CN111543425A

CN111543425A - Stem cell is deposited with refrigeration cryopreserving device

Info

Publication number: CN111543425A
Application number: CN202010585295.9A
Authority: CN
Inventors: 杨慧慧
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-06-23
Filing date: 2020-06-23
Publication date: 2020-08-18

Abstract

The invention discloses a refrigerating and freezing device for stem cell storage, which comprises a supporting seat and a hook rod, wherein a taking and placing box is arranged in the supporting seat, a moving wheel is arranged at the lower end of the supporting seat, a device body is arranged at the upper end of the supporting seat, an inner shell and liquid nitrogen are arranged in the device body, the liquid nitrogen is arranged between the inner shell and the device body, a controller is arranged at the outer side of the device body, a temperature sensor is arranged on the inner side wall of the device body, and an early warning signal lamp is arranged at the upper end of the device body. This stem cell is deposited and is used refrigeration cryopreserved device, annular array is distributing string mouthful groove and categorised label on the extension board frame, and it can be used for hanging of storage rack to put fixed the use to hang mouthful groove, freezes the sample information according to the stem cell that different storage racks were deposited, can carry out the information remark on corresponding categorised label, the differentiation of the heterogeneous stem cell cryopreserved sample of being convenient for is provided with second heat insulating layer and third heat insulating layer, under the effect on second heat insulating layer and third heat insulating layer, reduces the cold volume consumption of liquid nitrogen.

Description

Stem cell is deposited with refrigeration cryopreserving device

Technical Field

The invention relates to the technical field of stem cells, in particular to a refrigerating and freezing device for stem cell storage.

Background

Stem cells are a type of pluripotent cells with self-replication ability, under certain conditions, stem cells can be differentiated into various functional cells, and can be divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells, and can be divided into: the all-purpose stem cell, multipotential stem cell and unipotent stem cell, along with the development of science and technology and medical science and technology, people are more obvious to the importance understanding of stem cell, gather the stem cell, it can be used to the medical aspect to save after the processing, the stem cell is preserved and need be used the cryopreserved device, deposit the effect for keeping the stem cell, utilize liquid nitrogen to save the stem cell refrigeration usually, common cryopreserved device all is deposited the sample with the stem cell in device mouth department, when the liquid nitrogen in the cryopreserved device is not enough, need take out the sample with the stem cell cryopreserved just conveniently to add the liquid nitrogen usually, it is comparatively troublesome, because the developmental potential and the developmental stage of stem cell are different, need effectively distinguish the stem cell of cryopreserved, avoid obscuring.

Disclosure of Invention

The invention aims to provide a refrigerating cryopreservation device for stem cell storage, which solves the problems that the conventional cryopreservation device provided in the background art stores a stem cell cryopreservation sample at the device opening, when liquid nitrogen in the cryopreservation device is insufficient, the stem cell cryopreservation sample is usually required to be taken out to conveniently add the liquid nitrogen, the cryopreservation device is troublesome, and the frozen stem cells need to be effectively distinguished due to different development potentials and development stages of the stem cells, so that confusion is avoided.

In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a stem cell is deposited with refrigeration cryopreserving device, includes supporting seat and couple pole, be provided with in the supporting seat and get and put the box, and the supporting seat lower extreme is provided with and removes the wheel, the supporting seat upper end is provided with the device body, and installs internal casing and the liquid nitrogen of being provided with, and the liquid nitrogen setting is between the internal casing and the device body including the device simultaneously, the external side of device is provided with the controller, and installs the internal lateral wall and be provided with the temperature-sensing ware, and the internal upper end of device is provided with early warning.

Preferably, interior casing upper end runs through the internal top of device and is connected with sealed lid, and interior casing inside wall is provided with a supporting plate frame, seted up the pan feeding mouth on the supporting plate frame and hang the mouth groove, and the pan feeding mouth sets up the inboard at hanging the mouth groove, supporting plate frame upper surface is provided with categorised label, and just categorised label setting is in hanging the mouth groove outside, the couple pole is hung to on hanging the mouth groove, and the one end that the couple pole kept away from hanging the mouth groove runs through the pan feeding mouth and is connected with the storage rack, the storage rack up end is provided with the information label, and has seted up the hole of settling on the storage rack, and the hole inside wall of settling is provided with the protection pad.

Preferably, the supporting seat comprises a lower connecting plate, a connecting shell, a spring and an upper connecting plate, the lower end of the lower connecting plate is provided with the connecting shell, the upper end of the lower connecting plate is provided with the spring, the upper end face of the spring is connected with the upper connecting plate, and the upper connecting plate is provided with a device body.

Preferably, the taking and placing box comprises a first shell, a first heat insulation layer, a second shell and a carrying handle, the carrying handle is rotatably connected to the outer side of the first shell, the first heat insulation layer is arranged on the inner side of the first shell, and the second shell is arranged on the inner side of the first heat insulation layer.

Preferably, the device body includes a third casing, a fourth casing and a second heat insulation layer, the fourth casing is arranged on the inner side of the third casing, and the second heat insulation layer is arranged between the fourth casing and the third casing.

Preferably, the sealing cover comprises a third heat insulation layer, a first mounting groove, a first sealing ring, a second mounting groove and a second sealing ring, the third heat insulation layer is arranged at the top end in the sealing cover, the first mounting groove and the second mounting groove are arranged on the sealing cover, the first sealing ring is arranged in the first mounting groove, and the second sealing ring is arranged in the second mounting groove.

Preferably, the support plate frame is provided with hanging opening grooves in an annular array, and each hanging opening groove corresponds to one hook rod.

Preferably, the classification labels are distributed on the support plate frame in an annular array shape, and the classification labels correspond to the hanging opening grooves one to one.

Preferably, the arrangement holes are distributed on the storage rack in an annular array, and each arrangement hole corresponds to one information label.

Compared with the prior art, the invention has the beneficial effects that: the refrigeration freezing device for storing the stem cells,

(1) the freezing device is matched with a taking and placing box, the taking and placing box is used for taking, placing and transferring stem cell freezing samples in the device body, the second heat insulation layer reduces the influence of external temperature on the transferring process of the stem cell freezing samples, and moving wheels are arranged to facilitate the position moving of the device body;

(2) the device is characterized in that an inner shell is arranged in the device body, the inside of the inner shell is used for storing a stem cell cryopreservation sample, liquid nitrogen is filled between the device body and the inner shell and used for refrigerating and freezing the stem cell cryopreservation sample with the weight of the inner shell, and when the liquid nitrogen needs to be supplemented, the stem cell cryopreservation sample does not need to be taken out, and the liquid nitrogen can be directly supplemented into the device body;

(3) the liquid nitrogen early warning device is provided with a second heat insulation layer and a third heat insulation layer, the cold quantity consumption of liquid nitrogen is reduced under the action of the second heat insulation layer and the third heat insulation layer, and meanwhile, a temperature sensor, a controller and an early warning signal lamp are arranged;

(4) the support plate frame is provided with hanging opening grooves and classification labels in an annular array, the hanging opening grooves can be used for hanging and fixing storage racks, information can be remarked on the corresponding classification labels according to stem cell cryopreservation sample information stored in different storage racks, and differentiation of different types of stem cell cryopreservation samples is facilitated;

(5) the storage rack is distributed with information labels in an annular array, the information labels can correspond to stem cell storage tubes stored in the mounting holes, and the storage rack is used for standardizing other related information such as time of freezing and storing samples of the same type of stem cells and is convenient for subdividing the information labels.

Drawings

FIG. 1 is a schematic front sectional view of the present invention;

FIG. 2 is a schematic top view of the plate carrier of the present invention;

FIG. 3 is a schematic top view of the storage rack of the present invention;

FIG. 4 is an enlarged view of the structure at A in FIG. 1 according to the present invention;

FIG. 5 is a schematic view of the supporting seat structure of the present invention

FIG. 6 is a schematic view of a partial cross-sectional structure of the device body according to the present invention;

FIG. 7 is a schematic structural view of the connecting housing and the pick-and-place box according to the present invention;

FIG. 8 is a front view of the pick-and-place box of the present invention;

fig. 9 is a schematic view of the work flow structure of the present invention.

In the figure: 1. the device comprises a supporting seat, 101, a lower connecting plate, 102, a connecting shell, 103, a spring, 104, an upper connecting plate, 2, a taking and placing box, 201, a first shell, 202, a first heat insulation layer, 203, a second shell, 204, a carrying handle, 3, a moving wheel, 4, a device body, 401, a third shell, 402, a fourth shell, 403, a second heat insulation layer, 5, liquid nitrogen, 6, a controller, 7, a temperature sensor, 8, an early warning signal lamp, 9, an inner shell, 10, a sealing cover, 1001, a third heat insulation layer, 1002, a first mounting groove, 1003, a first sealing ring, 1004, a second mounting groove, 1005, a second sealing ring, 11, a support plate frame, 12, a feeding port, 13, a hanging port groove, 14, a classification label, 15, a hanging hook rod, 16, a storage frame, 17, an information label, 18, a placing hole, 19 and a protective pad.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1-9, the present invention provides a technical solution: a refrigeration freezing storage device for storing stem cells is disclosed, as shown in figure 1, figure 5, figure 6, figure 7, figure 8 and figure 9, a taking and placing box 2 is arranged in a supporting seat 1, a moving wheel 3 is arranged at the lower end of the supporting seat 1, the supporting seat 1 comprises a lower connecting plate 101, a connecting shell 102, a spring 103 and an upper connecting plate 104, the lower end of the lower connecting plate 101 is provided with the connecting shell 102, the upper end of the lower connecting plate 101 is provided with the spring 103, the upper end surface of the spring 103 is connected with the upper connecting plate 104, a device body 4 is arranged on the upper connecting plate 104, the influence generated by the moving vibration of the device is reduced under the action of the spring 103, the taking and placing box 2 is arranged in the connecting shell 102, the taking and placing box 2 comprises a first shell 201, a first heat insulation layer 202, a second shell 203 and a lifting handle 204, the outer side of the first shell 201 is rotatably connected with the lifting handle 204, the inner side of the first shell 201 is provided with the first, first heat insulating layer 202 reduces and receives the ambient temperature influence in the sample transfer process is deposited to stem cell freezing, supporting seat 1 upper end is provided with the device body 4, and be provided with interior casing 9 and liquid nitrogen 5 in the device body 4, while liquid nitrogen 5 sets up between interior casing 9 and the device body 4, the device body 4 is including third casing 401, fourth casing 402 and second heat insulating layer 403, and third casing 401 inboard is provided with fourth casing 402, be provided with second heat insulating layer 403 between fourth casing 402 and the third casing 401 simultaneously, second heat insulating layer 403 reduces the loss of the cold volume of the interior liquid nitrogen 5 of the device body 4, the device body 4 outside is provided with controller 6, and the device body 4 inside wall is provided with temperature-sensing ware 7, simultaneously the device body 4 upper end is provided with early warning signal lamp 8.

As shown in fig. 1, 2, 3 and 4, the upper end of the inner housing 9 is connected to the sealing cover 10 through the top end of the device body 4, and the inner side wall of the inner housing 9 is provided with a support plate frame 11, the sealing cover 10 includes a third thermal insulation layer 1001, a first mounting groove 1002, a first sealing ring 1003, a second mounting groove 1004 and a second sealing ring 1005, and the top end of the sealing cover 10 is provided with the third thermal insulation layer 1001, the sealing cover 10 is provided with the first mounting groove 1002 and the second mounting groove 1004, and the first sealing ring 1003 is arranged in the first mounting groove 1002, and the second sealing ring 1005 is arranged in the second mounting groove 1004, and the third thermal insulation layer 1001 reduces the loss of cold energy through the sealing cover 10, and the first sealing ring 1003 and the second sealing ring 1005 are arranged to enhance the sealing effect between the sealing cover 10 and the inner housing 9, and the support plate frame 11 is provided with a plurality of hanging grooves 13 in an annular array, and each hanging groove, the method comprises the steps of hanging a hook rod 15 on a supporting plate frame 11 through a hanging opening groove 13, fixedly placing a storage rack 16, arranging a feeding opening 12 and a hanging opening groove 13 on the supporting plate frame 11, arranging the feeding opening 12 on the inner side of the hanging opening groove 13, arranging a classification label 14 on the upper surface of the supporting plate frame 11, arranging the classification label 14 on the outer side of the hanging opening groove 13, arranging the classification labels 14 on the supporting plate frame 11 in an annular array shape, enabling the classification labels 14 to correspond to the hanging opening groove 13 one by one, carrying out information preparation on different types of stem cell cryopreserved samples through the classification labels 14 to facilitate different types of stem cells to be separately stored, hanging the hook rod 15 on the hanging opening groove 13, enabling one end, far away from the hanging opening groove 13, of the hook rod 15 to penetrate through the feeding opening 12 to be connected with the storage rack 16, arranging an information label 17 on the upper end surface of the storage rack 16, arranging a placing hole 18 on the storage rack 16, and arranging a protective pad 19 on the inner side wall, the placing holes 18 are distributed on the storage rack 16 in an annular array shape, each placing hole 18 corresponds to one information label 17, the stem cell storing tubes are fixed in the placing holes 18, the protective pad 19 plays a role in stabilizing, fixing and protecting the stem cell storing tubes, the information labels 17 can be used for detailed remarking according to the information of the freezing storage samples of the same type of stem cells, the freezing storage time and the like, and the stem cells of the same type on the same storage rack 16 can be conveniently distinguished and used.

The working principle is as follows: when the refrigeration freezing storage device for storing stem cells is used, the position of the device is moved and placed conveniently through the moving wheel 3, the stem cells to be stored are transferred to the device body 4 through the taking and placing box 2, the influence of external temperature on the transfer process of the stem cells is reduced by the first heat insulation layer 202, the power supply is switched on, the sealing cover 10 is opened, the hook rods 15 in the corresponding hanging opening grooves 13 are lifted under the action of the classification labels 14, the storage rack 16 is taken out through the feeding opening 12, the stem cell storage tubes in the taking and placing box 2 are placed in the placing holes 18, the protective pads 19 play a stable fixing and protecting effect on the stem cell storage tubes, the storage time information or other information of the stem cells can be noted on the information labels 17 to facilitate the distinguishing from other similar stem cells, after the stem cell storage tubes are placed, the storage rack 16 is placed in the inner shell 9 and hung in the hanging opening grooves 13 through the hook rods 15, after the stem cells are stored, the sealing cover 10 is covered, the sealing effect between the sealing cover 10 and the inner shell 9 is enhanced under the action of the first sealing ring 1003 and the second sealing ring 1005, the stem cells are refrigerated and stored under the action of liquid nitrogen 5 in the device body 4, the loss of cold energy is reduced under the action of the second heat-insulating layer 403 and the third heat-insulating layer 1001, the temperature sensor 7 detects the temperature in the device body 4, when the temperature sensor 7 transmits an electric signal to the controller 6, the model of the temperature sensor 7 is JWB-CW2000, the model of the controller 6 is KTS011, the controller 6 controls the early warning signal lamp 8 to be started, the early warning signal lamp can be used for prompting corresponding workers to supplement the liquid nitrogen, when the frozen stem cell samples need to be taken out, the sealing cover 10 is opened, the corresponding stem cell storage tube is taken out according to the information on the classification label 14 and the information label 17 and is placed in the taking and placing box 2 for transfer, the stem cells can be taken out and used, and the stem cells can be rewarming for 1-2 minutes by a water bath at 37 ℃, and the content which is not described in detail in the specification belongs to the prior art which is well known to a person skilled in the art.

The terms "central," "longitudinal," "lateral," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientation or positional relationship indicated in the drawings for simplicity of description only and are not intended to indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operative in a particular orientation, and are not to be considered limiting of the claimed invention.

Although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes in the embodiments and/or modifications of the invention can be made, and equivalents and modifications of some features of the invention can be made without departing from the spirit and scope of the invention.

Claims

1. The utility model provides a stem cell deposits and freezes device with refrigeration, includes supporting seat (1) and couple pole (15), its characterized in that: be provided with in supporting seat (1) and get and put box (2), and supporting seat (1) lower extreme is provided with and removes wheel (3), supporting seat (1) upper end is provided with the device body (4), and is provided with interior casing (9) and liquid nitrogen (5) in the device body (4), and liquid nitrogen (5) set up including between casing (9) and the device body (4) simultaneously, the device body (4) outside is provided with controller (6), and device body (4) inside wall is provided with temperature-sensing ware (7), and device body (4) upper end is provided with early warning signal lamp (8) simultaneously.

2. The refrigerated cryopreservation device for stem cell storage according to claim 1, wherein: the upper end of the inner shell (9) penetrates through the inner top end of the device body (4) and is connected with a sealing cover (10), and the inner side wall of the inner shell (9) is provided with a supporting plate frame (11), the supporting plate frame (11) is provided with a feeding port (12) and a hanging port groove (13), the feeding port (12) is arranged at the inner side of the hanging port groove (13), the upper surface of the supporting plate frame (11) is provided with a classification label (14), the classification label (14) is arranged at the outer side of the hanging opening groove (13), the hook rod (15) is hung on the hanging opening groove (13), and one end of the hook rod (15) far away from the hanging opening groove (13) penetrates through the feeding opening (12) to be connected with the storage rack (16), the upper end surface of the storage rack (16) is provided with an information label (17), and the storage rack (16) is provided with a placing hole (18), and the inner side wall of the placing hole (18) is provided with a protective pad (19).

3. The refrigerated cryopreservation device for stem cell storage according to claim 1, wherein: the supporting seat (1) comprises a lower connecting plate (101), a connecting shell (102), a spring (103) and an upper connecting plate (104), the lower end of the lower connecting plate (101) is provided with the connecting shell (102), the upper end of the lower connecting plate (101) is provided with the spring (103), the upper end face of the spring (103) is connected with the upper connecting plate (104), and meanwhile the upper connecting plate (104) is provided with a device body (4).

4. The refrigerated cryopreservation device for stem cell storage according to claim 1, wherein: the taking and placing box (2) comprises a first shell (201), a first heat insulation layer (202), a second shell (203) and a carrying handle (204), the carrying handle (204) is rotatably connected to the outer side of the first shell (201), the first heat insulation layer (202) is arranged on the inner side of the first shell (201), and the second shell (203) is arranged on the inner side of the first heat insulation layer (202).

5. The refrigerated cryopreservation device for stem cell storage according to claim 1, wherein: the device body (4) comprises a third shell (401), a fourth shell (402) and a second heat-insulating layer (403), the fourth shell (402) is arranged on the inner side of the third shell (401), and the second heat-insulating layer (403) is arranged between the fourth shell (402) and the third shell (401).

6. The refrigerated cryopreservation device for stem cell storage according to claim 2, wherein: the sealing cover (10) comprises a third heat insulation layer (1001), a first mounting groove (1002), a first sealing ring (1003), a second mounting groove (1004) and a second sealing ring (1005), the third heat insulation layer (1001) is arranged at the top end in the sealing cover (10), the sealing cover (10) is provided with the first mounting groove (1002) and the second mounting groove (1004), the first sealing ring (1003) is arranged in the first mounting groove (1002), and the second sealing ring (1005) is arranged in the second mounting groove (1004).

7. The refrigerated cryopreservation device for stem cell storage according to claim 2, wherein: hanging opening grooves (13) are distributed on the supporting plate frame (11) in an annular array mode, and each hanging opening groove (13) corresponds to one hanging hook rod (15).

8. The refrigerated cryopreservation device for stem cell storage according to claim 2, wherein: the classification labels (14) are distributed on the support plate frame (11) in an annular array shape, and the classification labels (14) correspond to the hanging opening grooves (13) one by one.

9. The refrigerated cryopreservation device for stem cell storage according to claim 2, wherein: the arrangement holes (18) are distributed on the storage rack (16) in an annular array shape, and each arrangement hole (18) corresponds to one information label (17).