CN111500471B - Repairing agent for treating cyanide-containing tailing slag - Google Patents
Repairing agent for treating cyanide-containing tailing slag Download PDFInfo
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- CN111500471B CN111500471B CN202010389236.4A CN202010389236A CN111500471B CN 111500471 B CN111500471 B CN 111500471B CN 202010389236 A CN202010389236 A CN 202010389236A CN 111500471 B CN111500471 B CN 111500471B
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- culture solution
- rhodotorula glutinis
- aspergillus oryzae
- pseudomonas putida
- cyanide
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- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 title claims abstract description 56
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 36
- 239000002893 slag Substances 0.000 title abstract description 26
- 241000223253 Rhodotorula glutinis Species 0.000 claims abstract description 40
- 241000589776 Pseudomonas putida Species 0.000 claims abstract description 16
- 240000006439 Aspergillus oryzae Species 0.000 claims abstract description 14
- 235000002247 Aspergillus oryzae Nutrition 0.000 claims abstract description 14
- 239000000243 solution Substances 0.000 claims description 67
- 238000002156 mixing Methods 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 28
- 238000012258 culturing Methods 0.000 claims description 19
- 241000589516 Pseudomonas Species 0.000 claims description 18
- 239000000843 powder Substances 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 15
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- 239000010445 mica Substances 0.000 claims description 12
- 229910052618 mica group Inorganic materials 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 11
- 230000008439 repair process Effects 0.000 claims description 11
- 239000002609 medium Substances 0.000 claims description 10
- 238000011081 inoculation Methods 0.000 claims description 9
- ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 8
- 229910000013 Ammonium bicarbonate Inorganic materials 0.000 claims description 8
- 235000012538 ammonium bicarbonate Nutrition 0.000 claims description 8
- 239000001099 ammonium carbonate Substances 0.000 claims description 8
- 239000008187 granular material Substances 0.000 claims description 8
- 239000002245 particle Substances 0.000 claims description 8
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 8
- 239000007864 aqueous solution Substances 0.000 claims description 7
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 6
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 238000000643 oven drying Methods 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- 150000001875 compounds Chemical class 0.000 claims description 5
- 238000005067 remediation Methods 0.000 claims description 5
- 229920002261 Corn starch Polymers 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000008120 corn starch Substances 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 4
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 claims description 4
- 238000005550 wet granulation Methods 0.000 claims description 4
- 244000005700 microbiome Species 0.000 abstract description 14
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 abstract description 13
- 239000010931 gold Substances 0.000 abstract description 13
- 229910052737 gold Inorganic materials 0.000 abstract description 13
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 239000000969 carrier Substances 0.000 abstract description 4
- 230000015556 catabolic process Effects 0.000 description 11
- 238000006731 degradation reaction Methods 0.000 description 11
- 239000007788 liquid Substances 0.000 description 9
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 8
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 238000001354 calcination Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000000227 grinding Methods 0.000 description 5
- 230000000813 microbial effect Effects 0.000 description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 description 4
- 238000001816 cooling Methods 0.000 description 4
- 238000005507 spraying Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 240000003183 Manihot esculenta Species 0.000 description 3
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 3
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 238000006065 biodegradation reaction Methods 0.000 description 3
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000194107 Bacillus megaterium Species 0.000 description 2
- 241000194103 Bacillus pumilus Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- 241000606860 Pasteurella Species 0.000 description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 229910021536 Zeolite Inorganic materials 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229940046414 biotin 1 mg Drugs 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 239000004567 concrete Substances 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 2
- 229910000396 dipotassium phosphate Inorganic materials 0.000 description 2
- 235000019797 dipotassium phosphate Nutrition 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 235000013379 molasses Nutrition 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- JMANVNJQNLATNU-UHFFFAOYSA-N oxalonitrile Chemical compound N#CC#N JMANVNJQNLATNU-UHFFFAOYSA-N 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- 239000010457 zeolite Substances 0.000 description 2
- 240000005007 Actinomucor elegans Species 0.000 description 1
- 235000013650 Actinomucor elegans Nutrition 0.000 description 1
- 241000588986 Alcaligenes Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000604 Hydrolases Proteins 0.000 description 1
- 239000005909 Kieselgur Substances 0.000 description 1
- 108010033272 Nitrilase Proteins 0.000 description 1
- 241000187654 Nocardia Species 0.000 description 1
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 1
- 241000589540 Pseudomonas fluorescens Species 0.000 description 1
- 241000589614 Pseudomonas stutzeri Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- KKTCWAXMXADOBB-UHFFFAOYSA-N azanium;hydrogen carbonate;hydrate Chemical compound [NH4+].O.OC([O-])=O KKTCWAXMXADOBB-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000004566 building material Substances 0.000 description 1
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 1
- 239000000292 calcium oxide Substances 0.000 description 1
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 1
- 239000004568 cement Substances 0.000 description 1
- 238000003889 chemical engineering Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 210000001822 immobilized cell Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000002825 nitriles Chemical class 0.000 description 1
- 229910000510 noble metal Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- CHWRSCGUEQEHOH-UHFFFAOYSA-N potassium oxide Chemical compound [O-2].[K+].[K+] CHWRSCGUEQEHOH-UHFFFAOYSA-N 0.000 description 1
- 229910001950 potassium oxide Inorganic materials 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000012266 salt solution Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- KKCBUQHMOMHUOY-UHFFFAOYSA-N sodium oxide Chemical compound [O-2].[Na+].[Na+] KKCBUQHMOMHUOY-UHFFFAOYSA-N 0.000 description 1
- 229910001948 sodium oxide Inorganic materials 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
- B09B3/10—Destroying solid waste or transforming solid waste into something useful or harmless involving an adsorption step
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- C12N1/14—Fungi; Culture media therefor
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- C12N1/16—Yeasts; Culture media therefor
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- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/91—Use of waste materials as fillers for mortars or concrete
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Abstract
The invention belongs to the technical field of environmental protection, and discloses a repairing agent for treating cyanide-containing tailing slag, which comprises a carrier, rhodotorula glutinis, pseudomonas putida and aspergillus oryzae. The repairing agent is prepared by matching microorganisms and carriers, has strong environmental friendliness, does not produce secondary pollution, and can quickly remove cyanide of the gold tailings.
Description
Technical Field
The invention belongs to the technical field of environmental protection, and relates to a repairing agent for treating cyanide-containing tailing slag.
Background
The waste residue discharged after the gold concentrate is extracted from the ore is the gold tailings. Gold production generally adopts a cyaniding gold extraction method, so that a certain amount of cyanogen is also remained in tailings, and the cyanides belong to highly toxic substances, so that the utilization of the tailings is limited. The storage mode occupies a large amount of land and pollutes surface water. The high-temperature incineration method can oxidize and decompose the organic matters, but the method generates secondary pollution to the environment, has large equipment investment and high energy consumption, and enterprises need to invest large cost and are difficult to popularize and use. Some enterprises also produce tailings as building materials, for example, document 1: chinese patent CN103319132A discloses a method for producing concrete by using tailings, which is to treat tailings through screening and calcining processes, mix the tailings with cement, coarse sand and other materials to prepare concrete. The microbial remediation method belongs to an in-situ remediation technology, has the advantages of low remediation cost, no secondary pollution and the like, and has some research achievements in the prior art.
Document 2: CN 108220197A discloses a gold tailing slag microorganism composite treatment application method, belonging to the field of road engineering. The method combines the pasteurella with one or more of bacillus subtilis, bacillus pumilus or bacillus megaterium. Calcium carbonate crystals are generated by depositing the pasteurella bacillus in pores of the gold tailings, so that the mechanical property and stability of the calcium carbonate crystals are improved, and heavy metal and noble metal ions in the tailings are fixed; decomposing cyanide in the gold tailings by using bacillus subtilis, bacillus pumilus or bacillus megaterium; the cyanide contained in the slag can be reduced from 423mg/kg initially to 4.84mg/kg after 30 days by the method.
Document 3: CN 105057314A discloses an in-situ bioremediation treatment method of cyanide-containing tailings. After removing impurities and crushing and grinding, the cyanide-containing tailing slag is mixed with soil and crushed straws, inorganic salt solution is sprayed to adjust the water content and the pH value of the tailing slag, then the inoculation of cyanide-containing bacteria is carried out, the cyanide-reducing flora is one or the combination of more of pseudomonas fluorescens, pseudomonas putida, pseudomonas stutzeri, nocardia and bacillus, and then the cyanide-containing tailing slag is placed into an aeration system for ventilation treatment. The cyanide content of the slag can be reduced from the initial 413mg/kg to 4.36mg/kg after 30 days by this method.
However, the methods disclosed in the above documents do not disclose specific combinations of strains, and different combinations of strains may produce competitive antagonism, and even if no antagonistic competitive relationship is produced, the effect difference is large, and the methods only list combinations among a plurality of different genera, do not list specific strains, and do not have specific combinations, and the general technical scheme is vague, and it is difficult for enterprises to implement large-scale effective implementation.
Document 4: CN 107509915A discloses a method for reducing cyanide content in cassava residue, which comprises mixing yeast and actinomucor elegans according to a volume ratio of 1-5:1-3 to obtain a mixed bacterial liquid, then mixing 10-20mL of the mixed bacterial liquid with 180 g of cassava residue, and fermenting at 25-31 ℃ for 48-96 h; however, the method can only degrade the cassava residues with lower cyanide content of 10mg/kg, and cannot be applied to the tailing residues with high cyanide concentration and harsh nutritional conditions.
Document 5: experimental study on cyanide degradation by zeolite immobilized cells, in 2005 of the college chemical engineering report, the method fixes the alcaligenes on the zeolite and is applied to degradation of waste water containing cyanide under the laboratory condition. The method is in a laboratory stage, is only suitable for repairing cyanide wastewater, and has poor repairing effect on the tailing slag containing cyanide, mainly because the difference between the liquid and solid microbial repairing environments is large, and the nutritional conditions in the tailing slag are harsh.
How to carry out rapid and thorough biodegradation on tailing slag cyanide is a technical problem which needs to be solved urgently. In biodegradation, selection of proper microorganisms is particularly important, usually a single microorganism is difficult to realize, proper microorganisms need to be screened according to properties among the microorganisms for compatibility, and a compatibility relationship is verified through a specific test so as to achieve a technical effect of symbiotic synergy.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide a repairing agent for treating cyanide-containing tailings, which is biodegradable, has strong environmental friendliness, does not produce secondary pollution and can quickly remove the cyanide of gold tailings.
In order to achieve the technical purpose, the invention adopts the following technical scheme:
the repairing agent for treating cyanide-containing tailings is characterized by comprising rhodotorula glutinis, pseudomonas putida and aspergillus oryzae.
Specifically, the repairing agent is prepared according to the following method: mixing Rhodotorula glutinis culture solution and carrier, culturing under anaerobic condition for 6-10h, adding mixed culture solution of Pseudomonas putida and Aspergillus oryzae, culturing under aerobic condition for 10-15h, and oven drying at low temperature until water content is 6-10% to obtain the repairing agent.
Further, the rhodotorula glutinis culture solution: carrier: the proportion of the pseudomonas putida-aspergillus oryzae mixed culture solution is 1L: 1-3 kg: 1L of the total amount of the active ingredients.
Further, the carrier comprises diatomite and mica powder.
Preferably, the rhodotorula glutinis culture solution: carrier: the proportion of the pseudomonas putida-aspergillus oryzae mixed culture solution is 1L: 2 kg: 1L of the compound.
Preferably, the carrier is prepared according to the following method: mixing diatomite and mica powder uniformly according to the mass ratio of 1-2:1-2, and then mixing the diatomite and the mica powder according to the mass ratio of 1-2 kg: adding 8-15% (w/v) ammonium bicarbonate aqueous solution at the ratio of 2-3L, stirring uniformly, performing wet granulation to obtain wet granules with the particle size of 100-.
More preferably, the carrier is prepared according to the following method: mixing diatomite and mica powder uniformly according to the mass ratio of 1:1, and then mixing the components according to the weight ratio of 2 kg: adding 10% (w/v) ammonium bicarbonate aqueous solution at a ratio of 3L, stirring uniformly, performing wet granulation to obtain wet granules with a particle size of 100-.
More preferably, the preparation method of the rhodotorula glutinis culture solution comprises the following steps: inoculating the Rhodotorula glutinis seed solution into Rhodotorula glutinis fermentation medium according to the inoculation amount of 8%, and culturing at constant temperature of 32 deg.C for 48h to obtain Rhodotorula glutinis culture solution.
More preferably, the components of the fermentation medium are: 20g of glucose, 10g of corn starch, 5g of monopotassium phosphate, 2g of magnesium sulfate heptahydrate, 0.1g of manganese sulfate monohydrate, 0.1g of ferrous sulfate heptahydrate and the balance of water, wherein the volume is fixed to 1L, and the pH value is adjusted to 7.0.
More preferably, the preparation method of the pseudomonas putida-aspergillus oryzae mixed culture solution comprises the following steps:
mixing the pseudomonas putida seed solution and the aspergillus oryzae seed solution according to the ratio of 1:1 to obtain a mixed seed solution; and inoculating the mixed seed solution into a fermentation culture medium according to the inoculation amount of 10%, and culturing at the constant temperature of 30 ℃ for 24 hours to obtain the pseudomonas putida-aspergillus oryzae mixed culture solution.
The beneficial effects of the invention mainly comprise the following aspects:
the repairing agent is prepared by matching microorganisms and carriers, has strong environmental friendliness, does not produce secondary pollution, and can quickly remove cyanide of the gold tailings.
The diatomite has good compatibility with most microorganisms and can be used as an attachment carrier of the microorganisms; the mica powder contains a large amount of silicate and can adsorb cyanide; the microorganism carrier containing open pores is prepared by granulating and calcining the diatomite, the mica powder and the ammonium bicarbonate, so that the microorganism carrier is favorable for the attachment of microorganisms and can adsorb cyanide in an exchange environment. The components of the cyanide are organic C and N, so that carbon and nitrogen in the outside are limiting factors for degrading the cyanide by microorganisms, and the biodegradation of the cyanide-containing waste in the environment can be hindered.
Rhodotorula glutinis belongs to facultative anaerobic microorganisms, can survive under aerobic and anaerobic conditions, contains nitrilase for degrading cyanide, can quickly remove low-concentration cyanide, but the enzyme activity is inhibited by high-concentration cyanide, so that the microbial organisms are inhibited from biodegrading compounds; the invention firstly carries out anaerobic adsorption treatment on the rhodotorula glutinis and a carrier, so that the rhodotorula glutinis is attached to the hole environment.
Pseudomonas putida contains cyanide hydrolase, can utilize cyanide as a nutrient to generate ammonia and carbon dioxide, and resists toxicity of cyanide at high concentration, but has the defect of slow degradation rate; aspergillus oryzae is capable of adsorbing cyanide and is an effective microbial adsorbent and co-acts with Pseudomonas putida, which both co-treat cyanide.
The microbe repairing agent can quickly repair high-concentration cyanide tailings, firstly aspergillus oryzae and a carrier adsorb cyanide in the environment to the surface of the repairing agent, pseudomonas putida can degrade the cyanide to generate ammonia and carbon dioxide, the cyanide concentration on the surface of the repairing agent is reduced, rhodotorula glutinis can quickly degrade residual cyanide, and therefore quick repair of the high-concentration cyanide tailings is achieved.
Drawings
FIG. 1: the effect of different remediation agents on cyanide degradation rate;
FIG. 2: the effect of the repair time on the cyanide degradation rate.
Detailed Description
Those skilled in the art can modify the process parameters appropriately to achieve the desired results with reference to the disclosure herein. It is expressly intended that all such similar substitutes and modifications which would be obvious to one skilled in the art are deemed to be included in the invention. While the products and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations and modifications, or appropriate alterations and combinations, of the products and methods described herein may be made and utilized without departing from the spirit, scope, and spirit of the invention. For a further understanding of the present invention, reference will now be made in detail to the following examples.
The starting materials or reagents used in the present invention are commercially available unless otherwise specified. The rhodotorula glutinis specifically ATCC32765, the pseudomonas putida specifically ATCC11172 and the aspergillus oryzae specifically ATCC20423 are used in the invention.
Example 1
A process for screening and repairing tailings comprising the steps of:
mechanically stirring the tailing slag, grinding, sieving, controlling the granularity to be below 1mm, collecting undersize slag powder, and grinding the oversize slag again;
adding the repairing agent into 500 times of 30 ℃ water by weight, and stirring for 10min at 200rpm to obtain biological repairing liquid;
according to the biological repair liquid: slag powder = 1L: spraying bioremediation liquid into the slag powder in a proportion of 5kg, stirring while spraying, uniformly mixing, performing bioremediation at 28 ℃ for 10 days, turning over once every other day, detecting the water content, and controlling the water content to be not lower than 15%.
The repairing agent is prepared according to the following process:
mixing diatomite and mica powder uniformly according to the mass ratio of 1:1, and then mixing the components according to the weight ratio of 2 kg: adding 10% (w/v) ammonium bicarbonate aqueous solution at a ratio of 3L, stirring, wet granulating to obtain wet granules with a particle size of 300 μm, calcining at 450 deg.C for 30min, taking out, and naturally cooling to room temperature to obtain carrier;
mixing Rhodotorula glutinis culture solution and carrier, culturing under anaerobic condition for 8 hr, adding mixed culture solution of Pseudomonas putida and Aspergillus oryzae, culturing under aerobic condition for 12 hr, oven drying at 20 deg.C until water content is 8%, and refrigerating at 4 deg.C to obtain repairing agent; the rhodotorula glutinis culture solution: carrier: the proportion of the pseudomonas putida-aspergillus oryzae mixed culture solution is 1L: 2 kg: 1L of the compound.
The preparation method of the rhodotorula glutinis culture solution comprises the following steps:
mixing Rhodotorula glutinis seed solution (concentration of 10% 8 cfu/ml) is inoculated into a rhodotorula glutinis fermentation medium according to the inoculation amount of 8 percent, and the rhodotorula glutinis is cultured for 48 hours at the constant temperature of 32 ℃ to obtain a rhodotorula glutinis culture solution; the fermentation medium comprises the following components: 20g of glucose, 10g of corn starch, 5g of monopotassium phosphate, 2g of magnesium sulfate heptahydrate, 0.1g of manganese sulfate monohydrate, 0.1g of ferrous sulfate heptahydrate and the balance of water, wherein the volume is fixed to 1L, and the pH value is adjusted to 7.0.
The preparation method of the pseudomonas putida-aspergillus oryzae mixed culture solution comprises the following steps:
separately culturing Pseudomonas putida and Aspergillus oryzae to a concentration of 10 9 cfu/ml of seed solution, and then mixing the mixture according to the ratio of 1:1 to obtain a mixed seed solution; inoculating the mixed seed solution into a fermentation culture medium according to the inoculation amount of 10%, and culturing at the constant temperature of 30 ℃ for 24 hours to obtain a pseudomonas putida-aspergillus oryzae mixed culture solution; the fermentation medium comprises the following components: 30g of molasses, 10g of urea, 1g of monopotassium phosphate, 1g of dipotassium phosphate, 0.5g of calcium carbonate, 0.1g of magnesium sulfate heptahydrate and VB 1 5mg and biotin 1mg, the volume is adjusted to 1L, and the pH value is adjusted to 6.5.
Example 2
A process for screening and repairing tailings comprising the steps of:
mechanically stirring the tailing slag, grinding, sieving, controlling the granularity to be below 1mm, collecting undersize slag powder, and grinding the oversize slag again;
adding the repairing agent into 400 times of 35 ℃ water by weight, and stirring at 200rpm for 10min to obtain biological repairing liquid;
according to the biological repair liquid: slag powder = 1L: spraying bioremediation liquid into the slag powder according to the proportion of 6kg, stirring while spraying, uniformly mixing, placing at 32 ℃ for bioremediation for 10d, turning over once every other day, detecting the water content, and controlling the water content to be not less than 15%.
The repairing agent is prepared according to the following process:
mixing diatomite and mica powder uniformly according to the mass ratio of 2:1, and then mixing the diatomite and the mica powder according to the weight ratio of 1 kg: adding 12% (w/v) ammonium bicarbonate aqueous solution at a ratio of 2L, stirring, wet granulating to obtain wet granules with a particle size of 500 μm, calcining at 500 deg.C for 30min, taking out, and naturally cooling to room temperature to obtain carrier;
mixing Rhodotorula glutinis culture solution and carrier, culturing for 6h under anaerobic condition, adding mixed culture solution of Pseudomonas putida and Aspergillus oryzae, culturing for 12h under aerobic condition, oven drying at 20 deg.C until water content is 12%, and refrigerating at 4 deg.C to obtain repairing agent; the rhodotorula glutinis culture solution: carrier: the proportion of the pseudomonas putida-aspergillus oryzae mixed culture solution is 1L: 2 kg: 1L of the compound.
The preparation method of the rhodotorula glutinis culture solution comprises the following steps:
mixing Rhodotorula glutinis seed solution (concentration of 10% 8 cfu/ml) is inoculated into a rhodotorula glutinis fermentation medium according to the inoculation amount of 8 percent, and the rhodotorula glutinis is cultured for 48 hours at the constant temperature of 32 ℃ to obtain a rhodotorula glutinis culture solution; the fermentation medium 1L comprises the following components: 20g of glucose, 10g of corn starch, 5g of monopotassium phosphate, 2g of magnesium sulfate heptahydrate, 0.1g of manganese sulfate monohydrate, 0.1g of ferrous sulfate heptahydrate and the balance of water, wherein the pH value is 7.0.
The preparation method of the pseudomonas putida-aspergillus oryzae mixed culture solution comprises the following steps:
separately culturing Pseudomonas putida and Aspergillus oryzae to a concentration of 10 9 cfu/ml of seed solution, and then mixing the seed solution according to the ratio of 1:1 to obtain a mixed seed solution; inoculating the mixed seed solution into a fermentation culture medium according to the inoculation amount of 10%, and culturing at constant temperature of 30 ℃ for 24h to obtain a pseudomonas putida-aspergillus oryzae mixed culture solution; the components of the fermentation medium 1L are as follows: 30g of molasses, 10g of urea, 1g of monopotassium phosphate, 1g of dipotassium phosphate, 0.5g of calcium carbonate, 0.1g of magnesium sulfate heptahydrate and VB 1 5mg, biotin 1mg, pH 6.5.
Comparative example 1
The same as example 1; the difference lies in that the carriers in the repairing agent are different, and the preparation process is as follows:
to diatomaceous earth, 2 kg: adding 10% (w/v) ammonium bicarbonate aqueous solution at a ratio of 3L, stirring, wet granulating to obtain wet granules with a particle size of 300 μm, calcining at 450 deg.C for 30min, taking out, and naturally cooling to room temperature to obtain the carrier.
Comparative example 2
The same as example 1; the difference lies in that the carriers in the repairing agent are different, and the preparation process comprises the following steps:
adding 2kg of mica powder: adding 10% (w/v) ammonium bicarbonate water solution at a ratio of 3L, stirring, wet granulating to obtain wet granules with particle size of 300 μm, calcining at 450 deg.C for 30min, taking out, and naturally cooling to room temperature to obtain the carrier.
Comparative example 3
The same as example 1; the difference lies in that the bacteria in the repairing agent are different, and the preparation process is as follows:
mixing Rhodotorula glutinis culture solution and carrier, culturing under anaerobic condition for 8 hr, oven drying at 20 deg.C until water content is 8%, and refrigerating at 4 deg.C to obtain repairing agent; the rhodotorula glutinis culture solution: the proportion of the carrier is 1L: 1 kg.
Comparative example 4
The same as example 1; the difference lies in that the bacteria in the repairing agent are different, and the preparation process is as follows:
mixing the Pseudomonas putida-Aspergillus oryzae mixed culture solution with a carrier, culturing for 12h under aerobic condition, drying at low temperature of 20 deg.C until the water content is 8%, and refrigerating at 4 deg.C to obtain repairing agent; the pseudomonas putida-aspergillus oryzae mixed culture solution comprises the following components: the proportion of the carrier is 1L: 1 kg.
Comparative example 5
Mixing Rhodotorula glutinis culture solution, Pseudomonas putida-Aspergillus oryzae mixed culture solution and carrier, culturing under aerobic condition for 12 hr, oven drying at 20 deg.C until water content is 8%, and refrigerating at 4 deg.C to obtain repairing agent; the rhodotorula glutinis culture solution: carrier: the proportion of the pseudomonas putida-aspergillus oryzae mixed culture solution is 1L: 2 kg: 1L of the total amount of the active ingredients.
Comparative example 6
No bioremediation treatment was used as a blank control.
Example 3
The tailing slag is gold ore slag obtained after gold is extracted by a cyaniding gold extraction method, and mainly comprises the following components: 71.5% of silicon dioxide, 11.2% of aluminum oxide, 5.9% of potassium oxide, 5.8% of sodium oxide and 2.3% of calcium oxide; total cyanide CN T The content is 491 mg/kg.
The contents of cyanide in the repaired slag were analyzed to calculate the degradation rates of cyanide in examples 1-2 and comparative examples 1-6, and as shown in FIG. 1, the total cyanide contents in the treatment groups of examples 1-2 were reduced to 2.46mg/kg and 4.41mg/kg, and the degradation rates reached 99.5% and 99.1%, respectively, which were significantly better than those in comparative examples 1-5. The microbial repairing agent of the embodiment 1-2 can efficiently and quickly repair high-concentration cyanide tailings, firstly aspergillus oryzae and a carrier adsorb cyanide in the environment to the surface of the repairing agent, pseudomonas putida can degrade the cyanide to generate ammonia and carbon dioxide, the concentration of the cyanide on the repairing agent is reduced, and at the moment, rhodotorula glutinis can quickly degrade residual cyanide, so that the quick repair of the high-concentration cyanide tailings is realized.
Taking examples 1-2 as examples, the repair time is selected to be 2,4,6,8,10,12,14, and the time unit is d as a detection point, as shown in fig. 2, the cyanide degradation rate is rapidly increased with the increase of the repair time, the degradation rate of 6d reaches about 50%, the degradation rate of 8d is maintained between 80-90%, and the degradation rate of 10d reaches over 99%.
Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.
Claims (5)
1. The repairing agent for treating cyanide-containing tailings is characterized by being prepared by the following method: mixing Rhodotorula glutinis culture solution and carrier, culturing under anaerobic condition for 6-10h, adding mixed culture solution of Pseudomonas putida and Aspergillus oryzae, culturing under aerobic condition for 10-15h, and oven drying at low temperature until water content is 6-15% to obtain repairing agent;
the rhodotorula glutinis culture solution: carrier: the proportion of the pseudomonas putida-aspergillus oryzae mixed culture solution is 1L: 1-3 kg: 1L;
the carrier is prepared according to the following method: mixing diatomite and mica powder uniformly according to the mass ratio of 1-2:1-2, and then mixing the components according to the weight ratio of 1-2 kg: adding 8-15% w/v ammonium bicarbonate aqueous solution at a ratio of 2-3L, stirring uniformly, performing wet granulation to obtain wet granules with a particle size of 100-;
the preparation method of the pseudomonas putida-aspergillus oryzae mixed culture solution comprises the following steps: mixing the pseudomonas putida seed solution and the aspergillus oryzae seed solution according to the proportion of 1:1 to obtain a mixed seed solution; and (3) inoculating the mixed seed solution into a fermentation culture medium according to the inoculation amount of 10%, and culturing at the constant temperature of 30 ℃ for 24h to obtain the pseudomonas putida-aspergillus oryzae mixed culture solution.
2. The repair agent according to claim 1, wherein the Rhodotorula glutinis culture solution: carrier: the proportion of the pseudomonas putida-aspergillus oryzae mixed culture solution is 1L: 2 kg: 1L of the compound.
3. The repair agent according to claim 1, wherein the carrier is prepared by the following method: mixing diatomite and mica powder uniformly according to the mass ratio of 1:1, and then mixing the components according to the weight ratio of 2 kg: adding 10% w/v ammonium bicarbonate aqueous solution at a ratio of 3L, stirring uniformly, performing wet granulation to obtain wet granules with a particle size of 100-.
4. The repairing agent according to claim 1, wherein the Rhodotorula glutinis culture solution is prepared by: inoculating the Rhodotorula glutinis seed solution into Rhodotorula glutinis fermentation medium according to the inoculation amount of 8%, and culturing at constant temperature of 32 deg.C for 48h to obtain Rhodotorula glutinis culture solution.
5. The remediation agent of claim 1 wherein said fermentation medium comprises the components of: 20g of glucose, 10g of corn starch, 5g of monopotassium phosphate, 2g of magnesium sulfate heptahydrate, 0.1g of manganese sulfate monohydrate, 0.1g of ferrous sulfate heptahydrate and the balance of water, wherein the volume is fixed to 1L, and the pH value is adjusted to 7.0.
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| CN105057314A (en) * | 2015-08-23 | 2015-11-18 | 长春黄金研究院 | In-situ bioremediation processing method for cyanide-contained tailing slag |
| CN107509915A (en) * | 2017-08-25 | 2017-12-26 | 广西壮族自治区水牛研究所 | A kind of method for reducing manioc waste cyanide content |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105057314A (en) * | 2015-08-23 | 2015-11-18 | 长春黄金研究院 | In-situ bioremediation processing method for cyanide-contained tailing slag |
| CN107509915A (en) * | 2017-08-25 | 2017-12-26 | 广西壮族自治区水牛研究所 | A kind of method for reducing manioc waste cyanide content |
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Denomination of invention: A Repairing Agent for Treatment of Cyanide Containing Tailings Effective date of registration: 20221118 Granted publication date: 20220906 Pledgee: Fuyang Zhejiang rural commercial bank Limited by Share Ltd. Pledgor: ZHEJIANG CHENGYU ENVIRONMENTAL PROTECTION NEW MATERIAL Co.,Ltd. Registration number: Y2022980022320 |