CN115119844A - Nursery stock antifreezing solution and preparation method thereof - Google Patents
Nursery stock antifreezing solution and preparation method thereof Download PDFInfo
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- CN115119844A CN115119844A CN202210861562.XA CN202210861562A CN115119844A CN 115119844 A CN115119844 A CN 115119844A CN 202210861562 A CN202210861562 A CN 202210861562A CN 115119844 A CN115119844 A CN 115119844A
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- nursery stock
- hydroxyproline
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/04—Nitrogen directly attached to aliphatic or cycloaliphatic carbon atoms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/42—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/06—Aluminium; Calcium; Magnesium; Compounds thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
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- Life Sciences & Earth Sciences (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a nursery stock antifreezing solution, which comprises L-hydroxyproline and calcium salt; the content of L-hydroxyproline is 40-80 mg/L. In addition, the preparation method of the nursery stock antifreezing solution is also disclosed, and comprises the following steps: uniformly mixing the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid according to the formula ratio. Compared with the prior art, the seedling antifreeze fluid has lower freeze injury index and freeze injury rate.
Description
Technical Field
The invention belongs to the technical field of chemical antifreezing; relates to a nursery stock anti-freezing solution and a preparation method thereof.
Background
Temperature is one of the important factors affecting plant growth and distribution. The proper temperature can promote the growth of plants, and the plants grow slowly when the temperature is reduced; when the temperature is lower than the tolerance temperature of the plant, the plant is damaged and even dies, and the condition is called low-temperature stress. Plants are stressed at different levels of low temperature each year, causing agricultural losses of hundreds of billions of dollars each year around the world. Low temperature stress includes freeze injury and cold injury. The damage to plant cells and tissues when the temperature is lower than 0 ℃ is called freezing damage; cold damage refers to damage to plants caused by temperatures above 0 ℃.
In the alpine regions of the Qinghai-Tibet plateau of China, the plants are cold in winter, and the seedlings are easy to suffer from freezing damage; in spring, due to wind-cold, the nursery stock is easy to suffer cold damage. In order to prevent the seedlings from frost and physiological drought in winter and spring, some seedlings of conifer species and broadleaf species with weak cold resistance need cold-proof measures.
In recent years, with the continuous progress of the technology level, the winter protection technology of plants is gradually improved, and the cold protection and freezing resistance effects are better and better. The nursery stock can be protected from cold by burying soil, covering grass, setting windbreak, setting greenhouse, smoking, irrigating and the like. These methods belong to the physical freeze-proofing methods, and newer methods include the use of chemical or biological cold-proofing methods.
Chinese patent application publication CN101199284A discloses a flower and fruit antifreezing agent for kernel apricot trees, which is prepared by mixing abscisic acid, brassinolide, cerium nitrate and water, wherein the concentration of the abscisic acid is 10mg/L, the concentration of the brassinolide is 1.6mg/L, and the concentration of the cerium nitrate is 0.8 mg/L. The invention has the advantages of reasonable medicament compatibility, high medicament utilization rate, capability of obviously reducing the frost flower rate of the kernel apricot and obviously improving the fruit setting rate of the kernel apricot. The plant frost flower rate of the plant using the agent is reduced by 38-66% compared with that of clear water, the fruit setting rate is improved by 103.8% compared with that of clear water, and the plant using the agent has the advantages of strong penetration, internal absorption, high efficiency, broad spectrum, low toxicity, safety, low manufacturing cost and convenient use. Is suitable for various apricot trees for kernel, and other fruit trees can also be used as reference.
Chinese patent application publication CN108934544A discloses a method for resisting cold and preventing freezing of nursery stock, which comprises respectively spraying a cold-resistant antifreezing agent A and a cold-resistant antifreezing agent B at different times; wherein the cold-resistant antifreezing agent A is used for inhibiting high growth of the nursery stock in the middle and later growth seasons of the nursery stock and shortening internodes; promoting the cell differentiation and division of the rhizome part, promoting the wood rooting, the stem thickening and the cell wall formation, and improving the lignification degree of the nursery stock; the cold-resistant antifreezing agent B is used for promoting leaf abscission, bud dormancy and stomatal closure in autumn and accelerating the physiological effects of maturity, abscission and aging, thereby accelerating the lignification of nursery stocks and preventing and treating the epidemic diseases of the nursery stocks. The invention is applied in different stages of growing season to regulate the growth and physiological activity of seedling. In the middle and later growth periods of the growing season, the excessive growth of the nursery stock is controlled, the diameter-height ratio is increased, the growth of lateral roots is promoted, the leaf falling and leaf bud dormancy of the nursery stock are promoted in proper time in autumn, the maturity of the nursery stock is accelerated, the lignification degree is improved, and the stress resistance of the nursery stock such as cold resistance, freezing resistance and the like is obviously improved.
However, the chemical antifreeze of the prior art still has the technical defect of poor cold-proof and freeze-proof effects.
Disclosure of Invention
The invention aims to provide a nursery stock antifreezing solution and a preparation method thereof. Compared with the prior art, the seedling antifreeze fluid has lower freeze injury index and freeze injury rate.
In order to achieve the above object, in one aspect, the technical solution adopted by the present invention is as follows: a nursery stock anti-freezing solution comprises L-hydroxyproline and calcium salt.
The seedling antifreezing solution provided by the invention has the advantage that the content of L-hydroxyproline is 40-80 mg/L.
Preferably, the content of L-hydroxyproline is 50-60 mg/L.
The seedling antifreezing solution provided by the invention has the advantages that the content of calcium salt is 700-900 mg/L.
Preferably, the calcium salt content is 750-850 mg/L.
The seedling antifreezing solution provided by the invention further comprises spermidine.
The seedling antifreezing solution provided by the invention has the advantage that the content of the spermidine is 20-40 mg/L.
Preferably, the content of spermidine is 25-35 mg/L.
The seedling antifreezing solution provided by the invention further comprises jasmonic acid.
The seedling antifreezing solution provided by the invention has the advantage that the content of jasmonic acid is 10-30 mg/L.
Preferably, the jasmonic acid content is 15-25 mg/L.
The nursery stock antifreezing solution provided by the invention further comprises bis rhamnolipid.
The nursery stock antifreezing solution provided by the invention has the advantages that the content of the dirhamnolipid is 80-110 mg/L.
Preferably, the content of the dirhamnolipid is 90-100 mg/L.
The seedling antifreezing solution provided by the invention comprises the following formula:
50-60mg/L of L-hydroxyproline;
25-35mg/L of spermidine;
15-25mg/L jasmonic acid;
750 mg/L of calcium chloride and 850mg/L of calcium chloride;
the dirhamnolipid accounts for 90-100 mg/L;
the balance being water.
On the other hand, the invention provides a preparation method of the nursery stock antifreezing solution, which comprises the following steps:
adding the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid into a proper amount of water according to the formula ratio, stirring to dissolve the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid, uniformly mixing, adding the rest water, and stirring to uniformly mix.
In another aspect, the invention provides an application of the eucommia ulmoides seedling antifreezing solution, wherein the eucommia ulmoides seedling antifreezing solution is used for antifreezing of eucommia ulmoides seedlings.
Compared with the prior art, the seedling antifreeze fluid has lower freeze injury index and freeze injury rate.
Detailed Description
It must be noted that, as used in this specification and the appended claims, the singular forms "a," "an," and "the" include both one and more than one (i.e., two, including two) unless the context clearly dictates otherwise.
Unless otherwise indicated, the numerical ranges in this disclosure are approximate and, thus, may include values outside of the stated ranges. The numerical ranges may be stated herein as from "about" one particular value, and/or to "about" another particular value. When such a range is expressed, another aspect includes from the one particular value and/or to the other particular value. Similarly, when values are expressed as approximations, by use of the antecedent "about," it will be understood that the particular value forms another aspect. It will be further understood that the endpoints of each of the numerical ranges are significant both in relation to the other endpoint, and independently of the other endpoint.
Reference in the specification and concluding claims to parts by weight of a particular element or component in a composition or article refers to the weight relationship between that element or component and any other elements or components in the composition or article, expressed as parts by weight.
In the present invention, unless specifically indicated to the contrary, or implied from the context or customary practice in the art, all solutions referred to herein are aqueous solutions; when the solute of the aqueous solution is a liquid, all fractions and percentages are by volume and the volume percentages of a component are based on the total volume of the composition or product in which it is contained; when the solute of the aqueous solution is a solid, all fractions and percentages are by weight, and the weight percentages of a component are based on the total weight of the composition or product in which the component is included.
References to "comprising," "including," "having," and similar terms in this specification are not intended to exclude the presence of any optional components, steps or procedures, whether or not any optional components, steps or procedures are specifically disclosed. In order to avoid any doubt, all methods claimed through use of the term "comprising" may include one or more additional steps, apparatus parts or components and/or materials unless stated to the contrary. In contrast, the term "consisting of … …" excludes any component, step, or procedure not specifically recited or recited. Unless otherwise specified, the term "or" refers to the listed members individually as well as in any combination.
Furthermore, the contents of any referenced patent or non-patent document in this application are incorporated by reference in their entirety, especially with respect to definitions disclosed in the art (where not inconsistent with any definitions specifically provided herein) and general knowledge.
In the present invention, parts are parts by weight unless otherwise indicated, temperatures are indicated in ° c or at ambient temperature, and pressures are at or near atmospheric. The room temperature means 20-30 ℃. There are many variations and combinations of reaction conditions (e.g., component concentrations, desired solvents, solvent mixtures, temperatures, pressures, and other reaction ranges) and conditions that can be used to optimize the purity and yield of the product obtained by the process. Only reasonable routine experimentation will be required to optimise such process conditions.
In the invention, the biosurfactant dirhamnolipid (Rha-Rha-C10-C10) is purchased from Sichuan Sanson Biotech Co., Ltd, is named as R650, and has the purity of 98%.
Example 1
The formula of the seedling antifreezing solution is as follows:
l-hydroxyproline 60 mg/L;
25mg/L of spermidine;
jasmonic acid 15 mg/L;
800mg/L of calcium chloride;
100mg/L of dirhamnolipid;
the balance being water.
The preparation method of the seedling antifreezing solution comprises the following steps:
adding the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid into a proper amount of water according to the formula ratio, stirring to dissolve the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid, uniformly mixing, adding the rest water, and stirring to uniformly mix.
Example 2
The formula of the seedling antifreezing solution is as follows:
l-hydroxyproline 50 mg/L;
35mg/L of spermidine;
25mg/L jasmonic acid;
800mg/L of calcium chloride;
90mg/L of dirhamnolipid;
the balance being water.
The preparation method of the seedling antifreezing solution comprises the following steps:
adding the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid into a proper amount of water according to the formula ratio, stirring to dissolve the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid, uniformly mixing, adding the rest water, and stirring to uniformly mix.
Comparative example 1
The formula of the seedling antifreezing solution is as follows:
l-hydroxyproline 85 mg/L;
jasmonic acid 15 mg/L;
800mg/L of calcium chloride;
100mg/L of dirhamnolipid;
the balance being water.
The preparation method of the seedling antifreezing solution comprises the following steps:
adding the L-hydroxyproline, the jasmonic acid, the calcium chloride and the dirhamnolipid into a proper amount of water according to the formula ratio, stirring to dissolve and uniformly mix, adding the rest water, and stirring to uniformly mix.
Comparative example 2
The formula of the seedling antifreeze fluid is as follows:
l-hydroxyproline 75 mg/L;
25mg/L of spermidine;
800mg/L of calcium chloride;
100mg/L of dirhamnolipid;
the balance being water.
The preparation method of the seedling antifreezing solution comprises the following steps:
adding the L-hydroxyproline, the spermidine, the calcium chloride and the dirhamnolipid into a proper amount of water according to the formula, stirring to dissolve and uniformly mix, adding the rest water, and stirring to uniformly mix.
Comparative example 3
The formula of the seedling antifreeze fluid is as follows:
l-hydroxyproline 60 mg/L;
25mg/L of spermidine;
jasmonic acid 15 mg/L;
900mg/L of calcium chloride;
the balance being water.
The preparation method of the seedling antifreezing solution comprises the following steps:
adding the L-hydroxyproline, the spermidine, the jasmonic acid and the calcium chloride into a proper amount of water according to the formula ratio, stirring to dissolve and uniformly mix, adding the rest water, and stirring to uniformly mix.
Effect test
1200 local 2-year old eucommia seedlings were selected and treated 6 times (examples 1-2, comparative examples 1-3 and clear water control group), 200 plants each. The spray is applied in the first 10 months of the year, each treatment is carried out for 3 times, and each time interval is 10 days. And 5, counting the freezing damage condition of the eucommia seedlings in the next 5 months. The freezing damage condition of the nursery stock is classified into 4 grades. Measuring the dry shoot length of the eucommia ulmoides seedling, wherein the dry shoot reaches the whole plant height of 1/5, is freezing injury grade 1, the dry shoot reaches the whole plant height of 1/3, is freezing injury grade 2, the dry shoot reaches the whole plant height of 1/2, is freezing injury grade 3, and the whole plant dies is freezing injury grade 4, and calculating the freezing injury index and the freezing injury rate according to the following formula.
The freezing injury index is ∑ (number of freezing injury plants × number of freezing injury stages)/(total number of freezing injury plants × 4);
the freezing injury rate is ∑ (freezing injury number of plants with freezing injury number more than or equal to 2) per investigation number multiplied by 100%.
The results are shown in Table 1.
TABLE 1
| Index of freezing injury | Freezing injury Rate (%) | |
| Example 1 | 0.19 | 5.5 |
| Example 2 | 0.22 | 6.0 |
| Comparative example 1 | 0.31 | 9.5 |
| Comparative example 2 | 0.35 | 11.5 |
| Comparative example 3 | 0.41 | 15.0 |
| Clear water control group | 0.53 | 21.5 |
As can be seen from Table 1, the seedling antifreeze of the embodiments 1 to 2 of the present invention has a lower freeze injury index and freeze injury rate than those of the comparative examples 1 to 3 and the clear water control group.
Furthermore, it should be understood that various changes, substitutions, deletions, modifications or adjustments may be made by those skilled in the art after reading the disclosure of the present invention, and such equivalents are also within the scope of the invention as defined in the appended claims.
Claims (10)
1. The nursery stock antifreezing solution is characterized in that the formula comprises L-hydroxyproline and calcium salt; advantageously, the content of L-hydroxyproline is between 40 and 80 mg/L.
2. The nursery stock antifreezing solution according to claim 1, wherein the content of the calcium salt is 700-900 mg/L.
3. The seedling antifreeze of claim 1, wherein the formulation further comprises spermidine.
4. The seedling antifreeze solution of claim 3, wherein the content of the spermidine is 20-40 mg/L.
5. The seedling antifreeze of claim 1, wherein the formulation further comprises jasmonic acid.
6. The seedling antifreeze solution of claim 5, wherein the jasmonic acid content is 10-30 mg/L.
7. The nursery stock antifreezing solution according to claim 1, wherein the formulation further comprises dirhamnolipid.
8. The nursery stock antifreezing solution according to claim 7, wherein the content of the dirhamnolipid is 80-110 mg/L.
9. The nursery stock antifreezing solution according to claim 1, wherein the formula is as follows:
50-60mg/L of L-hydroxyproline;
25-35mg/L of spermidine;
15-25mg/L jasmonic acid;
750 mg/L of calcium chloride and 850mg/L of calcium chloride;
the dual rhamnolipid accounts for 90-100 mg/L;
the balance being water.
10. The preparation method of the nursery stock anti-freezing solution according to claim 9, wherein the method comprises the following steps:
adding the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid into a proper amount of water according to the formula ratio, stirring to dissolve the L-hydroxyproline, the spermidine, the jasmonic acid, the calcium chloride and the dirhamnolipid, uniformly mixing, adding the rest water, and stirring to uniformly mix.
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| CN202210861562.XA CN115119844B (en) | 2022-07-20 | 2022-07-20 | Seedling antifreeze fluid and preparation method thereof |
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| CN202210861562.XA CN115119844B (en) | 2022-07-20 | 2022-07-20 | Seedling antifreeze fluid and preparation method thereof |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103027032A (en) * | 2012-09-29 | 2013-04-10 | 浙江大学 | Application of rhamnolipid serving as protective agent of low-temperature or ultralow-temperature cell preservation |
| CN106116829A (en) * | 2016-06-22 | 2016-11-16 | 武汉农尚环境股份有限公司 | A kind of freeze proof reagent of ornamental plant and compound method thereof |
| CN107223666A (en) * | 2017-06-27 | 2017-10-03 | 湖南省蔬菜研究所 | A kind of composition for improving chilli seedling winter resistance and its preparation method and application |
| CN108934544A (en) * | 2018-04-27 | 2018-12-07 | 河北省林业科学研究院 | A kind of nursery stock cold-resistant anti-freezing method |
| CN109924199A (en) * | 2017-12-16 | 2019-06-25 | 新昌县汉良苗木专业合作社 | A kind of nursery stock anti-icing fluid |
| CN111543575A (en) * | 2020-04-10 | 2020-08-18 | 浙江大学舟山海洋研究中心 | Antifreezing solution for freezing food |
-
2022
- 2022-07-20 CN CN202210861562.XA patent/CN115119844B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103027032A (en) * | 2012-09-29 | 2013-04-10 | 浙江大学 | Application of rhamnolipid serving as protective agent of low-temperature or ultralow-temperature cell preservation |
| CN106116829A (en) * | 2016-06-22 | 2016-11-16 | 武汉农尚环境股份有限公司 | A kind of freeze proof reagent of ornamental plant and compound method thereof |
| CN107223666A (en) * | 2017-06-27 | 2017-10-03 | 湖南省蔬菜研究所 | A kind of composition for improving chilli seedling winter resistance and its preparation method and application |
| CN109924199A (en) * | 2017-12-16 | 2019-06-25 | 新昌县汉良苗木专业合作社 | A kind of nursery stock anti-icing fluid |
| CN108934544A (en) * | 2018-04-27 | 2018-12-07 | 河北省林业科学研究院 | A kind of nursery stock cold-resistant anti-freezing method |
| CN111543575A (en) * | 2020-04-10 | 2020-08-18 | 浙江大学舟山海洋研究中心 | Antifreezing solution for freezing food |
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