CN1119972C - Coaxle low-light fluorescent diagnosing and positioning instrument for cancer of lung - Google Patents
Coaxle low-light fluorescent diagnosing and positioning instrument for cancer of lung Download PDFInfo
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- CN1119972C CN1119972C CN 99121052 CN99121052A CN1119972C CN 1119972 C CN1119972 C CN 1119972C CN 99121052 CN99121052 CN 99121052 CN 99121052 A CN99121052 A CN 99121052A CN 1119972 C CN1119972 C CN 1119972C
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Abstract
The present invention relates to lung cancer, particularly to a diagnostic positioning technique for earlier lung cancer, which belongs to the class of medical appliances. The present invention transfers laser light used for excitation to a target site to be examined and simultaneously makes white light used for illumination to the target site after the white light is decayed to the magnitude of weak light. Backward diffuse reflection light formed from induced medicine fluorescence after laser radiation and weak illumination light reflected from an examined target tissue forms fluorescence images and weak light images with amplified brightness by an image enhancing device through a coaxial light path to respectively realize real-time diagnoses and positioning. Because of the adoption of weak light illumination, the present invention has the advantages of greatly decelerating the photobleaching rate of photosensitizers in the target tissue, increasing the detectable time of focus and improving the sensitivity of system detection.
Description
The present invention relates to pulmonary cancer diagnosis and location technology, belong to the Medical Instruments class.
In clinical diagnosis medical science, white light bronchoscopy art is a kind of effective means of diagnosis and location lung tumors.During clinical diagnosis, operator utilize workina ocular,, pass the image color that resembles bundle observation lung tissue by optical fibers and change as illumination light with white light, according to microscopy doctor's working experience, seek pathological tissues, diagnosis and location lung tumors.But white light bronchoscope diagnostic procedure is subjected to doctor and patient individual difference's influence, and it is very accurate to be difficult to accomplish, and can not observe early stage pulmonary cancer in situ.
Fiber bronchoscope fluorescence imaging system by people such as U.S. Profio exploitation can diagnose the early stage of lung cancer.This system utilizes photosensitive drug hematoporphyrin derivative HPD and malignant tumor to have special affinity, and in a period of time of giving after patient injects HPD in advance, the HPD concentration of institute's retention forms significant difference in tumor tissues and the normal structure.Under purple laser irradiation, tumor locus is salmon pink fluorescence, therefore this phenomenon also can be confirmed by the fluorescence spectrum characteristic of tumor tissues and normal structure, and the brightness of observing red fluorescence by endoscope's fluorescence imaging device can realize diagnosis and the location to lung tumors.The doctor is doing to patient in the fluorescence bronchoscope checking process, at first will guide the point of endoscope to make it to enter into each different parts of patient pulmonary with white light, and the reuse laser beam excites lung tissue to carry out fluoroscopy.Utilize this technology can detect diameter less than 2.0mm, thickness early stage cancer in situ for 0.1mm.
Calculate and experimental result shows that above-mentioned diagnostic system fluorescence signal intensity during from the white light signal intensity of focus and fluoroscopy when carrying out the conventional white light inspection differs 4～5 more than the magnitude, fluorescence signal is very faint, visual luminance level well below the normal eye, need carry out processing and amplifying with casting image intensifier, and white light signal can not directly put on casting image intensifier too by force.In order to address this problem, the laser that the present invention will be used to excite is sent to tested target position, and the white light that will be used to throw light on arrives this target position after decaying to low light level magnitude simultaneously.Medicine fluorescence and the back of tested target tissue reflection weak illumination light formation inductive after the laser irradiation form fluorescent image and the low-light image that brightness is exaggerated to diffusing through coaxial optical routing casting image intensifier, realize real-time diagnosis and location respectively.
The present invention is achieved through the following technical solutions.The white light that white light source sends (after 0～7V) decay, is 10 through attenuation quotient through regulating driving voltage again
-2～10
-3The attenuator decay of magnitude makes tested lung tissue target position surface obtain the dimmed level illumination.By endoscope lens gather the target position tissue simultaneously back to diffusing and medicine fluorescence, pass via endoscope simultaneously and resemble bundle, coaxial low-light-fluorescence light path system imaging on the time of casting image intensifier pole-face.Red arrowband grating filter is housed between convertible lens and the endoscope's eyepiece in coaxial low-light-fluorescence light path system.This optical filter can be the fluorescence of 690nm by wavelength to greatest extent, forms the low-light of starlight level after two magnitudes that the low light level can be decayed again simultaneously.Low-light and fluorescence amplify about 10 through casting image intensifier
4Magnitude by real-time switching white light on and off switch, can be known low-light image or the fluorescent image of seeing on the casting image intensifier fluorescent screen in eyepiece or shooting print record, to reach above-mentioned tested lung tissue is implemented diagnosis and localized purpose.
Concrete structure of the present invention is provided by following embodiment and accompanying drawing thereof.
Fig. 1 is concrete structure figure of the present invention.
Fig. 2 is the coaxial low-light of the present invention-fluorescence light path system and white light source is controlled and transmission system.
Coaxial low-light-fluorescence pulmonary cancer diagnosis and location instrument carry out effectively fixing and adjusting by static organ (9) among Fig. 1.The white light source of controlled light intensity (6) becomes the low light level by the voltage regulator (20) among Fig. 2 (regulating driving voltage between 0～7V), white light condenser lens (22) with optical attenuation sheet (23), and the point that enters endoscope (8) by white light optical fibers (24) makes it to be radiated at each different parts of patient pulmonary.The focal length of white light condenser lens (22) is 12mm, and the attenuation quotient of optical attenuation sheet (23) is 10
-2～10
-3The purple laser that krypton ion laser among Fig. 1 (2) sends in real time behind purple optical filter (3) and coupled lens (4), is transferred to exciting light on the tested target position tissue (7) by silicon fiber (5).What endoscope lens among Fig. 2 (14) was gathered target position tissue (7) simultaneously is back to diffusing and medicine fluorescence, simultaneously via optical fibers pass resemble bundle (15) enter into coaxial low-light-fluorescence light path system (10) also imaging on the time of casting image intensifier (19) pole-face.Between endoscope's eyepiece (16) and the convertible lens (18) red arrowband grating filter (17) is housed in coaxial low-light-fluorescence light path system (10).This optical filter (17) can be the fluorescence of 690nm by wavelength to greatest extent, simultaneously the back is formed the low-light of starlight level after two magnitudes of decay that diffuse, thereby the cathode plane that has guaranteed casting image intensifier (19) can receive fluorescent image and low-light image simultaneously.Black and white fluorescent image on casting image intensifier (19) cathode plane or low-light image are converted into video signal by photographic head (11) at last and enter computer image treatment and show print system (1), wherein fluorescent image is handled and is adopted converter technique pseudo-colours image treatment technology, and the pseudo-colours image of demonstration can print as required in real time.
Before clinical diagnosis, the patient is by the medicinal photosensitizer (HPD) of intravenous injection doses, diagnoses with this instrument after (common 8 to 72 hours) at interval and locatees through a period of time.In the course of the work, location anatomical position and fluorescence diagnosis hocket.Krypton ion laser (2) is in normally open, helps laser instrument and runs well.Open the switch (21) of white light source (6), regulate driving voltage actuator (20), driving voltage is adjusted to 5V, white light beam is through condenser lens (22) and optical attenuation sheet (23), make magnitude of white light light intensity attenuation form the low light level, be transferred on the tested lung tissue body target position (7) by white light optical fibers (24), realize low light level illumination.Compare with traditional white light direct irradiation, slowed down the photosensitizer photobleaching speed that detects in the tissue widely.Tested lung tissue body target position (7) is as the low light level and exciting light working flare, through bronchus endoscope (8), and red arrowband grating filter (17), convertible lens (18) imaging is on the time of ITT night vision casting image intensifier (19) pole-face.Red arrowband grating filter (17) is to two magnitudes of low light level light intensity attenuation, and obtain starlight level illumination this moment on casting image intensifier (19) the time pole-face, see the low-light image that brightness is amplified on fluorescent screen.In view of the above, can accurately locate the anatomical position of branchofiberoscope visual field lung tissue of living in.Afterwards, close white light source switch (20), purple laser beam by Kr laser (2) output, behind purple optical filter (3) and coupled lens (4), entering silicon fiber (5) transmits, light beam (spot size is consistent with bronchoscope objective line visual field) by the terminal self-focusing microlens output of silicon fiber (5) is radiated on the tested lung tissue target position (7) derivative medicine fluorescence imaging light path and the coaxial same light path of above-mentioned low-light imaging light path.Can see fluorescent image clearly this moment on the cathode plane of casting image intensifier, reach the purpose of the early stage cancer in situ of diagnosis.Search in the process of tumor tissues in fluoroscopy, when finding tumor tissues fluorescence,, also need open the switch (21) of white light source (6), introduce the low light level in real time and observe the anatomical position of low-light image with the location focus in order to determine the anatomical position of focus.As mentioned above, utilize this device, can directly realize the Real Time Observation of low-light image or fluorescent image by control white light source switch (21).In diagnostic procedure, the camera head of the also available band pick-up lens of doctor, the direct observation low-light resembles or fluorescent image on JVC monitor 12, and system's operation is very easy.
The present invention adopts low light level illumination, compares with traditional white light direct irradiation, has slowed down the photobleaching speed of photosensitizer in the target tissue widely, has improved the sensitivity that system detects.Simultaneously, back two magnitudes that after diffusing, decay on the target tissue through the red arrowband grating filter in coaxial low-light one fluorescent apparatus, form the low-light of starlight level, thereby the cathode plane that guarantees casting image intensifier can receive fluorescent image and low-light image simultaneously, realizes the real-time diagnosis and the location of early stage pulmonary cancer in situ respectively.The black and white fluorescent image is converted into color to the black-and-white image of reflection fluorescence intensity with the pseudo-colours image that fluorescence intensity changes through after the Computer Processing, has improved the distinguishing ability of clinician to image greatly.Compare with existing pulmonary cancer diagnosis means, this instrument is not only highly sensitive, and image quality is good, and diagnosis rapidly, and the result is accurate.
Claims (4)
1. a pulmonary cancer diagnosis and location instrument of forming by LASER Light Source and transmission system, white light source and transmission system and endoscope's casting image intensifier imaging system, it is characterized in that this instrument by contain driving voltage between 0～7V and the white light source that can regulate continuously and transmission system and coaxial low-light-fluorescence light path system that red arrowband grating filter is housed form.
2. instrument according to claim 1, the focal length that it is characterized in that white light condenser lens (22) in white light source and the transmission system is 12mm, the attenuation quotient of optical attenuation sheet (23) is 10
-2～10
-3
3. instrument according to claim 1, it is characterized in that being to be equipped with on coaxial low-light-fluorescence light path system (10) the red arrowband grating filter (17) between endoscope's eyepiece (16) and convertible lens (18), this optical filter can be the fluorescence of 690nm by wavelength to greatest extent, again with forming the low-light of starlight level after two magnitudes of low light level decay, make the cathode plane of casting image intensifier (19) can receive fluorescent image and low-light image simultaneously simultaneously.
4. instrument according to claim 3 is characterized in that casting image intensifier (19) is in normally open, only need by the control white light source open the light (21) realize alternately conversion in real time between low-light image and the fluorescent image.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99121052 CN1119972C (en) | 1999-11-13 | 1999-11-13 | Coaxle low-light fluorescent diagnosing and positioning instrument for cancer of lung |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99121052 CN1119972C (en) | 1999-11-13 | 1999-11-13 | Coaxle low-light fluorescent diagnosing and positioning instrument for cancer of lung |
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| Publication Number | Publication Date |
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| CN1251290A CN1251290A (en) | 2000-04-26 |
| CN1119972C true CN1119972C (en) | 2003-09-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 99121052 Expired - Fee Related CN1119972C (en) | 1999-11-13 | 1999-11-13 | Coaxle low-light fluorescent diagnosing and positioning instrument for cancer of lung |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6821289B2 (en) * | 2003-03-25 | 2004-11-23 | Ceramoptec Industries, Inc. | Efficacy and safety of photodynamic therapy by multiple application protocols with photosensitizers that show extended tumor retention |
| CN101518674B (en) * | 2009-03-13 | 2012-07-04 | 深圳先进技术研究院 | Visualization photic stimulation system and visualization photic stimulation method |
| CN103385693A (en) * | 2012-05-09 | 2013-11-13 | 朱瑞龙 | Tumor imaging device |
| CN103315699A (en) * | 2013-06-13 | 2013-09-25 | 福建师范大学 | Endoscopic system applicable to positioning and recognition of Barrett's esophagus |
| CN103932673B (en) * | 2014-04-24 | 2016-05-04 | 福建师范大学 | A kind of arthroscope system based on multimode spectral technique |
| CN110115557B (en) * | 2019-05-17 | 2021-12-14 | 中国科学院大学 | Hyperspectral endoscopic imaging device and imaging method |
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1999
- 1999-11-13 CN CN 99121052 patent/CN1119972C/en not_active Expired - Fee Related
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