CN111671808A - Antiviral noni SOD oral spray and preparation method thereof - Google Patents

Abstract

The invention discloses an antiviral noni SOD oral cavity spray, which belongs to the technical field of spray processing and is prepared from the following substances: noni fruit extract micropowder, herba asari, herba Andrographitis, radix Platycodi, herba Menthae, Glycyrrhrizae radix, herba Taraxaci, Scutellariae radix, Coptidis rhizoma, and water. The oral spray has the advantages that the reasonable compatibility improvement is carried out on the raw material components of the oral spray, the use quality of the oral spray is well improved, the oral spray has obvious antibacterial and antiviral effects, and the effects of moistening lung for arresting cough, clearing heat and promoting fluid, and detoxifying and reducing swelling are realized.

Description

Antiviral noni SOD oral spray and preparation method thereof

Technical Field

The invention belongs to the technical field of spray processing, and particularly relates to an antiviral noni SOD oral spray.

Background

Noni (Morinda citrifolia Linn), a tropical evergreen perennial broadleaf shrub or small arbor originating from islands of the south pacific ocean, is a plant of the Morinda genus of the rubiaceae family. Noni fruit is called 'super fruit', is rich in 227 nutrient components, comprises 13 vitamins (A, B, C, E and the like), 16 minerals (potassium, sodium, zinc, calcium, iron, magnesium, phosphorus, copper, selenium and the like), 8 trace elements, more than 20 amino acids including 9 amino acids essential to human body (the total content of the amino acids is the first content in 16 tropical fruits, and the super combination containing the most amino acids in plants) and a plurality of substances with antioxidant effect. Also contains medicinal components such as belladonna, various compounds, various green onion wakening compounds, various alkaloids, sitosterol, various flavone glycosides, alizarin, rutin and the like, and has various effects of resisting aging, bacteria, viruses, tumors and parasites, relieving pain, reducing blood pressure, diminishing inflammation, improving immunity and the like. In 6 months and 1 day 2010, noni pulp is approved as new resource food by No. 9 notice of Ministry of health of China, the safety of noni pulp is fully demonstrated, and no toxic or side effect is caused after long-term use.

The body is directly or indirectly injured by multiple pathogenic factors such as physics, chemistry, bacteria, viruses and the like, firstly, the oral cavity, the throat and the upper respiratory tract of a human body are impacted, and the harmful factors cause oral mucosa injury, inflammation, ulcer, odontitis, acute and chronic pharyngolaryngitis, lymphoplasia, sore throat, hoarseness, foreign body sensation in the throat, itching and pain sensation in the throat, difficulty in swallowing, dry mouth and tongue, and the disease course is long, difficult to cure and repeatedly attack. Various even unknown pathogenic factors continuously injure the upper respiratory tract of people. Therefore, experts in the medical field have studied and developed many excellent drugs or functional health products for preventing and treating oral and throat diseases. However, because patients abuse antibiotic lozenges widely for a long time, even the patients abuse the antibiotic lozenges, germs and viruses in oral cavity, throat and upper respiratory tract already form stubborn drug resistance, and the drugs are continuously applied without obvious curative effect; secondly, clinical observation for many years proves that most of buccal tablets are not ideal in formula design and poor in clinical curative effect. Therefore, there is an urgent need to develop new products for preventing and treating oral diseases. At present, reports about the field of applying noni fruit processing to oral cavity sprays and the like for preventing and treating oral diseases are rarely seen.

Disclosure of Invention

The invention aims to provide an antiviral noni SOD oral spray and a preparation method thereof.

The technical purpose of the invention is realized by the following technical scheme:

an antiviral noni SOD oral cavity spray is prepared from the following substances in parts by weight:

7-10 parts of noni fruit extract micro powder, 5-8 parts of asarum, 3-6 parts of andrographis paniculata, 2-5 parts of platycodon grandiflorum, 1-4 parts of mint, 5-7 parts of liquorice, 2-6 parts of dandelion, 1-3 parts of scutellaria baicalensis, 1-3 parts of coptis chinensis and 900-1000 parts of water.

Preferably, the food is prepared from the following substances in parts by weight:

9 parts of noni fruit extract micro powder, 7 parts of asarum, 5 parts of common andrographis herb, 4 parts of platycodon grandiflorum, 3 parts of mint, 6 parts of liquorice, 4 parts of dandelion, 2 parts of scutellaria baicalensis, 2 parts of coptis chinensis and 960 parts of water.

Further, the preparation method of the noni fruit extract micro powder comprises the following steps:

1) washing noni fruits, soaking in ice water for 40-50 min, and taking out for later use;

2) drying the noni fruits treated in the step 1), and crushing the noni fruits to obtain crushed materials for later use;

3) immersing the crushed material obtained in the step 2) into an extracting solution, performing ultrasonic leaching treatment for 10-14 h, and performing suction filtration to obtain a suction filtration solution for later use;

4) carrying out high-speed centrifugation treatment on the suction filtration liquid obtained in the step 3), and removing centrifugal precipitates to obtain a centrifugal liquid for later use;

5) sequentially carrying out rough filtration and ultrafiltration treatment on the centrifugate obtained in the step 4) to obtain a fine filtrate for later use;

6) freeze-drying the fine filtrate obtained in the step 5) to obtain the noni fruit extract micro powder.

Further, the temperature of the drying treatment in the step 2) is not higher than 50 ℃; the particle size of the crushed materials is 150 meshes.

Further, the extracting solution in the step 3) is composed of the following substances in parts by weight: 2-2.5 parts of chitosan, 0.5-1.5 parts of tocopherol and 70-75 parts of edible alcohol; the concentration of the edible alcohol is 60%; the using amount of the extracting solution is 9-10 times of the total mass of the crushed materials; and the ultrasonic frequency is controlled to be 500-600 kHz during ultrasonic leaching treatment.

Further, the rotating speed of the high-speed centrifugation in the step 4) is controlled to be 6000-6500 rpm.

Further, the aperture of the filter membrane used for rough filtration in the step 5) is 15 μm, and the aperture of the filter membrane used for ultrafiltration is 0.2 μm.

Further, the freezing temperature in the freeze-drying treatment in the step 6) is-30 to-35 ℃.

A preparation method of an antiviral noni SOD oral spray comprises the following steps:

(1) firstly, mixing asarum, common andrographis herb, platycodon grandiflorum, mint, liquorice, dandelion, scutellaria baicalensis and coptis chinensis together, then putting into a pot containing wheat bran, continuously stir-frying for 50-55 min, taking out after completion, and removing redundant wheat bran to obtain a bran-fried medicine for later use;

(2) crushing the bran-fried medicine obtained in the step (1), then putting the crushed bran-fried medicine into clear water with the weight 5-7 times of the total weight of the bran-fried medicine, boiling the clear water for 1.5-2 hours, filtering the boiled clear water, and then concentrating the filtrate to 1/10 of the original volume for later use;

(3) and (3) mixing the noni fruit extract micro powder, the concentrated solution prepared in the step (2) and water, and fully and uniformly stirring to obtain the finished product of the oral spray.

Further, the temperature in the pot is controlled to be 110-120 ℃ during the stir-frying treatment in the step (1).

SOD, Superoxide Dismutase (SOD), also called antioxidant enzyme, widely exists in the organism in nature, can catalyze the disproportionation reaction of Superoxide radicals in the organism, and has the capability of scavenging free radicals. With the increase of people's age, the SOD content and activity in the skin tissue of the organism are obviously reduced, so that superoxide radical-aging factor in the body is continuously generated and accumulated.

Compared with the prior art, the invention has the following advantages:

the oral spray has the advantages that the reasonable compatibility improvement is carried out on the raw material components of the oral spray, the use quality of the oral spray is well improved, the oral spray has obvious antibacterial and antiviral effects, and the effects of moistening lung for arresting cough, clearing heat and promoting fluid, and detoxifying and reducing swelling are realized; the oral cavity cleaning agent can form a layer of protective film after being sprayed in the oral cavity, reduces further damage of pathogenic factors, promotes the quick repair of damaged tissues, reconstructs an immune system, is an excellent product for cleaning and preventing the oral cavity, and has great market competitiveness and popularization and application values.

Detailed Description

Example 1

An antiviral noni SOD oral cavity spray is prepared from the following substances in parts by weight:

7 parts of noni fruit extract micro powder, 5 parts of asarum, 3 parts of common andrographis herb, 2 parts of platycodon grandiflorum, 1 part of mint, 5 parts of liquorice, 2 parts of dandelion, 1 part of scutellaria baicalensis, 1 part of coptis chinensis and 900 parts of water.

The preparation method of the noni fruit extract micro powder comprises the following steps:

1) washing noni fruits, soaking in ice water for 40min, and taking out for later use;

2) drying the noni fruits treated in the step 1), and crushing the noni fruits to obtain crushed materials for later use;

3) immersing the crushed material obtained in the step 2) into an extracting solution, performing ultrasonic leaching treatment for 10 hours, and performing suction filtration to obtain a suction filtration solution for later use;

4) carrying out high-speed centrifugation treatment on the suction filtration liquid obtained in the step 3), and removing centrifugal precipitates to obtain a centrifugal liquid for later use;

5) sequentially carrying out rough filtration and ultrafiltration treatment on the centrifugate obtained in the step 4) to obtain a fine filtrate for later use;

6) freeze-drying the fine filtrate obtained in the step 5) to obtain the noni fruit extract micro powder.

The temperature of the drying treatment in the step 2) is not higher than 50 ℃; the particle size of the crushed materials is 150 meshes.

The extracting solution in the step 3) consists of the following substances in parts by weight: 2 parts of chitosan, 0.5 part of tocopherol and 70 parts of edible alcohol; the concentration of the edible alcohol is 60%; the using amount of the extracting solution is 9 times of the total mass of the crushed materials; and the ultrasonic frequency is controlled to be 500kHz during the ultrasonic leaching treatment.

Controlling the rotating speed of the centrifugation to be 6000rpm during the high-speed centrifugation treatment in the step 4).

The aperture of the filter membrane adopted during rough filtration in the step 5) is 15 μm, and the aperture of the filter membrane adopted during ultrafiltration is 0.2 μm.

The freezing temperature in the freeze-drying treatment in the step 6) is-30 ℃.

Example 2

An antiviral noni SOD oral cavity spray is prepared from the following substances in parts by weight:

9 parts of noni fruit extract micro powder, 7 parts of asarum, 5 parts of common andrographis herb, 4 parts of platycodon grandiflorum, 3 parts of mint, 6 parts of liquorice, 4 parts of dandelion, 2 parts of scutellaria baicalensis, 2 parts of coptis chinensis and 960 parts of water.

The preparation method of the noni fruit extract micro powder comprises the following steps:

1) washing noni fruits, soaking in ice water for 45min, and taking out for later use;

2) drying the noni fruits treated in the step 1), and crushing the noni fruits to obtain crushed materials for later use;

3) immersing the crushed material obtained in the step 2) into an extracting solution, performing ultrasonic leaching treatment for 12 hours, and performing suction filtration to obtain a suction filtration solution for later use;

4) carrying out high-speed centrifugation treatment on the suction filtration liquid obtained in the step 3), and removing centrifugal precipitates to obtain a centrifugal liquid for later use;

5) sequentially carrying out rough filtration and ultrafiltration treatment on the centrifugate obtained in the step 4) to obtain a fine filtrate for later use;

6) freeze-drying the fine filtrate obtained in the step 5) to obtain the noni fruit extract micro powder.

The temperature of the drying treatment in the step 2) is not higher than 50 ℃; the particle size of the crushed materials is 150 meshes.

The extracting solution in the step 3) consists of the following substances in parts by weight: 2.3 parts of chitosan, 1 part of tocopherol and 73 parts of edible alcohol; the concentration of the edible alcohol is 60%; the using amount of the extracting solution is 9.5 times of the total mass of the crushed materials; and the ultrasonic frequency is controlled to be 550kHz during the ultrasonic leaching treatment.

Controlling the rotation speed of the centrifugation to be 6300rpm during the high-speed centrifugation treatment in the step 4).

The aperture of the filter membrane adopted during rough filtration in the step 5) is 15 μm, and the aperture of the filter membrane adopted during ultrafiltration is 0.2 μm.

The freezing temperature in the freeze-drying treatment in the step 6) is-32 ℃.

Example 3

An antiviral noni SOD oral cavity spray is prepared from the following substances in parts by weight:

10 parts of noni fruit extract micro powder, 8 parts of asarum, 6 parts of common andrographis herb, 5 parts of platycodon grandiflorum, 4 parts of mint, 7 parts of liquorice, 6 parts of dandelion, 3 parts of scutellaria baicalensis, 3 parts of coptis chinensis and 1000 parts of water.

The preparation method of the noni fruit extract micro powder comprises the following steps:

1) washing noni fruits, soaking in ice water for 50min, and taking out for later use;

2) drying the noni fruits treated in the step 1), and crushing the noni fruits to obtain crushed materials for later use;

3) immersing the crushed material obtained in the step 2) into an extracting solution, performing ultrasonic leaching treatment for 14 hours, and performing suction filtration to obtain a suction filtration solution for later use;

4) carrying out high-speed centrifugation treatment on the suction filtration liquid obtained in the step 3), and removing centrifugal precipitates to obtain a centrifugal liquid for later use;

5) sequentially carrying out rough filtration and ultrafiltration treatment on the centrifugate obtained in the step 4) to obtain a fine filtrate for later use;

6) freeze-drying the fine filtrate obtained in the step 5) to obtain the noni fruit extract micro powder.

The temperature of the drying treatment in the step 2) is not higher than 50 ℃; the particle size of the crushed materials is 150 meshes.

The extracting solution in the step 3) consists of the following substances in parts by weight: 2.5 parts of chitosan, 1.5 parts of tocopherol and 75 parts of edible alcohol; the concentration of the edible alcohol is 60%; the using amount of the extracting solution is 10 times of the total mass of the crushed materials; and the ultrasonic frequency is controlled to be 600kHz during the ultrasonic leaching treatment.

Controlling the rotating speed of the centrifugation to be 6500rpm during the high-speed centrifugation treatment in the step 4).

The aperture of the filter membrane adopted during rough filtration in the step 5) is 15 μm, and the aperture of the filter membrane adopted during ultrafiltration is 0.2 μm.

The freezing temperature in the freeze-drying treatment in the step 6) is-35 ℃.

Example 4

A preparation method of an antiviral noni SOD oral spray comprises the following steps:

(1) mixing herba asari, herba Andrographitis, radix Platycodi, herba Menthae, Glycyrrhrizae radix, herba Taraxaci, Scutellariae radix and Coptidis rhizoma, adding into a pan containing testa Tritici, controlling the temperature in the pan at 110 deg.C, continuously stir-frying for 50min, taking out, and removing excessive testa Tritici to obtain bran-fried medicine;

(2) crushing the bran-fried medicine obtained in the step (1), then putting the crushed bran-fried medicine into clear water with the weight 5 times of the total weight of the bran-fried medicine, boiling the clear water for 1.5 hours, filtering the boiled bran-fried medicine, and then concentrating the filtrate to 1/10 of the original volume for later use;

(3) and (3) mixing the noni fruit extract micro powder, the concentrated solution prepared in the step (2) and water, and fully and uniformly stirring to obtain the finished product of the oral spray.

Example 5

A preparation method of an antiviral noni SOD oral spray comprises the following steps:

(1) mixing herba asari, herba Andrographitis, radix Platycodi, herba Menthae, Glycyrrhrizae radix, herba Taraxaci, Scutellariae radix and Coptidis rhizoma, adding into a pan containing testa Tritici, controlling the temperature in the pan to 115 deg.C, continuously stir-frying for 52min, taking out, and removing excessive testa Tritici to obtain bran-fried medicine;

(2) crushing the bran-fried medicine obtained in the step (1), then putting the crushed bran-fried medicine into clear water with the total mass being 6 times of that of the bran-fried medicine, boiling the clear water for 1.8 hours, filtering the boiled bran-fried medicine, and then concentrating the filtrate to 1/10 of the original volume for later use;

(3) and (3) mixing the noni fruit extract micro powder, the concentrated solution prepared in the step (2) and water, and fully and uniformly stirring to obtain the finished product of the oral spray.

Example 6

A preparation method of an antiviral noni SOD oral spray comprises the following steps:

(1) mixing herba asari, herba Andrographitis, radix Platycodi, herba Menthae, Glycyrrhrizae radix, herba Taraxaci, Scutellariae radix and Coptidis rhizoma, adding into a pan containing testa Tritici, controlling the temperature in the pan at 120 deg.C, continuously stir-frying for 55min, taking out and removing excessive testa Tritici to obtain bran-fried medicine;

(2) crushing the bran-fried medicine obtained in the step (1), then putting the crushed bran-fried medicine into clear water with the weight 5-7 times of the total weight of the bran-fried medicine, boiling for 2 hours, filtering, and concentrating the filtrate to 1/10 of the original volume for later use;

(3) and (3) mixing the noni fruit extract micro powder, the concentrated solution prepared in the step (2) and water, and fully and uniformly stirring to obtain the finished product of the oral spray.

Comparative example 1

This comparative example 1 is different from example 3 only in that the chitosan and tocopherol components in the extract of step 3) are omitted in the preparation of the Morinda citrifolia extract micropowder, except that the process steps are the same.

Comparative example 2

This comparative example 2 is different from example 3 only in that the ingredients of Andrographis paniculata and Platycodon grandiflorum are omitted, except that the steps of the method are the same.

Comparative example 3

This comparative example 3 is different from example 3 only in that the component of the Morinda citrifolia extract micropowder is omitted, except that the steps of the method are the same.

Test example 1

Staphylococcus aureus (purchased from ATCC, Cat. No. 29213) was cultured in LB medium (tryptone 10g/L, yeast extract 5g/L, sodium chloride 10g/L) to logarithmic phase, and the cell concentration was adjusted to 106 cells/L with LB medium to obtain a Staphylococcus aureus-containing bacterial solution.

0.5mL of each of the oral sprays (prepared by the method of example 6) according to example 3 and comparative examples 1 to 3 was added to 10m of the bacterial solution, and the mixture was cultured at 37 ℃ for 6 hours to obtain bacteriostatic samples.

The number of viable bacteria was measured by plate counting on the bacteriostatic samples according to the method (staphylococcus aureus test in food microbiology test) specified in GB4789.10-2010, and the results are shown in table 1:

TABLE 1

As can be seen from the above table 1, the oral spray of the invention has very strong antibacterial and antiviral properties, and has great popularization and application values.

Claims (10)

1. An antiviral noni SOD oral cavity spray is characterized by being prepared from the following substances in parts by weight:

7-10 parts of noni fruit extract micro powder, 5-8 parts of asarum, 3-6 parts of andrographis paniculata, 2-5 parts of platycodon grandiflorum, 1-4 parts of mint, 5-7 parts of liquorice, 2-6 parts of dandelion, 1-3 parts of scutellaria baicalensis, 1-3 parts of coptis chinensis and 900-1000 parts of water.

2. The antiviral noni SOD oral spray as claimed in claim 1, which is prepared from the following materials in parts by weight:

9 parts of noni fruit extract micro powder, 7 parts of asarum, 5 parts of common andrographis herb, 4 parts of platycodon grandiflorum, 3 parts of mint, 6 parts of liquorice, 4 parts of dandelion, 2 parts of scutellaria baicalensis, 2 parts of coptis chinensis and 960 parts of water.

3. The antiviral noni SOD oral spray according to claim 1 or 2, wherein the preparation method of the noni extract micropowder comprises the steps of:

1) washing noni fruits, soaking in ice water for 40-50 min, and taking out for later use;

2) drying the noni fruits treated in the step 1), and crushing the noni fruits to obtain crushed materials for later use;

3) immersing the crushed material obtained in the step 2) into an extracting solution, performing ultrasonic leaching treatment for 10-14 h, and performing suction filtration to obtain a suction filtration solution for later use;

4) carrying out high-speed centrifugation treatment on the suction filtration liquid obtained in the step 3), and removing centrifugal precipitates to obtain a centrifugal liquid for later use;

5) sequentially carrying out rough filtration and ultrafiltration treatment on the centrifugate obtained in the step 4) to obtain a fine filtrate for later use;

6) freeze-drying the fine filtrate obtained in the step 5) to obtain the noni fruit extract micro powder.

4. The antiviral noni SOD oral spray as claimed in claim 3, wherein the drying temperature in step 2) is not higher than 50 ℃; the particle size of the crushed materials is 150 meshes.

5. The antiviral noni SOD oral spray as claimed in claim 3, wherein the extract in step 3) is composed of the following substances in parts by weight: 2-2.5 parts of chitosan, 0.5-1.5 parts of tocopherol and 70-75 parts of edible alcohol; the concentration of the edible alcohol is 60%; the using amount of the extracting solution is 9-10 times of the total mass of the crushed materials; and the ultrasonic frequency is controlled to be 500-600 kHz during ultrasonic leaching treatment.

6. The antiviral noni SOD oral spray as claimed in claim 3, wherein the rotation speed of centrifugation is controlled to be 6000-6500 rpm in the high speed centrifugation treatment in step 4).

7. The anti-viral noni SOD oral spray as claimed in claim 3, wherein the pore size of the filter used in the rough filtration in step 5) is 15 μm, and the pore size of the filter used in the ultrafiltration is 0.2 μm.

8. The antiviral noni SOD oral spray according to claim 3, wherein the freezing temperature of the freeze-drying treatment in step 6) is from-30 to-35 ℃.

9. The preparation method of the antiviral noni SOD oral spray is characterized by comprising the following steps:

(1) firstly, mixing asarum, common andrographis herb, platycodon grandiflorum, mint, liquorice, dandelion, scutellaria baicalensis and coptis chinensis together, then putting into a pot containing wheat bran, continuously stir-frying for 50-55 min, taking out after completion, and removing redundant wheat bran to obtain a bran-fried medicine for later use;

(2) crushing the bran-fried medicine obtained in the step (1), then putting the crushed bran-fried medicine into clear water with the weight 5-7 times of the total weight of the bran-fried medicine, boiling the clear water for 1.5-2 hours, filtering the boiled clear water, and then concentrating the filtrate to 1/10 of the original volume for later use;

(3) and (3) mixing the noni fruit extract micro powder, the concentrated solution prepared in the step (2) and water, and fully and uniformly stirring to obtain the finished product of the oral spray.

10. The method for preparing anti-viral noni SOD oral spray according to claim 9, wherein the temperature in the pan is controlled to be 110-120 ℃ during the stir-frying process in step (1).