CN111690516A - Anaerobic bacteria culture sealing device - Google Patents
Anaerobic bacteria culture sealing device Download PDFInfo
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- CN111690516A CN111690516A CN202010778653.8A CN202010778653A CN111690516A CN 111690516 A CN111690516 A CN 111690516A CN 202010778653 A CN202010778653 A CN 202010778653A CN 111690516 A CN111690516 A CN 111690516A
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- 238000007789 sealing Methods 0.000 title claims abstract description 47
- 241001148471 unidentified anaerobic bacterium Species 0.000 title abstract description 12
- 230000001580 bacterial effect Effects 0.000 claims description 10
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000741 silica gel Substances 0.000 claims description 6
- 229910002027 silica gel Inorganic materials 0.000 claims description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract description 20
- 239000001301 oxygen Substances 0.000 abstract description 20
- 229910052760 oxygen Inorganic materials 0.000 abstract description 20
- 241000894006 Bacteria Species 0.000 abstract description 18
- 239000007789 gas Substances 0.000 abstract description 15
- 238000004519 manufacturing process Methods 0.000 abstract description 11
- 230000036284 oxygen consumption Effects 0.000 abstract description 4
- 238000012258 culturing Methods 0.000 abstract description 3
- 239000002775 capsule Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 14
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 8
- 238000005457 optimization Methods 0.000 description 6
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 4
- 229910002092 carbon dioxide Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 238000006555 catalytic reaction Methods 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 229910052763 palladium Inorganic materials 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical group [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000002026 carminative effect Effects 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention provides an anaerobic bacteria culture sealing device, which comprises an outer box body, wherein the outer box body is a box body with an open upper part, two opposite side surfaces of the outer box body are respectively provided with a vent valve inlet and a vent valve outlet, the upper end surface of the outer box body is connected with a sealing cover, the device can be divided into two operation modes, when strict anaerobic bacteria are cultured, a digital display controller, an anaerobic gas production bag and bacteria to be cultured are placed in the outer box body, the sealing cover is covered, the anaerobic gas production bag consumes oxygen to generate an anaerobic environment for culturing the anaerobic bacteria, when the micro-aerobic bacteria are cultured, the digital display controller, a controllable sealing small box and the bacteria to be cultured are placed in the outer box body, the digital display controller and the controllable sealing small box are connected through control lines, the anaerobic gas production bag is placed in the controllable sealing small box, the sealing cover is covered, the anaerobic gas production bag consumes oxygen, and when a set oxygen value is reached, the digital display controller controls the, the anaerobic gas production bag stops reaction, generates a micro oxygen consumption environment, and micro oxygen consumption bacteria grow.
Description
Technical Field
The invention belongs to the technical field of bacterial culture, and particularly relates to an anaerobic bacterial culture sealing device.
Background
The bacteria are widely distributed in nature, large in quantity and multiple in types, can benefit mankind, and can also become a cause of diseases. Bacterial culture is a technique in which bacteria are grown and propagated artificially. Most of the bacteria can be cultured by an artificial method, namely, the bacteria are inoculated on a culture medium and are grown and propagated, and the cultured bacteria are used for research, identification and application. Anaerobes are a group of bacteria that can only grow under conditions of low oxygen partial pressure. They can be classified into obligate anaerobes, microaerophilic anaerobes and facultative anaerobes according to their tolerance to oxygen. Common anaerobic culture methods are a candler method, a pyrogallic method, a palladium catalysis method, an anaerobic tank air extraction method and the like, and the methods have high requirements on containers, need high-temperature resistant containers, or need to add water and catalysts, and have complex operation. How to provide anaerobic or micro-aerobic environment for bacteria during bacteria culture is a problem to be solved urgently.
Disclosure of Invention
The invention aims to provide an anaerobic bacteria culture sealing device to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: an anaerobic bacteria culture sealing device comprises an outer box body, wherein the outer box body is a box body with an open upper part, two opposite side surfaces of the outer box body are respectively provided with a vent valve inlet and a vent valve outlet, the upper end surface of the outer box body is connected with a sealing cover, the vent valve inlet can be positioned at the upper part of the side surface of the outer box body, the vent valve outlet can be positioned at the lower part of the side surface of the outer box body, the upper part of the outer box body is open, the inner part of the outer box body is of a hollow structure, and the upper end surface of the outer box body is connected with the sealing;
the inner side surface of the outer box body is connected with a digital display control instrument, a control instrument output port and a charging socket are respectively arranged on the digital display control instrument, the charging socket is electrically connected with an external power supply, the digital display control instrument monitors the temperature, the humidity, the air pressure, the oxygen content, the carbon dioxide content and the hydrogen content in the outer box body, a sucker is arranged on the front surface of the digital display control instrument, can be fixed on the side wall or the top cover in the outer box body in an absorption way, the digital display control instrument can be connected with a mobile phone or a computer through Bluetooth or WiFi, the digital display control instrument has recording capability, historical values of all parameters can be checked at a mobile phone or a computer end, an oxygen content sensor, a temperature and humidity sensor and the like can also be fixedly installed on the inner side surface of the outer box body, the oxygen content sensor is connected with the temperature and humidity sensor and a digital display controller, the oxygen content sensor is a sensor of Xiangfeng automobile distribution limited company in Guangzhou city, for example, and a temperature and humidity detector is a sensor with the model number of RSDS12, for example;
the inner side surface of the outer box body is provided with a controllable sealed capsule which comprises a flip cover, a sealed capsule shell and a sealed capsule input interface, the sealed capsule shell is a shell with an open upper part, the sealed capsule shell is fixedly arranged at the bottom side in the outer box body through a fixing frame, the upper side of the sealed capsule shell is connected with the flip cover, the bottom side of the sealed capsule shell is provided with the sealed capsule input interface, the sealed capsule input interface is connected with an output port of a controller through a control line, one end of the flip cover is hinged with the upper side of the sealed capsule shell, the other end of the flip cover is magnetically connected with the upper side of the sealed capsule shell, for example, the side surface of the flip cover can be provided with a first magnet, the side surface of the sealed capsule shell is provided with a second magnet, the first magnet is magnetically connected with the second magnet, the fixing frame can be fixedly arranged at the inner side of the outer, thereby realizing the installation of the controllable sealing small box.
As optimization, a fan is installed inside the digital display controller, for example, the fan is selected from Shenzhen Shensheng motor Limited company with the model number of FS12038, can be started at regular time, ensures that the gas inside the outer box body is uniformly distributed, and can also be used for radiating the digital display controller.
As optimization, a battery is installed inside the digital display control instrument and can be charged through a charging socket, and the battery is a lithium battery with the model number of DL-D-SY-5AH, for example.
As optimization, the digital display control appearance accessible control flap opens and shuts, for example, sealed capsule shell medial surface fixed mounting has the electric jar, sealed capsule input interface and controller delivery outlet pass through the control line and connect, sealed capsule input interface passes through electric wire and electric jar electric connection, the work of digital display control appearance control electric jar to the opening and shutting of control flap, the flexible end of electric jar is close to and keeps away from flip, thereby realize flip's opening and shutting, the electric jar for example chooses the electric jar that the model is DSDG25, the digital display control appearance for example chooses the controller that the model is MF4600 for example.
As optimization, still be equipped with the display screen on the digital display control appearance, pilot lamp and operating button all are located the display screen side, the display screen, pilot lamp and operating button respectively with digital display control appearance internally mounted's battery electric connection, digital display control appearance monitoring outer box internal temperature, humidity, atmospheric pressure, oxygen content, carbon dioxide content, hydrogen content, controller delivery outlet and display screen are connected, digital display control appearance can pass through bluetooth or wiFi and connect cell-phone or computer, can look over the numerical value change of each parameter through cell-phone end or computer end, this device requires lowly to the container, can replace the outer box with the plastic box that the leakproofness is better, and easy operation has solved other cultivation methods and culture apparatus and has required the high to the container, the problem of complicated operation.
As optimization, the upper end face of the small sealed box shell is bonded with the silica gel sealing strip, and the silica gel sealing strip is bonded around the upper end face of the small sealed box shell, so that after the flip cover is connected with the bayonet of the small sealed box shell, the inside of the small sealed box shell can be sealed due to the arrangement of the silica gel sealing strip, and the small controllable sealed box is positioned on one side of the digital display controller.
As optimization, the upper side surface of the outer box body is provided with a handle.
Compared with the prior art, the invention has the beneficial effects that:
in the invention, a sealed environment is formed by the outer box body and the sealing cover, an anaerobic gas generation bag is arranged in the outer box body to consume oxygen so as to form a micro-aerobic or anaerobic environment, parameters such as oxygen content, carbon dioxide content and the like are monitored by a digital display control instrument, and the oxygen content is controlled by opening and closing the controllable small sealing box.
Compared with common anaerobic culture methods such as a candler method, a pyrogallic method, a palladium catalysis method and the like, the method has low requirements on the container, few requirements on reagent consumables and simpler operation.
Drawings
FIG. 1 is a schematic view of the overall structure of an anaerobic bacteria culture sealing device according to the present invention;
FIG. 2 is a schematic structural view of a controllable sealing capsule according to the present invention;
FIG. 3 is a schematic view of a part of the digital display control instrument according to the present invention;
FIG. 4 is a schematic diagram of a part of the digital display control instrument of the invention.
In the figure: 1. an outer case; 2. a vent valve inlet; 3. a digital display control instrument; 3-2, displaying screen; 3-4, an indicator light; 3-5, operating a button; 3-6, a fan; 3-10, a control instrument output port; 3-11, a charging socket; 4. sealing the cover; 6. a handle; 7. a controllable sealed capsule; 7-1, turning over a cover; 7-2, a silica gel sealing strip; 7-3, sealing the small box shell; 7-5, sealing the input interface of the small box; 8. a fixed mount; 9 vent valve outlet.
Detailed Description
The present invention will be further illustrated with reference to the accompanying drawings and specific embodiments, which are to be understood as merely illustrative of the invention and not as limiting the scope of the invention. It should be noted that the terms "front," "back," "left," "right," "upper" and "lower" used in the following description refer to directions in the drawings, and the terms "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.
Referring to fig. 1-4, an anaerobic bacteria culture sealing device comprises an outer box 1, wherein the outer box 1 is a box with an open upper part, two opposite side surfaces of the outer box 1 are respectively provided with a vent valve inlet 2 and a vent valve outlet 9, and the upper end surface of the outer box 1 is connected with a sealing cover 4;
the inner side surface of the outer box body 1 is connected with a digital display control instrument 3, the digital display control instrument 3 is respectively provided with a control instrument output port 3-10 and a charging socket 3-11, and the charging socket 3-11 is electrically connected with an external power supply;
the inner side surface of the outer box body 1 is provided with a controllable small sealed box 7, the controllable small sealed box 7 comprises a flip cover 7-1, a small sealed box shell 7-3 and a small sealed box input interface 7-5, the small sealed box shell 7-3 is a shell with an open upper part, the small sealed box shell 7-3 is fixedly arranged at the bottom side inside the outer box body 1 through a fixing frame 8, the flip cover 7-1 is connected to the upper side of the small sealed box shell 7-3, the small sealed box input interface 7-5 is arranged at the bottom side of the small sealed box shell 7-3, and the small sealed box input interface 7-5 is connected with the output port 3-10 of the control instrument through a control line.
The digital display control instrument 3 is internally provided with fans 3-6.
The digital display control instrument 3 is internally provided with a battery which can be charged through the charging sockets 3-11.
The digital display control instrument 3 can control the opening and closing of the flip cover 7-1 through a control line.
The digital display control instrument 3 is also provided with a display screen 3-2, an indicator lamp 3-4 and an operation button 3-5, the indicator lamp 3-4 and the operation button 3-5 are both positioned on the side surface of the display screen 3-2, and the display screen 3-2, the indicator lamp 3-4 and the operation button 3-5 are respectively and electrically connected with a battery arranged inside the digital display control instrument 3.
The upper end surface of the small sealing box shell 7-3 is bonded with a silica gel sealing strip 7-2.
The upper side surface of the outer box body 1 is provided with a handle 6.
The working principle is as follows:
the invention provides an anaerobic bacteria culture sealing device, which comprises an outer box body 1, a digital display controller 3, a controllable small sealed box 7 and an anaerobic gas production bag, wherein the outer box body 1 is a box body of which the upper part is connected with a sealing cover 4 in a buckling mode, the digital display controller 3 can measure parameters such as oxygen content and the like, the digital display controller 3 controls the controllable small sealed box 7 to be opened and closed to control the oxygen content, the anaerobic gas production bag consumes oxygen in the outer box body, and an anaerobic environment is created;
the device can be divided into two operation modes, when culturing strict anaerobic bacteria, a digital display controller 3 is adsorbed and fixed on the inner wall of an outer box body 1, an anaerobic gas production bag and bacteria to be cultured are also placed in the outer box body 1, a sealing cover 4 is covered, the anaerobic gas production bag consumes oxygen, an anaerobic environment is generated, the anaerobic bacteria are cultured, the digital display controller 3 monitors the temperature, the humidity, the air pressure, the oxygen content, the carbon dioxide content and the hydrogen content in the outer box body, the environment is ensured to be stable, when culturing the micro-aerobic bacteria, an oxygen threshold value is set on the digital display controller 3, the digital display controller 3 is adsorbed and fixed on the inner wall of the outer box body 1, a controllable sealing small box 7 and the bacteria to be cultured are placed in the outer box body 1, the digital display controller 3 and the controllable sealing small box 7 are connected through a control line, the anaerobic gas production bag is placed in the controllable sealing small box 7, the sealing cover 4 is covered, the digital display controller 3 monitors the temperature in, when the oxygen content reaches a threshold value, the digital display control instrument 3 controls the controllable small sealed box 7 to be closed, the anaerobic gas production bag stops reacting, a micro oxygen consumption environment is generated, and micro oxygen consumption bacteria grow;
outer box 1 side upper portion is equipped with breather valve import 2, and the offside lower part is equipped with breather valve export 9, and when cultivateing some bacteria that need special gaseous condition, the breather valve of accessible both sides, one end advance required gas, and the other end is carminative, and digital display control appearance 3 monitors gas content, when reaching the requirement, closes both ends breather valve, carries out the bacteria cultivation.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents, and it is intended that the described embodiments be construed as merely a subset of the embodiments of the invention and not as a whole. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Claims (7)
1. The utility model provides an anaerobism bacterial culture sealing device, includes outer box (1), outer box (1) is the open box in upper portion, its characterized in that: two opposite side surfaces of the outer box body (1) are respectively provided with a vent valve inlet (2) and a vent valve outlet (9), and the upper end surface of the outer box body (1) is connected with a sealing cover (4);
the inner side surface of the outer box body (1) is connected with a digital display control instrument (3), a control instrument output port (3-10) and a charging socket (3-11) are respectively arranged on the digital display control instrument (3), and the charging socket (3-11) is electrically connected with an external power supply;
the inner side surface of the outer box body (1) is provided with a controllable small sealed box (7), the controllable small sealed box (7) comprises a flip cover (7-1), a small sealed box shell (7-3) and a small sealed box input interface (7-5), the small sealed box shell (7-3) is a shell with an open upper portion, the small sealed box shell (7-3) is fixedly installed on the bottom side of the inner portion of the outer box body (1) through a fixing frame (8), the flip cover (7-1) is connected to the upper side of the small sealed box shell (7-3), the small sealed box input interface (7-5) is arranged on the bottom side of the small sealed box shell (7-3), and the small sealed box input interface (7-5) is connected with a controller output port (3-10) through a control line.
2. The anaerobic bacterial culture sealing device of claim 1, wherein: and a fan (3-6) is arranged in the digital display control instrument (3).
3. The anaerobic bacterial culture sealing device of claim 1, wherein: the digital display control instrument (3) is internally provided with a battery, and the battery can be charged through the charging sockets (3-11).
4. The anaerobic bacterial culture sealing device of claim 1, wherein: the digital display control instrument (3) can control the opening and closing of the flip cover (7-1) through a control line.
5. The anaerobic bacterial culture sealing device of claim 3, wherein: the digital display control instrument (3) is further provided with a display screen (3-2), an indicator lamp (3-4) and an operating button (3-5), the indicator lamp (3-4) and the operating button (3-5) are located on the side face of the display screen (3-2), and the display screen (3-2), the indicator lamp (3-4) and the operating button (3-5) are respectively electrically connected with a battery arranged inside the digital display control instrument (3).
6. The anaerobic bacterial culture sealing device of claim 1, wherein: and a silica gel sealing strip (7-2) is bonded on the upper end surface of the small sealing box shell (7-3).
7. The anaerobic bacterial culture sealing device of claim 1, wherein: the upper side surface of the outer box body (1) is provided with a handle (6).
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| CN202010778653.8A CN111690516A (en) | 2020-08-05 | 2020-08-05 | Anaerobic bacteria culture sealing device |
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| DE10236637B3 (en) * | 2002-08-09 | 2004-01-22 | Michael Oliveri | Method and device for measuring the amount of biogas |
| JP2008029332A (en) * | 2006-06-29 | 2008-02-14 | Higashi Nippon Gakuen Hokkaido Iryo Daigaku | Vessel for anaerobic bacterium culture kit and the anaerobic bacterium culture kit, and method for culturing anaerobic bacterium and method for discriminating growth of anaerobic bacterium |
| CN203424115U (en) * | 2013-08-28 | 2014-02-12 | 金陵科技学院 | Pet cage with temperature alarm device |
| CN205115447U (en) * | 2015-10-20 | 2016-03-30 | 青岛高科技工业园海博生物技术有限公司 | It produces air pocket to consume oxygen |
| CN209276529U (en) * | 2018-12-12 | 2019-08-20 | 桂林电子科技大学 | A kind of vertical multi-stage anaerobic reactor |
| CN210458198U (en) * | 2019-04-09 | 2020-05-05 | 安徽金种子酒业股份有限公司 | Laboratory is with little aerobic microorganism culture apparatus |
| CN210176859U (en) * | 2019-04-29 | 2020-03-24 | 翟卫华 | Biogas temperature control and heating system |
| CN212404087U (en) * | 2020-08-05 | 2021-01-26 | 金陵科技学院 | Anaerobic bacteria culture sealing device |
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Application publication date: 20200922 |