CN111939595A

CN111939595A - Chromatographic column device, equipment and method

Info

Publication number: CN111939595A
Application number: CN202010788734.6A
Authority: CN
Inventors: 黄超囡; 马继平
Original assignee: Qingdao University of Technology
Current assignee: Qingdao University of Technology
Priority date: 2020-08-07
Filing date: 2020-08-07
Publication date: 2020-11-17

Abstract

The invention discloses a chromatographic column device, equipment and a method, and the technical scheme is as follows: the bottle opening connecting column comprises a column tube, wherein one end of the column tube is connected with a column neck, the other end of the column tube is connected with a dropping liquid tube, and a bottle opening connecting column is arranged on the outer side of the dropping liquid tube; wherein, the pillar pipe is inside to have the back taper cavity, and the back taper cavity sets up the sieve with the link of dropping liquid pipe. The invention increases the number of chromatographic column plates through the conical structure in the column tube, and improves the chromatographic resolution; the exhaust tube can link to each other with the vacuum pump, increases dropping liquid speed, improves separation analysis efficiency.

Description

Chromatographic column device, equipment and method

Technical Field

The invention relates to the field of chemical separation and purification, in particular to a chromatographic column device, chromatographic column equipment and a chromatographic column method.

Background

The chromatographic column is common equipment in the field of chemical separation and purification, a column tube of the common chromatographic column is of a cylindrical structure, and a solution passes through an adsorption medium under the action of gravity, so that on one hand, a better separation effect can be obtained only by filling more fillers, and the experiment cost is increased; on the other hand, the separation speed is limited by the conditions of the granularity of the adsorption medium, the filling compactness, the solution viscosity and the like, and the separation speed is slow and the working efficiency is low due to no decompression.

Disclosure of Invention

Aiming at the defects in the prior art, the invention aims to provide a chromatographic column device, equipment and a method, wherein the number of chromatographic column plates is increased through a conical structure in a column tube, so that the chromatographic separation degree is improved; the exhaust tube can link to each other with the vacuum pump, increases dropping liquid speed, improves separation analysis efficiency.

In order to achieve the purpose, the invention is realized by the following technical scheme:

in a first aspect, an embodiment of the present invention provides a chromatographic column device, including a column tube, wherein one end of the column tube is connected to a column neck, the other end of the column tube is connected to a dropping liquid tube, and a bottle mouth connecting column is installed outside the dropping liquid tube; wherein, the pillar pipe is inside to have the back taper cavity, and the back taper cavity sets up the sieve with the link of dropping liquid pipe.

As a further implementation mode, one side of the bottle mouth connecting column is provided with a vent pipe.

As a further implementation mode, one end of the bottle mouth connecting column is connected with the outer wall of the dropping liquid pipe in a sealing mode, and the other end of the bottle mouth connecting column is an outer ground.

As a further realization, the drip tube is provided with a throttle.

As a further implementation, the collar is an inner mill.

As a further implementation, the sieve plate is a sand core sieve plate.

In a second aspect, the embodiment of the present invention further provides a chromatography column apparatus, including the chromatography column device.

As a further implementation manner, the device further comprises a vacuum pump, and the vacuum pump is connected with the vent pipe.

In a third aspect, an embodiment of the present invention further provides a method for using a chromatography column apparatus, in which a filler is filled in an inverted conical cavity inside a column tube, and then a series of experimental operations are performed;

when the flow rate of the elution solution is low, the bottle opening connecting column is tightly connected with the receiving bottle, and the vent pipe is connected with the vacuum pump; and starting the air pump to form pressure difference at two ends of the chromatographic column and increase the flow rate of the chromatographic column.

As a further implementation, the dropping speed is controlled by adjusting the throttle valve in the experimental process.

The beneficial effects of the above-mentioned embodiment of the present invention are as follows:

(1) one or more embodiments of the invention utilize the chromatographic separation principle, increase the number of chromatographic column plates through the tapered structure with thick upper part and thin lower part inside the column tube, improve the chromatographic separation degree;

(2) one or more embodiments of the invention are provided with a bottle mouth connecting column which can be tightly connected with an external ground bottle; and the bottleneck connecting column is connected with a vent pipe, the vent pipe is connected with a vacuum pump, and the flow velocity of the chromatographic column is increased by vacuumizing.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate exemplary embodiments of the invention and together with the description serve to explain the invention and not to limit the invention.

FIG. 1 is a schematic diagram of a chromatography column apparatus according to one or more embodiments of the invention;

wherein, 1-column neck, 2-column tube, 3-sieve plate, 4-dropping tube, 5-throttle valve, 6-vent pipe, 7-bottle mouth connecting column.

Detailed Description

It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application. As used herein, the singular forms "a", "an", and/or "the" are intended to include the plural forms as well, and it should be understood that when the terms "comprises" and/or "comprising" are used in this specification, they specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof;

for convenience of description, the words "up", "down", "left" and "right" in this application, if any, merely indicate correspondence with the directions of up, down, left and right of the drawings themselves, and do not limit the structure, but merely facilitate the description of the invention and simplify the description, and do not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the application.

The terms "mounted", "connected", "fixed", and the like in the present application should be understood broadly, and for example, the terms "mounted", "connected", and "fixed" may be fixedly connected, detachably connected, or integrated; the two components can be connected directly or indirectly through an intermediate medium, or the two components can be connected internally or in an interaction relationship, and the terms can be understood by those skilled in the art according to specific situations.

The first embodiment is as follows:

the embodiment provides a chromatographic column device, as shown in fig. 1, including column neck 1, column pipe 2, dropping liquid pipe 4, bottleneck spliced pole 7, column neck 1, column pipe 2, dropping liquid pipe 4 are connected as an organic whole, and bottleneck spliced pole 7 is fixed in the dropping liquid pipe 4 outside.

Further, the inside back taper cavity that has of pillar pipe 2, back taper cavity one end and 1 intercommunication of column neck, the other end and dropping liquid pipe 4 intercommunication, and the link diameter of back taper cavity and column neck 1 is greater than its link diameter with dropping liquid pipe 4. The inverted cone-shaped cavity is beneficial to chromatographic separation and filling and pouring of the filler.

The sieve plate 3 is arranged at the connecting end of the inverted cone-shaped cavity and the dropping liquid pipe 4 to prevent the filler from leaking. Preferably, the screen plate 3 is a sand core screen plate. The dropping liquid pipe 4 is provided with a throttle valve 5, and the dropping speed of the chromatographic column can be controlled through the throttle valve 5.

The column neck 1 is an inner grinding opening and can be hermetically connected with other external devices. The bottle mouth connecting column 7 comprises a connecting part and a matching part which are connected into a whole, wherein the connecting part is in a frustum shape and is hermetically connected with the outer wall of the dropping liquid pipe 4; a cavity is arranged between the connecting part and the outer wall of the dropping liquid pipe 4. The matching part is in an inverted frustum shape, is an outer ground opening and can be tightly connected with an outer ground opening bottle.

A vent pipe 6 is arranged on one side of the connecting part of the bottle opening connecting column 7, the vent pipe 6 can be connected with a vacuum pump, and the flow rate of the chromatographic column is increased through vacuumizing.

Example two:

the embodiment provides a chromatographic column device, which comprises the chromatographic column device of the embodiment I and further comprises a vacuum pump, wherein the vacuum pump is connected with the vent pipe 6.

Example three:

the present embodiment provides a method for using a chromatography column apparatus, which uses the chromatography column apparatus of the first embodiment, and includes:

when the column is filled, the filler is filled in the column tube 2, and then the experimental processes of activation, sample loading, leaching and the like are carried out.

When the flow rate of the elution solution is slow, the bottle mouth connecting column 7 is tightly connected with the receiving bottle, and the vent pipe 6 is connected with the vacuum pump. And starting the air pump to form pressure difference at two ends of the chromatographic column and increase the flow rate of the chromatographic column.

During the experiment, the dropping speed can be controlled by adjusting the throttle valve 5.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A chromatographic column device is characterized by comprising a column tube, wherein one end of the column tube is connected with a column neck, the other end of the column tube is connected with a dropping liquid tube, and a bottle mouth connecting column is arranged on the outer side of the dropping liquid tube; wherein, the pillar pipe is inside to have the back taper cavity, and the back taper cavity sets up the sieve with the link of dropping liquid pipe.

2. A chromatography column assembly according to claim 1, wherein a vent tube is provided on one side of the spout attachment post.

3. A chromatographic column device according to claim 1 or 2, wherein one end of the bottle mouth connecting column is connected with the outer wall of the dropping liquid pipe in a sealing way, and the other end is an outer grinding port.

4. A chromatography column apparatus according to claim 1, wherein the drip tube is provided with a throttle valve.

5. A chromatography column apparatus according to claim 1, wherein said neck is internally ground.

6. A chromatography column arrangement according to claim 1, wherein said sieve plate is a sand core sieve plate.

7. A chromatography column apparatus comprising a chromatography column device according to any one of claims 1 to 6.

8. The chromatography column apparatus of claim 7, further comprising a vacuum pump connected to the vent tube.

9. A method of using a chromatography column apparatus according to any one of claims 1 to 6, wherein a packing is packed in an inverted conical cavity inside the column tube, followed by a series of experimental operations;

10. The use of a chromatography column apparatus according to claim 9, wherein the dripping speed is controlled by adjusting the throttle valve during the experiment.