CN111617151A - Traditional Chinese medicine for preventing and treating senile dementia and production method thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine for preventing and treating senile dementia and a production method thereof, belonging to the field of traditional Chinese medicines. The preparation method comprises extracting the above materials with water, precipitating with ethanol, concentrating, and making into oral liquid, granule, capsule, and tablet. The composition has effects in invigorating kidney, replenishing essence, promoting blood circulation, dispelling blood stasis, tranquilizing mind, regulating activity of acetylcholinesterase and acetylcholinesterase transferase in cholinergic nervous system of brain, scavenging oxygen free radicals, and removing aluminum. Can be used for preventing and treating amnesia, insomnia, hypomnesis, activity change of cerebral acetylcholinesterase (AchE) and acetylcholine transferase (ChAT), scavenging oxygen free radicals, and removing aluminum due to senile dementia.

Description

Traditional Chinese medicine for preventing and treating senile dementia and production method thereof

Technical Field

The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine for preventing and treating senile dementia and a production method thereof.

Background

With the development of material civilization, the living standard of people is higher and higher, the service life is longer and longer, and aging becomes a life reality. The accompanying senile diseases, especially senile dementia, become not only personal unfortunate but also burden on families and encumbrance of society. In developed countries, senile dementia has become the fourth cause of death, and in China, due to the accelerated aging process, the prevalence rate of senile dementia rapidly increases, and particularly, the prevalence rate of senile dementia above 65 years is close to the level of developed countries. At present, millions of patients with senile dementia in China are treated. This catastrophic disease is a major problem that has to be addressed by all countries in the world. According to the 91 st meeting of the Xiangshan meeting of 98 years and the meeting report of 'century-spanning brain science-senile dementia pathogenesis and prevention' gathering 7 medical, biological and physical communities and overseas students, no ideal method for treating senile dementia is provided all over the world, and no specific medicine is provided.

Senile dementia (AD), also known as alzheimer's disease, has symptoms of disorders such as language, memory, visual space, emotion or personality, sleep, cognition (including abstract thinking, computation, judgment, executive ability), etc. The causes and pathogenesis of senile dementia (AD) are unknown, and there are many theories proposed by scholars in medical circles at home and abroad, such as the theory of cholinergic neurological disorder, the theory of gene, the theory of free radical, the theory of aluminum poisoning, and the like, and no radical medicine exists yet. Although some traditional Chinese medicines are prepared to treat senile dementia mainly according to the theory of traditional Chinese medicine and the theory of cholinergic neurological disorder in recent years, the traditional Chinese medicines have satisfactory effects, such as: 1. chinese patent: application (patent) No.: 200510002971 application (patentee): jilin, Liaoyuan Yadong pharmaceutical products company, address: friendship road 33 in Liaoyuan city, Jilin, abstract: a traditional Chinese medicine preparation for treating senile dementia comprises the following traditional Chinese medicine raw materials in parts by weight: 20-40 parts of prepared fleece-flower root, 20-30 parts of lotus leaf, 10-25 parts of cistanche, 10-25 parts of earthworm and 5-20 parts of uniflower swisscentaury root. The preparation method comprises processing radix Polygoni Multiflori Preparata into fluid extract; decocting folium Nelumbinis, Cistanchis herba, and radix Rhapontici to obtain fluid extract; mixing the above two extracts, making into dry extract, treating Lumbricus to obtain dry extract, mixing with the above dry extract, and making into final product. 2. Chinese patent: application (patent) No.: 200910064495 application (patentee): waiting for vibration, address: three groups of high temple countryside fine villages in south-south province, south-yang city, abstract: a Chinese medicine in the form of pill for treating senile dementia, ischia, lumbar vertebra, furuncle, rheumatism, etc is prepared from fresh whole toad and bone and meat of weasel through proportional mixing. 3. Chinese patent: application (patent) No.: 200810056128.4 application (patentee): beijing china kogyo and pharmaceutical technology ltd, address: unit 508 of stabilizing Yongzhong 28 # Mengdong street and east building 1 of mansion in Tokyo area of Beijing city, abstract: a pharmaceutical composition for treating senile dementia is mainly prepared from ten traditional Chinese medicines including acanthopanax, epimedium, white peony root, angelica, rhizoma ligustici wallichii, bighead atractylodes rhizome, rhizoma alismatis, roasted polygala tenuifolia, poria cocos, rhizoma acori graminei and the like. 4. Chinese patent: application (patent) No.: 98112741.X application (patent rights): great gentleys, address: xilu labour of Changsha, Hunan province 107, abstract: an oral liquid for preventing and treating senile dementia is prepared from rhizoma Acori Graminei, radix astragali, cortex et radix Polygalae, radix Saposhnikoviae, radix Gentianae Marcrophyllae, Poria, fructus Alpinae Oxyphyllae, flos Chrysanthemi, herba Schizonepetae, Notopterygii rhizoma, and fructus Gardeniae by decocting, distilling and refining. However, different traditional Chinese medicine formulas can prepare preparations with different effects. Modern medical research shows that the pathological changes of senile dementia are encephalatrophy, cortical nerve cell reduction, a large amount of neurofibrillary tangles, degeneration and senile plaque pathological changes in cerebral cortex and hippocampus, which lead to the functional disorder of cholinergic nervous system related to memory and cognition, the decrease of neurotransmitter-acetylcholine level and the change of activity of related enzyme "acetylcholinesterase" (TchE), so that the disease is also called encephalatrophy in traditional Chinese medicine, and meanwhile, the pathological changes of senile dementia are found, and the pathological changes of senile plaque are about 30 times of normal patients with large amount of accumulated aluminum in brains of the patients, and what role of aluminum in senile plaque is accumulated in senile plaque? The other main component of the senile plaque is insoluble AB amyloid protein which is converted from soluble precursor protein and is deposited in the senile plaque, and the research finds that the amino acid fragment at the 25-35 position of the AB protein is an insoluble toxic fragment, and aluminum can inhibit the AB25-35 from being cracked into soluble AB29-35, so that the deposition of AB25-35 is further promoted, the insoluble protein in the senile plaque is increased continuously, the dysfunction of the cholinergic nervous system related to memory and cognition is caused, and the onset and aggravation of AD are caused; once aluminum has entered the brain, it is difficult to remove, as to what role aluminum plays in age spots? What is its direct basis in the onset of AD? According to the theory of traditional Chinese medicine and the experimental results of the invention, the medicine has the effects of clearing away heat and toxic materials, tonifying spleen and promoting diuresis, protecting liver and kidney, promoting blood circulation and removing blood stasis, promoting the excretion of excessively high-accumulated aluminum in the brain of an AD patient, eliminating excessive harmful superoxide anion free radicals (O2) and the like, so that the activity of acetylcholinesterase and the content of acetylcholine neurotransmitter in the brain are regulated, and the memory and cognitive ability of the patient are restored and enhanced, thereby achieving the purpose of treatment. According to the syndrome differentiation of the traditional Chinese medicine, the senile dementia is a systemic disease and is caused by deficiency of essence, qi and blood, hyperemia of the marrow sea, loss of nourishment of the brain, or internal obstruction of qi, fire and phlegm stasis which disturb the clear orifices. The disease belongs to the categories of 'dull disease', 'amnesia' and the like in the traditional Chinese medicine, the traditional Chinese medicine considers that the disease is located in the brain, is closely related to the dysfunction of the heart, the liver, the spleen and the kidney, and considers that the pathogenesis is marrow reduction and brain elimination, the loss of mental mechanism, the empty deficiency of brain marrow and deficiency of qi and blood, the heart and the mind are malnutrition caused by deficiency of the essence and excess of the origin, the heart, the liver, the spleen and the kidney deficiency are taken as the origin, phlegm stasis is endogenous, and the reverse disorder of qi and blood is taken as the secondary sign, the kidney is mainly used for tonifying kidney and replenishing essence, and the kidney is used as the sea of the marrow, so the kidney tonifying becomes the important principle for; eliminating pathogens mainly by promoting blood circulation and removing blood stasis; the concurrent application of tonics and purgatives is mainly to tonify the kidney, activate blood and remove blood stasis, so that the tonics, replenishing essence, activating blood and removing blood stasis are the theoretical basis of the traditional Chinese medicine for treating the senile dementia.

Disclosure of Invention

The invention aims to overcome the defects of the prior art in preventing and treating the senile dementia, and provides a traditional Chinese medicine preparation for preventing and treating the senile dementia, which has the advantages of good curative effect, low cost and no side effect, and a production method and application thereof.

A traditional Chinese medicine for preventing and treating senile dementia is prepared from the following traditional Chinese medicine raw materials in parts by weight: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry. The fleece-flower root is firstly processed by a high pressure method to obtain the prepared fleece-flower root, the process is that the dried fleece-flower root slices are soaked with water to be wet, then put into a steam pressure cooker at the temperature of 105 ℃ and 121 ℃ for 2-10 hours, and then taken out to be dried to obtain the prepared fleece-flower root.

The Chinese medicinal composition can be made into oral liquid, granule, capsule and tablet.

A production method of a traditional Chinese medicine oral liquid for preventing and treating senile dementia comprises the following steps:

step 1: weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry;

step 2: putting the raw materials in the step 1 into a traditional Chinese medicine extraction tank, adding 10-15 times of weight of water, heating and boiling for 1-2 hours, discharging liquid medicine, then adding 5-10 times of weight of water into the medicine tank, heating and boiling again for 0.5-1 hour, discharging the liquid medicine, converging and filtering to obtain liquid medicine;

and step 3: putting the liquid medicine into a concentration tank, heating and concentrating to 0.2-0.3 time of volume, and cooling the concentrated liquid medicine;

and 4, step 4: adding 90-95% ethanol with volume of 3-6 times of the cooled concentrated liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a filtrate;

and 5: putting the filtrate into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain an initial liquid medicine, cooling the initial liquid medicine, and filtering to obtain a filtered liquid medicine;

step 6: dripping 3-5% edible gelatin solution into the filtered liquid medicine while stirring, stopping stirring until white precipitate appears, and filtering to remove precipitate to obtain tannin-removed liquid medicine;

and 7: adding 95% ethanol with volume of 3-6 times of the tannin-removed liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a secondary filtrate;

and 8: placing the secondary filtered liquid medicine into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain secondary liquid medicine, cooling the secondary liquid medicine, and filtering to obtain secondary filtered liquid medicine;

and step 9: adding sucrose into the twice-filtered medicinal liquid, adding water to 1000ml, and obtaining oral medicinal liquid with sugar concentration of 20%;

step 10: filling the oral liquid medicine, autoclaving the filled liquid medicine, then inspecting, and packaging to obtain the finished product.

In the step 1, the polygonum multiflorum is processed by a high pressure method to obtain the prepared polygonum multiflorum, the process is that the dried polygonum multiflorum slices are soaked in water and are wet, the dried polygonum multiflorum slices are put into a steam pressure cooker at the temperature of 105 ℃ and 121 ℃ for 2-10 hours, and then the polygonum multiflorum slices are taken out and dried to obtain the prepared polygonum multiflorum.

In the step 10, during filling, 10 ml/bottle and 100 ml/bottle are sterilized under high pressure at 105 ℃ for 30-40 minutes.

A production method of traditional Chinese medicine granule for preventing and treating senile dementia comprises the following steps,

step 1: weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry;

step 2: putting the raw materials in the step 1 into a traditional Chinese medicine extraction tank, adding 10-15 times of weight of water, heating and boiling for 1-2 hours, discharging liquid medicine, then adding 5-10 times of weight of water into the medicine tank, heating and boiling again for 0.5-1 hour, discharging the liquid medicine, converging and filtering to obtain liquid medicine;

and step 3: putting the liquid medicine into a concentration tank, heating and concentrating to 0.2-0.3 time of volume, and cooling the concentrated liquid medicine;

and 4, step 4: adding 90-95% ethanol with volume of 3-6 times of the cooled concentrated liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a filtrate;

and 5: putting the filtrate into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain an initial liquid medicine, cooling the initial liquid medicine, and filtering to obtain a filtered liquid medicine;

step 6: dripping 3-5% edible gelatin solution into the filtered liquid medicine while stirring, stopping stirring until white precipitate appears, and filtering to remove precipitate to obtain tannin-removed liquid medicine;

and 7: adding 95% ethanol with volume of 3-6 times of the tannin-removed liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a secondary filtrate;

and 8: placing the secondary filtered liquid medicine into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain secondary liquid medicine, cooling the secondary liquid medicine, and filtering to obtain secondary filtered liquid medicine;

and step 9: heating and concentrating the secondary filtered liquid medicine in a concentrating pan to obtain pasty medicine;

step 10: and (3) adding 940g of cane sugar into the pasty medicine, then putting the pasty medicine into a granulator for granulation to obtain medicine granules, and quantitatively packaging the medicine granules to obtain a finished product.

A production method of a traditional Chinese medicine capsule for preventing and treating senile dementia comprises the following steps:

step 1: weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry;

step 2: putting the raw materials in the step 1 into a traditional Chinese medicine extraction tank, adding 10-15 times of weight of water, heating and boiling for 1-2 hours, discharging liquid medicine, then adding 5-10 times of weight of water into the medicine tank, heating and boiling again for 0.5-1 hour, discharging the liquid medicine, converging and filtering to obtain liquid medicine;

and step 3: putting the liquid medicine into a concentration tank, heating and concentrating to 0.2-0.3 time of volume, and cooling the concentrated liquid medicine;

and 4, step 4: adding 90-95% ethanol with volume of 3-6 times of the cooled concentrated liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a filtrate;

and 5: putting the filtrate into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain an initial liquid medicine, cooling the initial liquid medicine, and filtering to obtain a filtered liquid medicine;

step 6: dripping 3-5% edible gelatin solution into the filtered liquid medicine while stirring, stopping stirring until white precipitate appears, and filtering to remove precipitate to obtain tannin-removed liquid medicine;

and 7: adding 95% ethanol with volume of 3-6 times of the tannin-removed liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a secondary filtrate;

and 8: placing the secondary filtered liquid medicine into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain secondary liquid medicine, cooling the secondary liquid medicine, and filtering to obtain secondary filtered liquid medicine;

and step 9: heating and concentrating the secondary filtered liquid medicine in a concentrating pan to obtain pasty medicine;

step 10: adding adjuvants into the paste, pulverizing, drying, mixing, making into capsule, and sealing with a can sealing machine to obtain the final product.

A production method of a traditional Chinese medicine tablet for preventing and treating senile dementia comprises the following steps:

step 1: weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry;

step 2: putting the raw materials in the step 1 into a traditional Chinese medicine extraction tank, adding 10-15 times of weight of water, heating and boiling for 1-2 hours, discharging liquid medicine, then adding 5-10 times of weight of water into the medicine tank, heating and boiling again for 0.5-1 hour, discharging the liquid medicine, converging and filtering to obtain liquid medicine;

and step 3: putting the liquid medicine into a concentration tank, heating and concentrating to 0.2-0.3 time of volume, and cooling the concentrated liquid medicine;

and 4, step 4: adding 90-95% ethanol with volume of 3-6 times of the cooled concentrated liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a filtrate;

and 5: putting the filtrate into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain an initial liquid medicine, cooling the initial liquid medicine, and filtering to obtain a filtered liquid medicine;

step 6: dripping 3-5% edible gelatin solution into the filtered liquid medicine while stirring, stopping stirring until white precipitate appears, and filtering to remove precipitate to obtain tannin-removed liquid medicine;

and 7: adding 95% ethanol with volume of 3-6 times of the tannin-removed liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a secondary filtrate;

and 8: placing the secondary filtered liquid medicine into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain secondary liquid medicine, cooling the secondary liquid medicine, and filtering to obtain secondary filtered liquid medicine;

and step 9: heating and concentrating the secondary filtered liquid medicine in a concentrating pan to obtain pasty medicine;

step 10: adding adjuvants into the paste medicine, pulverizing, drying, mixing, tabletting, and canning to obtain the final product.

Square solution:

1. saviae Miltiorrhizae radix is dried root and rhizome of Salvia miltiorrhiza Bunge of Labiatae, and has effects of promoting blood circulation, removing blood stasis, dredging channels, stopping bleeding, clearing heart fire, relieving restlessness, cooling blood, and resolving carbuncle. Modern pharmacological research shows that the salvia miltiorrhiza can improve microcirculation, has a regulating effect on central nerves, can relieve dysphoria and hyperactivity, and is beneficial to regulating neurasthenia, insomnia and dreaminess symptoms of patients.

2. Arillus longan, also known as longan, is the arillus longan of sapindus. Harvesting mature fruits in summer and autumn, drying, and removing shell and kernel. Drying to dryness and sticking no. It is sweet and warm in nature and is produced in various provinces in the south of China. It enters heart and spleen meridians. Has the effects of invigorating heart and spleen, nourishing blood, and tranquilizing mind. Can be used for treating deficiency of both qi and blood, palpitation, amnesia, insomnia, and blood deficiency with sallow complexion.

3. Polygoni Multiflori radix is dried root tuber of Polygonum multiflorum Thunb of Polygonaceae. Collected in autumn and winter when withered, cut off both ends, cleaned, cut into large pieces, and dried. The recipe is the processed product (prepared) of fleece-flower root. Bitter, sweet, astringent and warm in nature. It enters liver, heart and kidney meridians. Has the functions of nourishing liver and kidney, benefiting essence and blood, blackening beard and hair and strengthening bones and muscles. Can be used for treating blood deficiency, sallow complexion, giddiness, tinnitus, premature gray hair, soreness of waist and knees, numbness of limbs, metrorrhagia, leukorrhagia, asthenia due to prolonged illness, and hyperlipidemia, and can be added to make longan tonify heart and strengthen, and supplement liver and kidney.

4. Mulberry, mulberry leaf, mulberry root, mulberry leaf, mulberry. Collected when the fruit turns red in 4-6 months. Dried in the sun or slightly steamed and then dried in the sun. Sweet, sour and cold in nature. It enters heart, liver and kidney meridians. Has the functions of enriching blood, nourishing yin, promoting the production of body fluid and moistening dryness. It can be used for treating vertigo, tinnitus, palpitation, insomnia, premature gray hair, thirst due to body fluid consumption, internal heat, diabetes, blood deficiency, and constipation, and has cold nature, so as to harmonize warm property of radix Polygoni Multiflori, and enhance liver and kidney functions of radix Polygoni Multiflori.

The four medicines have the effects of tonifying heart, kidney, liver and spleen channels, and tonifying heart, kidney, liver and spleen, wherein the heart and kidney are mainly tonifying, and the functions of activating blood circulation and removing blood stasis are realized; animal experiments show that the regulation of the activity of cerebral acetylcholinesterase (AchE) and acetylcholine transferase (chAT) of dementia animal models, the elimination of oxygen radicals, the elimination of aluminum and the like all accord with various theories of theories (cholinergic neurology theory, free radical theory and aluminum poisoning theory) of the pathogenesis of AD, which are proposed by the medical field at present.

Brief introduction of raw materials:

root of red-rooted salvia

Is dried root and rhizome of Salvia Miltiorrhiza bge of Labiatae.

The characteristics are as follows: perennial herbs, long and thin cylindrical roots, purple red, square stems, dense quilts, soft hairs, single feather-shaped compound leaves, 3-4 leaflets; small leaves are oval, sawteeth are arranged on the edges, the two sides are soft hair, the flowers are bluish purple, the head of a raceme or axillary, 2-5 flowers are recurrent, the flower crown is labiate, 2 developing stamens, the fruits are elliptical, the period of the flowers is 5-10 months, the flowers are picked and dug in autumn and winter, cleaned, sliced and dried in the sun.

Nature, flavor and meridian tropism: bitter and slightly cold

The functions and indications are as follows: has effects in promoting blood circulation, dispelling blood stasis, promoting tissue regeneration, clearing away heat, cooling blood, nourishing heart, tranquilizing mind, dredging channels, and stopping bleeding. Modern pharmacological research shows that the salvia miltiorrhiza can improve microcirculation, has a regulating effect on central nerves, can relieve dysphoria and hyperactivity, and is beneficial to regulating neurasthenia, insomnia and dreaminess symptoms of patients.

The application and dosage are as follows: 5-15 g

And (3) storage: and (5) preventing moth in ventilated and dry places.

Polygonum multiflorum Thunb

Is dried root tuber of Polygonum multiflorum Thunb of Polygonaceae. Collected in autumn and winter when withered, cut off both ends, cleaned, cut into large pieces, and dried.

The characteristics are as follows: the product is in the shape of block or irregular spindle, with a length of 6-15 cm and a diameter of 4-12 cm. The surface is reddish brown or reddish brown, has uneven shrinkage, linear furrows, transverse long skin pores and cytoma. The weight and the texture are firm, the fracture is not easy to break, the cross section is light yellow brown or light red brown, the powder is shown, 4-11 similar round special-shaped vascular bundles are annularly arranged at the skin part to form brocade-shaped patterns, the central wood part is larger, and some parts are in the shape of wood cores. Slightly bitter and sweet and astringent in smell.

Content determination: and (4) avoiding light. Measuring by high performance liquid chromatography.

Chromatographic conditions and system applicability test: octadecylsilane chemically bonded silica is used as a filling agent; acetonitrile-water (25:75) as mobile phase; the detection wavelength was 320 nm. The number of theoretical plates is not less than 2000 calculated according to 2.3,5,4' -tetrahydroxystilbene-2-0-beta-D glucoside.

Preparation of a reference solution: accurately weighing appropriate amount of 2,3,5,4' -tetrahydroxystilbene-2-0-beta-D glucoside reference substance, and adding diluted ethanol to obtain solution containing 0.2mg per ml.

Preparing a test solution: weighing 0.2g of the product (sieved by a sieve IV), precisely weighing, placing in a conical flask, precisely adding 25ml of dilute ethanol, weighing, heating and refluxing for 30 minutes, cooling, weighing again, supplementing the lost weight with dilute ethanol, shaking uniformly, and filtering the supernatant with a microporous filter membrane (0.45 μm).

The determination method comprises the following steps: precisely sucking 10 μ l of each of the reference solution and the supply solution, injecting into a liquid chromatograph, and measuring.

Processing: removing impurities, cleaning, slightly soaking, moistening, slicing, and drying.

Nature, flavor and meridian tropism: bitter, sweet, astringent and warm. It enters liver, heart and kidney meridians.

The functions and indications are as follows: detoxication, curing abscess, moistening intestines and relaxing bowels. Used for scrofula, sores and carbuncles, rubella and pruritus, and constipation due to intestinal dryness; hyperlipidemia.

The application and dosage are as follows: 6-12 g.

And (3) storage: placing in dry place for moth proofing.

Prepared fleece flower root (Zhiheshowu)

This herb is a processed product of multiflower knotweed root.

The preparation method comprises the following steps: the preparation method comprises soaking dried radix Polygoni Multiflori slice in water under high pressure in a basket, covering with cloth, soaking for 1-2 hr, soaking for half a day to one day, placing in a steam autoclave, soaking at 121 deg.C under high pressure for 2-10 hr, and drying.

The characteristics are as follows: the product is irregular shrivelled, and has a thickness of about 1 cm. Dark brown or dark brown surface, uneven. Hard, keratotic, tan or black. Light smell, slightly sweet and bitter taste.

Nature, flavor and meridian tropism: bitter, sweet, astringent and warm. It enters liver, heart and kidney meridians.

The functions and indications are as follows: tonify liver and kidney, replenish essence and blood, blacken beard and hair, and strengthen tendons and bones. Can be used for treating blood deficiency, sallow complexion, giddiness, tinnitus, early white beard and hair, soreness of waist and knees, numbness of limbs, metrorrhagia, leukorrhagia, asthenia due to prolonged illness, and hyperlipidemia.

The application and dosage are as follows: 6-12 g.

Longan pulp (Longyanrou)

The product is arillus longan Dimocarpus Longgan Lour of Sapindaceae. Harvesting mature fruits in summer and autumn, drying, and removing shell and kernel. Drying to dryness and sticking no. Longan pulp, longan pulp and fructus Alpinae Oxyphyllae

A trait; longitudinally broken irregular flakes, constant pieces bonded, about 1.5cm long, 2-4 cm wide, about 0.1cm thick, tan, translucent. One side is wrinkled and uneven, the other side is bright and has fine longitudinal wrinkles, the texture is soft and moist, the smell is slight and fragrant, and the taste is sweet.

And (4) checking: moisture, measured by the moisture assay (appendix IX H first method), should not exceed 15.0%

Total ash content: not more than 4.0 percent

Extract: (Chinese pharmacopoeia, appendix XA) at least 70.0%

Nature, flavor and meridian tropism: sweet and warm. It enters heart and spleen meridians.

The functions and indications are as follows: tonify heart and spleen, nourish blood and tranquilize mind. Can be used for treating deficiency of both qi and blood, palpitation, amnesia, insomnia, and blood deficiency with sallow complexion.

The application and dosage are as follows: 9-15 g.

And (3) storage: ventilating, drying, and preventing moisture and moth.

By adopting the technical scheme, the invention has the following technical effects:

the invention is scientifically prepared by traditional Chinese medicines such as salvia miltiorrhiza, longan aril (longan), polygonum multiflorum (prepared), mulberry and the like according to the combination of the traditional Chinese medicine theory, the modern medical theory and the experiment. The preparation method comprises extracting the above materials with water, precipitating with ethanol, concentrating, and making into oral liquid, granule, capsule, and tablet. The composition has effects in invigorating kidney, replenishing essence, promoting blood circulation, dispelling blood stasis, tranquilizing mind, regulating activity of acetylcholinesterase and acetylcholinesterase transferase in cholinergic nervous system of brain, scavenging oxygen free radicals, and removing aluminum. Can be used for preventing and treating amnesia, insomnia, hypomnesis, activity change of cerebral acetylcholinesterase (AchE) and acetylcholine transferase (ChAT), scavenging oxygen free radicals, and removing aluminum due to senile dementia.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, preferred embodiments are given and the present invention is described in further detail. It should be noted, however, that the numerous details set forth in the description are merely for the purpose of providing the reader with a thorough understanding of one or more aspects of the present invention, which may be practiced without these specific details.

The traditional Chinese medicine for preventing and treating senile dementia is prepared from the following traditional Chinese medicine raw materials of active ingredients in parts by weight: 2 parts of salvia miltiorrhiza, 6 parts of longan pulp, 1 part of polygonum multiflorum (prepared) and 7 parts of mulberry.

Processing of Polygonum multiflorum Thunb high pressure method (high pressure method instead of traditional Chinese medicine steaming method, with the advantages of time saving, rapid speed, low energy consumption, low cost, retained effect of original processing method, and suitability for industrial production)

Dried polygonum multiflorum slices → soaked in water to penetrate leap → high pressure in a steam release pressure cooker (105-. The Chinese medicinal composition can be made into oral liquid, granule, capsule and tablet.

Production method and process flow of traditional Chinese medicine oral liquid for preventing and treating senile dementia (1000ml finished product meter)

1. Weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum (prepared), and 3-15 parts of mulberry.

2. Water extraction: the traditional Chinese medicine → the traditional Chinese medicine extraction tank → water with the volume of 10 to 15 times → heating and boiling for 1 to 2 hours → liquid medicine is discharged → the medicine tank is added with water with the volume of 5 to 10 times → reheating and boiling for 0.5 to 1 hour → liquid medicine is discharged and filtered → liquid medicine.

3. Concentrating the liquid medicine: liquid medicine → putting in concentrating tank → heating and concentrating to 2-3 times of medicine volume → cooling

4. Ethanol precipitation: liquid medicine → 3-6 times of 95% ethanol is added until the ethanol concentration reaches 60-75% → stirring and then placing at 4-8 ℃ → standing for 6-48 hours → filtering.

5. And (3) recovering ethanol: filtrate → ethanol recovery tank → heating to recover ethanol → medicinal liquid → filtering after cooling → medicinal liquid.

6. Removing tannin by dripping 3-5% edible gelatin solution into the medicinal liquid while stirring until white precipitate appears, and filtering to remove precipitate.

7. Ethanol precipitation: liquid medicine → 3-6 times of 95% ethanol is added until the ethanol concentration reaches 60-75% → stirring and then placing at 4-8 ℃ → standing for 6-48 hours → filtering.

8. And (3) recovering ethanol: filtrate → ethanol recovery tank → heating to recover ethanol → medicinal liquid → filtering after cooling → medicinal liquid.

9. The medicine is prepared by adding sucrose into the liquid medicine, adding water to 1000ml, and the final concentration of sugar is 20%.

10. Filling into bottles of 10 ml/piece and 100 ml/bottle.

11. Autoclaving at 105 deg.C for 30-40 min.

12. And (6) checking.

13. And (6) packaging to obtain a finished product.

Production method and process flow of traditional Chinese medicine granule for preventing and treating senile dementia (1000g finished product meter)

1. Weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum (prepared), and 3-15 parts of mulberry.

2. Water extraction: the traditional Chinese medicine → the traditional Chinese medicine extraction tank → water with the volume of 10 to 15 times → heating and boiling for 1 to 2 hours → liquid medicine is discharged → the medicine tank is added with water with the volume of 5 to 10 times → reheating and boiling for 0.5 to 1 hour → liquid medicine is discharged and filtered → liquid medicine.

3. Concentrating the liquid medicine: liquid medicine → concentrated in a tank → heated and concentrated to 2-3 times the volume of the medicine → cooled.

4. Ethanol precipitation: liquid medicine → 3-6 times of 95% ethanol is added until the ethanol concentration reaches 60-75% → stirring and then placing at 4-8 ℃ → standing for 6-48 hours → filtering.

5. And (3) recovering ethanol: filtrate → ethanol recovery tank → heating to recover ethanol → medicinal liquid → filtering after cooling → medicinal liquid.

6. Removing tannin by dripping 3-5% edible gelatin solution into the medicinal liquid while stirring until white precipitate appears, and filtering to remove precipitate.

7. Ethanol precipitation: liquid medicine → 3-6 times of 95% ethanol is added until the ethanol concentration reaches 60-75% → stirring and then placing at 4-8 ℃ → standing for 6-48 hours → filtering.

8. And (3) recovering ethanol: filtrate → ethanol recovery tank → heating to recover ethanol → medicinal liquid → filtering after cooling → medicinal liquid.

9. Concentrating the liquid medicine: liquid medicine → concentrated pan → concentrated by heating → to paste.

10. And (3) granulating: paste medicine → adding 940g of cane sugar → granulating by a granulator.

11. Quantitative → packaging → finished product → packaging.

The production method and the process flow of the traditional Chinese medicine capsule for preventing and treating the senile dementia are as follows: (in 222 capsules)

1. Weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum (prepared), and 3-15 parts of mulberry.

2. Water extraction; the traditional Chinese medicine → the traditional Chinese medicine extracting tank → 10 to 15 times of water is added → the heating and boiling is carried out for 1 to 2 hours → the liquid medicine is discharged → the filtration → the medicine residue is added with 5 to 10 times of water → the heating and boiling is carried out for 0.5 to 1 hour → the liquid medicine is discharged → the filtration → the liquid medicine.

3. Concentrating the liquid medicine: liquid medicine → in concentrating tank → heat concentrating → 2-3 times of medicine volume → cooling.

4. Ethanol precipitation: the liquid medicine → 3 to 6 times of 95 percent ethanol is added until the ethanol concentration reaches 60 to 75 percent → stirring → placing at 4 ℃ to 8 ℃ for standing for 6 to 48 hours → filtering → the filtrate.

5. And (3) recovering ethanol: the above filtrate → in the ethanol recovery tank → ethanol is recovered by heating → medicinal liquid → cooling → filtering → medicinal liquid.

6. Removing tannin by dripping 3-5% edible gelatin solution into the medicinal liquid while stirring until white precipitate appears, and filtering to remove precipitate.

7. Ethanol precipitation: liquid medicine → 3-6 times of 95% ethanol is added until the ethanol concentration reaches 60-75% → stirring and then placing at 4-8 ℃ → standing for 6-48 hours → filtering.

8. And (3) recovering ethanol: filtrate → ethanol recovery tank → heating to recover ethanol → medicinal liquid → filtering after cooling → medicinal liquid.

9. Concentrating the liquid medicine: the filtrate → concentrated in a pot → concentrated by heating → to paste.

10. Preparing medicinal powder: the paste medicine → adding auxiliary material → pulverizing and drying → mixing.

11. Preparing capsules and sealing the cans; the medicine pot → capsule machine for capsule manufacturing → can sealing machine for can sealing → finished product.

The production method and the process flow of the traditional Chinese medicine tablet for preventing and treating the senile dementia are as follows:

1. weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum (prepared), and 3-15 parts of mulberry.

2. Water extraction: the traditional Chinese medicine → the traditional Chinese medicine extraction tank → 10 to 15 times of water is added → the heating and boiling is carried out for 1 to 2 hours → the liquid medicine is discharged → the filtration → the medicine residue is added with 5 to 10 times of water → the heating and boiling is carried out for 0.5 to 1 hour → the liquid medicine is discharged → the filtration → the liquid medicine.

3. Concentrating the liquid medicine: liquid medicine → putting in concentrating tank → heating and concentrating → 2-3 times of medicine volume → cooling

4. Ethanol precipitation: adding 3-6 times of 95% ethanol till the ethanol concentration reaches 60-75% → stirring → placing at 4 ℃ -8 ℃ for standing for 6-48 hours → filtering

5. And (3) recovering ethanol: the above filtrate → in the ethanol recovery tank → ethanol is recovered by heating → medicinal liquid → cooling → filtering → medicinal liquid.

6. Removing tannin by dripping 3-5% edible gelatin solution into the medicinal liquid while stirring until white precipitate appears, and filtering to remove precipitate.

7. Ethanol precipitation: liquid medicine → 3-6 times of 95% ethanol is added until the ethanol concentration reaches 60-75% → stirring and then placing at 4-8 ℃ → standing for 6-48 hours → filtering.

8. And (3) recovering ethanol: filtrate → ethanol recovery tank → heating to recover ethanol → medicinal liquid → filtering after cooling → medicinal liquid.

9. Concentrating the liquid medicine: the filtrate → concentrated in a pot → concentrated by heating → to paste.

10. Preparing medicinal powder: the paste medicine → adding auxiliary material → pulverizing and drying → mixing.

11. And (3) tabletting and packaging: the medicine is added → the tablet making machine is used for making tablets → the can sealing machine is used for sealing cans → the finished product is obtained.

In all preparation methods of oral liquid, granule, capsule and tablet, the fleece-flower root needs to be processed by a high pressure method, dried fleece-flower root tablet → water-soaked hydrophilic leap → high pressure in a steam-releasing autoclave (105-.

Brief introduction of the experiment:

1. results of animal treatment trials

The results of the activity assays of cerebral acetylcholine transferase (chAT), acetylcholinesterase (AchE) and normal control group of animal models of dementia mice (no treatment), treatment group and normal control group were compared with the memory after treatment.

TABLE 1 brain AchT (ChAT), AchE (AchE) Activity, memory (maze test)

Analysis of variance, wherein P is less than 0.01 in comparison among groups, and the difference has statistical significance;

the research group of the subject shows that the cerebral neuron of the mouse animal model group with dementia is damaged (pathological morphological microscopic examination) to cause the obvious reduction of the activity of cerebral acetylcholine transferase (chAT) and the activity of acetylcholine esterase (AchE) (biochemical determination), and has obvious difference compared with the normal group through statistics (processing by spread-13 software) to cause the reduction of the level of neurotransmitter acetylcholine, and the treatment group treated by the product has the obvious increase (recovery) of the activity of cerebral acetylcholine transferase (chAT) and acetylcholine esterase (AchE) and is close to the normal group; the memory of each group of mice is measured by a maze test, the memory of the dementia mice is obviously reduced, and the dementia mice are obviously recovered after treatment.

2. Results of clinical treatment trials

TABLE 2 comparison of sleep, memory, and cognitive abilities before and after treatment of dementia patients

As a result, the improvement was significant.

The medicine (oral liquid) is taken 3 times a day, 10mL each time, 30 days as a treatment course, 15 days are separated, the second treatment course is taken, the illness state is obviously improved after the treatment, and the sleep, the memory and the cognitive ability are all obviously improved.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.

Claims (8)

1. A traditional Chinese medicine for preventing and treating senile dementia is characterized in that: the traditional Chinese medicine composition is prepared from the following traditional Chinese medicine raw materials in parts by weight: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry.

2. The traditional Chinese medicine for preventing and treating senile dementia according to claim 1, which is characterized in that: can be made into oral liquid, granule, capsule and tablet.

3. A production method of a traditional Chinese medicine oral liquid for preventing and treating senile dementia is characterized in that: the production method of the oral liquid comprises the following steps:

step 1: weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry;

step 2: putting the raw materials in the step 1 into a traditional Chinese medicine extraction tank, adding 10-15 times of weight of water, heating and boiling for 1-2 hours, discharging liquid medicine, then adding 5-10 times of weight of water into the medicine tank, heating and boiling again for 0.5-1 hour, discharging the liquid medicine, converging and filtering to obtain liquid medicine;

and step 3: putting the liquid medicine into a concentration tank, heating and concentrating to 0.2-0.3 time of volume, and cooling the concentrated liquid medicine;

and 4, step 4: adding 90-95% ethanol with volume of 3-6 times of the cooled concentrated liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a filtrate;

and 5: putting the filtrate into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain an initial liquid medicine, cooling the initial liquid medicine, and filtering to obtain a filtered liquid medicine;

step 6: dripping 3-5% edible gelatin solution into the filtered liquid medicine while stirring, stopping stirring until white precipitate appears, and filtering to remove precipitate to obtain tannin-removed liquid medicine;

and 7: adding 95% ethanol with volume of 3-6 times of the tannin-removed liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a secondary filtrate;

and 8: placing the secondary filtered liquid medicine into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain secondary liquid medicine, cooling the secondary liquid medicine, and filtering to obtain secondary filtered liquid medicine;

and step 9: adding sucrose into the twice-filtered medicinal liquid, adding water to 1000ml, and obtaining oral medicinal liquid with sugar concentration of 20%;

step 10: filling the oral liquid medicine, autoclaving the filled liquid medicine, then inspecting, and packaging to obtain the finished product.

4. The method for producing a Chinese medicinal oral liquid for preventing and treating senile dementia according to claim 3, wherein the method comprises the following steps: in the step 1, the polygonum multiflorum is processed by a high pressure method to obtain the prepared polygonum multiflorum, the process is that the dried polygonum multiflorum slices are soaked in water and are wet, the dried polygonum multiflorum slices are put into a steam pressure cooker at the temperature of 105 ℃ and 121 ℃ for 2-10 hours, and then the polygonum multiflorum slices are taken out and dried to obtain the prepared polygonum multiflorum.

5. The method for producing a Chinese medicinal oral liquid for preventing and treating senile dementia according to claim 3, wherein the method comprises the following steps: in the step 10, during filling, 10 ml/bottle and 100 ml/bottle are sterilized under high pressure at 105 ℃ for 30-40 minutes.

6. A production method of traditional Chinese medicine granule for preventing and treating senile dementia is characterized in that: the production method of the granule comprises the following steps,

step 1: weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry;

step 2: putting the raw materials in the step 1 into a traditional Chinese medicine extraction tank, adding 10-15 times of weight of water, heating and boiling for 1-2 hours, discharging liquid medicine, then adding 5-10 times of weight of water into the medicine tank, heating and boiling again for 0.5-1 hour, discharging the liquid medicine, converging and filtering to obtain liquid medicine;

and step 3: putting the liquid medicine into a concentration tank, heating and concentrating to 0.2-0.3 time of volume, and cooling the concentrated liquid medicine;

and 4, step 4: adding 90-95% ethanol with volume of 3-6 times of the cooled concentrated liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a filtrate;

and 5: putting the filtrate into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain an initial liquid medicine, cooling the initial liquid medicine, and filtering to obtain a filtered liquid medicine;

step 6: dripping 3-5% edible gelatin solution into the filtered liquid medicine while stirring, stopping stirring until white precipitate appears, and filtering to remove precipitate to obtain tannin-removed liquid medicine;

and 7: adding 95% ethanol with volume of 3-6 times of the tannin-removed liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a secondary filtrate;

and 8: placing the secondary filtered liquid medicine into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain secondary liquid medicine, cooling the secondary liquid medicine, and filtering to obtain secondary filtered liquid medicine;

and step 9: heating and concentrating the secondary filtered liquid medicine in a concentrating pan to obtain pasty medicine;

step 10: and (3) adding 940g of cane sugar into the pasty medicine, then putting the pasty medicine into a granulator for granulation to obtain medicine granules, and quantitatively packaging the medicine granules to obtain a finished product.

7. A production method of a traditional Chinese medicine capsule for preventing and treating senile dementia is characterized in that: the production method of the capsule comprises the following steps:

step 1: weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry;

step 2: putting the raw materials in the step 1 into a traditional Chinese medicine extraction tank, adding 10-15 times of weight of water, heating and boiling for 1-2 hours, discharging liquid medicine, then adding 5-10 times of weight of water into the medicine tank, heating and boiling again for 0.5-1 hour, discharging the liquid medicine, converging and filtering to obtain liquid medicine;

and step 3: putting the liquid medicine into a concentration tank, heating and concentrating to 0.2-0.3 time of volume, and cooling the concentrated liquid medicine;

and 4, step 4: adding 90-95% ethanol with volume of 3-6 times of the cooled concentrated liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a filtrate;

and 5: putting the filtrate into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain an initial liquid medicine, cooling the initial liquid medicine, and filtering to obtain a filtered liquid medicine;

step 6: dripping 3-5% edible gelatin solution into the filtered liquid medicine while stirring, stopping stirring until white precipitate appears, and filtering to remove precipitate to obtain tannin-removed liquid medicine;

and 7: adding 95% ethanol with volume of 3-6 times of the tannin-removed liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a secondary filtrate;

and 8: placing the secondary filtered liquid medicine into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain secondary liquid medicine, cooling the secondary liquid medicine, and filtering to obtain secondary filtered liquid medicine;

and step 9: heating and concentrating the secondary filtered liquid medicine in a concentrating pan to obtain pasty medicine;

step 10: adding adjuvants into the paste, pulverizing, drying, mixing, making into capsule, and sealing with a can sealing machine to obtain the final product.

8. A production method of a traditional Chinese medicine tablet for preventing and treating senile dementia is characterized in that: the production method of the tablet comprises the following steps:

step 1: weighing the medicines: 1-3 parts of salvia miltiorrhiza, 3-15 parts of longan pulp, 0.5-2 parts of polygonum multiflorum and 3-15 parts of mulberry;

step 2: putting the raw materials in the step 1 into a traditional Chinese medicine extraction tank, adding 10-15 times of weight of water, heating and boiling for 1-2 hours, discharging liquid medicine, then adding 5-10 times of weight of water into the medicine tank, heating and boiling again for 0.5-1 hour, discharging the liquid medicine, converging and filtering to obtain liquid medicine;

and step 3: putting the liquid medicine into a concentration tank, heating and concentrating to 0.2-0.3 time of volume, and cooling the concentrated liquid medicine;

and 4, step 4: adding 90-95% ethanol with volume of 3-6 times of the cooled concentrated liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a filtrate;

and 5: putting the filtrate into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain an initial liquid medicine, cooling the initial liquid medicine, and filtering to obtain a filtered liquid medicine;

step 6: dripping 3-5% edible gelatin solution into the filtered liquid medicine while stirring, stopping stirring until white precipitate appears, and filtering to remove precipitate to obtain tannin-removed liquid medicine;

and 7: adding 95% ethanol with volume of 3-6 times of the tannin-removed liquid medicine until the ethanol concentration reaches 60-75%, stirring, standing in an environment of 4-8 ℃ for 6-48 hours, and filtering to obtain a secondary filtrate;

and 8: placing the secondary filtered liquid medicine into an ethanol recovery tank, heating the recovery tank to recover ethanol to obtain secondary liquid medicine, cooling the secondary liquid medicine, and filtering to obtain secondary filtered liquid medicine;

and step 9: heating and concentrating the secondary filtered liquid medicine in a concentrating pan to obtain pasty medicine;

step 10: adding adjuvants into the paste medicine, pulverizing, drying, mixing, tabletting, and canning to obtain the final product.