CN101843329B - Method for preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme - Google Patents
Method for preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme Download PDFInfo
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- CN101843329B CN101843329B CN2009102181551A CN200910218155A CN101843329B CN 101843329 B CN101843329 B CN 101843329B CN 2009102181551 A CN2009102181551 A CN 2009102181551A CN 200910218155 A CN200910218155 A CN 200910218155A CN 101843329 B CN101843329 B CN 101843329B
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Abstract
The invention provides a preparation method of dietary fiber by adopting corn bran hydrolyzed by a multifunctional enzyme. The preparation method comprises the following steps of: adding the corn bran into NaOH and soaking darkly, sterilizing at high pressure, adding the multifunctional enzyme with peptidase and esterase activities for hydrolyzing ester bonds and peptide bonds on the cell wall, centrifugally taking precipitate, decoloring and rinsing, drying at the temperature below 60 DEG C, crushing and screening by adopting a 80-100 mesh sieve to obtain the dietary fiber. The dietary fiber which has the expansion force up to 10-15ml/g and the water-holding capacity of 9-11g/g and is superior to other methods is prepared by utilizing the corn bran as a by-product in the process of grain processing or fuel ethanol production and the hydrolysis of the multifunctional enzyme with peptidase and esterase activities. The invention utilizes the enzyme, has simple process, saves time and energy and increases the additional value of the corn by-products.
Description
Technical field
The present invention relates to a kind of production method of food fibre, a kind of method of preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme especially is provided, utilize the multifunctional enzyme of engineering bacterium expression to obtain food fibre, belong to the corn deep processing technology field.
Background technology
Mainly contain vegetable polysaccharides in the cereal grass cell walls; Protein, xylogen and a small amount of phenolic compound etc.In recent years, the investigator can adopt amylase and proteolytic enzyme to remove starch and the protein that remains in the corn bran, come the leaching semi Mierocrystalline cellulose with sodium hydroxide solution again, then utilize the different solubilities of acid base pair Mierocrystalline cellulose, hemicellulose, xylogen etc. to come it is separated, can obtain different corn dietary fiber products, the performance of product has very big difference.Have literature research to show, domestic utilization to maize peel only limits to extract food fibre, and mainly takes chemical treatment method, such as strong acid, highly basic, alcohol etc.The characteristics of chemical method are that cost is lower, but the acid-alkali treatment process runs off a large amount of water-soluble dietary fibres, and retention ability and the bulging force of product are lower
Summary of the invention
The present invention discloses a kind of method of preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme, utilizes the byproduct maize peel of grain processing or Maize Production, produces food fibre by multi-functional enzyme process, and its bulging force reaches 10-15ml/g, and retention ability is 9-11g/g.
The invention provides the method for extracting food fibre, may further comprise the steps:
1) with ground corn skin 60～80 orders, add 0.6%NaOH, solid-to-liquid ratio is 1: (5-7) (W/V), lucifuge was soaked 1～3 hour;
2) with 121 ℃ of high pressure 0.105PKa sterilizations of soak 30-60min;
3) after soak solution is cooled to 50 ℃, regulate pH to 8, add multifunctional enzyme 20ul by every ml soak solution, ester bond and peptide bond on the hydrolysis cell walls are put under 50 ℃ of conditions of shaking table and were reacted 2～3 hours; The centrifugal 10min of 5500r;
4) get precipitation, 60-90 ℃ of solid-to-liquid ratio 1: 5-8 (W/V) heating, the hydrogen peroxide of adding final concentration 0.3% decoloured 30-60 minute, was washed to non-oxidative,
5) the 80-100 mesh sieve was pulverized in oven dry below 60 ℃ or naturally dried, obtained food fibre.
The multifunctional enzyme that uses among the preparation method of the present invention (APE1547) is a kind of engineering enzyme with peptase and esterase activity, prepare in the food fibre process at the hydrolysis maize peel, adopt a kind of enzyme, single reaction conditions, have and save time, laborsaving, the characteristics of save energy.
The food fibre of at present prior art acquisition, its bulging force is 2.5-3, retention ability 4.2-4.4; And the food fibre of the inventive method, its bulging force reaches 10-15ml/g, and retention ability is 9-11g/g.
Positively effect of the present invention is: the byproduct that utilizes grain processing or fuel ethanol production--maize peel, produce food fibre by the multifunctional enzyme hydrolysis that has simultaneously peptase and esterase activity, its bulging force reaches 10-15ml/g, retention ability is 9-11g/g, is better than the food fibre that other method is produced.And utilize a kind of enzyme, its technique is simple, and time-saving energy-saving has increased the added value of corn's by-products.
Embodiment
By following examples the present invention is described for example further.And do not limit the present invention in any way.Do not deviate from that those skilled in the art easily realize any change or change all will fall within the claim scope of the present invention.
Embodiment 1
1) 0.6%Na0H of adding 70L in the 80 order maize peels of 1kg soaked 1.5 hours;
2) with 121 ℃ of high pressure 0.105PKa sterilizations of soak 50min;
3) after soak solution is cooled to 50 ℃, regulate pH to 8, add multifunctional enzyme (APE1547) 20ul by every ml soak solution, ester bond and peptide bond on the hydrolysis cell walls are put under 50 ℃ of conditions of shaking table and were reacted 2 hours; The centrifugal 10min of 5500r;
4) get precipitation, 70 ℃ of solid-to-liquid ratio (W/V) heating in 1: 6, the hydrogen peroxide of adding final concentration 0.3% decoloured 45 minutes, was washed to non-oxidative, PH=9.0
5) the 80-100 mesh sieve was pulverized in oven dry below 60 ℃ or naturally dried, obtained food fibre.
Its bulging force reaches 14.5ml/g, and retention ability is 11g/g.
Embodiment 2
1) 0.6%Na0H of adding 650L in the 80 order maize peels of 100kg, lucifuge was soaked 1.5 hours;
2) with 121 ℃ of high pressure 0.105PKa sterilizations of soak 40min;
3) after soak solution is cooled to 50 ℃, regulate pH to 8, add multifunctional enzyme (APE1547) 20ul by every ml soak solution, ester bond and peptide bond on the hydrolysis cell walls are put under 50 ℃ of conditions of shaking table and were reacted 2 hours; The centrifugal 10min of 5500r;
4) get precipitation, solid-to-liquid ratio 1: 6 (W/V), PH=8.5 heats 90 ℃, adds the hydrogen peroxide of final concentration 0.3%, decolours 30 minutes, is washed to non-oxidative,
5) the 80-100 mesh sieve was pulverized in oven dry below 60 ℃ or naturally dried, obtained food fibre.
Its bulging force reaches 12ml/g, and retention ability is more than the 10g/g.
Embodiment 3
1) 0.6%NaOH of adding 70L in the 80 order maize peels of 10kg, lucifuge was soaked 1.5 hours;
2) with 121 ℃ of high pressure 0.105PKa sterilizations of soak 30min;
3) after soak solution is cooled to 50 ℃, regulate pH to 8, add multifunctional enzyme (APE1547) 20ul by every ml soak solution, ester bond and peptide bond on the hydrolysis cell walls are put under 60 ℃ of conditions of shaking table and were reacted 2 hours; The centrifugal 10min of 5500r;
4) get precipitation, 70 ℃ of solid-to-liquid ratio (W/V) heating in 1: 6, the hydrogen peroxide of adding final concentration 0.3% decoloured 50 minutes, was washed to non-oxidative, PH=8.5
5) the 80-100 mesh sieve was pulverized in oven dry below 60 ℃ or naturally dried, obtained food fibre.
Its bulging force reaches 10ml/g, and retention ability is 9g/g.
Claims (1)
1. the preparation method of multifunctional enzyme hydrolysis corn dietary fiber may further comprise the steps:
1) add 0.6%NaOH in 80 order maize peels, solid-to-liquid ratio is 1: (5-7) (W/V), and lucifuge was soaked 1～3 hour;
2) with 121 ℃ of high pressure 105KPa sterilizations of soak 30-60min;
3) after soak solution is cooled to 50 ℃, regulate pH to 8, add the APE1547 multifunctional enzyme 20 μ L that have simultaneously proteolytic enzyme and esterase activity by every mL soak solution, ester bond and peptide bond on the hydrolysis cell walls are put under 50 ℃ of conditions of shaking table and were reacted 2～3 hours; 5500r/min, centrifugal 10min;
4) taking precipitate decolouring washing, solid-to-liquid ratio is 1: 5 (W/V), PH=9.0 heats 70 ℃, add the hydrogen peroxide of final concentration 0.3%, decoloured 45 minutes, be washed to non-oxidative, the 80-100 mesh sieve was pulverized in oven dry below 60 ℃ or naturally dried, obtained food fibre.
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| CN2009102181551A CN101843329B (en) | 2009-12-30 | 2009-12-30 | Method for preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme |
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| CN2009102181551A CN101843329B (en) | 2009-12-30 | 2009-12-30 | Method for preparing dietary fiber by adopting corn bran hydrolyzed by multifunctional enzyme |
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| CN101843329A CN101843329A (en) | 2010-09-29 |
| CN101843329B true CN101843329B (en) | 2013-01-02 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105995489A (en) * | 2016-05-30 | 2016-10-12 | 王文杰 | Edible mushroom fermented corn flour and corn product |
| CN108977895B (en) * | 2018-09-29 | 2020-09-29 | 齐齐哈尔大学 | Method for preparing corn bract fiber by using biological enzyme |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266633A (en) * | 1999-12-16 | 2000-09-20 | 合肥联合大学 | Enzymolysis process for preparing functional oligoxylose |
| CN1537946A (en) * | 2003-10-24 | 2004-10-20 | 邱芳萍 | Method of extracting dietary fiber from sogbean drges and chewing tablet |
| CN101411501A (en) * | 2008-12-05 | 2009-04-22 | 吉林大学 | Preparation technique for producing dietary fiber of high activity using acidified maize peel |
| CN101455357A (en) * | 2008-12-31 | 2009-06-17 | 深圳职业技术学院 | Soya-dregs water-soluble diet fiber preparation method using ultrafiltration and spray drying |
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2009
- 2009-12-30 CN CN2009102181551A patent/CN101843329B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1266633A (en) * | 1999-12-16 | 2000-09-20 | 合肥联合大学 | Enzymolysis process for preparing functional oligoxylose |
| CN1537946A (en) * | 2003-10-24 | 2004-10-20 | 邱芳萍 | Method of extracting dietary fiber from sogbean drges and chewing tablet |
| CN101411501A (en) * | 2008-12-05 | 2009-04-22 | 吉林大学 | Preparation technique for producing dietary fiber of high activity using acidified maize peel |
| CN101455357A (en) * | 2008-12-31 | 2009-06-17 | 深圳职业技术学院 | Soya-dregs water-soluble diet fiber preparation method using ultrafiltration and spray drying |
Non-Patent Citations (1)
| Title |
|---|
| 金毓峑等.酶法制取水溶性膳食纤维的实验研究.《北京工业大学学报》.2004,(第01期), * |
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