CN111727967A - Preparation method of compound insect sex pheromone for preventing and controlling rice field pests - Google Patents
Preparation method of compound insect sex pheromone for preventing and controlling rice field pests Download PDFInfo
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- CN111727967A CN111727967A CN202010443094.5A CN202010443094A CN111727967A CN 111727967 A CN111727967 A CN 111727967A CN 202010443094 A CN202010443094 A CN 202010443094A CN 111727967 A CN111727967 A CN 111727967A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
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Abstract
The invention discloses a preparation method of compound insect sex pheromone for preventing and controlling rice field pests. The composition of the compound insect sex pheromone comprises a first type sex pheromone, a second type sex pheromone and a third type sex pheromone; the mass ratio of the first-class sex pheromone to the second-class sex pheromone to the third-class sex pheromone is (2-5): (1-2): (2-3); the preparation method comprises the following steps: (1) preparing a composition reagent, mixing a synergist into a second-class sex pheromone solution under the condition of standard atmospheric pressure and 20-25 ℃, mixing the first-class sex pheromone, the second-class sex pheromone and the third-class sex pheromone according to a proportion, standing for later use; (2) and sequentially mixing the antioxidant and the light stabilizer in a certain proportion into the composition reagent, and uniformly mixing to obtain the compound insect sex pheromone reagent. The composition reagent can be used for preventing and controlling pests in rice fields, and has the advantages of simultaneously luring three types of pests to gather and greatly improving the efficiency of preventing and controlling the pests in a specific area.
Description
Technical Field
The invention relates to the field of pest control reagents, in particular to a preparation method of a compound insect sex pheromone for controlling rice field pests.
Background
The pests in the rice field are always important factors influencing the mu yield of the rice field, and along with the development and the progress of science and technology, the application of sex pheromone to control the pests becomes an important technology in the field of pest control.
Sex pheromone products for controlling pests generally comprise a lure and a trap, wherein the lure is the sex pheromone for attracting specific types of pests, and the trap is a container for containing the sex pheromone. The working principle of the product is as follows: the high-concentration bionic synthetic smell peculiar to female pests is released through the inducement so as to attract the male pests to gather in a certain range around the trap. The normal positioning of the male worms on the female worms is interfered, so that the normal mating and spawning of the male worms and the female worms are reduced, the output of worm eggs is reduced, and the effect of reducing the number of pests is achieved. In practical application, the periphery of the trap can be provided with an intelligent insect killing lamp, an insect sticking plate and other insect killing devices so as to accelerate the killing rate of attracted males and further accelerate the reduction rate of the number of pests.
The existing striped rice borer sex pheromone composition with publication number CN101617652A and the induction thereof are characterized in that sex pheromones of cis 11-hexadecenal, cis 13-octadecenal, Z9-hexadecenal and hexadecenal are used in cooperation with a releaser to attract male striped rice borers, so that the mating and oviposition frequency of the striped rice borers in a paddy field is reduced.
A sex pheromone composition for attracting prodenia litura and an attracting core thereof, which are disclosed in the prior publication No. CN101940208A, attract male prodenia litura by sex pheromones with the components of cis 9 trans 11-tetradecadiene acetate and cis 9 trans 12-tetradecadiene acetate and matching with a synergist taking phenylacetaldehyde as a raw material, so as to reduce pests in vegetable lands adjacent to a rice field.
The sex pheromone composition for luring citrus leaf miner and the luring cause thereof with the prior publication number of CN102369925A attract male citrus leaf miner through sex pheromones which comprise cis 7 cis 11 trans 13-hexadecadienal, cis 7 cis 11-hexadecadienal and cis 7-hexadecadienal, so as to reduce pests in forest gardens adjacent to rice fields.
The above prior art solutions have the following drawbacks: the common sex pheromone can only attract a specific type of pest, and the requirement for simultaneously attracting a plurality of types of pest is difficult to meet, so that improvement is needed.
Disclosure of Invention
Aiming at the defects in the prior art, the first purpose of the invention is to provide the compound insect sex pheromone for preventing and controlling the pests in the paddy field, which has the advantages of simultaneously luring three types of pests to gather and greatly improving the efficiency of preventing and controlling the pests in a specific area.
The second purpose of the invention is to provide the compound insect sex pheromone for preventing and controlling the pests in the paddy field, which has the advantages of simple preparation method and easy operation.
In order to achieve the first object, the invention provides the following technical scheme: a compound insect sex pheromone for preventing and controlling pests in paddy field, the composition of the compound insect sex pheromone comprises a first type sex pheromone, a second type sex pheromone and a third type sex pheromone;
the sex pheromone comprises the following components in parts by weight: 4-6 parts of cis-11-hexadecenal, 3-4 parts of cis-13-octadecenal, 5-18 parts of cis-9-hexadecenal and 1-6 parts of hexadecenal;
the second type of sex pheromone comprises the following components in parts by weight: 3-8 parts of cis-9 trans-11-tetradecadiene acetate and 5-11 parts of cis-9 trans-12-tetradecadiene acetate;
the three types of sex pheromones comprise the following components in parts by weight: 2-5 parts of cis 7 cis 11 trans 13-hexadecadienal, 3-8 parts of cis 7 cis 11-hexadecadienal and 4-9 parts of cis 7-hexadecenoic acid;
the mass ratio of the first-class sex pheromone to the second-class sex pheromone to the third-class sex pheromone is (2-5): (1-2): (2-3).
By adopting the technical scheme, the compound insect sex pheromone is prepared by mixing the first-class sex pheromone, the second-class sex pheromone and the third-class sex pheromone, and can be used for trapping chilo suppressalis, prodenia litura and citrus leaf miner which correspond to the three-class sex pheromone respectively, so that the three types of pests in the rice field and the surrounding environment of the rice field are reduced, and the acre yield of grains in the rice field is effectively guaranteed. The weight parts of the components of the first-class sex pheromone, the second-class sex pheromone and the third-class sex pheromone in the respective sex pheromones can be adjusted, and the conclusion can be drawn by comparing the data of a plurality of groups of experiments: the sex pheromone components of all kinds are selected and matched according to the weight part ratio of the middle value, so that the trapping effect of the sex pheromone on pests can be maximized; when the weight parts of the components in the first type of sex pheromone are intermediate values, the trapping effect of the second type of sex pheromone and the third type of sex pheromone can be effectively improved; when the components in the second type sex pheromone and the third type sex pheromone are selected from the middle value weight part, the composition has good effect only on self-trapped pests, and has no promoting effect on other types of sex pheromones; when the weight ratio of the first type sex pheromone, the second type sex pheromone and the third type sex pheromone is equal, the composition reagent can exert the optimal trapping effect. Therefore, when an operator uniformly mixes the first-class sex pheromone, the second-class sex pheromone and the third-class sex pheromone in equal parts by weight and the weight parts of the components in each sex pheromone are intermediate values of the corresponding interval, the composite insect sex pheromone can simultaneously exert the optimal trapping effect on the three types of insects, can efficiently accumulate male pests in a rice field and the surrounding environment thereof, effectively reduce the mating and spawning of male insects and female insects, and further achieve the effect of improving the pest control efficiency of the rice field.
Further, the second type of sex pheromone also comprises a synergist, and the synergist is phenylacetaldehyde.
By adopting the technical scheme, because the number of the components in the second type of sex pheromone is less, compared with the first type of sex pheromone and the second type of sex pheromone, the second type of sex pheromone has the characteristics of shorter use time and less effect; by adding the synergist, the second type of sex pheromone has stronger trapping attraction to prodenia litura, so that the use synchronism of the three types of sex pheromones and the use stability of the compound insect sex pheromone are ensured.
Further, the synergist accounts for 15% of the total mass of the second type of sex pheromone.
By adopting the technical scheme, the cliff type fall occurs to the trapping quantity of the two types of sex pheromones without adding the synergist to the prodenia litura, and meanwhile, the trapping effect of the other two types of sex pheromones is slightly reduced, so that the addition of the synergist can ensure and improve the use stability of the compound insect sex pheromone.
Further, the composition also comprises an antioxidant, wherein the antioxidant accounts for 8% of the total mass of the composition.
By adopting the technical scheme, the antioxidant can effectively improve the stability of the composition reagent in the composition reagent, and the compound insect sex pheromone without the antioxidant has cliff-breaking drop on the trapping number of three types of pests, so that the trapping effect of each sex pheromone on the pests is greatly influenced, the overall effect of the compound insect sex pheromone is greatly reduced, and the trapping effect of the compound insect sex pheromone on the pests can be greatly improved by adding the antioxidant.
Further, the antioxidant is one or more of hindered phenols, phosphorous acids and thioesters.
By adopting the technical scheme, the trapping effect of the compound insect sex pheromone on pests can be improved by selecting any one of hindered phenol, phosphorous acid or thioester antioxidants, and when an operator uses any two of the three or more antioxidants, the operator can obtain corresponding effects; therefore, when the laboratory conditions are limited, the operator can select any one of the three types optionally, and further the experiment progress is guaranteed.
Further, the composition also comprises a light stabilizer, wherein the light stabilizer accounts for 6% of the total mass of the composition.
By adopting the technical scheme, the light stabilizer can effectively reduce the component failure phenomenon of the composition reagent after being irradiated by ultraviolet rays, and effectively ensures the self-stability of the composition reagent; the compound insect sex pheromone without adding the light stabilizer has cliff type falling on the trapping number of three types of pests, and the trapping effect of the compound insect sex pheromone reagent on the pests is greatly reduced, so that the light stabilizer is very necessary to be added into the composition reagent.
Further, the light stabilizer is a hindered amine light stabilizer.
By adopting the technical scheme, the hindered amine light stabilizer is known, has multiple obtaining ways and has the effect of stable self property, so that the hindered amine light stabilizer is selected as the light stabilizer, the using effect of the compound insect sex pheromone can be ensured, the difficulty of reagent preparation of operators can be reduced, and the experimental progress of the compound insect sex pheromone can be improved.
In order to achieve the second object, the invention provides the following technical scheme: a preparation method of compound insect sex pheromone for preventing and controlling rice field pests comprises the following steps:
(1) preparing a composition reagent, mixing a synergist into a second-class sex pheromone solution under the condition of standard atmospheric pressure and 20-25 ℃, mixing the first-class sex pheromone, the second-class sex pheromone and the third-class sex pheromone according to a proportion, standing for later use;
(2) and sequentially mixing the antioxidant and the light stabilizer in a certain proportion into the composition reagent, and uniformly mixing to obtain the compound insect sex pheromone reagent.
Further, in the step (1), the standing time of the composition reagent is 30min-2 h.
By adopting the technical scheme, after the composition reagent is prepared and stands for 1 hour, the trapping number of the composition reagent to three types of pests is far more than that of the composition reagent under other standing time in the using process, so that the using effect of the composition reagent is optimal when the standing time of the composition is 1 hour.
Further, in the step (2), the stirring frequency of the composition reagent is 15-30r/min, and the stirring time is 5-10 min.
By adopting the technical scheme, when the stirring speed of the composition reagent is too high, the effect of trapping the composition reagent on pests can be reduced; when the stirring time of the composition reagent is less than 5min or more than 10min, the trapping number of the composition reagent to pests also falls back greatly, so that the trapping effect of the composition reagent to three types of pests can be effectively improved when the stirring time of the composition reagent is controlled between 5min and 10 min.
In conclusion, the invention has the following beneficial effects:
the first, the invention adopts the method that the three sex pheromones are mixed to prepare the compound insect sex pheromone, because the first, second and third sex pheromones in the compound insect sex pheromone can trap the chilo suppressalis, prodenia litura and citrus leaf miner which correspond to the first, the third kind of pests in the paddy field and the surrounding environment are reduced, and the acre yield output of the grain in the paddy field is effectively ensured; meanwhile, when the sex pheromone components of all kinds are selected and matched according to the weight part ratio at the middle value, the trapping effect of the sex pheromone on pests can be maximized; when the weight parts of the components in the first type of sex pheromone are intermediate values, the trapping effect of the second type of sex pheromone and the third type of sex pheromone can be effectively improved; when the components in the second type sex pheromone and the third type sex pheromone are selected from the middle value weight part, the composition has good effect only on self-trapped pests, and has no promoting effect on other types of sex pheromones; when the weight ratio of the first type sex pheromone, the second type sex pheromone and the third type sex pheromone is equal, the composition reagent can exert the optimal trapping effect. Therefore, when an operator uniformly mixes the first-class sex pheromone, the second-class sex pheromone and the third-class sex pheromone in equal parts by weight and the weight parts of the components in each sex pheromone are intermediate values of the corresponding interval, the composite insect sex pheromone can simultaneously exert the optimal trapping effect on the three types of insects, can efficiently accumulate male pests in the rice field and the surrounding environment thereof, effectively reduce the mating and oviposition of male insects and female insects, and further achieve the effect of improving the pest control efficiency of the rice field;
secondly, the stabilizing agent is added into the second type of sex pheromone, so that the second type of sex pheromone has stronger trapping attraction to prodenia litura, and the use synchronism of the three types of sex pheromones and the use stability of the compound insect sex pheromone are further ensured; the trapping effect of the composition agent on three types of pests is improved and the use stability of the composition agent is guaranteed by adding the light stabilizer and the antioxidant into the composition agent.
Detailed Description
The following examples further illustrate the invention in detail.
The hindered phenolic antioxidant is selected from Basf B215 produced by Kettin chemical industry, the phosphorous antioxidant is selected from Doverphos8 produced by U.S. Dover company, the thioester antioxidant is selected from DLTP produced by Hebei Baipin Biotechnology Limited, the hindered amine light stabilizer is selected from 622 light stabilizer produced by Nanjing Huaming chemical industry Limited, and the phenylacetaldehyde is selected from phenylacetaldehyde produced by Zheng Achim chemical industry Limited.
Example 1
A compound insect sex pheromone for preventing and controlling rice field pests comprises 2 parts of first-class sex pheromone, 1 part of second-class sex pheromone and 2 parts of third-class sex pheromone, and the preparation method specifically comprises the following steps:
(1) adding cis-11-hexadecenal, cis-13-octadecenal, cis-9-hexadecenal and hexadecenal into a test tube to obtain the sex pheromone. Adding cis 9 trans 11-tetradecadiene acetate and cis 9 trans 12-tetradecadiene acetate into test tube to obtain two kinds of sex pheromone. Adding cis 7 cis 11 trans 13-hexadecadienal, cis 7 cis 11-hexadecadienal and cis 7-hexadecenoic acid into test tube to obtain three kinds of sex pheromones.
Adding a synergist accounting for 15 percent of the total weight of the second type of sex pheromone into the second type of sex pheromone, mixing 2 parts of the first type of sex pheromone, 1 part of the second type of sex pheromone and 2 parts of the third type of sex pheromone, and standing for 30min for later use.
(2) The antioxidant was added to the composition reagent, the light stabilizer was then added, and the mixture was stirred for 5min at a speed of 15r/min using a glass rod to obtain the final composition reagent.
Examples 2 to 7
TABLE 1 Components and weight ratios of reagents of compositions of examples 1-6
| Component/only | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 | Example 6 |
| Cis 11-hexadecenal | 4 | 4 | 4 | 4 | 4 | 4 |
| Cis 13-octadecenal | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis-9-hexadecenal | 5 | 5 | 5 | 5 | 5 | 5 |
| Hexadecanal | 1 | 2 | 3 | 4 | 5 | 6 |
| Cis 9 trans 11-tetradecadieneacetic acid ester | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis 9 trans 12-tetradecadieneacetic acid ester | 5 | 5 | 5 | 5 | 5 | 5 |
| Cis 7 cis 11 trans 13-hexadecadienal | 2 | 2 | 2 | 2 | 2 | 2 |
| Cis 7 cis 11-hexadecadienal | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis 7-hexadecenoic acid | 4 | 4 | 4 | 4 | 4 | 4 |
| Synergist | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 |
| Antioxidant agent | 2.4 | 2.48 | 2.56 | 2.64 | 2.72 | 2.8 |
| Light stabilizers | 1.8 | 1.86 | 1.92 | 1.98 | 2.04 | 2.1 |
Examples 7 to 12
TABLE 2 Components and weight ratios of reagents of compositions of examples 7-12
| Component/only | Example 7 | Example 8 | Example 9 | Example 10 | Example 11 | Example 12 |
| Cis 11-hexadecenal | 4 | 4 | 4 | 4 | 4 | 4 |
| Cis 13-octadecenal | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis-9-hexadecenal | 5 | 5 | 5 | 5 | 5 | 5 |
| Hexadecanal | 1 | 1 | 1 | 1 | 1 | 1 |
| Cis 9 trans 11-tetradecadieneacetic acid ester | 3 | 4 | 5 | 6 | 7 | 8 |
| Cis 9 trans 12-tetradecadieneacetic acid ester | 5 | 5 | 5 | 5 | 5 | 5 |
| Cis 7 cis 11 trans 13-hexadecadienal | 2 | 2 | 2 | 2 | 2 | 2 |
| Cis 7 cis 11-hexadecadienal | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis 7-hexadecenoic acid | 4 | 4 | 4 | 4 | 4 | 4 |
| Synergist | 1.2 | 1.35 | 1.5 | 1.65 | 1.8 | 1.95 |
| Antioxidant agent | 2.4 | 2.48 | 2.56 | 2.64 | 2.72 | 2.8 |
| Light stabilizers | 1.8 | 1.86 | 1.92 | 1.98 | 2.04 | 2.1 |
Examples 13 to 18
TABLE 3 Components and weight ratios of reagents of compositions of examples 13-18
| Component/only | Example 13 | Example 14 | Example 15 | Example 16 | Example 17 | Example 18 |
| Cis 11-hexadecenal | 4 | 4 | 4 | 4 | 4 | 4 |
| Cis 13-octadecenal | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis-9-hexadecenal | 5 | 5 | 5 | 5 | 5 | 5 |
| Hexadecanal | 1 | 1 | 1 | 1 | 1 | 1 |
| Cis 9 trans 11-tetradecadieneacetic acid ester | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis 9 trans 12-tetradecadieneacetic acid ester | 5 | 5 | 5 | 5 | 5 | 5 |
| Cis 7 cis 11 trans 13-hexadecadilAlkenals | 2 | 2 | 2 | 2 | 2 | 2 |
| Cis 7 cis 11-hexadecadienal | 3 | 4 | 5 | 6 | 7 | 8 |
| Cis 7-hexadecenoic acid | 4 | 4 | 4 | 4 | 4 | 4 |
| Synergist | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 |
| Antioxidant agent | 2.4 | 2.48 | 2.56 | 2.64 | 2.72 | 2.8 |
| Light stabilizers | 1.8 | 1.86 | 1.92 | 1.98 | 2.04 | 2.1 |
Example 19
A compound insect sex pheromone for preventing and controlling rice field pests comprises 2 parts of first-class sex pheromone, 2 parts of second-class sex pheromone and 2 parts of third-class sex pheromone, and the preparation method specifically comprises the following steps:
(1) adding cis-11-hexadecenal, cis-13-octadecenal, cis-9-hexadecenal and hexadecenal into a test tube to obtain the sex pheromone. Adding cis 9 trans 11-tetradecadiene acetate and cis 9 trans 12-tetradecadiene acetate into test tube to obtain two kinds of sex pheromone. Adding cis 7 cis 11 trans 13-hexadecadienal, cis 7 cis 11-hexadecadienal and cis 7-hexadecenoic acid into test tube to obtain three kinds of sex pheromones.
Adding a synergist accounting for 15 percent of the total weight of the second type of sex pheromone into the second type of sex pheromone, mixing 2 parts of the first type of sex pheromone, 1 part of the second type of sex pheromone and 2 parts of the third type of sex pheromone, and standing for 30min for later use.
(2) The antioxidant was added to the composition reagent, the light stabilizer was then added, and the mixture was stirred for 5min at a speed of 15r/min using a glass rod to obtain the final composition reagent.
Example 20
A compound insect sex pheromone for preventing and controlling rice field pests comprises 2 parts of first-class sex pheromone, 1 part of second-class sex pheromone and 3 parts of third-class sex pheromone, and the preparation method specifically comprises the following steps:
(1) adding cis-11-hexadecenal, cis-13-octadecenal, cis-9-hexadecenal and hexadecenal into a test tube to obtain the sex pheromone. Adding cis 9 trans 11-tetradecadiene acetate and cis 9 trans 12-tetradecadiene acetate into test tube to obtain two kinds of sex pheromone. Adding cis 7 cis 11 trans 13-hexadecadienal, cis 7 cis 11-hexadecadienal and cis 7-hexadecenoic acid into test tube to obtain three kinds of sex pheromones.
Adding a synergist accounting for 15 percent of the total weight of the second type of sex pheromone into the second type of sex pheromone, mixing 2 parts of the first type of sex pheromone, 1 part of the second type of sex pheromone and 2 parts of the third type of sex pheromone, and standing for 30min for later use.
(2) The antioxidant was added to the composition reagent, the light stabilizer was then added, and the mixture was stirred for 5min at a speed of 15r/min using a glass rod to obtain the final composition reagent.
TABLE 4 dosage and weight ratio of composition reagents of examples 19-20
| Example 20 parts by weight | Example 21 parts by weight | |
| Sex pheromone | 2 | 2 |
| Sex pheromone class II | 2 | 1 |
| Three kinds of sex pheromones | 2 | 3 |
Example 21
A compound insect sex pheromone for preventing and controlling rice field pests comprises 2 parts of first-class sex pheromone, 1 part of second-class sex pheromone and 2 parts of third-class sex pheromone, and the preparation method specifically comprises the following steps:
(1) adding cis-11-hexadecenal, cis-13-octadecenal, cis-9-hexadecenal and hexadecenal into a test tube to obtain the sex pheromone. Adding cis 9 trans 11-tetradecadiene acetate and cis 9 trans 12-tetradecadiene acetate into test tube to obtain two kinds of sex pheromone. Adding cis 7 cis 11 trans 13-hexadecadienal, cis 7 cis 11-hexadecadienal and cis 7-hexadecenoic acid into test tube to obtain three kinds of sex pheromones.
Adding a synergist accounting for 15 percent of the total weight of the second type of sex pheromone into the second type of sex pheromone, mixing 2 parts of the first type of sex pheromone, 1 part of the second type of sex pheromone and 2 parts of the third type of sex pheromone, and standing for 1 hour for later use.
(2) The antioxidant was added to the composition reagent, the light stabilizer was then added, and the mixture was stirred for 5min at a speed of 15r/min using a glass rod to obtain the final composition reagent.
Example 22
A compound insect sex pheromone for preventing and controlling rice field pests comprises 2 parts of first-class sex pheromone, 1 part of second-class sex pheromone and 2 parts of third-class sex pheromone, and the preparation method specifically comprises the following steps:
(1) adding cis-11-hexadecenal, cis-13-octadecenal, cis-9-hexadecenal and hexadecenal into a test tube to obtain the sex pheromone. Adding cis 9 trans 11-tetradecadiene acetate and cis 9 trans 12-tetradecadiene acetate into test tube to obtain two kinds of sex pheromone. Adding cis 7 cis 11 trans 13-hexadecadienal, cis 7 cis 11-hexadecadienal and cis 7-hexadecenoic acid into test tube to obtain three kinds of sex pheromones.
Adding a synergist accounting for 15 percent of the total weight of the second type of sex pheromone into the second type of sex pheromone, mixing 2 parts of the first type of sex pheromone, 1 part of the second type of sex pheromone and 2 parts of the third type of sex pheromone, and standing for 2 hours for later use.
(2) The antioxidant was added to the composition reagent, the light stabilizer was then added, and the mixture was stirred for 5min at a speed of 15r/min using a glass rod to obtain the final composition reagent.
Example 23
A compound insect sex pheromone for preventing and controlling rice field pests comprises 2 parts of first-class sex pheromone, 1 part of second-class sex pheromone and 2 parts of third-class sex pheromone, and the preparation method specifically comprises the following steps:
(1) adding cis-11-hexadecenal, cis-13-octadecenal, cis-9-hexadecenal and hexadecenal into a test tube to obtain the sex pheromone. Adding cis 9 trans 11-tetradecadiene acetate and cis 9 trans 12-tetradecadiene acetate into test tube to obtain two kinds of sex pheromone. Adding cis 7 cis 11 trans 13-hexadecadienal, cis 7 cis 11-hexadecadienal and cis 7-hexadecenoic acid into test tube to obtain three kinds of sex pheromones.
Adding a synergist accounting for 15 percent of the total weight of the second type of sex pheromone into the second type of sex pheromone, mixing 2 parts of the first type of sex pheromone, 1 part of the second type of sex pheromone and 2 parts of the third type of sex pheromone, and standing for 30min for later use.
(2) The antioxidant was added to the composition reagent, the light stabilizer was then added, and the mixture was stirred for 5min at a speed of 30r/min using a glass rod to obtain the final composition reagent.
Example 24
A compound insect sex pheromone for preventing and controlling rice field pests comprises 2 parts of first-class sex pheromone, 1 part of second-class sex pheromone and 2 parts of third-class sex pheromone, and the preparation method specifically comprises the following steps:
(1) adding cis-11-hexadecenal, cis-13-octadecenal, cis-9-hexadecenal and hexadecenal into a test tube to obtain the sex pheromone. Adding cis 9 trans 11-tetradecadiene acetate and cis 9 trans 12-tetradecadiene acetate into test tube to obtain two kinds of sex pheromone. Adding cis 7 cis 11 trans 13-hexadecadienal, cis 7 cis 11-hexadecadienal and cis 7-hexadecenoic acid into test tube to obtain three kinds of sex pheromones.
Adding a synergist accounting for 15 percent of the total weight of the second type of sex pheromone into the second type of sex pheromone, mixing 2 parts of the first type of sex pheromone, 1 part of the second type of sex pheromone and 2 parts of the third type of sex pheromone, and standing for 30min for later use.
(2) The antioxidant was added to the composition reagent, the light stabilizer was then added, and the mixture was stirred with a glass rod at a speed of 15r/min for 10min to obtain the final composition reagent.
TABLE 5 Components and weight ratios of reagents of compositions of examples 21-24
| Component/only | Example 2 | Example 3 | Example 4 | Example 5 | Example 6 | Example 7 |
| Cis 11-hexadecenal | 5 | 5 | 5 | 5 | 5 | 5 |
| Cis 13-octadecenal | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis-9-hexadecenal | 10 | 10 | 10 | 10 | 10 | 10 |
| Hexadecanal | 4 | 4 | 4 | 4 | 4 | 4 |
| Cis 9 trans 11-tetradecadieneacetic acid ester | 5 | 5 | 5 | 5 | 5 | 5 |
| Cis 9 trans 12-tetradecadieneacetic acid ester | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis 7 cis 11 trans 13-hexadecadienal | 3 | 3 | 3 | 3 | 3 | 3 |
| Cis 7 cis 11-hexadecadienal | 4 | 4 | 4 | 4 | 4 | 4 |
| Cis 7-hexadecenoic acid | 4 | 4 | 4 | 4 | 4 | 4 |
| Synergist | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 | 1.2 |
| Antioxidant agent | 3.28 | 3.28 | 3.28 | 3.28 | 3.28 | 3.28 |
| Light stabilizers | 2.46 | 2.46 | 2.46 | 2.46 | 2.46 | 2.46 |
Comparative example 1
A composite insect sex pheromone for preventing and controlling pests in paddy field is different from that in example 1 in that no synergist is added in the composition reagent.
Comparative example 2
A composite insect sex pheromone for preventing and controlling pests in paddy fields is different from that in example 1 in that an antioxidant is not added in a composition reagent.
Comparative example 3
A composite insect sex pheromone for preventing and controlling pests in paddy fields is different from that in example 1 in that no light stabilizer is added in the composition reagent.
Comparative example 4
A composite type insect sex pheromone for controlling rice field pests is different from that of example 1 in that the standing time of a composition reagent is less than 30 min.
Comparative example 5
A composite type insect sex pheromone for controlling rice field pests is different from example 23 in that the standing time of the composition agent exceeds 2 hours.
Comparative example 6
A composite type insect sex pheromone for controlling rice field pests is different from that of example 1 in that the stirring time of a composition reagent is less than 5 min.
Comparative example 7
A composite type insect sex pheromone for controlling rice field pests, which is different from example 25 in that the stirring time of the composition agent exceeds 10 min.
Capacity testing of agent Capture amounts of compositions
A composite type insect sex pheromone for controlling rice field pests prepared according to examples 1 to 25 and comparative examples 1 to 7 was tested for its ultimate practical effect after the components in the composition reagent and their relevant parameters were changed during the preparation process, depending on the number of male pests attracted by the composition reagent in practical use.
Selecting 10 mu of continuous rice fields with excellent rice variety, tensile strength and rich water conditions and consistent conditions as test fields, and respectively filling the compound insect sex pheromones of examples 1-25 and comparative examples 1-6 into traps for trapping tests; 5 points are randomly and uniformly arranged on each test sample, and the pest trapping quantity is counted every 2h for 6 times continuously. The test data are recorded in table 6.
TABLE 6 attracting effect of various kinds of sex pheromone change in composition agent on pest
| Chilo suppressalis trapping amount/rice stem borer trapping amount | Capture amount of Spodoptera litura | Capture amount of citrus leaf miner/fruit moth | |
| Example 1 | 11 | 6 | 10 |
| Example 2 | 13 | 7 | 9 |
| Example 3 | 19 | 11 | 14 |
| Example 4 | 24 | 6 | 11 |
| Example 5 | 17 | 5 | 10 |
| Example 6 | 16 | 3 | 8 |
| Example 7 | 14 | 9 | 9 |
| Example 8 | 15 | 16 | 8 |
| Example 9 | 15 | 17 | 11 |
| Example 10 | 14 | 17 | 7 |
| Example 11 | 14 | 14 | 9 |
| Example 12 | 13 | 5 | 11 |
| Example 13 | 14 | 6 | 9 |
| Example 14 | 14 | 7 | 15 |
| Example 15 | 16 | 9 | 17 |
| Example 16 | 15 | 10 | 17 |
| Example 17 | 13 | 5 | 13 |
| Example 18 | 13 | 5 | 11 |
| Example 19 | 21 | 17 | 16 |
| Example 20 | 18 | 8 | 19 |
| Example 21 | 19 | 16 | 17 |
| Example 22 | 15 | 15 | 14 |
| Example 23 | 16 | 13 | 12 |
| Example 24 | 9 | 7 | 9 |
| Comparative example 1 | 14 | 6 | 11 |
| Comparative example 2 | 3 | 4 | 3 |
| Comparative example 3 | 3 | 1 | 1 |
| Comparative example 4 | 14 | 13 | 12 |
| Comparative example 5 | 11 | 10 | 9 |
| Comparative example 6 | 9 | 6 | 7 |
As can be seen from the data in Table 6, in examples 1-6, with the increase of the hexadecanal part in the sex pheromone class, the trapping amount of the composition reagent to chilo suppressalis reaches the maximum at the middle value, and meanwhile, the composition reagent has the same effect on prodenia litura and citrus leaf miner, and the trapping effect of the composition reagent to pests is better. As the fraction of hexadecanal increases to a maximum, the number of traps by the composition agents for all three classes of male pests began to slip.
Examples 7 to 12, the number of the prodenia litura trapped by the composition reagent can be maximized at the median value with the increase of the number of cis 9 trans 11-tetradecadiene acetate in the second type of sex pheromone, and the change of the number of cis 9 trans 11-tetradecadiene acetate has little influence on the number of the striped rice borers trapped and has the effect on the citrus leaf miner equal to the number of the prodenia litura trapped. When the part of the cis-9 trans-11-tetradecadiene acetate reaches a high peak value, the composition reagent has a fallback effect on the trapping number of prodenia litura and citrus leaf miner, and has a small influence on the trapping number of chilo suppressalis.
Examples 13 to 18, with increasing cis 7 cis 11 hexadecadienal fraction in the three classes of sex pheromones,
the trapping amount of the composition reagent on the citrus leaf miner and the prodenia litura reaches the maximum at the median value, but the influence on the trapping amount of the chilo suppressalis is small. When the cis-7 cis-11-hexadecadienal fraction reaches a high peak value, the trapping amount of the composition reagent to the citrus leaf miner and the prodenia litura begins to have extremely obvious fall-back, and the trapping amount of the chilo suppressalis is also reduced to a certain degree.
Example 19, when the parts of the first type sex pheromone, the second type sex pheromone and the third type sex pheromone are the same, the trapping number of the composition agent to the three types of pests is all high, and at this time, the total trapping number of the composition agent to the three types of pests is the highest in the first 20 examples.
Example 20, when the addition ratio of the three types of sex pheromones is the highest, the trapping number of the composition reagent to the citrus leaf miner reaches the highest in the first 21 examples, but the trapping number of the composition reagent to the prodenia litura is greatly reduced, and the influence on the trapping number of the chilo suppressalis is small. Therefore, the trapping effect of the composition reagent on the three types of pests can be judged to be optimal when the input part ratio of the first type sex pheromone, the second type sex pheromone and the third type sex pheromone is 2:2: 2.
Examples 21 to 22, when the type one sex pheromone, the type two sex pheromone and the type three sex pheromone were mixed and left standing for 1 hour, the number of traps for the type three pests was all at a higher level, and they had a better trapping effect for pests than the agent obtained by leaving only the composition agent for 30 minutes. When the first type sex pheromone, the second type sex pheromone and the third type sex pheromone were mixed and left standing for 2 hours, the trapping amount of the composition for the three types of pests appeared to be slightly fallen back compared with that of example 22. Therefore, it was found that the composition reagent exhibited the optimum trapping effect when left standing for 1 hour.
In example 23, when the stirring speed of the composition reagent was increased to 30r/min, the number of the three types of pests trapped by the composition reagent dropped back to a large extent, and it was judged that the effect of trapping the three types of pests by the composition reagent was reduced by the stirring speed of the composition reagent that was too high, since the number of the three types of pests trapped by the composition reagent was reduced as compared with the stirring speed of 15 r/min.
Example 24, when the stirring time of the composition reagent reaches 10min, the composition reagent shows comprehensive fallback to the number of three types of pests, and the fallback data is obvious, so it can be determined that too long stirring time greatly reduces the effect of the composition reagent in trapping the three types of pests.
In comparative example 1, no synergist is added, so that the trapping amount of the composition agent to prodenia litura is greatly reduced, and the contrast with the common example is very obvious. Meanwhile, the trapping quantity of the chilo suppressalis and the citrus leaf miner also slightly slides down. Therefore, the addition of the synergist containing 15% of the second type of sex pheromone can improve the trapping quantity of the composition reagent on the three types of pests, particularly on prodenia litura.
Comparative examples 2 to 3 show a cliff-type drop in the number of traps for the three types of pests by the composition agent due to the reduction in the addition of the antioxidant and the light stabilizer, respectively, which greatly affects the number of traps for the three types of pests by the composition agent. Therefore, the antioxidant accounting for 8 percent of the total amount of the composition reagent and the light stabilizer accounting for 6 percent of the total amount of the composition reagent are added, so that the trapping effect of the composition reagent on the three types of pests can be effectively ensured and further improved.
Comparative example 4 the time for which the first type sex pheromone, the second type sex pheromone and the third type sex pheromone were mixed and left to stand was less than 30 minutes, which exhibited a certain degree of slip as compared with the number of traps of example 22, which was similar to that of example 23.
Comparative example 5 the time for mixing the first type sex pheromone, the second type sex pheromone and the third type sex pheromone and then standing for more than 2 hours also reduced the number of the three types of pests trapped by the composition agent, and the reduction range was larger than that of comparative example 4. Therefore, the composition reagent can be judged to be kept still for 1 hour to exert the trapping effect on the three types of pests to the maximum extent.
Comparative example 6 the stirring time of the composition agent was made less than 5min, which caused the occurrence of cliff-type slip in the number of traps of the composition agent for three types of pests, and the slip width was close to that of example 25, so it was judged that it is not preferable to make the stirring time too long or too short, and it is controlled between 5 and 10min, which maximized the effect of trapping the composition agent for three types of pests.
The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present invention.
Claims (10)
1. A compound insect sex pheromone for preventing and controlling pests in paddy fields is characterized in that a compound insect sex pheromone composition comprises a first type sex pheromone, a second type sex pheromone and a third type sex pheromone; the sex pheromone comprises the following components in parts by weight: 4-6 parts of cis-11-hexadecenal, 3-4 parts of cis-13-octadecenal, 5-18 parts of cis-9-hexadecenal and 1-6 parts of hexadecenal; the second type of sex pheromone comprises the following components in parts by weight: 3-8 parts of cis-9 trans-11-tetradecadiene acetate and 5-11 parts of cis-9 trans-12-tetradecadiene acetate; the three types of sex pheromones comprise the following components in parts by weight: 2-5 parts of cis 7 cis 11 trans 13-hexadecadienal, 3-8 parts of cis 7 cis 11-hexadecadienal and 4-9 parts of cis 7-hexadecenoic acid; the mass ratio of the first-class sex pheromone to the second-class sex pheromone to the third-class sex pheromone is (2-5): (1-2): (2-3).
2. The compound insect sex pheromone for preventing and controlling rice field pests as claimed in claim 1, wherein the second type sex pheromone further comprises a synergist, and the synergist is phenylacetaldehyde.
3. The compound type insect sex pheromone for controlling rice field pests as claimed in claim 2, wherein the synergist accounts for 15% of the total mass of the sex pheromones.
4. The compound insect sex pheromone for controlling rice field pests as claimed in claim 1, wherein the composition further comprises an antioxidant, and the antioxidant accounts for 8% of the total mass of the composition.
5. The compound insect sex pheromone for preventing and controlling rice field pests as claimed in claim 4, wherein the antioxidant is one or more of hindered phenols, phosphorous acids and thioesters.
6. The composite insect sex pheromone for controlling rice field pests as claimed in claim 4, wherein the composition further comprises a light stabilizer, and the light stabilizer accounts for 6% of the total mass of the composition.
7. The composite insect sex pheromone for paddy field pest control according to claim 6, wherein the light stabilizer is a hindered amine light stabilizer.
8. The process for producing a composite type insect sex pheromone for controlling rice field pests as claimed in any one of claims 1 to 7, which comprises the steps of: (1) preparing a composition reagent, mixing a synergist into a second-class sex pheromone solution under the condition of standard atmospheric pressure and 20-25 ℃, mixing the first-class sex pheromone, the second-class sex pheromone and the third-class sex pheromone according to a proportion, standing for later use; (2) and sequentially mixing the antioxidant and the light stabilizer in a certain proportion into the composition reagent, and uniformly mixing to obtain the compound insect sex pheromone reagent.
9. The method for producing a composite type insect sex pheromone for paddy field pest control according to claim 8, wherein in the step (1), the standing time of the composition agent is 30min to 2 hours.
10. The method for preparing a composite type insect sex pheromone for paddy field pest control according to claim 8, wherein in the step (2), the stirring frequency of the composition agent is 15 to 30r/min, and the stirring time is 5 to 10 min.
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| CN113016803A (en) * | 2021-03-16 | 2021-06-25 | 山西农业大学 | Sex pheromone attractant for elm spot moth |
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| CN101617652A (en) * | 2008-07-02 | 2010-01-06 | 宁波纽康生物技术有限公司 | Striped rice borer sex pheromone composition and inducement core thereof |
| CN101940208A (en) * | 2009-07-09 | 2011-01-12 | 慈溪市森林病虫防治检疫站 | Sex pheromone composite for luring prodenia litura and luring core thereof |
| CN102369925A (en) * | 2010-08-17 | 2012-03-14 | 宁波纽康生物技术有限公司 | Sex pheromone composition for luring citrus leaf miner and luring core thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101617652A (en) * | 2008-07-02 | 2010-01-06 | 宁波纽康生物技术有限公司 | Striped rice borer sex pheromone composition and inducement core thereof |
| CN101940208A (en) * | 2009-07-09 | 2011-01-12 | 慈溪市森林病虫防治检疫站 | Sex pheromone composite for luring prodenia litura and luring core thereof |
| CN102369925A (en) * | 2010-08-17 | 2012-03-14 | 宁波纽康生物技术有限公司 | Sex pheromone composition for luring citrus leaf miner and luring core thereof |
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| CN113016803A (en) * | 2021-03-16 | 2021-06-25 | 山西农业大学 | Sex pheromone attractant for elm spot moth |
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