CN1266633A - Enzymolysis process for preparing functional oligoxylose - Google Patents
Enzymolysis process for preparing functional oligoxylose Download PDFInfo
- Publication number
- CN1266633A CN1266633A CN99126547A CN99126547A CN1266633A CN 1266633 A CN1266633 A CN 1266633A CN 99126547 A CN99126547 A CN 99126547A CN 99126547 A CN99126547 A CN 99126547A CN 1266633 A CN1266633 A CN 1266633A
- Authority
- CN
- China
- Prior art keywords
- enzyme
- production technology
- straw
- xylo
- oligosaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
An enzymolysis process for preparing oligoxylose used to make functional food and food additive using corn cob, maize stalk and rice straw as raw materials includes screening, drying, pulverizing, sieving,soaking in water, drying, mixing with alkali, lixiviating, filtering, neutralizing filtrate to weak acidity, adding enzyme powder or enzymolyzing agent to the obtained semi-cellulose solution, enzymolysis, decolouring, ion exchange, and concentrating to obtain mixture whose main component is oligodixylose or its crystal.
Description
The present invention relates to a kind of preparation method of functional oligose, specifically is the production technology that enzyme process prepares functional low polyxylose.
Compound sugar claims oligosaccharides again, is to be formed by 2-10 monose molecule dehydrating condensation, owing to the formation glycan molecule is different with combination all titles is arranged, and its physicochemical property and physiological function are also had nothing in common with each other.The called function compound sugar is meant the people, animals and plants have the compound sugar of special physiological effect, sugariness generally has only about the 30%-50% of sucrose, because of its indigestibility, lower calorific value, be difficult for causing blood sugar with it as sweetener, insulin raises, can not cause fat yet, some compound sugar is absorbed by human consumption because of difficulty, so can directly go into large intestine by small intestine, and play the effect of dietary fiber, and it can only be for the utilization of bifid lactobacillus can not be utilized by the enteron aisle putrefactivebacteria, thereby (this class compound sugar comprises isomaltose to have whole intestines effect, xylo-oligosaccharide, FOS or the like).Some decompose the effect that the compound sugar that obtains has plant growth regulation and inducing plant generation antibiotics by plant or fungal cell wall polysaccharide, and xylo-oligosaccharide, oligomeric beta glucan etc. are wherein arranged.The specific function of compound sugar has caused the attention of academia, along with the discovery of various enzymes and the progress of isolation technics, the development of leap has been arranged for the research of compound sugar in the world, range of application is constantly opened up, and is that the Application and Development of the new functionalized compound sugar of background has become the new focus that biological technical field is studied with the high-tech.
Yet the current still Oilgosaccharkdes effect of noting most of xylo-oligosaccharide.As everyone knows, the bifid lactobacillus is the normal bacterium of a kind of human body intestinal canal, and baby due Bifidobacterium promptly occurred on 3rd or four, and had comparative advantage.Along with age increase Bifidobacterium reduces gradually, and putrefactivebacterias such as perfringens Fusobacterium, Escherichia coli roll up.To old age, enteron aisle is full of putrefactivebacteria, and Bifidobacterium almost disappears.Putrefactivebacteria decomposes COF in enteron aisle, produce poisonous variation sources such as ammonia, amine, hydrogen sulfide, scatol, indoles, phenols and nitrosamine, human body absorbs these toxin for a long time, can promote aging, bring out cancer, cause diseases such as artery sclerosis, liver obstacle.Bifidobacterium then plays an important role to the defendance health: 1. suppress the breeding of harmful intestinal tract microorganism, decompose carbohydrate and produce organic acid, keep the gut flora balance, promote intestines peristalsis, help preventing constipation: 2. not corrupt protein, do not generate harmful substance, promote protein digestibility to absorb; 3. synthetic B family vitamin, and promote some inorganic salts is utilized; 4. produce some material and can strengthen the immunity that improves body; 5. decompose carcinogenic substances prevents and suppresses the generation of tumour, also can prevent the savings of body inner cholesterol.
The object of the present invention is to provide a kind of method for preparing lower polyxylose of making functional food and food additives.
For achieving the above object, the technical solution used in the present invention:
A kind of enzyme process prepares the production technology of functional low polyxylose, and it may further comprise the steps:
A. corncob, straw, bagasse, wheat straw powder, straw etc. carried out drying, pulverize, sieving makes raw material;
B. above-mentioned raw materials soaks, dewaters with clear water, uses under the alkali room temperature stirring and leaching again 15～30 hours, and stirring and leaching is 1～3 hour in the time of 70～90 ℃, removes solid content, collects filtrate, and is neutralized to subacidity with acid buffer, obtains hemicellulose solution;
C. add the enzyme preparation hydrolyzed hemicellulose behind enzyme powder or the purifying in above-mentioned hemicellulose solution, it is main mixture that the hydrolyzate of enzyme gets oligomeric xylobiose through decolouring, ion-exchange, process such as concentrate again.
The also available flow process as shown in Figure 1 of above-mentioned production technology is represented:
With corncob, corn stalk, straw etc. as raw material through screening, dry, pulverize, sieving makes raw material, above-mentioned raw materials water-soaked, dehydration, with alkali stirring and leaching under room temperature and high temperature, remove solid content, collect filtrate, be neutralized to subacidity, get hemicellulose solution with acid, in the solution that makes, add enzyme powder or enzyme preparation hydrolyzed hemicellulose, the hydrolyzate of enzyme again through decolouring, ion-exchange, after concentrating oligomeric two wood sugars be main mixture.
Bacterium in the healthy human body alimentary canal has kind more than 100, and its quantity reaches more than 1,000 hundred million.Wherein Bifidobacterium takes advantage in enteron aisle, and Bifidobacterium how much in the inspection defecation, just can how judge health.The flora structure is near the young people in the long lived elder enteron aisle, and this is confirmed by Chinese scholars.Therefore if can adjust the gut flora structure, Bifidobacterium is had the advantage, undoubtedly to people's health and anti-ageing be useful, edible Oilgosaccharkdes is a kind of strong supplementary means, xylo-oligosaccharide has proved to have good bifidobacterium factor functional.At present, the domestic patent that does not also retrieve the like product of xylo-oligosaccharide.Enzyme preparation among the present invention is by black mold production, and raw materials for production are edible agriculture organic waste materials, and to the human body safety non-toxic, the functional low polyxylose of being produced can be at food, feed, and health care and plant protection aspect all hold out broad prospects.
Embodiment
The concrete technical process that provides below in conjunction with Fig. 2 describes in detail.
Raw material processing: the raw material in order to the production functional low polyxylose mainly is from agriculture organic waste materials, as corncob, corn stalk, bagasse, wheat straw powder, wheat bran, straw, peanut shell, jowar stalk etc., these raw materials all are rich in hemicellulose, and nontoxic to human body, it is safe and reliable to make functional low polyxylose by them.Raw material such as corncob, straw at first should screen removes the composition that goes mouldy, and kept dry, and after the classified again pulverizing, it is standby to cross 360 mesh sieves.
The preparation of hemicellulose: above-mentioned raw materials is fully soaked with clear water, and centrifugal removal moisture after the swelling is again with the solid-to-liquid ratio of 10: 1 (V/W), add alkali, alkali concn control 3～10%, under the room temperature and lixiviate during high temperature, promptly under the room temperature, stirring and leaching 15～30 hours, during 70～90 ℃ of temperature, stirring and leaching 1～3 hour, centrifugal afterwards removal solid content, collect filtrate, and be neutralized to subacidity with phosphate buffer.
The preparation of enzyme preparation: described enzyme preparation mainly is zytase (EC3.2.1.8), it can be produced by solid-state or liquid state fermentation method respectively by microorganism next life, and the zytase of Chan Shenging can be prepared into partially purified enzyme preparation such as ammonium sulfate enzyme powder, precipitation with alcohol enzyme powder, interior ketolase powder or the pure enzyme behind sephadexG-100 and DEAC-sephadex-A50 purifying etc. by fermentation.These enzyme preparations may be used to produce functional low polyxylose.
Enzyme digestion reaction: the filtrate after the neutralization is the solution based on hemicellulose.Enzyme preparation behind above-mentioned enzyme powder or the purifying adds enzyme by 1: 0.2～0.7 (g/Iu) hydrolyzed hemicellulose, the enzyme digestion reaction condition is: 35～55 ℃ of temperature, PH4～7, reaction time was controlled in 2 hours, with the product degree of polymerization is foundation, this moment, the product degree of polymerization was 2～10, was warming up to 100 ℃ after reaction finishes, and enzyme is lived lose.
The preparation of xylo-oligosaccharide: the hydrolyzate of described enzyme is again through decolouring, and ion-exchange, concentrate etc. gets final product to such an extent that xylo-oligosaccharide is main mixture, wherein contains a small amount of monose; Xylo-oligosaccharide ℃ following crystallization in low temperature＜8 after concentrating gets the xylo-oligosaccharide crystallization.The latter is the purified product of xylo-oligosaccharide.
The grouping experiment data:
| Experiment alkali temperature raising degree ℃ alkali is carried time h hydrolysis temperature ℃ enzymolysis time h reaction PH | |
| ????1??????70????????1.5 ????2??????90????????2.0 ????3??????80????????1.0 | ??????????????40??????????2???????4.0 ??????????????55??????????6???????7.0 ??????????????35??????????4???????5.5 |
The Analysis and Identification of product: the obtained functional low polyxylose of the present invention can detect as flowing with the warm and fine water of second by a certain percentage mutually through HPLC, and product is based on xylobiose as a result; Also available TLC method is a carrier with silica G-250, with n-butanol: pyridine: water=6: 4: 3 exhibition layers, again with aniline, diphenylamines colour developing, but also analytic product component.These two kinds of methods are quick and convenient.
In addition, with collecting the solid of raw material after alkali treatment, can prepare CMC with a green acetic acid reaction.
Claims (5)
1. an enzyme process prepares the production technology of functional low polyxylose, and it may further comprise the steps:
A. corncob, straw, bagasse, wheat straw powder, straw etc. carried out drying, pulverize, sieving makes raw material;
B. above-mentioned raw materials soaks, dewaters with clear water, at room temperature uses the alkali stirring and leaching again 15～30 hours, and stirring and leaching is 1～3 hour in the time of 70～90 ℃, removes solid content, collects filtrate, and is neutralized to subacidity with acid buffer, obtains hemicellulose solution;
C. add the enzyme preparation hydrolyzed hemicellulose behind enzyme powder or the purifying in above-mentioned hemicellulose solution, it is main mixture that the hydrolyzate of enzyme gets oligomeric xylobiose through decolouring, ion-exchange, process such as concentrate again.
2. enzyme process according to claim 1 prepares the production technology of functional low polyxylose, it is characterized in that: raw materials such as corncob, straw at first should screen removes go mouldy composition and kept dry, fully soak with clear water, centrifugal removal moisture after the swelling with the solid-to-liquid ratio of 10: 1 (V/W), adds alkali again, alkali concn is controlled at 3～10%, lixiviate when reaching high temperature under the room temperature, afterwards, centrifugal removal solid content, collect filtrate, and neutralize with phosphate buffer.
3. enzyme process according to claim 1 prepares the production technology of functional low polyxylose, it is characterized in that: described enzyme preparation mainly is zytase (EC3.2.1.8), it can be made by solid-state or liquid state fermentation method respectively by microorganism, and the zytase of Chan Shenging can be prepared into partially purified enzyme preparation or the pure enzyme behind sephadex G-100 and DEAE-sephadex-A50 purifying by fermentation.
4. the production technology for preparing functional low polyxylose according to claim 1 or 3 described enzyme process, it is characterized in that: the enzyme preparation behind above-mentioned enzyme powder or the purifying adds enzyme by 1: 0.2～0.7 (g/Iu) hydrolyzed hemicellulose, the enzyme digestion reaction condition is: 35～55 ℃ of temperature, PH4～7, reaction time was controlled in 2 hours, was foundation with the product degree of polymerization, and this moment, the product degree of polymerization was 2～10, be warming up to 100 ℃ after reaction finishes, enzyme lived lose.
5. enzyme process according to claim 4 prepares the production technology of functional low polyxylose, it is characterized in that: the hydrolyzate of described enzyme gets final product to such an extent that xylo-oligosaccharide is main mixture through decolouring, ion-exchange, concentrate etc. again, wherein contain a small amount of monose, xylo-oligosaccharide ℃ following crystallization in low temperature＜8 after concentrating gets the xylo-oligosaccharide crystallization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99126547A CN1116419C (en) | 1999-12-16 | 1999-12-16 | Enzymolysis process for preparing functional oligoxylose |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN99126547A CN1116419C (en) | 1999-12-16 | 1999-12-16 | Enzymolysis process for preparing functional oligoxylose |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1266633A true CN1266633A (en) | 2000-09-20 |
| CN1116419C CN1116419C (en) | 2003-07-30 |
Family
ID=5284450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN99126547A Expired - Fee Related CN1116419C (en) | 1999-12-16 | 1999-12-16 | Enzymolysis process for preparing functional oligoxylose |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1116419C (en) |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100563905B1 (en) * | 2002-10-04 | 2006-03-23 | 송미란 | Food containing pith of corn stalk |
| CN101781669A (en) * | 2010-03-22 | 2010-07-21 | 苏州工业园区尚融科技有限公司 | Method for preparing high-purity xylo-oligosaccharide by adopting straws |
| CN102399842A (en) * | 2011-11-15 | 2012-04-04 | 上海师范大学 | Method for extracting xylo-oligosaccharide from straws |
| CN101381753B (en) * | 2008-10-28 | 2012-05-30 | 上海师范大学 | Method for preparing rice husk xylo-oligosaccharides |
| CN101748157B (en) * | 2010-01-08 | 2012-07-25 | 安徽丰原发酵技术工程研究有限公司 | Corn cob acid hydrolysis residue and straw alkali cooking residue mixing enzymolysis method |
| CN101843329B (en) * | 2009-12-30 | 2013-01-02 | 吉林大学 | Method for preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme |
| CN103467627A (en) * | 2013-09-14 | 2013-12-25 | 大连工业大学 | Preparation method for corn stalk hemicellulose |
| CN103614435A (en) * | 2013-12-02 | 2014-03-05 | 广西壮族自治区农业科学院农产品加工研究所 | Method for preparing xylo-oligosaccharide from bagasse |
| CN103757072A (en) * | 2014-01-10 | 2014-04-30 | 杭州师范大学 | Method for preparing xylan and xylo-oligosaccharide from rice straw |
| CN104357516A (en) * | 2014-11-07 | 2015-02-18 | 宜宾雅泰生物科技有限公司 | Process for producing food-grade xylo-oligosaccharide from viscose fiber squeezed alkali liquor |
| CN104928331A (en) * | 2015-07-08 | 2015-09-23 | 青岛嘉瑞生物技术有限公司 | Technology for preparing functional xylo-oligosaccharide by comprehensively utilizing wheat straw |
| CN109053824A (en) * | 2018-09-21 | 2018-12-21 | 安阳市豫鑫木糖醇科技有限公司 | A method of with colloid in enzyme preparation removal xylose solution |
| CN108354061B (en) * | 2018-02-28 | 2021-07-16 | 苏州昆蓝生物科技有限公司 | Production method of intestinal regulator |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0315364A (en) * | 1989-06-12 | 1991-01-23 | Yamamoto Sangyo:Kk | Preparation of soluble dietary fiber made of extracted coffee ground |
| EP0746206B1 (en) * | 1994-03-02 | 2004-09-08 | Novozymes A/S | Processing plant material with xylanase |
| CN1087299C (en) * | 1999-04-12 | 2002-07-10 | 中国科学院化工冶金研究所 | Method for producing active oligomeric xylose |
-
1999
- 1999-12-16 CN CN99126547A patent/CN1116419C/en not_active Expired - Fee Related
Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100563905B1 (en) * | 2002-10-04 | 2006-03-23 | 송미란 | Food containing pith of corn stalk |
| CN101381753B (en) * | 2008-10-28 | 2012-05-30 | 上海师范大学 | Method for preparing rice husk xylo-oligosaccharides |
| CN101843329B (en) * | 2009-12-30 | 2013-01-02 | 吉林大学 | Method for preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme |
| CN101748157B (en) * | 2010-01-08 | 2012-07-25 | 安徽丰原发酵技术工程研究有限公司 | Corn cob acid hydrolysis residue and straw alkali cooking residue mixing enzymolysis method |
| CN101781669A (en) * | 2010-03-22 | 2010-07-21 | 苏州工业园区尚融科技有限公司 | Method for preparing high-purity xylo-oligosaccharide by adopting straws |
| CN101781669B (en) * | 2010-03-22 | 2013-07-17 | 苏州工业园区尚融科技有限公司 | Method for preparing high-purity xylo-oligosaccharide by adopting straws |
| CN102399842B (en) * | 2011-11-15 | 2014-10-29 | 上海师范大学 | Method for extracting xylo-oligosaccharide from straws |
| CN102399842A (en) * | 2011-11-15 | 2012-04-04 | 上海师范大学 | Method for extracting xylo-oligosaccharide from straws |
| CN103467627A (en) * | 2013-09-14 | 2013-12-25 | 大连工业大学 | Preparation method for corn stalk hemicellulose |
| CN103614435A (en) * | 2013-12-02 | 2014-03-05 | 广西壮族自治区农业科学院农产品加工研究所 | Method for preparing xylo-oligosaccharide from bagasse |
| CN103614435B (en) * | 2013-12-02 | 2016-05-04 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of method of preparing xylo-oligosaccharide from bagasse |
| CN103757072A (en) * | 2014-01-10 | 2014-04-30 | 杭州师范大学 | Method for preparing xylan and xylo-oligosaccharide from rice straw |
| CN104357516A (en) * | 2014-11-07 | 2015-02-18 | 宜宾雅泰生物科技有限公司 | Process for producing food-grade xylo-oligosaccharide from viscose fiber squeezed alkali liquor |
| CN104928331A (en) * | 2015-07-08 | 2015-09-23 | 青岛嘉瑞生物技术有限公司 | Technology for preparing functional xylo-oligosaccharide by comprehensively utilizing wheat straw |
| CN108354061B (en) * | 2018-02-28 | 2021-07-16 | 苏州昆蓝生物科技有限公司 | Production method of intestinal regulator |
| CN109053824A (en) * | 2018-09-21 | 2018-12-21 | 安阳市豫鑫木糖醇科技有限公司 | A method of with colloid in enzyme preparation removal xylose solution |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1116419C (en) | 2003-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1116419C (en) | Enzymolysis process for preparing functional oligoxylose | |
| CN105472994A (en) | Method of preparing feed additive | |
| RU2460312C2 (en) | Ballast substance | |
| CA2133748C (en) | Enzyme products for use in the improvement of feed value and conservation of fibrous crops | |
| CN111328923A (en) | Biological composite straw pellet feed and preparation method thereof | |
| CN112535245A (en) | Fish feed and production method thereof | |
| CN105725127B (en) | Water chestnut dietary fiber powder with prebiotics function and preparation method thereof | |
| CN105166394A (en) | Production method of forage-use ramie fodder burdening | |
| AU755179B2 (en) | Enzymatic modification of psyllium | |
| CN113057258B (en) | Environment-friendly organic pig feed and preparation method thereof | |
| CN108669330A (en) | A kind of fermentation crude fibre formula for pig forage and preparation method thereof | |
| KR19990009598A (en) | Method for preparing indigestible high-lining texturine with high enzyme resistance | |
| CN114532444A (en) | Fibrous thallus protein feed and preparation method thereof | |
| JPH08332032A (en) | Peroral enzyme preparation for animal and its use | |
| CN1052860C (en) | High protein saccharified feeds and preparation thereof | |
| CN103865900B (en) | A kind of feed complex enzyme containing alkalescent xylanase and preparation method thereof | |
| CN106804942A (en) | A kind of piglet fermented feed and preparation method thereof | |
| CN109259086A (en) | The nutrient rice and preparation method thereof of adjustable constipation | |
| CN110892946A (en) | Low-feed-meat-ratio livestock green feed for plain areas and production process thereof | |
| CN109567202A (en) | A kind of extracting method of barley green | |
| CN110041385B (en) | Preparation process for extracting stevioside from stevia rebaudiana through steam explosion treatment and fermented feed | |
| CN106804908A (en) | One kind preventing and treating nephritic dropsy banana stem leaves fermentation cattle feed | |
| CN113826769A (en) | Growth-promoting pig feed | |
| CN105918691A (en) | Feed additive for promoting digestion of chicken | |
| CN113712128A (en) | Pig feed for improving immunity of organism and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20030730 Termination date: 20161216 |