CN216887808U

CN216887808U - Cell is with freezing device of depositing

Info

Publication number: CN216887808U
Application number: CN202220216807.9U
Authority: CN
Inventors: 姜强; 徐珂; 徐文作
Original assignee: Nanjing Sansheng Biotechnology Co ltd
Current assignee: Nanjing Sansheng Biotechnology Co ltd
Priority date: 2022-01-26
Filing date: 2022-01-26
Publication date: 2022-07-05
Anticipated expiration: 2032-01-26

Abstract

The utility model discloses a cell cryopreservation device, and particularly relates to the technical field of cell cryopreservation. The cell freezing device has the advantages of reasonable structural design, strong heat preservation effect, convenience in replacing freezing liquid, guarantee of the freezing environment of cells, buffer effect and capability of avoiding the cell storage tube from cracking during placement.

Description

Cell is with freezing device of depositing

Technical Field

The utility model relates to the technical field of cell cryopreservation, in particular to a cell cryopreservation device.

Background

Cell cryopreservation is a technology for placing cells in a low-temperature environment to reduce cell metabolism so as to facilitate long-term storage, and deep low-temperature cryopreservation is the most important form of long-term storage of the cells. The cells are stored in liquid nitrogen at the temperature of-196 ℃ by using a freezing technology at a low temperature, so that the cells can be temporarily separated from a growth state to store the cell characteristics, and the cells are revived for application when needed. The cell deep low temperature storage technology has important significance in the advanced fields of biomedicine, such as seed cell storage, human immune cell and stem cell storage, cell therapy drug development and the like, and is one of important basic means for life health research.

Generally place the cell in the cell storage tube among the prior art, then place the cell storage tube in freezing the case of depositing, nevertheless because do not have better protective structure and limit structure in freezing the case, the storage tube easily takes place to contact with freezing the case when carrying out placing of cell storage tube, probably causes the cell storage tube to take place to break. The present device was invented based on the above problems.

SUMMERY OF THE UTILITY MODEL

In order to overcome the defects in the prior art, the utility model provides a cell cryopreservation device, which solves the problem that the cell storage tube is likely to be broken due to the fact that the storage tube is easily contacted with a cryopreservation box when the existing cell storage tube is placed.

In order to achieve the above purpose, the utility model provides the following technical scheme: the utility model provides a cell is with freezing device, includes interior storage bucket and cell storage tube, a plurality of standing groove one has been seted up on the interior storage bucket, the cell storage tube is established in standing groove one, the bottom fixed mounting of standing groove one has compression spring, compression spring's top fixed mounting has the foam-rubber cushion, the outside of interior storage bucket is equipped with outer storage bucket, standing groove two has been seted up on the outer storage bucket, interior storage bucket is established in standing groove two, and the diameter of standing groove two is greater than the external diameter of interior storage bucket, the outside of interior storage bucket is equipped with first heat preservation, the reservoir has been seted up to the inside of outer storage bucket, be equipped with the frozen stock liquid in the reservoir.

In a preferred embodiment, the diameter of the first placing groove is greater than that of the cell storage tube, the length of the cell storage tube is greater than that of the first placing groove, so that a worker can take the cell storage tube out conveniently, the cross section of the spongy cushion is U-shaped, so that the spongy cushion can hold the cell storage tube conveniently, and the height of the inner storage barrel is smaller than that of the outer storage barrel.

In a preferred embodiment, a liquid inlet hole is formed in the upper surface of one side of the outer storage barrel, a liquid inlet pipe is fixedly mounted in the liquid inlet hole, a sealing plug is arranged at the pipe opening of the liquid inlet pipe, and the sealing plug is pulled out to facilitate a worker to pour new frozen stock solution.

In a preferred embodiment, a liquid outlet hole is formed in the lower end of one side of the outer storage barrel, a liquid outlet pipe is fixedly mounted in the liquid outlet hole, a one-way valve is arranged on the liquid outlet pipe, and frozen liquid can be discharged by opening the one-way valve.

In a preferred embodiment, the outer surface of the outer storage barrel is provided with an oblique mounting hole, a temperature detector is arranged in the oblique mounting hole, and a worker can observe the temperature of the frozen stock solution in the outer storage barrel in real time according to the temperature detector, so that the frozen stock solution is prevented from influencing the frozen stock of cells after the temperature of the frozen stock solution rises.

In a preferred embodiment, the top end of the outer storage barrel is hinged with a barrel cover, the barrel cover is made of transparent materials, so that a worker can observe the freezing condition of cells when the barrel cover is not opened, and a lifting handle is fixedly arranged on the upper surface of one side of the barrel cover.

In a preferred embodiment, an annular installation groove is formed in the outer storage barrel, the annular installation groove is formed in the outer side of the liquid storage tank, a second heat preservation cushion is fixedly installed in the annular installation groove, and the second heat preservation cushion prevents the temperature of the frozen stock solution from losing.

In a preferred embodiment, the first heat-insulating pad and the second heat-insulating pad are both made of polyurethane foam boards, so that the loss of the temperature of the frozen stock solution is effectively avoided.

Compared with the prior art, the utility model has the beneficial effects that:

1. according to the cell storage tube, the compression spring and the spongy cushion are arranged, so that the cell storage tube can be supported by the spongy cushion when the cell storage tube is stored, and meanwhile, the compression spring and the spongy cushion can play a certain buffering effect, so that the cell storage tube is prevented from being broken due to the fact that the bottom end of the cell storage tube is in contact with the bottom end of the first placing groove, and certain economic loss is caused;

2. according to the utility model, by arranging the liquid inlet pipe, the liquid outlet pipe and the thermometer, when the cell storage pipe is placed, a worker can observe the temperature of the frozen stock solution in the outer storage barrel in real time, when the temperature of the frozen stock solution changes, the worker can open the one-way valve to allow the frozen stock solution with the changed temperature to flow out, and open the sealing plug and pour in new frozen stock solution after the frozen stock solution is completely flowed, so that the frozen stock environment of cells is ensured, and the cells are ensured to still have cell characteristics when being separated from a growth state.

Drawings

For a clearer explanation of the embodiments of the present application or technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to the drawings.

FIG. 1 is a first general structural diagram of the present invention;

FIG. 2 is a second overall view of the present invention;

FIG. 3 is a top view of the present invention with the inner storage barrel removed;

FIG. 4 is a cross-sectional view of the outer storage bucket of the present invention;

FIG. 5 is a top view of the inner storage bucket and cell storage tube of the present invention;

fig. 6 is a cross-sectional view of fig. 5 of the present invention.

Description of reference numerals:

1. an inner storage barrel; 2. an outer storage bucket; 3. a cell storage tube; 4. a compression spring; 5. a sponge cushion; 6. a first thermal insulation pad; 7. a liquid storage tank; 8. a liquid inlet pipe; 9. a sealing plug; 10. a temperature detector; 11. a barrel cover; 12. lifting and pulling the handle; 13. a liquid outlet pipe; 14. a one-way valve; 15. a second heat-insulating pad.

Detailed Description

The present invention is described in terms of particular embodiments, other advantages and features of the utility model will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the utility model and that it is not intended to limit the utility model to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to the attached drawings 1-6 of the specification, the freezing and storing device for the cells of the embodiment comprises an inner storage barrel 1 and a cell storage tube 3, wherein a plurality of first placing grooves are formed in the inner storage barrel 1, the cell storage tube 3 is arranged in the first placing grooves, the diameter of the first placing grooves is larger than that of the cell storage tube 3, so that the cell storage tube 3 is limited, the length of the cell storage tube 3 is larger than that of the first placing grooves, the top end of the cell storage tube 3 is higher than the top surface of the inner storage barrel 1, so that a worker can conveniently take out the cell storage tube 3, a compression spring 4 is fixedly arranged at the bottom of the first placing grooves, a sponge mat 5 is fixedly arranged at the top end of the compression spring 4, the cross section of the sponge mat 5 is U-shaped, the inner diameter of the sponge mat 5 is larger than that of the cell storage tube 3, so that the cell storage tube 3 is held, realize buffering effect, the outside of interior storage bucket 1 is equipped with outer storage bucket 2, the height that highly is less than outer storage bucket 2 of interior storage bucket 1, cell storage tube 3 and bung 11's interior top surface emergence contact when avoiding closing bung 11, standing groove two has been seted up on outer storage bucket 2, interior storage bucket 1 is established in standing groove two, and the diameter of standing groove two is greater than the external diameter of interior storage bucket 1, the outside of interior storage bucket 1 is equipped with first heat preservation and fills up 6, reservoir 7 has been seted up to the inside of outer storage bucket 2, be equipped with the frozen stock solution in the reservoir 7.

The top end of the outer storage barrel 2 is hinged with a barrel cover 11, the barrel cover 11 is made of transparent materials, so that a worker can directly observe the freezing condition of cells on the premise of not opening the barrel cover 11, a lifting handle 12 is fixedly arranged on the upper surface of one side of the barrel cover 11, and the worker can open the barrel cover 11 quickly and take out the cell storage tube 3 conveniently.

The annular mounting groove has been seted up on outer storage bucket 2, the annular mounting groove is established in the outside of reservoir 7, fixed mounting has the second heat preservation to fill up 15 in the annular mounting groove, and second heat preservation fills up 15 can prevent that the temperature of freezing stock solution from scattering and disappearing, first heat preservation fills up 6 and second heat preservation fills up 15 and all adopts polyurethane foam board to make, and polyurethane foam is a novel synthetic material that has heat preservation and waterproof performance, and the coefficient of thermal conductivity is low, scattering and disappearing of avoiding the temperature that can be fine.

The implementation scenario is specifically as follows: in the using process of the utility model, a worker opens the barrel cover 11, puts the cell storage tube 3 with cells into the first placing groove arranged on the inner storage barrel 1, and the compression spring 4 and the U-shaped spongy cushion 5 are arranged in the first placing groove, so that the spongy cushion 5 can support the cell storage tube 3 when the cell storage tube 3 falls in, and the compression spring 4 and the spongy cushion 5 can play a certain buffering effect, thereby avoiding the rupture of the cell storage tube 3 caused by the contact between the bottom end of the cell storage tube 3 and the bottom end of the first placing groove when the worker places the cell storage tube 3, and causing a certain economic loss.

Referring to the attached drawings 1-4 of the specification, in the freezing device for cells of the embodiment, a liquid inlet hole is formed in the upper surface of one side of an outer storage barrel 2, a liquid inlet pipe 8 is fixedly installed in the liquid inlet hole, a sealing plug 9 is arranged at the pipe orifice of the liquid inlet pipe 8, the sealing plug 9 is pulled out to facilitate pouring of new freezing liquid, the sealing plug 9 is arranged to prevent foreign matters from falling into a second placing groove, a liquid outlet hole is formed in the lower end of one side of the outer storage barrel 2, a liquid outlet pipe 13 is fixedly installed in the liquid outlet hole, a one-way valve 14 is arranged on the liquid outlet pipe 13, the freezing liquid can be discharged by opening the one-way valve 14, an inclined mounting hole is formed in the outer surface of the outer storage barrel 2, a temperature measurer 10 is arranged in the inclined mounting hole, a worker can observe the temperature of the freezing liquid in the outer storage barrel 2 in real time according to the temperature measurer 10 to prevent the temperature of the freezing liquid from being increased to influence the freezing of the cells, when the temperature of the cryopreservation liquid changes, a worker can quickly open the one-way valve 14 to enable the cryopreservation liquid with the changed temperature to flow out, open the sealing plug 9 after the cryopreservation liquid flows out, and pour in new cryopreservation liquid, so that the temperature of the cryopreservation liquid is prevented from changing when cells are cryopreserved, the cryopreservation environment of the cells is guaranteed, and the cells are guaranteed to have cell characteristics when being separated from the growth state.

And finally: the above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the utility model, and any modifications, equivalents, improvements and the like that are within the spirit and principle of the present invention are intended to be included in the scope of the present invention.

Claims

1. The utility model provides a cell is with freezing device that deposits, includes interior storage bucket (1) and cell storage tube (3), its characterized in that: the cell storage tube is characterized in that a plurality of first placing grooves are formed in the inner storage barrel (1), the cell storage tube (3) is arranged in the first placing grooves, compression springs (4) are fixedly arranged at the bottom of the first placing grooves, sponge cushions (5) are fixedly arranged at the top ends of the compression springs (4), outer storage barrels (2) are arranged on the outer sides of the inner storage barrel (1), second placing grooves are formed in the outer storage barrels (2), the inner storage barrel (1) is arranged in the second placing grooves, the diameter of the second placing grooves is larger than the outer diameter of the inner storage barrel (1), first heat preservation cushions (6) are arranged on the outer sides of the inner storage barrel (1), liquid storage grooves (7) are formed in the outer storage barrel (2), and frozen liquid is arranged in the liquid storage grooves (7).

2. The cryopreservation device for cells according to claim 1, wherein: the diameter of the first placing groove is larger than that of the first cell storage tube (3), the length of the first cell storage tube (3) is larger than that of the first placing groove, the cross section of the spongy cushion (5) is U-shaped, and the height of the inner storage barrel (1) is smaller than that of the outer storage barrel (2).

3. The cryopreservation device for cells according to claim 1, wherein: a liquid inlet hole is formed in the upper surface of one side of the outer storage barrel (2), a liquid inlet pipe (8) is fixedly mounted in the liquid inlet hole, and a sealing plug (9) is arranged at the pipe opening of the liquid inlet pipe (8).

4. The cryopreservation device for cells according to claim 1, wherein: a liquid outlet hole is formed in the lower end of one side of the outer storage barrel (2), a liquid outlet pipe (13) is fixedly mounted in the liquid outlet hole, and a one-way valve (14) is arranged on the liquid outlet pipe (13).

5. The cryopreservation device for cells according to claim 1, wherein: an inclined mounting hole is formed in the outer surface of the outer storage barrel (2), and a temperature detector (10) is arranged in the inclined mounting hole.

6. The cryopreservation device for cells according to claim 1, wherein: the top end of the outer storage barrel (2) is hinged with a barrel cover (11), the barrel cover (11) is made of transparent materials, and a lifting handle (12) is fixedly mounted on the upper surface of one side of the barrel cover (11).

7. The cryopreservation device for cells according to claim 1, wherein: an annular mounting groove is formed in the outer storage barrel (2), the annular mounting groove is formed in the outer side of the liquid storage tank (7), and a second heat-insulating pad (15) is fixedly mounted in the annular mounting groove.

8. The cryopreservation device for cells according to claim 7, wherein: the first heat-preservation pad (6) and the second heat-preservation pad (15) are both made of polyurethane foam boards.