CN111960883A - Special bacterial fertilizer for soybean varieties and preparation method thereof - Google Patents
Special bacterial fertilizer for soybean varieties and preparation method thereof Download PDFInfo
- Publication number
- CN111960883A CN111960883A CN202010734755.XA CN202010734755A CN111960883A CN 111960883 A CN111960883 A CN 111960883A CN 202010734755 A CN202010734755 A CN 202010734755A CN 111960883 A CN111960883 A CN 111960883A
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- bacterial
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- soybean variety
- bacterial fertilizer
- vermiculite
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/90—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting the nitrification of ammonium compounds or urea in the soil
Abstract
The invention relates to the technical field of soybean planting, in particular to a special bacterial fertilizer for soybean varieties and a preparation method thereof, wherein the bacterial fertilizer has good yield-increasing effect and good adaptability, and the preparation method has simple operation method and low manufacturing cost; the antibacterial agent comprises grass peat, vermiculite and bacterial liquid, wherein the weight ratio of the grass peat to the vermiculite to the bacterial liquid is 2:2: 1.
Description
Technical Field
The invention relates to the technical field of soybean planting, in particular to a special bacterial fertilizer for soybean varieties and a preparation method thereof.
Background
As is known, the biological bacterial fertilizer is a novel environment-friendly fertilizer, beneficial microorganisms such as growth-promoting bacteria and the like contained in the biological bacterial fertilizer, namely plant rhizosphere growth-promoting bacteria, reduce the occurrence of plant diseases and insect pests, play a role in increasing the yield of plants, and also can improve the soil quality and prevent secondary pollution. The soybean is a leguminous crop and has the function of rhizobium symbiosis nitrogen fixation. According to field test, 1 mu of soybean can fix about 8kg of nitrogen, which is equivalent to applying 18kg of urea. However, all rhizobia cannot achieve the same inoculation effect for the same soybean variety, and the interaction between organisms indicates that the rhizobia and a host need to be well matched to achieve the optimal nitrogen fixation efficiency; different kinds of rhizobia exist in different soils, and the soil species in old areas also have different numbers of indigenous rhizobia, so that the symbiotic nitrogen fixation capacity of the rhizobia is stronger. Therefore, the problem that the most effective strain proportion is searched to adapt to different soil types and can be matched with a certain soybean variety is urgently needed to be solved at present. At present, no special bacterial manure for a certain soybean variety exists in the market.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide the special bacterial fertilizer for improving the yield of the soybeans and being suitable for a certain soybean variety.
The invention also aims to provide a preparation method of the special bacterial fertilizer for soybean varieties
The special bacterial fertilizer for the soybean variety comprises grass carbon, vermiculite and bacterial liquid, wherein the weight ratio of the grass carbon to the vermiculite to the bacterial liquid is 2:2: 1.
The special bacterial fertilizer for soybean varieties has effective viable count of more than 2.5 hundred million/g.
The special bacterial fertilizer for the soybean variety, disclosed by the invention, has the grass carbon and vermiculite sizes of less than 60 meshes.
The preparation method of the special bacterial fertilizer for the soybean variety comprises the following steps:
(1) obtaining strains: respectively planting a certain soybean variety in fertile soil with the soil organic matter content of 2.5-4% and barren saline-alkali soil with the soil organic matter content of 0.5-2.0% and the pH value of 7.9, respectively selecting field dominant plants after the soybean variety is mature, taking off root nodules at the root (part of the root), soaking the root nodules in 95% ethanol for 4min, sterilizing the surface of the soaked root nodules with 0.1% HgCl for 4min, and then cleaning the surface of the soaked root nodules with sterile water for 10 times for later use;
(2) culturing strains: the sterilized rhizobia prepared in the step (1) is crushed under the aseptic condition and is put into a culture medium for culture, after shaking culture is carried out for 60 hours at the constant temperature of pH7.0 and 30 ℃, a blood counting plate microscopy counting method is adopted in combination with a flat plate viable counting method, and the thallus density reaches 50 hundred million/ml for later use;
(3) preparing bacterial manure: mixing turf and vermiculite according to a weight ratio of 2:2 to serve as an adsorbent, screening and sterilizing through a 60-mesh sieve before adsorption, mixing bacterial liquid and the adsorbent according to a weight ratio of 1:4, adjusting the pH value to 7.0, fully and uniformly stirring, keeping the water content to be about 25%, fermenting at normal temperature for 3-5 days, and enabling the effective viable count to reach more than 2.5 hundred million/g to serve as a special bacterial fertilizer for a certain soybean variety.
The invention relates to a preparation method of a special bacterial fertilizer for soybean varieties, wherein the formula of a culture medium in the step (2) is as follows: 1.1g of bean cake flour, 0.6g of bran, 0.2g of MgSo 4.7H 2O, 0.6g of K2HPO4, 0.1g of NaCl and 0.5g of CaCl 2.6H 2O.
According to the preparation method of the special bacterial fertilizer for soybean varieties, in the step (3), a gamma-ray sterilization method is adopted for sterilization, and the irradiation dose is 200 Gy.
Compared with the prior art, the invention has the beneficial effects that: 1. the rhizobium has strong matching property with a host and good yield increasing effect, the strain of the bacterial fertilizer is naturally formed in different soil environments by the cango 10 soybean variety, the matching property with the cango 10 soybean variety is good, the optimal nitrogen fixation efficiency can be formed, and in 2017, compared with the experimental field of the academy of agriculture and forestry in Cangzhou, the yield is increased by 15 percent and 8 percent respectively by applying other bacterial fertilizers;
2. the bacterial manure has good adaptability, and the bacterial manure has wide strain sources, so the bacterial manure has good adaptability, not only can be suitable for fertile soil with high organic matter content, but also can be suitable for light saline-alkaline barren soil with low organic matter content.
3. The operation method is simple, the manufacturing cost is low, the operation method of the bacterial manure is simple, the culture of the strains can be completed in a small laboratory, the components of the adsorbent are turf and vermiculite, the cost is low, and the bacterial manure is suitable for being operated by family farms and planting cooperative.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
Examples
The special bacterial fertilizer for the soybean variety comprises grass carbon, vermiculite and bacterial liquid, wherein the weight ratio of the grass carbon to the vermiculite to the bacterial liquid is 2:2: 1.
The special bacterial fertilizer for soybean varieties has effective viable count of more than 2.5 hundred million/g.
The special bacterial fertilizer for the soybean variety, disclosed by the invention, has the grass carbon and vermiculite sizes of less than 60 meshes.
The preparation method of the special bacterial fertilizer for the soybean variety comprises the following steps:
(1) obtaining strains: respectively planting a certain soybean variety in fertile soil with the soil organic matter content of 2.5-4% and barren saline-alkali soil with the soil organic matter content of 0.5-2.0% and the pH value of 7.9, respectively selecting field dominant plants after the soybean variety is mature, taking off root nodules at the root (part of the root), soaking the root nodules in 95% ethanol for 4min, sterilizing the surface of the soaked root nodules with 0.1% HgCl for 4min, and then cleaning the surface of the soaked root nodules with sterile water for 10 times for later use;
(2) culturing strains: the sterilized rhizobia prepared in the step (1) is crushed under the aseptic condition and is put into a culture medium (the formula of the culture medium is 1.1g of bean cake powder, 0.6g of bran, 0.2g of MgSo 4.7H 2O, 0.6g of K2HPO4, 0.1g of NaCl and 0.5g of CaCl 2.6H 2O) for culture, after shaking culture at constant temperature of pH7.0 and 30 ℃ for 60 hours, a blood counting plate microscopic examination counting method is adopted to combine with a flat plate viable bacteria counting method, and the thallus density reaches 50 billion per ml for later use;
(3) preparing bacterial manure: mixing turf and vermiculite according to a weight ratio of 2:2 to serve as an adsorbent, screening and sterilizing through a 60-mesh sieve before adsorption (gamma ray sterilization method is adopted for sterilization, and the irradiation dose is 200Gy), mixing bacterial liquid and the adsorbent according to a weight ratio of 1:4, adjusting the pH value to 7.0, fully and uniformly stirring, keeping the water content at about 25%, and after fermenting for 3-5 days at normal temperature, enabling the effective viable count to reach more than 2.5 hundred million/g, so that the special bacterial fertilizer for a certain soybean variety can be obtained.
The effect of the bacterial manure is tested in 2017 in academy of agriculture and forestry in Cangzhou, the test is carried out at a former test station of academy of agriculture and forestry in Cangzhou, the area of a cell is 50 square meters, three times of repetition are set, three processes are repeatedly set every time, and the three processes are respectively as follows: the bacterial manure, the bacterial manure and the Heilong soybean bacterial manure are not applied, the application modes are seed dressing, the planted soybean variety is cang bean 10, the organic matter content in the soil is 1.9 percent, the ph value is 7.1, the sowing time is 6 months and 15 days, the field management is the same as that in the field,
the following table shows a comparison of the yields of the three treatment regimes
The results prove that: the bacterial manure can be applied to increase the yield by 15 percent and 8 percent respectively compared with the bacterial manure and other bacterial manure.
The above description is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be regarded as the protection scope of the present invention.
Claims (6)
1. The special bacterial fertilizer for the soybean variety is characterized by comprising grass carbon, vermiculite and bacterial liquid, wherein the weight ratio of the grass carbon to the vermiculite to the bacterial liquid is 2:2: 1.
2. The bacterial fertilizer special for soybean varieties as claimed in claim 1, wherein the effective viable count is more than 2.5 hundred million/g.
3. The bacterial fertilizer special for soybean varieties as claimed in claim 2, wherein the sizes of the grass peat and the vermiculite are less than 60 meshes.
4. The method for preparing the bacterial fertilizer special for the soybean variety as claimed in any one of claims 1 to 3, comprising the following steps:
(1) obtaining strains: respectively planting a certain soybean variety in fertile soil with the soil organic matter content of 2.5-4% and barren saline-alkali soil with the soil organic matter content of 0.5-2.0% and the pH value of 7.9, respectively selecting field dominant plants after the soybean variety is mature, taking off root nodules at the root (part of the root), soaking the root nodules in 95% ethanol for 4min, sterilizing the surface of the soaked root nodules with 0.1% HgCl for 4min, and then cleaning the surface of the soaked root nodules with sterile water for 10 times for later use;
(2) culturing strains: the sterilized rhizobia prepared in the step (1) is crushed under the aseptic condition and is put into a culture medium for culture, after shaking culture is carried out for 60 hours at the constant temperature of pH7.0 and 30 ℃, a blood counting plate microscopy counting method is adopted in combination with a flat plate viable counting method, and the thallus density reaches 50 hundred million/ml for later use;
(3) preparing bacterial manure: mixing turf and vermiculite according to a weight ratio of 2:2 to serve as an adsorbent, screening and sterilizing through a 60-mesh sieve before adsorption, mixing bacterial liquid and the adsorbent according to a weight ratio of 1:4, adjusting the pH value to 7.0, fully and uniformly stirring, keeping the water content to be about 25%, fermenting at normal temperature for 3-5 days, and enabling the effective viable count to reach more than 2.5 hundred million/g to serve as a special bacterial fertilizer for a certain soybean variety.
5. The method for preparing the bacterial fertilizer special for the soybean variety as claimed in claim 4, wherein the formula of the culture medium in the step (2) is as follows: 1.1g of bean cake flour, 0.6g of bran, 0.2g of MgSo 4.7H 2O, 0.6g of K2HPO4, 0.1g of NaCl and 0.5g of CaCl 2.6H 2O.
6. The method for preparing bacterial manure special for soybean varieties according to claim 5, wherein the sterilization in the step (3) adopts a gamma-ray sterilization method, and the irradiation dose is 200 Gy.
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| CN202010734755.XA CN111960883A (en) | 2020-07-28 | 2020-07-28 | Special bacterial fertilizer for soybean varieties and preparation method thereof |
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| CN202010734755.XA CN111960883A (en) | 2020-07-28 | 2020-07-28 | Special bacterial fertilizer for soybean varieties and preparation method thereof |
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Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1083458A (en) * | 1993-10-12 | 1994-03-09 | 杨爱民 | A kind of production method of azotobacteria fertilizer |
| CN1156179A (en) * | 1996-12-18 | 1997-08-06 | 张龙男 | High-efficiency root-nodule bacteria for increasing yield of leguminous plant |
| CN1191854A (en) * | 1998-02-25 | 1998-09-02 | 北京乐泰然经济技术咨询有限责任公司 | Composite microbial fertilizer and its production process |
| CN1789214A (en) * | 2004-12-17 | 2006-06-21 | 中国科学院沈阳应用生态研究所 | Biological formulation for soybean and its production method |
| CN101519644A (en) * | 2009-04-03 | 2009-09-02 | 中国农业大学 | Sinorhizobium sp. and application thereof |
| CN101585736A (en) * | 2009-07-03 | 2009-11-25 | 嵇书琼 | A kind of agricultural complex micro organism fungicide and preparation method thereof |
| CN102503735A (en) * | 2011-11-02 | 2012-06-20 | 山东金正大生态工程股份有限公司 | Special rhizobium-containing controlled release fertilizer for soybean as well as preparation and application thereof |
| CN105087414A (en) * | 2014-05-19 | 2015-11-25 | 段一皋 | High-concentration compound rhizobium inoculant for symbiotic nitrogen fixation and preparation method thereof |
| CN105367221A (en) * | 2015-11-06 | 2016-03-02 | 南宁隆盛农业科技有限公司 | Organic fertilizer |
| CN105801209A (en) * | 2014-08-19 | 2016-07-27 | 杨献美 | Method for preparing special humic acid-urea coated rhizobium fertilizer for soybeans |
| CN107778105A (en) * | 2016-08-30 | 2018-03-09 | 康平绿友生物肥料有限公司 | A kind of multi-strain bio-organic fertilizer and preparation method thereof |
| CN108101712A (en) * | 2018-02-06 | 2018-06-01 | 安徽辉隆集团五禾生态肥业有限公司 | Dedicated fertilizer for soybean and preparation method containing rhizobium |
| CN108676722A (en) * | 2018-07-13 | 2018-10-19 | 北京四纬生物科技有限公司 | Improve the store method of nitragin survival rate |
| CN109735468A (en) * | 2019-02-14 | 2019-05-10 | 黑龙江省科学院微生物研究所 | One plant with wide spectrum dross characteristic soybean slowly raw rhizobium and its application and with its preparation compound nitragin |
| CN109880759A (en) * | 2019-03-06 | 2019-06-14 | 黑龙江省科学院微生物研究所 | A kind of rihizobium japonicum for being suitble to that freeze dried powder is made and its application |
-
2020
- 2020-07-28 CN CN202010734755.XA patent/CN111960883A/en active Pending
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1083458A (en) * | 1993-10-12 | 1994-03-09 | 杨爱民 | A kind of production method of azotobacteria fertilizer |
| CN1156179A (en) * | 1996-12-18 | 1997-08-06 | 张龙男 | High-efficiency root-nodule bacteria for increasing yield of leguminous plant |
| CN1191854A (en) * | 1998-02-25 | 1998-09-02 | 北京乐泰然经济技术咨询有限责任公司 | Composite microbial fertilizer and its production process |
| CN1789214A (en) * | 2004-12-17 | 2006-06-21 | 中国科学院沈阳应用生态研究所 | Biological formulation for soybean and its production method |
| CN101519644A (en) * | 2009-04-03 | 2009-09-02 | 中国农业大学 | Sinorhizobium sp. and application thereof |
| CN101585736A (en) * | 2009-07-03 | 2009-11-25 | 嵇书琼 | A kind of agricultural complex micro organism fungicide and preparation method thereof |
| CN102503735A (en) * | 2011-11-02 | 2012-06-20 | 山东金正大生态工程股份有限公司 | Special rhizobium-containing controlled release fertilizer for soybean as well as preparation and application thereof |
| CN105087414A (en) * | 2014-05-19 | 2015-11-25 | 段一皋 | High-concentration compound rhizobium inoculant for symbiotic nitrogen fixation and preparation method thereof |
| CN105801209A (en) * | 2014-08-19 | 2016-07-27 | 杨献美 | Method for preparing special humic acid-urea coated rhizobium fertilizer for soybeans |
| CN105367221A (en) * | 2015-11-06 | 2016-03-02 | 南宁隆盛农业科技有限公司 | Organic fertilizer |
| CN107778105A (en) * | 2016-08-30 | 2018-03-09 | 康平绿友生物肥料有限公司 | A kind of multi-strain bio-organic fertilizer and preparation method thereof |
| CN108101712A (en) * | 2018-02-06 | 2018-06-01 | 安徽辉隆集团五禾生态肥业有限公司 | Dedicated fertilizer for soybean and preparation method containing rhizobium |
| CN108676722A (en) * | 2018-07-13 | 2018-10-19 | 北京四纬生物科技有限公司 | Improve the store method of nitragin survival rate |
| CN109735468A (en) * | 2019-02-14 | 2019-05-10 | 黑龙江省科学院微生物研究所 | One plant with wide spectrum dross characteristic soybean slowly raw rhizobium and its application and with its preparation compound nitragin |
| CN109880759A (en) * | 2019-03-06 | 2019-06-14 | 黑龙江省科学院微生物研究所 | A kind of rihizobium japonicum for being suitble to that freeze dried powder is made and its application |
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Application publication date: 20201120 |