CN111603517B

CN111603517B - Application of radix Lespedezae Cuneatae in preparing medicine for relieving cough and eliminating phlegm

Info

Publication number: CN111603517B
Application number: CN202010551977.8A
Authority: CN
Inventors: 高渐飞; 周玮; 刘中华; 孙燕
Original assignee: GUIZHOU INSTITUTE OF MOUNTAINOUS RESOURCE
Current assignee: GUIZHOU INSTITUTE OF MOUNTAINOUS RESOURCE
Priority date: 2020-06-17
Filing date: 2020-06-17
Publication date: 2022-11-18
Anticipated expiration: 2040-06-17
Also published as: CN111603517A

Abstract

The invention discloses an application of a kadsura root in preparing a medicine for relieving cough and eliminating phlegm. According to the invention, the root of the plant of the clod is subjected to a mouse experiment, and the result shows that the clod has obvious effects of inhibiting cough, curing cough and eliminating phlegm, and can be used as a cough-relieving and phlegm-eliminating medicine; the invention explores the new medicinal application of the root of the Chinese medicinal herb rice ball in the aspects of relieving cough and eliminating phlegm, provides a new choice of medicaments for relieving cough and eliminating phlegm for patients, also widens the medicinal value and the application of the medicinal material rice ball, exploits a new application field of the Chinese medicinal herb rice ball, and has wide application prospect and good economic benefit and social benefit.

Description

Application of rhizoma Phragmitis in preparing medicine for relieving cough and eliminating phlegm

Technical Field

The invention relates to a new application of a root of a rice ball in preparing a medicine for relieving cough and eliminating phlegm, belonging to the technical field of medicines.

Background

Kadsura coccinea (lem.) A.C.Smith) ], also known as Kadsura coccinea, is a plant of Kadsura of the family Magnoliaceae, and is a perennial common woody vine; mainly distributed in Guizhou, hunan, yunnan, sichuan, guangxi, guangdong, etc. The traditional Chinese medicine composition is used for treating rheumatic arthralgia, activating blood and dissolving stasis, promoting qi circulation and relieving pain, acute and chronic rheumatic arthritis, rheumatoid arthritis and scapulohumeral periarthritis. From the aspect of the utilized functions, no relevant report is found about the application of the lump prepared from the fringed rice in treating cough relieving and phlegm eliminating.

Disclosure of Invention

The invention aims to provide the application of the root of the rice ball in preparing the medicine for relieving cough and eliminating phlegm, provide a new application approach for the medicinal value of the rice ball and provide a new medicine choice for consumers.

In order to solve the technical problems, the technical scheme of the invention is as follows:

application of radix Campylotropis Hirtella in preparing medicine for relieving cough and eliminating phlegm is provided.

The application of the root of the kadsura coccinea in preparing the medicine for relieving cough and eliminating phlegm is a preferable scheme, and the medicine is prepared into a clinically common medicinal preparation by taking the root of the kadsura coccinea as an active ingredient and pharmaceutically common auxiliary materials according to a conventional method.

The above-mentioned application of the root of the Boschniakia chinensis in preparing the medicament for relieving cough and eliminating phlegm, specifically, the pharmaceutical preparation can be a dosage form of being administrated through gastrointestinal tract, including tablets, capsules, granules, powder, solutions, emulsions or dropping pills.

The application of the kadsura root in preparing the medicine for relieving cough and eliminating phlegm is specifically that the medicine preparation can also be in a parenteral administration dosage form, including injection, spray, aerosol, patch, paste, drops or gargle.

The invention has the beneficial effects that: compared with the prior art, the invention explores the new medicinal application of the rhizoma typhonii in the aspects of relieving cough and eliminating phlegm, has obvious curative effect and provides a new choice of cough-relieving and phlegm-eliminating medicaments for patients. Meanwhile, the invention also widens the medicinal value and application of the medicinal material of the cold rice ball, has important significance for promoting the cold rice ball to prepare the cough-relieving and phlegm-eliminating medicament, develops a new application field of the cold rice ball, and has wide application prospect and good economic benefit and social benefit.

The present invention will be further described with reference to the following embodiments.

Detailed Description

Example 1: preparing crude drugs of the kadsura root: cleaning root of the plant of the Bombaria smith, air drying, and pulverizing to obtain crude drug of Bombaria smith; collecting 180g of Boschniakia chinensis, adding 1000mL of distilled water, decocting for 2h, filtering to remove residue, concentrating into extract, and storing in refrigerator at 4 deg.C. When in use, the medicine is diluted to low, medium and high dosage by sodium carboxymethyl cellulose.

1. Cough relieving effect experiment:

the prepared crude drug of the cooked rice ball is subjected to a mouse experiment, and the specific experimental process comprises the following steps:

(1) After preheating the ultrasonic atomizer, adding 50 mL of ammonia water with 13% mass percentage concentration; the mouse is placed into a 1000mL beaker, the mouth of the beaker is sealed by a preservative film, two openings are formed, one opening is used for inserting an outlet pipe of the ultrasonic atomizer, and the other opening is a small ventilation hole.

(2) And (3) continuously introducing ammonia gas into the ultrasonic atomizer for 60s at the maximum spraying force (MAX), immediately closing the ultrasonic atomizer, and quickly opening the preservative film to ensure that the ammonia gas in the beaker is naturally diffused. While passing ammonia gas, the number of coughs (manifestation of cough: contraction of mouse abdominal muscle with mouth widening and cough sound) and the cough latency (time to first onset of cough) were observed and recorded for 3 min.

(3) Selecting 60 mice which are qualified in screening (standard: all people with cough latency less than 15s or more than 50s are eliminated, and all people with cough frequency less than 10 times or more than 80 times within 3min are eliminated), randomly dividing the mice into 5 groups, and each group comprises 12 mice; respectively weaving as follows: distilled water group, lung power cough mixture group, low dose group of the rice cooker, medium dose group of the rice cooker and high dose group of the rice cooker, the lung power cough mixture is a Chinese patent medicine produced by Guizhou Jianxing pharmaceutical industry limited company, and the main treatment is as follows: clear heat and remove toxicity, relieve cough and eliminate phlegm. And (3) continuously intragastrically irrigating the mice of each group for 14d by using corresponding medicaments respectively, wherein the administration volume is 20mL/kg and 1 time/d, the model is made 1h after the last administration, and the cough frequency and the cough latency time of the mice within 3min are observed and recorded.

2. An expectorant effect experiment:

(1) Weighing 0.05g of phenol red, dissolving the phenol red by NaOH with the concentration of 1mol/L, adding physiological saline with the pH value of 8.95 to 100mL and the concentration of 500.00ug/mL, then diluting the phenol red into phenol red solutions with the concentrations of 250.00ug/mL, 125.00ug/mL, 62.50ug/mL and 31.25 ug/mL step by step, and measuring the absorbance at 546 nm.

(2) Selecting 60 mice which are qualified in screening (standard: all people with cough latency less than 15s or more than 50s are eliminated, and all people with cough frequency less than 10 times or more than 80 times within 3min are eliminated), randomly dividing the mice into 5 groups, and each group comprises 12 mice; respectively weaving as follows: the mice in each group are continuously gavaged with corresponding medicines for 14 days respectively, the administration volume is 20mL/kg,1 time/d, after 30min of the last administration, the mice are subjected to intraperitoneal injection of 0.5% phenol red for 20mL/kg, and the mice are killed by air embolism after 1 h.

(3) Fixing the mouse in supine position on a frog plate, isolating trachea (preparing 5mL numbered test tubes in advance, adding 5% NaHCO ₃ Solution 1.5 mL), 5% NaHCO was drawn up with a 1 mL syringe ₃ 0.5 mL of the solution was injected into the trachea of the mouse, the respiratory tract was repeatedly flushed back and forth 3 times, and finally 1 time the flushing solution was collected in the corresponding numbered test tube and repeated 2 more times as described above. The combined washing solutions were centrifuged (rotation speed 1000rpm,5 min), and the supernatant was collected and measured for absorbance at 546 nm. The concentration was calculated according to the phenol red standard curve regression equation.

3. Statistical table:

TABLE 1 statistical table of cough incubation period, cough frequency and phenol red excretion of mice

Group of	Dosage per g.kg ^-1	Cough latency/s	Cough times within 3min	Phenol Red excretion (ug/kg)
					Distilled water set	20	35	122.5	0.234
Lung-power cough mixture group	10	47.8	97.8	0.27
					Low dose group of root of Kadsura coccinea	5	50.8	85.3	0.25
Middle-dose group of the root of Kadsura coccinea	10	55	85.8	0.257
					High-dose group of root of Kadsura coccinea	20	63.5	78	0.278

As shown in Table 1, the root of the Kadsura coccinea can inhibit the occurrence of cough in mice, the latency period is respectively increased by 45.1 to 81.4 percent compared with a distilled water group, and the latency period is respectively increased by 6.3 to 32.8 percent compared with an antitussive lung-power cough mixture group; the radix glehniae has an influence on mouse phenol red excretion, the phenol red excretion is increased by 6.8 to 18.8 percent compared with a distilled water group, and the high-dose group is increased by 3.0 percent compared with an antitussive lung-potency cough mixture group, so that the radix glehniae has obvious effects on suppressing cough (prolonging cough latency), curing cough (reducing cough frequency) and eliminating phlegm (increasing mouse tracheal phenol red excretion), wherein the effect of the high-dose group is far better than that of the antitussive lung-potency cough mixture.

The embodiments of the present invention are not limited to the above-described examples, and various changes made without departing from the spirit of the present invention are within the scope of the present invention. Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, while the invention has been described in detail with reference to the above-mentioned preferred examples, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.

Claims

1. The application of the root of the Botrychium hybridum in preparing the medicine for relieving cough and eliminating phlegm is to prepare the clinically common medicinal preparation by taking the root of the Botrychium hybridum as an active ingredient and adding the pharmaceutically common auxiliary materials according to a conventional method.

2. Use of a root of a lump of fringed rice according to claim 1 for the preparation of a medicament for the treatment of cough suppressing expectoration, characterized in that: the medicine preparation is a gastrointestinal administration preparation, and comprises tablets, capsules, granules, powder, solutions, emulsions or dripping pills.

3. Use of a root of a lump of fringed rice according to claim 1 for the preparation of a medicament for the treatment of cough suppressing expectoration, characterized in that: the pharmaceutical preparation is a parenteral administration dosage form, and comprises injection, spray, aerosol, patch, paste, drop or gargle.