CN111700152A - Antibiotic-free feed prepared by microbial fermentation and preparation process thereof - Google Patents
Antibiotic-free feed prepared by microbial fermentation and preparation process thereof Download PDFInfo
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
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- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
- A23K10/38—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/00—Feeding-stuffs specially adapted for particular animals
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
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- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
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Abstract
The invention discloses a nonreactive feed prepared by microbial fermentation, which comprises the following raw materials in parts by weight: 30-50 parts of crop straw, 10-15 parts of rice hull, 10-15 parts of corn cob, 20-30 parts of vinasse, 10-15 parts of bean pulp, 10-15 parts of fish meal, 10-15 parts of wheat bran, 3-5 parts of zymogen liquid and 2-3 parts of composite zymogen liquid, and the preparation process comprises the following steps: 1) respectively crushing and mixing crop straws, rice husks, corn cobs, vinasse and wheat bran, adding water and zymogen liquid, carrying out anaerobic fermentation, and carrying out heating sterilization treatment after fermentation; 2) crushing bean pulp, mixing with fish meal, dissolving in water, adding composite zymogen liquid, and fermenting; 3) pouring the fermentation liquor obtained in the step 2) into the raw materials obtained in the step 1), continuing anaerobic fermentation, drying at 60 ℃ after fermentation, and granulating. The feed of the invention improves the resistance of animals, and has the effects of preventing and treating digestive tract diseases and promoting growth.
Description
Technical Field
The invention relates to the technical field of fermented feed, in particular to antibiotic-free feed prepared by microbial fermentation and a preparation process thereof.
Background
The addition of antibiotics in the feed plays a positive role in preventing animal diseases, promoting animal growth, improving feed return, improving the yield of livestock and poultry products and the like. With the wide application of antibiotics as feed additives, various disadvantages of antibiotics are known, such as causing the normal flora in the gastrointestinal tract of animals to be disordered and generating drug resistance. The long-term use of antibiotics results in a large amount of residues in meat and egg products in animal production, and the residues have toxicity, teratogenicity, carcinogenicity and mutagenicity to human bodies. The probiotic preparation has the advantages of no toxic or side effect, no drug resistance, no residue, remarkable effect, low cost and the like, can effectively supplement beneficial microorganisms in the digestive tract, kill harmful microorganisms, particularly pathogenic bacteria such as escherichia coli and the like, improve the flora balance of the digestive tract, and rapidly improve the disease resistance, metabolism and feed absorption capacity of the organism, thereby achieving the double effects of preventing and treating digestive tract diseases and promoting growth.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the technical problems in the related art. Therefore, the invention aims to provide a non-antibiotic feed prepared by microbial fermentation and a preparation process thereof, wherein a high-fiber raw material is subjected to anaerobic fermentation to decompose fiber, so that the raw material is more beneficial to the absorption of animals, and the raw material with high protein content is used for beneficial bacteria fermentation to prepare the non-antibiotic beneficial bacteria feed.
The antibiotic-free feed prepared by microbial fermentation provided by the invention comprises the following raw materials in parts by weight: 30-50 parts of crop straws, 10-15 parts of rice husks, 10-15 parts of corn cobs, 20-30 parts of vinasse, 10-15 parts of bean pulp, 10-15 parts of fish meal, 10-15 parts of wheat bran, 3-5 parts of zymogen liquid and 2-3 parts of composite zymogen liquid, wherein the zymogen liquid is a mixed bacterium of bacillus subtilis, candida utilis and aspergillus niger, and the composite zymogen liquid is a mixed bacterium of lactobacillus bulgaricus, lactobacillus acidophilus, saccharomycetes and aspergillus oryzae.
In some embodiments of the invention, the crop straw comprises corn straw, rice straw, and soybean straw.
In other embodiments of the present invention, the fish meal is full-fat fish meal, and the fish meal is obtained by drying, crushing and pulverizing fresh fish with a fresh weight of not more than 30 g.
In other embodiments of the present invention, the concentration of each bacterium in the fermented liquid is 1 × 108One per ml.
In other embodiments of the present invention, the concentration of each bacterium in the composite zymophyte liquid is 1 × 107One per ml.
A preparation process of antibiotic-free feed prepared by microbial fermentation comprises the following specific process steps:
1) respectively crushing and mixing crop straws, rice husks, corn cobs, vinasse and wheat husks, adding water, stirring and dissolving, adding zymogen liquid, carrying out anaerobic fermentation for 3-5 days, and carrying out heating sterilization treatment after fermentation is finished, wherein the heating temperature is 75-80 ℃, and the heating time is 25-30 minutes;
2) pulverizing soybean meal, mixing with fish meal, dissolving in water, stirring, adding compound zymocyte liquid, fermenting at 40-45 deg.C for 5-7 hr;
3) pouring the fermentation liquor obtained in the step 2) into the raw materials obtained in the step 1), continuing anaerobic fermentation for 3-5 hours, and after the fermentation is finished, drying at 60 ℃ and granulating.
In other embodiments of the present invention, the fermented bacterial liquid is a mixture of bacillus subtilis, candida utilis and aspergillus niger, and the composite fermented bacterial liquid is a mixture of lactobacillus bulgaricus, lactobacillus acidophilus, yeast and aspergillus oryzae.
In the invention, the high-fiber raw material is subjected to anaerobic fermentation to decompose the fiber to generate amino acid substances, so that the absorption by animals is facilitated, and the animal resistance is improved. Then, the raw materials with high protein content are used for fermenting beneficial bacteria to generate high-concentration beneficial bacteria, and meanwhile, the protein is refined, so that the absorption of animals is facilitated, and the animal immunity is improved. Finally, the fiber raw material after anaerobic fermentation is continuously fermented by probiotics, and the antibiotic-free feed is prepared by drying and granulating, and has double effects of preventing and treating digestive tract diseases and promoting growth.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
Example 1
The antimicrobial-free feed prepared by microbial fermentation comprises, by weight, 30-50 parts of crop straws, 10-15 parts of rice hulls, 10-15 parts of corn cobs, 20-30 parts of vinasse, 10-15 parts of soybean meal, 10-15 parts of fish meal, 10-15 parts of wheat bran, 3-5 parts of zymogen liquid and 2-3 parts of composite zymogen liquid, wherein the zymogen liquid is a mixed bacterium of bacillus subtilis, candida utilis and aspergillus niger, the composite zymogen liquid is a mixed bacterium of lactobacillus bulgaricus, lactobacillus acidophilus, saccharomycetes and aspergillus oryzae, the crop straws comprise corn straws, rice straws and soybean straws, and the concentration of each bacterium in the zymogen liquid is 1 × 108Each bacterium in the composite zymophyte liquid has the concentration of 1 × 107One per ml.
A preparation process of antibiotic-free feed prepared by microbial fermentation comprises the following specific process steps:
1) respectively crushing and mixing crop straws, rice husks, corn cobs, vinasse and wheat husks, adding water, stirring and dissolving, adding zymogen liquid, carrying out anaerobic fermentation for 3-5 days, and carrying out heating sterilization treatment after fermentation is finished, wherein the heating temperature is 75-80 ℃, and the heating time is 25-30 minutes;
2) pulverizing soybean meal, mixing with fish meal, dissolving in water, stirring, adding compound zymocyte liquid, fermenting at 40-45 deg.C for 5-7 hr;
3) pouring the fermentation liquor obtained in the step 2) into the raw materials obtained in the step 1), continuing anaerobic fermentation for 3-5 hours, and after the fermentation is finished, drying at 60 ℃ and granulating.
Example 2
The antimicrobial-free feed prepared by microbial fermentation comprises, by weight, 30-50 parts of crop straws, 10-15 parts of rice hulls, 10-15 parts of corn cobs, 20-30 parts of vinasse, 10-15 parts of soybean meal, 10-15 parts of fish meal, 10-15 parts of wheat bran, 3-5 parts of zymogen liquid and 2-3 parts of composite zymogen liquid, wherein the zymogen liquid is a mixed bacterium of bacillus subtilis, candida utilis and aspergillus niger, the composite zymogen liquid is a mixed bacterium of lactobacillus bulgaricus, lactobacillus acidophilus, saccharomycetes and aspergillus oryzae, the crop straws comprise corn straws, rice straws and soybean straws, and the concentration of each bacterium in the zymogen liquid is 1 × 108Each bacterium in the composite zymophyte liquid has the concentration of 1 × 107One per ml.
The preparation process of a non-antibiotic feed prepared by microbial fermentation is the same as that of example 1. The differences are as follows: step 1), anaerobic fermentation is carried out for 3-5 days, the heating sterilization temperature is 75-80 ℃, and the heating is carried out for 25-30 minutes; 2. step 2), fermenting for 5-7 hours at the fermentation temperature of 40-45 ℃; 3. and 3) anaerobic fermentation is carried out for 3-5 hours.
Example 3
The antimicrobial-free feed prepared by microbial fermentation comprises, by weight, 30-50 parts of crop straws, 10-15 parts of rice hulls, 10-15 parts of corn cobs, 20-30 parts of vinasse, 10-15 parts of soybean meal, 10-15 parts of fish meal, 10-15 parts of wheat bran, 3-5 parts of zymogen liquid and 2-3 parts of composite zymogen liquid, wherein the zymogen liquid is a mixed bacterium of bacillus subtilis, candida utilis and aspergillus niger, the composite zymogen liquid is a mixed bacterium of lactobacillus bulgaricus, lactobacillus acidophilus, saccharomycetes and aspergillus oryzae, the crop straws comprise corn straws, rice straws and soybean straws, and the concentration of each bacterium in the zymogen liquid is 1 × 108Each bacterium in the composite zymophyte liquid has the concentration of 1 × 107One per ml.
The preparation process of a non-antibiotic feed prepared by microbial fermentation is the same as that of example 1. The differences are as follows: step 1), anaerobic fermentation is carried out for 3-5 days, the heating sterilization temperature is 75-80 ℃, and the heating is carried out for 25-30 minutes; 2. step 2), fermenting for 5-7 hours at the fermentation temperature of 40-45 ℃; 3. and 3) anaerobic fermentation is carried out for 3-5 hours.
Example 4
The antimicrobial-free feed prepared by microbial fermentation comprises, by weight, 30-50 parts of crop straws, 10-15 parts of rice hulls, 10-15 parts of corn cobs, 20-30 parts of vinasse, 10-15 parts of soybean meal, 10-15 parts of fish meal, 10-15 parts of wheat bran, 3-5 parts of zymogen liquid and 2-3 parts of composite zymogen liquid, wherein the zymogen liquid is a mixed bacterium of bacillus subtilis, candida utilis and aspergillus niger, the composite zymogen liquid is a mixed bacterium of lactobacillus bulgaricus, lactobacillus acidophilus, saccharomycetes and aspergillus oryzae, the crop straws comprise corn straws, rice straws and soybean straws, and the concentration of each bacterium in the zymogen liquid is 1 × 108Each bacterium in the composite zymophyte liquid has the concentration of 1 × 107One per ml.
The preparation process of a non-antibiotic feed prepared by microbial fermentation is the same as that of example 1. The differences are as follows: step 1), anaerobic fermentation is carried out for 3-5 days, the heating sterilization temperature is 75-80 ℃, and the heating is carried out for 25-30 minutes; 2. step 2), fermenting for 5-7 hours at the fermentation temperature of 40-45 ℃; 3. and 3) anaerobic fermentation is carried out for 3-5 hours.
Example 5
The preparation process of the feed prepared by fermenting all the raw materials with zymophyte liquid, namely the microbial fermentation, is the same as that in example 1, but does not have the fermentation of the compound zymophyte liquid, and comprises the following specific process steps:
1) respectively crushing and mixing crop straws, rice hulls, corn cobs, vinasse and wheat bran, adding water, stirring and dissolving, crushing soybean meal, mixing with fish meal, adding the mixture into the raw materials, adding zymogen liquid, carrying out anaerobic fermentation for 3-5 days, and carrying out heating sterilization treatment after the fermentation is finished, wherein the heating temperature is 75-80 ℃, and the heating time is 25-30 minutes;
2) drying the mixture at 60 ℃ after the fermentation in the step 1) is finished, and granulating.
Example 6
The raw materials are added with antibiotics before being dried before the step 2) as in the example 5, and then are dried and granulated.
The 100 chickens were fed with the feed prepared in examples 1 to 6, and the growth conditions of 100 days, including the death condition during the growth process, the number of sick chickens during the growth process, and the weight of the chickens after 100 days of growth, were observed.
Table 1 examples 1-6 feed conditions after 100 days of feeding 100 chickens each
The increase in body weight of the chickens fed with the feed of examples 1-4 was significantly higher than that of examples 4 and 5, while the mortality rate was low and the number of sick chickens was also significantly lower than that of examples 4 and 5.
The feed prepared in examples 1-6 was used to feed 100 ducks and the growth of 100 days including the fatality of the ducks in the growth process, the number of the ducks with diseases in the growth process and the weight of the ducks after 100 days of growth were observed.
TABLE 2 examples 1-6 feed conditions after 100 days of feeding 100 ducks
The body weight increase of the ducks fed by the feed of the examples 1-4 is obviously higher than that of the ducks fed by the examples 4 and 5, the fatality rate is low, and the number of sick chickens is also obviously lower than that of the ducks fed by the feeds of the examples 4 and 5.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. A antibiotic-free feed prepared by microbial fermentation is characterized in that: the feed comprises the following raw materials in parts by weight: 30-50 parts of crop straws, 10-15 parts of rice husks, 10-15 parts of corn cobs, 20-30 parts of vinasse, 10-15 parts of bean pulp, 10-15 parts of fish meal, 10-15 parts of wheat bran, 3-5 parts of zymogen liquid and 2-3 parts of composite zymogen liquid, wherein the zymogen liquid is a mixed bacterium of bacillus subtilis, candida utilis and aspergillus niger, and the composite zymogen liquid is a mixed bacterium of lactobacillus bulgaricus, lactobacillus acidophilus, saccharomycetes and aspergillus oryzae.
2. The antimicrobial-free feed prepared by microbial fermentation according to claim 1, wherein: the crop straws comprise corn straws, rice straws and soybean straws.
3. The antimicrobial-free feed prepared by microbial fermentation according to claim 1, wherein: the fish meal is full-fat fish meal, and is prepared by drying, crushing and pulverizing fresh fish with the weight not more than 30 g.
4. The antimicrobial-free feed prepared by microbial fermentation according to claim 1, wherein the concentration of each bacterium in the fermented solution is 1 × 108One per ml.
5. The antimicrobial-free feed prepared by microbial fermentation according to claim 1, wherein the concentration of each bacterium in the composite zymophyte liquid is 1 × 107One per ml.
6. A preparation process of antibiotic-free feed prepared by microbial fermentation is characterized by comprising the following steps: the specific process steps are as follows:
1) respectively crushing and mixing crop straws, rice husks, corn cobs, vinasse and wheat husks, adding water, stirring and dissolving, adding zymogen liquid, carrying out anaerobic fermentation for 3-5 days, and carrying out heating sterilization treatment after fermentation is finished, wherein the heating temperature is 75-80 ℃, and the heating time is 25-30 minutes;
2) pulverizing soybean meal, mixing with fish meal, dissolving in water, stirring, adding compound zymocyte liquid, fermenting at 40-45 deg.C for 5-7 hr;
3) pouring the fermentation liquor obtained in the step 2) into the raw materials obtained in the step 1), continuing anaerobic fermentation for 3-5 hours, and after the fermentation is finished, drying at 60 ℃ and granulating.
7. The process according to claim 6, wherein the antimicrobial-free feed is prepared by fermenting the microorganism, and the process comprises the following steps: the zymocyte liquid is a mixed bacterium of bacillus subtilis, candida utilis and aspergillus niger, and the composite zymocyte liquid is a mixed bacterium of lactobacillus bulgaricus, lactobacillus acidophilus, saccharomycetes and aspergillus oryzae.
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| WO2022012160A1 (en) * | 2020-07-15 | 2022-01-20 | 安徽省正大源饲料集团有限公司 | Sucking pig feed prepared by microbial fermentation and preparation method therefor |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461653A (en) * | 2013-09-26 | 2013-12-25 | 卞佳林 | Yeast biological forage and preparation method thereof |
| CN107410698A (en) * | 2017-09-04 | 2017-12-01 | 重庆晋豪美耐皿制品有限公司 | Straw feed composition and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103461653A (en) * | 2013-09-26 | 2013-12-25 | 卞佳林 | Yeast biological forage and preparation method thereof |
| CN107410698A (en) * | 2017-09-04 | 2017-12-01 | 重庆晋豪美耐皿制品有限公司 | Straw feed composition and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2022012160A1 (en) * | 2020-07-15 | 2022-01-20 | 安徽省正大源饲料集团有限公司 | Sucking pig feed prepared by microbial fermentation and preparation method therefor |
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