CN111575182A - Cell culture device with temperature monitoring function and temperature monitoring and regulating method - Google Patents
Cell culture device with temperature monitoring function and temperature monitoring and regulating method Download PDFInfo
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Abstract
The invention discloses a cell culture device with a temperature monitoring function, which comprises a microfluidic chip, a light source, a convex lens, a lens bracket and an image sensor, wherein the microfluidic chip is provided with a temperature change channel, and a temperature change material is filled in the temperature change channel. The invention also discloses a temperature monitoring and regulating method for cell culture, which comprises the steps of fixing the microfluidic chip and the image sensor, opening the light source system, introducing the cultured cells and cell culture solution, judging the current cell culture temperature by analyzing the gray values of RGB three channels of the temperature-change image, correspondingly adjusting the cell culture temperature, and carrying out real-time monitoring and regulating of cell culture.
Description
Technical Field
The invention belongs to the technical field of cell culture, relates to a cell culture device with a temperature monitoring function, and further relates to a temperature monitoring and regulating method for cell culture.
Background
The cell culture technology is an important and common technology in cell biology research methods, and the temperature control is particularly important for the culture of cells because the temperature must be constant and proper to maintain the vigorous growth of cultured cells. The traditional temperature monitoring mode is that cells are cultured in a constant temperature incubator, and the experimental data of the temperature in the incubator needs to be detected and recorded manually in the culturing process, so that high labor cost is consumed, and the risk of unobtrusive detection results also exists.
Disclosure of Invention
The invention aims to provide a cell culture device with a temperature monitoring function, which can realize high-efficiency non-manual monitoring of cell culture temperature.
The invention also aims to provide a temperature monitoring and regulating method for cell culture, which can monitor the cell culture temperature of the microfluidic chip in real time.
The first technical scheme is that the cell culture device with the temperature monitoring function comprises a micro-fluidic chip, a light source and a lens bracket provided with a convex lens, wherein the micro-fluidic chip, the light source and the convex lens are arranged from top to bottom in sequence, the light source and the convex lens are coaxial, a temperature change channel is arranged on one side of a main channel of the micro-fluidic chip, a temperature change material is filled in the temperature change channel, and an image sensor is arranged at the temperature change channel on the lower surface of the micro-fluidic chip.
The first technical solution of the present invention is also characterized in that,
the micro-fluidic chip is a three-fork micro-fluidic chip and is provided with three micro-fluidic channel inlets and one micro-fluidic channel outlet.
The temperature change material is temperature change ink.
The light source is fixed on the light source bracket, the micro-fluidic chip is arranged on the base, the base is fixedly provided with the fixed bracket, and the light source bracket and the lens bracket are fixedly connected with the fixed bracket.
The invention relates to a temperature monitoring and regulating method for cell culture, which is implemented by applying the cell culture device to culture cells according to the following steps:
step 1, fixing the microfluidic chip and the image sensor, turning on a power supply, and connecting the image sensor with a computer CPU (central processing unit) through a USB (universal serial bus) interface;
step 2, turning on a light source system, and adjusting the position of the light source to enable the light source, the convex lens and the image sensor to be on the same axis, so that imaging is clear;
step 4, the computer CPU firstly turns on the image sensor to electrify, preheats the cultured cells and the cell culture solution through the heating of a chip in the image sensor, collects temperature-change images of a temperature-change channel at equal intervals after preheating, and judges the current cell culture temperature by analyzing the gray value of RGB three channels of the temperature-change images;
and 5, correspondingly adjusting the culture temperature of the cells according to the temperature requirement of the cultured cells, and carrying out real-time monitoring and regulation of cell culture.
The second technical solution of the present invention is also characterized in that,
in step 4, the CPU of the computer firstly turns on the image sensor for powering-on and preheating for 25s-35 s.
In the step 4, the gray values of RGB three channels of the temperature-varying cell image are analyzed to judge the current cell culture temperature, specifically, the temperature-varying images of the temperature-varying channels at different temperatures are collected, the gray value histograms of the RGB three channels of the temperature-varying image at different temperatures are counted, the range of the gray values of the RGB three channels corresponding to each temperature value is determined by the histogram, and the temperature value of the current cell culture is correspondingly determined according to the range of the gray values of the RGB three channels of the collected temperature-varying image.
Step 5, specifically, comparing the current cell culture temperature judged in the step 4 with the standard temperature range of the cultured cells by the CPU, if the current cell culture temperature is lower than the lowest threshold value of the standard temperature range of the cultured cells, keeping the image sensor powered on, and continuously increasing the cell culture temperature through the heating of a chip in the image sensor until the cell culture temperature belongs to the standard temperature range of the cultured cells; if the current cell culture temperature is higher than the highest threshold value of the standard temperature range of the cultured cells, the flow rate of the cell culture solution is accelerated by the micropump so that the cell culture temperature is reduced until the cell culture temperature belongs to the standard temperature range of the cultured cells.
The invention has the beneficial effects that:
the cell culture device with the temperature monitoring function has a stable structure, is simple and easy to carry about; the lens-free imaging system is adopted, a uniform parallel surface light source is formed by a point light source and a lens, the diffraction is small, the imaging is clear, and the convex lens plays roles of expanding beam and collimating and does not play a role of imaging; the current cell culture temperature is rapidly determined through the color change of the temperature change material, the cell culture temperature can be monitored in real time, heating or cooling treatment can be correspondingly carried out according to the monitored temperature subsequently, and continuous culture of cells in a required temperature interval is effectively guaranteed.
The temperature monitoring and regulating method for cell culture analyzes the gray values of RGB three channels of temperature-variable cell images of a microfluidic chip at different temperatures by a statistical method, and judges the temperature corresponding to the temperature-variable images acquired in real time according to the histogram information of the gray values; the cell culture temperature of the microfluidic chip can be monitored in real time, the temperature detection speed is high, the accuracy is high, and the labor cost is greatly saved.
Drawings
FIG. 1 is a schematic view showing the structure of a cell culture apparatus having a temperature monitoring function according to the present invention;
FIG. 2 is a schematic structural diagram of a microfluidic chip of the cell culture device with temperature monitoring function according to the present invention;
FIG. 3 is a cell image collected when the temperature of the temperature-variable channel of the microfluidic chip is 34 ℃;
FIG. 4 is a peak value of RGB three-channel gray scale values of a cell image collected when the temperature of a temperature-varying channel is 34 ℃;
FIG. 5 is a cell image collected when the temperature of the temperature-variable channel of the microfluidic chip is 38 ℃;
FIG. 6 is a peak value of RGB three-channel gray scale values of cell images collected when the temperature of the temperature-varying channel is 38 ℃;
FIG. 7 is a cell image collected when the temperature of the temperature-variable channel of the microfluidic chip is 42 ℃;
FIG. 8 is a peak value of RGB three-channel gray scale values of cell images collected when the temperature of the temperature-varying channel is 42 ℃;
FIG. 9 is a cell image collected when the temperature of the temperature-variable channel of the microfluidic chip is 44 ℃;
FIG. 10 is the peak value of RGB three-channel gray scale values of cell images collected at a temperature-dependent channel temperature of 44 ℃.
In the figure, 1, a fixed support, 2, a light source support, 3, a convex lens support, 4, a microfluidic chip, 5, an image sensor, 6, a base, 7, a temperature change channel, 8, an inlet of the microfluidic channel, and 9, an outlet of the microfluidic channel.
Detailed Description
The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.
The cell culture device with the temperature monitoring function comprises a micro-fluidic chip 4, a light source and a lens bracket 3 provided with a convex lens, which are sequentially arranged from top to bottom, as shown in figure 1, wherein the light source and the convex lens are coaxial, the light source is fixed on a light source bracket 2, the micro-fluidic chip 4 is arranged on a base 6, a fixed bracket 1 is fixedly arranged on the base 6, and the light source bracket 2 and the lens bracket 3 are fixedly connected with the fixed bracket 1.
As shown in fig. 2, the microfluidic chip 4 is a three-fork microfluidic chip, the microfluidic chip 4 is provided with three microfluidic channel inlets 8 and one microfluidic channel outlet 9, one side of a main channel of the microfluidic chip 4 is provided with a temperature change channel 7, a temperature change material is filled in the temperature change channel 7, the temperature change material is temperature change ink, and an image sensor 5 is arranged on the temperature change channel 7 on the lower surface of the microfluidic chip 4.
The invention relates to a temperature monitoring and regulating method for cell culture, which is implemented by applying the cell culture device to culture cells according to the following steps:
step 1, fixing a micro-fluidic chip 4 and an image sensor 5, turning on a power supply, and connecting the image sensor 5 with a computer CPU (central processing unit) through a USB (universal serial bus) interface;
step 2, turning on a light source system, and adjusting the position of the light source to enable the light source, the convex lens and the image sensor to be on the same axis, so that imaging is clear;
step 4, the computer CPU firstly turns on the image sensor 5 for 25s-35s, the cultured cells and the cell culture solution are preheated through the heating of a chip in the image sensor 5, the image sensor 5 collects temperature change images of a temperature change channel at equal intervals after preheating, and the current cell culture temperature is judged by analyzing the gray value of RGB three channels of the temperature change images;
in the step 4, the gray values of RGB three channels of the temperature-varying cell image are analyzed to judge the current cell culture temperature, specifically, the temperature-varying images of the temperature-varying channels at different temperatures are collected, the gray value histograms of the RGB three channels of the temperature-varying image at different temperatures are counted, the range of the gray values of the RGB three channels corresponding to each temperature value is determined by the histogram, and the temperature value of the current cell culture is correspondingly determined according to the range of the gray values of the RGB three channels of the collected temperature-varying image.
And 5, correspondingly adjusting the culture temperature of the cells according to the temperature requirement of the cultured cells, and carrying out real-time monitoring and regulation of cell culture.
Step 5 specifically, the computer CPU compares the current cell culture temperature determined in step 4 with the standard temperature range of the cultured cells, and if the current cell culture temperature is lower than the minimum threshold value of the standard temperature range of the cultured cells, keeps the image sensor 5 powered on, and continuously raises the cell culture temperature by heating the chip in the image sensor 5 until the cell culture temperature falls within the standard temperature range of the cultured cells; if the current cell culture temperature is higher than the highest threshold value of the standard temperature range of the cultured cells, the flow rate of the cell culture solution is accelerated by the micropump so that the cell culture temperature is reduced until the cell culture temperature belongs to the standard temperature range of the cultured cells.
The principle of chip heating in the image sensor 5 is that a large number of transistors are integrated in the chip, the transistors heat current from the inside of the transistors, when the current passes through the transistors, conversion of electric energy can occur, and in the process of conduction, energy loss in the form of heat energy can be generated by the electric energy. That is, the transistor is restricted by on-state power consumption, switching power consumption and durability in the use process, and the operating temperature is increased due to power loss, so that the chip generates heat.
Example 1
The embodiment provides a temperature monitoring and regulating method for yeast cell culture, which is implemented by applying the cell culture device of the invention to culture cells according to the following steps:
step 1, fixing a micro-fluidic chip 4 and an image sensor 5, turning on a power supply, and connecting the image sensor 5 with a computer through a USB interface;
step 2, turning on a light source system, and adjusting the position of the light source to enable the light source, the convex lens and the image sensor 5 to be on the same axis, so that imaging is clear;
step 4, the computer CPU firstly turns on the image sensor 5 for 30s, preheats the cultured cells and the cell culture solution through the heating of the chip in the image sensor 5, the preheated image sensor 5 collects the yeast cell temperature change images of the temperature change channel 7 every 30s, as shown in FIG. 3, the yeast cell images collected at the temperature of 34 ℃ are shown, as shown in FIG. 4, the gray value peak values of RGB three channels corresponding to the collected temperature change images in FIG. 3 are respectively 0, 49 and 2, as shown in FIG. 5, the yeast cell images collected at the temperature of 38 ℃ are shown, as shown in FIG. 6, the gray value peak values of RGB three channels corresponding to the collected temperature change images in FIG. 5 are respectively 0, 75 and 5, as shown in FIG. 7, the yeast cell images collected at the temperature of 42 ℃, as shown in FIG. 8, the gray value peak values of RGB three channels corresponding to the collected temperature change images in FIG. 7 are respectively 0, 75 and 5, 96. 7, as shown in fig. 9, the yeast cell image collected at the temperature of 44 ℃, as shown in fig. 10, the gray value peak values of three RGB channels according to the collected temperature-change image corresponding to fig. 9 are respectively 0, 112, 12, so as to collect the yeast cell temperature-change image of the temperature-change channel 7 at different temperatures, count the gray value histograms of the three RGB channels of the temperature-change image at different temperatures, and determine the range of the gray value of the three RGB channels corresponding to each temperature value according to the histogram;
correspondingly determining the temperature value of the current cell culture according to the range of the gray values of the RGB three channels of the collected yeast cell temperature change image;
step 5, comparing the temperature value of the current cell culture judged in the step 4 with the standard temperature range of the cultured yeast cells by a computer CPU, if the temperature value of the current cell culture is lower than the lowest threshold value of the standard temperature range of the cultured yeast cells, keeping the image sensor 5 powered on, and continuously increasing the cell culture temperature through the heating of a chip in the image sensor 5 until the temperature value of the cell culture belongs to the standard temperature range of the cultured yeast cells; if the temperature value of the current cell culture is higher than the maximum threshold value of the standard temperature range of the cultured yeast cells, the flow rate of the cell culture solution is accelerated by the micropump so that the cell culture temperature is reduced until the temperature value of the cell culture belongs to the standard temperature range of the cultured yeast cells, and the cell culture is monitored and regulated in real time.
Example 2
The embodiment provides a temperature monitoring and regulating method for yeast cell culture, which is implemented by applying the cell culture device of the invention to culture cells according to the following steps:
step 1, fixing the microfluidic chip 4 and the image sensor 5, turning on a power supply, and connecting the image sensor with a computer CPU through a USB interface.
And 2, turning on a light source system, and adjusting the position of the light source to enable the light source, the convex lens and the image sensor to be on the same axis, so that imaging is clear.
And 3, introducing yeast cells from a microfluidic channel inlet 8 in the middle of the microfluidic chip 4, and introducing cell culture solution from microfluidic channel inlets 8 on two sides through a micropump.
Step 4, the computer CPU firstly turns on the image sensor 5 for 25s, the yeast cells and the cell culture solution are preheated by the chip heating in the image sensor 5, the image sensor 5 collects temperature change images of a temperature change channel every 25s after preheating, and the current cell culture temperature is judged by analyzing the gray value of RGB three channels of the temperature change images;
in the step 4, the gray values of RGB three channels of the temperature-varying cell image are analyzed to judge the current cell culture temperature, specifically, the temperature-varying images of the temperature-varying channels at different temperatures are collected, the gray value histograms of the RGB three channels of the temperature-varying image at different temperatures are counted, the range of the gray values of the RGB three channels corresponding to each temperature value is determined by the histogram, and the temperature value of the current cell culture is correspondingly determined according to the range of the gray values of the RGB three channels of the collected temperature-varying image.
Step 5, comparing the current cell culture temperature judged in the step 4 with the standard temperature range of the cultured yeast cells by a computer CPU, if the current cell culture temperature is lower than the lowest threshold value of the standard temperature range of the cultured yeast cells, keeping the image sensor 5 powered on, and continuously increasing the cell culture temperature through the heating of a chip in the image sensor 5 until the cell culture temperature belongs to the standard temperature range of the cultured yeast cells; if the current cell culture temperature is higher than the highest threshold value of the standard temperature range of the cultured yeast cells, the flow rate of the cell culture solution is accelerated by the micropump so that the cell culture temperature is reduced until the cell culture temperature belongs to the standard temperature range of the cultured yeast cells, and the cell culture is monitored and regulated in real time.
Example 3
The embodiment provides a temperature monitoring and regulating method for yeast cell culture, which is implemented by applying the cell culture device of the invention to culture cells according to the following steps:
step 1, fixing the microfluidic chip 4 and the image sensor 5, turning on a power supply, and connecting the image sensor 5 with a computer CPU through a USB interface.
And 2, turning on a light source system, and adjusting the position of the light source to enable the light source, the convex lens and the image sensor 5 to be on the same axis, so that imaging is clear.
And 3, introducing yeast cells from a microfluidic channel inlet 8 in the middle of the microfluidic chip 4, and introducing cell culture solution from microfluidic channel inlets 8 on two sides through a micropump.
Step 4, the computer CPU firstly turns on the image sensor 5 for 35s, the yeast cells and the cell culture solution are preheated by the chip heating in the image sensor 5, the image sensor 5 collects temperature change images of a temperature change channel every 35s after preheating, and the current cell culture temperature is judged by analyzing the gray value of RGB three channels of the temperature change images;
in the step 4, the gray values of RGB three channels of the temperature-varying cell image are analyzed to judge the current cell culture temperature, specifically, the temperature-varying images of the temperature-varying channels at different temperatures are collected, the gray value histograms of the RGB three channels of the temperature-varying image at different temperatures are counted, the range of the gray values of the RGB three channels corresponding to each temperature value is determined by the histogram, and the temperature value of the current cell culture is correspondingly determined according to the range of the gray values of the RGB three channels of the collected temperature-varying image.
Step 5, comparing the current cell culture temperature judged in the step 4 with the standard temperature range of the cultured yeast cells by a computer CPU, if the current cell culture temperature is lower than the lowest threshold value of the standard temperature range of the cultured yeast cells, keeping the image sensor 5 powered on, and continuously increasing the cell culture temperature through the heating of a chip in the image sensor 5 until the cell culture temperature belongs to the standard temperature range of the cultured yeast cells; if the current cell culture temperature is higher than the highest threshold value of the standard temperature range of the cultured yeast cells, the flow rate of the cell culture solution is accelerated by the micropump so that the cell culture temperature is reduced until the cell culture temperature belongs to the standard temperature range of the cultured yeast cells, and the cell culture is monitored and regulated in real time.
Example 4
The embodiment provides a temperature monitoring and regulating method for algae cell culture, which is implemented by applying the cell culture device to culture cells according to the following steps:
step 1, fixing the microfluidic chip 4 and the image sensor 5, turning on a power supply, and connecting the image sensor with a computer CPU through a USB interface.
And 2, turning on a light source system, and adjusting the position of the light source to enable the light source, the convex lens and the image sensor to be on the same axis, so that imaging is clear.
And 3, introducing algae cells from a microfluidic channel inlet 8 in the middle of the microfluidic chip 4, and introducing cell culture solution from microfluidic channel inlets 8 on two sides through a micropump.
Step 4, the computer CPU firstly turns on the image sensor 5 for electrifying for 32s, preheats the algae cells and the cell culture solution through the heating of a chip in the image sensor 5, collects temperature change images of a temperature change channel every 28s after preheating, and judges the current cell culture temperature by analyzing the gray value of RGB three channels of the temperature change images;
in the step 4, the gray values of RGB three channels of the temperature-varying cell image are analyzed to judge the current cell culture temperature, specifically, the temperature-varying images of the temperature-varying channels at different temperatures are collected, the gray value histograms of the RGB three channels of the temperature-varying image at different temperatures are counted, the range of the gray values of the RGB three channels corresponding to each temperature value is determined by the histogram, and the temperature value of the current cell culture is correspondingly determined according to the range of the gray values of the RGB three channels of the collected temperature-varying image.
Step 5, comparing the current cell culture temperature judged in the step 4 with the standard temperature range of the cultured algae cells by a computer CPU, if the current cell culture temperature is lower than the lowest threshold value of the standard temperature range of the cultured algae cells, keeping the image sensor 5 powered on, and continuously increasing the cell culture temperature through the heating of a chip in the image sensor 5 until the cell culture temperature belongs to the standard temperature range of the cultured algae cells; if the current cell culture temperature is higher than the highest threshold value of the standard temperature range of the cultured algae cells, the flow speed of the cell culture solution is accelerated by the micropump so that the cell culture temperature is reduced until the cell culture temperature belongs to the standard temperature range of the cultured algae cells, and the cell culture is monitored and regulated in real time.
Example 5
The embodiment provides a temperature monitoring and regulating method for blood cell culture, which is implemented by applying the cell culture device of the invention to culture cells according to the following steps:
step 1, fixing the microfluidic chip 4 and the image sensor 5, turning on a power supply, and connecting the image sensor 5 with a computer CPU through a USB interface.
And 2, turning on a light source system, and adjusting the position of the light source to enable the light source, the convex lens and the image sensor 5 to be on the same axis, so that imaging is clear.
And 3, introducing blood cells from the microfluidic channel inlet 8 in the middle of the microfluidic chip 4, and introducing cell culture solution from the microfluidic channel inlets 8 on the two sides through a micropump.
Step 4, the computer CPU firstly turns on the image sensor 5 for 27s, preheats the blood cells and the cell culture solution through the heating of a chip in the image sensor 5, collects temperature change images of a temperature change channel every 31s after preheating, and judges the current cell culture temperature by analyzing the gray value of RGB three channels of the temperature change images;
in the step 4, the gray values of RGB three channels of the temperature-varying cell image are analyzed to judge the current cell culture temperature, specifically, the temperature-varying images of the temperature-varying channels at different temperatures are collected, the gray value histograms of the RGB three channels of the temperature-varying image at different temperatures are counted, the range of the gray values of the RGB three channels corresponding to each temperature value is determined by the histogram, and the temperature value of the current cell culture is correspondingly determined according to the range of the gray values of the RGB three channels of the collected temperature-varying image.
Step 5, comparing the current cell culture temperature judged in the step 4 with the standard temperature range of the cultured blood cells by a computer CPU, if the current cell culture temperature is lower than the lowest threshold value of the standard temperature range of the cultured blood cells, keeping the image sensor 5 powered on, and continuously increasing the cell culture temperature through the heating of a chip in the image sensor 5 until the cell culture temperature belongs to the standard temperature range of the cultured blood cells; if the current cell culture temperature is higher than the highest threshold value of the standard temperature range of the cultured blood cells, the flow rate of the cell culture solution is accelerated by the micropump so that the cell culture temperature is reduced until the cell culture temperature belongs to the standard temperature range of the cultured blood cells, and then the cell culture is monitored and regulated in real time.
Claims (7)
1. Cell culture device with temperature monitoring function, its characterized in that includes micro-fluidic chip (4), light source, lens support (3) that are provided with convex lens that from top to bottom set gradually, the light source is with the axle center with convex lens, main entrance one side of micro-fluidic chip (4) is provided with temperature change passageway (7), it has the temperature change material to fill in temperature change passageway (7), surface temperature change passageway (7) department is provided with image sensor (5) under micro-fluidic chip (4).
2. The cell culture device with the temperature monitoring function according to claim 1, wherein the microfluidic chip (4) is a three-fork microfluidic chip, and the microfluidic chip (4) is provided with three microfluidic channel inlets (8) and one microfluidic channel outlet (9).
3. The cell culture apparatus with temperature monitoring function according to claim 1, wherein the temperature change material is a temperature change ink.
4. The cell culture device with the temperature monitoring function according to claim 1, wherein the light source is fixed on a light source support (2), the microfluidic chip (4) is arranged on a base (6), a fixing support (1) is fixedly arranged on the base (6), and both the light source support (2) and the lens support (3) are fixedly connected with the fixing support (1).
5. The method for monitoring and controlling the temperature of cell culture is characterized in that the method uses the cell culture device as claimed in claim 2 to culture cells, and is implemented according to the following steps:
step 1, fixing a micro-fluidic chip (4) and an image sensor (5), turning on a power supply, and connecting the image sensor (5) with a computer CPU (central processing unit) through a USB (universal serial bus) interface;
step 2, turning on a light source system, and adjusting the position of the light source to enable the light source, the convex lens and the image sensor (5) to be on the same axis, so that imaging is clear;
step 3, introducing cultured cells from a microfluidic channel inlet (8) in the middle of the microfluidic chip (4), and introducing cell culture solution from microfluidic channel inlets (8) on two sides through a micro pump;
step 4, collecting temperature-change images of the temperature-change channel (7) at equal time intervals by the image sensor (5), and judging the current cell culture temperature by analyzing gray values of RGB (red, green and blue) three channels of the temperature-change images;
and 5, correspondingly adjusting the culture temperature of the cells according to the temperature requirement of the cultured cells, and carrying out real-time monitoring and regulation of cell culture.
6. The cell culture device with the temperature monitoring function according to claim 5, wherein the step 4 of determining the current cell culture temperature by analyzing the gray scale values of the RGB three channels of the temperature-varying cell image comprises the steps of collecting temperature-varying images of the RGB three channels of the temperature-varying channel (7) at different temperatures, counting gray scale value histograms of the RGB three channels of the temperature-varying image at different temperatures, determining the range of the gray scale values of the RGB three channels corresponding to each temperature value according to the histogram, and correspondingly determining the temperature value of the current cell culture according to the range of the gray scale values of the RGB three channels of the collected temperature-varying image.
7. The cell culture apparatus with temperature monitoring function according to claim 5, wherein the step 5 is specifically that the computer CPU compares the current cell culture temperature determined in the step 4 with the standard temperature range of the cultured cells, if the current cell culture temperature is lower than the lowest threshold value of the standard temperature range of the cultured cells, the computer CPU turns on the image sensor (5), and the cell culture temperature is raised by heating the chip in the image sensor (5) until the cell culture temperature belongs to the standard temperature range of the cultured cells; if the current cell culture temperature is higher than the highest threshold value of the standard temperature range of the cultured cells, the flow rate of the cell culture solution is accelerated by the micropump so that the cell culture temperature is reduced until the cell culture temperature belongs to the standard temperature range of the cultured cells.
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