CN214991653U - External embryo culture device is used to ox - Google Patents
External embryo culture device is used to ox Download PDFInfo
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- CN214991653U CN214991653U CN202122688148.7U CN202122688148U CN214991653U CN 214991653 U CN214991653 U CN 214991653U CN 202122688148 U CN202122688148 U CN 202122688148U CN 214991653 U CN214991653 U CN 214991653U
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- shell
- pipe
- fixedly arranged
- fixed mounting
- rotary seat
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- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 30
- 238000007789 sealing Methods 0.000 claims abstract description 20
- 238000012544 monitoring process Methods 0.000 claims abstract description 14
- 241000283690 Bos taurus Species 0.000 claims abstract description 12
- 230000005540 biological transmission Effects 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims description 30
- 238000012360 testing method Methods 0.000 claims description 16
- 238000000338 in vitro Methods 0.000 claims description 13
- 230000002093 peripheral effect Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 abstract description 13
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 238000012258 culturing Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 210000002257 embryonic structure Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 230000035939 shock Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 239000002912 waste gas Substances 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- JCXJVPUVTGWSNB-UHFFFAOYSA-N nitrogen dioxide Inorganic materials O=[N]=O JCXJVPUVTGWSNB-UHFFFAOYSA-N 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
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Abstract
The utility model provides an external embryo culture device for cattle, which relates to the technical field of embryo culture devices and comprises a bracket, a shell is fixedly arranged on the top surface of the bracket, a group of micro-vibration motors are fixedly arranged on the bottom surface of the shell, two symmetrically arranged operation components are arranged on the surface of the shell, a sealing cover is arranged on the top surface of the shell, an air inlet pipe and an exhaust pipe are respectively and fixedly arranged on the side surface of the shell, a monitoring component is fixedly arranged on the other side surface of the shell, a liquid inlet pipe and a servo motor are respectively and fixedly arranged on the bottom of the shell, a rotary seat is rotationally connected with the inner wall of the shell, the bottom end of the rotary seat is rotationally communicated with the liquid inlet pipe, an output shaft of the servo motor is in transmission connection with the rotary seat, a temperature bath main cavity communicated with the liquid inlet pipe is fixedly arranged inside the rotary seat, a group of reciprocating push rods is fixedly arranged on the bottom surface of the rotary seat, be used for solving the relatively poor and inconvenient problem of carrying out the operation to the cultivateing body of current culture apparatus to the analog degree of parent.
Description
Technical Field
The utility model belongs to the technical field of embryo culture device technique and specifically relates to an external embryo culture device is used to ox.
Background
The embryo in vitro culture technology is one of the most basic key technologies for embryo in vitro production in embryo engineering, namely, the embryo is placed in a special container and is continuously developed in vitro, the requirements on culture solution, sterility and other conditions are strict, the traditional embryo in vitro culture device is mainly a culture dish or a culture disc, the culture device can only provide a static, macroscopic and two-dimensional in vitro growth environment for the embryo and is greatly different from the in vivo growth environment of the embryo, in order to solve the problem, the patent document with the publication number of CN210340932U discloses an in vitro culture device for the bovine embryo, although the technical scheme solves the problems in the background technology to a certain extent, the simulation condition of the embryo to a parent in actual work still needs to be improved, and the technical scheme is not convenient for operating the culture body in culture operation, based on this, the utility model provides an external embryo culture device is used to ox to solve the problem that proposes among the above-mentioned background art.
SUMMERY OF THE UTILITY MODEL
The utility model provides an external embryo culture device is used to ox to solve the relatively poor and not convenient for of the current culture apparatus who provides among the above-mentioned background art problem of operating the operation to the cultivateing body of the analog degree of parent.
The technical scheme of the utility model is realized like this:
an external embryo culture device for cattle comprises a bracket, wherein a shell is fixedly arranged on the top surface of the bracket, a group of micro-vibration motors is fixedly arranged on the bottom surface of the shell, two symmetrically-arranged operation assemblies are arranged on the surface of the shell, a sealing cover is arranged on the top surface of the shell, an air inlet pipe and an exhaust pipe are respectively and fixedly arranged on the side surface of the shell, a monitoring assembly is fixedly arranged on the other side surface of the shell, a liquid inlet pipe and a servo motor are respectively and fixedly arranged on the bottom of the shell, a rotary seat is rotatably connected to the inner wall of the shell, the bottom end of the rotary seat is rotatably communicated with the liquid inlet pipe, the output shaft of the servo motor is in transmission connection with the rotary seat, a main warm bath cavity communicated with the liquid inlet pipe is fixedly arranged inside the rotary seat, a group of reciprocating push rods is fixedly arranged on the bottom surface of the rotary seat, and a driving frame is fixedly arranged at the movable end of the reciprocating push rods, the inner wall of the rotary seat is provided with a plurality of groups of material bearing modules distributed in a linear array, the side surfaces of the material bearing modules are in transmission connection with the driving frame, and the two ends of each material bearing module are in rotation connection with the rotary seat.
Preferably, two the operating assembly all includes the control tube, the week side and the casing fixed connection of control tube, the tail end fixed mounting of control tube has the rubber gloves.
Preferably, the surface of the driving frame and the position corresponding to each group of material bearing modules are fixedly provided with a transmission toothed plate, and the circumferential side surfaces of the air inlet pipe and the air outlet pipe are fixedly provided with valves.
Preferably, hold the material module including holding the material pipe, the inside of holding the material pipe has been seted up warm bath ring chamber and has been placed the cavity by outer to interior in proper order, the inner wall joint of placing the cavity has the cultivation test tube, the all side fixed mounting of holding the material pipe has the branch pipe that two symmetries set up, two the one end of branch pipe all with the fixed intercommunication in warm bath ring chamber, two the other end of branch pipe all rotates the intercommunication with the warm bath main cavity, one the all side fixed mounting of branch pipe has driven gear, driven gear's all side and the meshing of the transmission pinion rack that corresponds the position.
Preferably, the top end of the placing cavity is open, the shape of the placing cavity is matched with that of the culture test tube, and the axis of the branch tube is perpendicular to that of the material bearing tube.
Preferably, the fixed sealed rubber ring that is provided with in sealed lid and the junction of casing, the fixed observation window that is provided with of inner wall of sealed lid, the terminal surface fixed mounting of casing has control panel, the one end and the control panel electricity of monitoring subassembly are connected.
By adopting the technical scheme, the beneficial effects of the utility model are that:
the utility model can make the device highly absorb the culture operation of the external embryo of the cattle, and the device can make the culture test tube synchronously swing, rotate and micro-vibrate when culturing the operation, and can truly simulate the parent condition when culturing by the synchronous occurrence of the above motion, thereby effectively promoting the development of the embryo by improving the simulation degree, on one hand, the sterile environment in the shell can be effectively ensured by the design of the operation component and the sealing cover, on the other hand, the culture test tube can be rapidly operated when culturing the operation, thereby effectively improving the use flexibility and the practicability of the device by the realization of the above functions, and the constant temperature keeping effect when culturing the embryo can be effectively improved by the design of the warm bath main cavity and the warm bath annular cavity, thereby being beneficial to improving the survival rate of the embryo.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without inventive exercise.
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic top view of the structure of fig. 1 according to the present invention;
fig. 3 is a cross-sectional view of the present invention;
FIG. 4 is a schematic view of the structure of the rotary seat and the culture tube of the present invention;
fig. 5 is a schematic structural view of the reciprocating push rod, the driving rack and the driving toothed plate of the present invention;
FIG. 6 is a schematic structural view of the material carrying pipe and the driven gear of the present invention;
fig. 7 is a schematic view of the sectional structure of the material-holding module of the present invention.
Wherein:
1-a scaffold; 2-a shell; 3-a micro-vibration motor; 4-operating the components; 5-sealing cover; 6, an air inlet pipe; 7-an exhaust pipe; 8-a monitoring component; 9-a liquid inlet pipe; 10-a servo motor; 11-a rotary seat; 12-a main warm bath cavity; 13-reciprocating push rod; 14-a drive frame; 15-a material bearing module; 16-a driving toothed plate; 17-a material bearing pipe; 18-warm bath ring cavity; 19-placing the cavity; 20-culture test tubes; 21-branch pipe; 22-a driven gear; 23-observation window.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Examples
Referring to fig. 1-7, an in vitro embryo culture device for cattle comprises a bracket 1, a casing 2 is fixedly installed on the top surface of the bracket 1, a group of shock absorption pad feet are installed on the bottom surface of the bracket 1, and the shock absorption pad feet are arranged to absorb shock for the casing 2 when a micro-vibration motor 3 works;
a group of micro-vibration motors 3 are fixedly arranged on the bottom surface of the shell 2, when the micro-vibration motors 3 work, the vibration frequency is output in a set state, and the vibration output of the micro-vibration motors 3 is utilized, so that the vibration environment during embryo culture is simulated;
the surface of the shell 2 is provided with two symmetrically arranged operation components 4, each of the two operation components 4 comprises an operation tube, the operation tube is of a hollow tubular structure with two open ends, the peripheral side surface of the operation tube is fixedly connected with the shell 2, and the tail end of the operation tube is fixedly provided with a rubber glove;
the top surface of the shell 2 is provided with a sealing cover 5, the sealing cover 5 is hinged with the shell 2, the top surface of the shell 2 is fixedly provided with a locking piece matched with the sealing cover 5, the surface of the sealing cover 5 is fixedly provided with a fastener matched with the locking piece, the joint of the sealing cover 5 and the shell 2 is fixedly provided with a sealing rubber ring, the sealing rubber ring is arranged to ensure the sealing property of the joint of the sealing cover 5 and the shell 2, the inner wall of the sealing cover 5 is fixedly provided with an observation window 23, the end surface of the shell 2 is fixedly provided with a control panel, the surface of the control panel is respectively provided with a display screen and a central control key, the display screen is arranged to display monitoring data of the monitoring assembly 8 and the working state of the culture device, a singlechip is fixedly arranged in the control panel, and the model of the singlechip is STM8S003F3P 6;
an air inlet pipe 6 and an air outlet pipe 7 are fixedly installed on the side surface of the shell 2 respectively, the air inlet pipe 6 is used for filling oxygen, nitrogen and carbon dioxide for culture into the shell 2, the air outlet pipe 7 is used for discharging waste gas of the device in the culture process, the air inlet state of the air inlet pipe 6 and the air outlet state of the air outlet pipe 7 are controlled by a control panel, and valves are fixedly installed on the peripheral side surfaces of the air inlet pipe 6 and the air outlet pipe 7;
the other side surface of the shell 2 is fixedly provided with a monitoring assembly 8, and the monitoring assembly 8 comprises an oxygen sensor for monitoring oxygen concentration, a carbon dioxide sensor for monitoring carbon dioxide concentration and a temperature and humidity sensor for monitoring temperature and humidity data in the shell 2;
one end of the monitoring component 8 is electrically connected with the control panel, the bottom of the shell 2 is respectively and fixedly provided with a liquid inlet pipe 9 and a servo motor 10, when in use, the liquid inlet pipe 9 is communicated with external warm water feeding equipment, the inner wall of the shell 2 is rotationally connected with a rotary seat 11, the bottom end of the rotary seat 11 is rotationally communicated with the liquid inlet pipe 9, an output shaft of the servo motor 10 is in transmission connection with the rotary seat 11, the interior of the rotary seat 11 is fixedly provided with a warm bath main cavity 12 communicated with the liquid inlet pipe 9, the bottom surface of the rotary seat 11 is fixedly provided with a group of reciprocating push rods 13, the movable end of the group of reciprocating push rods 13 is fixedly provided with a driving frame 14, the inner wall of the rotary seat 11 is provided with a plurality of groups of material bearing modules 15 distributed in a linear array manner, the side surface of each material bearing module 15 is in transmission connection with the driving frame 14, the surface of the driving frame 14 and the position corresponding to each group of material bearing modules 15 are respectively and fixedly provided with a driving toothed plate 16, the two ends of each material bearing module 15 are rotationally connected with the rotary seat 11, in this embodiment, the branch pipe 21 in the material receiving module 15 is connected with the screw base 11 in a rotating manner.
The material bearing module 15 comprises a material bearing pipe 17, a warm bath annular cavity 18 and a placing cavity 19 are sequentially formed in the material bearing pipe 17 from outside to inside, a culture test tube 20 is clamped on the inner wall of the placing cavity 19, and during culture operation, culture solution and external bovine embryos are supplemented into the culture test tube 20;
the top end opening of the placing cavity 19 is matched with the shape of the placing cavity 19 and the shape of the culture test tube 20, two symmetrically arranged branch pipes 21 are fixedly mounted on the peripheral side face of the material bearing pipe 17, the axis of each branch pipe 21 is perpendicular to the axis of the material bearing pipe 17, one end of each branch pipe 21 is fixedly connected with the material bearing pipe 17, the other end of each branch pipe 21 is rotatably connected with the rotary seat 11, one end of each branch pipe 21 is communicated with the warm bath annular cavity 18, the other end of each branch pipe is communicated with the warm bath main cavity 12, it is to be noted that a sealing rubber ring is arranged at the rotary connection position of each branch pipe 21 and the rotary seat 11, water leakage at the rotary connection position of the branch pipe 21 and the rotary seat 11 is avoided, a sealing effect is achieved, driven gears 22 are fixedly mounted on the peripheral side face of each branch pipe 21, and the peripheral side face of each driven gear 22 is meshed with the transmission toothed plates 16 at the corresponding position.
The working principle is as follows: the device is mainly suitable for the culture operation of in vitro embryos for cattle, before the device is used, a sealing cover 5 is opened, test tubes 20 to be cultured with embryos and culture solution are regularly placed in various material bearing pipes 17, vessels and liquid supplementing devices for storing redundant culture solution are placed in the shell 2 or on a rotary seat 11 in advance, the interior of the shell 2 is pre-sterilized before the culture test tubes 20 and related devices are placed, after the culture test tubes 20 and the related devices are placed, secondary sterilization is performed in the shell 2, during the culture operation, a liquid inlet pipe 9 fills constant-temperature water into a temperature bath main cavity 12 and a temperature bath annular cavity 18, the temperature during the culture is further built, an air inlet pipe 6 and an air outlet pipe 7 work at proper time, then culture gas is filled into the shell 2, waste gas generated during the culture process is discharged at proper time, and during the culture operation, a micro-vibration motor 3, a servo motor 10 and a reciprocating push rod 13 work synchronously and periodically, after the micro-vibration motor 3 works, the culture test tube 20 is driven to generate micro-vibration, the servo motor 10 works, the rotary seat 11 is driven to rotate, after the reciprocating push rod 13 works, the culture test tube 20 is driven to reciprocate within +/-30 degrees, the culture environment of the parent body under the real condition is simulated through the generation of micro-vibration, rotation and swinging states, and when the culture test tube 20 needs to be subjected to related operation, the operation assembly 4 can be used for performing rapid operation.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (6)
1. An in vitro embryo culture device for cattle, which comprises a bracket (1), wherein the top surface of the bracket (1) is fixedly provided with a shell (2), and is characterized in that,
the bottom surface fixed mounting of casing (2) has a set of microvibration motor (3), the surface mounting of casing (2) has operation subassembly (4) that two symmetries set up, sealed lid (5) are installed to the top surface of casing (2), the side of casing (2) is fixed mounting respectively has intake pipe (6) and blast pipe (7), the another side fixed mounting of casing (2) has monitoring subassembly (8), the bottom of casing (2) is fixed mounting respectively has feed liquor pipe (9) and servo motor (10), the inner wall of casing (2) rotates and is connected with revolves seat (11), the bottom and the feed liquor pipe (9) of revolving seat (11) rotate the intercommunication, the output shaft and the revolving seat (11) transmission of servo motor (10) are connected, the inside fixed of revolving seat (11) is seted up the warm bath main cavity (12) that communicate with feed liquor pipe (9), revolve the bottom surface fixed mounting of seat (11) have a set of reciprocal push rod (13), a set of the expansion end fixed mounting of reciprocal push rod (13) has drive frame (14), the inner wall of revolving seat (11) is installed a plurality of groups and is linear array distribution hold material module (15), every hold the side of material module (15) all to be connected with drive frame (14) transmission, every hold the both ends of material module (15) all to rotate with revolving seat (11) and be connected.
2. The in vitro embryo culture device for cattle according to claim 1, wherein the two operation components (4) comprise operation tubes, the peripheral sides of the operation tubes are fixedly connected with the shell (2), and rubber gloves are fixedly arranged at the tail ends of the operation tubes.
3. The in vitro embryo culture device for cattle according to claim 1, wherein the surface of the driving frame (14) and the position corresponding to each group of material bearing modules (15) are fixedly provided with transmission toothed plates (16), and the circumferential side surfaces of the air inlet pipe (6) and the air outlet pipe (7) are fixedly provided with valves.
4. The external embryo culture device is used to ox according to claim 1, characterized in that, hold material module (15) including holding pipe (17), the inside of holding pipe (17) has seted up warm bath ring chamber (18) and has placed cavity (19) from outer to interior in proper order, the inner wall joint of placing cavity (19) has cultivateed test tube (20), the all side fixed mounting that holds pipe (17) has two branch pipes (21) that the symmetry set up, two the one end of branch pipe (21) all with warm bath ring chamber (18) fixed intercommunication, two the other end of branch pipe (21) all rotates the intercommunication with warm bath main cavity (12), one the all side fixed mounting of branch pipe (21) has driven gear (22), the all side of driven gear (22) meshes with the transmission pinion rack (16) that correspond the position.
5. An in vitro embryo culture device for cattle according to claim 4, wherein the top end of the placing cavity (19) is open and the shape of the placing cavity (19) is adapted to the shape of the culture test tube (20), and the axis of the branch tube (21) is perpendicular to the axis of the material bearing tube (17).
6. The in vitro embryo culture device for cattle according to claim 1, wherein a sealing rubber ring is fixedly arranged at the joint of the sealing cover (5) and the shell (2), an observation window (23) is fixedly arranged on the inner wall of the sealing cover (5), a control panel is fixedly arranged on the end surface of the shell (2), and one end of the monitoring component (8) is electrically connected with the control panel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122688148.7U CN214991653U (en) | 2021-11-05 | 2021-11-05 | External embryo culture device is used to ox |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122688148.7U CN214991653U (en) | 2021-11-05 | 2021-11-05 | External embryo culture device is used to ox |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214991653U true CN214991653U (en) | 2021-12-03 |
Family
ID=79123777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122688148.7U Expired - Fee Related CN214991653U (en) | 2021-11-05 | 2021-11-05 | External embryo culture device is used to ox |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214991653U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117586855A (en) * | 2023-12-04 | 2024-02-23 | 苏强 | Incubator for laboratory |
-
2021
- 2021-11-05 CN CN202122688148.7U patent/CN214991653U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117586855A (en) * | 2023-12-04 | 2024-02-23 | 苏强 | Incubator for laboratory |
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211203 |