CN111956684A - Anti-aging composition and preparation method thereof - Google Patents

Abstract

The invention discloses an anti-aging composition and a preparation method thereof, and relates to the technical field of health products and medicines. The anti-aging composition comprises main effective components and a synergist, wherein the main effective components are beta-nicotinamide mononucleotide, rhodiola rosea and kudzu root, and the synergist is at least one of apple pectin, basil extract, lecithin and abelmoschus esculentus extract. The composition of the invention is a combination of various effective components and a synergist, and the anti-aging effect of the main effective components is obviously improved. The pharmaceutical composition has good curative effect, can improve metabolic activity and control cholesterol content, and has simple process, short preparation time and good application prospect.

Description

Anti-aging composition and preparation method thereof

Technical Field

The invention relates to the technical field of health products and medicines, in particular to an anti-aging composition and a preparation method thereof.

Background

Aging is an unaliatable natural law, and with the increase of age, the metabolism in vivo and the functions of each organ gradually decline, and the signs of aging of the body appearance and internal organs become more obvious. Aging includes signs of morphological aging, signs of visceral aging, and signs of functional aging. In ancient times and in modern times, people are looking for various methods for prolonging life and anti-aging drugs, so as to delay aging or improve life quality within a life limit defined in genetics. The market share of the American anti-aging products in the health products and the medicines at present reaches 33 percent and 20 percent respectively, and the market share of the Chinese anti-aging products in the health products and the medicines also reaches about 5 percent. China has already stepped into aging countries, the demand for anti-aging is more and more, and the growth speed of the anti-aging health products is also rapidly increased, which means that the anti-aging health products have huge market demand and have very broad development prospects for the research of the anti-aging health products.

Related reports can be seen on the anti-aging health care product/medicine composition, common anti-aging traditional Chinese medicines such as ginseng, astragalus, medlar, schisandra chinensis, lucid ganoderma, pseudo-ginseng and acanthopanax are mostly used for composing the anti-aging health care product/medicine composition, or active ingredients extracted from chemical medicines and natural products with the function of delaying aging are supplemented for composing the anti-aging health care product/medicine composition, and the common problems of too many composing medicines, large dosage and unobvious anti-aging effect exist.

Chinese patent application 201610158151.9 discloses an anti-aging traditional Chinese medicine composition, which comprises 7-9g of rhodiola rosea, 10-15g of acanthopanax, 10-15g of American ginseng, 16-23g of gynostemma pentaphylla, 10-15g of saffron, 6-8g of Zihedong, 12-16g of lucid ganoderma, 10-16g of common yam rhizome, 9-14g of dwarf lilyturf tuber, 10-14g of dense yellow stilbene, 14-16g of divaricate saposhnikovia root, 12-15g of red sage root, 10-15g of tall gastrodia tuber, 10-14g of Chinese angelica, 8-13g of longan pulp, 10-18g of pawpaw, 6-10g of eucommia bark, 8-11g of himalayan teasel root, 9-15g of lily, 6-10g of grassleaf sweelflag rhizome, 7-12g of obscured homalomena rhizome, 10-13 g. The composition is composed of pure traditional Chinese medicinal materials, does not contain chemical components or hormone, but has a complex formula, the quality control of the traditional Chinese medicinal materials is difficult, the formula has slow effect, a good anti-aging effect is realized, the composition is required to be taken for a long time, and the dosage is too large.

Chinese patent application 202010343066.6 discloses an anti-aging composition, which comprises main effective components and a synergist, wherein the main effective components are beta-nicotinamide mononucleotide, American ginseng, tall gastrodia tuber, rhodiola rosea, kudzuvine root and ginkgo leaf extract, and the synergist is one or more of zinc sulfate, chitosan, lecithin and basil extract. The composition disclosed by the invention is a combination of multiple effective components and a synergist, and the anti-aging effect of the composition is more obvious than that of a composition containing the effective components alone; the preparation process of the invention combines ozone and superfine grinding, and the obtained pharmaceutical composition has good anti-aging curative effect.

In order to further improve the anti-aging effect and reduce the complexity and safety risk of a medicine formula, the invention provides the anti-aging composition and the preparation method thereof.

Disclosure of Invention

The invention provides an anti-aging composition and a preparation method thereof, the anti-aging composition prepared by the invention is characterized in that on the basis of adding effective anti-aging medicinal materials, at least one of an abelmoschus manihot extract, apple pectin, lecithin and a basil extract is particularly added as a synergist, so that the absorption effect of a human body is remarkably improved, the dosage is low, and the drug effect is good; the ultramicro wall-breaking pulverization is adopted in the preparation process, so that the effective ingredients are easy to release, and the preparation time is saved.

The technical scheme of the invention is as follows:

an antiaging composition comprises main effective components and synergist; the main effective components comprise beta-nicotinamide mononucleotide, rhodiola rosea and kudzu-vine root; the synergist is at least one of Abelmoschus manihot extract, apple pectin, lecithin and basil extract. The weight ratio of the main effective components to the synergist is 8.5-21:1.74-2.76, preferably 12.25: 2.

The rhodiola rosea and the kudzuvine root can be medicinal materials or extracts, and the medicinal materials are adopted in the embodiment of the invention.

Wherein the content of the first and second substances,

the main effective components comprise 7 to 15 parts of beta-nicotinamide mononucleotide, 0.5 to 2 parts of rhodiola rosea and 1 to 4 parts of kudzu root by weight; preferably, the main effective components comprise 9.25 parts of beta-nicotinamide mononucleotide, 1 part of rhodiola rosea and 2 parts of kudzu root according to parts by weight.

The synergist is preferably an abelmoschus manihot extract, apple pectin, lecithin and basil extract, and further preferably comprises 0.1-0.2 part of the abelmoschus manihot extract, 0.1-0.2 part of the apple pectin, 0.2-0.5 part of the lecithin and 1-3 parts of the basil extract in parts by weight; more preferably, the weight ratio of the abelmoschus manihot extract to the apple pectin to the lecithin to the basil extract is 3:3:4: 30; most preferably, the synergist comprises 0.15 parts of abelmoschus esculentus extract, 0.15 parts of apple pectin, 0.2 parts of lecithin and 1.5 parts of basil extract by weight.

The main effective component also comprises nicotinamide.

Preferably, the weight part of the nicotinamide is 1-3 parts, and more preferably 1.5 parts.

The anti-aging composition can be prepared into dosage forms including powder, capsules, tablets, granules and pills.

In addition, the invention also provides a preparation method of the anti-aging composition, which comprises the following steps:

(1) cleaning the rhodiola rosea and the kudzuvine root according to the formula dosage, soaking in water, centrifuging and quickly freezing;

(2) micronizing frozen radix Rhodiolae and radix Puerariae, oven drying, and sieving to obtain mixture 1;

(3) adding beta-nicotinamide mononucleotide, nicotinamide and a synergist with formula dosage into the mixture 1 obtained in the step (2), and uniformly mixing to obtain the anti-aging composition.

Wherein the content of the first and second substances,

in the step (1), the water is soaked for 20-40min at the temperature of 25-35 ℃; preferably, the soaking time is 30min, and the temperature is 30 ℃.

In the step (1), the rotation speed of the centrifugation is 5000-; preferably at 6000rpm for 10 min.

In the step (1), the rapid freezing refers to that the centrifuged medicinal materials are put into an ultra-low temperature refrigerator with the temperature of 70 ℃ below zero for freezing for 20 to 50 min.

In the step (2), the superfine grinding time is 20-30min, preferably 25 min.

In the step (2), the drying refers to drying at 50-80 ℃.

In the step (3), the sieving refers to sieving with a sieve of 400-600 meshes.

The invention has the following beneficial effects:

(1) at least one of the abelmoschus manihot extract, the apple pectin, the lecithin and the basil extract is added as a synergist, and the dosage and the proportion of the synergist are determined, so that the main effective component composition is reduced, the safety is improved, and the cost is reduced;

(2) the anti-aging composition can obviously prolong the service life of a mouse, improve the metabolic activity, stabilize the change of cholesterol in the serum of the mouse and have good anti-aging curative effect;

(3) the preparation method of superfine grinding after freezing is adopted, the process is simple and easy to operate, good grinding effect is achieved, the absorption of effective ingredients by organisms is facilitated, and the bioavailability of the medicinal materials is improved.

Detailed Description

The present invention will be further explained with reference to specific examples in order to make the technical means, the technical features, the technical objectives and the effects of the present invention easier to understand, but the following examples are only preferred embodiments of the present invention, and not all embodiments of the present invention. Based on the embodiments in the implementation, other embodiments obtained by those skilled in the art without any creative efforts belong to the protection scope of the present invention.

In the following examples, unless otherwise specified, all the procedures and equipment used were conventional procedures and equipment used was conventional equipment.

The ultrafine grinder used in the invention is a model ZYJ-400 ultrafine grinder of Haoxin pharmaceutical machinery manufacturing Limited of Jiangyin.

The basil extract used in the invention is purchased from Shanxi Xintianyu Biotechnology GmbH, with the product number of XTY 191024; the extract of abelmoschus manihot used in the present invention is purchased from New Zealand Biotechnology Ltd under the trade name TY 181205.

Examples 1 to 12

The formulations of examples 1 to 12 are shown in tables 1 and 2.

Table 1.

Table 2.

Examples 1-12 were all prepared as follows:

(1) cleaning radix Rhodiolae and radix Puerariae, soaking in 30 deg.C water for 30min, centrifuging at 6000rpm for 10min, and freezing at-70 deg.C for 30 min;

(2) micronizing frozen radix Rhodiolae and radix Puerariae for 25min, oven drying at 65 deg.C, and sieving with 500 mesh sieve to obtain mixture 1;

(3) adding beta-nicotinamide mononucleotide, nicotinamide and a synergist with formula dosage into the mixture 1 obtained in the step (2), and uniformly mixing to obtain the anti-aging composition.

Comparative examples 1 to 7

The formulations of comparative examples 1-7 are shown in Table 3, prepared in the same manner as in example 1:

table 3.

Comparative example 8

Unlike example 1, comparative example 8 was prepared as follows, with the remainder being the same:

(1) cleaning radix Rhodiolae and radix Puerariae, oven drying at 65 deg.C, micronizing for 25min, and sieving with 500 mesh sieve to obtain mixture 1;

(2) adding beta-nicotinamide mononucleotide, nicotinamide and a synergist with formula dosage into the mixture 1 obtained in the step (1), and uniformly mixing to obtain the anti-aging composition.

Comparative example 9

In contrast to example 1, apple pectin was replaced with an equal amount of hawthorn pectin, and the rest was the same.

Result detection

1. Appearance and yield

Observing appearance, and counting sieving yield, as shown in Table 4;

the sieving yield is mass after sieving/mass before sieving × 100%.

Table 4.

Group of	Yield of the product	Colour(s)
			Examples 1 to 12	>95％	Light brown
Comparative examples 1 to 7 and 9	>95％	Light brown
			Comparative example 8	72％	Light brown

As can be seen from Table 4, the present invention achieves higher yields when subjected to ultramicro-processing after freezing.

2. Mouse longevity statistics

The method comprises the steps of taking rapid aging model mice SAM-P/8(3 months old), dividing male and female halves into 22 groups at random, carrying out adaptive breeding on 20 mice in each group in an animal room for 2 weeks, and adjusting illumination according to the day rhythm of 12 hours. During the breeding period, the mice can freely enter water and eat food, the temperature of the breeding environment is always kept between 20 and 22 ℃, and the relative humidity is 50 to 60 percent. After 2 weeks of adaptation period, the experiment group mice were separately administered with 100mg/kg of drug administration by gavage once a day, the blank group was gavage with physiological saline of the same amount, and the average life of each group of mice was counted after 9 months of feeding. The results are shown in Table 5:

TABLE 5 mean Life time of groups of mice

As can be seen from the data in Table 7, the lifespan of the mice was significantly improved after the anti-aging composition of the present invention was continuously administered, indicating that the anti-aging effect of the present invention was good. Among them, example 12 is the most preferable example, and the average lifespan of the mouse is 311.7 days. It can be seen from comparative examples 3-7 that in the absence of the synergist components, the anti-aging effect was not significant, indicating that there was a synergistic effect between the synergist components, and that the effect was best when the weight ratio of the abelmoschus manihot extract, apple pectin, lecithin and basil extract was 3:3:4: 40. Comparative example 8 proves that the preparation method of the present invention better improves the bioavailability of the main effective ingredients, and further improves the drug effect.

3. Metabolic analysis

After the mice in the above experiment were continuously bred for 6 months, the mice of example 1, example 12, comparative example 7, comparative example 8 and blank group were extracted, and metabolic parameters were measured using the Oxymax indirect calorimetry system (Columbus instruments, Columbus, OH). Before data measurement, mice were individually acclimated for 24 hours at a temperature of 22-24 ℃ under a 12 hour light/12 hour dark cycle. Oxymax System VO₂And VCO₂The ratios are set as follows: air flow 0.7 liter/min; the sample flow rate is 0.6 liter/min; the correction time is 5 min; the measurement time was 4 min. The system is calibrated according to a standard gas mixture and the consumed O is measured₂(VO₂Ml/kg/hr) and CO produced₂(VCO₂Ml/kg/hr). Evaluation of oxygen consumption (VO) over 24 hours₂) And respiratory entropy (VCO)₂/VO₂In accordance with O) and then₂Resulting heat value (3.815+1.232x entropy of respiration) and O₂Volumetric consumed calculation energy consumption (EE):

TABLE 6 oxygen consumption measurement (ml/g/hr)

Type of administration	3h	13h	23h
				Example 1	55.7±3.04*	52.8±5.11*	49.6±3.68*
Example 12	58.9±4.87*	56.6±3.45*	53.1±4.46*
				Comparative example 7	50.6±5.16*	48.5±5.46*	44.2±3.23
Comparative example 8	52.1±6.01*	50.3±6.33*	47.2±4.15*
				Blank group	49.2±6.34	47.6±6.57	43.7±5.86

Note that represents p <0.05 compared to the blank group

TABLE 7 energy consumption measurement results (KJ/hr)

Type of administration	3h	13h	23h
				Example 1	30.1±2.05*	27.4±3.39*	23.5±1.96*
Example 12	32.3±3.02*	28.5±4.31*	24.8±3.66*
				Comparative example 7	24.3±3.54*	23.1±2.83*	19.7±2.68*
Comparative example 8	28.4±2.25*	24.9±3.11*	21.0±1.79*
				Blank group	23.6±2.31	22.9±2.67	18.4±1.64

Note that represents p <0.05 compared to the blank group

As can be seen from tables 6 and 7, the oxygen consumption and energy consumption of the mice in the examples are significantly improved compared to the blank group, and the anti-aging composition provided by the invention is proved to be capable of improving the metabolic activity of the mice and preventing the energy metabolism injury related to the age of the mice to a certain extent, so as to prevent aging.

4. Cholesterol level detection

Healthy three-month-old mice are taken, the weight of the mice is 18-22g, the mice are randomly divided into 5 groups, 20 mice are taken in each group, and the mice are fed with high-fat feed in half of males and females. The compositions of example 1, example 12, comparative example 7 and comparative example 8 were administered at a dose of 100mg/kg by gavage once a day, and the blank group was gavage with an equal amount of physiological saline, and the mice in each group were allowed to eat and drink water freely, and the total cholesterol content in serum was measured in mice of 0 day, 30 days and 60 days, respectively. The results are shown in Table 8:

TABLE 8 Total Cholesterol levels (mmol/L) at different time periods for different groups of mice

Type of administration	Day 0	30 days	60 days
				Example 1	1.42±0.18	1.60±0.17*	1.71±0.25*
Example 12	1.43±0.27	1.54±0.11*	1.64±0.23*
				Comparative example 7	1.42±0.24	1.92±0.16*	2.40±0.25*
Comparative example 8	1.43±0.19	1.67±0.16*	1.78±0.18*
				Blank group	1.42±0.22	1.94±0.18	2.51±0.21

Note that represents p <0.05 compared to the blank group

It can be seen that the present invention can stabilize the cholesterol level in the serum of mice and control the increase of cholesterol in mice, and compared with the comparative examples, examples 1 and 12 can achieve the obvious effect of controlling the increase of cholesterol.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (10)

1. An anti-aging composition, which is characterized by comprising main effective components and a synergist; the main effective components comprise beta-nicotinamide mononucleotide, rhodiola rosea and kudzu-vine root; the synergist is at least one of Abelmoschus manihot extract, apple pectin, lecithin and basil extract.

2. The anti-aging composition according to claim 1, wherein the weight ratio of the main effective ingredient to the synergist is 8.5-21: 1.74-2.76.

3. The anti-aging composition according to claim 1, wherein the main effective ingredients comprise 7 to 15 parts by weight of beta-nicotinamide mononucleotide, 0.5 to 2 parts by weight of rhodiola rosea and 1 to 4 parts by weight of kudzu root.

4. The anti-aging composition according to claim 1, wherein the synergist comprises, by weight, 0.1 to 0.2 parts of abelmoschus manihot extract, 0.1 to 0.2 parts of apple pectin, 0.2 to 0.5 parts of lecithin, and 1 to 3 parts of basil extract.

5. The anti-aging composition according to claim 4, wherein the weight ratio of the abelmoschus manihot extract, the apple pectin, the lecithin and the basil extract is 3:3:4: 30.

6. The anti-aging composition according to any one of claims 1 to 3, wherein the main active ingredient further comprises niacinamide.

7. The anti-aging composition according to claim 6, wherein the nicotinamide is present in an amount of 1-3 parts by weight.

8. The anti-aging composition of any one of claims 1 to 7, in the form of a dosage form comprising powders, capsules, tablets, granules and pills.

9. The method for preparing the antiaging composition according to any one of claims 1 to 7, characterized by comprising the steps of:

(1) cleaning the rhodiola rosea and the kudzuvine root according to the formula dosage, soaking in water, centrifuging and quickly freezing;

(2) micronizing frozen radix Rhodiolae and radix Puerariae, oven drying, and sieving to obtain mixture 1;

(3) adding beta-nicotinamide mononucleotide, nicotinamide and a synergist with formula dosage into the mixture 1 obtained in the step (2), and uniformly mixing to obtain the anti-aging composition.

10. The method according to claim 9, wherein the micronization time in the step (2) is 20-30 min.