CN111887251B - Mixed hormone beneficial to growth of Korean pine - Google Patents

Mixed hormone beneficial to growth of Korean pine Download PDF

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CN111887251B
CN111887251B CN202010872246.3A CN202010872246A CN111887251B CN 111887251 B CN111887251 B CN 111887251B CN 202010872246 A CN202010872246 A CN 202010872246A CN 111887251 B CN111887251 B CN 111887251B
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hormone
growth
gibberellin
indole
benzylaminopurine
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CN111887251A (en
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贾庆彬
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Jilin Provincial Academy of Forestry Sciences
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Jilin Provincial Academy of Forestry Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/06Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
    • A01N43/12Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/10Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/36Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
    • A01N43/38Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N45/00Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to the technical field of hormones, in particular to a mixed hormone beneficial to the growth of Korean pine. The compound comprises 1-3 parts of indole-3-acetic acid, 0.1-1 part of naphthylacetic acid, 1-2 parts of 6-benzylaminopurine and gibberellin-4, 71-2 parts of indole-3-acetic acid, wherein the content range of the naphthylacetic acid is 50-150mg/L, the content range of the naphthylacetic acid is 5-50mg/L, the content range of the 6-benzylaminopurine is 50-100mg/L, the content range of the gibberellin-4, 7 is 50-100mg/L, and the optimal proportion of the indole-3-acetic acid, the naphthylacetic acid, the 6-benzylaminopurine and the gibberellin-4, 7 is 3: 1:2: 2, the invention can promote the growth of the Korean pine aiming at the Korean pine.

Description

Mixed hormone beneficial to growth of Korean pine
Technical Field
The invention relates to the technical field of hormones, in particular to a mixed hormone beneficial to the growth of Korean pine.
Background
Plant hormones, also called plant natural hormones or plant endogenous hormones, refer to some trace organic compounds which are produced in plants and can regulate (promote and inhibit) own physiological processes, and the plant hormones in the prior art are all aimed at plants at present and do not aim at pinus koraiensis alone.
Disclosure of Invention
The present invention is directed to a hormone mixture for promoting the growth of Korean pine, which solves the above problems of the prior art.
In order to achieve the aim, the invention provides a mixed hormone beneficial to the growth of Korean pine, which comprises the following raw materials: 1-3 parts of indole-3-acetic acid, 0.1-1 part of naphthylacetic acid, 1-2 parts of 6-benzylaminopurine and 4, 71-2 parts of gibberellin.
As a further improvement of the technical scheme, the content range of the indole-3-acetic acid is 50-150mg/L, the indole-3-acetic acid is prepared by adding 90g (1.37mol) of 85% potassium hydroxide and 117g (1mol) of indole into a 1L autoclave, adding 120g (1.1mol) of 70% glycolic acid aqueous solution, sealing the autoclave, replacing air with nitrogen, reacting at 250 ℃ for 22h, then cooling to 50 ℃, opening a cover of the autoclave, adding 333mL of water, heating to 100 ℃ for 30min to dissolve crude potassium indole acetate, then cooling to room temperature, pouring out the reaction liquid, washing the autoclave with water, adding water to adjust the volume to 1000mL, then extracting indole with 200mL of diethyl ether, recovering for standby use, acidifying the aqueous potassium salt with concentrated hydrochloric acid, evaporating part of water, cooling to 10 ℃ for crystallization, filtering, collecting the obtained crystals, washing with cold water, and drying to obtain a light yellow crude product of indoleacetic acid with a melting point of about 163-165 ℃. Dissolving with hot water, decolorizing with activated carbon, recrystallizing to obtain almost colorless needle-like indole-3-acetic acid, wherein the indole-3-acetic acid has effects in cell division, cell elongation and differentiation, and elongation, maturation and aging of vegetative organ and reproductive organ, and can be used in tissue culture for inducing callus and root formation.
As a further improvement of the technical scheme, the content range of the naphthylacetic acid is 5-50mg/L, the naphthylacetic acid reacts with chloroacetic acid under the action of heating and a catalyst to generate the naphthylacetic acid, and the naphthylacetic acid is used for promoting the rooting of seeds, the rooting of cuttings and the growing of fibrous roots of solanaceae crops and can promote the rapid expansion of fruits and tuber tubers, so that the naphthylacetic acid can be used as an expanding agent on vegetables and fruit trees. The plant growth regulator can improve the flowering and fruit setting rate, prevent flower and fruit dropping, has the function of preventing fruit dropping, can improve the yield and the quality, promote flourishing branches and leaves and strong plants, and effectively improve the drought resistance, cold resistance, waterlogging resistance, disease resistance, salt and alkali resistance, stress resistance and other capabilities of crops.
As a further improvement of the technical scheme, the content range of the 6-benzylaminopurine is 50-100mg/L, the 6-benzylaminopurine is prepared by adding adenine, alkali and benzyl alcohol in a certain proportion into a three-necked flask, stirring and heating to continuously evaporate water-alcohol azeotrope until the reaction liquid is in a homogeneous transparent state, evaporating excessive benzyl alcohol, adding a large amount of water to dissolve the residue after the residue is cooled, filtering, adjusting the pH of the filtrate to 6 by hydrochloric acid, performing suction filtration on the precipitated precipitate, washing the filter cake with water, then washing with ethanol, and drying to obtain the 6-benzylaminopurine, wherein the 6-benzylaminopurine promotes cell division and induces plant tissue differentiation, is commonly used for plant tissue culture, and is used for improving the result rate in production, promoting fruit growth and keeping vegetables fresh; the action is similar to that of kinetin, but the activity is higher than that of kinetin.
As a further improvement of the technical scheme, the content range of the gibberellin-4, 7 is 50-100mg/L, the gibberellin-4, 7 is fermented in culture media such as bran, cane sugar and inorganic salt by utilizing gibberellin, gibberellin is metabolized to generate gibberellin, fermentation liquor is subjected to solvent extraction and concentration to obtain the gibberellin-4, 7, the gibberellin-4, 7 promotes cell division, cell elongation and leaf expansion, stem extension and side branch growth, bolting and male flower formation, dormancy breaking, seed germination promoting, parthenocarpy and fruit growth promoting, plant maturing rate improving, gibberellin can also inhibit fruit maturation and side bud dormancy, senescence inhibiting, plant block formation inhibiting and rooting inhibiting.
As a further improvement of the technical scheme, the optimal mixture ratio of the indole-3-acetic acid, the naphthylacetic acid, the 6-benzylaminopurine and the gibberellin-4, 7 is 3: 1:2: 2.
compared with the prior art, the invention has the beneficial effects that: the mixed hormone beneficial to the growth of the Korean pine can be added to the Korean pine to promote the growth and development of the Korean pine, has a more positive promoting effect on the high growth of young forests of the Korean pine, is beneficial to the rapid growth of the Korean pine and is convenient for subsequent use.
Drawings
FIG. 1 is a bar graph showing the high growth of young red pine trees after the mixed phytohormone treatment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like, indicate orientations and positional relationships based on those shown in the drawings, and are used only for convenience of description and simplicity of description, and do not indicate or imply that the equipment or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be considered as limiting the present invention.
Referring to fig. 1, the present embodiment provides a hormone mixture for promoting the growth of Korean pine, which comprises the following raw materials: 1-3 parts of indole-3-acetic acid, 0.1-1 part of naphthylacetic acid, 1-2 parts of 6-benzylaminopurine and 4, 71-2 parts of gibberellin.
Further, the content range of the indole-3-acetic acid is 50-150mg/L, the indole-3-acetic acid is prepared by adding 90g (1.37mol) of 85% potassium hydroxide and 117g (1mol) of indole into a 1L autoclave, adding 120g (1.1mol) of 70% glycolic acid aqueous solution, sealing the autoclave, replacing air with nitrogen, reacting at 250 ℃ for 22h, then cooling to 50 ℃, opening a cover of the autoclave, adding 333mL of water, heating to 100 ℃ for 30min to dissolve crude indole acetic acid potassium salt, then cooling to room temperature, pouring out the reaction solution, washing the autoclave with water, adding water to adjust the volume to 1000mL, then extracting indole with 200mL of diethyl ether, recovering for standby use, acidifying aqueous phase potassium salt with concentrated hydrochloric acid, evaporating part of water, cooling to 10 ℃ for crystallization, filtering, collecting the obtained crystals, washing with cold water, drying to obtain a light yellow crude indole acetic acid, the melting point is about 163-165 ℃. Dissolving with hot water, decolorizing with activated carbon, recrystallizing to obtain almost colorless needle-like indole-3-acetic acid, wherein the indole-3-acetic acid has effects in cell division, cell elongation and differentiation, and elongation, maturation and aging of vegetative organ and reproductive organ, and can be used in tissue culture for inducing callus and root formation.
Specifically, the content range of the naphthylacetic acid is 5-50mg/L, naphthalene and chloroacetic acid react under the action of heating and a catalyst to generate the naphthylacetic acid, and the naphthylacetic acid is used for promoting the rooting of seeds, the rooting of cuttings and the fibrous root of solanaceae crops and can promote the rapid expansion of fruits and tuber tubers, so that the naphthylacetic acid can be used as an expanding agent on vegetables and fruit trees. The plant growth regulator can improve the flowering and fruit setting rate, prevent flower and fruit dropping, has the function of preventing fruit dropping, can improve the yield and the quality, promote flourishing branches and leaves and strong plants, and effectively improve the drought resistance, cold resistance, waterlogging resistance, disease resistance, salt and alkali resistance, stress resistance and other capabilities of crops.
Furthermore, the content range of the 6-benzylaminopurine is 50-100mg/L, the 6-benzylaminopurine is obtained by adding adenine, alkali and benzyl alcohol in a certain proportion into a three-necked bottle, stirring and heating to continuously evaporate water-alcohol azeotrope until the reaction liquid is in a homogeneous transparent state, evaporating excessive benzyl alcohol, cooling the residue, adding a large amount of water to dissolve the residue, filtering, adjusting the pH of the filtrate to 6 by hydrochloric acid, performing suction filtration on the precipitated precipitate, washing the filter cake with water, then washing with ethanol, and drying to obtain the 6-benzylaminopurine, wherein the 6-benzylaminopurine promotes cell division and induces plant tissue differentiation, is commonly used for plant tissue culture, and is used for improving the result rate in production and promoting fruit growth and vegetable fresh keeping; the action is similar to that of kinetin, but the activity is higher than that of kinetin.
In addition, the content range of the gibberellin-4, 7 is 50-100mg/L, the gibberellin-4, 7 is obtained by fermenting the gibberellin in culture media such as bran, cane sugar and inorganic salt, the gibberellin is metabolized to generate the gibberellin, fermentation liquor is extracted and concentrated by a solvent to obtain the gibberellin-4, 7, the gibberellin-4, 7 promotes cell division, cell elongation and leaf expansion, stem extension and lateral branch growth, bolting, male flower formation, dormancy breaking, seed germination promoting, parthenocarpy and fruit growth promoting and plant maturing rate improving, and the gibberellin can also inhibit fruit maturation and lateral bud dormancy, aging, plant block formation and rooting.
It is worth to be noted that the optimal mixture ratio of the indole-3-acetic acid, the naphthylacetic acid, the 6-benzylaminopurine and the gibberellin-4, 7 is 3: 1:2: 2.
the experimental cases of different combinations and concentrations of phytohormones on the growth of young forests of Korean pine are as follows:
carrying out a plant hormone regulation and control comparison test on young forests of 10-year-old red pine, carrying out tree height and ground diameter investigation on test forest stands, using the plant hormone as early basic data of the hormone treatment comparison test, carrying out a hormone treatment test on the test forests for 1 time every 10 days from the growth start of 5-day red pine at 5 months to the basic growth stop at 25-7 months, wherein the plant hormones select indole-3-acetic acid, naphthylacetic acid, 6-benzylaminopurine and gibberellin-4, 7, each hormone is provided with 3 concentration gradients (50mg/L, 100mg/L and 150mg/L), the selected hormones are combined according to orthogonal design by mixed application (detailed in table 1), 9 combinations are totally, 1 blank control is designed, 10 treatments are totally designed, and the mixed hormone treatment test result shows that: the difference exists between different treatments, and the high growth amount of the treated young red pine forest in the year is obviously improved compared with the control (Table 2).
TABLE 1 Mixed hormone treatment orthogonal design Table
Figure BDA0002651492150000051
Wherein: a: indole-3-acetic acid, b: naphthylacetic acid, c: gibberellin-4, 7, d: 6-benzylaminopurine; 1: 50mg/L, 2: 100mg/L, 3: 150mg/L
TABLE 2 high annual growth of trees after phytohormone treatment in different combinations
Figure BDA0002651492150000052
Figure BDA0002651492150000061
The results of the anova show (table 3): the high growth amounts of the Korean pine are obviously different among different treatments (P is less than 0.05), and multiple comparisons are carried out on various hormone combinations by a Duncan method (Table 4), and the results show that the high growth amounts of the Korean pine are all improved after the phytohormone treatments, wherein the improvement effect of the B combination (150mg/L indole-3-acetic acid, 50mg/L naphthylacetic acid, 100mg/L gibberellin-4, 7, and 100 mg/L6-benzylaminopurine) is the best, the average high growth amount is 40.03cm, is higher than that of CK1 by 36.67 percent, the F combination is 24.98 percent, is higher than that of ginseng test plants by 15.66 percent, and has an obvious effect of promoting the high growth of young forests of the Korean pine (figure 1).
TABLE 3 analysis of variance of high annual growth of trees after treatment with different hormone combinations
Figure BDA0002651492150000062
TABLE 4 multiple comparison of high annual growth of trees after treatment with different combination hormones
Figure BDA0002651492150000063
Figure BDA0002651492150000071
As shown in figure 1, the phytohormone combination of 150mg/L IAA, 50mg/L NAA, 100mg/L GA (4+7) and 100 mg/L6-BA has positive promotion effect on the high growth of the young red pine forest after the mixed phytohormone treatment.
The test cases of the growth amount of young red pine forest by using single phytohormone with different concentrations are as follows:
the treatment effect of single hormone is tested while the mixed hormone treatment is carried out, the test method is the same as the mixed hormone treatment process, the plant hormones adopt indole-3-acetic acid, naphthylacetic acid, 6-benzylaminopurine and gibberellin-4, 7, each hormone is provided with 3 concentration gradients (50mg/L, 100mg/L and 150mg/L) for 12 treatments, and blank controls are provided for 1 treatment (CK 2).
TABLE 5 high annual growth of trees treated with different concentrations of a single phytohormone
Figure BDA0002651492150000072
Figure BDA0002651492150000081
Wherein, IAA is indole-3-acetic acid, NAA is naphthylacetic acid, 6-BA is 6-benzylaminopurine, GA (4+7) is gibberellin-4, 7.
The analysis of variance results show (table 6): significant differences in high growth of pinus koraiensis were observed between treatments with different concentrations of single hormone (P <0.05), and multiple comparisons of each hormone combination were performed using the Duncan method (table 7), showing: after single phytohormone treatment with different concentrations, the high growth amount of the Korean pine is improved compared with a control, wherein the improvement effect of 100mg/L IAA, 100 mg/L6-BA and 150mg/L IAA is better, the difference is obvious from the control CK2, and the difference between other treatments and the control does not reach the obvious level. The high average growth amount of trees is 32.31cm after 100mg/L IAA treatment with better performance, although single hormone treatment has a promoting effect on the high growth of the Korean pine, the effect is obviously lower than that of the trees after mixed hormone treatment, the high average growth amount of trees after 150mg/L IAA, 50mg/L NAA, 100mg/L GA (4+7) and 100 mg/L6-BA mixed hormone treatment is 40.03cm, and the high average growth amount of trees is 23.89% higher than that of the trees after 150mg/L IAA treatment with the best performance in single hormone treatment.
TABLE 6 analysis of variance of tree growth in high years after single hormone treatment at different concentrations
Figure BDA0002651492150000091
TABLE 7 multiple comparison of high annual growth of trees after single hormone treatment at different concentrations
Figure BDA0002651492150000092
Figure BDA0002651492150000101
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and the preferred embodiments of the present invention are described in the above embodiments and the description, and are not intended to limit the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (6)

1. A mixed hormone beneficial to the growth of Korean pine comprises the following raw materials: 1-3 parts of indole-3-acetic acid, 0.1-1 part of naphthylacetic acid, 1-2 parts of 6-benzylaminopurine and 4, 71-2 parts of gibberellin.
2. The hormone cocktail of claim 1 wherein said hormone is selected from the group consisting of: the content range of the indole-3-acetic acid is 50-150 mg/L.
3. The hormone cocktail of claim 1 wherein said hormone is selected from the group consisting of: the content range of the naphthylacetic acid is 5-50 mg/L.
4. The hormone cocktail of claim 1 wherein said hormone is selected from the group consisting of: the content range of the 6-benzylaminopurine is 50-100 mg/L.
5. The hormone cocktail of claim 1 wherein said hormone is selected from the group consisting of: the content range of the gibberellin-4, 7 is 50-100 mg/L.
6. The hormone cocktail of claim 1 wherein said hormone is selected from the group consisting of: the mixture ratio of the indole-3-acetic acid, the naphthylacetic acid, the 6-benzylaminopurine and the gibberellin-4, 7 is 3: 1:2: 2.
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