CN111544481A

CN111544481A - Herbal antibacterial liquid

Info

Publication number: CN111544481A
Application number: CN202010603708.1A
Authority: CN
Inventors: 谢汝石
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-29
Filing date: 2020-06-29
Publication date: 2020-08-18

Abstract

The invention discloses herbal bacteriostatic liquid which comprises the following raw materials in parts by mass: 3-8 g of lonicera confusa, 3-8 g of radix scutellariae, 3-8 g of oldenlandia diffusa, 3-8 g of big-mouth bowl and over 8 mu g/mL of pyrroloquinoline quinone liquid. The formula is a herbal formula, has no irritation and toxicity, does not contain hormone and antibiotic, does not have the side effect of the hormone, does not have the problems of cross sensitization and cross drug resistance with other antibiotics, has the advantages of good treatment effect, no relapse after healing, and has better bacteriostatic rate effect on escherichia coli, staphylococcus aureus and candida.

Description

Herbal antibacterial liquid

Technical Field

The invention relates to a bacteriostatic liquid for treating skin diseases, in particular to a herbal bacteriostatic liquid.

Background

The existing skin diseases, including whelk, dermatophyte infection (tinea), dermatitis, eczema and the like, are common frequently encountered diseases and are difficult to cure, and particularly eczema and the like not only affect the beauty of skin, but also are extremely itchy and difficult to accept. Most of the medicines on the market at present are antibiotics and hormone western medicines, and the treatment effect is unsatisfactory.

Disclosure of Invention

The invention aims to provide herbal bacteriostatic liquid to solve the problem that most of the currently popular medicines in the market are antibiotics and hormone western medicines, and the treatment effect is unsatisfactory in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme: the herbal antibacterial liquid comprises the following raw materials in parts by mass: 3-8 g of lonicera confusa, 3-8 g of radix scutellariae, 3-8 g of oldenlandia diffusa, 3-8 g of big-mouth bowl and over 8 mu g/mL of pyrroloquinoline quinone liquid.

Further preferably, the herbal bacteriostatic liquid comprises the following raw materials by mass: 5g of lonicera confusa, 5g of radix scutellariae, 5g of oldenlandia diffusa, 5g of big-mouth bowl and over 8 mu g/mL of pyrroloquinoline quinone liquid.

The preparation method of the herbal antibacterial liquid comprises the following steps: carefully selecting lonicera confusa, scutellaria baicalensis, oldenlandia diffusa and Chinese mugwort according to the mass raw material ratio of the herbal antibacterial liquid, drying, crushing into 80-100 mesh powder, extracting the liquid medicine components by using an extraction technology, adding pyrroloquinoline quinone liquid, and finally adding sterilized mineral water to prepare the antibacterial liquid.

Compared with the prior art, the invention has the beneficial effects that: the formula is a herbal formula, has no irritation and toxicity, does not contain hormone and antibiotic, does not have the side effect of the hormone, does not have the problems of cross sensitization and cross drug resistance with other antibiotics, has the advantages of good treatment effect, no relapse after healing, and has better bacteriostatic rate effect on escherichia coli, staphylococcus aureus and candida.

Drawings

FIG. 1 is a diagram of the skin condition of the affected area of a patient of this particular case on a first day of treatment;

FIG. 2 is a diagram of the skin condition of the affected part of the patient on the fourth day of treatment in this embodiment;

fig. 3 is a diagram of a seventh skin condition for the treatment of an affected area of a patient in this particular case.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1: the preparation method of the herbal antibacterial liquid comprises the following steps: according to the mass raw material ratio of the herbal antibacterial liquid, 3g of lonicera confusa, 3g of radix scutellariae, 3g of oldenlandia diffusa and 3g of big-mouth bowl are carefully selected, dried and crushed into 80-100-mesh powder, then the liquid medicine components are extracted by an extraction technology, then pyrroloquinoline quinone liquid is added to 8 mu g/mL, and finally the pyrroloquinoline quinone liquid is added to sterilized mineral water to prepare the antibacterial liquid.

Example 2: the preparation method of the herbal antibacterial liquid comprises the following steps: according to the mass raw material ratio of the herbal antibacterial liquid, 5g of lonicera confusa, 5g of radix scutellariae, 5g of oldenlandia diffusa and 5g of big-mouth bowl are carefully selected, dried and crushed into 80-100-mesh powder, then the liquid medicine components are extracted by an extraction technology, then pyrroloquinoline quinone liquid is added to 8 mu g/mL, and finally the pyrroloquinoline quinone liquid is added to sterilized mineral water to prepare the antibacterial liquid.

Example 3: the preparation method of the herbal antibacterial liquid comprises the following steps: according to the mass raw material ratio of the herbal antibacterial liquid, 8g of lonicera confusa, 8g of radix scutellariae, 8g of oldenlandia diffusa and 8g of big-mouth bowl are carefully selected, dried and crushed into 80-100-mesh powder, then the liquid medicine components are extracted by an extraction technology, then 8 mu g/mL of pyrroloquinoline quinone liquid is added, and finally the pyrroloquinoline quinone liquid is added into sterilized mineral water to be prepared.

Firstly, the herbal bacteriostatic solution prepared in the example 2 is subjected to bacteriostatic performance detection.

1. Test Strain Escherichia coli (ATCC 25922)

Staphylococcus aureus (ATCC6538)

The generation number of the strain above Candida albicans (ATCC10231) is the fifth generation.

2. Test method

2-1, preparing bacterial suspension, namely selecting the bacterial content according to a test method of a bacteriostatic test of a GB15979-2002 appendix C productIs 1 × 10⁴cfu/mLˉ 9× 10⁴cfu/mL of bacterial suspension for testing.

And 2-2, carrying out an antibacterial performance test according to appendix C of GB15979-2002, wherein the sample acts for 20 minutes, and the test is repeated for 3 times.

2-3, testing conditions are that the temperature is 25.8 ℃ and the humidity is 57%.

The results are shown in Table 1

TABLE 1

As can be seen from Table 1, the bacteriostatic agent prepared in this example 2 has a bacteriostatic rate of 99.99% against Escherichia coli, Staphylococcus aureus and Candida.

Secondly, the herbal bacteriostat prepared in the example 2 is subjected to a plurality of skin irritation tests.

Test animals: rabbit

The test method comprises the following steps: l, animal treatment, namely removing the rabbit hair at two sides of the spine of the back of the rabbit 24 hours before the test, wherein the hair removing range is about 3cm multiplied by 3cm respectively at the left side and the right side.

2. Contamination with toxin 0.5mL of the herbal bacteriostatic solution prepared in example 2 was applied directly to the skin of the hair-removed area, and the other side was used as a control and applied continuously for a day. Starting on the following day, the test and control areas were shaved 1h before each application and the remaining test article was removed with water. And (4) observing results after 1h, and judging the skin irritation intensity according to skin irritation intensity grading standards.

Table 2 below shows the test results

TABLE 2

It can be seen from table 2 that the herbal bacteriostatic agent prepared in this example 2 is non-irritating.

Thirdly, the herbal bacteriostat prepared in example 2 is subjected to an acute eye irritation test.

Test animals: rabbit

Negative control: physiological saline;

and (3) testing the sample: the herbal bacteriostatic agent prepared in the embodiment 2 is directly dropped;

the test method comprises the following steps: 3 New Zealand rabbits were examined 24h before the experiment for abnormalities in both eyes of the test animals by means of an auxiliary light source and further examined for fluorescein. If there is no abnormality, the lower eyelid of one side of the eye of the rabbit is opened, 0.1mL (100mg) of the test substance is dropped (or applied) into the conjunctival sac, so that the upper and lower eyelids are passively closed for 1s, and the other side of the eye is not treated for self-control. After the test substance is dripped, eyes are not washed within h. If the test substance is irritating or a product to be washed after use, the 3Os is washed with a sufficient amount of water having a high flow rate and causing no damage to the eyes of the animal 30s after the test substance is dropped. The eyes of the animals are checked at 1h, 24h, 48h, 72 h, 4d and 7d after the dropping of the test substances, and the observation time can be prolonged to 21d when the eyes of the animals do not recover within 7 d.

The test results are shown in Table 3

TABLE 3

It can be seen from table 3 that the herbal bacteriostatic agent prepared in this example 2 is non-irritating.

Fourthly, the herbal bacteriostat prepared in example 2 is subjected to a skin allergy test (topical occlusive skin test)

Test animals: albino guinea pig

Sample preparation: negative control, normal saline, coated on sterile patch.

The test sample, the herbal bacteriostat prepared in example 2, is directly coated on a sterile application.

The test method comprises the following steps: the number of common albino guinea pigs is 30, and the two groups are divided into 20 test groups and 10 negative control groups. The hair of the animal test site was removed 2h before the test.

1. Induction of contact by selecting the appropriate test sample concentration, soaking the appropriate patch with approximately 0.2mL (g) of the test substance, topically applying to the hair removal area of each animal, fixing for 6h with a non-irritating adhesive tape and patch, repeating this procedure at 7d and 14d, and operating with the same procedure as for the control animal using only blank liquid.

2. The priming stage, 14d-28d after the last induction application, the test sample was soaked in about 0.2mL (g) of the patch and applied to the unhaired untested site of each animal, and the whole test animal was primed by fixing with a non-irritating adhesive tape and patch for 6h, using the blank leaching medium as a control animal. After challenge contact skin reactions were observed at 24h and 48h and scored.

The specific test results are shown in Table 4

TABLE 4

As shown in Table 4, the bacteriostatic agent prepared in this example 2 showed no skin allergy.

Fifthly, acute percutaneous toxicity test is carried out on the herbal bacteriostatic solution prepared in the example 2

Test animals: guinea pig

Sample preparation: negative control, normal saline, coated on sterile patch.

The test method comprises the following steps: the maximum limit method is adopted, namely, 2000mg/kgg bodies are smeared on the skin of 10 animals

And (4) heavy dosage. Selecting a proper method to fix the experimental animal, uniformly coating (or attaching) the tested sample on the unhairing area of the experimental animal, coating the unhairing area as thin and uniform as possible, covering the unhairing area with a plastic film or cellophane and two layers of gauze, fixing the unhairing area with a non-irritating adhesive plaster or bandage to ensure that the tested sample is closely contacted with the skin and prevent the unhairing area from falling off and the animal licking the tested sample, generally sealing the unhairing area for 24 hours, removing the fixture and the covering after 24 hours, and washing the residual tested sample on the skin with warm water or a proper solvent. Observing the weight change of the experimental animal, and the like.

And (3) test results: the weight of the guinea pigs did not change significantly.

And (4) conclusion: the sample was judged to be mildly toxic according to transdermal toxicity classification, version ⒛ 15, of cosmetic safety specifications.

The concrete case is as follows: case 1: zhangqi, male age 58 years old and skin affected with eczema, was treated with the herbal bacteriostatic solution prepared in this example 2, and applied to the affected part once a day, morning and evening. Fig. 1-3 are the first day, the fourth day and the 7 th day of the skin condition pictures of the affected part of the patient, respectively, and it can be seen from fig. 3 that the eczema is better improved after the treatment for 7 days.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.

Claims

1. The herbal bacteriostatic liquid is characterized in that: comprises the following raw materials by mass: 3-8 g of lonicera confusa, 3-8 g of radix scutellariae, 3-8 g of oldenlandia diffusa, 3-8 g of big-mouth bowl and over 8 mu g/mL of pyrroloquinoline quinone liquid.

2. The herbal bacteriostatic liquid according to claim 1, which is characterized in that: the herbal bacteriostatic liquid comprises the following raw materials by mass: 5g of lonicera confusa, 5g of radix scutellariae, 5g of oldenlandia diffusa, 5g of big-mouth bowl and over 8 mu g/mL of pyrroloquinoline quinone liquid.

3. A preparation method of herbal antibacterial liquid is characterized by comprising the following steps: the specific method comprises the following steps: carefully selecting lonicera confusa, scutellaria baicalensis, oldenlandia diffusa and Chinese mugwort according to the mass raw material ratio of the herbal antibacterial liquid, drying, crushing into 80-100 mesh powder, extracting the liquid medicine components by using an extraction technology, adding pyrroloquinoline quinone liquid, and finally adding sterilized mineral water to prepare the antibacterial liquid.