CN111700012A - Method for improving survival rate of procambarus clarkii cultured in laboratory - Google Patents

Method for improving survival rate of procambarus clarkii cultured in laboratory Download PDF

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Publication number
CN111700012A
CN111700012A CN202010568723.7A CN202010568723A CN111700012A CN 111700012 A CN111700012 A CN 111700012A CN 202010568723 A CN202010568723 A CN 202010568723A CN 111700012 A CN111700012 A CN 111700012A
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culture
water
cage
box
reservoir
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白旭峰
彭波
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Huazhong Agricultural University
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Huazhong Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/59Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/003Aquaria; Terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/003Aquaria; Terraria
    • A01K63/006Accessories for aquaria or terraria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/042Introducing gases into the water, e.g. aerators, air pumps
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/047Liquid pumps for aquaria
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/06Arrangements for heating or lighting in, or attached to, receptacles for live fish
    • A01K63/065Heating or cooling devices
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Zoology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The invention belongs to the field of aquaculture, and particularly relates to a method for improving the survival rate of procambarus clarkii cultured in a laboratory, which comprises the following steps: (1) constructing a culture system of a reservoir, a water pump, a cooling and heating machine, an oxygenation pump and an air conditioner; the water inlet of the water pump is connected with the reservoir, the water outlet of the water pump is connected with the cooling and heating machine, and the water outlet of the cooling and heating machine is connected to the upper part of the culture box; the aerator aerates the water body of the reservoir and aerates the water body of the culture box; arranging 3 layers of culture frames, placing culture boxes on the culture frames, and arranging water inlets and oxygen increasing heads at the bottoms of the culture boxes; a sewage discharge pipe is connected below the box; a culture cage is arranged in the culture box; (2) controlling the water level of the culture box to 3/4 parts of the culture cage, and changing the water in the culture box to keep the water temperature at 21-26 ℃; (3) putting 0.3g of young shrimps into a perforated culture cage, and putting the adult shrimps into the culture cage with a lock catch and a top cover; (4) keeping the dissolved oxygen of the water body above 5 mg/L; feeding the feed properly, and keeping the illumination for more than 10 hours.

Description

Method for improving survival rate of procambarus clarkii cultured in laboratory
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to an indoor culture method for improving the survival rate of procambarus clarkia in a laboratory.
Background
Procambarus clarkii (Procambarus clarkii) is commonly known as crayfish, belonging to the Crustacea, the order decapod, the family Crabyssaceae, the genus Procambarus. Procambarus clarkii has the same habits of shelling, fighting and cannibalism, and in herd farming situations, greater mortality often occurs during shelling. In recent years, the scale and the yield of the procambarus clarkii are gradually enlarged in China, but the research on the protective culture of the procambarus clarkii is relatively weak, so that the method becomes a technical problem to be solved in the field.
At present, the domestic procambarus clarkii is mainly cultured in an outdoor pond or a rice shrimp field, the outdoor culture technology is relatively researched, and the indoor culture technology is relatively lack of research. The procambarus clarkii is treated in a laboratory by sampling from the field and temporarily culturing indoors mostly, and the experimental treatment is carried out at an individual level, so that the survival rate is ensured by avoiding fighting among groups in the experimental process, and the consistency of the culture environment is ensured to become a difficult problem in the research in the field.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for improving the survival rate of procambarus clarkia cultured in a laboratory aiming at the problems so as to solve the problem of the survival rate of the procambarus clarkia in the prior art. The method has the advantages of simple operation and easily obtained raw materials, can improve the survival rate of the procambarus clarkia to more than 73.3 percent, and has great popularization effect on vast experimental researchers.
In order to realize the purpose, the invention adopts the main technical scheme that: a method for improving the survival rate of procambarus clarkii cultured in a laboratory comprises the following specific steps:
a method for improving the survival rate of procambarus clarkii cultured in a laboratory comprises the following steps:
(1) constructing a culture system: the culture system comprises a reservoir, a water pump, a cooling and heating machine, an oxygenation pump and an air conditioner; the water inlet of the water pump is connected with the reservoir through a PVC pipe, the water outlet of the water pump is connected with the cooling and heating machine through a PVC pipe and controls the water temperature, and the water outlet of the cooling and heating machine is connected to the upper part of each culture box through a PVC pipe; the oxygen increasing machine is connected with the air head through an air pipe to aerate the water body of the reservoir, and is connected above each culture box through another air pipe to increase the oxygen of the water body of the culture boxes; the air conditioner is arranged in a laboratory, a culture frame 2 is arranged in the laboratory, the culture frame 2 is arranged in 3 layers, culture boxes 1 are symmetrically arranged on the culture frame, and a water inlet 3 and an oxygen increasing head are arranged above the culture boxes, namely the bottom of the culture box on the upper layer; 9; a sewage discharge pipe 4 is connected below the culture box, and a water stop valve (8) is connected on the water inlet 3 and the sewage discharge port; a breeding cage 5 with holes and a cover is arranged in the breeding box;
(2) water quality management: controlling the water level in the culture box to 3/4 height of the culture cage, and replacing the water in the culture box every 3-4 days, wherein the water temperature is controlled to be 21-26 ℃;
(3) putting the procambarus clarkii: putting procambarus clarkii with the weight of 0.3g into cultivation cages with holes, and putting one procambarus clarkii juvenile shrimp into each cultivation cage and covering the juvenile shrimps with a cage cover; if the fed procambarus clarkii is adult shrimps, the top cover (7) is buckled by the lock catch (6);
(4) cultivation management: oxygen is added all day long during the culture period, and the dissolved oxygen in the culture water is kept to be controlled to be more than 5 mg/L; feeding granulated feed with the crude protein content of 30 percent or more every day, wherein the feeding amount is 2.8 to 3.2 percent of the weight of the procambarus clarkia, the feeding amount is adjusted according to the eating condition of one continuous week, and the illumination time is kept for more than 10 hours every day.
Specifically, the specific details of the invention are as follows:
(1) building a culture rack and a culture box: the size of the culture rack is as follows: length, width, and height: 1.85m 0.6m 2.0m, preferably 3 layers, each layer symmetrically placing 2 rows of the cultivation boxes (see fig. 2). The specification of the culture box is length, width and height: 76.5cm 56.5cm 22.5 cm; the material is polyvinyl chloride material (PVC), and the colour is dark grey, breeds the bottom of the box and connects sewage pipes, is convenient for clear up. The related lighting lamp is additionally arranged above each culture box, and the water inlet pipeline is additionally arranged above the culture boxes and erected at the bottom of the culture box at the upper layer so as to increase the operation space. A plurality of cylindrical culture cages (the arrangement mode is shown in figure 3) can be placed in the culture box, holes (shown in figure 4) are formed in the periphery of each culture cage, the holes in the periphery are staggered and uniformly arranged, and the holes in the top and the bottom are annularly and uniformly arranged. The multiple holes (shown in figure 4) are formed around the culture cage, so that the culture water body environment in each culture cage in the same culture box is basically the same, meanwhile, the porous structure around is also favorable for the climbing of the Procambrus clarkii, and the porous structure at the bottom of the culture cage is favorable for the culture waste to sink to the bottom of the culture box.
(2) Controlling the water quality: ensuring that the water level in the culture box does not exceed the culture cage, controlling the water temperature to be 21-26 ℃, and replacing the culture water every 3-5 days.
(3) Cultivation management: starting an oxygenation system (continuously oxygenating all day to keep dissolved oxygen in the aquaculture water body at more than 5 mg/L) and a temperature control system (controlling the temperature of the aquaculture water body at 23-25 ℃), adjusting the temperature of the reservoir through a cooling machine and an warming machine, and stabilizing the temperature of the water in the indoor aquaculture box through an air conditioner. The feed is fed once every 8-9 am and once again 5-6 pm, the feed mainly comprises commercial feed, and the feeding amount can be adjusted according to the eating condition of the procambarus clarkii. The illumination time is ensured to be more than 10 hours per day.
Preferably, the diameter of the aperture of the cultivation cage is 1.2mm, so that most of commercially available feed cannot leak to the bottom of the cultivation cage from the aperture to cause feed waste.
Preferably, a cross square block is placed below the bottom of the breeding cage, so that the bottom of the breeding cage and the bottom of the breeding box keep a certain height, and dirt is convenient to clean.
Preferably, the water level in the culture box is controlled at the 3/4 height of the culture cage, so that the death caused by oxygen deficiency can be effectively reduced.
Compared with other existing technologies for improving the survival rate, the method has the following beneficial effects:
the cage with the holes can ensure that the water body environment of each shrimp in the same culture box is basically the same, and can distinguish each tail of the procambarus clarkii, thereby facilitating the observation of the effect of the procambarus clarkii after experimental treatment in a laboratory; the procambarus clarkii is independently separated, so that the phenomenon of mutual cannibalism among the same procambarus clarkii can be avoided during shelling, and the accuracy of data after experimental treatment is improved. The invention utilizes the biological habits of the procambarus clarkia to ensure that the procambarus clarkia can reduce the death caused by oxygen deficiency due to accidental damage of oxygen increasing equipment by fresh oxygen which is attached to the side wall of the culture cage to breathe air during the oxygen deficiency, thereby improving the survival rate. When decontaminating the breed box, can adopt different modes to decontaminate according to the experiment requirement, can also carry out the blowdown through the drain after can taking out the breed cage, also can clear up the waste material all around of breed cage through the mode of conventional siphon, also can directly carry out the blowdown through the drain when breeding the cage less.
Drawings
FIG. 1: the cultivation system of the invention is schematically shown.
FIG. 2: the invention discloses a culture rack structure and a culture box placing side schematic diagram.
FIG. 3: the invention relates to a top view of a culture cage placed in a culture box.
FIG. 4: the invention discloses a three-dimensional view of a cultivation cage.
FIG. 5: the front view structure of the cultivation cage is schematic.
FIG. 6: schematic structural diagram of a top view of the cultivation cage.
FIG. 7: the survival rate of the procambarus clarkii in the embodiment of the invention is high.
Description of reference numerals: 1-culture box, 2-culture frame, 3-water inlet pipe, 4-water outlet pipe, 5-culture cage, 6-lock catch, 7-culture cage top cover, 8-water stop valve and 9-oxygen aeration head.
Detailed description of the invention
Example 1:
a method for improving the survival rate of procambarus clarkii cultured in a laboratory comprises the following steps:
(1) constructing a culture system: the culture system comprises a reservoir, a water pump, a cooling and heating machine, an oxygenation pump and an air conditioner; the water inlet of the water pump is connected with the reservoir through a PVC pipe, the water outlet of the water pump is connected with the cooling and heating machine through a PVC pipe and controls the water temperature, and the water outlet of the cooling and heating machine is connected to the upper part of each culture box through a PVC pipe; the aerator is connected with the air head through an air pipe to aerate the water body of the reservoir and is connected above each culture box through another air pipe to aerate the water body of the culture boxes; the air conditioner is arranged in a laboratory, a culture frame 2 is arranged in the laboratory, the culture frame 2 is arranged in 3 layers, culture boxes 1 are symmetrically arranged on the culture frame, and a water inlet 3 and an oxygen increasing head are arranged above the culture boxes, namely the bottom of the culture box on the upper layer; 9; a sewage discharge pipe 4 is connected below the culture box, and a water stop valve 8 is connected on the water inlet 3 and the sewage discharge port; a breeding cage 5 with holes and a cover is arranged in the breeding box;
(2) water quality management: controlling the water level in the culture box to 3/4 height of the culture cage, and replacing the water in the culture box every 3-4 days, wherein the water temperature is controlled to be 21-26 ℃;
(3) putting the procambarus clarkii: putting the procambarus clarkii larvae with the weight of 0.3g into a cultivation cage with holes, and putting 1 tail of the procambarus clarkii larvae into each cultivation cage and covering the cultivation cages with a cage cover; if the fed procambarus clarkii is adult shrimps, the used culture cage is buckled on the top cover (7) by a lock catch (6);
(4) cultivation management: oxygen is increased all day long during the culture period, and the dissolved oxygen in the culture water is controlled to be more than 5 mg/L; feeding granulated feed with the crude protein content of 30 percent or more every day, wherein the feeding amount is 2.8 to 3.2 percent of the weight of the procambarus clarkia, the feeding amount is adjusted according to the eating condition of one continuous week, and the illumination time is kept for more than 10 hours every day.
As some of these examples. The following parameters illustrate specific conditions for the practice of the invention
The size of the cultivation frame is preferably length, width and height: 1.85m 0.6m 2.0m, for a total of 3 layers (fig. 2). The height between 2 adjacent layers is 60cm, and 4 breeding boxes are symmetrically placed on each layer, and the specification of the breeding boxes is preferably length, width and height: 76.5cm 56.5cm 22.5cm, the material is PVC, the color is preferably dark gray, each culture box is preferably provided with 20 culture cages (figure 3), and the size of each culture cage is preferably as follows: the diameter of the bottom is 14cm, the height is 16cm, the aperture is 1.2mm, and the two sides are provided with lock catches (figure 4 and figure 5) which are made of stainless steel.
(2) Feeding the cultured shrimps: the weight specification of procambarus clarkii is 0.31 +/-0.10 g of shrimp fries, 1 tail is put in each breeding cage, and 20 tails are put in each breeding box in 3 breeding boxes, and the total number of the breeding boxes is 60.
3) Controlling the water quality: the water level is controlled to be about 13cm, the water temperature is controlled to be 23-25 ℃, the water body in the culture box is replaced every 4 days, and the water temperature difference before and after replacement is not more than 3 ℃.
4) Cultivation management: continuously increasing oxygen throughout the day to ensure that the dissolved oxygen in the aquaculture water is kept above 5mg/L, feeding commercial procambarus clarkii feed with the crude protein content of 30 percent between 5 and 6 pm, wherein the feeding amount is 2 to 3 percent of the weight of the feed, and cleaning the feed which is not eaten up the day before feeding. If the same amount of the feed is not left in the continuous feeding for several days, the feeding amount is increased. The illumination time is guaranteed to be more than 10h every day.
In this embodiment, the cultivation effect of three cultivation boxes is shown in FIG. 7. In this example, a control group under normal free-range conditions was set, and the treatment conditions were the same except that no breeding cage was used (20 procambarus clarkii were placed in 3 breeding boxes). In the embodiment, the number and the corresponding survival rate of the remaining procambarus clarkii in different time periods are shown in 6 months of cultivation (fig. 7), the cultivation effect of 3 cultivation boxes in the embodiment is shown as cage cultivation 1, cage cultivation 2 and cage cultivation 3 in fig. 7, and the cultivation effect of a control group without cultivation cages is shown as mixed cultivation 1, mixed cultivation 2 and mixed cultivation 3 in fig. 7. The result shows that the average survival rate of the procambarus clarkii cage culture is 73.3 percent and is obviously higher than 26.67 percent of the average survival rate of the scatter culture, and the level reaches obvious (P is less than 0.05).

Claims (1)

1. A method for improving the survival rate of procambarus clarkii cultured in a laboratory is characterized by comprising the following steps:
(1) constructing a culture system: the culture system comprises a reservoir, a water pump, a cooling and heating machine, an oxygenation pump and an air conditioner; the water inlet of the water pump is connected with the reservoir through a PVC pipe, the water outlet of the water pump is connected with the cooling and heating machine through a PVC pipe and controls the water temperature, and the water outlet of the cooling and heating machine is connected to the upper part of each culture box through a PVC pipe; the oxygen increasing machine is connected with the air head through an air pipe to aerate the water body of the reservoir, and is connected above each culture box through another air pipe to increase the oxygen of the water body of the culture boxes; the air conditioner is arranged in a laboratory, a culture frame (2) is arranged in the laboratory, the culture frame (2) is arranged in 3 layers, the culture boxes (1) are symmetrically arranged on the culture frame, and a water inlet (3) and an oxygen increasing head (9) are arranged above the culture boxes, namely at the bottom of the culture box on the upper layer; a drain pipe (4) is connected below the culture box, and a water stop valve (8) is connected on the water inlet (3) and the drain outlet; a breeding cage (5) with holes and a cover is arranged in the breeding box;
(2) water quality management: controlling the water level in the culture box to 3/4 height of the culture cage, and replacing the water in the culture box every 3-4 days, wherein the water temperature is controlled to be 21-26 ℃;
(3) putting the procambarus clarkii: putting procambarus clarkia larvae with the weight of 0.3g into cultivation cages with holes, putting 1 tail in each cultivation cage, and covering the cages with a cage cover; the shrimp breeding cage is provided with a lock catch (6), and the lock catch (6) is used for buckling a top cover (7);
(4) cultivation management: oxygen is added all day long during the culture period, and the dissolved oxygen of the culture water is controlled to be more than 5 mg/L; feeding granulated feed with the crude protein content of 30 percent or more every day, wherein the feeding amount is 2.8 to 3.2 percent of the weight of the procambarus clarkia, the feeding amount is adjusted according to the eating condition of one continuous week, and the illumination time is kept over 10 hours every day.
CN202010568723.7A 2020-06-19 2020-06-19 Method for improving survival rate of procambarus clarkii cultured in laboratory Pending CN111700012A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114375894A (en) * 2022-01-27 2022-04-22 福建省农业科学院农业质量标准与检测技术研究所 Australia freshwater lobster breeding system and ecological breeding method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN204047622U (en) * 2014-07-25 2014-12-31 中国水产科学研究院东海水产研究所 The water circulation experimental provision of cultivation Scylla paramamosain
CN208300706U (en) * 2018-06-11 2019-01-01 湖北赤壁盛艺农业开发有限公司 Multi-functional underwater culture partition
CN109479776A (en) * 2018-12-03 2019-03-19 安徽省农业科学院水产研究所 A kind of batch production Procambius clarkii mating system
CN110338130A (en) * 2019-08-07 2019-10-18 中国水产科学研究院长江水产研究所 A kind of the Laboratory culture system and high-density breeding method of Procambius clarkii

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN204047622U (en) * 2014-07-25 2014-12-31 中国水产科学研究院东海水产研究所 The water circulation experimental provision of cultivation Scylla paramamosain
CN208300706U (en) * 2018-06-11 2019-01-01 湖北赤壁盛艺农业开发有限公司 Multi-functional underwater culture partition
CN109479776A (en) * 2018-12-03 2019-03-19 安徽省农业科学院水产研究所 A kind of batch production Procambius clarkii mating system
CN110338130A (en) * 2019-08-07 2019-10-18 中国水产科学研究院长江水产研究所 A kind of the Laboratory culture system and high-density breeding method of Procambius clarkii

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114375894A (en) * 2022-01-27 2022-04-22 福建省农业科学院农业质量标准与检测技术研究所 Australia freshwater lobster breeding system and ecological breeding method thereof

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