CN111718872A - Microbial agent for crop blight and preparation method thereof - Google Patents
Microbial agent for crop blight and preparation method thereof Download PDFInfo
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- CN111718872A CN111718872A CN202010563401.3A CN202010563401A CN111718872A CN 111718872 A CN111718872 A CN 111718872A CN 202010563401 A CN202010563401 A CN 202010563401A CN 111718872 A CN111718872 A CN 111718872A
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 31
- 238000002360 preparation method Methods 0.000 title abstract description 12
- 241000315694 Bacillus tequilensis Species 0.000 claims abstract description 20
- 241000194105 Paenibacillus polymyxa Species 0.000 claims abstract description 20
- 241000223259 Trichoderma Species 0.000 claims abstract description 20
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 15
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 14
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 14
- 238000000855 fermentation Methods 0.000 claims abstract description 10
- 230000004151 fermentation Effects 0.000 claims abstract description 10
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 claims abstract description 6
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 claims abstract description 6
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 claims abstract description 6
- 240000008067 Cucumis sativus Species 0.000 claims abstract description 6
- 235000009849 Cucumis sativus Nutrition 0.000 claims abstract description 6
- 244000000626 Daucus carota Species 0.000 claims abstract description 6
- 235000002767 Daucus carota Nutrition 0.000 claims abstract description 6
- 240000005979 Hordeum vulgare Species 0.000 claims abstract description 6
- 235000007340 Hordeum vulgare Nutrition 0.000 claims abstract description 6
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims abstract description 6
- 240000007594 Oryza sativa Species 0.000 claims abstract description 6
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 6
- 240000003768 Solanum lycopersicum Species 0.000 claims abstract description 6
- 235000021307 Triticum Nutrition 0.000 claims abstract description 6
- 240000006677 Vicia faba Species 0.000 claims abstract description 6
- 235000010749 Vicia faba Nutrition 0.000 claims abstract description 6
- 235000002098 Vicia faba var. major Nutrition 0.000 claims abstract description 6
- 241000219094 Vitaceae Species 0.000 claims abstract description 6
- 235000021021 grapes Nutrition 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims abstract description 6
- 235000009566 rice Nutrition 0.000 claims abstract description 6
- 244000098338 Triticum aestivum Species 0.000 claims abstract 2
- 239000003795 chemical substances by application Substances 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 235000012015 potatoes Nutrition 0.000 claims description 4
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
- 239000002054 inoculum Substances 0.000 claims 2
- 230000002265 prevention Effects 0.000 abstract description 6
- 241000235342 Saccharomycetes Species 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 5
- HNJBEVLQSNELDL-UHFFFAOYSA-N pyrrolidin-2-one Chemical compound O=C1CCCN1 HNJBEVLQSNELDL-UHFFFAOYSA-N 0.000 abstract description 4
- 241001451061 Choanephora cucurbitarum Species 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 239000003814 drug Substances 0.000 description 9
- 201000010099 disease Diseases 0.000 description 8
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 8
- 241000209140 Triticum Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000013043 chemical agent Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 240000008790 Musa x paradisiaca Species 0.000 description 1
- 241000233679 Peronosporaceae Species 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000021015 bananas Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/32—Yeast
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
Abstract
The invention discloses a microbial agent for crop blight and a preparation method thereof, belonging to the technical field of agricultural control and prepared by fermenting the following raw materials, by weight, 20-30 parts of Bacillus tequilensis, 15-50 parts of trichoderma, 15-20 parts of Paenibacillus polymyxa, 500 parts of egg sugar 200-3‑1.5×10310-20 parts of yeast. According to the invention, through the synergistic fermentation of bacillus tequilensis, trichoderma, paenibacillus polymyxa, saccharomycetes, egg sugar and protein in a fermentation tank, a new substance pyrrolidone is generated in the process, and the novel pyrrolidone has broad-spectrum and efficient prevention and treatment effects on blight of potatoes, highland barley, broad beans, wheat, rice, cucumbers, grapes, Chinese cabbage, tomatoes and carrots, and is low in recurrence rate. In addition, the process is simple, and the cost of the microbial agent is effectively reduced.
Description
Technical Field
The invention belongs to the technical field of agricultural prevention and control, and particularly relates to a microbial agent for crop blight and a preparation method thereof.
Background
Blight (blight), also known as epidemic disease, is a disease of plants caused by fungi or bacteria, which is sudden, with symptoms including severe spotting, wilting or death of leaves, flowers, fruits, stems or whole plants, and tender tissue that grows rapidly is often attacked. Most important commercial crops are infected by one or more epidemic diseases. Epidemic diseases can affect flowers, leaves, bud bags, seedlings, twigs, stems and tips. The chemical agent is the most direct, effective and convenient method for preventing and treating plant diseases, but generally, the chemical agent is difficult to achieve the effect because pathogenic bacteria invade from the root and the stem base and then infect and spread in the vascular bundle. The developed microbial preparation for treating downy mildew has stronger specificity and can only aim at the blight of a specific crop; secondly, the control effect of the existing microbial preparations is not ideal.
Disclosure of Invention
The invention aims to: aiming at the problems, the invention provides a microbial agent for crop blight and a preparation method thereof.
The technical scheme adopted by the invention is as follows:
a microbial agent for crop blight is prepared by fermenting the following raw materials in parts by weightComprises 20 to 30 portions of Bacillus tequilensis, 15 to 50 portions of trichoderma, 15 to 20 portions of Paenibacillus polymyxa, 500 portions of egg sugar 200 and protein 1.0 × 103-1.5×10310-20 parts of yeast.
Preferably, the feed is prepared by fermenting the following raw materials, by weight, 25 parts of Bacillus tequilensis, 30 parts of trichoderma, 18 parts of paenibacillus polymyxa, 300 parts of egg sugar and 1.2 × 10 protein315 portions of yeast.
Preferably, the water-saving agent also comprises water 2.0 × 10 by weight4-2.5×104And (4) portions are obtained.
Preferably, the egg candy contains the following components in percentage by weight (3-5): (2-6) potato dregs and maltose.
Preferably, the Bacillus tequilensis contains not less than 3 × 10 per gram9cfu。
Preferably, the trichoderma contains not less than 2 × 10 per gram9cfu。
Preferably, the paenibacillus polymyxa contains not less than 4 × 10 per gram9cfu。
Preferably, the yeast contains not less than 5 × 10 per gram8cfu。
Preferably, the crops comprise potatoes, highland barley, broad beans, wheat, rice, cucumbers, grapes, Chinese cabbage, tomatoes and carrots.
A preparation method of a microbial agent for crop blight comprises the following steps of adding Bacillus tequilensis, trichoderma, paenibacillus polymyxa, saccharomycetes, egg sugar and protein into a fermentation tank, adding the above parts of water, and fermenting at 20-30 ℃ for 5 days to obtain the microbial agent for crop blight.
Compared with the prior art, the invention has the beneficial effects that:
according to the invention, through the synergistic fermentation of bacillus tequilensis, trichoderma, paenibacillus polymyxa, saccharomycetes, egg sugar and protein in a fermentation tank, a new substance pyrrolidone is generated in the process, and the novel pyrrolidone has broad-spectrum and efficient prevention and treatment effects on blight of potatoes, highland barley, broad beans, wheat, rice, cucumbers, grapes, Chinese cabbage, tomatoes and carrots, and is low in recurrence rate. In addition, the process is simple, and the cost of the microbial agent is effectively reduced.
Detailed Description
The present invention will be described in further detail in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the detailed description and specific examples, while indicating the preferred embodiment of the invention, are intended for purposes of illustration only and are not intended to limit the scope of the invention.
The microbial agent for crop blight and the preparation method thereof have broad spectrum, can be used for various crops, such as rice, beans, potatoes, highland barley, broad beans, wheat, bananas, cucumbers, grapes, Chinese cabbages, tomatoes and carrots, and is prepared by fermenting the following raw materials in parts by weight, 20-30 parts of Bacillus tequilensis, 15-50 parts of trichoderma, 15-20 parts of Paenibacillus polymyxa, 500 parts of egg sugar, 1.0 × 103-1.5×10310-20 parts of yeast, namely adding bacillus tequilensis, trichoderma, paenibacillus polymyxa, yeast, egg sugar and protein into a fermentation tank, adding the water in parts by weight, and fermenting for 5 days at the temperature of 20-30 ℃ to obtain the microbial agent for the crop blight, wherein each gram of the bacillus tequilensis contains not less than 3 × 109The trichoderma contains not less than 2 × 10 per gram9The paenibacillus polymyxa contains not less than 4 × 10 per gram9The yeast contains not less than 5 × 10 per gram8cfu。
Example 1
A microbial agent for crop blight is prepared by fermenting the following raw materials, by weight, 20 parts of Bacillus tequilensis, 15 parts of trichoderma, 15 parts of Paenibacillus polymyxa, 200 parts of egg sugar and 1.0 × 10 protein310 portions of yeast. The preparation method comprises the following steps of mixing Bacillus tequilensis, Trichoderma, Paenibacillus polymyxa, yeast and egg sugar,adding the protein into a fermentation tank, adding the water in the parts, and fermenting for 5 days at the temperature of 25 ℃ to obtain the microbial agent for the crop blight.
During prevention, the medicament of the embodiment is diluted and sprayed according to 400-fold liquid, and the incidence rate is counted after the medicament is taken once in 7-8 days; when the disease occurs, the medicament of the embodiment is sprayed according to the proportion of 150-; the results are detailed in table 1.
Example 2
A microbial agent for crop blight is prepared by fermenting the following raw materials, by weight, 25 parts of Bacillus tequilensis, 30 parts of trichoderma, 18 parts of Paenibacillus polymyxa, 300 parts of egg sugar and 1.2 × 10 protein315 portions of yeast. The preparation method comprises the following steps of adding bacillus tequilensis, trichoderma, paenibacillus polymyxa, saccharomycetes, egg sugar and protein into a fermentation tank, adding the water in parts, and fermenting for 5 days at the temperature of 25 ℃ to obtain the microbial agent for the crop blight.
During prevention, the medicament of the embodiment is diluted and sprayed according to 400-fold liquid, and the incidence rate is counted after the medicament is taken once in 7-8 days; when the disease occurs, the medicament of the embodiment is sprayed according to the proportion of 150-; the results are detailed in table 1.
Example 3
A microbial agent for crop blight is prepared by fermenting 30 parts by weight of Bacillus tequilensis, 50 parts by weight of trichoderma, 20 parts by weight of Paenibacillus polymyxa, 500 parts by weight of egg sugar and 1.5 × 10 parts by weight of protein320 portions of yeast. The preparation method comprises the following steps of adding bacillus tequilensis, trichoderma, paenibacillus polymyxa, saccharomycetes, egg sugar and protein into a fermentation tank, adding the water in parts, and fermenting for 5 days at the temperature of 25 ℃ to obtain the microbial agent for the crop blight.
During prevention, the medicament of the embodiment is diluted and sprayed according to 400-fold liquid, and the incidence rate is counted after the medicament is taken once in 7-8 days; when the disease occurs, the medicament of the embodiment is sprayed according to the proportion of 150-; the results are detailed in table 1.
Table 1 examples 1-3 statistics of experimental results for crop plants
As can be seen from table 1, the present invention has high control and treatment effects on potatoes, highland barley, broad beans, wheat, rice, cucumbers, grapes, Chinese cabbage, tomatoes, carrots, and has broad spectrum.
The above-mentioned embodiments only express the specific embodiments of the present application, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present application. It should be noted that, for those skilled in the art, without departing from the technical idea of the present application, several changes and modifications can be made, which are all within the protection scope of the present application.
Claims (10)
1. A microbial agent for crop blight is characterized by being prepared by fermenting the following raw materials, by weight, 20-30 parts of Bacillus tequilensis, 15-50 parts of trichoderma, 15-20 parts of Paenibacillus polymyxa, 500 parts of egg sugar, 1.0 × 103-1.5×10310-20 parts of yeast.
2. The microbial agent for the crop blight according to claim 1, which is prepared by fermenting the following raw materials, by weight, 25 parts of bacillus tequilensis, 30 parts of trichoderma, 18 parts of paenibacillus polymyxa, 300 parts of egg sugar, and 1.2 × 10 parts of protein315 portions of yeast.
3. The microbial agent for crop blight according to claim 1, further comprising water 2.0 × 10 parts by weight4-2.5×104And (4) portions are obtained.
4. The microbial agent for crop blight according to claim 1, wherein the egg sugar contains (3-5) by weight: (2-6) potato dregs and maltose.
5. The microbial agent for crop blight according to claim 1, wherein said Bacillus tequilensis contains not less than 3 × 10 per gram9cfu。
6. The microbial agent for crop blight according to claim 1, wherein said trichoderma contains not less than 2 × 10 per gram9cfu。
7. The microbial agent for crop blight according to claim 1, wherein said paenibacillus polymyxa contains not less than 4 × 10 per gram9cfu。
8. The microbial inoculant for crop blight according to claim 1, wherein said yeast comprises not less than 5 × 10/g8cfu。
9. The microbial inoculant for crop blight according to claim 1, wherein the crops comprise potatoes, highland barley, broad beans, wheat, rice, cucumbers, grapes, Chinese cabbage, tomatoes and carrots.
10. The method for preparing a microbial agent against crop blight according to any one of claims 1 to 9, comprising the steps of adding bacillus tequilensis, trichoderma, paenibacillus polymyxa, yeast, egg sugar, and protein into a fermentation tank, adding the above parts of water, and fermenting at 20-30 ℃ for 5 days to obtain the microbial agent against crop blight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010563401.3A CN111718872A (en) | 2020-06-19 | 2020-06-19 | Microbial agent for crop blight and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010563401.3A CN111718872A (en) | 2020-06-19 | 2020-06-19 | Microbial agent for crop blight and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111718872A true CN111718872A (en) | 2020-09-29 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010563401.3A Pending CN111718872A (en) | 2020-06-19 | 2020-06-19 | Microbial agent for crop blight and preparation method thereof |
Country Status (1)
| Country | Link |
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| CN (1) | CN111718872A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010825A (en) * | 2011-01-20 | 2011-04-13 | 上海创博生态工程有限公司 | Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof |
| CN102864081A (en) * | 2012-08-07 | 2013-01-09 | 上海交通大学 | Trichoderma strain for antagonizing cucumber fusarium wilt disease efficiently and application thereof |
| CN105695368A (en) * | 2016-04-11 | 2016-06-22 | 江苏省农业科学院 | Tequila bacillus and application thereof |
| CN105734000A (en) * | 2016-05-06 | 2016-07-06 | 南京农业大学 | Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases |
| CN109042742A (en) * | 2018-08-18 | 2018-12-21 | 杭州渔森农业技术开发有限公司 | Compound formulation is used to prevent and treat soil and passes the purposes fallen ill |
| CN111172084A (en) * | 2020-03-12 | 2020-05-19 | 河北省农林科学院植物保护研究所 | Bacillus tequilensis and application thereof |
-
2020
- 2020-06-19 CN CN202010563401.3A patent/CN111718872A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102010825A (en) * | 2011-01-20 | 2011-04-13 | 上海创博生态工程有限公司 | Microorganism compound bactericide for controlling cucumber fusarium wilt and preparation method thereof |
| CN102864081A (en) * | 2012-08-07 | 2013-01-09 | 上海交通大学 | Trichoderma strain for antagonizing cucumber fusarium wilt disease efficiently and application thereof |
| CN105695368A (en) * | 2016-04-11 | 2016-06-22 | 江苏省农业科学院 | Tequila bacillus and application thereof |
| CN105734000A (en) * | 2016-05-06 | 2016-07-06 | 南京农业大学 | Paenibacillus polymyxa NSY50 with capabilities of promoting growth and preventing diseases |
| CN109042742A (en) * | 2018-08-18 | 2018-12-21 | 杭州渔森农业技术开发有限公司 | Compound formulation is used to prevent and treat soil and passes the purposes fallen ill |
| CN111172084A (en) * | 2020-03-12 | 2020-05-19 | 河北省农林科学院植物保护研究所 | Bacillus tequilensis and application thereof |
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