CN111647542A - Biocontrol agent for optimal fermentation of compound microbial flora and preparation method thereof - Google Patents
Biocontrol agent for optimal fermentation of compound microbial flora and preparation method thereof Download PDFInfo
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- 230000004151 fermentation Effects 0.000 title claims abstract description 46
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 230000000813 microbial effect Effects 0.000 title claims abstract description 30
- 239000012681 biocontrol agent Substances 0.000 title claims abstract description 26
- 150000001875 compounds Chemical class 0.000 title claims abstract description 17
- 239000000463 material Substances 0.000 claims abstract description 68
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 54
- 239000006041 probiotic Substances 0.000 claims abstract description 48
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- 239000002689 soil Substances 0.000 claims abstract description 22
- 230000000443 biocontrol Effects 0.000 claims abstract description 19
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- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 claims abstract description 15
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000012538 ammonium bicarbonate Nutrition 0.000 claims abstract description 15
- 239000001099 ammonium carbonate Substances 0.000 claims abstract description 15
- 239000004202 carbamide Substances 0.000 claims abstract description 15
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- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 15
- 229940099596 manganese sulfate Drugs 0.000 claims abstract description 15
- 235000007079 manganese sulphate Nutrition 0.000 claims abstract description 15
- 239000011702 manganese sulphate Substances 0.000 claims abstract description 15
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims abstract description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 15
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 15
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims abstract description 15
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 14
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- 239000002985 plastic film Substances 0.000 claims description 7
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
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- Pest Control & Pesticides (AREA)
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- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
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- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
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- Microbiology (AREA)
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- Biochemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
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Abstract
The invention discloses a biocontrol preparation for optimal fermentation of a compound microbial flora and a preparation method thereof, and particularly relates to a biological preparation technology, wherein the used main materials comprise the following raw materials in parts by weight: 5-10 parts of composite probiotics, 50-60 parts of solid culture base material, 2-3 parts of urea, 1.5-3 parts of monopotassium phosphate, 0.1-1 part of magnesium sulfate, 0.3-0.8 part of manganese sulfate, 0.2-0.4 part of ammonium bicarbonate, 0.5-1 part of edible glucose, 2-6 parts of lactose, 1-2 parts of white granulated sugar, 1-2 parts of citric acid and 1-2 parts of stachyose. According to the invention, the waste generated by agricultural activities is utilized to firstly culture and screen out the composite probiotic, the raw materials for preparing the biocontrol agent comprise edible glucose, lactose, white granulated sugar, citric acid and stachyose, the activity of the composite probiotic is improved, the healthy growth of the soil microbial flora structure is effectively promoted, the culture base material can be recycled to prepare the organic fertilizer, the organic fertilizer is green and environment-friendly, the utilization rate of the agricultural activities is improved, the product process is simple and efficient, and the organic fertilizer is suitable for mass production and popularization and application.
Description
Technical Field
The embodiment of the invention relates to the technical field of biological agents, and particularly relates to a biocontrol agent for optimal fermentation of a compound microbial flora and a preparation method thereof.
Background
Biological preparation is "immune biological preparation" in the medical industry, and refers to biological products processed by microorganisms (bacteria, rickettsia, virus, etc.) and their metabolites, animal toxins, blood or tissues of human or animals, etc. to prevent, treat and diagnose corresponding infectious diseases or other related diseases, and the imbalance of soil microflora is a main factor causing continuous cropping obstacle of soil all the year round. The plant continuous cropping changes the physical and chemical properties of soil, and is also influenced by root exudates of the same type of plants, so that the microbial community of the soil is selectively enriched, the number of the soil microorganisms is obviously changed, and the structure of the microbial community of the soil is obviously changed. With the annual increase of the continuous cropping age of the facility vegetables, the types and the number of bacteria, actinomycetes and beneficial fungi in soil microorganisms are gradually reduced, and the number of pathogenic fungi is increased year by year, so that the occurrence of crop diseases is aggravated. The biological method for preventing and controlling vegetable crop diseases begins in the 80 th 20 th century, and a new disease-resistant crop variety is cultivated by utilizing a technology combining genetic breeding and genetic engineering, so that the method is an effective method for overcoming continuous cropping obstacles of vegetables.
The prior art has the following defects: according to the existing production mode of the biocontrol preparation, the obtained composite probiotic groups are low in quantity, large-scale composite probiotics propagation is inconvenient, mold production and popularization and application are influenced, and meanwhile, the activity of the composite probiotics is low, so that the healthy growth of the composite probiotics in soil is influenced, and the use of the biocontrol preparation is influenced.
Disclosure of Invention
Therefore, the embodiment of the invention provides a biocontrol agent for optimal fermentation of a complex microbial flora and a preparation method thereof, the composite probiotic bacteria are cultured and screened out by utilizing wastes generated by agricultural activities, the composite probiotic bacteria are simple to culture, the biocontrol preparation raw materials comprise edible glucose, lactose, white granulated sugar, citric acid and stachyose, the nutrients are provided for the composite probiotic bacteria, the activity of the composite probiotic bacteria is improved, the healthy growth of the soil microbial flora structure is effectively promoted, the culture base materials can be recycled to prepare the organic fertilizer, the organic fertilizer is green and environment-friendly, the utilization rate of the agricultural activities is improved, the product process is simple and efficient, and the organic fertilizer is suitable for mass production and popularization and application, so as to solve the problems that the prior art is inconvenient for large-scale complex probiotics propagation due to lower quantity of the synthetic probiotics group, influences the mold production and popularization and application, meanwhile, the activity of the composite probiotics is low, and the problem caused by the influence on the healthy growth of the composite probiotics in the soil is solved.
In order to achieve the above object, the embodiments of the present invention provide the following technical solutions: a biocontrol agent for optimal fermentation of a compound microbial flora comprises: the used main materials comprise the following raw materials in parts by weight: 5-10 parts of composite probiotics, 50-60 parts of solid culture base material, 2-3 parts of urea, 1.5-3 parts of monopotassium phosphate, 0.1-1 part of magnesium sulfate, 0.3-0.8 part of manganese sulfate, 0.2-0.4 part of ammonium bicarbonate, 0.5-1 part of edible glucose, 2-6 parts of lactose, 1-2 parts of white granulated sugar, 1-2 parts of citric acid and 1-2 parts of stachyose.
Further, the solid culture medium material is prepared by mixing and matching plant straws, sawdust and plant ash, wherein the plant straws refer to straws of rice, wheat, sorghum or corn.
Furthermore, the composite probiotics is prepared by mixing 15-30% of lactobacillus acidophilus, 20-25% of bifidobacterium lactis, 10-15% of bifidobacterium longum and 30-55% of streptococcus thermophilus.
A preparation method of a biocontrol agent for optimal fermentation of a compound microbial flora comprises the following steps:
the specific operation mode is as follows:
the method comprises the following steps: crushing the solid culture base material, adding tap water, stirring with a stirrer to obtain biological flora culture base material with water content of 40-60%, heating to 121 deg.C, sterilizing for 20-30min, and cooling to room temperature;
step two: weighing corresponding parts of composite probiotics, stirring and mixing the composite probiotics with the biological flora culture base material in the step one to prepare a culture material, adjusting the pH to 6 by using a hydrochloric acid solution with the mass concentration of 9%, and naturally culturing and fermenting after barreling;
step three: performing solid-liquid separation on the compost subjected to barrel-packed fermentation culture in the step two, uniformly stirring and mixing the solid base material, the turfy soil and the phosphate fertilizer, and drying the mixture to prepare an organic fertilizer by using a granulator;
step four: purifying and filtering the liquid bacterial liquid separated in the third step, and storing the liquid bacterial liquid in a sterile container for later use;
step five: weighing urea, monopotassium phosphate, magnesium sulfate, manganese sulfate, ammonium bicarbonate, edible glucose, lactose, white granulated sugar, citric acid and stachyose in corresponding parts, uniformly stirring and mixing with the bacterial liquid purified in the step three to obtain a biocontrol preparation liquid, and subsequently bottling to obtain a biocontrol preparation finished product
Further, the compost filled into the barrel in the second step is turned over once every 3 to 5 days in the fermentation process.
Further, in the second step, a plastic film is used for sealing, standing and fermenting for 14-16 days in a sealed mode, the growth condition of the bacteria is sampled and detected, the pH value is measured, the total concentration of viable bacteria is detected, and the fermentation is stopped when the total concentration of the viable bacteria exceeds 10/ML and the pH value is 3.0.
Further, in the third step, the separated solid base material is centrifuged at the centrifugal force of 4000 Xg for 30 minutes and then dried by hot air at 40 ℃ for 10 hours, so that the water content is reduced to below 25 percent.
Further, stirring and mixing are carried out in the fifth step by adopting an ultrasonic oscillator, ultrasonic dispersion mixing stirring is carried out for 20-30min, the ultrasonic frequency is 25-35kHz, and the ultrasonic power is 100-200W.
The embodiment of the invention has the following advantages:
according to the invention, the waste generated by agricultural activities is utilized to firstly culture and screen out the composite probiotic bacteria consisting of lactobacillus acidophilus, bifidobacterium lactis, bifidobacterium longum and streptococcus thermophilus, the composite probiotic bacteria are simple to culture, the raw materials for preparing the biocontrol preparation comprise edible glucose, lactose, white granulated sugar, citric acid and stachyose, so that the nutrients are provided for the composite probiotic bacteria, the activity of the composite probiotic bacteria is improved, the healthy growth of the soil microbial flora structure is effectively promoted, the activity of the soil enzyme can also be improved, the culture base material for culturing the flora can be recycled to be prepared into the organic fertilizer, the environment-friendly effect is achieved, the agricultural activity utilization rate is improved, the product process is simple and efficient, and the method is suitable.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a biocontrol agent for optimal fermentation of a compound microbial flora comprises: the used main materials comprise the following raw materials in parts by weight: 5 parts of composite probiotics, 50 parts of solid culture base material, 2 parts of urea, 1.5 parts of monopotassium phosphate, 0.1 part of magnesium sulfate, 0.3 part of manganese sulfate, 0.2 part of ammonium bicarbonate, 0.5 part of edible glucose, 2 parts of lactose, 1 part of white granulated sugar, 1 part of citric acid and 1 part of stachyose.
Further, the solid culture medium material is prepared by mixing and matching plant straws, sawdust and plant ash, wherein the plant straws refer to straws of rice, wheat, sorghum or corn.
Furthermore, the composite probiotics is prepared by mixing 15-30% of lactobacillus acidophilus, 20-25% of bifidobacterium lactis, 10-15% of bifidobacterium longum and 30-55% of streptococcus thermophilus.
A preparation method of a biocontrol agent for optimal fermentation of a compound microbial flora comprises the following steps:
the specific operation mode is as follows:
the method comprises the following steps: crushing the solid culture base material, adding tap water, stirring with a stirrer to obtain biological flora culture base material with water content of 40-60%, heating to 121 deg.C, sterilizing for 20min, and cooling to room temperature;
step two: weighing corresponding parts of composite probiotics, stirring and mixing the composite probiotics with the biological flora culture base material in the step one to prepare a culture material, adjusting the pH to 6 by using a hydrochloric acid solution with the mass concentration of 9%, and naturally culturing and fermenting after barreling;
step three: performing solid-liquid separation on the compost subjected to barrel-packed fermentation culture in the step two, uniformly stirring and mixing the solid base material, the turfy soil and the phosphate fertilizer, and drying the mixture to prepare an organic fertilizer by using a granulator;
step four: purifying and filtering the liquid bacterial liquid separated in the third step, and storing the liquid bacterial liquid in a sterile container for later use;
step five: weighing urea, monopotassium phosphate, magnesium sulfate, manganese sulfate, ammonium bicarbonate, edible glucose, lactose, white granulated sugar, citric acid and stachyose in corresponding parts, uniformly stirring and mixing with the bacterial liquid purified in the step three to obtain a biocontrol preparation liquid, and subsequently bottling to obtain a biocontrol preparation finished product
Further, the compost filled into the barrel in the second step is turned and piled once every 3 days in the fermentation process.
Further, in the second step, a plastic film is used for sealing, standing and fermenting for 14 days in a sealed mode, the growth condition of the bacteria is sampled and detected, the pH value is measured, the total concentration of the viable bacteria is detected, and the fermentation is stopped when the total concentration of the viable bacteria exceeds 10/ML and the pH value is 3.0.
Further, in the third step, the separated solid base material is centrifuged at the centrifugal force of 4000 Xg for 30 minutes and then dried by hot air at 40 ℃ for 10 hours, so that the water content is reduced to below 25 percent.
Further, stirring and mixing are carried out in the fifth step by adopting an ultrasonic oscillator, ultrasonic dispersion mixing and stirring are carried out for 20min, the ultrasonic frequency is 25-35kHz, and the ultrasonic power is 100-200W.
Example 2:
a biocontrol agent for optimal fermentation of a compound microbial flora comprises: the used main materials comprise the following raw materials in parts by weight: 5 parts of composite probiotics, 50 parts of solid culture base material, 2 parts of urea, 1.5 parts of monopotassium phosphate, 0.1 part of magnesium sulfate, 0.3 part of manganese sulfate, 0.2 part of ammonium bicarbonate, 0.5 part of edible glucose, 2 parts of lactose, 1 part of white granulated sugar, 1 part of citric acid and 1 part of stachyose.
Further, the solid culture medium material is prepared by mixing and matching plant straws, sawdust and plant ash, wherein the plant straws refer to straws of rice, wheat, sorghum or corn.
Furthermore, the composite probiotics is prepared by mixing 15-30% of lactobacillus acidophilus, 20-25% of bifidobacterium lactis, 10-15% of bifidobacterium longum and 30-55% of streptococcus thermophilus.
A preparation method of a biocontrol agent for optimal fermentation of a compound microbial flora comprises the following steps:
the specific operation mode is as follows:
the method comprises the following steps: crushing the solid culture base material, adding tap water, stirring with a stirrer to obtain biological flora culture base material with water content of 40-60%, heating to 121 deg.C, sterilizing for 30min, and cooling to room temperature;
step two: weighing corresponding parts of composite probiotics, stirring and mixing the composite probiotics with the biological flora culture base material in the step one to prepare a culture material, adjusting the pH to 6 by using a hydrochloric acid solution with the mass concentration of 9%, and naturally culturing and fermenting after barreling;
step three: performing solid-liquid separation on the compost subjected to barrel-packed fermentation culture in the step two, uniformly stirring and mixing the solid base material, the turfy soil and the phosphate fertilizer, and drying the mixture to prepare an organic fertilizer by using a granulator;
step four: purifying and filtering the liquid bacterial liquid separated in the third step, and storing the liquid bacterial liquid in a sterile container for later use;
step five: weighing urea, monopotassium phosphate, magnesium sulfate, manganese sulfate, ammonium bicarbonate, edible glucose, lactose, white granulated sugar, citric acid and stachyose in corresponding parts, uniformly stirring and mixing with the bacterial liquid purified in the step three to obtain a biocontrol preparation liquid, and subsequently bottling to obtain a biocontrol preparation finished product
Further, the compost filled into the barrel in the second step is turned and piled once every 5 days in the fermentation process.
Further, in the second step, a plastic film is used for sealing, standing and fermenting for 16 days in a sealed mode, the growth condition of the bacteria is sampled and detected, the pH value is measured, the total concentration of the viable bacteria is detected, and the fermentation is stopped when the total concentration of the viable bacteria exceeds 10/ML and the pH value is 3.0.
Further, in the third step, the separated solid base material is centrifuged at the centrifugal force of 4000 Xg for 30 minutes and then dried by hot air at 40 ℃ for 10 hours, so that the water content is reduced to below 25 percent.
Further, stirring and mixing are carried out in the fifth step by adopting an ultrasonic oscillator, ultrasonic dispersion mixing stirring is carried out for 30min, the ultrasonic frequency is 25-35kHz, and the ultrasonic power is 100-200W.
Example 3:
a biocontrol agent for optimal fermentation of a compound microbial flora comprises: the used main materials comprise the following raw materials in parts by weight: 7 parts of composite probiotics, 55 parts of solid culture base material, 2.5 parts of urea, 2 parts of monopotassium phosphate, 0.5 part of magnesium sulfate, 0.5 part of manganese sulfate, 0.3 part of ammonium bicarbonate, 0.7 part of edible glucose, 4 parts of lactose, 1.5 parts of white granulated sugar, 1.5 parts of citric acid and 1.5 parts of stachyose.
Further, the solid culture medium material is prepared by mixing and matching plant straws, sawdust and plant ash, wherein the plant straws refer to straws of rice, wheat, sorghum or corn.
Furthermore, the composite probiotics is prepared by mixing 15-30% of lactobacillus acidophilus, 20-25% of bifidobacterium lactis, 10-15% of bifidobacterium longum and 30-55% of streptococcus thermophilus.
A preparation method of a biocontrol agent for optimal fermentation of a compound microbial flora comprises the following steps:
the specific operation mode is as follows:
the method comprises the following steps: crushing the solid culture base material, adding tap water, stirring with a stirrer to obtain biological flora culture base material with water content of 40-60%, heating to 121 deg.C, sterilizing for 25min, and cooling to room temperature;
step two: weighing corresponding parts of composite probiotics, stirring and mixing the composite probiotics with the biological flora culture base material in the step one to prepare a culture material, adjusting the pH to 6 by using a hydrochloric acid solution with the mass concentration of 9%, and naturally culturing and fermenting after barreling;
step three: performing solid-liquid separation on the compost subjected to barrel-packed fermentation culture in the step two, uniformly stirring and mixing the solid base material, the turfy soil and the phosphate fertilizer, and drying the mixture to prepare an organic fertilizer by using a granulator;
step four: purifying and filtering the liquid bacterial liquid separated in the third step, and storing the liquid bacterial liquid in a sterile container for later use;
step five: weighing urea, monopotassium phosphate, magnesium sulfate, manganese sulfate, ammonium bicarbonate, edible glucose, lactose, white granulated sugar, citric acid and stachyose in corresponding parts, uniformly stirring and mixing with the bacterial liquid purified in the step three to obtain a biocontrol preparation liquid, and subsequently bottling to obtain a biocontrol preparation finished product
Further, the compost filled into the barrel in the second step is turned and piled once every 4 days in the fermentation process.
Further, in the second step, a plastic film is used for sealing, standing and fermenting for 15 days in a sealed mode, the growth condition of the bacteria is sampled and detected, the pH value is measured, the total concentration of the viable bacteria is detected, and the fermentation is stopped when the total concentration of the viable bacteria exceeds 10/ML and the pH value is 3.0.
Further, in the third step, the separated solid base material is centrifuged at the centrifugal force of 4000 Xg for 30 minutes and then dried by hot air at 40 ℃ for 10 hours, so that the water content is reduced to below 25 percent.
Further, stirring and mixing are carried out in the fifth step by adopting an ultrasonic oscillator, ultrasonic dispersion mixing and stirring are carried out for 25min, the ultrasonic frequency is 25-35kHz, and the ultrasonic power is 100-200W.
Example 4:
a biocontrol agent for optimal fermentation of a compound microbial flora comprises: the used main materials comprise the following raw materials in parts by weight: 10 parts of composite probiotics, 60 parts of solid culture base material, 3 parts of urea, 3 parts of monopotassium phosphate, 1 part of magnesium sulfate, 0.8 part of manganese sulfate, 0.4 part of ammonium bicarbonate, 1 part of edible glucose, 6 parts of lactose, 2 parts of white granulated sugar, 2 parts of citric acid and 2 parts of stachyose.
Further, the solid culture medium material is prepared by mixing and matching plant straws, sawdust and plant ash, wherein the plant straws refer to straws of rice, wheat, sorghum or corn.
Furthermore, the composite probiotics is prepared by mixing 15-30% of lactobacillus acidophilus, 20-25% of bifidobacterium lactis, 10-15% of bifidobacterium longum and 30-55% of streptococcus thermophilus.
A preparation method of a biocontrol agent for optimal fermentation of a compound microbial flora comprises the following steps:
the specific operation mode is as follows:
the method comprises the following steps: crushing the solid culture base material, adding tap water, stirring with a stirrer to obtain biological flora culture base material with water content of 40-60%, heating to 121 deg.C, sterilizing for 20min, and cooling to room temperature;
step two: weighing corresponding parts of composite probiotics, stirring and mixing the composite probiotics with the biological flora culture base material in the step one to prepare a culture material, adjusting the pH to 6 by using a hydrochloric acid solution with the mass concentration of 9%, and naturally culturing and fermenting after barreling;
step three: performing solid-liquid separation on the compost subjected to barrel-packed fermentation culture in the step two, uniformly stirring and mixing the solid base material, the turfy soil and the phosphate fertilizer, and drying the mixture to prepare an organic fertilizer by using a granulator;
step four: purifying and filtering the liquid bacterial liquid separated in the third step, and storing the liquid bacterial liquid in a sterile container for later use;
step five: weighing urea, monopotassium phosphate, magnesium sulfate, manganese sulfate, ammonium bicarbonate, edible glucose, lactose, white granulated sugar, citric acid and stachyose in corresponding parts, uniformly stirring and mixing with the bacterial liquid purified in the step three to obtain a biocontrol preparation liquid, and subsequently bottling to obtain a biocontrol preparation finished product
Further, the compost filled into the barrel in the second step is turned and piled once every 3 days in the fermentation process.
Further, in the second step, a plastic film is used for sealing, standing and fermenting for 14 days in a sealed mode, the growth condition of the bacteria is sampled and detected, the pH value is measured, the total concentration of the viable bacteria is detected, and the fermentation is stopped when the total concentration of the viable bacteria exceeds 10/ML and the pH value is 3.0.
Further, in the third step, the separated solid base material is centrifuged at the centrifugal force of 4000 Xg for 30 minutes and then dried by hot air at 40 ℃ for 10 hours, so that the water content is reduced to below 25 percent.
Further, stirring and mixing are carried out in the fifth step by adopting an ultrasonic oscillator, ultrasonic dispersion mixing and stirring are carried out for 20min, the ultrasonic frequency is 25-35kHz, and the ultrasonic power is 100-200W.
Example 5:
a biocontrol agent for optimal fermentation of a compound microbial flora comprises: the used main materials comprise the following raw materials in parts by weight: 10 parts of composite probiotics, 60 parts of solid culture base material, 3 parts of urea, 3 parts of monopotassium phosphate, 1 part of magnesium sulfate, 0.8 part of manganese sulfate, 0.4 part of ammonium bicarbonate, 1 part of edible glucose, 6 parts of lactose, 2 parts of white granulated sugar, 2 parts of citric acid and 2 parts of stachyose.
Further, the solid culture medium material is prepared by mixing and matching plant straws, sawdust and plant ash, wherein the plant straws refer to straws of rice, wheat, sorghum or corn.
Furthermore, the composite probiotics is prepared by mixing 15-30% of lactobacillus acidophilus, 20-25% of bifidobacterium lactis, 10-15% of bifidobacterium longum and 30-55% of streptococcus thermophilus.
A preparation method of a biocontrol agent for optimal fermentation of a compound microbial flora comprises the following steps:
the specific operation mode is as follows:
the method comprises the following steps: crushing the solid culture base material, adding tap water, stirring with a stirrer to obtain biological flora culture base material with water content of 40-60%, heating to 121 deg.C, sterilizing for 30min, and cooling to room temperature;
step two: weighing corresponding parts of composite probiotics, stirring and mixing the composite probiotics with the biological flora culture base material in the step one to prepare a culture material, adjusting the pH to 6 by using a hydrochloric acid solution with the mass concentration of 9%, and naturally culturing and fermenting after barreling;
step three: performing solid-liquid separation on the compost subjected to barrel-packed fermentation culture in the step two, uniformly stirring and mixing the solid base material, the turfy soil and the phosphate fertilizer, and drying the mixture to prepare an organic fertilizer by using a granulator;
step four: purifying and filtering the liquid bacterial liquid separated in the third step, and storing the liquid bacterial liquid in a sterile container for later use;
step five: weighing urea, monopotassium phosphate, magnesium sulfate, manganese sulfate, ammonium bicarbonate, edible glucose, lactose, white granulated sugar, citric acid and stachyose in corresponding parts, uniformly stirring and mixing with the bacterial liquid purified in the step three to obtain a biocontrol preparation liquid, and subsequently bottling to obtain a biocontrol preparation finished product
Further, the compost filled into the barrel in the second step is turned and piled once every 5 days in the fermentation process.
Further, in the second step, a plastic film is used for sealing, standing and fermenting for 16 days in a sealed mode, the growth condition of the bacteria is sampled and detected, the pH value is measured, the total concentration of the viable bacteria is detected, and the fermentation is stopped when the total concentration of the viable bacteria exceeds 10/ML and the pH value is 3.0.
Further, in the third step, the separated solid base material is centrifuged at the centrifugal force of 4000 Xg for 30 minutes and then dried by hot air at 40 ℃ for 10 hours, so that the water content is reduced to below 25 percent.
Further, stirring and mixing are carried out in the fifth step by adopting an ultrasonic oscillator, ultrasonic dispersion mixing stirring is carried out for 30min, the ultrasonic frequency is 25-35kHz, and the ultrasonic power is 100-200W.
The biocontrol agents prepared in the above examples 1-5 are respectively selected from the same corn field, 180 corns with the same growth vigor in the corn field are selected, 30 corns are respectively allocated in the five examples and the comparative examples to carry out spraying experiments, the spraying is carried out in sequence every five days, and after 1 month of use, the following data are obtained:
as can be seen from the above table, the raw materials in example 3 have a moderate mixing ratio, so that the composite probiotics can grow healthily in the soil, optimize the soil, promote the growth of crops, improve the yield of crops, reduce the harm of harmful bacteria to crops, and reduce the occurrence of diseases.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. A biocontrol agent for optimal fermentation of a compound microbial flora is characterized in that: the used main materials comprise the following raw materials in parts by weight: 5-10 parts of composite probiotics, 50-60 parts of solid culture base material, 2-3 parts of urea, 1.5-3 parts of monopotassium phosphate, 0.1-1 part of magnesium sulfate, 0.3-0.8 part of manganese sulfate, 0.2-0.4 part of ammonium bicarbonate, 0.5-1 part of edible glucose, 2-6 parts of lactose, 1-2 parts of white granulated sugar, 1-2 parts of citric acid and 1-2 parts of stachyose.
2. The biocontrol agent for optimal fermentation of complex microbial flora according to claim 1, wherein: the solid culture medium material is prepared by mixing and matching plant straws, sawdust and plant ash, wherein the plant straws refer to straws of rice, wheat, sorghum or corn.
3. The biocontrol agent for optimal fermentation of complex microbial flora according to claim 1, wherein: the composite probiotics is prepared by mixing and proportioning 15-30% of lactobacillus acidophilus, 20-25% of bifidobacterium lactis, 10-15% of bifidobacterium longum and 30-55% of streptococcus thermophilus.
4. A method for preparing a biocontrol agent for optimal fermentation of complex microbial flora according to any of claims 1-3, characterized in that:
the specific operation mode is as follows:
the method comprises the following steps: crushing the solid culture base material, adding tap water, stirring with a stirrer to obtain biological flora culture base material with water content of 40-60%, heating to 121 deg.C, sterilizing for 20-30min, and cooling to room temperature;
step two: weighing corresponding parts of composite probiotics, stirring and mixing the composite probiotics with the biological flora culture base material in the step one to prepare a culture material, adjusting the pH to 6 by using a hydrochloric acid solution with the mass concentration of 9%, and naturally culturing and fermenting after barreling;
step three: performing solid-liquid separation on the compost subjected to barrel-packed fermentation culture in the step two, uniformly stirring and mixing the solid base material, the turfy soil and the phosphate fertilizer, and drying the mixture to prepare an organic fertilizer by using a granulator;
step four: purifying and filtering the liquid bacterial liquid separated in the third step, and storing the liquid bacterial liquid in a sterile container for later use;
step five: weighing urea, monopotassium phosphate, magnesium sulfate, manganese sulfate, ammonium bicarbonate, edible glucose, lactose, white granulated sugar, citric acid and stachyose in corresponding parts, uniformly stirring and mixing with the bacterial liquid purified in the step three to obtain a biocontrol preparation liquid, and subsequently bottling to obtain a biocontrol preparation finished product.
5. The method for preparing a biocontrol agent for optimal fermentation of complex microbial flora as claimed in claim 4, wherein: and turning the composts filled into the barrels in the second step once every 3 to 5 days in the fermentation process.
6. The method for preparing a biocontrol agent for optimal fermentation of complex microbial flora as claimed in claim 4, wherein: and in the second step, a plastic film is used for sealing, standing and fermenting for 14-16 days in a sealed mode, the growth condition of the bacteria is sampled and detected, the pH value is measured, the total concentration of the viable bacteria is detected, and the fermentation is stopped when the total concentration of the viable bacteria exceeds 10/ML and the pH value is 3.0.
7. The method for preparing a biocontrol agent for optimal fermentation of complex microbial flora as claimed in claim 4, wherein: in the third step, the separated solid base material is centrifuged for 30 minutes under the centrifugal force of 4000 Xg and then dried by hot air at 40 ℃ for 10 hours, so that the water content is reduced to below 25 percent.
8. The method for preparing a biocontrol agent for optimal fermentation of complex microbial flora as claimed in claim 4, wherein: and step five, stirring and mixing by using an ultrasonic oscillator, wherein ultrasonic dispersion mixing stirring is performed for 20-30min, the ultrasonic frequency is 25-35kHz, and the ultrasonic power is 100-200W.
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