CN111567678A - Antibiotic-free livestock intestinal health-care agent and preparation method thereof - Google Patents
Antibiotic-free livestock intestinal health-care agent and preparation method thereof Download PDFInfo
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- CN111567678A CN111567678A CN202010392300.4A CN202010392300A CN111567678A CN 111567678 A CN111567678 A CN 111567678A CN 202010392300 A CN202010392300 A CN 202010392300A CN 111567678 A CN111567678 A CN 111567678A
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Abstract
The invention aims to provide a preparation method of a non-antibiotic livestock intestinal health-care agent, wherein the raw materials such as a microecological preparation, an antibacterial peptide preparation, a mucous membrane promoting preparation, a veterinary drug preparation and the like are subjected to combined treatment by adopting technologies such as targeted drug loading, controlled release coating, enteric coating, mixing and the like to prepare the non-antibiotic livestock intestinal health-care agent, and on the basis of ensuring that the health-care functions of the raw materials are fully exerted, the release time sequence is controlled according to the health-care mechanism of an organism by utilizing mutual promotion and superposition of the functions of the raw materials, so that the overall health-care effect of the non-antibiotic livestock intestinal health-care agent is further improved.
Description
Technical Field
The invention relates to the technical field of livestock intestinal disease protection, in particular to an antibiotic-free livestock intestinal health-care agent and a preparation method thereof.
Background
Livestock intestinal diseases are one of the common diseases of large-scale farms, wherein intestinal diarrhea seriously affects the survival rate of livestock, thereby causing great economic loss. Related researches show that the intestinal tract plays an important role in the process of digesting and absorbing nutrients and is an important immune barrier of the body. Livestock in a state of damaged intestinal tracts or underdeveloped intestinal tracts are lack of passive immunity and are more easily attacked by harmful bacteria to generate intestinal tract diseases, so that the absorption and utilization rate of nutrients of an individual is reduced, the growth rate is slowed down, a vicious circle is formed, and the survival of the individual is seriously threatened.
At present, large-scale farms are still dependent on antibacterial drugs for the treatment of diarrheal individuals and for the daily epidemic prevention of the population. Due to the excessive dependence and use of antibacterial drugs, problems of residues, drug resistance, environmental threats, even 'antibiotic-associated diarrhea' and the like are generated in livestock groups. Along with the development of science and technology and the improvement of living standard, people have higher requirements on animal welfare and food safety, and animal health care and 'nonreactive' breeding become industrial trends.
Disclosure of Invention
The invention aims to provide a preparation method of a non-antibiotic livestock intestinal health-care agent, which adopts technologies of targeted drug loading, controlled release coating, enteric coating, mixing and the like to carry out combined treatment on 4 raw materials such as a microecological preparation, an antibacterial peptide preparation, a mucous membrane promoting preparation, a veterinary drug preparation and the like to prepare the non-antibiotic livestock intestinal health-care agent.
The invention aims to provide a preparation method of a nonreactive livestock intestinal health-care agent, which comprises the following steps:
s1, respectively preparing micro-ecological powder, antibacterial peptide powder, mucosa promoting powder and Chinese veterinary drug powder;
s2, mixing the micro-ecological powder and the antibacterial peptide powder prepared in the step S1 in a proper proportion, and loading the mixture by using a targeting carrier to prepare microsphere powder;
s3, performing controlled release coating on the mucosa promoting powder prepared in the step S1 to prepare coated powder;
s4, mixing the microparticle powder prepared in the step S2 and the coated powder prepared in the step S3 according to a proper proportion, and coating the obtained mixture for the second time to prepare enteric microparticle powder;
s5, mixing the enteric-coated particle powder prepared in the step S4 and the veterinary drug powder prepared in the step S1 in a proper proportion, and preparing different dosage forms by adopting proper auxiliary materials and processes.
The invention adopts micro-ecological powder, antibacterial peptide powder, mucosa promoting powder and Chinese veterinary medicine powder as raw materials. In the raw materials, the microecological preparation can improve the health condition of the intestinal tract by adjusting the microecological balance of the intestinal tract of animals; the antibacterial peptide is induced and generated in the animal body, has higher selective immune activation and regulation functions without side effect compared with the antibiotic; the mucosa promoter has effects of promoting intestinal mucosa development and promoting nutrient absorption; the traditional Chinese veterinary medicine preparation applies the traditional Chinese medicine theory to animals for adjustment and treatment, has better delaying and controlling effects in the early stage of disease onset, and has good maintenance effect on normal intestinal environment. The preparation which has no resistance, high efficiency and stable health care function to the intestinal tracts of the livestock is prepared by combining the four raw materials.
The mechanism of the four raw materials for health care of the intestinal tract of the livestock is different, so that the invention utilizes the coating technology to carry out controlled release treatment on the different raw materials according to the action mechanism of the raw materials so as to ensure the full play of the efficacy of each component and the synergistic effect among the components.
The preparation process of the invention mainly comprises 1 time of medicine carrying, 2 times of coating and 3 times of mixing (as shown in figure 1):
mixing for the first time: mixing the microecological agent with the antibacterial peptide component;
carrying medicine for one time: loading the mixture obtained by the first mixing with a targeting vector;
third coating: coating the mucosa promoting powder for controlled release;
fourthly, mixing for the second time: mixing the powder obtained by carrying the medicine for the first time with the powder obtained by coating for the first time;
coating for the second time: enteric coating the mixture obtained by the second mixing;
sixthly, mixing for the third time: mixing the powder obtained by the second coating with the veterinary drug powder;
and (3) processing the mixture obtained by mixing for the third time as a main material by matching with proper auxiliary materials and a production process to obtain the antibiotic-free livestock intestinal health-care agent with different formulations.
Wherein,
in the first mixing, the microecological agent and the antibacterial peptide have the functions of regulating the microecological environment or activating immunity and the like and can be used for treating intestinal tract diseases of livestock, the two components can be enriched at the focus of intestinal tracts of the livestock by adopting one-time medicine carrying of a targeting carrier, so that the focus is ensured to have higher preparation concentration, higher immunocompetent substances are induced at the focus by utilizing the antibacterial peptide to promote focus recovery, and meanwhile, the unbalanced microecological environment at the focus is regulated by utilizing the microecological preparation to avoid repeated attack of the focus. The matching and combination of the antibacterial peptide and the microecological agent are beneficial to accelerating the recovery speed of the focus;
the first coating delays the release of the mucosa promoter to other components which are not coated with the controlled release coating, and the reason is that the mucosa promoter has the function of protecting the intestinal mucosa by covering the intestinal mucosa in a large area and adsorbing and separating substances on the surface of the mucosa to form an isolation environment on the surface of the intestinal mucosa, so that the self-repair and regeneration of the mucosa at the position are promoted. The mucosa promoter has stronger adsorption performance, and is not beneficial to the full play of the health care functions of other components if the mucosa promoter is released with other components simultaneously;
the second mixing and coating treatment is that the first mixing and targeting drug-loaded microecological agent and antibacterial peptide component and the controlled-release coated mucous membrane promoter are protected from being digested by the stomach of livestock by enteric coating and can enter the intestinal tract for release;
the third mixing is to mix the three components with the enteric coating protection with the traditional Chinese veterinary medicine agent, thereby forming the main component of the intestinal health-care agent without the antibiotics. The traditional Chinese veterinary medicine preparation has multiple and complex active ingredients, has various diffusion and action ways in a body, does not depend on enrichment in intestinal tracts, does not need to be coated, and can be transmitted to the intestinal tracts from the oral cavity, the pharynx, the esophagus, the stomach and the intestinal tracts so as to ensure the full play of the drug effect, simplify the production process and reduce the preparation cost.
In conclusion, the invention realizes the mutual promotion and cooperative matching of 4 health-care functions of the microecologics, the antibacterial peptides, the mucosa promoter, the Chinese veterinary medicament and the like by utilizing the technologies of targeted medicament loading, mixing, coating and the like, not only ensures the full play of the health-care effect of each component on the intestinal tracts of the livestock, but also improves the whole health-care effect of the health-care agent by the superposition of the functions of the components and the control of the release time sequence.
In the invention, the micro-ecological powder component comprises but is not limited to one or a mixture of more of lactic acid bacteria, bacillus and xylo-oligosaccharide;
the antibacterial peptide powder comprises one or more peptide structures;
the mucosa promoting powder component comprises one or more of sodium butyrate and glutamine;
the traditional Chinese veterinary medicine powder formula comprises but is not limited to Sijunzi decoction;
wherein, the micro-ecological powder and the antibacterial peptide powder adopt self-made or purchased freeze-dried powder; the veterinary Chinese medicine powder is self-made or purchased extract powder.
In the invention, the targeted medicine carrier of the microecological agent and the antibacterial peptide is prepared by the following steps:
s21, mixing the microecological powder and the antibacterial peptide powder according to a proper volume part ratio, and dissolving the mixture in water to prepare the bacterial strain with the concentration of not less than 1 х 106cFU/ml, and mixing with gelatin with the appropriate volume concentration of 5 mg/ml-8 mg/ml;
s22, mixing the components in a volume ratio of 0.1: 0.05: 1, preparing a targeting agent, a disintegrating agent and the mixed solution obtained in the step A1, mixing with span 80 oil with the appropriate volume concentration of 2 mg/ml-5 mg/ml, and uniformly mixing to obtain emulsion;
s23, mixing the emulsion obtained in the step A2 and a glutaraldehyde solution with the concentration of 10-20% in a proper proportion, standing at a low temperature for not less than 24 hours, and performing suction filtration to obtain a filter cake;
and S24, washing the filter cake obtained in the step A3 with ethanol and water in sequence until the filter cake is odorless, drying, and sorting and collecting microsphere powder with the target particle size by adopting a sample sorting sieve.
In the invention, the mucosa accelerant controlled-release powder is prepared by the following steps:
s31, mixing the mucosa promoting powder, the gelatin solution with the concentration of 5 mg/ml-8 mg/ml and the span 80 with the concentration of 2 mg/ml-5 mg/ml according to a proper proportion to prepare emulsion;
s32, mixing the emulsion obtained in the step S31 and a glutaraldehyde solution with the concentration of 10-20% in a proper proportion, standing at a low temperature for not less than 5 hours, and performing suction filtration to obtain a filter cake;
s33, washing the filter cake obtained in the step S32 with ethanol and water in sequence until the filter cake is odorless, drying, sorting by adopting a sample sorting sieve and collecting microspheres with target particle size;
s34, mixing the raw materials in parts by volume as 1: (10-15) preparing the microsphere powder prepared in the step S33 and a sustained-release material Eudragit L100-55, performing dry coating by taking liquid paraffin as a plasticizer, drying at 40 ℃, and sorting and collecting coating powder with a target particle size by adopting a sample separation sieve.
In the invention, the enteric coating powder is prepared by the following steps:
s41, mixing the micro-particle powder prepared in the step S2 and the coating powder prepared in the step S3 according to a proper proportion to prepare mixed micro-particle powder;
s42, mixing the raw materials in parts by volume as 1: (3-5) preparing the mixed particle powder prepared in the step S41 and an enteric coating material Eudragit L100, performing secondary dry coating, drying at 40 ℃, and sorting and collecting the enteric particle powder with the target particle size by adopting a sample separation sieve.
Based on the preparation method, the antibiotic-free livestock intestinal health-care granules can be prepared by the following steps:
s51, mixing the enteric-coated particle powder prepared in the step S42 and the traditional Chinese veterinary medicine powder prepared or purchased in the step S1 in a proper proportion to prepare mixed powder;
s52, mixing the mixed powder prepared in the step S51, fish oil and auxiliary materials in a proper proportion to prepare suspension;
s53, adopting a spray freezing granulation process to process the suspension prepared in the step S52 to prepare the livestock intestinal health-care granules without antibiotics.
4 raw materials of the microecological preparation, the antibacterial peptide preparation, the mucous membrane promoting preparation, the veterinary drug preparation and the like all have biological activity, so that the process temperature is strictly controlled when the microecological preparation, the antibacterial peptide preparation, the mucous membrane promoting preparation, the veterinary drug preparation and the like are processed, and a low-temperature forming process is selected as far as possible to process the dosage form of a final product.
The invention also aims to provide an antibiotic-free livestock intestinal health-care agent, which does not contain antibiotic and other antibacterial drugs, so that the health of livestock individuals cannot be influenced by long-time feeding, the problems of residue, drug resistance, environmental threat and the like cannot be caused in livestock groups, the animal welfare is improved, and antibiotic-free breeding is really realized.
The main material of the antibiotic-free livestock intestinal health agent is mixed powder formed by composite particle powder and traditional Chinese veterinary medicine powder. The structure of the composite micro-particle powder is shown in fig. 2, and the composite micro-particle powder has a coating 10, and a plurality of first micro-sphere particles 20 and a plurality of second micro-sphere particles 30 are distributed in the coating 10. The first microspheroidal particle 20 is loaded with a microecological preparation 1, an antimicrobial peptide preparation 2 and a small amount of a disintegrant 3, and has several specific groups 4 on the surface. The second microspheroidal particle 30 is loaded with a mucosa-promoting agent 5, the surface of which also has a coating shell 6.
Wherein,
the coating 10 is an enteric coating which can be stably kept in the stomach of livestock and can be dissolved in the intestinal tract, thereby protecting the granule powder inside the livestock from being digested by the stomach and enriched in the intestinal tract;
the specific group 4 on the surface of the first microsphere particle 20 has the function of targeting a focus of livestock intestinal inflammation, and the specific group 4 targets the focus in the livestock intestinal tract to enrich the first microsphere particle 20 at the focus, so that the utilization rate of the microecological preparation 1 and the antibacterial peptide preparation 2 loaded by the microsphere particles is improved, and the recovery speed of the focus is accelerated;
the first microsphere particles 20 also contain a small amount of disintegrant 3, and the disintegrant 3 is used for the quick release of the microecological preparation 1 and the antibacterial peptide preparation 2, and is beneficial to improving the action efficiency of the microecological preparation 1 and the antibacterial peptide preparation 2 on livestock intestinal tracts.
The coating body 6 of the second microsphere particle 30 is a controlled-release coating, which can be retained in the intestinal tract of livestock for a long time and can be kept stable for a long time, so that the mucosa promoter 5 loaded on the second microsphere particle 30 is delayed from other raw material components to be released into the intestinal tract.
The antibiotic-free livestock intestinal health-care agent is processed by different forming processes to obtain antibiotic-free livestock intestinal health-care agents in different dosage forms, wherein the dosage forms comprise medicinal dosage forms such as powder, granules, tablets and oral liquid, and also comprise food forms such as sugar, beverage and feed.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings used in the description of the present specification will be briefly described below.
FIG. 1 is a schematic diagram of the preparation process adopted in the present invention.
FIG. 2 is a schematic structural diagram of some components of the antibiotic-free livestock intestinal health agent prepared by the invention.
Reference numerals: 10. 20 parts of a coating shell, 20 parts of first microsphere particles, 30 parts of second microsphere particles, 1 part of a microecological preparation, 2 parts of an antibacterial peptide preparation, 3 parts of a disintegrating agent, 4 parts of a specificity group, 5 parts of a mucosa promoter and 6 parts of a coating shell.
Detailed Description
Only certain exemplary embodiments are briefly described below. As those skilled in the art will recognize, the described embodiments may be modified in various different ways, all without departing from the spirit or scope of the claimed embodiments. Accordingly, the description is to be regarded as illustrative in nature and not as restrictive.
Example 1
The embodiment provides a preparation method of antibiotic-free livestock intestinal health granules, which comprises the following steps:
1. respectively purchasing or preparing viable bacteria with number of not less than 108The lactic acid bacteria powder, the antibacterial peptide powder (which is induced by the same species as the target livestock, if the bred livestock is pigs, the swine antibacterial peptide PR-39 can be used), the sodium butyrate powder and the four-monarch liquid powder for livestock;
2. according to the volume part ratio (5-10): 1: (1-3) mixing and dispersing lactic acid bacteria powder and swine antibacterial peptide PR-39 powder with water to prepare the mixture with the thallus concentration of not less than 1 х 106cFU/ml suspension;
3. according to the volume part ratio of 10: (1-3) mixing the suspension obtained in the step (2) with a gelatin solution with the concentration of (5-8) mg/ml;
4. according to the volume part ratio of 1: 0.5: 10 adding immunomagnetic beads and polysorbate 80 into the mixed solution obtained in the step 3, uniformly mixing, and stirring and dropwise adding a proper amount of span 80 with the concentration of (2-5) mg/ml to prepare an emulsion;
5. according to the volume part ratio of 1: (1-3) mixing the emulsion obtained in the step (4) with a glutaraldehyde solution with the concentration of 10-20%, standing for 24 hours at the temperature of below 10 ℃, and filtering to obtain a filter cake;
6. washing the filter cake obtained in the step 5 with ethanol and water in sequence until the filter cake is odorless, drying and crushing, sorting the microsphere powder with the particle size of 60-80 mu m by using 180-mesh and 220-mesh sample separation sieves, and detecting that the drug loading of the microsphere powder is 25.3% and the encapsulation rate is 95.3% by adopting an HPLC method, so as to meet the requirement for later use;
7. according to the volume part ratio (1-3): 10, mixing sodium butyrate powder with gelatin solution with the concentration of (5-8) mg/ml, uniformly mixing, stirring and dropwise adding a proper amount of span 80 with the concentration of (2-5) mg/ml to prepare emulsion;
8. according to the volume part ratio of 1: (1-3) mixing the emulsion obtained in the step (7) with a glutaraldehyde solution with the concentration of 10-20%, standing for 5 hours at the temperature of below 10 ℃, and filtering to obtain a filter cake;
9. washing the filter cake obtained in the step 8 with ethanol and water in sequence until the filter cake is odorless, drying and crushing, sorting microsphere powder with the particle size of 65-75 micrometers by using 200-mesh and 250-mesh sample sieves, and detecting that the drug-loading rate of the microsphere powder is 17.0% and the encapsulation rate is 97.4% by adopting an HPLC method, so as to meet the requirement for later use;
10. according to the volume part ratio of 1: (1-2) mixing the two kinds of microsphere powder prepared in the step 6 and the step 9, and then mixing the two kinds of microsphere powder according to the volume part ratio of 1: (3-5) mixing the two mixed microsphere powder bodies with an enteric coating material Eudragit L100, performing dry coating by using liquid paraffin as a plasticizer, and drying at 40 ℃;
11. powder particles with the particle size of 250-380 mu m are separated and collected by using a 40-mesh sample separation sieve and a 60-mesh sample separation sieve, the drug-loading rate of the particle powder is 52.7 percent and the encapsulation rate is 96.1 percent by adopting an HPLC method, and the particle powder meets the requirement for later use;
12. the weight portion ratio of 2: 2: 2: 3: 3: 3: 3: 3: 4: 2.5 preparing radix codonopsitis, radix astragali, hawthorn, rhizoma atractylodis macrocephalae, poria cocos, rhizoma alismatis, medicated leaven, malt, fructus schizandrae, Chinese yam, akebia stem and liquorice (the taste of the four-monarch drug decoction is added), decocting for 30min to 45min, taking decoction, concentrating the decoction to prepare extract, drying the extract until the water content is less than 4%, crushing the extract, preparing powder, and sieving the powder with a 60-mesh sieve to obtain the traditional Chinese veterinary drug powder;
13. according to the volume part ratio (1-5): 1, mixing the particle powder prepared in the step 11 with the veterinary Chinese medicine powder prepared in the step 12 to obtain a raw material of the antibiotic-free livestock intestinal health-care granule;
14. according to the mass part ratio (10-100): 10: 1, mixing fish oil and forming auxiliary materials into the raw materials prepared in the step 13 to prepare viscous suspension;
15. and (3) freezing and granulating the suspension prepared in the step (14) by adopting a spray freezing and granulating process to obtain antibiotic-free livestock intestinal health-care granules, subpackaging and storing at the temperature of below 20 ℃.
The antibiotic-free livestock intestinal health-care agent prepared by the embodiment is a granular preparation, and when the granular preparation is used for daily health care, the granular preparation can be dissolved in drinking water of livestock for direct drinking, and also can be used as an additive to be added into daily feed.
Example 2
The embodiment provides a preparation method of antibiotic-free livestock intestinal tract health-care candy, which comprises the following steps:
1. respectively purchasing or preparing total viable bacteria of not less than 108The mixed powder of lactobacillus and bacillus, antibacterial peptide (different species can be adopted, such as cecropin-B type antibacterial peptide), glutamine powder and veterinary Sijunzi decoction powder;
2. according to the volume part ratio (5-10): 1: (1-3) mixing and dispersing lactic acid bacteria powder and cecropin-B type antibacterial peptide powder with water to prepare a mixture with the thallus concentration of not less than 1 х 106cFU/ml suspension;
3. according to the volume part ratio of 10: (1-3) mixing the suspension obtained in the step (2) with a gelatin solution with the concentration of (5-8) mg/ml;
4. according to the volume part ratio of 1: 0.5: 10 adding immunomagnetic beads and polysorbate 80 into the mixed solution obtained in the step 3, uniformly mixing, and stirring and dropwise adding a proper amount of span 80 with the concentration of (2-5) mg/ml to prepare an emulsion;
5. according to the volume part ratio of 1: (1-3) mixing the emulsion obtained in the step (4) with a glutaraldehyde solution with the concentration of 10-20%, standing for 24 hours at the temperature of below 10 ℃, and filtering to obtain a filter cake;
6. washing the filter cake obtained in the step 5 with ethanol and water in sequence until the filter cake is odorless, drying and crushing, sorting the microsphere powder with the particle size of 60-80 mu m by using 180-mesh and 220-mesh sample separation sieves, and detecting that the drug loading of the microsphere powder is 30.9% and the encapsulation rate is 95.8% by adopting an HPLC method, so as to meet the requirement for later use;
7. according to the volume part ratio (1-3): 10, mixing glutamine powder and gelatin solution with the concentration of (5-8) mg/ml, uniformly mixing, stirring and dropwise adding a proper amount of span 80 with the concentration of (2-5) mg/ml to prepare emulsion;
8. according to the volume part ratio of 1: (1-3) mixing the emulsion obtained in the step (7) with a glutaraldehyde solution with the concentration of 10-20%, standing for 5 hours at the temperature of below 10 ℃, and filtering to obtain a filter cake;
9. washing the filter cake obtained in the step 8 with ethanol and water in sequence until the filter cake is odorless, drying and crushing, sorting microsphere powder with the particle size of 65-75 micrometers by using 200-mesh and 250-mesh sample sieves, and detecting that the drug-loading rate of the microsphere powder is 17.0% and the encapsulation rate is 97.4% by adopting an HPLC method, so as to meet the requirement for later use;
10. according to the volume part ratio of 1: (1-2) mixing the two kinds of microsphere powder prepared in the step 6 and the step 9, and then mixing the two kinds of microsphere powder according to the volume part ratio of 1: (3-5) mixing the two mixed microsphere powder bodies with an enteric coating material Eudragit L100, performing dry coating by using liquid paraffin as a plasticizer, and drying at 40 ℃;
11. powder particles with the particle size of 250-380 mu m are separated and collected by using a 40-mesh sample separation sieve and a 60-mesh sample separation sieve, the drug-loading rate of the particle powder is 52.7 percent and the encapsulation rate is 96.1 percent by adopting an HPLC method, and the particle powder meets the requirement for later use;
12. the weight portion ratio of 2: 2: 2: 3: 3: 3: 3: 3: 4: 2.5 preparing radix codonopsitis, radix astragali, hawthorn, rhizoma atractylodis macrocephalae, poria cocos, rhizoma alismatis, medicated leaven, malt, fructus schizandrae, Chinese yam, akebia stem and liquorice (the taste of the four-monarch drug decoction is added), decocting for 30min to 45min, taking decoction, concentrating the decoction to prepare extract, drying the extract until the water content is less than 4%, crushing the extract, preparing powder, and sieving the powder with a 60-mesh sieve to obtain the traditional Chinese veterinary drug powder;
13. according to the volume part ratio (1-5): 1, mixing the particle powder prepared in the step 11 with the veterinary drug powder prepared in the step 12 to obtain a main material of the antibiotic-free livestock intestinal health candy;
14. according to the mass part ratio of 1: (1-15) mixing refined sugar powder and gelatin as a necessary amount into the main material prepared in the step 13 to serve as an adhesive, performing tabletting molding on the raw materials by adopting a cold processing technology to prepare tabletted candies, and storing the tabletted candies below 20 ℃ after packaging.
The livestock intestinal health-care agent without antibiotics prepared by the embodiment is in the form of candy, and candy products with different health-care effects can be prepared by adjusting the proportion of the main material and auxiliary materials such as refined powdered sugar, gelatin and the like in step 14, so that daily health care or disease nursing can be carried out. Because the sweet candy has high sweetness, the livestock such as pigs, horses and the like sensitive to the sweet taste can be more easily received and is convenient for feeding.
Claims (10)
1. The preparation method of the antibiotic-free livestock intestinal health-care agent is characterized by comprising the following steps:
s1, respectively preparing micro-ecological powder, antibacterial peptide powder, mucosa promoting powder and Chinese veterinary drug powder;
s2, mixing the micro-ecological powder and the antibacterial peptide powder prepared in the step S1 in a proper proportion, and loading the mixture by using a targeting carrier to prepare microsphere powder;
s3, performing controlled release coating on the mucosa promoting powder prepared in the step S1 to prepare coated powder;
s4, mixing the microparticle powder prepared in the step S2 and the coated powder prepared in the step S3 according to a proper proportion, and coating the obtained mixture for the second time to prepare enteric microparticle powder;
s5, mixing the enteric-coated particle powder prepared in the step S4 and the veterinary drug powder prepared in the step S1 in a proper proportion, and preparing different dosage forms by adopting proper auxiliary materials and processes.
2. The method according to claim 1, wherein step S2 specifically includes:
s21, mixing the microecological powder and the antibacterial peptide powder according to a proper volume part ratio, and dissolving the mixture in water to prepare the bacterial strain with the concentration of not less than 1 х 106cFU/ml, and mixing with gelatin with the appropriate volume concentration of 5 mg/ml-8 mg/ml;
s22, mixing the components in a volume ratio of 0.1: 0.05: 1, preparing a targeting agent, a disintegrating agent and the mixed solution obtained in the step A1, mixing with span 80 oil with the appropriate volume concentration of 2 mg/ml-5 mg/ml, and uniformly mixing to obtain emulsion;
s23, mixing the emulsion obtained in the step A2 and a glutaraldehyde solution with the concentration of 10-20% in a proper proportion, standing at a low temperature for not less than 24 hours, and performing suction filtration to obtain a filter cake;
and S24, washing the filter cake obtained in the step A3 with ethanol and water in sequence until the filter cake is odorless, drying, and sorting and collecting microsphere powder with the target particle size by adopting a sample sorting sieve.
3. The preparation method according to claim 2, wherein the drug loading rate of the microspheres in the microsphere powder prepared in step S24 on the microecological component and the antibacterial peptide component is not less than 10% and the encapsulation rate is not less than 95%.
4. The method according to claim 1, wherein step S3 specifically includes:
s31, mixing the mucosa promoting powder, the gelatin solution with the concentration of 5 mg/ml-8 mg/ml and the span 80 with the concentration of 2 mg/ml-5 mg/ml according to a proper proportion to prepare emulsion;
s32, mixing the emulsion obtained in the step S31 and a glutaraldehyde solution with the concentration of 10-20% in a proper proportion, standing at a low temperature for not less than 5 hours, and performing suction filtration to obtain a filter cake;
s33, washing the filter cake obtained in the step S32 with ethanol and water in sequence until the filter cake is odorless, drying, sorting by adopting a sample sorting sieve and collecting microspheres with target particle size;
s34, mixing the raw materials in parts by volume as 1: (10-15) preparing the microsphere powder prepared in the step S33 and a sustained-release material Eudragit L100-55, performing dry coating by taking liquid paraffin as a plasticizer, drying at 40 ℃, and sorting and collecting coating powder with a target particle size by adopting a sample separation sieve.
5. The preparation method of claim 4, wherein the drug loading rate of the microspheres prepared in step S33 is not less than 10% and the encapsulation efficiency is not less than 95%.
6. The method according to claim 1, wherein step S4 specifically includes:
s41, mixing the micro-particle powder prepared in the step S2 and the coating powder prepared in the step S3 according to a proper proportion to prepare mixed micro-particle powder;
s42, mixing the raw materials in parts by volume as 1: (3-5) preparing the mixed particle powder prepared in the step S41 and an enteric coating material Eudragit L100, performing secondary dry coating, drying at 40 ℃, and sorting and collecting the enteric particle powder with the target particle size by adopting a sample separation sieve.
7. The method according to claim 1, wherein step S5 specifically includes:
s51, mixing the enteric-coated particle powder prepared in the step S4 and the veterinary drug powder prepared in the step S1 in a proper proportion to prepare mixed powder;
s52, mixing the mixed powder prepared in the step S51, fish oil and auxiliary materials in a proper proportion to prepare suspension;
s53, adopting a spray freezing granulation process to process the suspension prepared in the step S52 to prepare the livestock intestinal health-care granules without antibiotics.
8. The production method according to any one of claims 1 to 7,
the micro-ecological powder component comprises one or more of but not limited to lactic acid bacteria, bacillus and xylo-oligosaccharide;
the antibacterial peptide powder comprises one or more peptide structures;
the mucosa promoting powder component comprises one or more of sodium butyrate and glutamine;
the traditional Chinese veterinary medicine powder formula comprises but is not limited to Sijunzi decoction;
wherein,
the micro-ecological powder and the antibacterial peptide powder are self-made or purchased freeze-dried powder;
the veterinary Chinese medicine powder is self-made or purchased extract powder.
9. An antibiotic-free livestock intestinal health agent characterized by being prepared by the preparation method according to any one of claims 1 to 8.
10. The health agent as claimed in claim 9, wherein the form of the health agent includes pharmaceutical dosage forms and food forms.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554328A (en) * | 2003-12-25 | 2004-12-15 | 浙江大学 | Microball preparation for intestine orient releasing medicne and its preparing method |
| US20140056980A1 (en) * | 2012-04-30 | 2014-02-27 | Tillotts Pharma Ag | Delayed release drug formulation |
| CN104546907A (en) * | 2014-12-24 | 2015-04-29 | 浙江惠嘉生物科技有限公司 | Enteric nano zinc oxide preparation and preparation method thereof |
| CN105410338A (en) * | 2015-12-17 | 2016-03-23 | 百洋产业投资集团股份有限公司 | Antibiotic-free daily fodder for piglets and preparation method of daily fodder |
| CN105963688A (en) * | 2016-06-23 | 2016-09-28 | 四川英格瑞生物科技有限公司 | Powder for curing intestinal diseases of piglet and enhancing immunity and preparation method thereof |
| CN109984276A (en) * | 2019-04-26 | 2019-07-09 | 山东祥维斯生物科技股份有限公司 | A kind of nonreactive feed addictive and preparation method thereof improving enteron aisle stress reaction |
| CN110897048A (en) * | 2019-12-30 | 2020-03-24 | 广州格雷特生物科技有限公司 | Enteric sodium butyrate microcapsule and preparation method and application thereof |
| CN110923170A (en) * | 2019-12-18 | 2020-03-27 | 福建大用投资有限公司 | Composite microecological preparation for three-dimensional epidemic prevention of nonreactive breeding of live pigs and preparation method and application thereof |
-
2020
- 2020-05-11 CN CN202010392300.4A patent/CN111567678A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1554328A (en) * | 2003-12-25 | 2004-12-15 | 浙江大学 | Microball preparation for intestine orient releasing medicne and its preparing method |
| US20140056980A1 (en) * | 2012-04-30 | 2014-02-27 | Tillotts Pharma Ag | Delayed release drug formulation |
| CN104546907A (en) * | 2014-12-24 | 2015-04-29 | 浙江惠嘉生物科技有限公司 | Enteric nano zinc oxide preparation and preparation method thereof |
| CN105410338A (en) * | 2015-12-17 | 2016-03-23 | 百洋产业投资集团股份有限公司 | Antibiotic-free daily fodder for piglets and preparation method of daily fodder |
| CN105963688A (en) * | 2016-06-23 | 2016-09-28 | 四川英格瑞生物科技有限公司 | Powder for curing intestinal diseases of piglet and enhancing immunity and preparation method thereof |
| CN109984276A (en) * | 2019-04-26 | 2019-07-09 | 山东祥维斯生物科技股份有限公司 | A kind of nonreactive feed addictive and preparation method thereof improving enteron aisle stress reaction |
| CN110923170A (en) * | 2019-12-18 | 2020-03-27 | 福建大用投资有限公司 | Composite microecological preparation for three-dimensional epidemic prevention of nonreactive breeding of live pigs and preparation method and application thereof |
| CN110897048A (en) * | 2019-12-30 | 2020-03-24 | 广州格雷特生物科技有限公司 | Enteric sodium butyrate microcapsule and preparation method and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 梁建友;王元有;: "金雀异黄素靶向明胶微球的制备与表征" * |
| 苏诗娜;吕竹芬;梁超峰;卢克伟;黄韵然;陈燕忠;: "新辅料和新技术在结肠定位制剂中的应用" * |
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Application publication date: 20200825 |