CN111662814A - Medical science inspection is with living fungus cultivation verifying attachment - Google Patents
Medical science inspection is with living fungus cultivation verifying attachment Download PDFInfo
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- CN111662814A CN111662814A CN202010492069.6A CN202010492069A CN111662814A CN 111662814 A CN111662814 A CN 111662814A CN 202010492069 A CN202010492069 A CN 202010492069A CN 111662814 A CN111662814 A CN 111662814A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/46—Means for fastening
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/50—Means for positioning or orientating the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
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Abstract
The invention discloses a viable bacteria cultivation and inspection device for medical inspection, which belongs to the technical field of medical instruments and comprises a shell with an opening at the top end, wherein a supporting table in a round table-shaped structure is arranged inside the shell, a culture dish is arranged at the top end of the supporting table, a temperature and humidity sensor is arranged on the inner wall of the right side of the shell, and an exhaust pipeline, an air inlet pipeline and an atomization pipeline which are communicated with the inside of the shell are sequentially arranged on the outer wall of the left side of the shell from top to bottom. When the cylinder is extended, the height of the support table is adjusted, so that when the support table is adjusted to be closer to the upper opening end of the shell, a worker can place a culture dish with strains on the support table conveniently, the contact area between the hand of the worker and the interior of the shell is reduced, and the culture environment in the shell is ensured.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a viable bacteria cultivation and inspection device for medical inspection.
Background
Bacterial culture is a technique for growing and reproducing bacteria by an artificial method; the bacteria are widely distributed in nature, have large quantity and various types, can benefit mankind and can also become the cause of disease; most bacteria can be cultured by an artificial method, namely, the bacteria are inoculated on a culture medium and are grown and propagated; the cultured bacteria are used for research, identification and application; with the continuous improvement of medical treatment level, people also have continuous deep research on various bacteria, so that the live bacteria cultivation and inspection device for medical inspection is innovated and designed, and plays an important role in the research on live bacteria.
Present most living fungus inspection usefulness cultivate verifying attachment because it needs manually to put the culture dish in the center department of equipment, leads to the culture dish to place the trouble, and the culture dish when placing, and people's hand will contact the inside of large tracts of land and equipment, leads to the environment in the equipment to receive the influence of hand bacterium easily, and causes the influence to the culture quality of living fungus, so, urgent needs develop a medical science inspection and cultivate verifying attachment with living fungus.
Disclosure of Invention
1. Technical problem to be solved
The invention aims to provide a viable bacteria cultivation and inspection device for medical inspection, which aims to solve the problems in the background technology.
2. Technical scheme
In order to solve the problems, the invention adopts the following technical scheme:
a viable bacteria cultivation and inspection device for medical inspection comprises a shell, a supporting table, a culture dish, a sealing cover plate, a temperature and humidity sensor, an exhaust pipeline, an air inlet pipeline, an atomization pipeline and a spray head, wherein the top end of the shell is open, the supporting table is arranged in the shell, the culture dish is placed at the top end of the supporting table, the temperature and humidity sensor is installed on the inner wall of the right side of the shell, the exhaust pipeline, the air inlet pipeline and the atomization pipeline which are communicated with the inside of the shell are sequentially installed on the outer wall of the left side of the shell from top to bottom, the spray head communicated with the atomization pipeline is installed on the inner wall of the left side of the shell, the sealing cover plate is hinged to the open end of the shell, transparent glass which is aligned with the supporting table is inlaid on the sealing cover plate, a twisting block is arranged at the top end, an upper independent accommodating cavity and a lower independent accommodating cavity are formed in the shell through a partition plate, a vertically arranged air cylinder is installed at the inner bottom of the shell, the top end of the air cylinder is fixedly connected with a slide rod which vertically extends upwards and the top end of the air cylinder is fixedly connected with the middle part of the bottom end of the supporting table, through holes for the slide rod to pass through are formed in the partition plate, an annular sleeving block is fixedly sleeved on the slide rod, four clamping mechanisms are arranged in the shell, the circle center surrounded by the four clamping mechanisms is located on the vertical central line of the supporting table, the clamping mechanisms are distributed around the supporting table at equal intervals, each clamping mechanism comprises a first hinge rod, the bottom end of which is hinged with the partition plate, a second hinge rod and a clamping block, the second hinge rod is hinged with the bottom end of the first hinge rod, the clamping block is fixed at the other end of the second hinge rod, and each second, and each third hinge rod is hinged with the corresponding second hinge rod, and each third hinge rod is hinged with the annular sleeving block.
Preferably, a limiting groove used for containing the bottom of the culture dish is formed in the supporting table, and a rubber pad is adhered to the inner bottom of the limiting groove.
Preferably, the inner side wall of the through hole is adhered with a sealing ring, and the inner wall surface of the sealing ring is tightly attached to the outer wall surface of the sliding rod.
Preferably, the end face of the inner side of the clamping block is of an arc-shaped structure, a rubber layer is adhered to the arc-shaped end face of the clamping block, and the shape of the rubber layer is the same as that of the arc-shaped end face.
Preferably, a spring fixedly connected with the inner side wall of the shell is fixed at the end part of the hinged shaft at the connecting end of each first hinged rod and each second hinged rod.
3. Advantageous effects
1. The number and the positions of the clamping mechanisms are arranged, so that each clamping mechanism is driven by the same air cylinder to realize opening or clamping, namely, four clamping mechanisms are in an open state when the air cylinder extends, and four clamping mechanisms are in a clamping state when the air cylinder contracts after extending, so that the periphery of the culture dish can be fixed in an extrusion mode through the contact between the four clamping mechanisms and the side wall of the culture dish when the four clamping mechanisms are clamped, the stability of the culture dish when cells are cultured is ensured, and the height of the supporting platform is adjusted when the air cylinder extends (at the moment, the four clamping mechanisms are in the open state), so that when the supporting platform is adjusted to be closer to the upper opening end of the shell, a worker can conveniently place the culture dish with strains on the supporting platform, the contact area between the hand of the worker and the interior of the shell is reduced, and the culture environment in the shell is ensured, the event is through the setting of above-mentioned clamping mechanism and cylinder linkage, makes this cultivation verifying attachment when making things convenient for the culture dish to place, has also carried out the assurance to the stability ability of culture dish during the cultivation.
2. When the cell culture device is used for culturing cells, the transparent glass and the support table are arranged at the position between the transparent glass and the support table, so that a worker can observe the culture condition of the cells in the culture dish from the transparent glass under the condition that the sealing cover plate is not opened, and can adjust the distance between the culture dish and the transparent glass on the support table through the extension of the air cylinder when the cultured cells need to be observed at a short distance, so that the worker can be in contact with the culture dish at a short distance so as to observe the cultured cells.
3. According to the invention, the arc-shaped structure of the clamping block effectively increases the attaching degree between the clamping block and the culture dish, improves the stability of the culture dish when the culture dish is fixed, and can buffer the culture dish and the clamping block when the culture dish is clamped and fixed through the deformation generated by the rubber layer, thereby avoiding the damage of the culture dish caused by large clamping force and achieving the protection effect.
4. According to the invention, the sealing ring is tightly attached to the sliding rod, so that a sealed cavity is defined between the cavity above the inner part of the shell and the sealing cover plate, and conditions are provided for culturing bacteria.
5. The limiting groove can limit the culture dish placed on the supporting table so as to facilitate the clamping mechanism to clamp and fix the culture dish.
Drawings
FIG. 1 is a schematic front view of the internal structure of the present invention;
FIG. 2 is a schematic top view of the support table;
FIG. 3 is a schematic top view of a slide bar;
FIG. 4 is a side view of the internal structure of the culture dish.
Reference numerals: 1-shell, 2-supporting table, 3-culture dish, 4-sealing cover plate, 5-transparent glass, 6-twisted block, 7-temperature and humidity sensor, 8-exhaust pipeline, 9-air inlet pipeline, 10-atomization pipeline, 11-partition plate, 12-air cylinder, 13-sliding rod, 14-through hole, 15-sealing ring, 16-first hinged rod, 17-second hinged rod, 18-third hinged rod, 19-clamping block, 20-rubber layer, 21-annular splicing block, 22-limiting groove, 23-rubber pad, 24-spray head and 25-spring.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Examples
As shown in fig. 1-4, a viable bacteria cultivation and inspection device for medical inspection comprises a housing 1 with an open top, a supporting platform 2, a culture dish 3, a sealing cover plate 4, a temperature and humidity sensor 7, an exhaust pipeline 8, an air inlet pipeline 9, an atomizing pipeline 10 and a nozzle 24, wherein the housing 1 is internally provided with the supporting platform 2 in a round platform-shaped structure, the culture dish 3 is placed on the top end of the supporting platform 2, the supporting platform 2 is provided with a limiting groove 22 for containing the bottom of the culture dish 3, the inner bottom of the limiting groove 22 is adhered with a rubber pad 23, the culture dish 3 placed on the supporting platform 2 can be limited by the limiting groove 22, so that the culture dish 3 can be clamped and fixed by a clamping mechanism, the temperature and humidity sensor 7 is installed on the inner wall of the right side of the housing 1, and the exhaust pipeline 8, the exhaust pipeline and the inner part of the housing 1 are, The air inlet pipeline 9 and the atomization pipeline 10, and a spray head 24 communicated with the atomization pipeline 10 is arranged on the left inner wall of the shell 1;
the open end of the shell 1 is hinged with a sealing cover plate 4, transparent glass 5 which is aligned with the supporting table 2 is inlaid on the sealing cover plate 4, a torsion block 6 is arranged at the top end of the sealing cover plate 4, a partition plate 11 which is positioned below the supporting table 2 is arranged in the shell 1, an upper independent accommodating chamber and a lower independent accommodating chamber are formed in the shell 1 through the partition plate 11, a vertically arranged air cylinder 12 is arranged at the inner bottom of the shell 1, a slide rod 13 which extends vertically upwards and is fixedly connected with the top end of the air cylinder 12 and the middle part of the bottom end of the supporting table 2 is fixedly connected with the top end of the air cylinder 12, a through hole 14 for the slide rod 13 to pass through is formed in the partition plate 11, a sealing ring 15 is adhered on the inner side wall of the through hole 14, the inner wall surface of the sealing ring 15 is tightly adhered to the outer wall surface of the slide rod 13, and, providing conditions for the culture of the bacteria;
an annular sleeving block 21 is fixedly sleeved on the sliding rod 13, four clamping mechanisms are arranged inside the shell 1, the circle center surrounded by the four clamping mechanisms is located on the vertical central line of the supporting platform 2, the clamping mechanisms are distributed around the supporting platform 2 at equal intervals, each clamping mechanism comprises a first hinge rod 16, the bottom end of which is hinged with the partition plate 11, a second hinge rod 17, the bottom end of which is hinged with the bottom end of the first hinge rod 16, and a clamping block 19 fixed on the other end of the second hinge rod 17, the inner side end face of the clamping block 19 is of an arc-shaped structure, a rubber layer 20 is adhered on the arc-shaped end face of the clamping block 19, the shape of the rubber layer 20 is the same as that of the arc-shaped end face, the arrangement of the arc-shaped structure of the clamping block 19 effectively increases the adhering degree between the clamping block 19 and the culture dish 3, the stability of the culture dish 3 when being fixed is improved, and deformation is, can be to being cushioned between the culture dish 3 when being pressed from both sides tightly fixed and the tight piece 19 of clamp, avoid the great damage that causes the culture dish 3 of clamp force, reach the safeguard effect, every second hinge pole 17 all is connected with annular cover joint piece 21 through third hinge pole 18, and every third hinge pole 18 all is connected with the articulated second hinge pole 17 that corresponds, and every third hinge pole 18 all is connected with annular cover joint piece 21 is articulated, the tip of articulated shaft all is fixed with the spring 25 with 1 inside wall fixed connection of casing on every first hinge pole 16 and the second hinge pole 17 connected end, have the ability that elasticity resets through spring 25, supplementary articulated hinge pole each other resets.
The live bacteria cultivation and inspection device for medical inspection has the specific application process that: when the device is used, the support platform 2 is moved to a position close to the open end on the shell 1 through the extension of the air cylinder 12, at the moment, the four clamping mechanisms are in an open state, the culture dish 3 filled with strains is placed in the limiting groove 22 on the support platform 2, the support platform 2 is contracted to the middle part of the upper containing cavity in the shell 1 through the contraction of the air cylinder 12, so that the cells can absorb nutrient substances in the upper containing cavity, in addition, when the air cylinder 12 is contracted, the downward pulling force of the second hinge rod 17 can be realized through the third hinge rod 18, the top end of the second hinge rod 17 is in the condition that the second hinge rod is hinged with the first hinge rod 16, when the cells are cultured, the temperature and the humidity in the upper containing cavity can be detected in real time through the temperature and humidity sensor 7, when the temperature and the humidity in the upper containing cavity exceed the set threshold values, the air can be exchanged through the exhaust pipeline 8, when the humidity in the upper chamber is low, the atomized liquid can be sprayed into the upper chamber through the nozzle 24 by the atomizing pipeline 10 connected with the external atomizer;
when the culture dish 3 is clamped and fixed, the attaching degree between the clamping block 19 and the culture dish 3 can be increased through the arrangement of the arc-shaped structure of the clamping block 19, the stability of the culture dish 3 during fixing is improved, and the culture dish 3 and the clamping block 19 are buffered through the deformation generated by the rubber layer 20 when being clamped and fixed, so that the culture dish 3 is prevented from being damaged due to large clamping force, and the protection effect is achieved;
when the cell is cultivateed a period of time and needs to be observed, the setting of position between accessible transparent glass 5 and the brace table 2, make the staff can be under the condition that sealed apron 4 does not open, the cultivation condition of the inside cell of culture dish 3 can be observed from transparent glass 5, and when needing to carry out closely the observation to the cell of cultivateing, the extension of accessible cylinder 12, adjust the distance between culture dish 3 and the transparent glass 5 on the brace table 2, make the staff can closely contact with culture dish 3, so that just be in the observation of cultivateing the cell, this kind just can detect the cell being cultivateed under the condition of not opening sealed apron 4, avoided current need open sealed apron 4 and taken out culture dish 3, and the pollution that causes the cell, just so accomplished this medical science inspection and used live bacteria cultivation verifying attachment's use process.
It should be understood by those skilled in the art that the above embodiments are only for illustrating the present invention and are not to be used as a limitation of the present invention, and that changes and modifications to the above embodiments are within the scope of the claims of the present invention as long as they are within the spirit and scope of the present invention.
Claims (5)
1. The utility model provides a medical science inspection is with living fungus cultivation verifying attachment, includes the uncovered casing in top (1), brace table (2), culture dish (3), sealed apron (4), temperature and humidity inductor (7), exhaust duct (8), admission line (9), atomization pipeline (10) and shower nozzle (24), the inside of casing (1) is equipped with brace table (2) that is round platform column structure, and the top of brace table (2) has placed culture dish (3), install temperature and humidity inductor (7) on the right side inner wall of casing (1), and from last to installing exhaust duct (8), admission line (9) and the atomization pipeline (10) that communicate with casing (1) inside in proper order down on the left side outer wall of casing (1) to install shower nozzle (24) with atomization pipeline (10) intercommunication on the left side inner wall of casing (1), the open end of casing (1) articulates and is connected with sealed apron (4), the novel glass sealing device is characterized in that transparent glass (5) which is aligned with the supporting table (2) is inlaid on the sealing cover plate (4), a twisting block (6) is arranged at the top end of the sealing cover plate (4), a partition plate (11) which is located below the supporting table (2) is arranged inside the shell (1), an upper independent cavity and a lower independent cavity are formed inside the shell (1) through the partition plate (11), a vertically arranged air cylinder (12) is installed at the inner bottom of the shell (1), a sliding rod (13) which vertically extends upwards and is fixedly connected with the top end of the air cylinder (12) and fixedly connected with the middle of the bottom end of the supporting table (2) is fixedly connected with the top end of the air cylinder (12), a through hole (14) for the sliding rod (13) to pass through is formed in the partition plate (11), an annular splicing block (21) is fixedly sleeved on the sliding rod (13, the circle center surrounded by the four clamping mechanisms is located on the vertical center line of the supporting table (2), the clamping mechanisms are distributed on the periphery of the supporting table (2) at equal intervals, each clamping mechanism comprises a first hinged rod (16) with the bottom end hinged to the partition plate (11), a second hinged rod (17) hinged to the bottom end of the first hinged rod (16) and a clamping block (19) fixed to the other end of the second hinged rod (17), each second hinged rod (17) is connected with an annular splicing block (21) through a third hinged rod (18), each third hinged rod (18) is hinged to the corresponding second hinged rod (17), and each third hinged rod (18) is hinged to the annular splicing block (21).
2. The viable bacteria cultivation and inspection device for medical inspection according to claim 1, wherein the support platform (2) is provided with a limit groove (22) for accommodating the bottom of the culture dish (3), and a rubber pad (23) is adhered to the inner bottom of the limit groove (22).
3. The viable bacteria cultivation and inspection device for medical inspection according to claim 1, wherein a sealing ring (15) is adhered to the inner side wall of the through hole (14), and the inner wall surface of the sealing ring (15) is closely attached to the outer wall surface of the sliding rod (13).
4. The viable bacteria cultivation and inspection device for medical inspection according to claim 1, wherein the inner end face of the clamping block (19) is arranged in an arc-shaped structure, a rubber layer (20) is adhered to the arc-shaped end face of the clamping block (19), and the shape of the rubber layer (20) is the same as that of the arc-shaped end face.
5. A living bacteria cultivation and inspection device for medical inspection according to claim 1, wherein a spring (25) fixedly connected with the inner side wall of the housing (1) is fixed on the end of the hinged shaft at the connection end of each of the first hinged rod (16) and the second hinged rod (17).
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CN112899136A (en) * | 2021-01-29 | 2021-06-04 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Device convenient for culturing and observing tubercle bacillus |
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CN112608840A (en) * | 2020-12-16 | 2021-04-06 | 李娄 | Novel adjustable cell culture dish frame |
CN112608840B (en) * | 2020-12-16 | 2023-08-25 | 上海倍锦生物科技有限公司 | Novel adjustable cell culture dish frame |
CN112899136A (en) * | 2021-01-29 | 2021-06-04 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Device convenient for culturing and observing tubercle bacillus |
CN113575401A (en) * | 2021-07-13 | 2021-11-02 | 满芝玲 | Plant culture apparatus for biological medicine |
CN113736629A (en) * | 2021-09-09 | 2021-12-03 | 侯孟信 | Biological treatment cell molecule monitoring system |
CN114181813A (en) * | 2021-12-13 | 2022-03-15 | 巩义市疾病预防控制中心 | Integrated microorganism inspection device and use method thereof |
CN114181813B (en) * | 2021-12-13 | 2024-01-16 | 巩义市疾病预防控制中心 | Integrated microorganism inspection device and application method thereof |
CN115895836A (en) * | 2023-02-08 | 2023-04-04 | 诸城舜沃农业科技有限公司 | Bacterial colony incubator and use method thereof |
CN115895836B (en) * | 2023-02-08 | 2024-03-08 | 诸城舜沃农业科技有限公司 | Colony incubator and use method |
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