CN111732951A - 一种氮掺杂绿色荧光碳量子点及其制备方法和应用 - Google Patents

一种氮掺杂绿色荧光碳量子点及其制备方法和应用 Download PDF

Info

Publication number
CN111732951A
CN111732951A CN202010640042.7A CN202010640042A CN111732951A CN 111732951 A CN111732951 A CN 111732951A CN 202010640042 A CN202010640042 A CN 202010640042A CN 111732951 A CN111732951 A CN 111732951A
Authority
CN
China
Prior art keywords
carbon quantum
green fluorescent
nitrogen
fluorescent carbon
quantum dots
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN202010640042.7A
Other languages
English (en)
Other versions
CN111732951B (zh
Inventor
闫娅楠
宋胜梅
孟雅婷
张慧林
双少敏
董川
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanxi University
Original Assignee
Shanxi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanxi University filed Critical Shanxi University
Priority to CN202010640042.7A priority Critical patent/CN111732951B/zh
Publication of CN111732951A publication Critical patent/CN111732951A/zh
Application granted granted Critical
Publication of CN111732951B publication Critical patent/CN111732951B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
    • C09K11/65—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials containing carbon
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y40/00—Manufacture or treatment of nanostructures
    • C—CHEMISTRY; METALLURGY
    • C01—INORGANIC CHEMISTRY
    • C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
    • C01B32/00—Carbon; Compounds thereof
    • C01B32/15—Nano-sized carbon materials
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
    • C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
    • C09K11/08—Luminescent, e.g. electroluminescent, chemiluminescent materials containing inorganic luminescent materials
    • C09K11/0883—Arsenides; Nitrides; Phosphides
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6402—Atomic fluorescence; Laser induced fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N21/643—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" non-biological material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6432—Quenching

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nanotechnology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Optics & Photonics (AREA)
  • Materials Engineering (AREA)
  • Inorganic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pathology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Condensed Matter Physics & Semiconductors (AREA)
  • Manufacturing & Machinery (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

本发明提供一种氮掺杂绿色荧光碳量子点及其制备方法和应用。制备方法：将多巴胺和乙二胺溶于水中，在170～200℃下反应6～10小时以合成绿色荧光碳点。该方法制备碳点工艺简单，原料来源广泛且价格便宣，制备条件要求低。所制备的碳量子点可作为荧光探针用于比色检测姜黄素。

Description

一种氮掺杂绿色荧光碳量子点及其制备方法和应用
技术领域
本发明涉及碳发光纳米材料，尤其涉及碳量子点，具体是提供一种氮掺杂绿色荧光碳量子点及其制备方法和应用。
背景技术
姜黄素(curcumin,Cur)，1,7-双-(4-羟基-3-甲氧基苯基)-1,6-庚二烯-2,5-二酮，是一种天然多酚色素，来自姜黄的粉状根茎。由于其生物学和药理学活性，如抗氧化，抗癌，抗炎，抗菌和抗糖尿病作用，因此Cur引起了极大的关注。目前用于测定Cur的常规分析方法包括伏安法，高性能薄层层析(HPTLC)，高效液相色谱(HPLC)，超高效液相色谱(UHPLC)，液相色谱-串联质谱(LC-MS/MS)联用和分光光度法。这些方法虽然具有良好的准确性和选择性，然而样品预处理复杂、操作程序繁琐、仪器昂贵、分析时间长等缺点，限制了其应用。而荧光分光光度法由于其高精度，优良的选择性和灵敏度，操作简便，经济快速等特点，已逐渐成为一种高效的检测方法。碳点由于制备方法简便，强烈的光致发光性能、优良的稳定性、水溶性和高选择性等优点，被广泛应用于荧光探针和生物成像等领域。另外比色法具有信号易输出、响应快、操作简单等优点，可实现分析物现场便携检测，但是利用荧光比色法检测姜黄素的文献鲜有报道。因此，制备检出限低、能够比色检测姜黄素的碳点是一种挑战。
发明内容
本发明的目的在于提供一种氮掺杂绿色荧光碳量子点及其制备方法，以及将该碳量子点用于比色检测姜黄素。所述碳点制备工艺简单，原料来源广泛且价格便宣，制备条件要求低且环境友好，在一般实验室均能合成，易于推广。
本发明提供的一种氮掺杂绿色荧光碳量子点的制备方法，包括如下步骤：
将多巴胺和乙二胺混合，加入去离子水；并将该溶液转移至高压釜中，并在170～200℃下反应6～10小时；冷却至室温后，将产物用滤纸过滤除去大分子颗粒，得到的溶液通过500Da的透析膜在去离子水中透析6h；最后，将其真空干燥以收集目标碳量子点；所述的多巴胺、乙二胺和去离子水的质量比为0.01-0.2：1.00-2.00：20。
所述的多巴胺、乙二胺和去离子水的质量比优选0.01-0.1：1.50-2.00：20，更优选0.08-0.1：1.80-2.00：20。
所述的反应温度优选为180～200℃，反应时间优选为9～10小时。
上述方法制备的碳量子点作为荧光探针可用于比色检测姜黄素的检测，根据公式cmin＝3Sb/S求出最低检出限为7.887nM，线性范围0.08-185.68uM。
本发明的优点与效果是：
本发明通过一步水热法即可得到碳量子点溶液，合成方法简单有效，原料廉价易得，反应条件温和且环境友好，在一般实验室均能完成，易于推广。所制备的碳量子点作为探针可用于比色检测姜黄素。
附图说明
图1为实施例1制备的碳量子点的紫外吸收光谱及荧光发射光谱。
图2为实施例1制备的碳量子点的红外光谱图，图中横坐标为检测波长，纵坐标为透过率。
图3为姜黄素淬灭实施例1制备的碳量子点的荧光光谱图。
图4为姜黄素的浓度在0.08-185.68uM范围内的线性拟合曲线。
图5为实施例1制备的碳量子点荧光发射曲线随激发波长变化的光谱图。
图6为实施例1制备的碳量子点用于比色检测姜黄素的图。
具体实施方式
下面结合附图以及具体实施例对本发明做出进一步说明，实施例给出了详细的实施方式和具体的操作过程，但本发明的保护范围不限于下述的实施例。
实施例1
步骤1，室温下，将0.1000g的多巴胺和2mL乙二胺溶于20ml水中，充分搅拌，超声得到澄清溶液。
步骤2，将溶液转移至50ml水热反应釜中。
步骤3，将水热釜置于烘箱中，200℃反应10小时。
步骤4，将产物用滤纸过滤除去大分子颗粒，得到的溶液通过500Da的透析膜在去离子水中透析6h。
步骤5，将上述荧光碳量子点水溶液冷冻干燥后得到荧光碳量子点，其相对量子产率(以硫酸奎宁为标准)为1.6％。
对所制备的荧光碳量子点进行表征：
上述制备的碳量子点的紫外吸收光谱及荧光发射光谱，见图1；
上述制备的碳量子点的红外光谱图见图2，图中横坐标为检测波长，纵坐标为透过率；
用姜黄素淬灭上述制备的碳量子点的荧光光谱见图3；
姜黄素的浓度在0.08-185.68uM范围内的线性拟合曲线见图4；
上述制备的碳量子点荧光发射曲线随激发波长变化的光谱见图5。
实施例2
除四硼酸钠的质量为0.0600g，其它均与实施例1相同。其相对量子产率(以硫酸奎宁为标准)为0.52％。
实施例3
除四硼酸钠的质量为0.0800g，其它均与实施例1相同。其相对量子产率(以硫酸奎宁为标准)为1.02％。
实施例4
除反应时间为180℃10小时，其它均与实施例1相同。其相对量子产率(以硫酸奎宁为标准)为0.97％。
实施例5
取1mL二次水与1mL的实施例1制备的碳量子点水溶液混合，在线性范围内加入不同浓度的姜黄素溶液，在日光下可以看到溶液颜色的改变。因此，可以用于比色检测姜黄素。

Claims (6)

1.一种氮掺杂绿色荧光碳量子点的制备方法，其特征在于，包括如下步骤：
将多巴胺和乙二胺混合，加入去离子水；并将该溶液转移至高压釜中，并在170～200℃下反应6～10小时；冷却至室温后，将产物用滤纸过滤除去大分子颗粒，得到的溶液通过500Da的透析膜在去离子水中透析6h；最后，将其真空干燥以收集目标碳量子点；所述的多巴胺、乙二胺和去离子水的质量比为0.01-0.2：1.00-2.00：20。
2.如权利要求1所述的氮掺杂绿色荧光碳量子点的制备方法，其特征在于，所述的多巴胺、乙二胺和去离子水的质量比为0.01-0.1：1.50-2.00：20。
3.如权利要求2所述的氮掺杂绿色荧光碳量子点的制备方法，其特征在于，所述的多巴胺、乙二胺和去离子水的质量比为0.08-0.1：1.80-2.00：20。
4.如权利要求1所述的氮掺杂绿色荧光碳量子点的制备方法，其特征在于，所述的反应温度为180～200℃，反应时间为9～10小时。
5.如权利要求1-4任一项所述方法制备的氮掺杂绿色荧光碳量子点。
6.如权利要求5所述的氮掺杂绿色荧光碳量子点用于比色检测姜黄素。
CN202010640042.7A 2020-07-06 2020-07-06 一种氮掺杂绿色荧光碳量子点及其制备方法和应用 Active CN111732951B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202010640042.7A CN111732951B (zh) 2020-07-06 2020-07-06 一种氮掺杂绿色荧光碳量子点及其制备方法和应用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202010640042.7A CN111732951B (zh) 2020-07-06 2020-07-06 一种氮掺杂绿色荧光碳量子点及其制备方法和应用

Publications (2)

Publication Number Publication Date
CN111732951A true CN111732951A (zh) 2020-10-02
CN111732951B CN111732951B (zh) 2022-05-20

Family

ID=72653380

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202010640042.7A Active CN111732951B (zh) 2020-07-06 2020-07-06 一种氮掺杂绿色荧光碳量子点及其制备方法和应用

Country Status (1)

Country Link
CN (1) CN111732951B (zh)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116790248A (zh) * 2023-06-06 2023-09-22 江南大学 一种锌氮掺杂碳点及其制备方法和应用

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120134938A1 (en) * 2010-11-30 2012-05-31 Perricone Nicholas V Melanin Promoting Topical Composition
US20150018294A1 (en) * 2012-02-07 2015-01-15 Arrigo DE BENEDETTI Modulators of tousled kinase in cellular processes
US20170299502A1 (en) * 2016-04-19 2017-10-19 Ariana Schanzer Contaminant monitor system and method
CN109507160A (zh) * 2018-11-21 2019-03-22 山西大学 一种快速检测姜黄素的试纸和方法
CN109929550A (zh) * 2019-04-16 2019-06-25 山西大学 一种氮掺杂蓝色荧光碳量子点及其制备方法和应用
WO2019173338A1 (en) * 2018-03-07 2019-09-12 Reform Biologics, Llc Excipient compounds for protein formulations
CN111122522A (zh) * 2019-12-05 2020-05-08 山西大学 “开关”型顺序检测姜黄素和铕离子的荧光探针及其制备方法和应用

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120134938A1 (en) * 2010-11-30 2012-05-31 Perricone Nicholas V Melanin Promoting Topical Composition
US20150018294A1 (en) * 2012-02-07 2015-01-15 Arrigo DE BENEDETTI Modulators of tousled kinase in cellular processes
US20170299502A1 (en) * 2016-04-19 2017-10-19 Ariana Schanzer Contaminant monitor system and method
WO2019173338A1 (en) * 2018-03-07 2019-09-12 Reform Biologics, Llc Excipient compounds for protein formulations
CN109507160A (zh) * 2018-11-21 2019-03-22 山西大学 一种快速检测姜黄素的试纸和方法
CN109929550A (zh) * 2019-04-16 2019-06-25 山西大学 一种氮掺杂蓝色荧光碳量子点及其制备方法和应用
CN111122522A (zh) * 2019-12-05 2020-05-08 山西大学 “开关”型顺序检测姜黄素和铕离子的荧光探针及其制备方法和应用

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GUANGMING LI等: "Nitrogen-doped carbon dots as a fluorescent probe suitable for sensing Fe3+ under acidic conditions", 《ROYAL SOCIETY OF CHEMISTRY》 *
YANAN YAN等: "N-Doped carbon dots for the fluorescence and", 《ROYAL SOCIETY OF CHEMISTRY》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116790248A (zh) * 2023-06-06 2023-09-22 江南大学 一种锌氮掺杂碳点及其制备方法和应用
CN116790248B (zh) * 2023-06-06 2023-12-12 江南大学 一种锌氮掺杂碳点及其制备方法和应用

Also Published As

Publication number Publication date
CN111732951B (zh) 2022-05-20

Similar Documents

Publication Publication Date Title
US10900899B1 (en) Method for preparing ratiometric fluorescent probe for cymoxanil based on double-emission quantun dot-silver nanoparticle complex
CN108047060B (zh) 一种识别检测甲醛的芘衍生物荧光探针分子及其制备方法和应用
CN109081836B (zh) 一种基于半花菁结构的汞离子近红外荧光探针及其制备方法和应用
CN108485661B (zh) 一种荧光碳量子点的制备方法及其应用
CN108611090A (zh) 一种荧光碳量子点及其制备方法和应用
CN111732951B (zh) 一种氮掺杂绿色荧光碳量子点及其制备方法和应用
CN106833628A (zh) 表面修饰的碳纳米点及其制备和作为荧光探针检测Cu2+及谷胱甘肽的应用
CN109206351B (zh) 一种基于花菁结构测钯离子的近红外荧光探针、其制备方法及应用
CN109929550A (zh) 一种氮掺杂蓝色荧光碳量子点及其制备方法和应用
CN111286321B (zh) 一种黄酮类荧光纳米材料的制备方法及其应用
CN111205220B (zh) 一种荧光探针及其制备方法和应用
CN110642857B (zh) 一种用于检测粘度和pH的双功能荧光探针及其制备与应用
CN113979890B (zh) 一种席夫碱配体及其多核稀土配合物的制备方法和应用
CN111925792A (zh) 一种红色荧光碳量子点、和其制备方法及应用
CN110317175B (zh) 一种萘衍生物及其合成方法和应用
CN109735333B (zh) 一种掺铕钼酸钙粉体的制备方法及应用
CN108913124B (zh) 一种壳聚糖衍生物p在检测铝离子中的应用
CN111187618B (zh) 一种橘色荧光碳量子点及其制备方法和应用
CN1426415A (zh) 荧光化合物
CN107314979A (zh) 一种基于罗丹明6g的铜离子传感器、制备及应用
CN113493473A (zh) 一种高效红光铕配合物发光材料及其制备方法和应用
CN112881350A (zh) 以杨桃为碳源的碳量子点的制备方法及其在铜离子检测中的应用
CN110467570A (zh) 一种四苯乙烯-8-羟基喹啉类化合物及其制备方法和应用
CN110776647A (zh) 一种基于铕金属有机框架的比率探针及其构建方法和应用
CN109180694A (zh) 一种用于细胞成像的罗丹明衍生物荧光探针及其制备方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant