CN204065094U - A kind of continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument - Google Patents

A kind of continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument Download PDF

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Publication number
CN204065094U
CN204065094U CN201420381330.5U CN201420381330U CN204065094U CN 204065094 U CN204065094 U CN 204065094U CN 201420381330 U CN201420381330 U CN 201420381330U CN 204065094 U CN204065094 U CN 204065094U
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China
Prior art keywords
sample introduction
synchronizing wheel
guide rail
module
driven synchronizing
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Expired - Lifetime
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CN201420381330.5U
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Chinese (zh)
Inventor
张国锋
张含
兰亮明
周洋
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Xiamen Baotai Biotechnology Co ltd
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XIAMEN BAOTAI BIOTECHNOLOGY CO Ltd
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  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The utility model relates to the detecting instrument in chemiluminescence field, particularly relates to a kind of continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument.This fluorescence analyser comprises: Scanning Detction module, bar code scan module, bar shaped sample drive assembly, go out sample drawer and sample introduction roller, wherein Scanning Detction module comprises again excitation-detection module, photoelectric conversion module, control analysis module, and Scanning Detction module is positioned at the top of bar shaped sample drive assembly; Bar shaped sample drive assembly comprises again Timing Belt, the first guide rail, the second guide rail, active synchronization wheel, driven synchronizing wheel, motor, sample introduction plate and support baseboard; Go out sample drawer and be positioned at the second guide rail side of bar shaped sample drive assembly and near driven synchronizing wheel, the side that sample introduction roller is positioned at the second guide rail is connected with sample introduction tank and for carrying reagent strip to be detected to sample introduction tank.This continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument is mainly used in chemiluminescence field and detects.

Description

A kind of continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument
Technical field
The utility model relates to the detecting instrument in chemiluminescence field, particularly relates to a kind of continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument.
Background technology
Chemiluminescence analysis system utilizes the catalysis of chemiluminescent substance through catalyzer and the oxidation of oxygenant, form the intermediate of an excited state, when this excited state intermediate gets back to stable ground state, launch photon simultaneously, utilize luminous signal surveying instrument to measure a kind of analytical approach of quantum yield of luminscence.Immunofluorence technic belongs to chemiluminescence analysis system, but due to problems such as the background in general fluorometric assay are higher, immunofluorence technic is used for quantitative measurement certain difficulty.Several special fluorescence immunoassay technology of development in recent years, such as fluorescent latex mark chromatography detection technique, this technology is as a kind of immunological method, it is the combination of immune affine technology, engram technology, immunolabelling technique and chromatographic technique, and utilizes chemiluminescence analysis automatic detection device to detect fluorescence signal.This technology have quick, easy and simple to handle, stable reagent, can the feature such as room temperature accumulating, not easily pollution, but the Time-resolved Fluoroimmunoassay instrument that relies on of this technology is all carry out sampling and testing for the detection great majority of bar shaped sample artificial to be added in sample storehouse by sample at present, because can not continuous sample-adding, cause the problem that testing efficiency is low.Even if having a few devices to have the automatic transmission assembly of bar shaped sample at present, but also there is load position inconvenience, Sample location precision is low waits deficiency.
As can be seen here, structurally also there is certain defect and have much room for improvement in above-mentioned existing Time-resolved Fluoroimmunoassay instrument.
Summary of the invention
For the problems referred to above, the utility model proposes a kind of high-precision continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument, for solve current Time-resolved Fluoroimmunoassay instrument stability and sensitivity low, can not continuous sample introduction, and to detecting the more difficult problem of Sample location.
The technical solution of the utility model is:
A kind of continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument comprises: Instrument shell and be arranged at Scanning Detction module, bar code scan module, bar shaped sample drive assembly in this Instrument shell, go out sample drawer and sample introduction roller; It is characterized in that: described sample introduction roller is mounted on bar shaped sample drive assembly transmission entrance, Scanning Detction module and bar code scan module are arranged at the two ends of the top of bar shaped sample drive assembly respectively, described in go out the transmission exit lower position that sample drawer is mounted on this bar shaped sample drive assembly.Preferably, this excitation-detection module comprises again pedestal, LED light source, radiating circuit plate, convex lens, slit assembly, optical filter, scanner head, sweep circuit plate and anti-dazzling screen, described LED light source and pedestal is angled is fixed in the mounting hole of pedestal, and point to described testing sample detection zone as excitation source, and described LED light source can launch the exciting light in visible wavelength range; Described convex lens are arranged on described LED light source front.Described slit assembly is arranged on the central part in base bottom, and the exciting light that described LED light source is launched can focus on and concentrate on surface, described testing sample detection zone by described convex lens and described slit assembly; Anti-dazzling screen is positioned at the bottom of scan subject pedestal, for the volume reflection of the exposure and reflected light of correcting laser, light on sample filters out the optical filter again through being arranged on above described slit assembly after unnecessary light through anti-dazzling screen, the parasitic light in the reflected light after the fluorescence probe for marking in filtering testing sample is stimulated; Described scanner head is fixed on the top of optical filter, also prepares this reflected light signal to be converted into electric signal further for receiving the fluorescence probe reflected light obtained after described filtering assembly filtering parasitic light.
Bar shaped sample drive assembly comprises again Timing Belt, the first guide rail, the second guide rail, active synchronization wheel, driven synchronizing wheel, motor and support baseboard composition.The erection parallel with described driven synchronizing wheel of wherein said active synchronization wheel is fixed on support baseboard, and the outside surface of described Timing Belt is provided with equally spaced blend stop, and be set in active synchronization take turns and driven synchronizing wheel outside surface on form closing structure.Described first guide rail, the second guide rail and Timing Belt are positioned at same level and are positioned over the both sides of Timing Belt, and are fixed on the support of active synchronization wheel and driven synchronizing wheel; The side that described first guide rail is relative with the second guide rail all offers the bevelled draw-in groove of band, and the second guide rail is taken turns one end near active synchronization and offered sample introduction tank, and described guide rail is also provided with the hyperchannel breach for releasing sample near the side of driven synchronizing wheel.Further this driven synchronizing wheel is also provided with driven synchronizing wheel regulating device, regulates driven synchronizing wheel regulating device can reach the effect of setting tightness of synchronous belt.Go out sample drawer and be positioned at the second guide rail side of bar shaped sample drive assembly and near driven synchronizing wheel, the side that sample introduction roller is positioned at the second guide rail is connected with sample introduction tank and for carrying reagent strip to be detected to sample introduction tank.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the structural representation of the utility model excitation-detection module.
Fig. 3 is the structural representation of the utility model bar shaped sample drive assembly.
Fig. 4 a is the first guide rail of the utility model bar shaped sample drive assembly.
Fig. 4 b is the second guide rail of the utility model bar shaped sample drive assembly.
Embodiment
Now the utility model is described in further detail with embodiment by reference to the accompanying drawings.
Consult shown in Fig. 1, continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument of the present utility model comprises: Instrument shell and be arranged at Scanning Detction module 1, bar code scan module 2, bar shaped sample drive assembly 3 in this Instrument shell, go out sample drawer 4 and sample introduction roller 5.Described sample introduction roller 5 is mounted on bar shaped sample drive assembly 3 and transmits entrance, transmit for bar shaped sample (test card) is delivered on bar shaped sample drive assembly 3, wherein Scanning Detction module 1 is integrated with again excitation-detection module, photoelectric conversion module, control analysis module, Scanning Detction module 1 and bar code scan module 2 are arranged at the two ends of the top of bar shaped sample drive assembly 3 (preferably respectively, bar code scan module 2 and Scanning Detction module 1 is set gradually) according in the transmission direction of bar shaped sample drive assembly 3, be respectively used to the barcode size or text field in successively scanning bar shaped sample (test card) and fluorescing measuring fields, described go out sample drawer 4 be mounted on the transmission exit lower position of this bar shaped sample drive assembly 3, for reclaiming the bar shaped sample (test card) after Scanning Detction.
Consult shown in Fig. 2, described Scanning Detction module 1 comprises pedestal 11, radiating circuit plate 12, LED light source 13, convex lens 14, slit assembly 15, O type packing ring 16, optical filter 17, scanner head 18, sweep circuit plate 181 and anti-dazzling screen 19, two inclined-planes of pedestal 11 being respectively arranged with xsect is circular symmetrical through hole 112, the axle center of through hole 112 and the angle of pedestal 11 bottom surface are between 30-90 degree, and its diameter and LED light source 13 match; Radiating circuit plate 12 is connected to the rear of LED light source 13, and convex lens 14 are positioned at the front of LED light source, and LED light source is inserted in through hole 112, and fit in the inclined-plane in outside and pedestal 11 that radiating circuit plate 12 is positioned at through hole and available screw is fixed on the inclined-plane of pedestal 11.The centre of pedestal 11 end face offers through hole 113, and anti-dazzling screen 19 laminating is the bottom of pedestal 11 and is positioned at below through hole 113 and covers through hole 113; Position near anti-dazzling screen 19 in through hole 113 is provided with slit assembly 15, and optical filter 17 is positioned on slit assembly 15, and scanner head 18 is positioned in through hole, and is positioned at the top of optical filter 17, and can place O type packing ring 16 bottom scanner head 18.One end away from through hole 112 on the inclined-plane of pedestal 11 both sides offers through hole 114 respectively, two sweep circuit plates 181 offers respectively three corresponding between two through holes 1811,1812,1813, and in three groups of through holes, is inserted with three bolts respectively; Bolt wherein in through hole 1811 is by fastened to each other for two sweep circuit plates, bolt in through hole 1812 and 1813 matches with through hole 114 for two sweep circuit plates 181 are fixed on pedestal, and two sweep circuit plates 181 are connected for driving scanner head with scanner head 18.
Consult shown in Fig. 3 and Fig. 4 a and Fig. 4 b, the bar shaped sample drive assembly 3 of chemiluminescence field pick-up unit comprises Timing Belt 301, first guide rail 302, second guide rail 303, active synchronization wheel 304, driven synchronizing wheel 305, motor 306, and support baseboard 308 forms.Wherein said active synchronization wheel 304 includes synchronizing wheel main body and synchronous wheel shaft two parts with described driven synchronizing wheel 305, and synchronous wheel shaft is all positioned at the shaft core position of synchronizing wheel main body; Wherein active synchronization wheel 304 is fixed on active synchronization wheel support 3042 by active synchronization wheel shaft 3041, preferably, active synchronization wheel support 3042 is two and all offer axis hole, the two ends of active synchronization wheel shaft 3041 all stretch out active synchronization wheel 304 main body and to be inserted into respectively in two axis holes of active synchronization wheel support 3042 and and active synchronization wheel support 3042 be fixed.Described at driven synchronizing wheel 305 except comprising synchronizing wheel main body and synchronous wheel shaft two parts, also comprise two driven synchronizing wheel regulating shafts 3052, the two ends of driven synchronous wheel shaft 3051 all have threaded hole with axially vertical position, the outside of two driven synchronizing wheel regulating shafts 3052 is all processed with the external thread corresponding with the threaded hole of driven synchronous wheel shaft 3051, and two driven synchronizing wheel regulating shafts 3052 become 90 degree of two ends screwing in driven synchronous wheel shaft 3051 respectively with the two ends of driven synchronous wheel shaft 3051; Described two driven synchronizing wheel supports 3053 offer two symmetrical threaded holes corresponding with the external thread of driven synchronizing wheel regulating shaft 3052 respectively, are used for fixing driven synchronizing wheel regulating shaft 3052 by nut.Active synchronization wheel 304 and driven synchronizing wheel 305 are fixed on support baseboard 309 respectively by synchronizing wheel support 3042,3053 erections parallel to each other; Timing Belt 301 be set in active synchronization wheel and driven synchronizing wheel outside surface on form closing structure, the outside surface of described Timing Belt 301 is fixed with blend stop according to the width of sample is equally spaced, and blend stop is parallel to each other and vertical with the direction of motion of Timing Belt.Unclamp the nut of driven synchronizing wheel regulating shaft 3052, regulate the position of the driven synchronizing wheel regulating shaft 3052 at two ends to make driven synchronizing wheel 305 vertically moving thus adjusting the degree of tightness of Timing Belt 301 along Timing Belt 301 simultaneously.
Timing Belt 301 is close to and parallel with the upper surface of Timing Belt 301 in the both sides that described first guide rail 302 and the second guide rail 303 lay respectively at Timing Belt 301, first guide rail 302 and the second guide rail 303 offer through hole respectively, support bar to be fixed on support baseboard 308 and to insert this through hole and the first guide rail 302 and the second guide rail 303 to be fixed, and higher than support baseboard 308 surface, fixed mount is located on support baseboard 308 respectively to make two guide rails.The side that first guide rail 302 is relative with the second guide rail 303 is processed with locating slot 3021 and 3031 respectively, locating slot is divided into again stop part 30211 and 30212 and adjustment part 30311 and 30312, stop part 30211 and 30212 upper and lower surface all and guide rail parallel, for limiting the position of sample, stop part about 30211 and 30,212 two planes extend and then form adjustment part 30311 and 30312 respectively to the angle that tilted upward is certain, and the base of this adjustment part engages with Timing Belt 301.Second guide rail 303 is taken turns 304 one end near active synchronization and is also offered sample introduction tank 3033, sample from then on sample introduction tank 3033 advances and is fastened between two blend stops of Timing Belt 301, as in the process pushing sample, there is sample and cross the phenomenon pushed away, sample will be blocked by the stop part of the locating slot 3021 of the first guide rail 302, and slides into the assigned address of Timing Belt 301 by the adjustment part of this locating slot 3021 and continue to follow Timing Belt motion.Described second guide rail 303 is also provided with hyperchannel breach 3032 near the side of driven synchronizing wheel, for releasing sample.Motor 306 is positioned at the side of active synchronization wheel 304 and is connected with the synchronous wheel shaft 3041 that active synchronization takes turns 304.
When this continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument works, the motor 306 of described bar shaped sample drive assembly 3 is positioned at the side of active synchronization wheel 304 for driving active synchronization wheel 304, sample is joined the centre of two blend stops of travelling belt 301 and by the bevelled draw-in groove adjustment of the band inside the first guide rail 302 and the second guide rail 303 lateral attitude, adjusts rear sample and follow Timing Belt 301 to move to driven synchronizing wheel 305 direction by sample through sample introduction roller 5.When reagent strip is through excitation-detection module 1, excitation-detection module 1 sends excitation beam to the detection zone at reagent strip place, the fluorescence signal of reagent strip reflection converts electric signal to by photoelectric conversion module, control analysis module comprises main circuit again, motor, display screen, electric signal is outputted on outside display instrument by the analysis of control analysis module again.
Although specifically show in conjunction with preferred embodiment and describe the utility model; but those skilled in the art should be understood that; not departing from the spirit and scope of the present utility model that appended claims limits; can make a variety of changes the utility model in the form and details, be protection domain of the present utility model.

Claims (6)

1. a continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument, comprising: Instrument shell and be arranged at Scanning Detction module, bar code scan module, bar shaped sample drive assembly in this Instrument shell, go out sample drawer and sample introduction roller, it is characterized in that: described sample introduction roller is mounted on bar shaped sample drive assembly transmission entrance, Scanning Detction module and bar code scan module are arranged at the two ends of the top of bar shaped sample drive assembly respectively, described in go out the transmission exit lower position that sample drawer is mounted on this bar shaped sample drive assembly, wherein, Scanning Detction module is integrated with again excitation-detection module, photoelectric conversion module, control analysis module, bar shaped sample drive assembly comprises Timing Belt, the first guide rail, the second guide rail, active synchronization wheel, driven synchronizing wheel, motor, sample introduction plate and support baseboard, the erection parallel with described driven synchronizing wheel of wherein said active synchronization wheel is fixed on support baseboard, parallelly on the outside surface of described Timing Belt be fixed with equally spaced blend stop, and the outside surface being set in active synchronization wheel and driven synchronizing wheel forms closing structure, described first guide rail, second guide rail and Timing Belt are positioned at same level and are positioned over the both sides of Timing Belt, and be fixed on support baseboard, second guide rail is taken turns one end near active synchronization and is offered sample introduction tank, and be also provided with the hyperchannel breach for releasing sample near the side of driven synchronizing wheel, the side that first guide rail is relative with the second guide rail is all processed with locating slot, this locating slot comprises stop part and adjustment part, stop part upper and lower surface all and guide rail parallel, upper and lower two planes of stop part extend and then form adjustment part respectively to the angle that tilted upward is certain, the base of this adjustment part engages with Timing Belt, the side that described motor is positioned at active synchronization wheel is taken turns for driving active synchronization.
2. continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument according to claim 1, it is characterized in that: Scanning Detction module also comprises pedestal, radiating circuit plate, LED light source, convex lens, slit assembly, optical filter, scanner head, sweep circuit plate and anti-dazzling screen, described pedestal has through hole, and the bottom that described anti-dazzling screen fits in pedestal to be positioned at below through hole and to cover through hole; Position near anti-dazzling screen in through hole is provided with slit assembly, and scanner head is positioned in through hole, and is positioned at the top of slit assembly, and the top that two sweep circuit plates are positioned at scanner head is connected with scanner head, and is fixed on pedestal; The angled base interior that is fixed in the bottom surface of described LED light source and pedestal points to anti-dazzling screen as excitation source, and radiating circuit plate is connected to the rear of LED light source, and described convex lens are arranged on described LED light source front.
3. continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument according to claim 2, is characterized in that: the angle of LED light source and base bottom surface is between 30-90 degree.
4. according to described continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument arbitrary in claim 1-3, it is characterized in that: also comprise active synchronization wheel support and driven synchronizing wheel support, active synchronization wheel forms by synchronizing wheel main body and two, synchronous wheel shaft part with driven synchronizing wheel, and wherein active synchronization wheel is fixed on active synchronization wheel support by active synchronization wheel shaft; Driven synchronizing wheel is fixed on driven synchronizing wheel support by driven synchronous wheel shaft.
5. continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument according to claim 4, it is characterized in that: also comprise driven synchronizing wheel regulating shaft, the main body of driven synchronizing wheel is stretched out at the two ends of driven synchronous wheel shaft respectively, its two ends all have threaded hole, the outside of driven synchronizing wheel regulating shaft is all processed with the external thread corresponding with the threaded hole of driven synchronous wheel shaft, and driven synchronizing wheel regulating shaft becomes 90 degree to screw in driven synchronous wheel shaft with the two ends of driven synchronous wheel shaft; Described driven synchronizing wheel support offers two symmetrical threaded holes corresponding with the external thread of driven synchronizing wheel regulating shaft respectively, is used for fixing driven synchronizing wheel regulating shaft by nut.
6. continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument according to claim 1, is characterized in that: according in the transmission direction of bar shaped sample drive assembly, set gradually described bar code scan module and Scanning Detction module.
CN201420381330.5U 2014-07-11 2014-07-11 A kind of continuous sample introduction formula Time-resolved Fluoroimmunoassay instrument Expired - Lifetime CN204065094U (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104655863A (en) * 2015-02-10 2015-05-27 中国科学院苏州生物医学工程技术研究所 Sample conveying detection system
CN105866077A (en) * 2016-03-21 2016-08-17 广东顺德工业设计研究院(广东顺德创新设计研究院) Time-resolved fluorescence analysis system and detection apparatus
CN105947645A (en) * 2016-07-15 2016-09-21 陶少强 Biosensor detection system and method
CN106124785A (en) * 2016-07-15 2016-11-16 陶少强 A kind of biosensor arrangement utilizing laser technology and control method thereof
CN107037025A (en) * 2017-06-24 2017-08-11 杭州微瑞科技有限公司 The high quick fluorescent chromatographic device of group of the lanthanides and detection method
CN107490674A (en) * 2017-08-02 2017-12-19 南京岚煜生物科技有限公司 A kind of Immunofluorescence test device and detection method
CN109738403A (en) * 2019-01-03 2019-05-10 必欧瀚生物技术(合肥)有限公司 A kind of preparation method of fluorescence standard card and fluorescence standard card fluorescent film
CN117286018A (en) * 2023-11-27 2023-12-26 珠海美华医疗科技有限公司 Drug sensitivity analysis system based on microorganism detection

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104655863A (en) * 2015-02-10 2015-05-27 中国科学院苏州生物医学工程技术研究所 Sample conveying detection system
CN105866077A (en) * 2016-03-21 2016-08-17 广东顺德工业设计研究院(广东顺德创新设计研究院) Time-resolved fluorescence analysis system and detection apparatus
CN105947645A (en) * 2016-07-15 2016-09-21 陶少强 Biosensor detection system and method
CN106124785A (en) * 2016-07-15 2016-11-16 陶少强 A kind of biosensor arrangement utilizing laser technology and control method thereof
CN105947645B (en) * 2016-07-15 2017-12-19 陶少强 A kind of biosensor detection system
CN106124785B (en) * 2016-07-15 2018-06-26 安徽国科生物科技有限公司 A kind of biosensor arrangement and its control method using laser technology
CN107037025A (en) * 2017-06-24 2017-08-11 杭州微瑞科技有限公司 The high quick fluorescent chromatographic device of group of the lanthanides and detection method
CN107490674A (en) * 2017-08-02 2017-12-19 南京岚煜生物科技有限公司 A kind of Immunofluorescence test device and detection method
CN109738403A (en) * 2019-01-03 2019-05-10 必欧瀚生物技术(合肥)有限公司 A kind of preparation method of fluorescence standard card and fluorescence standard card fluorescent film
CN117286018A (en) * 2023-11-27 2023-12-26 珠海美华医疗科技有限公司 Drug sensitivity analysis system based on microorganism detection
CN117286018B (en) * 2023-11-27 2024-02-20 珠海美华医疗科技有限公司 Drug sensitivity analysis system based on microorganism detection

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Granted publication date: 20141231