CN111603550B

CN111603550B - Composition containing wheat oligopeptide and application thereof

Info

Publication number: CN111603550B
Application number: CN202010607551.XA
Authority: CN
Inventors: 张九勋; 张学军; 田明展; 张西平; 魏玮; 苏鹏
Original assignee: ZHONGSHI DUQING (SHANDONG) BIOTECH CO Ltd
Current assignee: ZHONGSHI DUQING (SHANDONG) BIOTECH CO Ltd
Priority date: 2020-06-29
Filing date: 2020-06-29
Publication date: 2022-09-06
Anticipated expiration: 2040-06-29
Also published as: CN111603550A

Abstract

The invention provides a composition containing wheat oligopeptide and application thereof, belonging to the technical field of sleep improvement, and comprising the wheat oligopeptide, casein peptide and leguminous peptide, wherein the mass ratio of the wheat oligopeptide, the casein peptide and the leguminous peptide is (35-45): (30-40): (20-30). Compared with single use, the composition provided by the invention obviously improves the sleep quality, and the improvement effect reaches 119%.

Description

Composition containing wheat oligopeptide and application thereof

Technical Field

The invention belongs to the technical field of sleep improvement, and particularly relates to a composition containing wheat oligopeptide and application thereof.

Background

Sleep is an important physiological phenomenon. Good sleep plays an important role in maintaining normal physiological activities and physical and mental health. The insomnia brings trouble to physical and mental health of patients, makes the circadian rhythm of people disordered, reduces the activity of the people and seriously influences the life quality of the patients.

Sleep is also a metabolic process of memory cells: the aged cells will be input for each permutation of memory information into new cells for storage. These include sports, language areas, balance keys, and some past events and memories in daily life. They are both substances, so they are also present in substance form.

If a person is in a state of sleep for a long time, the memory cells cannot live healthily, and some health problems, even diseases, such as aphasia, spasm, or forced sleep caused by shock and syncope, can easily occur. Canceration is also easily produced over time. Regular life is a good foundation, but long-term "regular" fatigue is dangerous. Scientists suggest that economically competent persons are best on their own without compromising their health in order to advocate ethics. According to the latest published sleep survey data of the Chinese sleep research society, the incidence rate of insomnia of Chinese is 38.2 percent.

At present, a plurality of physical sleep-assisting products are available in the market, but the products are used for assisting sleep and are not used for treating symptoms and root causes, and after the products are adapted, the products cannot promote sleep of people. Such as intelligent mattresses, intelligent bulbs, eye masks, voice imitators.

There are also drugs on the market for treating insomnia, mainly sedatives, antidepressants, antihistamines, hypnotics, etc. The tranquilizer has problems of addiction and dependence after long-term administration, and once dependence is formed, the tranquilizer cannot be left and used as essential thing in life, and if the tranquilizer is not used, the tranquilizer is difficult to fall asleep or fall asleep all night. Not only is highly stressed due to drug shortage, but also has a feeling of general discomfort, and a combination of physiological, emotional, behavioral and cognitive symptoms appears. Clinical observation of some medical scientists shows that people who often take hypnotic drugs gradually increase the dosage of the drugs, which is obviously more than normal people, and later, people who take hypnotic drugs cannot achieve the purpose of improving sleep, but become excited and euphoric, gait instability, unclear mouth and teeth, and some symptoms such as absentmindedness and the like. It is proved that people who take hypnotics for a long time are very easy to have hypnotic dependence. Long-term administration of hypnotic can reduce memory and intelligence. This is even more evident in the elderly. Physicians have also found that frequent administration of hypnotics by persons over the age of 60 directly affects the ability of the brain to balance and maintain a clear head, giving them a risk of falling and fracture over time. Foreign research also shows that long-term administration of hypnotics has a certain relationship with the onset of senile dementia. The sleep caused by taking hypnotic is not completely the same as normal sleep. The sleepiness is often aversion to dreams, and the phenomena of timed early awakening and daytime sleepiness are caused, so that the physical strength and the energy recovery are not good. Character emotion changes. Some people addicted to hypnotics also gradually change their characters, become emotional fright, or have violent spleen qi, which is usually caused by petiolence, selfishness and consolidation.

Because the medicine can damage the body, the health food for non-medicine treatment becomes more and more choices for insomnia people, and a plurality of health food for improving sleep in the market are added with some expensive Chinese medicinal materials such as ginseng and the like, so that the cost is high, the effect is not fully exerted, and the common effect or the ineffective effect is caused. And the required medicines are more in types, so that the preparation method is complex, the extraction of multiple components is incomplete, the loss of the components is caused, and the sleep-aiding function of the health-care food is ineffective.

In order to solve the problem of insomnia masses, the invention provides a health food for improving sleep and a preparation method thereof. The food disclosed by the invention is safe to eat, free of toxic and side effects, balanced in raw material matching, capable of treating insomnia and good in effect on improving sleep as proved by human experience experiments and animal mice experiments.

Disclosure of Invention

In view of the above, the present invention aims to provide a composition containing wheat oligopeptide and an application thereof, wherein the composition provided by the present invention significantly improves sleep compared to single use.

In order to achieve the above object, the present invention provides the following technical solutions:

the invention provides a composition containing wheat oligopeptide, which comprises 35-45 mass parts of wheat oligopeptide, 30-40 mass parts of casein peptide and 20-30 mass parts of soybean peptide.

Preferably, the mass ratio of the wheat oligopeptide, the casein peptide and the soybean peptide is 40:36: 24.

Preferably, the wheat oligopeptide contains amino acid sequences shown as SEQ ID No.1 and SEQ ID No. 2.

Preferably, the casein peptide contains amino acid sequences shown as SEQ ID No.3 and SEQ ID No. 4.

Preferably, the content of nicotinamide mononucleotide in the soybean peptide is 43 mg/g.

The invention also provides application of the composition in the technical scheme in preparation of a medicament for tranquilizing and allaying excitement.

The invention also provides application of the composition in the technical scheme in preparing a medicine for improving sleep.

The invention provides a composition containing wheat oligopeptide, which comprises the wheat oligopeptide, casein peptide and leguminous peptide, wherein the mass ratio of the wheat oligopeptide to the casein peptide to the leguminous peptide is (35-45) to (30-40) to (20-30). Compared with single use, the composition provided by the invention obviously improves the sleep quality, and the improvement effect reaches 119%. In the invention, the wheat oligopeptide, the casein peptide and the soybean peptide have the functions of tranquilizing and allaying excitement, and the quality of sleep is obviously improved after the wheat oligopeptide, the casein peptide and the soybean peptide are combined.

Drawings

FIG. 1 shows the sleep improvement effect of the test sample on the insomnia of zebra fish;

FIG. 2 shows the sleep improvement effect (insomnia time) of the test article on insomnia of zebra fish.

Detailed Description

The invention provides a composition containing wheat oligopeptide, which comprises wheat oligopeptide, casein peptide and soybean peptide, wherein the mass ratio of the wheat oligopeptide to the casein peptide to the soybean peptide is (35-45): (30-40): (20-30); the mass ratio of the wheat oligopeptide, the casein peptide and the soybean peptide is preferably 40:36: 24.

In the invention, the wheat oligopeptide contains amino acid sequences shown as SEQ ID No.1 and SEQ ID No.2, and the amino acid sequences are as follows:

SEQ ID No.1：APIASLLPPY；

SEQ ID No.2：KLPPVGPY。

in the invention, APIASLLPPY and KLPPVGPY in the wheat oligopeptide have the effects of tranquilizing and allaying excitement and improving sleep. In the invention, the wheat oligopeptide is purchased from the product sold by Zhongshidu Qing biotechnology limited company, and the product model is 200314001.

In the invention, the casein peptide contains amino acid sequences shown as SEQ ID No.3 and SEQ ID No.4, and the amino acid sequences are as follows:

SEQ ID No.3：QYPTQPPQ；

SEQ ID No.4：VGNAPTY。

in the present invention, QYPTQPPQ and VGNAPTY among the casein peptides have an effect of improving sleep. In the invention, the casein peptide is purchased from Zhongshidu Qing Biotechnology GmbH with the product model number of 200507001.

In the present invention, the content of nicotinamide mononucleotide in said green soybean peptide is preferably 43 mg/g. In the invention, the green soy peptide has the effects of tranquilizing and allaying excitement and improving sleep. In the invention, the soybean peptide is purchased from Zhongshidu Qing Biotechnology Co., Ltd, and the product model is 200301001.

The preparation method of the composition containing the wheat oligopeptide is not particularly limited, and the wheat oligopeptide, the casein peptide and the soybean peptide are mixed.

In the present invention, the method of using the composition preferably comprises: the composition is mixed with warm water and administered 3 times a day, wherein the ratio of water volume to composition mass is 150ml:5 g.

The invention also provides application of the composition in the technical scheme in preparation of a medicament for tranquilizing and allaying excitement. The dosage form of the drug is not particularly limited in the present invention, and a pharmaceutically acceptable dosage form of the composition may be employed.

The invention also provides application of the composition in the technical scheme in preparation of a medicine for improving sleep. The dosage form of the drug is not particularly limited in the present invention, and a pharmaceutically acceptable dosage form of the composition may be employed.

The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.

Example 1

Mixing wheat oligopeptide, casein peptide and soybean peptide to obtain a composition, wherein the mass ratio of the wheat oligopeptide to the casein peptide to the soybean peptide is 40:36:24, the wheat oligopeptide contains amino acid sequences shown as SEQ ID No.1 and SEQ ID No.2, the casein peptide contains amino acid sequences shown as SEQ ID No.3 and SEQ ID No.4, and the content of nicotinamide mononucleotide in the soybean peptide is 43 mg/g.

Example 2

Randomly selecting 5dpf wild type AB series zebra fish in a 6-well plate, inducing the zebra fish by caffeine to establish an insomnia model, respectively performing water-soluble administration on wheat peptide oligopeptide, casein peptide, phaseolamin and the composition (namely tripeptide complex peptide) prepared in example 1 (500 mug/mL, 500 mug/mL and 500 mug/mL) in water, and positive control medicament lithium chloride with the concentration of 500 mug M, and setting a normal control group (water for fish culture) and a model control group at the same time, wherein the capacity of each well (experimental group) is 3 mL. After the test sample is treated for 18h, the zebra fish is transferred to a 96-well plate, 1 tail is placed in each hole (namely each concentration group), 10 zebra fish are randomly selected from each experiment group, a behavior analyzer is used for measuring the insomnia time of the zebra fish, and the sleep improvement effect of the test sample on caffeine-induced insomnia of the zebra fish is evaluated according to the insomnia time. The calculation formula of the sleep improvement effect of the test sample on the insomnia zebra fish is as follows:

statistical analysis was performed using analysis of variance and Dunnett's t-test, with p <0.05 indicating significant differences, providing a representative experimental profile. The results are shown in Table 1 and FIGS. 1-2.

Table 1 quantitative data of the test article for sleep improvement of zebra fish (n ═ 10)

The wheat oligopeptide, the composition, the green soy peptide and the casein peptide which can be obtained from the table 1 and the figures 1-2 have obvious sleep improvement effect on caffeine-induced insomnia of the zebra fish, and the improvement effect after the three are compounded is obviously stronger than that of the wheat oligopeptide, the composition, the green soy peptide and the casein peptide which are used alone.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Sequence listing

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Claims

1. The application of the composition containing the wheat oligopeptide in preparing the medicine for improving insomnia;

the composition consists of wheat oligopeptide, casein peptide and soybean peptide, wherein the mass ratio of the wheat oligopeptide to the casein peptide to the soybean peptide is (35-45): (30-40): (20-30);

the wheat oligopeptide has short peptides with amino acid sequences shown as SEQ ID No.1 and SEQ ID No. 2;

the casein peptide has short peptides with amino acid sequences shown as SEQ ID No.3 and SEQ ID No. 4;

the content of nicotinamide mononucleotide in the green soybean peptide is 43 mg/g;

the wheat oligopeptide is purchased from a product sold by Zhongshidu Qing biotechnology limited company, and the model of the product is 200314001;

the casein peptide is purchased from Zhongshidu Qing Biotechnology GmbH, and the product model is 200507001;

the soybean peptide is purchased from Zhongshi Duqing Biotechnology Co., Ltd, and the product model is 200301001.

2. The use of claim 1, wherein the mass ratio of wheat oligopeptide, casein peptide and soy peptide is 40:36: 24.