CN111748473B - Medical cell culture equipment and cell culture method - Google Patents
Medical cell culture equipment and cell culture method Download PDFInfo
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- CN111748473B CN111748473B CN202010716278.4A CN202010716278A CN111748473B CN 111748473 B CN111748473 B CN 111748473B CN 202010716278 A CN202010716278 A CN 202010716278A CN 111748473 B CN111748473 B CN 111748473B
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- 238000005192 partition Methods 0.000 claims abstract description 17
- 238000004891 communication Methods 0.000 claims abstract description 5
- 239000007788 liquid Substances 0.000 claims description 91
- 238000012360 testing method Methods 0.000 claims description 37
- 230000002093 peripheral effect Effects 0.000 claims description 28
- 238000004659 sterilization and disinfection Methods 0.000 claims description 27
- 235000015097 nutrients Nutrition 0.000 claims description 22
- 238000003756 stirring Methods 0.000 claims description 22
- 238000010438 heat treatment Methods 0.000 claims description 20
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 18
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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- C12M23/00—Constructional details, e.g. recesses, hinges
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Abstract
The invention discloses medical cell culture equipment and a cell culture method, and relates to the technical field of cell culture. The invention comprises an incubator; the top surface of the incubator is hinged with a sealing cover; two operation holes are fixedly arranged on the end surface of the incubator; rubber operating gloves are arranged on the surfaces of the two operating holes; a culture cavity and a transfer cavity are separated in the incubator through a partition plate; a transfer hole is formed at the communication part of the culture cavity and the transfer cavity; a sealing door mechanism is fixedly arranged on the inner wall of the culture cavity; one surface of the sealing door mechanism is in sliding seal with the transfer hole. According to the invention, through the design of the transfer cavity, the sealing door mechanism and the positive pressure air blast component in the incubator, when the device is used for sampling, the probability that external impurity-containing and bacteria-containing air enters the culture dish can be effectively reduced through the cavity separation and positive pressure air blast, and the normal environment during cell culture can be effectively ensured through the reduction of the pollution probability.
Description
Technical Field
The invention belongs to the technical field of cell culture, and particularly relates to medical cell culture equipment and a cell culture method.
Background
The technology of simulating the physiological environment in vivo in vitro, culturing cells taken out of the body and enabling the cells to survive and grow is called cell culture, the research and the application of the cell culture have important significance and are beneficial to explaining life phenomena, pathogenesis and drug screening, generally, the cell culture needs to simulate the physiological environment in vivo, requires sterility, nontoxicity, constant temperature, certain gas environment and buffer environment and proper culture medium, and a cell culture box is generally used for achieving the conditions, so the cell culture box is also an important factor restriction on the development of the cell culture technology. At present, most of common cell culture boxes are constant-temperature carbon dioxide culture boxes, the culture boxes can meet the requirements of cell culture, but have some disadvantages, namely, the first disadvantage is that an external cabin door and an inner layer glass door of the culture box need to be opened when a culture dish is taken and placed, the inside of the culture box is directly exposed in the external environment, the external environment air is directly communicated with the air in the culture box, the pollution in the culture box is easily caused, and a hand (wearing gloves) for taking and placing the culture dish directly extends into the culture box to easily bring in pollutants; drawback two, common cell culture case all has the drawback of observing the interior cell growth condition of culture dish inconvenient on the market, and the culture dish is placed in the culture box, and the culture box door is closed, is not convenient for at any time, observes the interior cell condition of culture dish in real time.
Disclosure of Invention
The invention aims to provide medical cell culture equipment and a cell culture method, which solve the problems that the existing incubator is easy to pollute and is inconvenient to observe cells in a culture dish through the design of a driving mechanism, a monitoring assembly and a culture mechanism.
In order to solve the technical problems, the invention is realized by the following technical scheme:
the invention relates to a medical cell culture device, which comprises an incubator; the top surface of the incubator is hinged with a sealing cover; two operation holes are fixedly arranged on the end face of the incubator; rubber operating gloves are arranged on the surfaces of the two operating holes; a culture cavity and a transfer cavity are separated from the interior of the incubator through a partition plate; a transfer hole is formed at the communication part of the culture cavity and the transfer cavity; a sealing door mechanism is fixedly arranged on the inner wall of the culture cavity; one surface of the sealing door mechanism is in sliding sealing with the transfer hole;
a driving mechanism, an air purifier, a monitoring assembly and two first ultraviolet disinfection lamps are fixedly arranged in the culture cavity respectively; the back of the culture cavity is also fixedly communicated with an air supply pipe; a culture mechanism is fixedly arranged on the inner wall of the culture cavity; two brackets are fixedly connected to the side surface of the incubator and correspond to the positions of the culture mechanisms; a waste liquid storage tank and a nutrient solution supply tank are respectively and fixedly arranged in the two brackets; the surfaces of the waste liquid storage tank and the nutrient solution supply tank are communicated with the culture mechanism through connecting pieces; an observation assembly is fixedly arranged on the surface of the incubator and at a position corresponding to the upper part of the operation hole; one end of the detection port of the observation assembly extends into the culture cavity and is opposite to the driving mechanism;
two second ultraviolet disinfection lamps and positive pressure air blast components are fixedly arranged on the surface of the transfer cavity; a door body is fixedly arranged on the surface of the incubator and corresponds to the position of the transfer cavity; the inside of the transfer cavity is movably provided with a beam frame;
the driving mechanism comprises a fixed seat; the peripheral side surface of the fixed seat is in sliding fit with the culture cavity; the bottom surface of the fixed seat is fixedly connected with a group of damping buffer parts and two symmetrically arranged vibration motors; the bottom ends of the damping buffer parts are fixedly connected with the bottom surface of the incubator; the inner wall of the fixed seat is rotatably connected with a rotating seat through a bearing; the bottom surface of the fixed seat is fixedly connected with a servo motor; one end of the output shaft of the servo motor is fixedly connected with the rotating seat; the surface of the rotating seat is rotationally communicated with a group of limiting assemblies; the inner wall of the fixed seat is fixedly connected with a fixed gear ring; the peripheral side surfaces of one group of limiting assemblies are meshed with the static gear ring; the inner walls of the limiting assemblies are clamped with culture test tubes;
the culture mechanism comprises an inner frame; the back surface of the inner frame is fixedly connected with the culture cavity; a screw rod transmission assembly is fixedly connected between the inner surfaces of the inner frames; the peripheral side surface of the screw rod transmission assembly is connected with a movable seat; the bottom surface of the moving seat is respectively and fixedly provided with a liquid pump and a liquid supply pump; liquid tubes are fixedly communicated with one ends of the liquid pumping port of the liquid pumping pump and the liquid supply port of the liquid supply pump; one end of the liquid outlet of the liquid pump and one end of the liquid suction port of the liquid supply pump are fixedly communicated with corrugated hoses; one end of each of the two corrugated hoses is fixedly connected with the inner frame; one end of the liquid outlet of the liquid pump and one end of the liquid suction port of the liquid supply pump are respectively communicated with the waste liquid storage tank and the nutrient solution supply tank through corrugated hoses; the surface of the moving seat is also fixedly provided with a stirring component;
the limiting assembly comprises a limiting jacket; the peripheral side surface of the culture test tube is clamped with the limiting jacket; the bottom surface of the limiting jacket is rotationally communicated with the rotating seat; the inner wall of the limiting jacket is fixedly provided with a heating sub-cavity; a heating main cavity is fixedly arranged on the inner wall of the rotating seat; one end of the heating sub-cavity is communicated with the heating main cavity; a group of heating pipes is fixedly arranged in the heating main cavity;
the limiting assembly further comprises a shaft rod; the peripheral side surface of the shaft lever is in transmission connection with the rotating seat; the end part of the shaft rod is fixedly connected with the limiting jacket; the peripheral side surface of the shaft lever is fixedly connected with a driven gear; the peripheral side surface of the driven gear is meshed with the static gear ring;
a sealing ring is fixedly arranged at the sliding fit position of the fixed seat and the culture cavity; and a control panel is fixedly arranged on the end face of the incubator.
Preferably, the check valve and the flowmeter are fixedly arranged on the peripheral side surface of the liquid pipe; the circumferential side surface of the gas supply pipe is fixedly provided with a one-way valve; the end part of the air supply pipe is fixedly provided with a connecting joint.
Preferably, the stirring assembly comprises a drive motor; the bottom surface of the driving motor is fixedly connected with the movable seat; one end of the output shaft of the driving motor is fixedly connected with a rotating shaft; a group of stirring blades is fixedly connected to the circumferential side surface of the rotating shaft; the stirring assembly is positioned between the two liquid pipes.
Preferably, the sealing door mechanism comprises a sealing partition door and a group of electric push rods; the surfaces of the electric push rods are fixedly connected with the incubator; the peripheral side surface of the sealing partition door is in sliding fit with the transfer cavity; the movable ends of the electric push rods are fixedly connected with the sealing partition door; the surface of the sealing partition door is matched with the transfer hole; and a sealing gasket strip is fixedly arranged at the sliding fit position of the sealing partition plate and the transfer cavity.
Preferably, the cell culture method of the medical cell culture device comprises the following steps:
SS0001, opening a sealing cover, sequentially placing a culture test tube filled with cells to be cultured and a culture solution on a limiting clamp sleeve in a limiting assembly, closing the sealing cover after the culture test tube is placed, opening a door body at a transfer cavity after the sealing cover is closed, working an air purifier, a first ultraviolet disinfection lamp, a second ultraviolet disinfection lamp and a positive pressure blast assembly, maintaining for a period of time, closing the door body, opening a sealing door mechanism, and entering a formal culture state;
SS002, under the normal culture state, the first ultraviolet disinfection lamp is normally opened, the air purifier works periodically, then culture gas is supplied to the device, and when the device is used, the air supply pipe is communicated with external carbon dioxide supply equipment, under the cooperation of the control panel and the monitoring assembly, then oxygen data, carbon dioxide data and temperature data in the culture cavity and the transfer cavity are maintained to be normal, the vibration motor works normally, and then cells to be cultured are in an oscillation state;
SS003, when a certain culture test tube needs to be subjected to liquid changing work of nutrient solution, a user controls a servo motor to work by setting a control panel, after the servo motor works, the rotary seat is driven to rotate, finally, the test tube to be changed is positioned under a culture mechanism, then, a screw rod transmission assembly works, a stirring assembly and two liquid tubes are positioned in the culture test tube, when the liquid is changed, a liquid pump works for a specified time under the control of the control panel, then, the waste liquid is discharged to a waste liquid storage tank, after the liquid pump works, the liquid supply pump works, after the specified time of working, the nutrient solution in a nutrient solution supply tank is supplemented to the culture test tube, after the nutrient solution is supplemented, the stirring assembly works for a specified time, and after the working, the movable seat returns again under the action of the screw rod transmission assembly;
SS004, when cells in a certain culture test tube need to be observed, a user controls a servo motor to work through setting a control panel, and then drives a rotary seat to rotate after the servo motor works, so that the test tube to be observed is located under an observation assembly;
SS005, when needs shift a certain cultivation test tube, the user shifts this cultivation test tube to the restraint frame that shifts the chamber through rubber operation gloves, after placing, second ultraviolet disinfection lamp and malleation air blast subassembly work, after the work, sealing door mechanism seals shifting the hole, seal the back that finishes, open a body, and when a body was opened, malleation air blast subassembly and second ultraviolet disinfection lamp continued work, shift the back that finishes, close a body, after second ultraviolet disinfection lamp work appointed time, sealing door mechanism reopens.
The invention has the following beneficial effects:
1. according to the invention, through the design of the transfer cavity, the sealing door mechanism and the positive pressure air blast component in the incubator, the probability that external impurity-containing and bacterium-containing air enters the culture dish can be effectively reduced through the modes of separating the cavity and positive pressure air blast when the device is used for sampling, the normal environment during cell culture can be effectively ensured through the reduction of the pollution probability, and the online observation of the culture condition of cells is facilitated through the design of the driving mechanism and the matching design of the observation component.
2. According to the invention, through the design of the vibration motor and the damping buffer piece, the cell sap can uniformly fluctuate up and down in the culture process, so that the shaking state of hands is truly simulated, and the cell sap can be uniformly suspended through maintaining the shaking state.
3. According to the invention, through the design of the culture mechanism, on one hand, the device can complete the waste liquid extraction and new liquid supplement work of the cell culture dish in an automatic assembly line mode, and on the other hand, through the design of the stirring assembly, the mixing speed of cells and new culture liquid can be effectively accelerated.
4. According to the invention, through the design of the main heating cavity, the cell culture temperature can be effectively ensured and maintained, and through the monitoring assembly, the environment in the cell culture process can be monitored on line in real time, so that the continuous cell culture operation is facilitated.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of a medical cell culture apparatus;
FIG. 2 is a schematic view of the structure of FIG. 1 from another angle;
FIG. 3 is a schematic cross-sectional view of FIG. 1;
FIG. 4 is a schematic structural view of a transfer port, a sealing door mechanism, a bundle frame and a positive pressure blower assembly;
FIG. 5 is a schematic view of the driving mechanism;
FIG. 6 is a schematic cross-sectional view of FIG. 5;
FIG. 7 is a schematic structural view of a position limiting assembly;
FIG. 8 is a schematic view of the structure of a culture mechanism;
FIG. 9 is a schematic front view of the structure of FIG. 8;
in the drawings, the components represented by the respective reference numerals are listed below:
1. an incubator; 2. an operation hole; 3. a culture chamber; 4. a transfer chamber; 5. a transfer well; 6. a sealing door mechanism; 7. a drive mechanism; 8. an air purifier; 9. a monitoring component; 10. a first ultraviolet disinfection lamp; 11. a gas supply pipe; 12. a culture mechanism; 13. a bracket; 14. a waste liquid storage tank; 15. a nutrient solution supply tank; 16. an observation component; 17. a second ultraviolet disinfection lamp; 18. a positive pressure blower assembly; 19. a door body; 20. bundling a frame; 21. a fixed seat; 22. a damping buffer; 23. a vibration motor; 24. a rotating base; 25. a servo motor; 26. a limiting component; 27. a stationary ring gear; 28. culturing the test tube; 29. an inner frame; 30. the screw rod transmission assembly; 31. a movable seat; 32. a liquid pump; 33, a liquid supply pump; 34. a liquid pipe; 35. a corrugated hose; 36. a stirring assembly; 37. a limiting jacket; 38. heating the main chamber; 39. a shaft lever; 40. a driven gear; 41. a check valve; 42. a flow meter.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-9, the present invention is a medical cell culture apparatus, comprising an incubator 1; the top surface of the incubator 1 is hinged with a sealing cover; two operation holes 2 are fixedly arranged on the end face of the incubator 1; rubber operating gloves are arranged on the surfaces of the two operating holes 2;
a culture cavity 3 and a transfer cavity 4 are separated from the interior of the incubator 1 through a partition plate; a transfer hole 5 is formed at the communication part of the culture cavity 3 and the transfer cavity 4; a sealing door mechanism 6 is fixedly arranged on the inner wall of the culture cavity 3; one surface of the sealing door mechanism 6 is in sliding sealing with the transfer hole 5;
a driving mechanism 7, an air purifier 8, a monitoring assembly 9 and two first ultraviolet disinfection lamps 10 are respectively and fixedly arranged in the culture cavity 3; the air purifier 8 is used for providing pure air for the device, the air purifier 8 is an existing mechanism and is not repeated herein, the monitoring component 9 comprises a temperature sensor, a carbon dioxide sensor and an oxygen content sensor, and the ultraviolet disinfection lamp 10 is used for ensuring the sterile state in the cavity;
the back of the culture cavity 3 is also fixedly communicated with an air supply pipe 11; the gas supply pipe 11 is connected to an external carbon dioxide supply or discharge device when in use;
a culture mechanism 12 is fixedly arranged on the inner wall of the culture cavity 3; two brackets 13 are fixedly connected to the side surface of the incubator 1 corresponding to the positions of the culture mechanisms 12; a waste liquid storage tank 14 and a nutrient solution supply tank 15 are respectively and fixedly arranged in the two brackets 13; the surfaces of the waste liquid storage tank 14 and the nutrient solution supply tank 15 are communicated with the culture mechanism 12 through connecting pieces; an observation assembly 16 is fixedly arranged on one surface of the incubator 1 and corresponds to the position above the operation hole 2; the observation component 16 is essentially a microscope configured to observe microscopic development of the cells;
one end of the detection port of the observation assembly 16 extends into the culture cavity 3 and is opposite to the driving mechanism 7; two second ultraviolet disinfection lamps 17 and a positive pressure air blast assembly 18 are fixedly arranged on one surface of the transfer cavity 4; the positive pressure air blast component 18 is essentially an air blower which blows air outwards in an internal circulation mode, and the probability that external bacteria-containing air enters the incubator 1 is reduced by blowing air outwards;
a door body 19 is fixedly arranged on the surface of the incubator 1 and corresponds to the position of the transfer cavity 4; the inside of the transfer cavity 4 is movably provided with a bundle frame 20, and the bundle frame 20 is used for limiting a culture test tube 28;
the driving mechanism 7 comprises a fixed seat 21; the peripheral side surface of the fixed seat 21 is in sliding fit with the culture cavity 3; the bottom surface of the fixed seat 21 is fixedly connected with a group of damping buffer parts 22 and two symmetrically arranged vibration motors 23; the bottom ends of the group of damping buffer parts 22 are all fixedly connected with the bottom surface of the incubator 1; the inner wall of the fixed seat 21 is rotatably connected with a rotating seat 24 through a bearing; the bottom surface of the fixed seat 21 is fixedly connected with a servo motor 25; one end of an output shaft of the servo motor 25 is fixedly connected with the rotating seat 24; the surface of the rotating seat 24 is rotatably communicated with a group of limiting assemblies 26; the inner wall of the fixed seat 21 is fixedly connected with a static gear ring 27; the peripheral sides of a group of the limiting assemblies 26 are all meshed with a fixed gear ring 27; due to the meshing connection design of the limiting assemblies 26 and the static gear ring 27, the culture test tubes 28 can rotate while revolving, and the inner walls of one group of limiting assemblies 26 are clamped with the culture test tubes 28;
the culture mechanism 12 comprises an inner frame 29; the back of the inner frame 29 is fixedly connected with the culture cavity 3; a screw rod transmission assembly 30 is fixedly connected between the inner surfaces of the inner frames 29; the peripheral side surface of the screw rod transmission assembly 30 is connected with a movable seat 31; a liquid pump 32 and a liquid supply pump 33 are respectively and fixedly arranged on the bottom surface of the movable seat 31; liquid tubes 34 are fixedly communicated with one ends of the liquid drawing port of the liquid drawing pump 32 and the liquid supply port of the liquid supply pump 33; one end of the liquid outlet of the liquid pump 32 and one end of the liquid suction port of the liquid supply pump 33 are both fixedly communicated with a corrugated hose 35; one end of each of the two corrugated hoses 35 is fixedly connected with the inner frame 29; one end of the liquid outlet of the liquid pump 32 and one end of the liquid suction port of the liquid supply pump 33 are respectively communicated with the waste liquid storage tank 14 and the nutrient solution supply tank 15 through corrugated hoses 35; the surface of the movable seat 31 is also fixedly provided with a stirring component 36.
As further shown in fig. 6 and 5, the restraining assembly 26 includes a restraining collet 37; the side surface of the periphery of the culture test tube 28 is clamped with a limiting jacket 37; the bottom surface of the limiting jacket 37 is in rotary communication with the rotary seat 24; the inner wall of the limiting jacket 37 is fixedly provided with a heating sub-cavity; a heating main cavity 38 is fixedly arranged on the inner wall of the rotating seat 24; one end of the heating sub-cavity is communicated with the heating main cavity 38; a set of heating pipes is fixedly installed inside the heating main chamber 38.
As further shown in fig. 7, the stop assembly 26 further includes a shaft 39; the peripheral side surface of the shaft rod 39 is in transmission connection with the rotating seat 24; the end part of the shaft rod 39 is fixedly connected with the limiting jacket 37; a driven gear 40 is fixedly connected to the peripheral side surface of the shaft lever 39; the peripheral side surface of the driven gear 40 is engaged with the fixed gear ring 27.
As further shown in fig. 8 and 9, a check valve 41 and a flow meter 42 are fixedly mounted on the peripheral side of the liquid pipe 34; a one-way valve is fixedly arranged on the peripheral side surface of the air supply pipe 11; the end of the air supply pipe 11 is fixedly provided with a connecting joint, the check valve 41 is used for preventing liquid from flowing back, and the flow meter 42 is used for counting the liquid extraction amount and the liquid supply total amount.
The stirring assembly 36 includes a drive motor; the bottom surface of the driving motor is fixedly connected with the movable seat 31; one end of the output shaft of the driving motor is fixedly connected with a rotating shaft; a group of stirring blades is fixedly connected to the circumferential side surface of the rotating shaft; the stirring component 36 is positioned between the two liquid tubes 34, the mixing speed of the cells and the culture solution can be enhanced through the design of the stirring component 36, and the stirring component 36 is in a low-speed rotating state during operation.
Further, the sealing door mechanism 6 comprises a sealing partition door and a group of electric push rods; the surfaces of the electric push rods are fixedly connected with the incubator 1; the peripheral side surface of the sealing partition door is in sliding fit with the transfer cavity 4; the movable ends of the electric push rods are fixedly connected with the sealing partition door; the surface of the sealing partition door is matched with the transfer hole 5; and a sealing gasket strip is fixedly arranged at the sliding fit position of the sealing clapboard and the transfer cavity 4.
Further, a sealing ring is fixedly arranged at the sliding fit position of the fixed seat 21 and the culture cavity 3; a control panel is fixedly arranged on the end face of the incubator 1.
Further, a cell culture method of the medical cell culture equipment comprises the following steps:
SS0001, opening a sealing cover, sequentially placing the culture test tube 28 filled with cells to be cultured and culture solution on a limiting jacket 37 in a limiting assembly 26, closing the sealing cover after the culture test tube 28 is placed, opening a door body 19 at a transfer cavity 4 after the sealing cover is closed, operating an air purifier 8, a first ultraviolet disinfection lamp 10, a second ultraviolet disinfection lamp 17 and a positive pressure air blast assembly 18, maintaining for a period of time, closing the door body 19, opening a sealing door mechanism 6, and entering a formal culture state;
SS002, under the normal culture state, the first ultraviolet disinfection lamp 10 is normally open, the air purifier 8 periodically works, then culture gas is supplied to the device, and when the device is used, the air supply pipe 11 is communicated with external carbon dioxide supply equipment, under the cooperation of the control panel and the monitoring assembly 9, then oxygen data, carbon dioxide data and temperature data in the culture cavity 3 and the transfer cavity 4 are maintained to be normal, the vibration motor 23 normally works, and then cells to be cultured are in a vibration state;
SS003, when a certain culture test tube 28 needs to be changed with nutrient solution, the user controls the servo motor 25 to work through setting the control panel, after the servo motor 25 works, then the rotating seat 24 is driven to rotate, finally the test tube to be changed is positioned under the culture mechanism 12, and then, the lead screw drive assembly 30 operates to position the stirring assembly 36 and the two fluid tubes 34 within the culture tube 28, and during fluid change, the liquid pump 32 operates for a predetermined time under the control of the control panel, and then discharges the waste liquid to the waste liquid storage tank 14, and after the operation of the liquid pump 32 is completed, the liquid feed pump 33 operates, after the operation for a predetermined time, the nutrient solution in the nutrient solution supply tank 15 is then replenished to the culture tube 28, after the nutrient solution is supplemented, the stirring assembly 36 works for a designated time, and after the stirring assembly works, the movable seat 31 returns under the action of the screw rod transmission assembly 30;
SS004, when cells in a certain culture test tube 28 need to be observed, a user controls the servo motor 25 to work through setting the control panel, and then the servo motor 25 drives the rotary seat 24 to rotate after working, so that the test tube to be observed is finally positioned under the observation assembly 16;
SS005, when needing to shift a certain cultivation test tube 28, the user shifts this cultivation test tube 28 to the bundle frame 20 that shifts the chamber 4 through rubber operation gloves, after the placement, second ultraviolet disinfection lamp 17 and the work of malleation blast air subassembly 18, after the work, sealing door mechanism 6 seals shifting hole 5, after closing, open door body 19, and when door body 19 opened, malleation blast air subassembly 18 and second ultraviolet disinfection lamp 17 continued work, after shifting, close door body 19, after second ultraviolet disinfection lamp 17 work appointed time, sealing door mechanism 6 reopens.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.
Claims (5)
1. A medical cell culture apparatus comprising an incubator (1); the top surface of the incubator (1) is hinged with a sealing cover; two operation holes (2) are fixedly arranged on the end face of the incubator (1); rubber operation gloves, its characterized in that are all installed on two handle hole (2) surfaces:
a culture cavity (3) and a transfer cavity (4) are separated from the interior of the incubator (1) through a partition plate; a transfer hole (5) is formed at the communication part of the culture cavity (3) and the transfer cavity (4); a sealing door mechanism (6) is fixedly arranged on the inner wall of the culture cavity (3); one surface of the sealing door mechanism (6) is in sliding seal with the transfer hole (5);
a driving mechanism (7), an air purifier (8), a monitoring assembly (9) and two first ultraviolet disinfection lamps (10) are respectively and fixedly arranged in the culture cavity (3); the back of the culture cavity (3) is also fixedly communicated with an air supply pipe (11); a culture mechanism (12) is fixedly arranged on the inner wall of the culture cavity (3); two brackets (13) are fixedly connected to the side surface of the incubator (1) and correspond to the positions of the culture mechanisms (12); a waste liquid storage tank (14) and a nutrient solution supply tank (15) are respectively and fixedly arranged in the two brackets (13); the surfaces of the waste liquid storage tank (14) and the nutrient solution supply tank (15) are communicated with the culture mechanism (12) through connecting pieces; an observation assembly (16) is fixedly arranged on one surface of the incubator (1) and corresponds to the position above the operation hole (2); one end of the detection port of the observation assembly (16) extends into the culture cavity (3) and is opposite to the driving mechanism (7);
two second ultraviolet disinfection lamps (17) and a positive pressure air blast assembly (18) are fixedly arranged on one surface of the transfer cavity (4); a door body (19) is fixedly arranged on the surface of the incubator (1) and corresponds to the position of the transfer cavity (4); a bundling frame (20) is movably arranged in the transfer cavity (4);
the driving mechanism (7) comprises a fixed seat (21); the peripheral side surface of the fixed seat (21) is in sliding fit with the culture cavity (3); the bottom surface of the fixed seat (21) is fixedly connected with a group of damping buffer parts (22) and two symmetrically arranged vibration motors (23); the bottom ends of the damping buffer parts (22) are fixedly connected with the bottom surface of the incubator (1); the inner wall of the fixed seat (21) is rotatably connected with a rotating seat (24) through a bearing; the bottom surface of the fixed seat (21) is fixedly connected with a servo motor (25); one end of an output shaft of the servo motor (25) is fixedly connected with the rotating seat (24); the surface of the rotating seat (24) is rotationally communicated with a group of limiting assemblies (26); the inner wall of the fixed seat (21) is fixedly connected with a static gear ring (27); the peripheral side surfaces of a group of limiting assemblies (26) are all meshed with a static gear ring (27); the inner walls of the limiting assemblies (26) are clamped with culture test tubes (28);
the culture mechanism (12) comprises an inner frame (29); the back of the inner frame (29) is fixedly connected with the culture cavity (3); a screw rod transmission assembly (30) is fixedly connected between the inner surfaces of the inner frames (29); the peripheral side surface of the screw rod transmission assembly (30) is connected with a movable seat (31); a liquid pump (32) and a liquid supply pump (33) are respectively and fixedly arranged on the bottom surface of the movable seat (31); liquid tubes (34) are fixedly communicated with a liquid drawing port of the liquid drawing pump (32) and one end of a liquid supply port of the liquid supply pump (33); one end of the liquid outlet of the liquid pump (32) and one end of the liquid suction port of the liquid supply pump (33) are fixedly communicated with a corrugated hose (35); one ends of the two corrugated hoses (35) are fixedly connected with the inner frame (29); one end of a liquid outlet of the liquid pump (32) and one end of a liquid suction port of the liquid supply pump (33) are respectively communicated with the waste liquid storage tank (14) and the nutrient solution supply tank (15) through corrugated hoses (35); the surface of the moving seat (31) is also fixedly provided with a stirring component (36);
the limiting assembly (26) comprises a limiting jacket (37); the peripheral side surface of the culture test tube (28) is clamped with a limiting jacket (37); the bottom surface of the limiting jacket (37) is rotationally communicated with the rotating seat (24); the inner wall of the limiting jacket (37) is fixedly provided with a heating sub-cavity; a heating main cavity (38) is fixedly arranged on the inner wall of the rotating seat (24); one end of the heating sub-cavity is communicated with the heating main cavity (38); a group of heating pipes are fixedly arranged in the heating main cavity (38);
the stop assembly (26) further comprises a shaft (39); the peripheral side surface of the shaft lever (39) is in transmission connection with the rotating seat (24); the end part of the shaft lever (39) is fixedly connected with the limiting jacket (37); a driven gear (40) is fixedly connected to the peripheral side surface of the shaft lever (39); the peripheral side surface of the driven gear (40) is meshed with the static gear ring (27);
a sealing ring is fixedly arranged at the sliding fit position of the fixed seat (21) and the culture cavity (3); a control panel is fixedly arranged on the end face of the incubator (1).
2. The medical cell culture apparatus according to claim 1, wherein a check valve (41) and a flow meter (42) are fixedly installed on the peripheral side surfaces of the two liquid tubes (34); a one-way valve is fixedly arranged on the peripheral side surface of the air supply pipe (11); the end part of the air supply pipe (11) is fixedly provided with a connecting joint.
3. The medical cell culture apparatus of claim 1, wherein the agitation assembly (36) comprises a drive motor; the bottom surface of the driving motor is fixedly connected with the movable seat (31); one end of the output shaft of the driving motor is fixedly connected with a rotating shaft; a group of stirring blades is fixedly connected to the circumferential side surface of the rotating shaft; the stirring assembly (36) is located between the two liquid pipes (34).
4. The medical cell culture apparatus according to claim 1, wherein the sealing door mechanism (6) comprises a sealing partition door and a set of electric push rods; the surfaces of the electric push rods are fixedly connected with the incubator (1); the peripheral side surface of the sealing partition door is in sliding fit with the transfer cavity (4); the movable ends of the electric push rods are fixedly connected with the sealing partition door; the surface of the sealing partition door is matched with the transfer hole (5); and a sealing gasket strip is fixedly arranged at the sliding fit position of the sealing clapboard and the transfer cavity (4).
5. The cell culture method of the medical cell culture equipment according to any one of claims 1 to 4, comprising the steps of:
SS0001, opening a sealing cover, sequentially placing a culture test tube (28) filled with cells to be cultured and a culture solution on a limiting jacket (37) in a limiting assembly (26), closing the sealing cover after the culture test tube (28) is placed in, opening a door body (19) at a transfer cavity (4) after the sealing cover is closed, operating an air purifier (8), a first ultraviolet disinfection lamp (10), a second ultraviolet disinfection lamp (17) and a positive pressure blast assembly (18), maintaining for a period of time, closing the door body (19), and opening a sealing door mechanism (6) to enter a formal culture state;
SS002, under the normal culture state, the first ultraviolet disinfection lamp (10) is normally opened, the air purifier (8) works periodically, then culture gas is supplied to the device, in addition, when the device is used, the air supply pipe (11) is communicated with external carbon dioxide supply equipment, under the matching of the control panel and the monitoring assembly (9), then oxygen data, carbon dioxide data and temperature data in the culture cavity (3) and the transfer cavity (4) are maintained to be normal, the vibration motor (23) works normally, and then cells to be cultured are in the oscillation state;
SS003, when a certain culture test tube (28) needs to be subjected to liquid changing work of nutrient solution, a user controls a servo motor (25) to work through setting a control panel, after the servo motor (25) works, the rotary seat (24) is driven to rotate, finally, the test tube to be changed is positioned under a culture mechanism (12), then, a screw rod transmission assembly (30) works, then, a stirring assembly (36) and two liquid tubes (34) are positioned inside the culture test tube (28), when the liquid is changed, a liquid pump (32) works for a specified time under the control of the control panel, then, waste liquid is discharged to a waste liquid storage tank (14), after the liquid pump (32) works, a liquid supply pump (33) works, after the working for the specified time, the nutrient solution in a nutrient solution supply tank (15) is supplemented to the culture test tube (28), after the nutrient solution is supplemented, the stirring assembly (36) works for the specified time, after the work is finished, the movable seat (31) returns under the action of the screw rod transmission assembly (30);
SS004, when cells in a certain culture test tube (28) need to be observed, a user controls a servo motor (25) to work through setting a control panel, and then the servo motor (25) drives a rotating seat (24) to rotate after working, so that the test tube to be observed is located right below an observation assembly (16);
SS005, when needing to shift a certain cultivation test tube (28), the user shifts this cultivation test tube (28) to restrainting frame (20) of shifting chamber (4) through rubber operation gloves on, after placing, second ultraviolet disinfection lamp (17) and malleation air blast subassembly (18) work, after the work, sealing door mechanism (6) are sealed shifting hole (5), after closing, open door body (19), and when door body (19) were opened, malleation air blast subassembly (18) and second ultraviolet disinfection lamp (17) continue to work, after the transfer finishes, close door body (19), after second ultraviolet disinfection lamp (17) work appointed time, sealing door mechanism (6) reopen.
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| CN112430543B (en) * | 2020-12-18 | 2024-04-09 | 河南茵特赛尔生物技术有限公司 | Automatic detection and supplement device for cell culture solution |
| CN113214970B (en) * | 2021-05-07 | 2022-05-17 | 锦州医科大学 | Self-adaptive adjustment somatic cell culture device and culture method thereof |
| CN113439587A (en) * | 2021-08-18 | 2021-09-28 | 宁波仟想科技有限公司 | Intelligent cultivation device for agricultural product planting based on Internet of things |
| CN113879682A (en) * | 2021-09-18 | 2022-01-04 | 南京帝基生物科技有限公司 | Kit for genetic colorectal cancer gene detection, and preparation method and system thereof |
| CN114686369B (en) * | 2022-06-02 | 2022-08-26 | 青岛大学附属医院 | Live bacteria subculture detection equipment for preventing leakage infection |
| CN115322899B (en) * | 2022-06-07 | 2024-05-24 | 济宁医学院 | Cell culture device for cell biology experiments |
| CN117586855A (en) * | 2023-12-04 | 2024-02-23 | 苏强 | Incubator for laboratory |
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| CN104169413B (en) * | 2012-02-29 | 2016-01-20 | 株式会社Jtec | There is the cell culture apparatus that nutrient solution changes function |
| CN204342804U (en) * | 2014-12-18 | 2015-05-20 | 宁波新芝生物科技股份有限公司 | A kind of cell cultures all-in-one |
| JP3201607U (en) * | 2015-10-06 | 2015-12-17 | バイオメディカ・ソリューション株式会社 | Cell culture system |
| EP3366758A4 (en) * | 2015-10-20 | 2019-05-08 | Rohto Pharmaceutical Co., Ltd. | Cell treatment device |
| CN109554287A (en) * | 2018-12-21 | 2019-04-02 | 华中科技大学同济医学院附属协和医院 | A kind of medical experiment culture apparatus |
| CN111205983A (en) * | 2020-03-16 | 2020-05-29 | 京诺(上海)细胞生物技术有限公司 | Integrated adherent cell culture separation device |
| CN111334429A (en) * | 2020-03-25 | 2020-06-26 | 北京双诚联盈净化工程技术有限公司 | Modular multifunctional cell workstation |
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