily materials can be broadly classified into animal fats and oils, vegetable fats and oils, mineral fats and oils, semisynthetic fats and oils, and related waxes. Generally, the skin absorbs animal oils best, followed by vegetable oils.

For the type of oil, the oil is selected from olive oil, macadamia nut oil, sweet almond oil, mineral oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, meadowfoam seed oil, safflower seed oil, rosa canina oil, anise oil, argan oil, jojoba seed oil, sunflower seed oil, oil of mazuki, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated polyisobutene, isohexadecane, jojoba esters, polydimethylsiloxane, diisostearyl malate, isododecane, diethylhexyl carbonate, dioctyl carbonate, lauroyl sarcosine isopropyl ester, isononyl isononanoate, hydrogenated polydecene, cetyl ethylhexanoate, bis-diethoxy diglycol cyclohexane 1, 4-dicarboxylate, caprylic/capric triglyceride, oleyl erucate, octyldodecanol myristate, capryloyl/capric triglyceride, and the like, Octyldodecanol, dimethicone, octylmethicone, cetyl dimethicone, cetostearyl ethyl hexanoate, avocado sterol, cyclopentadimethicone, cetyl alcohol, stearyl alcohol, cetostearyl alcohol, behenyl alcohol, batyl alcohol, lauric acid, shea butter, myristic acid, palmitic acid, stearic acid, beeswax, candelilla wax, carnauba wax, lanolin, ozokerite wax, jojoba seed wax, paraffin, petrolatum, microcrystalline wax, hydrogenated rice bran wax, hydrogenated coconut oil glyceride, myristyl myristate, bis-diglycerol polyacyladipate-2, shea butter, and mugwort palm seed fat.

In a preferred embodiment, for the type of fat, the invention is selected from one or more of hydrogenated polyisobutene, polydimethylsiloxane, beeswax, petrolatum, cetearyl alcohol, diethylhexyl carbonate, cyclopentadimethylsiloxane, isododecane and cetearyl ethyl hexanoate.

The amount of the oil or fat to be used is selected by those skilled in the art depending on the kind of the oil or fat, and for example, 0.5 to 5 parts by weight in the case of hydrogenated polyisobutene, 1 to 5 parts by weight in the case of polydimethylsiloxane, 0.1 to 2 parts by weight in the case of beeswax, 0.5 to 7 parts by weight in the case of petrolatum, and 0.5 to 1.5 parts by weight in the case of cetearyl alcohol, for example, may be used.

Perfume

In the present invention, the fragrance mainly serves to perfume the skin care product and has aesthetic value.

As perfume, natural or synthetic odoriferous substances or mixtures thereof may be used. Natural odoriferous substances include flowers (lily, lavender, rose, jasmine, orange blossom, ylang), stems and leaves (geranium, patchouli, orange leaf), fruits (fennel, coriander, caraway, juniper berry), husks (bergamot, lemon, citrus), roots (nutmeg, angelica, celery, cardamom, costus root, iris, thyme), needles and branches (spruce, fir, pine, mountain pine) and extracts of resins and balsams (boswellin, elemi, benzoin, myrrh, frankincense, red myrrh). Animal derived perfumes such as civet and castoreum may also be used. Typical synthetic fragrance compounds are ester, ether, aldehyde, ketone, alcohol and hydrocarbon type products. Ester-type fragrance compounds are, for example, benzyl acetate, phenoxyethyl isobutyrate, p-tert-butylcyclohexyl acetate, linalyl acetate, phenylethyl acetate, linalyl benzoate, benzyl formate, phenylglycine methyl ester, allylcyclohexyl propionate, styryl propionate and benzyl salicylate; ether-type fragrance compounds include, for example, benzyl ethyl ether; aldehyde-type fragrance compounds include, for example, linear alkanals having 8 to 18 carbon atoms, citral, citronellal, citronelloxy-acetaldehyde, cyclamen aldehyde, hydroxycitronellal, lilial and bourgeon aldehyde; ketone-type fragrance compounds include, for example, ionone, isomethylionone, and methyl cedryl ketone; alcohol-type perfume compounds include anisole, citronellol, eugenol, isoeugenol, geraniol, linalool, phenethyl alcohol and terpineol; and hydrocarbon-type perfume compounds mainly include terpenes and balsams. Mixtures of different scented substances may be used, which together produce an attractive scent. Volatile oils of low volatility are generally used as fragrance components and are also suitable as fragrances, for example sage oil, chamomile oil, clove oil, balm oil, peppermint oil, cinnamon leaf oil, basswood flower oil, juniper berry oil, vetiver oil, frankincense oil, Persian resin oil, labdanum oil and lavandin oil. Bergamot oil, dihydromyrcenol, lilial, lyral, citronellol, phenethyl alcohol, alpha-hexyl-cinnamaldehyde, geraniol, benzyl acetone, cyclamen, linalool, ambergris, indole, methyl dihydrojasmonate, lemon oil, mandarin oil, tangerine oil, allyl amyl glycolate, lavandin oil, sage oil, damascone, geranium oil, cyclohexyl salicylate, methyl cedryl ketone, phenylacetic acid, geranyl acetate, benzyl acetate, and rose oxide may be used alone or in mixtures.

In a preferred embodiment, the fragrance is selected from one or more of sage oil, chamomile oil, clove oil, balsam oil, peppermint oil, cinnamon leaf oil, basswood flower oil, juniper berry oil, vetiver oil, frankincense oil, Persian resin oil, labdanum oil and lavandin oil.

The amount of the perfume to be used can be selected by those skilled in the art according to the kind of the preservative.

Preservative

In the present invention, a preservative means a substance added to an agent composition for skin care for the purpose of inhibiting the growth and proliferation of microorganisms in the agent composition for skin care, to prolong the shelf life of the agent composition for skin care.

Preservatives in combinations of agents for skin care are classified in different ways. Common preservatives in combinations of agents for skin care are methylparaben, propylparaben, DMDM hydantoin, phenoxyethanol, glyceryl caprylate, ethylhexyl glycerin, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, caprylhydroxamic acid, and glyceryl caprylate.

The amount of the preservative to be used can be selected by those skilled in the art depending on the kind of the preservative, and for example, 0.1 to 2 parts by weight when phenoxyethanol is used and 0.1 to 1 part by weight when methylparaben is used can be used.

Skin care product

Unless specifically stated otherwise, the terms "first," "second," and "third" in the present invention are used merely to distinguish a plurality of similar elements, and are not intended to indicate any difference in importance or order between the elements.

In a preferred embodiment, the agent combination of the invention is comprised in a skin care product.

The skin care products of the present invention can include containers, instructions for use, use aids, fourth agents, fifth agents, and/or other structures and/or agents as desired for performing the performance test.

Other structures necessary for performing performance tests in the skin care products of the present invention include, but are not limited to, structures for sampling, structures for performing control, and/or structures for observing the progress or results of the assay.

The other agents required for performance testing in skin care products include, but are not limited to, culture media, detergents, developers and/or terminators, as well as thickeners, emulsifiers, surfactants, skin conditioners, oils, fragrances and preservatives other than those of the present invention.

Of course, it will be understood by those skilled in the art that the combination of agents of the present invention may contain, in addition to the components described above, other adjunct components such as free radical scavengers, chelating agents, antioxidants, film formers, stabilizers. These adjunct components are included in the first, second, and/or third reagents according to their solubility in the first, second, and third reagents.

The present invention will be described in more detail below with reference to examples, but the present invention is not limited to these examples.

Example 1

This example provides an agent combination for skin care comprising a first agent, a second agent, and a third agent, wherein, in parts by weight,

the first reagent comprises: 1 part of glycerol, 1 part of butanediol, 0.1 part of sodium hyaluronate and 80.8 parts of water;

the second reagent comprises: 1 part of tranexamic acid, 1 part of ascorbic acid, 0.1 part of dipotassium glycyrrhizinate and 14.9 parts of water;

the third reagent comprises: 0.1 part of umbilical cord mesenchymal stem cell exosome extract.

The preparation method of the agent combination for skin care of the embodiment is as follows:

step 1, adding 1 part of glycerol, 1 part of butanediol and 0.1 part of sodium hyaluronate into 80.8 parts of water, heating to 80 ℃, fully stirring and dissolving, and cooling to below 40 ℃ to obtain a first reagent;

step 2, adding 1 part of tranexamic acid, 1 part of ascorbic acid and 0.1 part of dipotassium glycyrrhizinate into 14.9 parts of water, and dissolving at the temperature of below 40 ℃ to obtain a second reagent;

and 3, fully stirring and uniformly mixing the first reagent and the second reagent, and adding 0.1 part of the umbilical cord mesenchymal stem cell exosome extract to obtain the skin-care reagent composition.

And 4, filtering the skin-care reagent combination with a 0.22-micrometer filter membrane under a hundred-grade laminar flow cover, and subpackaging into sterilized penicillin bottles.

Example 2

the first reagent comprises: 6 parts of glycerol, 6 parts of butanediol, 1.5 parts of sodium hyaluronate and 68.5 parts of water;

the second reagent comprises: 4 parts of tranexamic acid, 4 parts of ascorbic acid, 1 part of dipotassium glycyrrhizinate and 8 parts of water;

the third reagent comprises: 1 part of umbilical cord mesenchymal stem cell exosome extract.

step 1, adding 6 parts of glycerol, 6 parts of butanediol and 1.5 parts of sodium hyaluronate into 68.5 parts of water, heating to 80 ℃, fully stirring for dissolving, and cooling to below 40 ℃ to obtain a first reagent;

step 2, adding 4 parts of tranexamic acid, 4 parts of ascorbic acid and 1 part of dipotassium glycyrrhizinate into 8 parts of water, and dissolving at the temperature of below 40 ℃ to obtain a second reagent;

and 3, fully stirring and uniformly mixing the first reagent and the second reagent, and adding 1 part of the umbilical cord mesenchymal stem cell exosome extract to obtain the skin-care reagent composition.

Example 3

This example provides a lotion comprising a first agent, a second agent, and a third agent, wherein, in parts by weight,

The preparation method of the toning lotion comprises the following steps:

and 3, fully stirring and uniformly mixing the first reagent and the second reagent, and adding 0.1 part of the umbilical cord mesenchymal stem cell exosome extract to obtain the toning lotion.

And 4, filtering the lotion by using a 0.22-micron filter membrane under a hundred-grade laminar flow cover, and subpackaging into sterilized penicillin bottles.

Example 4

The preparation method of the toning lotion comprises the following steps:

and 3, fully stirring and uniformly mixing the first reagent and the second reagent, and adding 1 part of the umbilical cord mesenchymal stem cell exosome extract to obtain the toning lotion.

Example 5

The embodiment provides a facial mask, which comprises a first reagent, a second reagent and a third reagent, wherein according to parts by weight,

the first reagent comprises: 0.2 part of xanthan gum, 1 part of glycerol, 1 part of butanediol, 0.1 part of sodium hyaluronate, 0.1 part of methylparaben and 80.5 parts of water;

the second reagent comprises: 1 part of tranexamic acid, 1 part of ascorbic acid, 0.1 part of dipotassium glycyrrhizinate and 14.8 parts of water;

the third reagent comprises: 0.1 part of umbilical cord mesenchymal stem cell exosome extract and 0.2 part of phenoxyethanol.

The preparation method of the mask of the embodiment is as follows:

step 1, adding 0.2 part of xanthan gum, 1 part of glycerol, 1 part of butanediol, 0.1 part of sodium hyaluronate and 0.1 part of methylparaben into 80.5 parts of water, heating to 80 ℃, fully stirring and dissolving, and cooling to below 40 ℃ to obtain a first reagent;

step 2, adding 1 part of tranexamic acid, 1 part of ascorbic acid and 0.1 part of dipotassium glycyrrhizinate into 14.8 parts of water, and dissolving at the temperature of below 40 ℃ to obtain a second reagent;

step 3, fully stirring and uniformly mixing the first reagent and the second reagent, and adding 0.1 part of the umbilical cord mesenchymal stem cell exosome extract and 0.2 part of phenoxyethanol to obtain a mask liquid;

and 4, subpackaging the facial mask liquid into the membrane towel which is subjected to irradiation sterilization to obtain the facial mask.

Example 6

the first reagent comprises: 0.4 part of xanthan gum, 6 parts of glycerol, 6 parts of butanediol, 1.5 parts of sodium hyaluronate, 1 part of methylparaben and 66.5 parts of water;

the second reagent comprises: 4 parts of tranexamic acid, 4 parts of ascorbic acid, 1 part of dipotassium glycyrrhizinate and 7.6 parts of water;

the third reagent comprises: 1 part of umbilical cord mesenchymal stem cell exosome extract and 1 part of phenoxyethanol.

The preparation method of the mask of the embodiment is as follows:

step 1, adding 0.4 part of xanthan gum, 6 parts of glycerol, 6 parts of butanediol, 1.5 parts of sodium hyaluronate and 1 part of methylparaben into 66.5 parts of water, heating to 80 ℃, fully stirring and dissolving, and cooling to below 40 ℃ to obtain a first reagent;

step 2, adding 4 parts of tranexamic acid, 4 parts of ascorbic acid and 1 part of dipotassium glycyrrhizinate into 7.6 parts of water, and dissolving at the temperature of below 40 ℃ to obtain a second reagent;

step 3, fully stirring and uniformly mixing the first reagent and the second reagent, and adding 1 part of the umbilical cord mesenchymal stem cell exosome extract and 1 part of phenoxyethanol to obtain a mask liquid;

Example 7

This example provides an emulsion comprising a first reagent, a second reagent, a third reagent, and a fourth reagent, wherein, in parts by weight,

the first reagent comprises: 0.2 part of xanthan gum, 1 part of glycerol, 1 part of butanediol, 0.1 part of sodium hyaluronate, 0.1 part of methylparaben and 77.8 parts of water;

the second reagent comprises: 1 part of tranexamic acid, 1 part of ascorbic acid, 0.1 part of dipotassium glycyrrhizinate and 15.9 parts of water;

the third reagent comprises: 0.1 part of umbilical cord mesenchymal stem cell exosome extract and 0.1 part of phenoxyethanol;

the fourth reagent comprises: 0.1 part of polyglycerol-3 methyl glucose distearate, 0.5 part of hydrogenated polyisobutene, 1 part of polydimethylsiloxane.

The emulsion of this example was prepared as follows:

step 1, taking 0.1 part of polyglycerol-3 methyl glucose distearate, 0.5 part of hydrogenated polyisobutene and 1 part of polydimethylsiloxane, heating to 80 ℃, fully stirring and dissolving to obtain a fourth reagent, and preserving heat for later use;

step 2, adding 0.2 part of xanthan gum, 1 part of glycerol, 1 part of butanediol, 0.1 part of sodium hyaluronate and 0.1 part of methylparaben into 77.8 parts of water, heating to 80 ℃, fully stirring and dissolving, and cooling to below 40 ℃ to obtain a first reagent;

step 3, adding 1 part of tranexamic acid, 1 part of ascorbic acid and 0.1 part of dipotassium glycyrrhizinate into 15.9 parts of water, and dissolving at the temperature of below 40 ℃ to obtain a second reagent;

and 4, emulsifying the fourth reagent and the first reagent, cooling to below 40 ℃, adding the second reagent, 0.1 part of the umbilical cord mesenchymal stem cell exosome extract and 0.1 part of phenoxyethanol, and uniformly stirring to obtain the emulsion.

Example 8

This example provides an emulsion comprising a first agent, a second agent, a third agent, and a fourth agent, wherein, in parts by weight,

the first reagent comprises: 0.4 part of xanthan gum, 6 parts of glycerol, 6 parts of butanediol, 1.5 parts of sodium hyaluronate, 1 part of methylparaben and 54.1 parts of water;

the third reagent comprises: 1 part of umbilical cord mesenchymal stem cell exosome extract and 2 parts of phenoxyethanol;

the fourth reagent comprises: 1 part of polyglycerol-3 methyl glucose distearate, 5 parts of hydrogenated polyisobutene and 5 parts of polydimethylsiloxane.

The emulsion of this example was prepared as follows:

step 1, heating 1 part of polyglycerol-3 methyl glucose distearate, 5 parts of hydrogenated polyisobutene and 5 parts of polydimethylsiloxane to 80 ℃, fully stirring and dissolving to obtain a fourth reagent, and preserving heat for later use;

step 2, adding 0.4 part of xanthan gum, 6 parts of glycerol, 6 parts of butanediol, 1.5 parts of sodium hyaluronate and 1 part of methylparaben into 54.1 parts of water, heating to 80 ℃, fully stirring and dissolving, and cooling to below 40 ℃ to obtain a first reagent;

step 3, adding 4 parts of tranexamic acid, 4 parts of ascorbic acid and 1 part of dipotassium glycyrrhizinate into 8 parts of water, and dissolving at the temperature of below 40 ℃ to obtain a second reagent;

and 4, emulsifying the fourth reagent and the first reagent, cooling to below 40 ℃, adding the second reagent, 1 part of the umbilical cord mesenchymal stem cell exosome extract and 2 parts of phenoxyethanol, and uniformly stirring to obtain the emulsion.

Example 9

This example provides a cream comprising a first agent, a second agent, a third agent, and a fourth agent, wherein, in parts by weight,

the first reagent comprises: 0.2 part of xanthan gum, 1 part of glycerol, 1 part of butanediol, 0.1 part of sodium hyaluronate, 0.1 part of methylparaben and 75.8 parts of water;

the fourth reagent comprises: 1 part of cetearyl alcohol and cetearyl glucoside, 0.5 part of hydrogenated polyisobutene, 1 part of polydimethylsiloxane, 0.1 part of beeswax, 0.5 part of petrolatum, 0.5 part of cetearyl alcohol.

The cream of this example was prepared as follows:

step 1, taking 1 part of cetearyl alcohol and cetearyl glucoside, 0.5 part of hydrogenated polyisobutene, 1 part of polydimethylsiloxane, 0.1 part of beeswax, 0.5 part of petrolatum and 0.5 part of cetearyl alcohol, heating to 80 ℃, fully stirring and dissolving to obtain a fourth reagent, and preserving heat for later use;

step 2, adding 0.2 part of xanthan gum, 1 part of glycerol, 1 part of butanediol, 0.1 part of sodium hyaluronate and 0.1 part of methylparaben into 75.8 parts of water, heating to 80 ℃, fully stirring and dissolving, and cooling to below 40 ℃ to obtain a first reagent;

and 4, emulsifying the fourth reagent and the first reagent, cooling to below 40 ℃, adding the second reagent, 0.1 part of the umbilical cord mesenchymal stem cell exosome extract and 0.1 part of phenoxyethanol, and uniformly stirring to obtain the cream.

Example 10

the first reagent comprises: 0.4 part of xanthan gum, 6 parts of glycerol, 6 parts of butanediol, 1.5 parts of sodium hyaluronate, 1 part of methylparaben and 43.6 parts of water;

the second reagent comprises: 4 parts of tranexamic acid, 4 parts of ascorbic acid, 1 part of dipotassium glycyrrhizinate and 9 parts of water;

the fourth reagent comprises: 3 parts of cetearyl alcohol and cetearyl glucoside, 5 parts of hydrogenated polyisobutene, 5 parts of polydimethylsiloxane, 2 parts of beeswax, 7 parts of petrolatum, 1.5 parts of cetearyl alcohol.

The cream of this example was prepared as follows:

step 1, taking 3 parts of cetearyl alcohol and cetearyl glucoside, 5 parts of hydrogenated polyisobutene, 5 parts of polydimethylsiloxane, 2 parts of beeswax, 7 parts of petrolatum and 1.5 parts of cetearyl alcohol, heating to 80 ℃, fully stirring and dissolving to obtain a fourth reagent, and preserving heat for later use;

step 2, adding 0.4 part of xanthan gum, 6 parts of glycerol, 6 parts of butanediol, 1.5 parts of sodium hyaluronate and 1 part of methylparaben into 43.6 parts of water, heating to 80 ℃, fully stirring and dissolving, and cooling to below 40 ℃ to obtain a first reagent;

step 3, adding 4 parts of tranexamic acid, 4 parts of ascorbic acid and 1 part of dipotassium glycyrrhizinate into 9 parts of water, and dissolving at the temperature of below 40 ℃ to obtain a second reagent;

and 4, emulsifying the fourth reagent and the first reagent, cooling to below 40 ℃, adding the second reagent, 1 part of the umbilical cord mesenchymal stem cell exosome extract and 2 parts of phenoxyethanol, and uniformly stirring to obtain the cream.

Comparative example 1

To further illustrate the beneficial effects of the present invention, a moisturizing, wrinkle-removing and antiallergic composition is provided, which is prepared by the method in patent CN 108354865A.

Comparative example 2

To further illustrate the beneficial effects of the present invention, comparative example 2 is provided, which comparative example 2 differs from example 3 in that: the butanediol of the present invention is replaced with xylitol.

Comparative example 2 provides a lotion comprising a first agent, a second agent, and a third agent, wherein, in parts by weight,

the first reagent comprises: 1 part of glycerol, 1 part of xylitol, 0.1 part of sodium hyaluronate and 80.8 parts of water;

Comparative example 2 a toner was prepared as follows:

step 1, adding 1 part of glycerol, 1 part of xylitol and 0.1 part of sodium hyaluronate into 80.8 parts of water, heating to 80 ℃, fully stirring and dissolving, and cooling to below 40 ℃ to obtain a first reagent;

Comparative example 3

To further illustrate the beneficial effects of the present invention, comparative example 3 is provided, which comparative example 3 differs from example 3 in that: the dipotassium glycyrrhizinate of the invention is replaced by ubiquinone.

Comparative example 3 provides a lotion comprising a first agent, a second agent, and a third agent, wherein, in parts by weight,

the second reagent comprises: 1 part tranexamic acid, 1 part ascorbic acid, 0.1 part ubiquinone and 14.9 parts water;

Comparative example 3a toner was prepared as follows:

step 2, adding 1 part of tranexamic acid, 1 part of ascorbic acid and 0.1 part of ubiquinone into 14.9 parts of water, and dissolving at the temperature of below 40 ℃ to obtain a second reagent;

Experiment 1: wrinkle removal test

A subject: 130 volunteers with 20-60 years old and severe raised lines and canthus lines are divided into 13 groups, and each group contains 10 persons;

the test method comprises the following steps: samples of examples 1-10 and comparative examples 1-3 are respectively taken and smeared on the face, the samples are respectively used once in the morning and at the evening for 60 days continuously, and a skin diagnostician-Visia is used for wrinkle detection; the results are shown in Table 1.

Experiment 2: antiallergic test

A subject: 130 volunteers with skin of 20-60 years old in allergic phase, divided into 13 groups of 10 persons each;

the test method comprises the following steps: samples of examples 1-10 and comparative examples 1-3 are respectively taken and smeared on the face, the samples are respectively used once in the morning and at the evening for 60 days continuously, and a skin diagnostician-Visia is used for detecting allergic regions; the results are shown in Table 1.

TABLE 1

Remarking: the number of people with reduced wrinkles is 50% or more;

the allergy-reducing population is a population having an allergy reduction rate of 50 or more.

From the above results, it can be seen that:

the umbilical cord mesenchymal stem cell exosome used in the reagent combination can simulate partial functions of umbilical cord mesenchymal stem cells to play roles of promoting tissue repair and immunoregulation, has small molecular weight, and enables the skin to have better absorbability, and the specific tranexamic acid, ascorbic acid and dipotassium glycyrrhizinate matched with the reagent combination can better improve dry or damaged skin, and can better resist allergy, diminish inflammation and relieve the skin; specifically, by using the skin care reagent combination provided by the invention, the wrinkle reduction ratio can reach more than 70%, and the allergy reduction ratio can reach as high as 80%, which indicates that the skin care reagent combination has the technical effects of better wrinkle removal, better anti-allergy property and better absorbability by screening the types of the components in the skin care reagent combination or adjusting the weight parts of the components in the skin care reagent combination. The existing skin care products have certain wrinkle removing effect, but the anti-allergic effect is poor, and the skin condition of people with skin allergy cannot be well improved.

Similarly, the umbilical cord mesenchymal stem cell exosome used in the toning lotion can simulate partial functions of umbilical cord mesenchymal stem cells to play roles of promoting tissue repair and immunoregulation, and has small molecular weight, so that the skin absorbability is better, and the dried or damaged skin can be better improved by matching the specific tranexamic acid, the ascorbic acid and the dipotassium glycyrrhizinate, so that the skin can be better antiallergic, anti-inflammatory and soothing; specifically, by using the toning lotion provided by the invention, the wrinkle reduction ratio can reach more than 80%, and the allergy reduction ratio can reach 80%, which shows that the toning lotion has the technical effects of better wrinkle removal, better antianaphylaxis and better absorbability by screening the types of the components in the toning lotion or adjusting the weight parts of the components in the toning lotion.

Similarly, the umbilical cord mesenchymal stem cell exosome used in the facial mask can simulate partial functions of umbilical cord mesenchymal stem cells to play roles of promoting tissue repair and immunoregulation, and has small molecular weight, so that the skin absorbability is better, and the dried or damaged skin can be better improved by matching the specific tranexamic acid, the ascorbic acid and the dipotassium glycyrrhizinate, so that the skin can be better antiallergic, anti-inflammatory and soothing; specifically, by using the mask provided by the invention, the wrinkle reduction ratio can be up to more than 80%, and the allergy reduction ratio can be up to 80%, which shows that the mask has the technical effects of better wrinkle removal, better antianaphylaxis and better absorbability by screening the types of the components in the mask or adjusting the weight parts of the components in the mask.

Similarly, the umbilical cord mesenchymal stem cell exosome used in the emulsion can simulate partial functions of umbilical cord mesenchymal stem cells to play roles of promoting tissue repair and immunoregulation, and has small molecular weight, so that the skin absorbability is better, and the dried or damaged skin can be better improved by matching the specific tranexamic acid, the ascorbic acid and the dipotassium glycyrrhizinate, so that the skin can be better antiallergic, anti-inflammatory and soothing; specifically, by using the emulsion provided by the invention, the wrinkle reduction ratio can reach more than 90%, and the allergy reduction ratio can reach 90%, which shows that the emulsion has the technical effects of better wrinkle removal, better antianaphylaxis and better absorbability by screening the types of the components in the emulsion or adjusting the weight parts of the components in the emulsion.

Similarly, the umbilical cord mesenchymal stem cell exosome used in the cream can simulate partial functions of umbilical cord mesenchymal stem cells to play roles of promoting tissue repair and immunoregulation, and has small molecular weight, so that the skin absorbability is better, and the dried or damaged skin can be better improved by matching the specific tranexamic acid, the ascorbic acid and the dipotassium glycyrrhizinate, so that the skin can be better antiallergic, anti-inflammatory and soothing; specifically, by using the cream provided by the invention, the wrinkle reduction ratio can reach more than 80%, and the allergy reduction ratio can reach 90%, which shows that the cream has the technical effects of better wrinkle removal, better antianaphylaxis and better absorbability by screening the types of the components in the cream or adjusting the weight parts of the components in the cream.

It is to be understood that the invention disclosed is not limited to the particular methodology, protocols, and materials described, as these may vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments only, and is not intended to limit the scope of the present invention which will be limited only by the appended claims.

Those skilled in the art will also recognize, or be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Claims

1. An agent combination for skin care comprising a first agent, a second agent and a third agent, wherein,

the first agent comprises glycerol, butylene glycol, and sodium hyaluronate;

2. The reagent combination according to claim 1, wherein the reagent combination comprises, in parts by weight,

the first agent comprises 1-6 parts of glycerol, 1-6 parts of butanediol and 0.1-1.5 parts of sodium hyaluronate.

3. The reagent combination according to claim 1, wherein the reagent combination comprises, in parts by weight,

the second reagent comprises 1-4 parts of tranexamic acid, 1-4 parts of ascorbic acid and 0.1-1 part of dipotassium glycyrrhizinate.

4. The reagent combination according to claim 1, wherein the reagent combination comprises, in parts by weight,

5. An agent combination for skin care comprising a first agent, a second agent and a third agent, wherein the first agent, the second agent and the third agent are, in parts by weight,

6. The combination of agents of claim 1, the first agent further comprising one or more of a thickening agent, an emulsifier, a surfactant, a skin conditioner, a grease, a fragrance, and a preservative, wherein,

the thickening agent is one or more selected from carbomer, xanthan gum, sodium carrageenan, Arabic gum, polyethylene glycol-14M, polyethylene glycol-90M, succinoglycan, SEPIPLUS 265, hydroxyethyl cellulose, hydroxypropyl cellulose and hydroxypropyl methyl cellulose;

the emulsifier is selected from the group consisting of cetearyl olivate, sorbitan olivate, polysorbate-60, polysorbate-80, polysorbate-85, methylgluco-sesquistearate, PEG-20 methylgluco-sesquistearate, PEG-40 hydrogenated castor oil, PPG-26-buteth-26, PEG-4 polyglyceryl-2 stearate, PEG-60 hydrogenated castor oil, steareth-2, steareth-21, PPG-13-decyltetraeth-24, cetearyl glucoside, PEG-100 stearate, ceteth-20, glyceryl stearate SE, coco glucoside, ceteth-25, PEG-40 stearate, polyglyceryl-3-methylgluco-distearate, PEG-4 polyglyceryl-2 stearate, PEG-60 hydrogenated castor oil, ceteth-2, ceteth-21, PPG-13-decylteth-24, ceteth-100 stearate, ceteth-20, glyceryl stearate SE, coco, One or more of glyceryl stearate citrate, polyglyceryl-10 stearate, polyglyceryl-10 myristate, polyglyceryl-10 dioleate, polyglyceryl-10 laurate, polyglyceryl-10 isostearate, polyglyceryl-10 oleate, polyglyceryl-10 diisostearate, polyglyceryl-6 laurate, polyglyceryl-6 myristate, sucrose distearate and sucrose polystearate;

the surfactant is selected from one or more of Tween, SPAN, Triton, EMULGEN series surfactant, laurin polyethylene glycol glyceride, anionic alkyl polyglycoside, polyglycerol-3 methyl glucose distearate, cetearyl alcohol and cetearyl glucoside, dodecyl dimethyl betaine, 3ARAMT1, SHO62C and AM PHITOL;

the skin conditioning agent is selected from kojic acid, ascorbic acid, ascorbyl glucoside, sodium hyaluronate, arbutin, tranexamic acid, niacinamide, phytosterol, phenethyl resorcinol, turmeric root extract, birch bark extract, ceramide 2, ceramide 3, panthenol, acetyl phytosphingosine, dipotassium glycyrrhizinate, carboxymethyl chitosan, trehalose, glycerin, hydroxyethyl urea, propylene glycol, beta-glucan, glyceryl polyether-26, allantoin, ethanol, resveratrol, Pterocarpus marsupium bark extract, Coleus forskohlii root extract, pepper seed extract, glyceryl polyacrylate, warm water, Tremella entity extract, ubiquinone, cholesterol stearate, ascorbyl dipalmitate, tocopherol, tocopheryl acetate, bisabolol, ascorbyl tetraisopalmitate, pyridoxine dioctanoate, One or more of pyridoxine dipalmitate, retinol palmitate and the phytosterol macadamia oleate;

the oil and fat is selected from olive oil, macadamia nut oil, sweet almond oil, mineral oil, grape seed oil, avocado oil, corn oil, sesame oil, soybean oil, peanut oil, meadowfoam seed oil, safflower seed oil, rosa canina oil, anise oil, argan oil, jojoba oil, sunflower seed oil, oil of ruined palm fruit, squalane, ethylhexyl palmitate, isopropyl myristate, hydrogenated polyisobutene, isohexadecane, jojoba esters, polydimethylsiloxane, diisostearyl malate, isododecane, diethylhexyl carbonate, dioctyl carbonate, isopropyl lauroyl sarcosinate, isononyl isononanoate, hydrogenated polydecene, cetyl ethylhexanoate, bis-diethoxy diglycol cyclohexane 1, 4-dicarboxylate, oleyl erucate, octyldodecanol myristate, octyldodecanol, polydimethylsiloxane, erucic acid ester, Octyl methicone, cetyl dimethicone, cetostearyl ethyl hexanoate, avocado sterol, cyclopentadimethicone, cetyl alcohol, stearyl alcohol, cetostearyl alcohol, behenyl alcohol, batyl alcohol, lauric acid, shea butter, myristic acid, palmitic acid, stearic acid, beeswax, candelilla wax, carnauba wax, lanolin, ozokerite wax, jojoba seed wax, paraffin, petrolatum, microcrystalline wax, hydrogenated rice bran wax, hydrogenated coconut oil glycerides, myristyl myristate, bis-diglycerol polyacyladipate-2, shea butter, and pluronic palm seed fat;

the perfume is selected from one or more of sage oil, chamomile oil, clove oil, balsam oil, peppermint oil, cinnamon leaf oil, linden flower oil, juniper berry oil, vetiver oil, frankincense oil, Persian resin oil, labdanum oil and lavandin oil;

the preservative is selected from one or more of methyl hydroxybenzoate, propyl hydroxybenzoate, DMDM hydantoin, phenoxyethanol, glyceryl caprylate, ethylhexyl glycerol, benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, caprylyl hydroxamic acid and glyceryl caprylate.

7. The combination of agents of claim 1, the second agent further comprising one or more of a thickening agent, an emulsifier, a surfactant, a skin conditioner, a grease, a fragrance, and a preservative, wherein,

8. The combination of agents of claim 1, the third agent further comprising one or more of a thickening agent, an emulsifier, a surfactant, a skin conditioner, a grease, a fragrance, and a preservative, wherein,

9. A skin care product comprising the combination of agents of any one of claims 1-8, wherein the skin care product is a lotion, an emulsion, a cream, or a mask.

10. A pharmaceutical composition comprising a safe and effective amount of a combination of agents as claimed in any one of claims 1 to 8.

11. Use of the combination of agents according to any one of claims 1 to 8 for the preparation of a skin care product.

12. Use of a combination of agents according to any one of claims 1 to 8 for the preparation of a pharmaceutical composition.