CN111855633A - Rapid automatic accurate alignment system for steady-state transient fluorescence spectrum measurement technology and control method - Google Patents
Rapid automatic accurate alignment system for steady-state transient fluorescence spectrum measurement technology and control method Download PDFInfo
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- CN111855633A CN111855633A CN202010847658.1A CN202010847658A CN111855633A CN 111855633 A CN111855633 A CN 111855633A CN 202010847658 A CN202010847658 A CN 202010847658A CN 111855633 A CN111855633 A CN 111855633A
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000001052 transient effect Effects 0.000 title claims abstract description 18
- 238000002189 fluorescence spectrum Methods 0.000 title claims abstract description 11
- 238000005516 engineering process Methods 0.000 title claims abstract description 9
- 238000005259 measurement Methods 0.000 title abstract description 6
- 238000012360 testing method Methods 0.000 claims abstract description 28
- 238000006073 displacement reaction Methods 0.000 claims abstract description 27
- 238000001506 fluorescence spectroscopy Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 239000000843 powder Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 description 5
- 238000004891 communication Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000004677 Nylon Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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Abstract
A rapid automatic accurate alignment system and a control method for a steady-state transient fluorescence spectrum measurement technology belong to the technical field of fluorescence testing, and solve the problems that stepping cannot be accurately controlled and the precision is low in the testing process of a steady-state transient fluorescence spectrometer. The system comprises: the device comprises a signal sensor, a detector, a feedback and control unit, a stepping motor, a screw-nut pair, a displacement table and a sample table; the sample stage is arranged on the displacement stage, the displacement stage is driven by the stepping motor through the screw-nut pair to reciprocate along the screw, the detector collects voltage signals of fluorescence spectra of samples when the sample stage is at different positions, the voltage signals are read by the signal sensor and collected by the feedback and control unit, the feedback and control unit determines the strongest position of the voltage signals, the stepping motor is controlled, the sample stage is returned to the strongest position of the signals, and automatic alignment is completed. The invention realizes the quick, accurate and automatic alignment of the sample stage, saves the test time, improves the test efficiency and completes the test result with accurate and high signal-to-noise ratio.
Description
Technical Field
The invention belongs to the technical field of fluorescence testing, and particularly relates to a rapid automatic accurate alignment system and a control method for a steady-state transient fluorescence spectrum measurement technology.
Background
The photovoltaic material can be used for manufacturing various photoelectric devices such as photoelectric sensors, optical information processors, solar cells and the like by utilizing the photovoltaic effect; the luminescent material can be used for manufacturing light emitting diodes and the like, and has wide application in the fields of illumination and display. The steady-state transient fluorescence spectrum technology is characterized in that exciting light with certain intensity is utilized to excite a sample, and excited electrons in the sample can emit photons in the transition process from an excited state to a ground state. Therefore, the technology has very important significance for the performance evaluation of photovoltaic materials and luminescent materials.
At present, a sample holder of an existing steady-state transient fluorescence spectrometer can only be manually adjusted to reach an optimal position for excitation and fluorescence detection, but manual adjustment cannot accurately control stepping, the accuracy is low, and long adjustment time is wasted in the process of searching for a maximum signal; in addition, if the best focus position is not reached, the signal-to-noise ratio of the weak signal is poor, and the calculation result of the luminous life is influenced. With the increasing demand for testing and the increasing demand for testing accuracy, new testing systems are needed to solve this problem.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a rapid automatic precise alignment system and a control method for a steady-state transient fluorescence spectrum measurement technology, and solves the problems that stepping cannot be precisely controlled and the precision is low in the test process of a steady-state transient fluorescence spectrometer.
The technical scheme adopted by the invention for solving the technical problem is as follows:
a rapid automatic precise alignment system for steady state transient fluorescence spectroscopy techniques, the system comprising: the device comprises a signal sensor, a detector, a feedback and control unit, a stepping motor, a screw-nut pair, a displacement table and a sample table; the sample platform is arranged on the displacement platform, the displacement platform is driven by the stepping motor through the screw nut pair to reciprocate along the screw, the detector collects voltage signals of fluorescence spectra of samples when the sample platform is at different positions, the voltage signals are read by the signal sensor and collected by the feedback and control unit, the feedback and control unit determines the strongest position of the voltage signals to control the stepping motor, and the sample platform returns to the strongest position of the signals to complete automatic alignment.
Preferably, the detector, the signal sensor, the feedback and control unit, the stepping motor, the screw-nut pair and the displacement table are sequentially connected.
Preferably, the signal sensor and the detector are connected through an SMA/BNC tee joint.
Preferably, the other end of the SMA/BNC tee joint is connected with an oscilloscope or a multimeter.
Preferably, a limiting block for storing a liquid or solid film sample is arranged in the sample stage.
Preferably, the stepping motor is connected with the screw nut pair through a support with a bearing, and the displacement distance is 5-150 mm.
Preferably, the detector is a photomultiplier tube.
A method for controlling a fast automatic precise alignment system for steady state transient fluorescence spectroscopy, the method comprising the steps of:
the method comprises the following steps: the stepping motor drives the displacement table to reach a preset position according to the instruction of the feedback and control unit;
step two: the signal sensor acquires test data acquired by the detector in an SMA/BNC three-way bridging mode;
step three: the feedback and control unit reads the test data acquired by the signal sensor, records the data and records the position of the stepping motor; according to the instruction set by the feedback and control unit, the corresponding distance is advanced, the test data and the position of the stepping motor are recorded, and the process is repeated until the set instruction is finished;
step four: and after the feedback and control unit finishes data recording, judging the maximum value of the test data, and driving the sample stage to return to the corresponding position when the maximum value of the test data by the stepping motor to finish the automatic alignment function.
The invention has the beneficial effects that: the invention realizes the quick, accurate and automatic alignment of the sample stage. The test time is saved, the test efficiency is improved, and an accurate test result with high signal-to-noise ratio can be completed without operating personnel who is trained professionally.
Drawings
FIG. 1 is a schematic diagram of a fast automatic precise alignment system for steady-state transient fluorescence spectrometry.
FIG. 2 is a schematic diagram of a fast automatic precise alignment system for steady state transient fluorescence spectroscopy.
FIG. 3 is a side view of a fast automatic precise alignment system sample stage for steady state transient fluorescence spectroscopy.
In the figure, the device comprises a sample table, a feedback and control unit, a first SMA/BNC connecting line, a detector, a displacement table, a second SMA/BNC connecting line, a third SMA/BNC connecting line, a SMA/BNC tee joint, a signal sensor feedback control unit communication connecting line, a stepping motor and feedback control unit communication connecting line, a stepping motor, a support with a bearing, a lead screw, a limiting block and a positioning block, wherein the signal sensor is 1, the sample table is 2, the feedback and control unit is 3, the first SMA/.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
1-3, a rapid automatic precise alignment system for steady state transient fluorescence spectroscopy techniques, the system comprising: the device comprises a signal sensor 1, a detector 5, a feedback and control unit 3, a stepping motor 12, a screw-nut pair, a displacement table 6 and a sample table 2; detector 5, signal sensor 1, feedback and the control unit 3, step motor 12, screw nut is vice and displacement platform 6 are connected in order, promptly detector 5 with the one end of signal sensor 1 is connected, the other end of signal sensor 1 with the one end of feedback and the control unit 3 is connected, the other end of feedback and the control unit 3 with the one end of step motor 12 is connected, the other end of step motor 12 with the vice one end of screw nut is connected, the vice other end of screw nut with displacement platform 6 is connected. A limiting block 15 is arranged in the sample table 2, wherein samples such as liquid or solid films and powder for experiments are stored in the limiting block 15. The sample stage 2 is arranged on the displacement stage 6, the displacement stage 6 is driven by a stepping motor 12 through the screw-nut pair to reciprocate along a screw 14, the detector 5 collects voltage signals of fluorescence spectra of samples when the sample stage 2 is at different positions, the voltage signals are read by the signal sensor 1 through an SMA/BNC tee joint 9 and are collected by the feedback and control unit 3, wherein the detector 5 is connected with the SMA/BNC tee joint 9 through a third SMA/BNC connecting line 8, the SMA/BNC tee joint 9 is connected with the signal sensor 1 through a third SMA/BNC connecting line 4, and the signal sensor 1 is connected with the feedback and control unit 3 through a signal sensor feedback control unit communication connecting line 10; the feedback and control unit 3 collects and determines the strongest position of the voltage signal through the detector 5, controls the stepping motor 12, and drives the sample table 2 to return to the strongest position of the voltage signal through the screw-nut pair to complete automatic alignment. The nut is arranged at the bottom of the displacement table 6, and the displacement table 6 is driven to move to the position with the strongest signal through the rotation of the screw rod 14. The stepping motor 12 is connected with the screw nut pair through a support 13 with a bearing, and the position of the screw nut pair is fixed by the bearing. The detector 5 is selected as a photomultiplier.
a displacement table 6: control of three essences (brand), model: ETSM-50, stroke 50 mm.
Sample stage 2: the material is black nylon, and the chassis height is 10mm, and 4 diameter through the bottom are continuous with step motor displacement platform for 4 mm's installation screw, and the sample stopper is high 57 mm.
In order to better observe and read the voltage signal detected by the detector 5, one end of the SMA/BNC tee 9 is connected with an oscilloscope or a multimeter, and the oscilloscope or the multimeter is connected with the SMA female tee through a second SMA/BNC connecting line 74.
The control method of the rapid automatic accurate alignment system based on the steady-state transient fluorescence spectrum measurement technology comprises the following steps:
the method comprises the following steps: the stepping motor 12 drives the sample stage 2 to reach a preset position through the screw-nut pair according to the instruction of the feedback and control unit 3;
step two: the signal sensor 1 obtains the test data collected by the detector 5 in a mode of bridging the SMA/BNC tee joint 9;
step three: the feedback and control unit 3 reads the test data acquired by the signal sensor 1, records the current data and records the position of the stepping motor 12 at the moment; according to the instruction set by the feedback and control unit 3, the stepping motor 12 controls the displacement table 6 to advance by a corresponding distance, records the test data and the position of the sample table 2, and repeats the process until the set instruction is completed;
step four: after the feedback and control unit 3 finishes data recording, the maximum value of the test data is judged, and the stepping motor 12 drives the sample stage 2 to return to the corresponding position when the maximum value of the test data is reached, so that the automatic alignment function is finished.
Claims (8)
1. A rapid automatic precise alignment system for steady state transient fluorescence spectroscopy techniques, the system comprising: the device comprises a signal sensor, a detector, a feedback and control unit, a stepping motor, a screw-nut pair, a displacement table and a sample table; the sample platform is arranged on the displacement platform, the displacement platform is driven by the stepping motor through the screw nut pair to reciprocate along the screw, the detector collects voltage signals of fluorescence spectra of samples when the sample platform is at different positions, the voltage signals are read by the signal sensor and collected by the feedback and control unit, the feedback and control unit determines the strongest position of the voltage signals to control the stepping motor, and the sample platform returns to the strongest position of the signals to complete automatic alignment.
2. The system of claim 1, wherein the detector, the signal sensor, the feedback and control unit, the stepper motor, the screw-nut pair and the displacement stage are connected in sequence.
3. The system of claim 1, wherein the signal sensor and the detector are connected by an SMA/BNC tee.
4. The system of claim 3, wherein the other end of the SMA/BNC tee is connected to an oscilloscope or a multimeter.
5. The system of claim 1, wherein the sample stage comprises a stopper for storing a liquid, solid film or powder sample.
6. The system of claim 1, wherein the stepper motor is connected with the screw nut pair through a bearing, and the displacement distance is 5-150 mm.
7. The system of claim 1, wherein the detector is a photomultiplier tube.
8. The method for controlling the fast automatic precise alignment system for the steady-state transient fluorescence spectrometry technology according to claims 1-7, wherein the method comprises the following steps:
the method comprises the following steps: the stepping motor drives the displacement table to reach a preset position according to the instruction of the feedback and control unit;
step two: the signal sensor acquires test data acquired by the detector in an SMA/BNC three-way bridging mode;
step three: the feedback and control unit reads the test data acquired by the signal sensor, records the data and records the position of the stepping motor; according to the instruction set by the feedback and control unit, the corresponding distance is advanced, the test data and the position of the stepping motor are recorded, and the process is repeated until the set instruction is finished;
step four: and after the feedback and control unit finishes data recording, judging the maximum value of the test data, and driving the sample stage to return to the corresponding position when the maximum value of the test data by the stepping motor to finish the automatic alignment function.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010847658.1A CN111855633A (en) | 2020-08-21 | 2020-08-21 | Rapid automatic accurate alignment system for steady-state transient fluorescence spectrum measurement technology and control method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113075175A (en) * | 2021-03-15 | 2021-07-06 | 中国科学院福建物质结构研究所 | Broadband time-resolved fluorescence immunoassay device and analysis method |
| CN113466196A (en) * | 2021-06-29 | 2021-10-01 | 中国科学院长春应用化学研究所 | Multi-position sample stage adaptive to steady-state transient fluorescence spectrometer and using method thereof |
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