CN111733073B

CN111733073B - Cell crusher for cosmetic preparation and crushing process method

Info

Publication number: CN111733073B
Application number: CN202010593623.XA
Authority: CN
Inventors: 肖海兵
Original assignee: Dongying Fengqi Biotechnology Development Co ltd
Current assignee: DONGYING FENGQI BIOTECHNOLOGY DEVELOPMENT Co.,Ltd.
Priority date: 2020-06-27
Filing date: 2020-06-27
Publication date: 2021-05-07
Anticipated expiration: 2040-06-27
Also published as: CN111733073A

Abstract

The invention relates to the technical field of cosmetic preparation, in particular to an ultrasonic cell disruption instrument special for cosmetic preparation and a cell disruption process method. By adopting the method of the cell disruption instrument, the cooling tank I and the cooling tank II generate reverse rotation, so that the rapid cooling of the interior of the cooling tank II is ensured, the cells are prevented from being killed and killed by high temperature, the activity of the cells is ensured, and the cell disruption instrument can be effectively used for preparing cosmetics.

Description

Cell crusher for cosmetic preparation and crushing process method

Technical Field

The invention relates to the technical field of cosmetic preparation, in particular to an ultrasonic cell disruption instrument and a cell disruption process method used in a cosmetic preparation process.

Background

In the preparation of cosmetics, it is generally necessary to crush cells of animal and plant materials to extract nutrients such as concentrated amino acids and vitamins.

The cell disruption method can be mainly divided into: mechanical and non-mechanical crushing. Among them, the ultrasonic crushing method in mechanical crushing is more commonly used. The ultrasonic cell crusher is also named as an ultrasonic microwave synergistic extractor, an ultrasonic cell cracking instrument and an ultrasonic nano material crusher. Generally comprises an ultrasonic generator and a transducer. Ultrasonic waves are elastic mechanical waves, both in a wave form and in an energy form, energy passes through a liquid medium to become dense small bubbles, the small bubbles burst rapidly, molecular vibration caused by the ultrasonic waves is prevented, and partial energy is converted into local high heat (42-43 ℃), because the critical lethal temperature of normal tissues is 45.7 ℃, the metabolism of cells is obstructed at the critical lethal temperature, and the synthesis of DNA, RNA and protein is influenced. The effect of ultrasound on cells mainly has thermal, cavitation and mechanical effects. The thermal effect is that when the ultrasound propagates in the medium, the cavitation bubbles generate instantaneous high temperature (about 5000 ℃) and high pressure (up to 500 × 104Pa) when being broken, and can thermally dissociate water vapor to generate OH free radicals and H atoms, and the redox reaction caused by the OH free radicals and H atoms can cause polymer degradation, enzyme inactivation, lipid peroxidation and cell killing.

Therefore, for the technology of preparing cosmetics requiring cell activity maintenance, it is important to provide a low temperature environment for the ultrasonic disruption process, so we propose an ultrasonic cell disruptor and a cell disruption process method for preparing cosmetics.

Disclosure of Invention

The invention aims to provide a cell disruptor for cosmetic preparation, which aims to solve the problems.

In order to achieve the purpose, the invention provides the following technical scheme: a cell disruption instrument for preparing cosmetics comprises a cell disruption instrument body, wherein a first cooling groove is arranged inside the cell disruption instrument body, a second cooling groove is arranged inside the first cooling groove, a cover plate is arranged at the top of the second cooling groove, an ultrasonic transducer is arranged at the top of the cover plate, an amplitude transformer is arranged at the output end of the ultrasonic transducer, an ultrasonic probe is fixedly connected at the bottom of the amplitude transformer and is positioned inside the second cooling groove, the amplitude transformer is provided with a telescopic device, supporting rods are fixedly connected at the left end and the right end of the telescopic device, the two ends of the two supporting rods are respectively and fixedly connected with the inside of the second cooling groove, a reaction container is arranged inside the second cooling groove, the first cooling groove is rotatably connected with the second cooling groove, fixing rings are fixedly connected at the upper end and the lower end of the outer surface of the first cooling groove, and connecting rings are fixedly connected at the upper end, two curved recess one, two are all seted up at go-between top both ends square recess two, four are all seted up at solid fixed ring bottom both ends two the inside gyro wheel that all is provided with of recess two, four the inside bull stick, four of all being provided with of recess two the bull stick both ends are equallyd divide do not run through four gyro wheels with two fixed connection of four recesses, four the bull stick is equallyd divide and do not run through four gyro wheels, four the gyro wheel is equallyd divide and is do not rotated with four bull sticks and be connected, cooling bath one inside is provided with cooling liquid.

Preferably, two bottom axis departments of cooling bath are provided with fixed cover, fixed cover inner wall left side is connected with connecting rod one through the bearing rotation, the one end fixedly connected with helical gear one of fixed cover is kept away from to connecting rod one, two bottom axis departments of cooling bath fixedly connected with connecting rod two, two bottom fixedly connected with helical gear two of connecting rod, be provided with helical gear three under the helical gear two, three bottom fixedly connected with connecting rods of helical gear three, cell disruption appearance body inner wall bottom is provided with the drive chamber, connecting rod three runs through cooling bath one and extends to a cooling bath outside, three bottoms of connecting rod run through the drive chamber and extend to the drive intracavity portion and be connected through bearing and drive intracavity wall rotation, connecting rod three and a cooling bath fixed connection.

Preferably, the cooling liquid is a magnetized liquid, a permanent magnetic part is arranged at the bottom of the cooling tank II, the connecting rod III penetrates through the permanent magnetic part, a fixed gear is fixedly connected to the outer surface of the connecting rod III, a rack is arranged on the left side of the fixed gear, the fixed gear is meshed with the rack, a sealing plate is fixedly connected to one end, far away from the fixed gear, of the rack, and fluid is arranged on the left side of the sealing plate.

Preferably, both ends of the top of the cooling tank are provided with ventilating openings; and the second cooling tank is made of ferromagnetic materials.

Preferably, the two fixing rings are respectively clamped with the two connecting rings, and the bottoms of the two connecting rings are fixedly connected with a support; the bottoms of the four rollers are respectively in surface contact with the four grooves.

Preferably, the fixed sleeve is fixedly connected with the bottom of the inner wall of the first cooling groove, the top of the fixed sleeve penetrates through the second cooling groove and extends to the wall of the second cooling groove, and the top of the fixed sleeve is arranged in an annular shape.

Preferably, the second bevel gear and the third bevel gear are respectively perpendicular to the first bevel gear, and are respectively meshed with the first bevel gear.

Preferably, the rack is connected with the inside of the driving cavity in a sliding mode, and the sealing plate is connected with the driving cavity in a sealing mode.

The invention also aims to provide a process method for cell disruption by adopting the cell disruptor for cosmetic preparation, which specifically comprises the following steps:

1) placing the object to be crushed in a reaction container;

2) starting the ultrasonic cell disruption instrument to perform ultrasonic disruption, and simultaneously driving the cooling liquid (12) to cool by the relative reverse rotation of the cooling tank I (2) and the cooling tank II (3);

3) after the crushing is finished, the first cooling groove (2) and the second cooling groove (3) stop rotating, and the cooling liquid (12) resets.

The invention provides a cell disruption instrument for cosmetic preparation and a cell disruption process method, and the cell disruption instrument has the following beneficial effects:

1. the cooling tank I and the cooling tank II can rotate in opposite directions by being driven to rotate by the heated evaporation of the fluid, wherein the cooling liquid moves towards the inner wall between the cooling tank I and the cell disruption instrument body under the action of centrifugal force, and the cooling liquid is magnetized liquid, demagnetizes and absorbs heat due to separation from a magnetization range, and the permanent magnetic piece is heated and heated to demagnetize, so that the cooling liquid is demagnetized and absorbs heat, and plays a cooling role in the cooling tank II, thereby ensuring the rapid cooling of the inside of the cooling tank II, avoiding the killing of cells due to high temperature, and ensuring the activity of the cells.

2. According to the invention, the first cooling tank is supported by the fixing ring and the connecting ring, so that the structural stability of the first cooling tank and the second cooling tank is ensured, and the first cooling tank can uniformly rotate in the cell crusher body by arranging the idler wheels, so that the first cooling tank and the second cooling tank can rotate in opposite directions, the practicability of the device is higher, and the device is more beneficial to rapid cooling of the second cooling tank.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged schematic view of the present invention A;

FIG. 3 is an enlarged view of the present invention B.

In the figure: 1 cell disruption instrument body, 2 cooling groove I, 3 cooling groove II, 4 ventilating openings, 5 cover plates, 6 ultrasonic transducers, 7 fixing rings, 8 connecting rings, 9 supports, 10 rollers, 11 rotating rods, 12 cooling liquid, 13 fixing sleeves, 14 connecting rods I, 15 bevel gear I, 16 connecting rods II, 17 bevel gear II, 18 bevel gear III, 19 connecting rods III, 20 permanent magnetic pieces, 21 driving cavities, 22 fixed gears, 23 racks, 24 sealing plates, 25 fluid, 26 amplitude transformer, 27 ultrasonic probes, 28 telescopic devices and 29 supporting rods.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

Examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the invention and to simplify the description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be considered limiting of the invention.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

As shown in fig. 1-3, the present invention provides a technical solution: a cell crusher for preparing cosmetics comprises a cell crusher body 1, a cooling groove I2 is arranged inside the cell crusher body 1, two ends of the top of the cooling groove I2 are respectively provided with a ventilation port 4 for isolating closed liquid so as to realize air exchange and heat exchange with external cold air, a cooling groove II 3 is arranged inside the cooling groove I2, the top of the cooling groove II 3 is provided with a cover plate 5, the top of the cover plate 5 is provided with an ultrasonic transducer 6, the output end of the ultrasonic transducer 6 is provided with an amplitude transformer 26, the bottom of the amplitude transformer 26 is fixedly connected with an ultrasonic probe 27, the ultrasonic probe 27 is positioned inside the cooling groove II 3, the amplitude transformer 26 is provided with a telescopic device 28, the left end and the right end of the telescopic device 28 are respectively and fixedly connected with supporting rods 29, two ends of the two supporting rods 29 are respectively and fixedly connected with the inside of the cooling groove II 3, the cooling groove II 3 is made of, the cooling tank I2 is rotationally connected with the cooling tank II 3, the upper end and the lower end of the outer surface of the cooling tank I2 are fixedly connected with the fixing rings 7, the upper end and the lower end of the inner wall of the cell crusher body 1 are fixedly connected with the connecting rings 8, the cooling tank I2 is supported by arranging the fixing rings 7 and the connecting rings 8, the stability of the structures of the cooling tank I2 and the cooling tank II 3 is ensured, the cooling tank I2 can uniformly rotate in the cell crusher body 1 by arranging the rolling wheels 10, so that the cooling tank I2 and the cooling tank II 3 can mutually and reversely rotate, the device has higher practicability and is more favorable for rapidly cooling the cooling tank II 3, the two fixing rings 7 are respectively clamped with the two connecting rings 8, the bottoms of the two connecting rings 8 are respectively and fixedly connected with the bracket 9, the two ends of the tops of the two connecting rings 8 are respectively provided with the arc-shaped first grooves, the two ends of the bottoms, the rollers 10 are arranged in the four second grooves, the bottoms of the four rollers 10 are respectively in surface contact with the four first grooves, the rotating rods 11 are arranged in the four second grooves, two ends of the four rotating rods 11 are respectively fixedly connected with the four second grooves, the four rotating rods 11 respectively penetrate through the four rollers 10, the four rollers 10 are respectively in rotary connection with the four rotating rods 11, the magnetizable cooling liquid 12 is arranged in the cooling groove I2, the central shaft at the bottom of the cooling groove II 3 is provided with a fixing sleeve 13, the fixing sleeve 13 is fixedly connected with the bottom of the inner wall of the cooling groove I2, the top of the fixing sleeve 13 penetrates through the cooling groove II 3 and extends to the wall of the cooling groove II 3, the top of the fixing sleeve 13 is arranged in an annular manner, the left side of the inner wall of the fixing sleeve 13 is rotatably connected with a first connecting rod 14 through a bearing, one end, far away from the fixing sleeve 13, of the, the bottom of the second connecting rod 16 is fixedly connected with a second helical gear 17, a third helical gear 18 is arranged right below the second helical gear 17, the second helical gear 17 and the third helical gear 18 are respectively and vertically arranged with the first helical gear 15, the second helical gear 17 and the third helical gear 18 are respectively and respectively meshed with the first helical gear 15, the bottom of the third helical gear 18 is fixedly connected with a third connecting rod 19, the bottom of the inner wall of the cell disruptor body 1 is provided with a driving cavity 21, the third connecting rod 19 penetrates through the first cooling groove 2 and extends to the outside of the first cooling groove 2, the bottom of the third connecting rod 19 penetrates through the driving cavity 21 and extends to the inside of the driving cavity 21 and is rotatably connected with the inner wall of the driving cavity 21 through a bearing, the third connecting rod 19 is fixedly connected with the first cooling groove 2, the bottom of the second cooling groove 3 is provided with a permanent magnet 20, the, the fixed gear 22 meshes with the rack 23, the rack 23 is kept away from the one end fixedly connected with closing plate 24 of fixed gear 22, rack 23 and the inside sliding connection of drive chamber 21, closing plate 24 and drive chamber 21 sealing connection, closing plate 24 left side is provided with fluid 25, fluid 25 is the easy evaporation fluid of being heated, it is rotatory to drive 19 through utilizing fluid 25 to be heated evaporation, thereby can make cooling bath 2 and cooling bath two 3 produce the counter rotation, wherein cooling liquid 12 is to inner wall removal between cooling bath 2 and the cell disruption appearance body 1 under the effect of centrifugal force, permanent magnetism spare 20 is heated the intensification and the demagnetization, thereby lead to cooling liquid 12 demagnetization and absorb heat, to the inside cooling performance of cooling bath two 3, thereby guaranteed the quick cooling to cooling bath two 3 insides, avoid the high temperature to cause the kill and kill of cell, the activity of cell has been guaranteed.

When the device is used, high temperature is generated when cavitation bubbles are broken, so that the fluid 25 is heated and evaporated to push the sealing plate 24 to move rightwards, the cooling groove I2 and the cooling groove II 3 can be driven to rotate reversely, the cooling liquid 12 moves towards the inner wall between the cooling groove I2 and the cell disruption instrument body 1 under the action of centrifugal force, and once the cooling liquid is separated from the magnetization range of the permanent magnetic piece 20, demagnetization can be generated to generate a heat absorption effect; meanwhile, the permanent magnet piece 20 is heated to raise the temperature and demagnetize, so that the cooling liquid 12 is demagnetized and absorbs heat, and the cooling effect is exerted on the inside of the cooling tank II 3, thereby ensuring the rapid cooling of the inside of the cooling tank II 3.

In conclusion, the invention can make the cooling tank I2 and the cooling tank II 3 rotate reversely by utilizing the rotation of the fluid 25 driven by the heat evaporation, wherein the cooling liquid 12 moves towards the inner wall between the cooling tank I2 and the cell disruption instrument body 1 under the action of centrifugal force, the permanent magnetic piece 20 is heated and heated to demagnetize, thereby causing the cooling liquid 12 to demagnetize and absorb heat, thereby playing a cooling role in the cooling tank II 3, thereby ensuring the rapid cooling in the cooling tank II 3, avoiding the cell from being killed by high temperature, ensuring the activity of the cell, supporting the cooling tank I2 by arranging the fixing ring 7 and the connecting ring 8, ensuring the stability of the structures of the cooling tank I2 and the cooling tank II 3, and making the cooling tank I2 rotate uniformly in the cell disruption instrument body 1 by arranging the roller 10, thereby making the cooling tank I2 and the cooling tank II 3 rotate reversely, the practicability of the device is higher, and the device is more favorable for quickly cooling the second cooling tank 3.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A cell disruptor for cosmetic preparation, comprising a cell disruptor body (1), characterized in that: the cell disruption instrument is characterized in that a first cooling groove (2) is arranged inside the cell disruption instrument body (1), a second cooling groove (3) is arranged inside the first cooling groove (2), a cover plate (5) is arranged at the top of the second cooling groove (3), an ultrasonic transducer (6) is arranged at the top of the cover plate (5), an amplitude transformer (26) is arranged at the output end of the ultrasonic transducer (6), an ultrasonic probe (27) is fixedly connected to the bottom of the amplitude transformer (26), the ultrasonic probe (27) is positioned inside the second cooling groove (3), a telescopic device (28) is arranged on the amplitude transformer (26), supporting rods (29) are fixedly connected to the left end and the right end of the telescopic device (28), two ends of each supporting rod (29) are fixedly connected with the inside of the second cooling groove (3), a reaction container is arranged inside the second cooling groove (3), the first cooling groove (2) is rotatably connected with the second cooling groove (3), the upper end and the lower end of the outer surface of the cooling groove I (2) are fixedly connected with fixing rings (7), the upper end and the lower end of the inner wall of the cell disruptor body (1) are fixedly connected with connecting rings (8), two ends of the top of the two connecting rings (8) are respectively provided with an arc-shaped groove I, two ends of the bottom of the two fixing rings (7) are respectively provided with a square groove II, rollers (10) are respectively arranged in the four groove II, rotating rods (11) are respectively arranged in the four groove II, two ends of the four rotating rods (11) are respectively and fixedly connected with the four groove II, the four rotating rods (11) respectively penetrate through the four rollers (10), and the four rollers (10) are respectively and rotatably connected with the four rotating rods (11); a cooling liquid (12) is arranged in the first cooling groove (2);

cooling tank two (3) bottom axis department is provided with fixed cover (13), fixed cover (13) inner wall left side is connected with connecting rod one (14) through the bearing rotation, the one end fixedly connected with helical gear one (15) of fixed cover (13) are kept away from in connecting rod one (14), cooling tank two (3) bottom axis department fixedly connected with connecting rod two (16), connecting rod two (16) bottom fixedly connected with helical gear two (17), be provided with helical gear three (18) under helical gear two (17), helical gear three (18) bottom fixedly connected with connecting rod three (19), cell disruption appearance body (1) inner wall bottom is provided with drive chamber (21), connecting rod three (19) run through cooling tank one (2) and extend to cooling tank one (2) outside, connecting rod three (19) bottom runs through drive chamber (21) and extends to drive chamber (21) inside and rotates with drive chamber (21) inner wall through the bearing and even The third connecting rod (19) is fixedly connected with the first cooling groove (2);

cooling liquid (12) are magnetizing liquid, cooling tank two (3) bottom is provided with permanent magnetism spare (20), permanent magnetism spare (20) are run through in connecting rod three (19), connecting rod three (19) surface fixedly connected with fixed gear (22), fixed gear (22) left side is provided with rack (23), fixed gear (22) and rack (23) meshing, the one end fixedly connected with closing plate (24) of fixed gear (22) are kept away from in rack (23), closing plate (24) left side is provided with fluid (25).

2. The cell disruptor for cosmetic preparation according to claim 1, characterized in that: both ends of the top of the first cooling groove (2) are provided with ventilating openings (4); the second cooling groove (3) is made of ferromagnetic materials.

3. The cell disruptor for cosmetic preparation according to claim 1, characterized in that: the two fixing rings (7) are respectively clamped with the two connecting rings (8), and the bottoms of the two connecting rings (8) are fixedly connected with a support (9); the bottoms of the four rollers (10) are respectively in surface contact with one surface of the four grooves.

4. The cell disruptor for cosmetic preparation according to claim 1, characterized in that: fixed cover (13) and cooling bath (2) inner wall bottom fixed connection, fixed cover (13) top runs through cooling bath two (3) and extends to cooling bath two (3) cell walls, fixed cover (13) top is the annular setting.

5. The cell disruptor for cosmetic preparation according to claim 1, characterized in that: the second bevel gear (17) and the third bevel gear (18) are respectively perpendicular to the first bevel gear (15), and the second bevel gear (17) and the third bevel gear (18) are respectively meshed with the first bevel gear (15).

6. The cell disruptor for cosmetic preparation according to claim 1, characterized in that: the rack (23) is connected with the interior of the driving cavity (21) in a sliding mode, and the sealing plate (24) is connected with the driving cavity (21) in a sealing mode.

7. A cell disruption process for cosmetic preparation, which is characterized by comprising the following steps: a method for cell disruption using the cell disruptor for cosmetic preparation according to any of claims 1 to 6, comprising:

1) placing the object to be crushed in a reaction container;