CN115119844B - Seedling antifreeze fluid and preparation method thereof - Google Patents
Seedling antifreeze fluid and preparation method thereof Download PDFInfo
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- CN115119844B CN115119844B CN202210861562.XA CN202210861562A CN115119844B CN 115119844 B CN115119844 B CN 115119844B CN 202210861562 A CN202210861562 A CN 202210861562A CN 115119844 B CN115119844 B CN 115119844B
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- hydroxyproline
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
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- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a nursery stock antifreeze, which comprises L-hydroxyproline and calcium salt; the content of L-hydroxyproline is 40-80mg/L. In addition, the preparation method of the seedling antifreeze fluid is also disclosed, comprising the following steps: mixing L-hydroxyproline, spermidine, jasmonic acid, calcium chloride and rhamnolipid uniformly. Compared with the prior art, the seedling antifreeze fluid has lower freezing injury index and freezing injury rate.
Description
Technical Field
The invention belongs to the technical field of chemical antifreezing; relates to a nursery stock antifreezing solution and a preparation method thereof.
Background
Temperature is one of the important factors affecting plant growth and distribution. The proper temperature can promote the growth of plants, and the plants grow slowly when the temperature is reduced; below the tolerance temperature of the plant, the plant may become damaged or even die, a condition known as low temperature stress. Plants are subjected to varying degrees of low temperature stress each year, causing agricultural losses in the thousands of billions of dollars each year to the world. Low temperature stress includes freeze injury and cold injury. The damage to plant cells and tissues when the temperature is lower than 0 ℃ is called freezing injury; cold damage refers to damage to plants caused by temperatures above 0 ℃.
The winter in the alpine region of the Qinghai-Tibet plateau in China is cold, and the seedlings are more prone to being frozen; in spring, the seedlings are more easily suffered from cold injury. In order to prevent the seedlings from frost and physiological drought in winter and spring, cold-proof measures are taken for the seedlings of some conifer species and broadleaf species with weak cold resistance.
In recent years, with continuous progress of the technology level, winter cold-proof technology of plants is gradually improved, and the effects of cold prevention and freezing resistance are also better. The nursery stock winter protection can be realized by burying soil, covering grass, setting up a windbreak, setting up a greenhouse, fumigating and irrigating, and the like. These methods belong to the physical freeze protection methods, newer methods include the use of chemical or biological cold protection freeze protection methods.
Chinese patent application publication No. CN101199284A discloses an antifreezing agent for flowers and fruits of apricot trees for kernels, which is prepared by mixing abscisic acid, brassinolide, cerium nitrate and water, wherein the concentration of the abscisic acid is 10mg/L, the concentration of the brassinolide is 1.6mg/L, and the concentration of the cerium nitrate is 0.8mg/L. The invention has reasonable medicine compatibility and high medicine utilization rate, can obviously reduce the frozen flower rate of the kernel apricot and can obviously improve the fruit setting rate of the kernel apricot. The plant frozen flower rate of the agent is reduced by 38-66% compared with clear water, the fruit setting rate is improved by 103.8% compared with clear water, and the used raw materials have strong permeation, internal absorption, high efficiency, broad spectrum, low toxicity and safety, low manufacturing cost and convenient use. The apricot tree is suitable for apricot trees for various kernels, and other fruit trees can be used by reference.
Chinese patent application publication CN108934544a discloses a method for preventing seedlings from being cold and freezing, which comprises spraying a cold-resistant antifreezing agent a and a cold-resistant antifreezing agent B at different times respectively; the cold-resistant antifreezing agent A is used for inhibiting the seedlings Gao Shengchang in the middle and later stages of the growing season of the seedlings, so that internodes are shortened; promoting differentiation and division of cells at rhizome parts, promoting rooting of wood, thickening of stems and formation of cell walls, and improving the lignification degree of seedlings; the cold-resistant antifreezing agent B is used for promoting leaf shedding, bud dormancy and stomata closure in autumn and accelerating physiological effects of maturation, shedding and aging, thereby accelerating seedling lignification and preventing and treating seedling epidemic diseases. The invention is applied at different stages of growing season respectively, and adjusts the growth and physiological activities of the nursery stock. In the middle and later stages of the growing season, the overgrowth of the seedlings is controlled, the diameter-height ratio is increased, the growth of lateral roots is promoted, the leaf falling of the seedlings and the dormancy of leaf buds are timely promoted in autumn, the maturity of the seedlings is accelerated, the lignification degree is improved, and the stress resistance of the seedlings such as cold resistance and freezing resistance is remarkably improved.
However, the chemical antifreezing agent in the prior art still has the technical defect of poor cold-proof and freezing-proof effects.
Disclosure of Invention
The invention aims to provide a nursery stock antifreezing solution and a preparation method thereof. Compared with the prior art, the seedling antifreeze fluid has lower freezing injury index and freezing injury rate.
In order to achieve the above object, on the one hand, the technical scheme adopted by the invention is as follows: the nursery stock antifreezing solution comprises L-hydroxyproline and calcium salt.
The nursery stock antifreeze fluid disclosed by the invention has the L-hydroxyproline content of 40-80mg/L.
Preferably, the L-hydroxyproline content is 50-60mg/L.
The seedling antifreeze fluid disclosed by the invention has the calcium salt content of 700-900mg/L.
Preferably, the calcium salt is present in an amount of 750-850mg/L.
The nursery stock antifreeze fluid disclosed by the invention, wherein the formula further comprises arginin.
The seedling antifreeze fluid disclosed by the invention has the content of the spermidine of 20-40mg/L.
Preferably, the content of the spermidine is 25-35mg/L.
The nursery stock antifreeze fluid disclosed by the invention, wherein the formula further comprises jasmonic acid.
The nursery stock antifreezing solution disclosed by the invention has the advantage that the jasmonic acid content is 10-30mg/L.
Preferably, the jasmonic acid content is 15-25mg/L.
The nursery stock antifreeze fluid disclosed by the invention, wherein the formula further comprises rhamnolipid.
The nursery stock antifreeze fluid disclosed by the invention has the content of the rhamnolipid of 80-110mg/L.
Preferably, the content of the rhamnolipid is 90-100mg/L.
The invention relates to a seedling antifreeze fluid, which comprises the following components in percentage by weight:
50-60mg/L of L-hydroxyproline;
25-35mg/L of spermidine;
15-25mg/L of jasmonic acid;
750-850mg/L of calcium chloride;
90-100mg/L of rhamnolipid;
the balance being water.
In another aspect, the invention provides a preparation method of the seedling antifreeze, which comprises the following steps:
adding L-hydroxyproline, arginine, jasmonic acid, calcium chloride and rhamnolipid in the formula amount into a proper amount of water, stirring to dissolve and mix uniformly, adding the rest of water, and stirring to mix uniformly.
In still another aspect, the invention provides an application of the seedling antifreeze fluid, wherein the antifreeze fluid is used for antifreeze of eucommia seedlings.
Compared with the prior art, the seedling antifreeze fluid has lower freezing injury index and freezing injury rate.
Detailed Description
It must be noted that, as used in this specification and the appended claims, the singular forms "a," "an," and "the" include both a reference and a plurality of references (i.e., more than two, including two) unless the context clearly dictates otherwise.
Unless otherwise indicated, the numerical ranges in the present invention are approximate, and thus values outside the ranges may be included. The numerical ranges may be expressed herein as from "about" one particular value, and/or to "about" another particular value. When such a range is expressed, another aspect includes from the one particular value and/or to the other particular value. Similarly, when values are expressed as approximations, by use of the antecedent "about," it will be understood that the particular value forms another aspect. It will also be understood that the endpoints of each of the numerical ranges are significant both in relation to the other endpoint, and independently of the other endpoint.
References in the specification and the claims to parts by weight of a particular element or component in a composition or article refer to the relationship by weight between that element or component and any other element or component in the composition or article.
In the present invention, unless specifically indicated to the contrary, or implied by the context of the context or conventional means in the art, the solutions referred to in the present invention are aqueous solutions; when the solute of the aqueous solution is a liquid, all fractions and percentages are by volume, and the volume percent of the component is based on the total volume of the composition or product comprising the component; when the solute of the aqueous solution is a solid, all fractions and percentages are by weight, and the weight percentages of the components are based on the total weight of the composition or product comprising the components.
References to "comprising," "including," "having," and similar terms in this invention are not intended to exclude the presence of any optional components, steps or procedures, whether or not any optional components, steps or procedures are specifically disclosed. For the avoidance of any doubt, unless stated to the contrary, all methods claimed through use of the term "comprising" may include one or more additional steps, apparatus parts or components and/or materials. In contrast, the term "consisting of … …" excludes any component, step or procedure not specifically recited or enumerated. The term "or" refers to members recited individually as well as in any combination unless otherwise specified.
Furthermore, the contents of any of the referenced patent documents or non-patent documents in the present invention are incorporated by reference in their entirety, especially with respect to the definitions and general knowledge disclosed in the art (in case of not inconsistent with any definitions specifically provided by the present invention).
In the present invention, parts are parts by weight unless otherwise indicated, temperatures are expressed in degrees celsius or at ambient temperature, and pressures are at or near atmospheric. Room temperature represents 20-30 ℃. There are numerous variations and combinations of reaction conditions (e.g., component concentrations, solvents needed, solvent mixtures, temperatures, pressures, and other reaction ranges) and conditions that can be used to optimize the purity and yield of the product obtained by the process. Only reasonable routine experimentation will be required to optimize such process conditions.
In the present invention, the biosurfactant bisrhamnolipid (Rha-C10) was purchased from tsucan biotechnology limited under the name R650, purity 98%.
Example 1
The formula of the seedling antifreeze fluid is as follows:
l-hydroxyproline 60mg/L;
25mg/L of spermidine;
15mg/L of jasmonic acid;
800mg/L of calcium chloride;
100mg/L of rhamnolipid;
the balance being water.
The preparation method of the seedling antifreeze fluid comprises the following steps:
adding L-hydroxyproline, arginine, jasmonic acid, calcium chloride and rhamnolipid in the formula amount into a proper amount of water, stirring to dissolve and mix uniformly, adding the rest of water, and stirring to mix uniformly.
Example 2
The formula of the seedling antifreeze fluid is as follows:
l-hydroxyproline 50mg/L;
35mg/L of spermidine;
jasmonic acid 25mg/L;
800mg/L of calcium chloride;
90mg/L of rhamnolipid;
the balance being water.
The preparation method of the seedling antifreeze fluid comprises the following steps:
adding L-hydroxyproline, arginine, jasmonic acid, calcium chloride and rhamnolipid in the formula amount into a proper amount of water, stirring to dissolve and mix uniformly, adding the rest of water, and stirring to mix uniformly.
Comparative example 1
The formula of the seedling antifreeze fluid is as follows:
85mg/L of L-hydroxyproline;
15mg/L of jasmonic acid;
800mg/L of calcium chloride;
100mg/L of rhamnolipid;
the balance being water.
The preparation method of the seedling antifreeze fluid comprises the following steps:
adding L-hydroxyproline, jasmonic acid, calcium chloride and rhamnolipid in the formula amount into a proper amount of water, stirring to dissolve and mix uniformly, adding the rest of water, and stirring to mix uniformly.
Comparative example 2
The formula of the seedling antifreeze fluid is as follows:
l-hydroxyproline 75mg/L;
25mg/L of spermidine;
800mg/L of calcium chloride;
100mg/L of rhamnolipid;
the balance being water.
The preparation method of the seedling antifreeze fluid comprises the following steps:
adding L-hydroxyproline, arginine, calcium chloride and rhamnolipid in the formula amount into a proper amount of water, stirring to dissolve and uniformly mix the components, adding the rest of water, and stirring to uniformly mix the components.
Comparative example 3
The formula of the seedling antifreeze fluid is as follows:
l-hydroxyproline 60mg/L;
25mg/L of spermidine;
15mg/L of jasmonic acid;
900mg/L of calcium chloride;
the balance being water.
The preparation method of the seedling antifreeze fluid comprises the following steps:
adding L-hydroxyproline, arginine, jasmonic acid and calcium chloride in the formula amount into a proper amount of water, stirring to dissolve and mix uniformly, adding the rest of water, and stirring to mix uniformly.
Effect test
Local 2-year-old eucommia seedlings 1200 were selected and subjected to 6 treatments (examples 1-2, comparative examples 1-3 and clear water control group), 200 each. Spraying in the first 10 months of the year, each treatment was performed 3 times at intervals of 10 days. And 5 months after the next year, counting the freezing injury condition of eucommia seedlings. The freezing injury of the seedlings is classified as 4 grades. The length of the dry tip of the eucommia ulmoides nursery stock is measured, the dry tip reaches the level 1 of the freezing injury of the whole plant, the level 2 of the freezing injury of the whole plant, the level 3 of the freezing injury of the whole plant, the level 4 of the freezing injury of the whole plant, the freezing injury index and the freezing injury rate are calculated according to the following formula, wherein the height 1/5 of the whole plant is the level 1 of the freezing injury, and the height 1/3 of the whole plant is the level 3 of the freezing injury.
Freeze injury index = Σ (number of freeze injury plants x number of freeze injury stages)/(total number of freeze injury plants x 4);
freezing injury rate = Σ (freezing injury number of freezing injury level not less than 2 level)/investigation number of plants x 100%.
The results are shown in Table 1.
TABLE 1
| Index of freeze injury | Freeze injury rate (%) | |
| Example 1 | 0.19 | 5.5 |
| Example 2 | 0.22 | 6.0 |
| Comparative example 1 | 0.31 | 9.5 |
| Comparative example 2 | 0.35 | 11.5 |
| Comparative example 3 | 0.41 | 15.0 |
| Clear water control group | 0.53 | 21.5 |
As can be seen from Table 1, the nursery stock antifreeze of the embodiment 1-2 of the present invention has lower freeze injury index and freeze injury rate than the comparative examples 1-3 and the fresh water control group.
Further, it should be understood that various changes, substitutions, omissions, modifications, or adaptations to the present invention may be made by those skilled in the art after having read the present disclosure, and such equivalent embodiments are within the scope of the present invention as defined in the appended claims.
Claims (2)
1. The nursery stock antifreezing solution is characterized by comprising the following components in percentage by weight:
50-60mg/L of L-hydroxyproline;
25-35mg/L of spermidine;
15-25mg/L of jasmonic acid;
750-850mg/L of calcium chloride;
90-100mg/L of rhamnolipid;
the balance being water.
2. A method for preparing the seedling anti-freezing solution according to claim 1, which comprises the following steps:
adding L-hydroxyproline, arginine, jasmonic acid, calcium chloride and rhamnolipid in the formula amount into a proper amount of water, stirring to dissolve and mix uniformly, adding the rest of water, and stirring to mix uniformly.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210861562.XA CN115119844B (en) | 2022-07-20 | 2022-07-20 | Seedling antifreeze fluid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202210861562.XA CN115119844B (en) | 2022-07-20 | 2022-07-20 | Seedling antifreeze fluid and preparation method thereof |
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| CN115119844A CN115119844A (en) | 2022-09-30 |
| CN115119844B true CN115119844B (en) | 2023-08-11 |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103027032A (en) * | 2012-09-29 | 2013-04-10 | 浙江大学 | Application of rhamnolipid serving as protective agent of low-temperature or ultralow-temperature cell preservation |
| CN106116829A (en) * | 2016-06-22 | 2016-11-16 | 武汉农尚环境股份有限公司 | A kind of freeze proof reagent of ornamental plant and compound method thereof |
| CN107223666A (en) * | 2017-06-27 | 2017-10-03 | 湖南省蔬菜研究所 | A kind of composition for improving chilli seedling winter resistance and its preparation method and application |
| CN108934544A (en) * | 2018-04-27 | 2018-12-07 | 河北省林业科学研究院 | A kind of nursery stock cold-resistant anti-freezing method |
| CN109924199A (en) * | 2017-12-16 | 2019-06-25 | 新昌县汉良苗木专业合作社 | A kind of nursery stock anti-icing fluid |
| CN111543575A (en) * | 2020-04-10 | 2020-08-18 | 浙江大学舟山海洋研究中心 | Antifreezing solution for freezing food |
-
2022
- 2022-07-20 CN CN202210861562.XA patent/CN115119844B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103027032A (en) * | 2012-09-29 | 2013-04-10 | 浙江大学 | Application of rhamnolipid serving as protective agent of low-temperature or ultralow-temperature cell preservation |
| CN106116829A (en) * | 2016-06-22 | 2016-11-16 | 武汉农尚环境股份有限公司 | A kind of freeze proof reagent of ornamental plant and compound method thereof |
| CN107223666A (en) * | 2017-06-27 | 2017-10-03 | 湖南省蔬菜研究所 | A kind of composition for improving chilli seedling winter resistance and its preparation method and application |
| CN109924199A (en) * | 2017-12-16 | 2019-06-25 | 新昌县汉良苗木专业合作社 | A kind of nursery stock anti-icing fluid |
| CN108934544A (en) * | 2018-04-27 | 2018-12-07 | 河北省林业科学研究院 | A kind of nursery stock cold-resistant anti-freezing method |
| CN111543575A (en) * | 2020-04-10 | 2020-08-18 | 浙江大学舟山海洋研究中心 | Antifreezing solution for freezing food |
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