CN111961002A - 吡唑醚菌酯半抗原、人工抗原和抗体及其制备方法和应用 - Google Patents
吡唑醚菌酯半抗原、人工抗原和抗体及其制备方法和应用 Download PDFInfo
- Publication number
- CN111961002A CN111961002A CN202010902312.7A CN202010902312A CN111961002A CN 111961002 A CN111961002 A CN 111961002A CN 202010902312 A CN202010902312 A CN 202010902312A CN 111961002 A CN111961002 A CN 111961002A
- Authority
- CN
- China
- Prior art keywords
- pyraclostrobin
- hapten
- reaction
- artificial antigen
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- HZRSNVGNWUDEFX-UHFFFAOYSA-N pyraclostrobin Chemical compound COC(=O)N(OC)C1=CC=CC=C1COC1=NN(C=2C=CC(Cl)=CC=2)C=C1 HZRSNVGNWUDEFX-UHFFFAOYSA-N 0.000 title claims abstract description 104
- 239000005869 Pyraclostrobin Substances 0.000 title claims abstract description 102
- 239000000427 antigen Substances 0.000 title claims abstract description 27
- 102000036639 antigens Human genes 0.000 title claims abstract description 27
- 108091007433 antigens Proteins 0.000 title claims abstract description 27
- 238000002360 preparation method Methods 0.000 title abstract description 13
- 238000001514 detection method Methods 0.000 claims abstract description 14
- 230000003053 immunization Effects 0.000 claims abstract description 12
- 102000014914 Carrier Proteins Human genes 0.000 claims abstract description 11
- 108010078791 Carrier Proteins Proteins 0.000 claims abstract description 11
- 241001465754 Metazoa Species 0.000 claims abstract description 10
- 230000008878 coupling Effects 0.000 claims abstract description 4
- 238000010168 coupling process Methods 0.000 claims abstract description 4
- 238000005859 coupling reaction Methods 0.000 claims abstract description 4
- 238000006243 chemical reaction Methods 0.000 claims description 30
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 claims description 27
- 238000000034 method Methods 0.000 claims description 20
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical group CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 229940098773 bovine serum albumin Drugs 0.000 claims description 9
- 238000002649 immunization Methods 0.000 claims description 9
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 8
- 239000002904 solvent Substances 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 7
- 239000012074 organic phase Substances 0.000 claims description 7
- 229940092253 ovalbumin Drugs 0.000 claims description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- 108010058846 Ovalbumin Proteins 0.000 claims description 6
- -1 4-chlorphenyl Chemical group 0.000 claims description 5
- SCRFKIWDJFFLKL-UHFFFAOYSA-N C(N)(OOC)=O.CC1=C(C=C(C=C1)I)CBr Chemical compound C(N)(OOC)=O.CC1=C(C=C(C=C1)I)CBr SCRFKIWDJFFLKL-UHFFFAOYSA-N 0.000 claims description 5
- KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 claims description 5
- 229940124277 aminobutyric acid Drugs 0.000 claims description 5
- 238000000605 extraction Methods 0.000 claims description 5
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 claims description 5
- 229910000104 sodium hydride Inorganic materials 0.000 claims description 5
- 239000012312 sodium hydride Substances 0.000 claims description 5
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N dimethyl sulfoxide Natural products CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 4
- 238000010534 nucleophilic substitution reaction Methods 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 238000001704 evaporation Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000011068 loading method Methods 0.000 claims description 3
- 239000012046 mixed solvent Substances 0.000 claims description 3
- 239000003208 petroleum Substances 0.000 claims description 3
- 238000002390 rotary evaporation Methods 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 108091006905 Human Serum Albumin Proteins 0.000 claims description 2
- 102000008100 Human Serum Albumin Human genes 0.000 claims description 2
- 238000010828 elution Methods 0.000 claims description 2
- 108060003552 hemocyanin Proteins 0.000 claims description 2
- 230000036046 immunoreaction Effects 0.000 claims description 2
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 230000035945 sensitivity Effects 0.000 abstract description 8
- 229930182692 Strobilurin Natural products 0.000 abstract description 5
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 4
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract description 4
- 238000003018 immunoassay Methods 0.000 abstract description 4
- 239000003899 bactericide agent Substances 0.000 abstract description 3
- 230000028993 immune response Effects 0.000 abstract description 3
- 230000005847 immunogenicity Effects 0.000 abstract description 3
- 230000000717 retained effect Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 21
- 241000699666 Mus <mouse, genus> Species 0.000 description 15
- 239000000243 solution Substances 0.000 description 13
- 238000002965 ELISA Methods 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 6
- 206010035226 Plasma cell myeloma Diseases 0.000 description 6
- 239000012980 RPMI-1640 medium Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 201000000050 myeloid neoplasm Diseases 0.000 description 6
- 239000006228 supernatant Substances 0.000 description 6
- 210000000683 abdominal cavity Anatomy 0.000 description 5
- 210000004989 spleen cell Anatomy 0.000 description 5
- 210000001015 abdomen Anatomy 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- UQLDLKMNUJERMK-UHFFFAOYSA-L di(octadecanoyloxy)lead Chemical compound [Pb+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O UQLDLKMNUJERMK-UHFFFAOYSA-L 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 230000002163 immunogen Effects 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 3
- 239000002671 adjuvant Substances 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 238000000576 coating method Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 210000004408 hybridoma Anatomy 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 210000004303 peritoneum Anatomy 0.000 description 3
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- FPQQSJJWHUJYPU-UHFFFAOYSA-N 3-(dimethylamino)propyliminomethylidene-ethylazanium;chloride Chemical compound Cl.CCN=C=NCCCN(C)C FPQQSJJWHUJYPU-UHFFFAOYSA-N 0.000 description 2
- 206010003445 Ascites Diseases 0.000 description 2
- 239000008346 aqueous phase Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000007910 cell fusion Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001804 emulsifying effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 239000000417 fungicide Substances 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 229920001223 polyethylene glycol Polymers 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- RYHBNJHYFVUHQT-UHFFFAOYSA-N 1,4-Dioxane Chemical compound C1COCCO1 RYHBNJHYFVUHQT-UHFFFAOYSA-N 0.000 description 1
- YOETUEMZNOLGDB-UHFFFAOYSA-N 2-methylpropyl carbonochloridate Chemical compound CC(C)COC(Cl)=O YOETUEMZNOLGDB-UHFFFAOYSA-N 0.000 description 1
- 241000235349 Ascomycota Species 0.000 description 1
- 239000005730 Azoxystrobin Substances 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 229910021595 Copper(I) iodide Inorganic materials 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000221785 Erysiphales Species 0.000 description 1
- 240000001624 Espostoa lanata Species 0.000 description 1
- 235000009161 Espostoa lanata Nutrition 0.000 description 1
- 239000005784 Fluoxastrobin Substances 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 239000005800 Kresoxim-methyl Substances 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 description 1
- 241000233654 Oomycetes Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000233679 Peronosporaceae Species 0.000 description 1
- 239000005818 Picoxystrobin Substances 0.000 description 1
- 229920001030 Polyethylene Glycol 4000 Polymers 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 241000918585 Pythium aphanidermatum Species 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000005857 Trifloxystrobin Substances 0.000 description 1
- MUUGCDGGIVCSDT-UHFFFAOYSA-N [NH4+].[NH4+].[O-]S([O-])(=O)=O.CCCCCCCC(O)=O Chemical compound [NH4+].[NH4+].[O-]S([O-])(=O)=O.CCCCCCCC(O)=O MUUGCDGGIVCSDT-UHFFFAOYSA-N 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 238000011091 antibody purification Methods 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- WFDXOXNFNRHQEC-GHRIWEEISA-N azoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 WFDXOXNFNRHQEC-GHRIWEEISA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- LSXDOTMGLUJQCM-UHFFFAOYSA-M copper(i) iodide Chemical compound I[Cu] LSXDOTMGLUJQCM-UHFFFAOYSA-M 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- UFEODZBUAFNAEU-NLRVBDNBSA-N fluoxastrobin Chemical compound C=1C=CC=C(OC=2C(=C(OC=3C(=CC=CC=3)Cl)N=CN=2)F)C=1C(=N/OC)\C1=NOCCO1 UFEODZBUAFNAEU-NLRVBDNBSA-N 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- ZOTBXTZVPHCKPN-HTXNQAPBSA-N kresoxim-methyl Chemical compound CO\N=C(\C(=O)OC)C1=CC=CC=C1COC1=CC=CC=C1C ZOTBXTZVPHCKPN-HTXNQAPBSA-N 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000001294 liquid chromatography-tandem mass spectrometry Methods 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- JHIPUJPTQJYEQK-ZLHHXESBSA-N orysastrobin Chemical compound CNC(=O)C(=N\OC)\C1=CC=CC=C1CO\N=C(/C)\C(=N\OC)\C(\C)=N\OC JHIPUJPTQJYEQK-ZLHHXESBSA-N 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- IBSNKSODLGJUMQ-SDNWHVSQSA-N picoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1COC1=CC=CC(C(F)(F)F)=N1 IBSNKSODLGJUMQ-SDNWHVSQSA-N 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 125000006850 spacer group Chemical group 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- ONCZDRURRATYFI-TVJDWZFNSA-N trifloxystrobin Chemical compound CO\N=C(\C(=O)OC)C1=CC=CC=C1CO\N=C(/C)C1=CC=CC(C(F)(F)F)=C1 ONCZDRURRATYFI-TVJDWZFNSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
- C07D231/02—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings
- C07D231/10—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members
- C07D231/14—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings not condensed with other rings having two or three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D231/18—One oxygen or sulfur atom
- C07D231/20—One oxygen atom attached in position 3 or 5
- C07D231/22—One oxygen atom attached in position 3 or 5 with aryl radicals attached to ring nitrogen atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/765—Serum albumin, e.g. HSA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/76—Albumins
- C07K14/77—Ovalbumin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/795—Porphyrin- or corrin-ring-containing peptides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/33—Crossreactivity, e.g. for species or epitope, or lack of said crossreactivity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
- C07K2317/35—Valency
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- Food Science & Technology (AREA)
- Toxicology (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010902312.7A CN111961002A (zh) | 2020-09-01 | 2020-09-01 | 吡唑醚菌酯半抗原、人工抗原和抗体及其制备方法和应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010902312.7A CN111961002A (zh) | 2020-09-01 | 2020-09-01 | 吡唑醚菌酯半抗原、人工抗原和抗体及其制备方法和应用 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN111961002A true CN111961002A (zh) | 2020-11-20 |
Family
ID=73400845
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202010902312.7A Pending CN111961002A (zh) | 2020-09-01 | 2020-09-01 | 吡唑醚菌酯半抗原、人工抗原和抗体及其制备方法和应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN111961002A (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112779225A (zh) * | 2021-03-17 | 2021-05-11 | 江南大学 | 一株分泌醚菌酯单克隆抗体杂交瘤细胞株及其应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106324240A (zh) * | 2016-08-04 | 2017-01-11 | 北京勤邦生物技术有限公司 | 检测毒死蜱的酶联免疫试剂盒及其应用 |
CN110642793A (zh) * | 2019-09-24 | 2020-01-03 | 北京勤邦生物技术有限公司 | 嘧菌酯半抗原、人工抗原和抗体及其制备方法和应用 |
CN111333570A (zh) * | 2020-03-05 | 2020-06-26 | 北京望尔生物技术有限公司 | 氟吡甲禾灵半抗原、人工抗原和抗体及其制备方法和应用 |
-
2020
- 2020-09-01 CN CN202010902312.7A patent/CN111961002A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106324240A (zh) * | 2016-08-04 | 2017-01-11 | 北京勤邦生物技术有限公司 | 检测毒死蜱的酶联免疫试剂盒及其应用 |
CN110642793A (zh) * | 2019-09-24 | 2020-01-03 | 北京勤邦生物技术有限公司 | 嘧菌酯半抗原、人工抗原和抗体及其制备方法和应用 |
CN111333570A (zh) * | 2020-03-05 | 2020-06-26 | 北京望尔生物技术有限公司 | 氟吡甲禾灵半抗原、人工抗原和抗体及其制备方法和应用 |
Non-Patent Citations (4)
Title |
---|
JOSEP V. MERCADER等: "Immunoassays for pyraclostrobin analysis in processed food products using novel monoclonal antibodies and QuEChERS-based extracts", 《FOOD CONTROL》, vol. 32, no. 1, pages 42 - 48 * |
JOSEP V. MERCADER等: "Production and Characterization of Monoclonal Antibodies Specific to the Strobilurin Pesticide Pyraclostrobin", 《J. AGRIC. FOOD CHEM.》, vol. 56, no. 17, pages 7682 - 7690 * |
JOSEP V. MERCADER等: "Synthesis of site-heterologous haptens for high-affinity anti-pyraclostrobin antibody generation", 《ORGANIC & BIOMOLECULAR CHEMISTRY》, vol. 9, pages 1443 - 1453 * |
戴立信: "Ullmann 反应百年纪念及近期复兴—兼及碳-杂原子键的形成", 《化学进展》, vol. 30, no. 9, pages 1257 - 1297 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112779225A (zh) * | 2021-03-17 | 2021-05-11 | 江南大学 | 一株分泌醚菌酯单克隆抗体杂交瘤细胞株及其应用 |
CN112779225B (zh) * | 2021-03-17 | 2022-10-18 | 江南大学 | 一株分泌醚菌酯单克隆抗体杂交瘤细胞株及其应用 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN111978304B (zh) | 苯醚甲环唑半抗原、人工抗原和抗体及其制备方法和应用 | |
CN110642793A (zh) | 嘧菌酯半抗原、人工抗原和抗体及其制备方法和应用 | |
CN110498766B (zh) | 氟啶胺半抗原、人工抗原和抗体及其制备方法和应用 | |
CN110845444B (zh) | 烯酰吗啉半抗原、人工抗原和抗体及其制备方法和应用 | |
CN111943882B (zh) | 腐霉利半抗原、人工抗原和抗体及其制备方法和应用 | |
CN111333570B (zh) | 氟吡甲禾灵半抗原、人工抗原和抗体及其制备方法和应用 | |
CN110627726B (zh) | 咪鲜胺半抗原、人工抗原和抗体及其制备方法和应用 | |
CN104356237A (zh) | 一种多效唑单克隆抗体的制备方法 | |
CN111269262B (zh) | 丙溴磷半抗原、人工抗原和抗体及其制备方法和应用 | |
CN110845429A (zh) | 戊唑醇半抗原、人工抗原和抗体及其制备方法和应用 | |
CN110879293A (zh) | 一种筛选分泌配对单克隆抗体的杂交瘤细胞株的方法与应用 | |
CN111333503A (zh) | 三氯杀螨醇半抗原、人工抗原和抗体及其制备方法和应用 | |
CN106918705B (zh) | 检测甲氰菊酯的试纸及其应用 | |
CN101885774B (zh) | 去氢甲睾酮单克隆抗体、制备方法及其应用 | |
CN113264834B (zh) | 一种冷杉醇半抗原、人工抗原和抗体及其制备方法和应用 | |
CN111961002A (zh) | 吡唑醚菌酯半抗原、人工抗原和抗体及其制备方法和应用 | |
CN113024415B (zh) | 氟氯氰菊酯半抗原、人工抗原和抗体及其制备方法和应用 | |
CN112480167B (zh) | 水胺硫磷半抗原、人工抗原和抗体及其制备方法和应用 | |
CN102924601B (zh) | 一种莱克多巴胺单克隆抗体的制备方法 | |
CN111875652B (zh) | 多杀菌素半抗原、人工抗原和抗体及其制备方法和应用 | |
CN110590561B (zh) | 除草醚半抗原、人工抗原和抗体及其制备方法和应用 | |
CN113896758B (zh) | 龙葵素半抗原、人工抗原和抗体及其制备方法和应用 | |
CN111943881B (zh) | 溴虫腈半抗原、人工抗原和抗体及其制备方法和应用 | |
CN113527390A (zh) | 泰拉霉素半抗原、人工抗原和抗体及其制备方法和应用 | |
CN111825566B (zh) | 六氯苯半抗原、人工抗原和抗体及其制备方法和应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information | ||
CB02 | Change of applicant information |
Address after: No.8, Gaoxin 4th Street, Huilongguan international information industry base, Changping District, Beijing Applicant after: Beijing Wanger Biotechnology Co.,Ltd. Applicant after: Beijing Qinbang Technology Co.,Ltd. Address before: No.8, Gaoxin 4th Street, Huilongguan international information industry base, Changping District, Beijing Applicant before: Beijing Wanger Biotechnology Co.,Ltd. Applicant before: BEIJING KWINBON BIOTECHNOLOGY Co.,Ltd. |
|
WD01 | Invention patent application deemed withdrawn after publication | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20201120 |