CN111801415A - 扩增淋巴细胞的方法 - Google Patents

扩增淋巴细胞的方法 Download PDF

Info

Publication number
CN111801415A
CN111801415A CN201880079423.3A CN201880079423A CN111801415A CN 111801415 A CN111801415 A CN 111801415A CN 201880079423 A CN201880079423 A CN 201880079423A CN 111801415 A CN111801415 A CN 111801415A
Authority
CN
China
Prior art keywords
cells
peptide
antigen
lymphocytes
tumor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201880079423.3A
Other languages
English (en)
Chinese (zh)
Inventor
S·波比色
A·哈拉里
G·库克斯
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ludwig Institute for Cancer Research Ltd
Original Assignee
Ludwig Institute for Cancer Research Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ludwig Institute for Cancer Research Ltd filed Critical Ludwig Institute for Cancer Research Ltd
Publication of CN111801415A publication Critical patent/CN111801415A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0635B lymphocytes
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00Cellular immunotherapy
    • A61K40/10Cellular immunotherapy characterised by the cell type used
    • A61K40/11T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00Cellular immunotherapy
    • A61K40/40Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41Vertebrate antigens
    • A61K40/42Cancer antigens
    • A61K40/4201Neoantigens
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0634Cells from the blood or the immune system
    • C12N5/0636T lymphocytes
    • C12N5/0638Cytotoxic T lymphocytes [CTL] or lymphokine activated killer cells [LAK]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/2302Interleukin-2 (IL-2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/2304Interleukin-4 (IL-4)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/2321Interleukin-21 (IL-21)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50Cell markers; Cell surface determinants
    • C12N2501/515CD3, T-cell receptor complex
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/50Cell markers; Cell surface determinants
    • C12N2501/52CD40, CD40-ligand (CD154)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2502/00Coculture with; Conditioned medium produced by
    • C12N2502/11Coculture with; Conditioned medium produced by blood or immune system cells
    • C12N2502/1107B cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
CN201880079423.3A 2017-11-06 2018-11-06 扩增淋巴细胞的方法 Pending CN111801415A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762582163P 2017-11-06 2017-11-06
US62/582,163 2017-11-06
PCT/EP2018/080343 WO2019086711A1 (en) 2017-11-06 2018-11-06 Method for expansion of lymphocytes

Publications (1)

Publication Number Publication Date
CN111801415A true CN111801415A (zh) 2020-10-20

Family

ID=64332272

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201880079423.3A Pending CN111801415A (zh) 2017-11-06 2018-11-06 扩增淋巴细胞的方法

Country Status (7)

Country Link
US (1) US20200338125A1 (https=)
EP (1) EP3707245A1 (https=)
JP (1) JP7357613B2 (https=)
CN (1) CN111801415A (https=)
AU (1) AU2018361561C1 (https=)
CA (1) CA3081479A1 (https=)
WO (1) WO2019086711A1 (https=)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021239083A1 (zh) * 2020-05-29 2021-12-02 上海君赛生物科技有限公司 肿瘤浸润淋巴细胞的种子细胞培养基及其应用
WO2022135525A1 (zh) * 2020-12-24 2022-06-30 苏州沙砾生物科技有限公司 肿瘤浸润淋巴细胞的制备方法及其用途
WO2022143785A1 (en) * 2020-12-31 2022-07-07 Shanghai Cellular Biopharmaceutical Group Ltd. Methods for preparing tumor-infiltrating lymphocytes
WO2022166947A1 (zh) * 2021-02-08 2022-08-11 苏州沙砾生物科技有限公司 肿瘤浸润淋巴细胞的制备方法及其用途
WO2023011433A1 (zh) * 2021-08-03 2023-02-09 苏州沙砾生物科技有限公司 一种修饰的肿瘤浸润淋巴细胞及其用途
WO2023164990A1 (zh) * 2022-03-03 2023-09-07 中山大学孙逸仙纪念医院深汕中心医院 一种自体肿瘤引流淋巴结淋巴细胞的制备方法及其应用
US12110506B2 (en) 2020-11-19 2024-10-08 Suzhou Grit Biotechnology Co., Ltd. Method for culturing tumor infiltrating lymphocytes
WO2025131119A1 (en) * 2023-12-22 2025-06-26 Everest Medicines (China) Co., Ltd. Cancer vaccines and uses thereof

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020095113A1 (en) 2018-11-05 2020-05-14 Ludwig Institute For Cancer Research Ltd Humanized and variant tgf-beta1 specific antibodies and methods and uses thereof
CN113613726B (zh) 2018-11-05 2025-07-15 路德维格癌症研究所有限公司 人源化和变体TGF-β3特异性抗体及其方法和用途
CA3145196A1 (en) * 2019-06-24 2020-12-30 H. Lee Moffitt Cancer Center And Research Institute, Inc. A peptide-based screening method to identify neoantigens for use with tumor infiltrating lymphocytes
JP7337373B2 (ja) * 2019-07-29 2023-09-04 サイアス株式会社 抗原特異的t細胞の製造方法
JP2023509388A (ja) * 2019-12-18 2023-03-08 セービーオ アクティーゼルスカブ 治療用途の腫瘍浸潤性リンパ球を培養するための改善プロセス
US12365871B2 (en) 2020-04-28 2025-07-22 Lyell Immunopharma, Inc. Methods for culturing cells
EP4192941A1 (en) 2020-08-07 2023-06-14 Neogene Therapeutics B.V. Methods to enrich genetically engineered t cells
US20240209313A1 (en) * 2020-08-17 2024-06-27 Northwestern University B-cell based immunotherapy for the treatment of glioblastoma and other cancers
CN112501308B (zh) * 2020-11-30 2025-10-10 中国水产科学研究院珠江水产研究所 一组鳖科动物线粒体全基因组扩增通用引物
WO2022159460A1 (en) * 2021-01-20 2022-07-28 Neogene Therapeutics B.V. Engineered antigen presenting cells
EP4330380A1 (en) * 2021-04-30 2024-03-06 Tigen Pharma SA Single vessel expansion of lymphocytes
US20240207319A1 (en) 2021-05-06 2024-06-27 Ludwig Institute For Cancer Research Ltd Compositions and methods for immunotherapy
WO2022269250A1 (en) * 2021-06-22 2022-12-29 Achilles Therapeutics Uk Limited A method for producing antigen-specific t cells
EP4405464A4 (en) * 2021-09-21 2025-12-03 Univ Chicago METHODS AND COMPOSITION USING PATIENT-DERIVED AUTOLOGOUS NEOANTIGENS FOR CANCER TREATMENT
WO2024261339A1 (en) 2023-06-23 2024-12-26 Tigen Pharma Sa Lymphocyte-stimulatory matrix (lsm) and uses thereof for expansion of lymphocyte populations
WO2025081198A1 (en) * 2023-10-13 2025-04-17 Northwestern University B cell production and expansion

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120100180A1 (en) * 2009-03-05 2012-04-26 Institute Of Microbiology,Chinese Academy Of Sciences Methods for proliferation of antigen-specific t cells
CN106574244A (zh) * 2014-06-11 2017-04-19 保利比奥斯博特有限公司 用细胞因子组合物扩增淋巴细胞用于主动细胞免疫治疗

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6905680B2 (en) 1988-11-23 2005-06-14 Genetics Institute, Inc. Methods of treating HIV infected subjects
US6534055B1 (en) 1988-11-23 2003-03-18 Genetics Institute, Inc. Methods for selectively stimulating proliferation of T cells
US6352694B1 (en) 1994-06-03 2002-03-05 Genetics Institute, Inc. Methods for inducing a population of T cells to proliferate using agents which recognize TCR/CD3 and ligands which stimulate an accessory molecule on the surface of the T cells
US5858358A (en) 1992-04-07 1999-01-12 The United States Of America As Represented By The Secretary Of The Navy Methods for selectively stimulating proliferation of T cells
US7175843B2 (en) 1994-06-03 2007-02-13 Genetics Institute, Llc Methods for selectively stimulating proliferation of T cells
US6692964B1 (en) 1995-05-04 2004-02-17 The United States Of America As Represented By The Secretary Of The Navy Methods for transfecting T cells
US7067318B2 (en) 1995-06-07 2006-06-27 The Regents Of The University Of Michigan Methods for transfecting T cells
US6291430B1 (en) 1997-09-12 2001-09-18 Ludwig Institute For Cancer Research Mage-3 peptides presented by HLA class II molecules
US6867041B2 (en) 2000-02-24 2005-03-15 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
CA2406864A1 (en) 2000-02-24 2001-08-30 Life Technologies Corporation Simultaneous stimulation and concentration of cells
US6797514B2 (en) 2000-02-24 2004-09-28 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
CA2456977C (en) 2001-08-16 2011-06-07 The Trustees Of The University Of Pennsylvania Synthesis and use of reagents for improved dna lipofection and/or slow release prodrug and drug therapies
AU2003265948B8 (en) 2002-09-06 2009-09-03 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Immunotherapy with in vitro-selected antigen-specific lymphocytes after nonmyeloablative lymphodepleting chemotherapy
US9963510B2 (en) 2005-04-15 2018-05-08 Macrogenics, Inc. Covalent diabodies and uses thereof
EP3479844B1 (en) 2005-04-15 2023-11-22 MacroGenics, Inc. Covalent diabodies and uses thereof
EP1995316A1 (de) 2007-05-25 2008-11-26 Qiagen GmbH Verfahren zur schonenden Zellaufreinigung, Zellgewinnung und Transfektion von Zellen
EP2009095A1 (en) 2007-06-28 2008-12-31 Innovalor AG Method of generating glucose-responsive cells
US8629098B2 (en) 2008-01-15 2014-01-14 Yale University Compositions and methods for adoptive and active immunotherapy
US8383099B2 (en) * 2009-08-28 2013-02-26 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Adoptive cell therapy with young T cells
BR112012029066A2 (pt) * 2010-05-14 2020-09-01 The General Hospital Corporation composições e processos de identificação de neoantígenos específicos de tumor.
WO2012044999A2 (en) 2010-10-01 2012-04-05 Ludwig Institute For Cancer Research Ltd. Reversible protein multimers, methods for their production and use
CN104769104A (zh) * 2011-12-12 2015-07-08 细胞药物有限公司 扩大t细胞的方法
CN107002038B (zh) * 2014-09-17 2021-10-15 约翰·霍普金斯大学 用于识别、富集和/或扩增抗原特异性t细胞的试剂和方法
US10973894B2 (en) * 2014-10-02 2021-04-13 The United States Of America, As Represented By The Secretary, Department Of Health And Human Methods of isolating T cells having antigenic specificity for a cancer-specific mutation
WO2016145578A1 (en) * 2015-03-13 2016-09-22 Syz Cell Therapy Co. Methods of cancer treatment using activated t cells
CA3022267A1 (en) * 2016-05-04 2017-11-09 Fred Hutchinson Cancer Research Center Cell-based neoantigen vaccines and uses thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120100180A1 (en) * 2009-03-05 2012-04-26 Institute Of Microbiology,Chinese Academy Of Sciences Methods for proliferation of antigen-specific t cells
CN106574244A (zh) * 2014-06-11 2017-04-19 保利比奥斯博特有限公司 用细胞因子组合物扩增淋巴细胞用于主动细胞免疫治疗

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BOBISSE S等: "Neoantigen-based cancer immunotherapy" *
SHIN CA等: "Co-expression of CD40L with CD70 or OX40L increases B-cell viability and antitumor efficacy" *
沈苏南;徐智;钱晓萍;禹立霞;刘宝瑞;: "CD40L对B淋巴细胞表面分子及分泌IL-12的影响" *

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021239083A1 (zh) * 2020-05-29 2021-12-02 上海君赛生物科技有限公司 肿瘤浸润淋巴细胞的种子细胞培养基及其应用
US12110506B2 (en) 2020-11-19 2024-10-08 Suzhou Grit Biotechnology Co., Ltd. Method for culturing tumor infiltrating lymphocytes
WO2022135525A1 (zh) * 2020-12-24 2022-06-30 苏州沙砾生物科技有限公司 肿瘤浸润淋巴细胞的制备方法及其用途
CN115175988A (zh) * 2020-12-24 2022-10-11 苏州沙砾生物科技有限公司 肿瘤浸润淋巴细胞的培养方法及其用途
CN115175988B (zh) * 2020-12-24 2024-06-14 苏州沙砾生物科技有限公司 肿瘤浸润淋巴细胞的制备方法及其用途
WO2022143785A1 (en) * 2020-12-31 2022-07-07 Shanghai Cellular Biopharmaceutical Group Ltd. Methods for preparing tumor-infiltrating lymphocytes
WO2022166947A1 (zh) * 2021-02-08 2022-08-11 苏州沙砾生物科技有限公司 肿瘤浸润淋巴细胞的制备方法及其用途
CN115315509A (zh) * 2021-02-08 2022-11-08 苏州沙砾生物科技有限公司 肿瘤浸润淋巴细胞的制备方法及其用途
CN115315509B (zh) * 2021-02-08 2024-05-07 苏州沙砾生物科技有限公司 肿瘤浸润淋巴细胞的制备方法及其用途
WO2023011433A1 (zh) * 2021-08-03 2023-02-09 苏州沙砾生物科技有限公司 一种修饰的肿瘤浸润淋巴细胞及其用途
WO2023164990A1 (zh) * 2022-03-03 2023-09-07 中山大学孙逸仙纪念医院深汕中心医院 一种自体肿瘤引流淋巴结淋巴细胞的制备方法及其应用
WO2025131119A1 (en) * 2023-12-22 2025-06-26 Everest Medicines (China) Co., Ltd. Cancer vaccines and uses thereof

Also Published As

Publication number Publication date
US20200338125A1 (en) 2020-10-29
AU2018361561C1 (en) 2025-07-03
JP7357613B2 (ja) 2023-10-06
EP3707245A1 (en) 2020-09-16
CA3081479A1 (en) 2019-05-09
AU2018361561A1 (en) 2020-05-07
JP2021501596A (ja) 2021-01-21
WO2019086711A1 (en) 2019-05-09
AU2018361561B2 (en) 2025-01-30

Similar Documents

Publication Publication Date Title
JP7357613B2 (ja) リンパ球の拡大方法
AU2022201222B2 (en) Delivery of biomolecules to immune cells
US12383601B2 (en) Chimeric antigen receptors and uses thereof
US20210382068A1 (en) Hla single allele lines
US20210324035A1 (en) Composition of ny-eso-1-specific t cell receptors restricted on multiple major histocompatibility complex molecules
US11179450B2 (en) Long chain antigen containing interepitope sequence that promotes antigen presentation to T cells
JP7054397B2 (ja) Mhc-e拘束性エピトープ、結合分子ならびに関連する方法および使用
TW202134430A (zh) 腫瘤細胞疫苗
WO2021247534A2 (en) Compositions and methods for treating merkel cell carcinoma (mcc) using hla class i specific epitopes
US20240327491A1 (en) T cell receptors specific for tumor-associated antigens and methods of use thereof
US20190160098A1 (en) Chimeric antigen receptors and methods of use thereof
US20210113617A1 (en) Methods and compositions for th9 cell mediated cancer treatment
HK40030363A (en) Method for expansion of lymphocytes
WO2022256277A1 (en) Induced pluripotent stem cell-based cancer vaccines
EP4405464A2 (en) Methods and composition using patient-derived autologous neoantigens for treating cancer
CN117460742A (zh) 用于增强的干细胞样记忆t细胞工程化的材料和方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40030363

Country of ref document: HK