CN1116419C - Enzymolysis process for preparing functional oligoxylose - Google Patents
Enzymolysis process for preparing functional oligoxylose Download PDFInfo
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- CN1116419C CN1116419C CN99126547A CN99126547A CN1116419C CN 1116419 C CN1116419 C CN 1116419C CN 99126547 A CN99126547 A CN 99126547A CN 99126547 A CN99126547 A CN 99126547A CN 1116419 C CN1116419 C CN 1116419C
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Abstract
The present invention discloses a method for preparing oligomer xylose used as functional food and a food additive by an enzymolysis method. The method comprises the following steps: corn cob, corn straw, rice straw, ec., which are used as raw materials are made by selecting, drying, crushing and sieving; the raw materials are soaked by water and dewatered; at the room temperature and high temperature, alkali is agitated and leached to collect filter liquor; the filter liquor is neutralized by acid to present subacidity; enzyme powder or enzyme preparation is added in hemicellulose solution which is obtained; after enzymic hydrolysate is processed by decoloring, ion exchange and concentration, a mixture having the main component of oligomer dixylose, or crystals of the oligomer dixylose is obtained. Functional oligomer xylose has wide application prospects on food products, fodder, health care and plant protection.
Description
Technical field
The present invention relates to a kind of preparation method of functional oligose, specifically is the production technique that enzyme process prepares functional low polyxylose.
Prior art
Oligose claims oligosaccharides again, is to be formed by 2-10 monose molecule dehydrating condensation, owing to the formation glycan molecule is different with combination all titles is arranged, and its physico-chemical property and physiological function are also had nothing in common with each other.The called function oligose is meant the people, animals and plants have the oligose of special physiological effect, sugariness generally has only about the 30%-50% of sucrose, because of its indigestibility, lower calorific value, be difficult for causing blood sugar with it as sweeting agent, Regular Insulin raises, can not cause fat yet, some oligose is absorbed by human consumption because of difficulty, so can directly go into large intestine by small intestine, and play the effect of food fibre, and it can only be for the bifidus utilization can not be utilized by the enteron aisle putrefactive bacterium, thereby (this class oligose comprises isomaltose to have whole intestines effect, xylo-oligosaccharide, oligofructose or the like).Some decompose the effect that the oligose that obtains has plant growth regulation and inducing plant generation antibiotics by plant or fungal cell wall polysaccharide, and xylo-oligosaccharide, oligomeric beta-glucan etc. are wherein arranged.The specific function of oligose has caused the attention of academia, along with the discovery of various enzymes and the progress of isolation technique, the development of leap has been arranged for the research of oligose in the world, range of application is constantly opened up, and is that the Application and Development of the new functionalized oligose of background has become the new focus that biological technical field is studied with the high-tech.
Yet the current still bifidus factor effect of noting most of xylo-oligosaccharide.As everyone knows, bifidus is the normal bacterium of a kind of human intestinal, and baby due bifidus bacillus promptly occurred on 3rd or four, and had comparative advantage.Along with age increase bifidus bacillus reduces gradually, and putrefactive bacteriums such as perfringens fusobacterium, intestinal bacteria roll up.To old age, enteron aisle is full of putrefactive bacterium, and bifidus bacillus almost disappears.Putrefactive bacterium decomposes food composition in enteron aisle, produce poisonous variation sources such as ammonia, amine, hydrogen sulfide, skatole, indoles, phenols and nitrosamine, human body absorbs these toxin for a long time, can promote aging, bring out cancer, cause diseases such as arteriosclerosis, liver obstacle.Bifidus bacillus then plays an important role to the defendance HUMAN HEALTH: 1. suppress the breeding of harmful intestinal tract microorganism, decompose carbohydrate and produce organic acid, keep the intestinal microflora balance, promote intestines peristalsis, help preventing constipation: 2. not corrupt protein, do not generate objectionable impurities, promote protein digestion to absorb; 3. synthetic vitamin B group, and promote some inorganic salt is utilized; 4. produce some material and can strengthen the immunizing power that improves body; 5. decompose carcinogenic substances prevents and suppresses the generation of tumour, also can prevent the savings of body inner cholesterol.
Summary of the invention
The object of the present invention is to provide a kind of preparation method who makes functional foodstuff and foodstuff additive based on oligomeric two wood sugars.
For achieving the above object, the technical solution used in the present invention:
A kind of enzyme process prepares the production technique of functional low polyxylose, and it may further comprise the steps:
A. corn cob, straw, bagasse, wheat straw powder, straw etc. carried out drying, pulverize, sieving makes raw material;
B. above-mentioned raw materials soaks, dewaters with clear water, uses under the alkali room temperature stirring and leaching again 15～30 hours, and stirring and leaching is 1～3 hour in the time of 70～90 ℃, removes solid substance, collects filtrate, and is neutralized to subacidity with acid buffer, obtains hemicellulose solution;
C. add the zymin hydrolyzed hemicellulose behind enzyme powder or the purifying in above-mentioned hemicellulose solution, it is main mixture that the hydrolyzed solution of enzyme gets oligomeric xylo-bioses through decolouring, ion-exchange, process such as concentrate again.
D. above-mentioned zymin mainly is zytase (EC3.2.1.8), it can be made by solid-state or liquid state fermentation method respectively by microorganism, and the zytase of Chan Shenging can be prepared into partially purified zymin or the pure enzyme behind sephadex G-100 and DEAE-sephadex-A50 purifying by fermentation.
The also available flow process as shown in Figure 1 of above-mentioned production technique is represented;
With corn cob, corn stalk, straw etc. as raw material through screening, dry, pulverize, sieving makes raw material, above-mentioned raw materials water-soaked, dehydration, with alkali stirring and leaching under room temperature and high temperature, remove solid substance, collect filtrate, be neutralized to subacidity, get hemicellulose solution with acid, in the solution that makes, add enzyme powder or zymin hydrolyzed hemicellulose, the hydrolyzed solution of enzyme again through decolouring, ion-exchange, after concentrating oligomeric two wood sugars be main mixture.
Bacterium in the healthy human body digestive tube has kind more than 100, and its quantity reaches more than 1,000 hundred million.Wherein bifidus bacillus takes advantage in enteron aisle, and bifidus bacillus how much in the inspection defecation, just can how judge HUMAN HEALTH.The flora structure is near the young adult in the long lived elder enteron aisle, and this is confirmed by Chinese scholars.Therefore if can adjust the intestinal microflora structure, bifidus bacillus is had the advantage, undoubtedly to people's health and anti-ageing be useful, edible bifidus factor is a kind of strong supplementary means, xylo-oligosaccharide has proved to have good bifidobacterium factor functional.At present, the domestic patent that does not also retrieve the like product of xylo-oligosaccharide.Zymin among the present invention is by black mold production, and raw materials for production are edible agriculture organic waste materials, and to the human body safety non-toxic, the functional low polyxylose of being produced can be at food, feed, and health care and plant protection aspect all hold out broad prospects.
Summary of drawings
Fig. 1 is a process flow sheet of the present invention;
Fig. 2 is a concrete processing condition schema of the present invention.
Embodiment
The concrete technological process that provides below in conjunction with Fig. 2 describes in detail.
Raw material processing: the raw material in order to the production functional low polyxylose mainly is from agriculture organic waste materials, as corn cob, corn stalk, bagasse, wheat straw powder, wheat bran, straw, Pericarppium arachidis hypogaeae, jowar stalk etc., these raw materials all are rich in hemicellulose, and nontoxic to human body, it is safe and reliable to make functional low polyxylose by them.Raw material such as corn cob, straw at first should screen removes the composition that goes mouldy, and kept dry, and after the classified again pulverizing, it is standby to cross 360 mesh sieves.
The preparation of hemicellulose: above-mentioned raw materials is fully soaked with clear water, and centrifugal removal moisture after the swelling is again with the solid-to-liquid ratio of 10: 1 (V/W), add alkali, alkali concn control 3～10%, under the room temperature and lixiviate during high temperature, promptly under the room temperature, stirring and leaching 15～30 hours, during 70～90 ℃ of temperature, stirring and leaching 1～3 hour, centrifugal afterwards removal solid substance, collect filtrate, and be neutralized to subacidity with phosphoric acid buffer.
The preparation of zymin: described zymin mainly is zytase (EC3.2.1.8), it can be produced by solid-state or liquid state fermentation method respectively by microorganism next life, and the zytase of Chan Shenging can be prepared into partially purified zymin such as ammonium sulfate enzyme powder, ethanol sedimentation enzyme powder, acetone enzyme powder or the pure enzyme behind sephadexG-100 and DEAC-sephadex-A50 purifying etc. by fermentation.These zymins may be used to produce functional low polyxylose.
Enzyme digestion reaction: the filtrate after the neutralization is the solution based on hemicellulose.Zymin behind above-mentioned enzyme powder or the purifying adds enzyme by 1: 0.2～0.7 (g/Iu) hydrolyzed hemicellulose, the enzyme digestion reaction condition is: 35～55 ℃ of temperature, PH4～7, reaction times was controlled in 2 hours, with the product polymerization degree is foundation, this moment, the product polymerization degree was 2～10, was warming up to 100 ℃ after reaction finishes, and enzyme is lived lose.
The preparation of xylo-oligosaccharide: the hydrolyzed solution of described enzyme is again through decolouring, and ion-exchange, concentrate etc. gets final product to such an extent that xylo-oligosaccharide is main mixture, wherein contains a small amount of monose; Xylo-oligosaccharide ℃ following crystallization in low temperature＜8 after concentrating gets the xylo-oligosaccharide crystallization.The latter is the purified product of xylo-oligosaccharide.
The grouping experiment data:
| Experiment alkali temperature raising degree ℃ alkali is carried time h hydrolysis temperature ℃ enzymolysis time h reaction PH | |
| 1 70 1.5 40 2 90 2.0 55 3 80 1.0 35 | 2 4.0 6 7.0 4 5.5 |
The Analysis and Identification of product: the obtained functional low polyxylose of the present invention can detect as moving phase with the warm and fine water of second by a certain percentage through HPLC, and product is based on xylo-bioses as a result; Also available TLC method is a carrier with silica gel G-250, with propyl carbinol: pyridine: water=6: 4: 3 exhibition layers, again with aniline, pentanoic colour developing, but also analysed preparation component.These two kinds of methods are quick and convenient.
In addition, with collecting the solid of raw material after alkaline purification, can prepare CMC with a green acetic acid reaction.
Claims (4)
1. an enzyme process prepares the production technique of functional low polyxylose, and it may further comprise the steps:
A. corn cob, straw, bagasse, wheat straw powder or straw carried out drying, pulverize, sieving makes raw material;
B. above-mentioned raw materials soaks, dewaters with clear water, at room temperature uses the alkali stirring and leaching again 15～30 hours, and stirring and leaching is 1～3 hour in the time of 70～90 ℃, removes solid substance, collects filtrate, and is neutralized to subacidity with acid buffer, obtains hemicellulose solution;
C. add the zymin hydrolyzed hemicellulose behind enzyme powder or the purifying in above-mentioned hemicellulose solution, it is main mixture that the hydrolyzed solution of enzyme gets oligomeric xylo-bioses through decolouring, ion-exchange, process such as concentrate again.
D. above-mentioned zymin mainly is zytase (EC3.2.1.8), it can be made by solid-state or liquid state fermentation method respectively by microorganism, and the zytase of Chan Shenging can be prepared into partially purified zymin or the pure enzyme behind sephadex G-100 and DEAE-sephadex-A50 purifying by fermentation.
2. enzyme process according to claim 1 prepares the production technique of functional low polyxylose, it is characterized in that: corn cob or straw raw material at first should screen removes go mouldy composition and kept dry, fully soak with clear water, centrifugal removal moisture after the swelling with the solid-to-liquid ratio of 10: 1 (V/W), adds alkali again, alkali concn is controlled at 3～10%, lixiviate when reaching high temperature under the room temperature, afterwards, centrifugal removal solid substance, collect filtrate, and neutralize with phosphoric acid buffer.
3. enzyme process according to claim 1 prepares the production technique of functional low polyxylose, it is characterized in that: the zymin behind above-mentioned enzyme powder or the purifying adds enzyme by 1: 0.2～0.7 (g/Iu) hydrolyzed hemicellulose, the enzyme digestion reaction condition is: 35～55 ℃ of temperature, PH4～7, reaction times was controlled in 2 hours, was foundation with the product polymerization degree, and this moment, the product polymerization degree was 2～10, be warming up to 100 ℃ after reaction finishes, enzyme lived lose.
4. enzyme process according to claim 1 prepares the production technique of functional low polyxylose, it is characterized in that: the hydrolyzed solution of described enzyme gets final product to such an extent that xylo-oligosaccharide is main mixture through decolouring, ion-exchange, concentrate etc. again, wherein contain a small amount of monose, xylo-oligosaccharide ℃ following crystallization in low temperature＜8 after concentrating gets the xylo-oligosaccharide crystallization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN99126547A CN1116419C (en) | 1999-12-16 | 1999-12-16 | Enzymolysis process for preparing functional oligoxylose |
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| CN99126547A CN1116419C (en) | 1999-12-16 | 1999-12-16 | Enzymolysis process for preparing functional oligoxylose |
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| CN1266633A CN1266633A (en) | 2000-09-20 |
| CN1116419C true CN1116419C (en) | 2003-07-30 |
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Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100563905B1 (en) * | 2002-10-04 | 2006-03-23 | 송미란 | Food containing pith of corn stalk |
| CN101381753B (en) * | 2008-10-28 | 2012-05-30 | 上海师范大学 | Method for preparing rice husk xylo-oligosaccharides |
| CN101843329B (en) * | 2009-12-30 | 2013-01-02 | 吉林大学 | Method for preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme |
| CN101748157B (en) * | 2010-01-08 | 2012-07-25 | 安徽丰原发酵技术工程研究有限公司 | Corn cob acid hydrolysis residue and straw alkali cooking residue mixing enzymolysis method |
| CN101781669B (en) * | 2010-03-22 | 2013-07-17 | 苏州工业园区尚融科技有限公司 | Method for preparing high-purity xylo-oligosaccharide by adopting straws |
| CN102399842B (en) * | 2011-11-15 | 2014-10-29 | 上海师范大学 | Method for extracting xylo-oligosaccharide from straws |
| CN103467627A (en) * | 2013-09-14 | 2013-12-25 | 大连工业大学 | Preparation method for corn stalk hemicellulose |
| CN103614435B (en) * | 2013-12-02 | 2016-05-04 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of method of preparing xylo-oligosaccharide from bagasse |
| CN103757072A (en) * | 2014-01-10 | 2014-04-30 | 杭州师范大学 | Method for preparing xylan and xylo-oligosaccharide from rice straw |
| CN104357516A (en) * | 2014-11-07 | 2015-02-18 | 宜宾雅泰生物科技有限公司 | Process for producing food-grade xylo-oligosaccharide from viscose fiber squeezed alkali liquor |
| CN104928331A (en) * | 2015-07-08 | 2015-09-23 | 青岛嘉瑞生物技术有限公司 | Technology for preparing functional xylo-oligosaccharide by comprehensively utilizing wheat straw |
| CN108354061B (en) * | 2018-02-28 | 2021-07-16 | 苏州昆蓝生物科技有限公司 | Production method of intestinal regulator |
| CN109053824B (en) * | 2018-09-21 | 2020-11-24 | 安阳市豫鑫木糖醇科技有限公司 | Method for removing colloid in xylose solution by using enzyme preparation |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0315364A (en) * | 1989-06-12 | 1991-01-23 | Yamamoto Sangyo:Kk | Preparation of soluble dietary fiber made of extracted coffee ground |
| CN1144457A (en) * | 1994-03-02 | 1997-03-05 | 诺沃挪第克公司 | Processing plant material with xylanase |
| CN1233614A (en) * | 1999-04-12 | 1999-11-03 | 中国科学院化工冶金研究所 | Method for producing active oligomeric xylose |
-
1999
- 1999-12-16 CN CN99126547A patent/CN1116419C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0315364A (en) * | 1989-06-12 | 1991-01-23 | Yamamoto Sangyo:Kk | Preparation of soluble dietary fiber made of extracted coffee ground |
| CN1144457A (en) * | 1994-03-02 | 1997-03-05 | 诺沃挪第克公司 | Processing plant material with xylanase |
| CN1233614A (en) * | 1999-04-12 | 1999-11-03 | 中国科学院化工冶金研究所 | Method for producing active oligomeric xylose |
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| CN1266633A (en) | 2000-09-20 |
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