CN111802016A - Method for accelerating germination of tobacco seeds - Google Patents
Method for accelerating germination of tobacco seeds Download PDFInfo
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- CN111802016A CN111802016A CN202010638531.9A CN202010638531A CN111802016A CN 111802016 A CN111802016 A CN 111802016A CN 202010638531 A CN202010638531 A CN 202010638531A CN 111802016 A CN111802016 A CN 111802016A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Soil Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a method for accelerating germination of tobacco seeds, which comprises the following specific steps: sterilizing, and treating with 10% NaClO for 30 minutes; soaking seeds for the first time, wherein 100mg/L GA and 100g/L anion powder are respectively selected as germination accelerating agents, and the germination accelerating time is 2 days, namely 48 hours; accelerating germination and drying again, setting a time gradient for accelerating germination time, soaking seeds for the second time, wherein the method is the same as the first time, the two accelerating germination processes are all carried out under the condition of 25 ℃, the design is ingenious, the accelerating germination efficiency is obviously improved while the environment is protected, and the method is convenient to popularize, so that the method has high market application value.
Description
Technical Field
The invention relates to the technical field of tobacco planting, in particular to a method for accelerating germination of tobacco seeds.
Background
With the development of the technology, tobacco planting also tends to be scientific, technological and modern day by day.
In the prior art, the germination accelerating technological process is generally as follows:
(1) and (3) disinfection: sterilizing with 1% copper sulfate;
(2) soaking seeds for the first time: adding 100mg/L gibberellin into tobacco seeds (seeds are completely submerged in water);
(3) accelerating germination and drying: accelerating germination for 48 hours, drying and accelerating germination for 24 hours;
(4) and (4) soaking seeds for the second time, wherein the method is the same as that for the first time.
Problems in the prior art
(1) And (3) disinfection: 1% of copper sulfate used for disinfection, copper ions belong to heavy metal ions, certain residue may exist, pollution is caused to the environment, and the copper sulfate has strong toxicity and has certain toxicity to seeds.
(2) A germination accelerating agent: and (3) carrying out uniform concentration germination accelerating agent treatment (100mg/L GA) on all varieties, wherein the germination accelerating effect of the GA on the seeds is not ideal, the vitality retention time of the seeds after GA germination accelerating is short, and meanwhile, the characteristic difference among the varieties is ignored.
(3) Accelerating germination time: the unified pregermination time treatment is carried out on all varieties, and the characteristics of the varieties and the physiological characteristics of the seeds are ignored. The seed soaking time is long as the seed quantity is increased, and the germination rate of the seeds is obviously reduced.
(4) Drying temperature and time: too high a first redrying temperature (45 ℃) causes the seeds to lose water too quickly, possibly reducing seed vigor.
Disclosure of Invention
The invention provides a method for accelerating germination of tobacco seeds, which aims to solve the problems mentioned in the technical background, and the technical scheme of the invention is as follows:
a method for accelerating germination of tobacco seeds comprises the following specific steps:
(1) and (3) disinfection: treating with 10% NaClO for 30 min;
(2) soaking seeds for the first time: respectively selecting 100mg/L GA and 100g/L anion powder as germination accelerating agents; an oxygenator is required to be placed in the basin, or the tobacco seeds are manually stirred once every 2 hours, the germination accelerating agent is added into the tobacco seeds, so that water completely submerges the seeds, wherein the ratio of water: the weight ratio of the seeds is 1.5:1, and the germination accelerating time is 2 days, namely 48 hours;
(3) accelerating germination and drying: setting time gradient for germination accelerating time, controlling drying temperature below 45 deg.C, drying for the first time at 30 deg.C for 1h, and controlling water content of seed at 6.5-7%;
(4) the second seed soaking step is the same as the first step, wherein the germination accelerating time is 1 day, namely 24 hours, and the two germination accelerating processes are carried out at 25 ℃.
In the above technical scheme, the setting of the time gradient for the germination accelerating time is as follows:
advantageous effects
By adopting the scheme, the tobacco seed germination accelerating method provided by the invention is ingenious in design, obvious in germination accelerating efficiency while protecting the environment, and convenient to popularize, so that the tobacco seed germination accelerating method has high market application value.
Detailed Description
In order to facilitate an understanding of the present invention, the present invention is described in more detail below. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Examples
1 Disinfection
Pouring the selected seeds into a large basin, preparing sodium hypochlorite solution with the effective chlorine content of more than 5% into 10% sodium hypochlorite solution, adding the 10% sodium hypochlorite solution into the basin, timing for 30 minutes by a timer, then fishing out the seeds, using clean water for several times for no less than 5 minutes each time until the clean water does not change color, and finally washing twice by using distilled water.
2 first pregermination
Preparing gibberellin with the concentration of more than 90 percent to prepare a gibberellin solution with the concentration of 100mg/L, (1g of gibberellin is dissolved in 10 kg of water), mixing the sterilized seeds according to the weight ratio of the gibberellin solution to the seeds of 2:1, uniformly stirring, and then putting an oxygenator into a large basin to fill oxygen into the solution without fermenting. The germination accelerating time is 24 hours, and then the seeds are fished out and washed with clean water for two to three times.
3 go back to dry
And (3) drying the washed seeds again, drying the seeds in the sun or drying the seeds in an oven, wherein the drying temperature is controlled below 40 ℃. Sun exposure has the effect of breaking seed dormancy, so sun exposure to dryness is recommended. The water content of the seeds after drying is controlled to be about 10 percent.
As a preferred embodiment of the above technical solution, the method of the present invention comprises coating the selected seeds after applying the method, specifically as follows:
improved scheme of coating seeds
In the scheme, the new material in the sprouting scheme of Xiangyan No. 5 is anion powder; old material refers to gibberellin. In the scheme of GX-8, the new material is as follows: ABO type powder, the old material is: talcum powder combination agent.
The first germination accelerating scheme of Xiangyan No. 5 is as follows: the gibberellin is dried back and sprouted for 24 hours after pregermination for 24 hours,
the second germination accelerating scheme of Xiangyan No. 5 is as follows: the negative ion powder is used for accelerating germination for 48 hours and then is dried again for accelerating germination for 24 hours,
the first pregermination scheme of GX-8 is as follows: accelerating germination for 48 hours by using anion powder, drying again, accelerating germination for 12 hours,
the second pregermination scheme of GX-8 is as follows: the gibberellin is sprouted for 24 hours and then dried and sprouted for 24 hours, and the old germination accelerating scheme is the original germination accelerating scheme in the prior art mentioned in the application.
And (3) performing subsequent experimental determination on the coated seeds, performing a standard germination test, an aging test and a low-temperature test, determining the germination rate, the water content, the single seed rate, the cracking degree, the compression strength compounding degree and other clear coated seed determination indexes of the coated seeds, and determining the performance of the tobacco seeds in normal-temperature, artificial accelerated aging and low-temperature environments.
And (3) standard germination experiments, performing standard germination on the coated seeds obtained in the table, taking two layers of filter paper, flatly spreading the two layers of filter paper in a glass culture dish with the diameter of 9cm, fully absorbing water, respectively and uniformly placing the seeds and the contrast treated in the experiment on the filter paper, placing 100 seeds in each dish at the temperature of 25 ℃ into an illumination incubator for culture and germination, and respectively adding a proper amount of distilled water every day in each treatment to ensure normal growth.
Aging test, aging the coated seeds obtained from the above table at 40 deg.C for 1, 2, and 3 days, and performing standard germination test to determine related indexes.
In the low-temperature germination experiment, the coating seeds obtained in the table are grown at 16 ℃ for 7 days, then are transferred to 24 ℃ for 7 days, and then relevant indexes are determined.
Test results
(1) Xiangyan No. 5 standard germination test
As for the germination characteristic indexes, the four treatment differences of the germination rate and the germination potential are not obvious, and the germination rate and the germination potential can reach about 95 percent; the difference between the four treatments of dry weight and shoot length was also not significant. The four treatments also did not differ significantly in viability index and germination index in terms of viability index, but the mean germination time of Xiangyan No. 5 old protocol + old treatment was longer than the other three treatments.
(2) GX-8 Standard Germination test
As for the germination characteristic index, four processing differences of germination rate, germination vigor, seedling length and dry weight are not obvious; in terms of the viability index, there was no significant difference between the germination index, viability index and average germination time.
(3) Xiang tobacco No. 5 aging test
As for the germination characteristic index, the germination rate and the germination potential of the new material in the germination accelerating scheme 1+ and the new material in the germination accelerating scheme 2+ after aging for 2 days are remarkably different from those of the original scheme + the new material and the original scheme + the old material. For the vitality index, after aging for 2 days, the germination accelerating scheme 1+ the new material achieves the level of obvious difference in germination index and average germination days compared with the original scheme + the old material; the germination accelerating scheme 2+ the new material reaches a level of obvious difference in germination index compared with the original scheme + the old material.
(4) GX-8 aging test
Regarding the germination characteristic indexes, the germination rate and the germination potential of the new material in the germination accelerating scheme 1+ and the new material in the germination accelerating scheme 2+ after aging for 1, 2 and 3 days are obviously different from those of the original scheme and the old material. In terms of vitality indexes, after aging for 2 days, the germination indexes, vitality indexes and average germination days of the new material in the germination scheme 1+ and the new material in the germination scheme 2+ are all obviously superior to those of the old material in the original scheme, but have no obvious difference from the old material in the old scheme.
Therefore, according to the germination characteristic index and the activity index, for Xiangyan No. 5, a new material of a germination accelerating scheme 1+ is recommended; for GX-8, the selection of a germination protocol 1+ new material is recommended.
(5) Xiangyan No. 5 low-temperature test
Under the condition of low temperature (16 ℃), part of indexes cannot be normally counted, such as germination potential, due to slow germination process of seeds. In terms of germination characteristic indexes, the germination accelerating scheme 1+ new material and the germination accelerating scheme 2+ new material germination rate, older bud length scheme + old material have significant differences. In terms of vitality indexes, the germination scheme 1+ the new material and the germination scheme 2+ the germination index of the new material, the average germination days are significantly different from the old scheme + the old material.
(6) GX-8 Low temperature test
Under the condition of low temperature (16 ℃), the germination characteristic indexes of the new material and the germination accelerating scheme 1+ and the new material and the germination rate, the bud length and the dry weight of the old material and the old material have obvious difference. In terms of vitality indexes, the germination scheme 1+ the new material and the germination scheme 2+ the new material have a significantly different germination index and average germination time than the old scheme + the old material; germination accelerating scheme 1+ the vitality index of the new material is significantly different from the vitality index of the old scheme + the old material.
Therefore, according to the germination characteristic index and the activity index, for Xiangyan No. 5, a new material in a germination accelerating scheme 1+ and a new material in a germination accelerating scheme 2+ can be selected; for GX-8, the sprouting protocol 1+ new material is recommended. The low-temperature test and the aging test are basically consistent.
The technical features mentioned above are combined with each other to form various embodiments which are not listed above, and all of them are regarded as the scope of the present invention described in the specification; also, modifications and variations may be suggested to those skilled in the art in light of the above teachings, and it is intended to cover all such modifications and variations as fall within the true spirit and scope of the invention as defined by the appended claims.
Claims (2)
1. A tobacco seed germination accelerating method is characterized by comprising the following specific steps:
and (3) disinfection: treating with 10% NaClO for 30 min;
(2) soaking seeds for the first time: respectively selecting 100mg/L GA and 100g/L anion powder as germination accelerating agents; an oxygenator is required to be placed in the basin, or the tobacco seeds are manually stirred once every 2 hours, the germination accelerating agent is added into the tobacco seeds, so that water completely submerges the seeds, wherein the ratio of water: the weight ratio of the seeds is 1.5:1, and the germination accelerating time is 2 days, namely 48 hours;
(3) accelerating germination and drying: setting time gradient for germination accelerating time, controlling drying temperature below 45 deg.C, drying for the first time at 30 deg.C for 1h, and controlling water content of seed at 6.5-7%;
(4) and the second seed soaking step is the same as the first step, wherein the germination accelerating time is 1 day, namely 24 hours, and the two germination accelerating processes are carried out at 25 ℃.
2. The method for accelerating germination of tobacco seeds according to claim 1, wherein a time gradient is set for the accelerating germination time, and the method comprises the following steps:
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010638531.9A CN111802016A (en) | 2020-07-06 | 2020-07-06 | Method for accelerating germination of tobacco seeds |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010638531.9A CN111802016A (en) | 2020-07-06 | 2020-07-06 | Method for accelerating germination of tobacco seeds |
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| CN111802016A true CN111802016A (en) | 2020-10-23 |
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| CN202010638531.9A Pending CN111802016A (en) | 2020-07-06 | 2020-07-06 | Method for accelerating germination of tobacco seeds |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385412A (en) * | 2008-09-26 | 2009-03-18 | 云南省烟草科学研究所 | Method for reducing germinating time of tobacco coating pilled seeds |
| CN101773004A (en) * | 2010-02-03 | 2010-07-14 | 云南省烟草农业科学研究院 | Method for improving cold resistance of pelleted seed of tobacco |
| CN106576502A (en) * | 2016-12-16 | 2017-04-26 | 玉溪中烟种子有限责任公司 | Negative ion false proofing tobacco coated seed and production method thereof |
| CN108967068A (en) * | 2018-06-25 | 2018-12-11 | 合肥雪立歆农业科技有限公司 | A kind of pepper seed nursery seed method |
-
2020
- 2020-07-06 CN CN202010638531.9A patent/CN111802016A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101385412A (en) * | 2008-09-26 | 2009-03-18 | 云南省烟草科学研究所 | Method for reducing germinating time of tobacco coating pilled seeds |
| CN101773004A (en) * | 2010-02-03 | 2010-07-14 | 云南省烟草农业科学研究院 | Method for improving cold resistance of pelleted seed of tobacco |
| CN106576502A (en) * | 2016-12-16 | 2017-04-26 | 玉溪中烟种子有限责任公司 | Negative ion false proofing tobacco coated seed and production method thereof |
| CN108967068A (en) * | 2018-06-25 | 2018-12-11 | 合肥雪立歆农业科技有限公司 | A kind of pepper seed nursery seed method |
Non-Patent Citations (3)
| Title |
|---|
| 梁文旭 等: "引发激活对烟草种子发芽特性和幼苗抗冷性的效应研究", 《河南农业科学》 * |
| 索文龙 等: "新型材料在烟草种子引发和包衣丸化上的应用", 《种子》 * |
| 鲁黎明 等: "不同消毒剂对烟草种子消毒效果及萌发的影响", 《种子》 * |
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Application publication date: 20201023 |