CN213355293U

CN213355293U - Biological tissue sample freezes deposits device

Info

Publication number: CN213355293U
Application number: CN202022317989.2U
Authority: CN
Inventors: 赵雷; 况应敏; 朱月春; 倪月莉; 王超; 曾云俊
Original assignee: First Affiliated Hospital of Kunming Medical University
Current assignee: First Affiliated Hospital of Kunming Medical University
Priority date: 2020-10-16
Filing date: 2020-10-16
Publication date: 2021-06-04
Anticipated expiration: 2030-10-16

Abstract

The utility model discloses a cryopreservation device for biological tissue specimens, which comprises a body, a cover body, a specimen storage cup, a cryopreservation pipe, a clapboard and a bracket; the inside of the body is hollow to form an accommodating cavity, an opening is formed in the top of the body, and the cover body covers the opening of the body; the sample storage cup comprises a cup body and a plug, wherein the plug can be used for detachably filling a top opening of the cup body; the freezing tube is placed in the sample storage cup; the partition plate is arranged in the accommodating cavity and divides the accommodating cavity into a first cavity and a second cavity; the rack located in the first chamber or the second chamber is mounted on the partition plate, and the sample storage cup is detachably disposed on the rack. The utility model provides a biological tissue sample's cryopreserving device, structural design is reasonable, and it is very convenient to use, not only makes things convenient for freezing of biological tissue sample to deposit, remove and carry, is favorable to practicing thrift the cryopreserving liquid moreover to practice thrift the cost.

Description

Biological tissue sample freezes deposits device

Technical Field

The utility model relates to the technical field of medical equipment, concretely relates to biological tissue sample freeze and deposit device.

Background

The specimen is animal, plant, mineral and other matter, and may be preserved for long time and maintained as original appearance for exhibition, demonstration, education, identification, examination and other research.

In order to further study the structural characteristics or biochemical characteristics of biological tissue specimens, it is often necessary to store the biological tissue specimens at low temperature, freeze the specimens, and then take the specimens for use as needed to study the biological tissues.

However, the existing cryopreservation device for biological tissue specimens is a freezing tank with a large volume, and is very inconvenient to move and carry. When the number of the frozen biological tissue samples is small, the freezing tank needs to store more freezing fluid to freeze the biological tissue samples, which causes a problem of wasting the freezing fluid.

SUMMERY OF THE UTILITY MODEL

Therefore, the utility model provides a biological tissue sample freezes deposits device to solve the removal that is used for biological tissue sample's freezes to deposit the device among the prior art and carry very inconvenient problem.

In order to achieve the above object, the present invention provides the following technical solutions:

the utility model provides a biological tissue sample freezes deposits device, include:

the portable multifunctional electric heating cooker comprises a body and a cover body, wherein the inside of the body is hollow to form an accommodating cavity, an opening is formed in the top of the body, and the cover body covers the opening of the body;

a specimen storage cup including a cup body and a plug that removably fills a top opening of the cup body;

the freezing tube is placed in the sample storage cup;

the partition plate is arranged in the accommodating cavity and divides the accommodating cavity into a first cavity and a second cavity;

the bracket positioned in the first chamber or the second chamber is arranged on the partition plate, and the sample storage cup is detachably arranged on the bracket.

Optionally, a hanging hole is formed in the upper portion of the partition plate, and the bracket is hung on the partition plate through the hanging hole.

Optionally, the number of the suspension holes is multiple, and two brackets respectively located in the first chamber and the second chamber are suspended on each suspension hole.

Optionally, two freezing tubes are placed in each sample storage cup.

Optionally, the partition is detachably connected to the body.

Optionally, a through hole is formed in the lower portion of the partition plate, and the first chamber and the second chamber are communicated through the through hole.

Optionally, the number of through-going holes is 3-5.

Optionally, the through-going hole has a diameter of 0.5cm to 1 cm.

Optionally, the height of the sample storage cup is 20cm-30cm, the outer diameter of the cup body is 8cm-10cm, and the inner diameter of the cup body is 5cm-7 cm.

Optionally, the cover body sequentially comprises an insulating layer, an intermediate layer and a polymeric foam layer from top to bottom;

the outer side wall of the middle layer is provided with external threads, the inner side wall of the body is provided with internal threads matched with the external threads, and the middle layer is in threaded connection with the body.

The utility model has the advantages of as follows:

the utility model provides a biological tissue specimen freezing device, firstly, the biological tissue specimen is placed in a freezing tube; secondly, placing the freezing storage tube in a sample storage cup; thirdly, placing the sample storage cup in the body; finally, a freezing fluid, such as liquid nitrogen, is added to the interior of the body. The cryopreservation liquid can be used for cryopreserving the biological tissue specimens in the cryopreservation tube, and the operation is very convenient.

Because this device is deposited to freezing of biological tissue sample, simple structure, the volume is less, is convenient for remove and carry. Moreover, when the biological tissue sample that needs to freeze up is less, can place the sample that places biological tissue sample and deposit the cup and place in first cavity or second cavity, then add the frozen stock solution in first cavity or second cavity, this makes and can select the use amount of frozen stock solution according to the frozen stock quantity of biological tissue sample to help practicing thrift the use of frozen stock solution.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.

The structure, ratio, size and the like shown in the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by people familiar with the technology, and are not used for limiting the limit conditions which can be implemented by the present invention, so that the present invention has no technical essential significance, and any structure modification, ratio relationship change or size adjustment should still fall within the scope covered by the technical content disclosed by the present invention without affecting the efficacy and the achievable purpose of the present invention.

Fig. 1 is a schematic structural view of a cryopreservation apparatus for biological tissue specimens according to an embodiment of the present invention;

fig. 2 is a side view of a cryopreservation apparatus for biological tissue specimens, according to an embodiment of the present invention;

fig. 3 is a schematic structural view of a sample storage cup of the cryopreservation apparatus for biological tissue specimens provided in an embodiment of the present invention;

in the figure: in the figure: 1. a body; 11. a first chamber; 12. a second chamber; 2. a cover body; 21. an insulating and heat-insulating layer; 22. an intermediate layer; 23. a polymeric foam layer; 3. a sample storage cup; 31. a cup body; 32. a plug; 4. a partition plate; 41. a hanging hole; 42. a through hole; 5. a support; 6. and (5) freezing and storing the tube.

Detailed Description

The present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Example 1

As shown in fig. 1 to 3, the present embodiment provides a cryopreservation apparatus for a biological tissue specimen, which is used for cryopreservation of a biological tissue specimen.

Specifically, the cryopreservation device for the biological tissue specimen comprises a body 1, a cover body 2, a sample storage cup 3, a cryopreservation tube 6, a partition plate 4 and a bracket 5. Wherein, the inside cavity of body 1 forms and holds the chamber, holds the chamber and is used for holding parts such as support 5, baffle 4, sample storage cup. The top of body 1 sets up the opening, and sample storage cup, support 5 etc. put into through the top opening of body 1 and hold the intracavity. The cover body 2 covers the opening of the body 1, and the cover body 2 seals the top opening of the body 1 to prevent the frozen stock solution contained in the cup body 31 from volatilizing.

Further specifically, the specimen storage cup 3 includes a cup body 31 and a stopper 32, the stopper 32 detachably fills the top opening of the cup body 31, and the stopper 32 seals the top opening of the cup body 31. The freezing tube 6 is placed in the sample storage cup 3. The partition plate 4 is arranged in the accommodating cavity and divides the accommodating cavity into a first cavity 11 and a second cavity 12, and the partition plate 4 is used for dividing the space of the accommodating cavity and supporting and fixing the bracket 5. The bracket 5 located in the first chamber 11 or the second chamber 12 is mounted on the partition 4. The support 5 may be located in the first chamber 11 or in the second chamber 12. The position where the support 5 is placed can be selected as desired. The sample storage cup 3 is detachably arranged on the bracket 5, so that the sample storage cup 3 is convenient to take.

The cryopreservation device for biological tissue specimens provided by the embodiment is used as follows: firstly, placing a biological tissue specimen in a freezing tube 6; secondly, placing the freezing tube 6 in the sample storage cup 3; thirdly, placing the sample storage cup 3 in the body 1; finally, a freezing liquid, for example liquid nitrogen, is added to the interior of the body 1. The cryopreservation liquid can be used for cryopreserving the biological tissue specimen placed in the cryopreservation tube 6, and the operation is very convenient.

Because this device is deposited to freezing of biological tissue sample, simple structure, the volume is less, is convenient for remove and carry. Moreover, when the biological tissue specimens which need to be frozen are few, the sample storage cup 3 in which the biological tissue specimens are placed can be placed in the first chamber 11 or the second chamber 12, and then the frozen stock solution is added in the first chamber 11 or the second chamber 12, which enables the amount of the frozen stock solution to be selected according to the number of the frozen biological tissue specimens, thereby contributing to saving the use of the frozen stock solution.

In addition, the cryopreservation tube 6 can be placed in the sample storage cup 3, then a cryopreservation liquid, such as liquid nitrogen, is added into the sample storage cup 3, the biological tissue specimen in the cryopreservation tube 6 is subjected to cryopreservation by the cryopreservation liquid in the sample storage cup, and the biological tissue specimen is moved and carried by transferring the sample storage cup.

Optionally, a hanging hole 41 is provided at an upper portion of the partition plate 4, and the bracket 5 is hung from the partition plate 4 through the hanging hole 41. The bracket 5 is hung on the partition board 4 through the hanging hole 41, so that the operation is very simple, and the bracket 5 is convenient to fix.

Optionally, the number of the hanging holes 41 is multiple, and two brackets 5 respectively located in the first chamber 11 and the second chamber 12 are hung on each hanging hole 41. One hanging hole 41 can hang two brackets 5, thereby facilitating the fixing of the brackets 5. The rack 5 may be placed in the first chamber 11, or the second chamber 12, or the first chamber 11 and the second chamber 12, respectively, depending on the number of the frozen biological tissue specimens.

For example, when the number of the frozen biological tissue specimens is small, the rack 5 may be placed in the first chamber 11 or the second chamber 12 separately to reduce the amount of the frozen stock solution to save the cost. When the number of the frozen biological tissue specimens is large, a plurality of the racks 5 may be placed in the first chamber 11 and the second chamber 12, respectively, to increase the frozen number of the biological tissue specimens.

Optionally, two freezing tubes 6 are placed in each sample storage cup 3. The freezing tube 6 is placed in the sample storage cup 3, so that the freezing tube 6 can be conveniently stored. The sample storage cup 3 is used for transferring the cryopreservation tube 6, so that the transfer and taking of the biological tissue specimen are facilitated, and the operation is very simple.

Optionally, the partition 4 is detachably connected to the body 1. This facilitates the mounting and dismounting of the partition 4.

Optionally, a through hole 42 is formed in a lower portion of the partition plate 4, and the first chamber 11 and the second chamber 12 communicate with each other through the through hole 42. When the quantity of the biological tissue sample that needs freezing to deposit is more, all placed freezing in first cavity 11 and the second cavity 12 and deposit pipe 6, and first cavity 11 and second cavity 12 pass through hole 42 intercommunication, and the liquid level balance of the freezing solution in the body 1 of being convenient for is convenient for deposit the freezing of biological tissue sample.

Optionally, the number of the through holes 42 is 3 to 5. This enables the pressures in the first and

second chambers

11, 12 to be rapidly equalized when the frozen stock solution is added to the body 1, facilitating the maintenance of the stability of the partition 4 fixed to the body 1.

Optionally, the diameter of the through hole 42 is 0.5cm to 1 cm. This helps to quickly achieve liquid level equilibrium of the first chamber 11 and the second chamber 12 on both sides of the through-hole 42.

Optionally, the height of the sample storage cup 3 is 20cm-30cm, the outer diameter of the cup body 31 is 8cm-10cm, and the inner diameter of the cup body 31 is 5cm-7 cm. The sample storage cup 3 is helpful for storing the cryopreservation tube 6, and is convenient for storing the biological tissue specimen in the cryopreservation tube 6.

Optionally, the cover 2 comprises an insulating layer 21, an intermediate layer 22 and a polymeric foam layer 23 from top to bottom.

The outer side wall of the middle layer 22 is provided with external threads, the inner side wall of the body 1 is provided with internal threads matched with the external threads, and the middle layer 22 is in threaded connection with the body 1.

The insulating layer 21 helps to maintain a low temperature environment in the body 1, facilitating cryopreservation of the biological tissue specimen. The middle layer 22 facilitates the connection and fixation between the lid body 2 and the cup body 31, and the polymeric foam layer 23 can better seal the top opening of the body 1, and can effectively prevent the frozen stock solution in the body 1 from volatilizing.

The cryopreservation device for the biological tissue specimens, provided by the embodiment, has the advantages of reasonable structural design and very convenient use, is not only convenient for cryopreservation, movement and carrying of the biological tissue specimens, but also is favorable for saving cryopreservation liquid, and thus the cost is saved.

In this embodiment, this freezing device volume is less, and the quality is light, and portable transports freezes the deposit liquid quantity simultaneously and is few, can practice thrift the cost. In addition, the device conveniently realizes freezing of biological tissue sample at any time and deposits, is convenient for storage and transportation of biological tissue sample, and it has reduced the cost of transportation who carries the liquid nitrogen container of great volume when transporting, has avoided causing the influence to the cell of biological tissue sample in the transportation simultaneously.

Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. A cryopreservation device for biological tissue specimens, comprising:

the freezing tube is placed in the sample storage cup;

2. The cryopreservation device for the biological tissue specimen according to claim 1, wherein a hanging hole is formed in an upper portion of the partition plate, and the scaffold is hung on the partition plate through the hanging hole.

3. The apparatus according to claim 2, wherein the number of the hanging holes is plural, and two brackets are hung on each hanging hole and respectively located in the first chamber and the second chamber.

4. The cryopreservation device for biological tissue specimens as claimed in claim 3,

two freezing storage tubes are arranged in each sample storage cup.

5. The device of claim 1, wherein the septum is removably coupled to the body.

6. The cryopreservation device for biological tissue specimens according to claim 5, wherein a through hole is provided at a lower portion of the partition plate, and the first chamber and the second chamber are communicated through the through hole.

7. The cryopreservation device for biological tissue specimens according to claim 6, wherein the number of the through holes is 3 to 5.

8. The cryopreservation device for biological tissue specimens according to claim 6, wherein the diameter of the through hole is 0.5cm-1 cm.

9. The cryopreservation device for biological tissue specimens as claimed in claim 6, wherein the height of the specimen storage cup is 20cm to 30cm, the outer diameter of the cup body is 8cm to 10cm, and the inner diameter of the cup body is 5cm to 7 cm.

10. The cryopreservation device for the biological tissue specimen according to claim 1, wherein the cover body comprises an insulating and heat-insulating layer, an intermediate layer and a polymeric foam layer in sequence from top to bottom;