CN111977903A - Biological treatment method for blue algae - Google Patents
Biological treatment method for blue algae Download PDFInfo
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- CN111977903A CN111977903A CN202010840720.4A CN202010840720A CN111977903A CN 111977903 A CN111977903 A CN 111977903A CN 202010840720 A CN202010840720 A CN 202010840720A CN 111977903 A CN111977903 A CN 111977903A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/302—Nitrification and denitrification treatment
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/30—Aerobic and anaerobic processes
- C02F3/308—Biological phosphorus removal
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/001—Processes for the treatment of water whereby the filtration technique is of importance
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- C02F2001/007—Processes including a sedimentation step
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
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Abstract
The invention belongs to the technical field of water body environment treatment, and relates to a blue algae biological treatment method, which sequentially comprises the following steps: step one, adopting microorganism control; step two: adopting microorganism inhibition; step three: degrading algal toxins generated after blue algae die by using microorganisms; step four: precipitating and filtering the residual impurities of the blue algae in the water body.
Description
Technical Field
The invention belongs to the technical field of water body environment treatment, and relates to a blue algae biological treatment method.
Background
Blue algae outbreaks are a worldwide environmental problem. In eutrophic water, when the conditions favorable for the growth of blue algae, such as proper water temperature, nitrogen-phosphorus ratio and the like, occur, the blue algae can propagate in large quantity, and the characteristics of annual diffusion of outbreak area and annual prolongation of duration are presented. The blue algae outbreak destroys the ecological balance of the water body, deteriorates the ecological landscape of the water body and reduces the available resources of the available fresh water. Moreover, the blue algae can continuously secrete toxic metabolites to the water body in the propagation process, so that population succession and the propagation period of plankton are influenced, and a large amount of floating animals can be killed; the death and decomposition of blue algae consumes a large amount of dissolved oxygen, and releases a large amount of toxic substances such as hydroxylamine, sulfide and the like, so that a large amount of fishes and shrimps are difficult to live and die.
Part of the specific areas in the blue algae are filled with algal toxins, which can be divided into hepatotoxins and neurotoxins through the harm mode, and the toxins which attack the liver and nerves, and the other toxins have stimulation to the skin. When the cyanobacteria cells are broken or dead, the toxins can be released into the water, and if the cyanobacteria cells are drunk for a long time, the water polluted by the algae can have adverse effects on the growth period or the chronic period of the human body even if the content is low. Therefore, the treatment and control of blue algae is a serious problem.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides a method for biologically treating blue algae, which solves the problem that the water quality is polluted because a large amount of toxins are released in water after the blue algae in the water body is excessively propagated and dies.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a blue algae biological treatment method is characterized in that: comprises the following steps in sequence
Step one, adopting microorganism control;
step two: adopting microorganism inhibition;
step three: degrading algal toxin generated after blue algae death by adopting a microbial strain;
step four: precipitating and filtering the residual impurities of the blue algae in the water body.
Further, biological nitrogen removal and biological phosphorus removal are sequentially adopted for biological control.
Further, the biological nitrogen removal comprises ammoniation-nitrification-denitrification, wherein ammoniation is to perform oxidative deamination by using ammoniation enzyme under an aerobic condition, nitrification is to oxidize NH3-N into NxO-N by using nitrite bacteria and nitrate bacteria, denitrification is to reduce nitrate into nitrogen so as to reduce the content of nitrogen nutrition in a water body and eliminate the toxic action on organisms due to nitric acid accumulation, and the biological phosphorus removal is performed under an aerobic or anoxic state.
Further, a high-activity catalyst for promoting ammoniation is added in the ammoniation process, and the adding amount of the high-activity catalyst is 0.85g.L-1-1.25g.L-1。
Further, the biological phosphorus removal is implemented by adopting phosphorus-accumulating bacteria which are specifically cultured and enhanced by genes, wherein a trace element additive is added into the phosphorus-accumulating bacteria, and the ratio of the phosphorus-accumulating bacteria to the trace element additive is 1: 1-5.
Furthermore, the microorganism inhibition adopts algicidal bacteria, and the algicidal bacteria inhibit the blue algae.
Further, the algicidal bacteria directly act or indirectly act or synergistically act on the blue algae.
Further, the microorganism strains comprise one or more of sphingomonas, methylobacterium, lactobacillus strains, dirofibacterium lactis strains and lactobacillus plantarum strains.
Furthermore, quartz sand is adopted for filtering the sediment of the blue algae residual impurities in the water body.
Furthermore, fiber filtration is adopted for precipitating and filtering the residual impurities of the blue algae in the water body.
As can be seen from the above description of the present invention, compared with the prior art, the method for treating cyanobacteria of the present invention has the following advantages:
1. the method utilizes the microorganism denitrification and the biological strong-effect phosphorus-accumulating bacteria to balance the nitrogen-phosphorus ratio in the water body, controls the eutrophication state of the water body, controls the growth environment of the blue-green algae, controls the content of the nitrogen-phosphorus ratio not to be excessive, ensures that the growth of other organisms, plants and fishes is not influenced, utilizes the microorganism to inhibit the propagation and growth of the blue-green algae, avoids the outbreak growth of the blue-green algae, degrades the algal toxins generated after the blue-green algae die by adopting the microorganism, and finally uses fibers or quartz sand to precipitate and filter the residual impurities of the blue-green algae in the water body to avoid the secondary pollution generated after the blue-green algae die.
2. Adding high-activity catalyst for promoting ammoniation during ammoniation, and setting the adding amount of the high-activity catalyst to be 0.85g.L-1-1.25g.L-1The trace element additive is added into the phosphorus accumulating bacteria to raise the ammoniation and phosphorus accumulating efficiency, shorten the concentration time and lower the cost.
3. The sediment filtration of the residual impurities of the blue algae in the water body adopts quartz sand/fiber filtration, so that the water quality is further purified, and the water environment is ensured.
4. The biological treatment method of blue algae utilizes the life activities of microbes, plants and other organisms to absorb, degrade and remove water pollutants, thereby solving the eutrophication of water areas and purifying the water bodies. Creating environment suitable for various organisms to live and reproduce, and rebuilding and restoring water ecological system. Good biological treatment effect, no or low energy consumption, and low operation cost.
Detailed Description
The invention is further described below by means of specific embodiments.
A biological treatment method for blue algae sequentially comprises the following steps:
firstly, adopting microorganisms to control blue algae in a water body, specifically adopting biological nitrogen removal and biological phosphorus removal, wherein the biological nitrogen removal comprises ammoniation-nitrification-denitrification, the ammoniation is that ammoniation enzyme is adopted to carry out oxidative deamination under aerobic conditions, and in order to improve the ammoniation efficiency, a high-activity catalyst for promoting ammoniation is added in the ammoniation process, and the adding amount of the high-activity catalyst is 0.85g.L-1-1.25g.L-1The nitrifying step is to oxidize NH3-N into NxO-N by adopting nitrite bacteria and nitrate bacteria, and the denitrifying step is to reduce nitrate into nitrogen gas so as to reduce the content of nitrogen nutrition in the water body, eliminate the toxic action on organisms due to the accumulation of nitric acid, and remove phosphorus from the organisms under an aerobic or anoxic state;
the biological phosphorus removal means that the phosphorus-accumulating bacteria which are specifically cultured and enhanced by genes are utilized, the phosphorus-accumulating bacteria can absorb the phosphorus in the water body in an excessive manner in an aerobic or anoxic state to enable the phosphorus content in the water body to exceed a large number of strains of which the phosphorus content in the water body is several times that in the ordinary bacteria, and the enhanced phosphorus-accumulating bacteria can release the phosphorus stored in the water body under the anaerobic condition so as to obtain energy for other bacteria to maintain the survival needs of the bacteria in adverse environments, if the bacteria enter the aerobic environment with rich nutrition again, the in-vivo phosphorus accumulation process is repeated, preferably, a trace element additive is added into the phosphorus-accumulating bacteria, and the ratio of the phosphorus-accumulating bacteria to the trace element additive is 1: 1-5.
Another further point to be mentioned is that the species of phosphorus accumulating microorganisms in biological phosphorus removal mainly include Acinetobacter, Aeromonas and Pseudomonas, wherein the bacteria such as Acinetobacter can excessively take in phosphorus in sewage to form polyphosphate in cells, the content of the polyphosphate is 10% -20% of the dry weight of the cells, and the bacteria also have the capacity of accumulating fatty acid and poly beta-hydroxybutyrate, Aeromonas; aeromonas is also one of the main phosphorus-accumulating bacteria, and the bacteria account for 12-36 percent of the whole bacteria composition of a microbial system, although the Aeromonas can excessively absorb phosphorus in water to form a polyphosphate content; but its main role is to degrade organic matter. Under anaerobic conditions, metabolizing certain sugar and alcohol to generate short-chain volatile fatty acid; moreover, they can perform denitrification, for example, aeromonas hydrophila can reduce nitrate into nitrite, and other bacteria can directly reduce nitrate into nitrogen; pseudomonas, which is also an important bacterium in a biological phosphorus removal system, can accumulate polyphosphate with the content of 3l percent of the dry weight of the bacterium; under aerobic conditions, when the bacteria grow from logarithmic growth phase to stationary growth phase, the polyphosphate content also increases along with the prolonging of the culture time; the activity of phosphokinase is reduced along with the increase of polyphosphate, which shows that the polyphosphate has an inhibition effect on the polyphosphate kinase, nitrogen and phosphorus are necessary elements for the growth of organisms, if the content in water is excessive, a large amount of algae plants can grow and propagate, and the culture of other organisms is not facilitated, so that the blue algae is developed; therefore, by utilizing the advantages of biological denitrification and phosphorus accumulating bacteria, the nitrogen-phosphorus ratio content in the water body is controlled not to be excessive in one step, and the growth and the propagation of other organisms, plants and fishes are not influenced.
Step two: the microorganism is adopted for inhibiting, the microorganism is an algae dissolving bacterium, and the algae dissolving bacterium is directly or indirectly or synergistically inhibited to act on the blue algae:
direct action algae inhibition: the algicidal bacteria are directly adsorbed to the cyanobacteria cells, invade and parasitize the cyanobacteria cells, and finally cause the cyanobacteria cells to be damaged and die;
inhibiting algae by indirect action: the aim of inhibiting algae is achieved by utilizing the secretion and release of extracellular substances by algae-lysing bacteria or by the nutrition competition between bacteria and algae;
inhibiting algae under synergistic action: some algicidal bacteria have both direct and indirect effects, and the algae inhibition mode can be called as synergistic algae inhibition.
Step three: the microbial strains are adopted to degrade algal toxins produced after blue algae die, the microbial strains comprise sphingomonas, methylobacterium, lactobacillus strains, lactobacillus bifidus strains and lactobacillus plantarum strains, because algal toxins, neurotoxin and neurotoxin are produced after blue algae die, liver toxins cause liver pathological changes of cultured animals, and neurotoxin causes paralysis of cultured species and direct death. The microbial degrading bacteria have the characteristics of high degrading capacity to the algal toxin, easy culture and rapid growth, and can be used for treating the blue-green algae outbreak and controlling the growth and the propagation of the blue-green algae, so that the efficiency of degrading the algal toxin is greatly improved. The Sphingomonas is a rich novel microbial resource and can be used for biodegradation of aromatic compounds. The strain of the genus has high metabolic capability and multifunctional physiological characteristics,
in addition to sphingomonas, methylobacterium and (arthrobacter, brevibacterium and rhodococcus) and probiotic bacteria biodegrade microcystins: lactobacillus strain, Bifidobacterium lactis strain and Lactobacillus plantarum strain, wherein the final product degraded by the strains is CO2.(ii) a In another example, the pillared algal toxin is a cytotoxin produced by blue algae and is now listed as one of the important algal toxins in the world. The toxin has stable molecular structure, mainly inhibits the synthesis of protein and gluteptide glycomacropeptide due to toxicity, and has genotoxicity and potential carcinogenic property; the microorganism for degrading the Cytospora toxin is a bacillus strain, and the above microorganism degrading bacteria have high degrading capability to the Cytospora toxin, are easy to culture and growThe characteristic of rapid growth, which can greatly improve the efficiency of degrading the algal toxin for treating the blue algae outbreak and controlling the growth and the propagation of the blue algae.
Step four: the method is characterized in that the blue algae residual impurities in the water body are precipitated and filtered, and the impurities in the water are removed by quartz sand filtration or fiber filtration.
The biological treatment method of blue algae utilizes the life activities of microbes, plants and other organisms to absorb, degrade and remove water pollutants, thereby solving the eutrophication of water areas and purifying the water bodies. Creating environment suitable for various organisms to live and reproduce, and rebuilding and restoring water ecological system. Good biological treatment effect, no or low energy consumption, and low operation cost.
The above description is only a few specific embodiments of the present invention, but the design concept of the present invention is not limited thereto, and any insubstantial modifications made by the design concept should fall within the scope of the present invention.
Claims (10)
1. A blue algae biological treatment method is characterized in that: comprises the following steps in sequence
Step one, adopting microorganism control;
step two: adopting microorganism inhibition;
step three: degrading algal toxin generated after blue algae death by adopting a microbial strain;
step four: precipitating and filtering the residual impurities of the blue algae in the water body.
2. The cyanobacteria biological treatment method according to claim 1, characterized in that: biological nitrogen removal and biological phosphorus removal are sequentially adopted for biological control.
3. The cyanobacteria biological treatment method according to claim 2, characterized in that: the biological nitrogen removal comprises ammoniation-nitrification-denitrification, wherein ammoniation is to perform oxidative deamination by using ammoniation enzyme under an aerobic condition, the nitrification is to oxidize NH3-N into NxO-N by using nitrite bacteria and nitrate bacteria, the denitrification is to reduce nitrate into nitrogen so as to reduce the content of nitrogen nutrition in a water body and eliminate the toxic action on organisms due to nitric acid accumulation, and the biological phosphorus removal is performed under an aerobic or anoxic state.
4. The cyanobacteria biological treatment method according to claim 3, characterized in that: the ammoniation process is added with a high-activity catalyst for promoting ammoniation, and the adding amount of the high-activity catalyst is 0.85g.L-1-1.25g.L-1。
5. The cyanobacteria biological treatment method according to claim 1, characterized in that: the biological phosphorus removal is implemented by adopting phosphorus-accumulating bacteria which are specifically cultured and enhanced by genes, wherein a trace element additive is added into the phosphorus-accumulating bacteria, and the ratio of the phosphorus-accumulating bacteria to the trace element additive is 1: 1-5.
6. The cyanobacteria biological treatment method according to claim 1, characterized in that: the microorganism inhibition adopts algicidal bacteria, and the algicidal bacteria inhibit blue algae.
7. The cyanobacteria biological treatment method according to claim 6, characterized in that: the algicidal bacteria directly act or indirectly act or synergistically act on the blue algae.
8. The cyanobacteria biological treatment method according to claim 1, characterized in that: the bacterial strain comprises one or more of sphingomonas, methylobacterium, lactobacillus strain, double-finger lactobacillus strain and lactobacillus plantarum strain.
9. The cyanobacteria biological treatment method according to claim 1, characterized in that: the sediment filtration of the blue algae residual impurities in the water body is quartz sand filtration.
10. The cyanobacteria biological treatment method according to claim 1, characterized in that: and the precipitation and filtration of the residual impurities of the blue algae in the water body are fiber filtration.
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Application publication date: 20201124 |