CN111713399A - Method for producing hybrid rice combination by mixed sowing - Google Patents
Method for producing hybrid rice combination by mixed sowing Download PDFInfo
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- CN111713399A CN111713399A CN202010629590.XA CN202010629590A CN111713399A CN 111713399 A CN111713399 A CN 111713399A CN 202010629590 A CN202010629590 A CN 202010629590A CN 111713399 A CN111713399 A CN 111713399A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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Abstract
The invention discloses a method for producing hybrid rice combination by mixed sowing, in particular to the technical field of indica type hybrid rice seed production, which adopts a plurality of pretreatment modes, namely, sterile line seeds and restorer line seeds are synchronously or asynchronously treated according to the time difference from the sowing to the heading of the sterile line seeds and the restorer line seeds, and specifically comprises the following steps: A. when the time from sowing to the beginning of the spike is consistent, pretreating and accelerating germination; B. when the time from sowing to the beginning of the spike is different, asynchronous pretreatment and germination acceleration are carried out according to the time difference; C. after spraying 920 and pollinating, manually cutting off male parents by utilizing the plant height difference between the sterile line and the restorer line and the plant height difference caused by the different sensitivity of different lines of plants to gibberellic acid, and harvesting high-purity hybrid rice seeds after the male parents are mature. The method has strong operability and good social popularization and application, and compared with the traditional mode, the method improves the seed production efficiency and reduces the seed production cost.
Description
Technical Field
The invention relates to the technical field of indica type hybrid rice seed production, in particular to a method for producing hybrid rice combinations by mixed sowing.
Background
The generation of hybrid rice in 1981, the indica hybrid rice invented by Yuanlongpin et al has acquired the first national invention prize of prize et al in our country. The invention enables the rice breeding technology in China to jump the international leading position, creates great economic benefit and social benefit, and is used as the first agricultural technology in China to transfer the United states. The results obtained by the united nations textbook organization total stemmed bodham-carina were the second green revolution following international breeding of semi-dwarf rice in the 70 s. The parent of hybrid rice was named as Yuanlong Yuanlongping, by the International Rice institute and the former division of agriculture in India.
The male sterile line of rice is a special type of rice, and in the flower organ of the male sterile line, the male organ is not developed perfectly, normal pollen can not be formed, and the female organ is developed normally. Therefore, the rice cannot reproduce per se, and the seeds can be obtained by foreign rice pollen. The rice male sterile line is crossed with a rice male sterile maintainer line (the latter pollen is accepted), and the obtained seed next generation planting is still a sterile line. The rice male sterile line is crossed with a rice male sterile restoring line (the latter pollen is received), and the obtained next generation planting of the seeds is hybrid rice seeds in a general sense, namely seeds used by farmers in large-area production. The traditional method for obtaining hybrid rice seeds by hybridizing the male sterile line and the rice restorer line is too complex in operation, high in cost and difficult to popularize, and the method for producing the hybrid rice combination by mixed sowing utilizes the characteristic that hybrid rice male parents and female parents generally have height difference.
Disclosure of Invention
In order to overcome the defects in the prior art, the embodiment of the invention provides a method for producing hybrid rice combinations by mixed sowing, which forms a seed production technology of the mixed sowing hybrid rice capable of removing restorer lines by manually cutting off male parents through the plant height difference between a sterile line and the restorer line and the plant height difference caused by the different sensitivities of different lines of plants to gibberellic acid.
In order to achieve the purpose, the invention provides the following technical scheme: a method for producing hybrid rice combination by mixed sowing comprises the following steps:
step one, determining the seed quantity of parents: the seed consumption of the sterile line is 1.5kg per mu, the male parent (restorer line) is 1-2 grains per square, and 666-one grains per mu are 1332 grains;
step two: seed pretreatment: adopting various pretreatment modes, namely synchronously treating or asynchronously treating the sterile line seeds and the restorer line seeds according to the time difference between the sterile line seeds and the restorer line seeds from sowing to the initial spike, specifically comprising the following steps:
A. and (3) when the time from sowing to the beginning of the spike is consistent, simultaneously processing: simultaneously soaking seeds of parents and parents for accelerating germination;
B. when the time from sowing to the beginning of the spike is different, asynchronous processing is carried out according to the time difference:
the time difference is within one week: carrying out germination accelerating and seed soaking on the parent with long sowing initial period, and carrying out germination accelerating and seed soaking on the parent with short sowing initial period when the parent reaches the interval time;
if the difference is more than one week and less than 15 days, only the parent with long sowing beginning period is subjected to germination accelerating and seed soaking, and the other parent is not subjected to any treatment;
step three, mixed seeding: mixing the sterile line seeds and the restorer line seeds after germination acceleration according to the amount of pollen of the restorer line, and directly broadcasting the mixture in the field after uniform stirring;
step four, normal field management: carrying out weeding and fertilizer and water management according to standard field planting standards;
step five, pollination pretreatment: when the ear sprouting amount of the rice reaches 10 percent, spraying 20 percent gibberellic acid soluble powder with 5556-7142-time liquid, and spraying 15-30 g per mu;
step six, cutting off the male parent: after the pollination of the rice is finished, manually cutting off male parents according to the height difference between different parents;
step seven, harvesting: after the male parent is cut off, the remaining sterile line in the field is subjected to impurity removal management, and high-purity hybrid rice seeds can be harvested after the sterile line is mature.
In a preferred embodiment, in the second step, a plurality of pretreatment modes can be adopted, namely, the sterile line seeds and the restorer line seeds are synchronously or asynchronously treated according to the time difference between the sowing time and the initial ear time.
In a preferred embodiment, when the time from sowing to heading of the sterile line seeds and the time from sowing to heading of the restorer line seeds are consistent, the germination is pretreated simultaneously, and when the time from sowing to heading of the sterile line seeds and the time from sowing to heading of the restorer line seeds are different, the asynchronous pretreatment germination is carried out according to the time difference.
In a preferred embodiment, the time difference between sowing of the sterile line seed and the restorer line seed to the ear onset is less than 15 days.
In a preferred embodiment, in the third step, the seeding amount of the sterile line seeds is proportional to the pollen amount of the restorer line.
The invention has the technical effects and advantages that:
1. according to the hybrid-seeding hybrid rice seed production technology, the male parent and the female parent can be directly seeded by adopting the mode, the traditional transplanting planting is not needed, the process is simple, the equipment requirement is low, the operability is strong, the social popularization and application are good, the cost is greatly reduced compared with the traditional mode, and the benefit is improved;
2. the invention greatly reduces the seed consumption of the restorer line and can effectively reduce the seed production cost by uniformly and mixedly sowing the sterile line and the restorer line according to the layout mode of each square 1 restorer line
3. According to the invention, by uniformly sowing the male parent and the female parent in a mixed manner, the male parent is uniformly distributed, the pollen amount of the male parent is increased, the pollination obstacle is reduced, the seed production yield is greatly improved, manual pollen dispelling is not needed, the dependence on the amount of labor in the seed production process of hybrid rice is reduced, the seed production efficiency is improved, and the seed production cost is reduced.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
A method for producing hybrid rice combination by mixed sowing comprises the following steps:
step one, determining the seed quantity of parents: generally, the seed consumption of the sterile line is 1.5kg per mu, the seed consumption of the male parent (restorer line) is 1 seed per square, 666 seeds per mu, and the problem of emergence rate is sometimes worried that 2 seeds per square and 1332 seeds per mu are recommended;
step two: seed pretreatment: adopting various pretreatment modes, namely synchronously treating or asynchronously treating the sterile line seeds and the restorer line seeds according to the time difference between the sterile line seeds and the restorer line seeds from sowing to the initial spike, specifically comprising the following steps:
A. and (3) when the time from sowing to the beginning of the spike is consistent, simultaneously processing: simultaneously soaking seeds of parents and parents for accelerating germination;
B. when the time from sowing to the beginning of the spike is different, asynchronous processing is carried out according to the time difference:
the time difference is within one week: carrying out germination accelerating and seed soaking on the parent with long sowing initial period, and carrying out germination accelerating and seed soaking on the parent with short sowing initial period when the parent reaches the interval time;
if the difference is more than one week and less than 15 days, only the parent with long sowing beginning period is subjected to germination accelerating and seed soaking, and the other parent is not subjected to any treatment;
step three, mixed seeding: mixing the sterile line seeds and the restorer line seeds after germination acceleration according to the amount of pollen of the restorer line, and directly broadcasting the mixture in the field after uniform stirring;
step four, normal field management: carrying out weeding and fertilizer and water management according to standard field planting standards;
step five, pollination pretreatment: when the ear sprouting amount of the rice reaches 10 percent, spraying 20 percent gibberellic acid soluble powder with 5556-7142-time liquid, and spraying 15-30 g per mu;
step six, cutting off the male parent: after the pollination of the rice is finished, manually cutting off male parents according to the height difference between different parents;
step seven, harvesting: after the male parent is cut off, the remaining sterile line in the field is subjected to impurity removal management, and high-purity hybrid rice seeds can be harvested after the sterile line is mature.
Example 2
On the basis of the example 1, further, when the time from sowing to heading of the sterile line seeds is inconsistent with that of the restorer line seeds, asynchronous processing is carried out according to the time difference within one week: carrying out germination accelerating and seed soaking on the parent with long sowing initial period, and carrying out germination accelerating and seed soaking on the parent with short sowing initial period when the parent reaches the interval time;
mixing the sterile line seeds and the restorer line seeds after germination acceleration according to the amount of pollen of the restorer line, and directly broadcasting the mixture in the field after uniform stirring;
normal field management: carrying out weeding and fertilizer and water management according to standard field planting standards;
pre-treatment for pollination: when the ear sprouting amount of the rice reaches 10 percent, spraying 20 percent gibberellic acid soluble powder with 5556-7142-time liquid, and spraying 15-30 g per mu;
cutting off male parents: after the pollination of the rice is finished, manually cutting off male parents according to the height difference between different parents;
harvesting: after the male parent is cut off, the remaining sterile line in the field is subjected to impurity removal management, and high-purity hybrid rice seeds can be harvested after the sterile line is mature.
Example 3: need to be supplemented
On the basis of the embodiment 1-2, further, when the time from sowing to heading of the sterile line seeds is inconsistent with that of the restorer line seeds, asynchronous treatment is carried out according to the time difference, if the time difference is more than one week and less than 15 days, only the parent with long sowing beginning period is subjected to germination accelerating and seed soaking, and the other parent is not subjected to any treatment (seed soaking, germination and growth are directly completed in a field);
mixing the treated sterile line seeds and restorer line seeds according to the determined seed quantity proportion according to the pollen quantity of the restorer line, and directly broadcasting the sterile line seeds and the restorer line seeds in the field after uniformly stirring;
normal field management: carrying out weeding and fertilizer and water management according to standard field planting standards;
pre-treatment for pollination: when the ear sprouting amount of the rice reaches 10 percent, spraying 20 percent gibberellic acid soluble powder with 5556-7142-time liquid, and spraying 15-30 g per mu;
cutting off male parents: after the pollination of the rice is finished, manually cutting off male parents according to the height difference between different parents;
harvesting: after the male parent is cut off, the remaining sterile line in the field is subjected to impurity removal management, and high-purity hybrid rice seeds can be harvested after the sterile line is mature.
Example 4
On the basis of the above embodiments, further, in the second step, a plurality of pretreatment methods may be adopted, that is, the sterile line seeds and the restorer line seeds are synchronously or asynchronously treated according to the time difference between the sowing time and the heading time of the sterile line seeds and the restorer line seeds, specifically:
A. when the time from sowing to the beginning of the spike is consistent, pretreating and accelerating germination;
B. and when the time from sowing to the beginning of the spike is different, carrying out asynchronous pretreatment and germination acceleration according to the time difference.
Example 5
On the basis of the embodiment 3-4, further, when the time from sowing to heading of the sterile line seeds and the time from sowing to heading of the restorer line seeds are consistent, the sterile line seeds and the restorer line seeds are simultaneously treated, and the specific operations are as follows:
step one, determining the seed quantity of parents: generally, the seed consumption of the sterile line is 1.5kg per mu, the seed consumption of the male parent is 1 seed per square, and the seed consumption of the male parent is 666 seeds per mu (the sterile line: the restorer line); sometimes worrying about the problem of emergence rate will suggest 2 per square, 1332 per acre;
step two, when the time of sowing to the initial spike is consistent, preprocessing germination accelerating is carried out at the same time, and the parents are simultaneously soaked in seeds for germination accelerating;
step three, mixed seeding: mixing the sterile line seeds and the restorer line seeds after germination acceleration according to the amount of pollen of the restorer line, and directly broadcasting the mixture in the field after uniform stirring;
step four, normal field management: carrying out weeding and fertilizer and water management according to standard field planting standards;
step five, pollination pretreatment: when the ear sprouting amount of the rice reaches 10 percent, spraying 20 percent gibberellic acid soluble powder with 5556-7142-time liquid, and spraying 15-30 g per mu;
step six, cutting off the male parent: after the pollination of the rice is finished, manually cutting off male parents according to the height difference between different parents;
step seven, harvesting: after the male parent is cut off, the remaining sterile line in the field is subjected to impurity removal management, and high-purity hybrid rice seeds can be harvested after the sterile line is mature.
Example 6
On the basis of the embodiment 3-5, further, when the time from sowing to initial panicle of the sterile line seeds and the time from sowing to initial panicle of the restorer line seeds are inconsistent, performing asynchronous pretreatment pregermination according to the time difference from sowing to initial panicle of the sterile line seeds and the restorer line seeds, when the time from sowing to initial panicle of the sterile line seeds is greater than the time from sowing to initial panicle of the restorer line seeds, performing pretreatment pregermination on the sterile line seeds, then performing pretreatment pregermination on the restorer line seeds after corresponding days according to the numerical value of the time difference, and finally mixing and sowing simultaneously;
when the time from sowing to heading of the restorer line seeds is longer than that of the sterile line seeds, the restorer line seeds are pretreated for germination, then the sterile line seeds are pretreated for germination after corresponding days according to the numerical value of the time difference, and finally the restorer line seeds are mixed and sown at the same time.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments or portions thereof without departing from the spirit and scope of the invention.
Claims (5)
1. A method for producing hybrid rice combination by mixed seeding is characterized by comprising the following steps:
step one, determining the seed quantity of parents: the seed consumption of the sterile line is 1.5kg per mu, the male parent, namely the restorer line, is 1-2 grains per square, and 666-one 1332 grains per mu;
step two: seed pretreatment: adopting various pretreatment modes, namely synchronously treating or asynchronously treating the sterile line seeds and the restorer line seeds according to the time difference between the sterile line seeds and the restorer line seeds from sowing to the initial spike, specifically comprising the following steps:
A. and (3) when the time from sowing to the beginning of the spike is consistent, simultaneously processing: simultaneously soaking seeds of parents and parents for accelerating germination;
B. when the time from sowing to the beginning of the spike is different, asynchronous processing is carried out according to the time difference:
the time difference is within one week: carrying out germination accelerating and seed soaking on the parent with long sowing initial period, and carrying out germination accelerating and seed soaking on the parent with short sowing initial period when the parent reaches the interval time;
if the difference is more than one week and less than 15 days, only the parent with long sowing beginning period is subjected to germination accelerating and seed soaking, and the other parent is not subjected to any treatment;
step three, mixed seeding: mixing the sterile line seeds and the restorer line seeds after germination acceleration according to the amount of pollen of the restorer line, and directly broadcasting the mixture in the field after uniform stirring;
step four, normal field management: carrying out weeding and fertilizer and water management according to standard field planting standards;
step five, pollination pretreatment: when the ear sprouting amount of the rice reaches 10 percent, spraying 20 percent gibberellic acid soluble powder with 5556-7142-time liquid, and spraying 15-30 g per mu;
step six, cutting off the male parent: after the pollination of the rice is finished, manually cutting off male parents according to the height difference between different parents;
step seven, harvesting: after the male parent is cut off, the remaining sterile line in the field is subjected to impurity removal management, and high-purity hybrid rice seeds can be harvested after the sterile line is mature.
2. The method for producing hybrid rice combinations by mixed seeding according to claim 1, wherein the method comprises the following steps: in the first step, various pretreatment modes can be adopted, namely the sterile line seeds and the restorer line seeds are synchronously or asynchronously treated according to the time difference between the sowing of the sterile line seeds and the sowing of the restorer line seeds to the starting spike.
3. The method for producing hybrid rice combinations by mixed seeding according to claim 2, wherein the method comprises the following steps: and when the time from sowing to heading of the sterile line seeds and the time from sowing to heading of the restorer line seeds are consistent, simultaneously carrying out pretreatment and pregermination, and when the time from sowing to heading of the sterile line seeds and the time from sowing to heading of the restorer line seeds are different, carrying out asynchronous pretreatment and pregermination according to the time difference.
4. The method for producing hybrid rice combinations by mixed seeding according to claim 2, wherein the method comprises the following steps: the time difference between the sowing of the sterile line seeds and the restoring line seeds and the heading is less than 15 days.
5. The method for producing hybrid rice combinations by mixed seeding according to claim 1, wherein the method comprises the following steps: in the third step, the seeding amount of the sterile line seeds is in direct proportion to the pollen amount of the restorer line.
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Citations (4)
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| CN1176043A (en) * | 1997-08-08 | 1998-03-18 | 四川省万县市农业科学研究所 | Breeding technology for hybrid rice suitable for mechanization operation |
| CN103493726A (en) * | 2013-10-21 | 2014-01-08 | 安徽省农业科学院水稻研究所 | Method for mechanized seed production of hybrid rice through integration of seedling slinging technology and direct seeding technology |
| CN107646669A (en) * | 2017-09-12 | 2018-02-02 | 浙江科诚种业股份有限公司 | A kind of producing method for seed and breeding method of Medium Indica Hybrid Rice combination rice |
| CN109673506A (en) * | 2019-02-23 | 2019-04-26 | 湖南杂交水稻研究中心 | A kind of method of a large amount of preparing hybrid Rice Combinations |
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2020
- 2020-07-03 CN CN202010629590.XA patent/CN111713399A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1176043A (en) * | 1997-08-08 | 1998-03-18 | 四川省万县市农业科学研究所 | Breeding technology for hybrid rice suitable for mechanization operation |
| CN103493726A (en) * | 2013-10-21 | 2014-01-08 | 安徽省农业科学院水稻研究所 | Method for mechanized seed production of hybrid rice through integration of seedling slinging technology and direct seeding technology |
| CN107646669A (en) * | 2017-09-12 | 2018-02-02 | 浙江科诚种业股份有限公司 | A kind of producing method for seed and breeding method of Medium Indica Hybrid Rice combination rice |
| CN109673506A (en) * | 2019-02-23 | 2019-04-26 | 湖南杂交水稻研究中心 | A kind of method of a large amount of preparing hybrid Rice Combinations |
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Application publication date: 20200929 |