CN111700926A - Millettia speciosa champ extract, preparation method thereof and application thereof in medicines for reducing blood fat and losing weight - Google Patents
Millettia speciosa champ extract, preparation method thereof and application thereof in medicines for reducing blood fat and losing weight Download PDFInfo
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Abstract
The invention provides a beautiful millettia root extract, a preparation method thereof and application thereof in medicines for reducing blood fat and losing weight, belonging to the technical field of medical and health care products. The invention provides a preparation method of a beautiful millettia root extract, and the beautiful millettia root extract comprises a beautiful millettia root water extract, a beautiful millettia root alcohol extract, beautiful millettia root polysaccharide or beautiful millettia root polysaccharide filtrate. Research results show that the radix millettiae speciosae aqueous extract, the radix millettiae speciosae alcohol extract, the radix millettiae speciosae polysaccharide and the radix millettiae speciosae polysaccharide filtrate can inhibit the proliferation of 3T3-L1 precursor fat cells, can inhibit the conversion of 3T3-L1 precursor fat cells into fat cells, can reduce oil red O staining fat drops, has an inhibiting effect on the formation of the fat drops, and has an anti-obesity effect; in addition, the weight average of the body weight in 8 weeks of the volunteers is in a descending trend, the corresponding BMI index is in a descending trend and is in a normal range, and the results prove that the beautiful millettia root extract can be applied to preparation of medicines for reducing blood fat and/or losing weight.
Description
Technical Field
The invention belongs to the technical field of medical health-care products, and particularly relates to a beautiful millettia root extract, a preparation method thereof and application thereof in medicines for reducing blood fat and losing weight.
Background
Obesity and dyslipidemia are important risk factors for cardiovascular and cerebrovascular diseases. With the increase of living standard of people, the obesity and overweight people rapidly rise, the obesity becomes one of the great factors causing human death, and at least 280 ten thousand adults die every year in the world due to obesity-related diseases. Hyperlipidemia is one of the most typical complications of obesity, and 1200-1600 million people die of cardiovascular diseases caused by hyperlipidemia every year in the world. Therefore, the research and development of lipid-lowering and weight-losing medicines have great significance in both scientific and civil aspects, and the development potential is great. At present, weight-loss drugs such as sibutramine on the market have side effects such as abnormal liver function although they have some effects.
Radix Millettiae Speciosae is root of Millettia speciosa of Millettia of Leguminosae, and is named as radix Chimaphilae, radix Stephaniae Tetrandrae, rhizoma Nelumbinis, radix seu caulis Kadsurae Heteroclitae, and DALI. Produced in southern areas of China such as Fujian, Hunan, Guangdong, Guangxi, Hainan, Yunnan and Guizhou. The record of the materia medica records beautiful millettia root, strong bones and muscles, detoxication, internal injury regulation, traumatic injury treatment, wine infusion and kidney nourishing. The Chinese herbal medicine compilation records that the beautiful millettia root has sweet nature and flavor, is mild, tonifies deficiency and moistens lung, strengthens tendons and activates collaterals, and is used for lumbar muscle strain, rheumatoid arthritis, phthisis, chronic bronchitis, chronic hepatitis, spermatorrhea and leucorrhea. The Chinese plant record states that the beautiful millettia root has rich starch, can be used for brewing wine and can be used as a medicine, and has the functions of dredging the meridian passage, tonifying deficiency, moistening lung and strengthening spleen. Radix Millettiae Speciosae contains protein, polysaccharide, flavonoid, terpenoids, alkaloid, etc., and has effects of enhancing immunity, eliminating phlegm, relieving cough, resisting inflammation and relieving fatigue. The beautiful millettia root is commonly used for cooking soup, making medicated diet, medicinal liquor and the like in southern areas of China, such as Guangdong, Guangxi, Hainan and other areas, and has the remarkable effects of tonifying kidney and waist and strengthening tendons and bones. The health care and edible value of the beautiful millettia root is very high, and the beautiful millettia root is also a raw material medicine of Chinese patent medicines such as waist strengthening and kidney invigorating pills, strong body building capsules, and radix astragali tendon relaxing and collateral dredging tablets. However, the application of beautiful millettia root medicinal materials has not been developed completely, and the pharmacopoeia does not contain beautiful millettia root medicinal materials.
Disclosure of Invention
In view of the above, the present invention aims to provide a beautiful millettia root extract, a preparation method thereof and applications thereof in medicines for reducing blood fat and losing weight.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a preparation method of a beautiful millettia root extract, which comprises the following steps:
(1) 1g of beautiful millettia root and a solvent according to the mass-volume ratio of 1 g: mixing 9-11 ml of the raw materials, and soaking;
(2) extracting for 1-2 h, collecting the extracting solution, and filtering to obtain the liquid medicine containing the beautiful millettia root extract.
Preferably, when the solvent in the step (1) is water, the soaking time is 1-3 h, and the extraction in the step (2) is boiling extraction.
Preferably, when the solvent in the step (1) is ethanol water solution with the volume fraction of 70-80%, the soaking time is 10-14 h, and the extraction in the step (2) is heating reflux extraction at 90-110 ℃.
Preferably, when the solvent in the step (1) is water or an ethanol aqueous solution with a volume fraction of 70-80%, after the medicinal liquid containing the beautiful millettia root extract is obtained in the step (2), the method further comprises concentrating and drying the medicinal liquid containing the beautiful millettia root extract.
Preferably, the concentration is rotary evaporation concentration, the condensation temperature of the rotary evaporation concentration is-2 to 3 ℃, and the heating temperature is 35 to 60 ℃.
Preferably, the drying is vacuum drying, the vacuum degree of the vacuum drying is 0.1Mpa, the drying is carried out for 2 hours at 100 ℃, and then the drying is carried out for 3 hours at 80 ℃.
Preferably, when the solvent in the step (1) is water, after the medicinal liquid containing the beautiful millettia root extract is obtained in the step (2), the method further comprises the steps of mixing the medicinal liquid with absolute ethyl alcohol until the volume fraction of the absolute ethyl alcohol in the mixed liquid is 80-90%, standing for 46-50 h, and filtering to obtain filtrate and precipitate.
Further, vacuum drying the precipitate to obtain the beautiful millettia root extract which is beautiful millettia root polysaccharide; and concentrating and drying the filtrate to obtain the beautiful millettia root extract which is the beautiful millettia root polysaccharide filtrate.
The invention also provides the beautiful millettia root extract prepared by the preparation method.
The invention also provides application of the beautiful millettia root extract in preparing medicines for reducing blood fat and/or losing weight.
Compared with the prior art, the invention has the following beneficial effects:
the invention discovers for the first time that the beautiful millettia root extract comprises a beautiful millettia root water extract, beautiful millettia root polysaccharide, a beautiful millettia root polysaccharide filtrate and a beautiful millettia root alcohol extract, can inhibit the proliferation of precursor fat cells, inhibit the differentiation of fat cells and inhibit the formation of fat droplets, can reduce blood fat and lose weight, can be applied to blood fat reducing and/or weight losing medicines, provides scientific basis for reducing blood fat and losing weight of the beautiful millettia root, solves the blank of the beautiful millettia root in the field of reducing fat and losing weight, and expands the application range of the beautiful millettia root.
Drawings
FIG. 1 is a flow chart of the preparation of beautiful millettia root extract;
FIG. 2 is a graph of the inhibition of 3T3-L1 cell proliferation by an aqueous extract of Millettia speciosa champ;
FIG. 3 is a graph of Millettia speciosa polysaccharide inhibiting the proliferation of 3T3-L1 cells;
FIG. 4 is a graph of Millettia speciosa polysaccharide filtrate inhibiting proliferation of 3T3-L1 cells;
FIG. 5 is a graph showing that Millettia speciosa champ alcohol extract inhibits 3T3-L1 cell proliferation;
FIG. 6 is a graph of the inhibition of lipid drop formation by Millettia speciosa champ polysaccharide at different concentrations, wherein A is a blank control, B is a 0.1mg/mL Millettia speciosa aqueous extract, C is 0.1mg/mL Millettia speciosa polysaccharide, D is a 1mg/mL Millettia speciosa polysaccharide filtrate, and E is a 1mg/mL Millettia speciosa champ alcoholic extract;
FIG. 7 is a graph of the change in body weight of 12 volunteers over 8 weeks;
FIG. 8 is a graph of the trend of the BMI index change in 12 volunteers in 8 weeks.
Detailed Description
The invention provides a preparation method of a beautiful millettia root extract, which comprises the following steps:
(1) 1g of beautiful millettia root and a solvent according to the mass-volume ratio of 1 g: mixing 9-11 ml of the raw materials, and soaking;
(2) extracting for 1-2 h, collecting the extracting solution, and filtering to obtain the liquid medicine containing the beautiful millettia root extract.
The source of the beautiful millettia root is not specially limited, the beautiful millettia root is prepared from conventional commercial products, and beautiful millettia root decoction pieces are preferably adopted; in the present invention, preferably, the beautiful millettia root is pulverized and mixed with the solvent, the pulverized particle size is preferably 2mm, and the pulverized product is preferably sieved by a 10-mesh sieve; the pulverization is preferably carried out by dry pulverization by a pulverizer.
In the present invention, when the solvent in step (1) is preferably water, the mass-to-volume ratio of beautiful millettia root to water is 1 g: mixing 9-11 ml of the raw materials, and soaking; extracting for 1-2 h, collecting an extracting solution, and filtering to obtain a liquid medicine containing the beautiful millettia root aqueous extract; concentrating and drying to obtain the beautiful millettia root aqueous extract.
The invention has no special limitation on water, and can be realized by adopting water which is conventional in the field; the mixing mode of the beautiful millettia root and the water is not specially limited, and the conventional mixing mode in the field is adopted; in the present invention, the mass-to-volume ratio of beautiful millettia root to water is preferably 1 g: 10 ml. In the invention, the soaking time is preferably 1-3 h, more preferably 2h, and the soaking temperature is preferably 17-23 ℃, more preferably 20 ℃; in the present invention, the number of times of extraction is preferably 1 to 3 times, more preferably 2 times, and the preferred mode of each extraction is 230 to 270 ℃ heating and boiling, then 100 to 130 ℃ extraction, more preferably 250 ℃ heating and boiling, then 115 ℃ extraction, and the time for each extraction is preferably 1.5 hours. In the invention, the extracting solutions obtained by several times of extraction are combined and filtered; the filtering mode is preferably double-layer 200-mesh silk cloth filtering; in the invention, the concentration is preferably rotary evaporation concentration, the condensation temperature of the rotary evaporation concentration is preferably-2-3 ℃, the heating temperature is preferably 35-60 ℃, and the volume ratio of the concentrate obtained by the rotary evaporation concentration to the original solution is preferably 1: 10-20; in the invention, the drying is preferably vacuum drying, and the conditions of the vacuum drying are preferably 0.1Mpa, 100 ℃ for 2h, and 80 ℃ for 3 h.
In the invention, when the solvent in the step (1) is preferably water, the method further comprises the steps of mixing the liquid medicine containing the beautiful millettia root aqueous extract with absolute ethyl alcohol until the volume fraction of the absolute ethyl alcohol in the mixed liquid is 80-90%, standing for 46-50 h, and filtering to obtain filtrate and precipitate; vacuum drying the precipitate to obtain radix millettiae speciosae extract which is radix millettiae speciosae polysaccharide; and concentrating and drying the filtrate to obtain the beautiful millettia root extract which is the beautiful millettia root polysaccharide filtrate.
The mixing mode of the liquid medicine containing the beautiful millettia root aqueous extract and the absolute ethyl alcohol is not specially limited, and the conventional mixing mode in the field is adopted; the invention has no special limitation on the absolute ethyl alcohol, and the absolute ethyl alcohol is conventionally used in the field; in the present invention, the volume fraction of the anhydrous ethanol in the mixed solution is preferably 85%; in the invention, the standing time is preferably 48h, and the standing temperature is preferably 17-23 ℃, more preferably 20 ℃; in the invention, the concentration is preferably rotary evaporation concentration, the condensation temperature of the rotary evaporation concentration is preferably-2-3 ℃, the heating temperature is preferably 35-60 ℃, and the volume ratio of the concentrate obtained by the rotary evaporation concentration to the original solution is preferably 1: 15-17, more preferably 1: 16; in the invention, the drying is preferably vacuum drying, and the conditions of the vacuum drying are preferably 0.1Mpa, 100 ℃ for 2h, and 80 ℃ for 3 h.
In the invention, when the solvent in the step (1) is preferably ethanol water with a volume fraction of 70-80%, the mass-volume ratio of the beautiful millettia root to the ethanol water with a volume fraction of 70-80% is 1 g: mixing 9-11 ml of the raw materials, and soaking; extracting for 1-2 h, collecting an extracting solution, and filtering to obtain a liquid medicine containing the millettia speciosa champ alcohol extract; concentrating and drying to obtain the beautiful millettia root alcohol extract.
In the present invention, the solvent is more preferably an aqueous ethanol solution with a volume fraction of 75%; the mixing mode of the invention is not particularly limited, and the conventional mixing mode in the field is adopted; in the present invention, the mass-to-volume ratio of beautiful millettia root to the ethanol aqueous solution is preferably 1 g: 10 ml; in the invention, the soaking time is preferably 10-14 h, more preferably 12h, and the soaking temperature is preferably 17-23 ℃, more preferably 20 ℃; in the invention, the extraction mode is preferably heating reflux extraction, the heating reflux extraction temperature is preferably 90-110 ℃, more preferably 100 ℃, and the extraction time is preferably 1.5 h; in the invention, the filtering mode is preferably filtering by using double-layer 200-mesh silk cloth; in the invention, the concentration is preferably rotary evaporation concentration, the condensation temperature of the rotary evaporation concentration is preferably-2-3 ℃, the heating temperature is preferably 35-60 ℃, and the volume ratio of the concentrate obtained by the rotary evaporation concentration to the original solution is preferably 1: 15-17, more preferably 1: 16; in the invention, the drying is preferably vacuum drying, and the conditions of the vacuum drying are preferably 0.1Mpa, 100 ℃ for 2h, and 80 ℃ for 3 h.
The invention also provides the beautiful millettia root extract prepared by the preparation method, and the beautiful millettia root extract is preferably a beautiful millettia root water extract, a beautiful millettia root alcohol extract, beautiful millettia root polysaccharide or beautiful millettia root polysaccharide filtrate.
The invention also provides application of the beautiful millettia root extract in preparing medicines for reducing blood fat and/or losing weight. In the present invention, the hypolipidemic and/or anti-obesity drug preferably comprises the beautiful millettia root extract and a pharmaceutically acceptable carrier. In the invention, the mass percentage content of the beautiful millettia root extract in the medicine is preferably 10-25%.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
Preparation of beautiful millettia root aqueous extract
(1) 160g of beautiful millettia root is crushed and put into a 3L round-bottom flask, 1600ml of water is added, and the mixture is soaked for 2 hours at the temperature of 20 ℃.
(2) Placing the round-bottom flask in a heating jacket, heating to boil at 250 deg.C, adjusting to 115 deg.C, extracting for 1.5h, and collecting extractive solution; adding 1600ml of water, heating to boil at 250 deg.C, adjusting to 115 deg.C, heating and extracting for 1.5h, and collecting extractive solution; mixing the two extractive solutions, and filtering with double-layer 200-mesh silk cloth to obtain medicinal liquid containing radix Millettiae Speciosae water extract.
(3) Pouring the liquid medicine containing the beautiful millettia root water extract into a rotary evaporator, carrying out rotary evaporation and concentration to 100ml at a cold well of-2 ℃ and a water bath kettle of 50 ℃, collecting the concentrated solution, and carrying out vacuum drying on the concentrated solution: drying at 100 deg.C for 2 hr under 0.1Mpa, and drying at 80 deg.C for 3 hr to obtain dried radix Millettiae Speciosae water extract.
Example 2
Preparation of beautiful millettia root polysaccharide and beautiful millettia root polysaccharide filtrate
(1) 160g of beautiful millettia root is crushed and put into a 3L round-bottom flask, 1600ml of water is added, and the mixture is soaked for 2 hours at the temperature of 20 ℃.
(2) Placing the round-bottom flask in a heating jacket, heating to boil at 250 deg.C, adjusting to 115 deg.C, extracting for 1.5h, and collecting extractive solution; adding 1600ml of water, heating to boil at 250 deg.C, adjusting to 115 deg.C, heating and extracting for 1.5h, and collecting extractive solution; mixing the two extractive solutions, and filtering with double-layer 200-mesh silk cloth to obtain medicinal liquid containing radix Millettiae Speciosae water extract; adding absolute ethanol into the liquid medicine containing the beautiful millettia root aqueous extract until the volume fraction of the absolute ethanol in the mixed liquid is 85%, soaking for 48h at 20 ℃, and filtering by using double-layer 200-mesh silk cloth to obtain filtrate and precipitate.
(3) Drying the precipitate at 100 deg.C for 2 hr under 0.1Mpa, and drying at 80 deg.C for 3 hr to obtain dried radix Millettiae Speciosae polysaccharide;
pouring the filtrate into a rotary evaporator, cooling to-2 deg.C, heating in a water bath at 50 deg.C, concentrating to 100ml, collecting the concentrated solution, vacuum drying at 100 deg.C under 0.1Mpa for 2 hr, and drying at 80 deg.C for 3 hr to obtain dried radix Millettiae Speciosae polysaccharide filtrate.
Example 3
Preparation of beautiful millettia root alcohol extract
(1) 80g of beautiful millettia root is taken and crushed, and is put into a 1000ml round-bottom flask, 800ml of 75 percent ethanol solution is added, and the mixture is soaked for 12 hours at the temperature of 20 ℃.
(2) Placing 1000ml round flask in heating jacket, heating at 100 deg.C and reflux extracting for 1.5h, collecting extractive solution, and filtering with double-layer 200 mesh silk cloth to obtain medicinal liquid containing radix Millettiae Speciosae ethanol extract.
(3) Pouring the liquid medicine containing the beautiful millettia root alcohol extract into a rotary evaporator, carrying out rotary evaporation and concentration to 50ml in a cold well of-3 ℃ and a water bath kettle of 50 ℃, collecting concentrated solution, and carrying out vacuum drying on the concentrated solution: drying at 100 deg.C for 2 hr under 0.1Mpa, and drying at 80 deg.C for 3 hr to obtain dried radix Millettiae Speciosae ethanol extract.
Example 4
The extraction rates of the beautiful millettia root extracts prepared by the preparation methods described in examples 1 to 3 were calculated according to the following formulas, respectively, and the results are shown in table 1.
The extraction rate is the weight of the dried substance/the weight of the beautiful millettia root raw material
TABLE 1 beautiful Millettia speciosa champ sample extraction results
Example 5
Effect of Millettia speciosa samples on proliferation of precursor adipocytes
Complete medium (DMEM medium supplemented with 10% by volume fetal calf serum, 1% by volume penicillin and streptomycin) was used at 37 deg.C and 5% CO2Culturing 3T3-L1 precursor adipocytes in a saturated humidity incubator, and changing the culture solution every 2-3d for 1 time according to the cell growth state.
Taking 3T3-L1 precursor adipocytes in logarithmic growth phase, after trypsinization, suspending the culture medium into single cells, and diluting the cell concentration to 5 × 104And each/ml is inoculated to a cell proliferation detection culture plate special for RTCA, 100 mu L of cell suspension is added into each hole, after the cells adhere to the wall, culture media containing beautiful millettia root extracts with different concentrations (wherein the concentrations of the beautiful millettia root aqueous extracts are 0.25mg/ml and 0.5mg/ml respectively, the concentrations of the beautiful millettia root polysaccharides are 0.25mg/ml and 0.5mg/ml respectively, the concentrations of the beautiful millettia root polysaccharide filtrates are 0.25mg/ml, 0.5mg/ml and 1mg/ml respectively, and the concentrations of the beautiful millettia root alcohol extracts are 0.25mg/ml, 0.5mg/ml and 1mg/ml respectively) are added, a blank control group is arranged (the culture media do not contain beautiful millettia root extracts), real-time dynamic monitoring is carried out by adopting RTCA software, and instrument software observes a 3T3-L1 precursor adipocyte proliferation curve.
As can be seen from fig. 2, 3, 4 and 5, the beautiful millettia root aqueous extract, the beautiful millettia root polysaccharide filtrate and the beautiful millettia root alcohol extract at different concentrations all inhibited the proliferation of 3T3-L1 precursor adipocytes, and the inhibition ability was enhanced with the increase of the beautiful millettia root extract concentration compared with the blank control group.
Example 6
Inhibiting differentiation of precursor adipocytes into adipocytes
3T3-L1 cells in logarithmic growth phase described in example 5 were cultured at 1 × 105The density of each/ml was seeded in 96-well plates at 100. mu.l per well. After 48 hours, using culture media containing 0.5mmol/L IBMX, 1 mu mol/L dexamethasone, 10mg/L insulin and millettia speciosa champ extracts with different concentrations (0.1mg/ml millettia speciosa champ aqueous extract, 0.1mg/ml millettia speciosa champ polysaccharide, 1mg/ml millettia speciosa champ polysaccharide filtrate and 1mg/ml millettia speciosa champ ethanol extract) to induce and differentiate for 48 hours, then using the culture media containing 10mg/L insulin to culture for 48 hours, finally using complete culture media (10% by volume fetal calf serum and 1% by volume penicillin and streptomycin in total are added into the DMEM culture media to continue culture, changing the culture media 1 time every 2 days, and after culturing day 4, differentiating the cells into mature fat cells, using oil red O to carry out staining. Lipid droplet formation was observed under an inverted microscope and photographed.
As can be seen from fig. 6, compared with the blank control group, the beautiful millettia root aqueous extract, the beautiful millettia root polysaccharide filtrate and the beautiful millettia root alcohol extract can inhibit the 3T3-L1 precursor fat cells from being converted into fat cells, can reduce oil red O staining fat droplets, and have an inhibition effect on the formation of the fat droplets and an anti-obesity effect.
Example 7
Clinical data: selecting 12 volunteers with the age of 20-60 years, and not taking similar health products or medicines.
The treatment method comprises the following steps: the beautiful millettia root polysaccharide prepared in the embodiment 2 of the invention is taken after being mixed with boiled water and meals, 2.5g is taken twice a day, 4 weeks are taken for 1 treatment course.
Observation indexes are as follows: body weight.
During the test period, all volunteers live according to the respective past living habits such as diet, work and rest, exercise and the like, and the volunteers are weighed and recorded at the beginning of the test; thereafter, the body weight was weighed at the same time every week by the same scale, recorded for eight weeks, and the results were subjected to statistical analysis.
As can be seen from fig. 7 and 8, the weight average of the subjects in 8 weeks showed a downward trend, and the corresponding BMI index also showed a downward trend, and most of the subjects gradually showed a normal range, while the 12# volunteers failed to decrease to a normal level after 8 weeks due to the excessive initial weight and initial BMI index, but the weight gradually decreased and the BMI gradually decreased in the whole 8 weeks.
In addition, during the period of taking the beautiful millettia root polysaccharide, symptoms such as malaise, allergy and the like do not appear in each volunteer.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The preparation method of the beautiful millettia root extract is characterized by comprising the following steps:
(1) 1g of beautiful millettia root and a solvent according to the mass-volume ratio of 1 g: mixing 9-11 ml of the raw materials, and soaking;
(2) extracting for 1-2 h, collecting the extracting solution, and filtering to obtain the liquid medicine containing the beautiful millettia root extract.
2. The preparation method according to claim 1, wherein when the solvent in the step (1) is water, the soaking time is 1-3 h, and the extraction in the step (2) is boiling extraction.
3. The preparation method according to claim 1, wherein when the solvent in step (1) is 70-80% ethanol water by volume, the soaking time is 10-14 h, and the extraction in step (2) is heating reflux extraction at 90-110 ℃.
4. The preparation method according to any one of claims 2 or 3, wherein the step (2) of obtaining the radix Millettiae Speciosae extract-containing liquid medicine further comprises concentrating and drying the radix Millettiae Speciosae extract-containing liquid medicine.
5. The preparation method according to claim 4, wherein the concentration is rotary evaporation concentration, the condensation temperature of the rotary evaporation concentration is-2 to 3 ℃, and the heating temperature is 35 to 60 ℃.
6. The method according to claim 4, wherein the drying is carried out under vacuum at a vacuum of 0.1MPa at 100 ℃ for 2 hours and then at 80 ℃ for 3 hours.
7. The preparation method of the millettia speciosa champ extract as claimed in claim 2, wherein the step (2) of obtaining the liquid medicine containing the millettia speciosa champ extract further comprises the steps of mixing the liquid medicine with absolute ethyl alcohol until the volume fraction of the absolute ethyl alcohol in the mixed liquid is 80-90%, standing for 46-50 h, and filtering to obtain filtrate and precipitate.
8. The method for preparing the beautiful millettia root extract according to claim 7, characterized in that the precipitate is vacuum-dried to obtain beautiful millettia root extract which is beautiful millettia root polysaccharide; and concentrating and drying the filtrate to obtain the beautiful millettia root extract which is the beautiful millettia root polysaccharide filtrate.
9. The Millettia speciosa champ extract prepared by the preparation method according to any one of claims 1 to 8.
10. Use of the millettia speciosa champ extract of claim 9 for the preparation of a medicament for lowering blood lipids and/or weight loss.
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| CN112451558A (en) * | 2020-11-18 | 2021-03-09 | 广东药科大学 | Application of beautiful millettia root alcohol extract in preparing hypoglycemic or hypolipemic drugs and health products |
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