CN106479919A - A kind of sludge composting heavy metal passivation microbial inoculum and production method and application - Google Patents
A kind of sludge composting heavy metal passivation microbial inoculum and production method and application Download PDFInfo
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Abstract
A kind of sludge composting heavy metal is passivated microbial inoculum, is obtained by one or two fermentations of sulphate-reducing thermophilic bacterium Archaeoglobus veneficus VC 16 (DSM 4304), Thermodesulfobacterium commune YSRA 1 (DSM 2178)；Its production method step is：1) actication of culture, 2) seed liquor preparation, 3) solid fermentation；Present invention also offers sludge composting heavy metal is passivated the application of microbial inoculum；Beneficial effect is：This sludge composting heavy metal passivation microbial inoculum is heat-resist, enables the in-situ passivation of heavy metal, wide adaptability, safe, good passivation effect；Can be directly added in mud mixture material, process is simple, innoxious degree are high；Sulfate reduction can be carried out using Organic substance in mud and sulfate ion, the humification process of sludge composting and the fast degradation of organic pollution can be accelerated.
Description
Technical field
The invention belongs to organic solid waste disposal of resources field is and in particular to a kind of be used for weight during sludge composting
Metal passivating technique, especially relates to a kind of production and application being passivated microbial inoculum for the heavy metal during sludge composting.
Background technology
Municipal sludge is the precipitate of generation during municipal sewage treatment.China produces more than 3,000 ten thousand tons of mud per year, and
Increased with annual 15% speed.Municipal sludge is rich in the nutrient such as N, P, K and organic matter, is the organic fertilizer resource of high-quality.At present should
It is mainly high temperature aerobic composting technology for the city sewage sludge reclamation technology that organic commercial fertilizer produces, send out in high-temperature aerobic
The quick humification of Organic substance and stabilisation in mud under the conditions of ferment.But because in most mud, the heavy metal such as Cu, Pb, Cr, Cd contains
Amount is exceeded, and sludge composting heavy metals in process cannot be realized effectively controlling, and sludge composting Land_use change has huge environment
Risk.
At present, heavy metal recovery technique is mainly in-situ passivation technology, that is, add passivator and change in sludge composting process
The form of heavy metal (Cu, Pb, Cr, Cd), thus realize the in-situ passivation of heavy metal.Chemical inactivators high cost, secondary pollution
Seriously.The advantages of microorganism in situ passivating technique is because of low cost, non-secondary pollution becomes the first-selection of heavy metal passivating technique.
Heavy metal microorganism in situ passivating technique is using the absorption of certain micro-organisms heavy metal, precipitation, oxidoreduction
Deng effect, thus reducing toxicity and the animal migration of heavy metal.Sulfate reducting bacteria can form H sulfate reduction2S and with a huge sum of money
Belong to reaction and form precipitation, be widely used to heavy metal in water passivation and remove.It is passivated principle：In sulfate-reduction conditions
Under, sulfate reducting bacteria heavy metal in-situ passivation principle is mainly：1) discharge electronics in sulfate-reducing process or produce energy
Cu (II), Cr (VI) are reduced to Cu (I) and Cr by enough reduction Cu (II), metabolites of Cr (VI), electronics and this metabolite
(III), the bio-toxicity of heavy metal reduces；2) on the one hand sulfate reduction consumes hydrogen ion so that solution ph raises,
Metal ion is precipitated with hydroxide form, reduces the dispersal ability of heavy metal；3) hydrogen sulfide and metal ion react, raw
Become soluble metal sulfide, such as Cu2S, PbS, CdS etc., realize the in-situ passivation of heavy metal.Current ambient temperature sulfate reduction
Bacterium heavy metal biological recovery technique is extensively applied in water body, soil organic pollutant/heavy metal reparation.But due to sludge
During fertilizer temperature higher it is impossible to direct interpolation sulfate reducting bacteria heavy metal in-situ passivation microbial inoculum, therefore room temperature sulfate is also
Opportunistic pathogen heavy metal in-situ passivation microbial inoculum can only use during stage or ferment in second time are become thoroughly decomposed in mud ageing, and technique is more multiple
Miscellaneous.
Sulphate-reducing thermophilic bacterium has high temperature resistant and sulfate reduction correlation properties, can realize under the high temperature conditions
The reduction fixation of toxic heavy metal and the accelerated degradation of organic pollution.Have been found that the sulphate-reducing thermophilic of part in recent years
Bacterium, such as Archaeoglobus fulgidus CNCM I-3465 and Archaeoglobus fulgidus CNCM I-3469 etc.
Can be used for organic pollutant degradation.Through retrieval, not yet find to be directly used in sludge composting process using sulphate-reducing thermophilic bacterium
The report of heavy metal in-situ passivation.
Content of the invention
It is an object of the invention to overcoming the shortcomings of existing sludge composting disposal technology, using sulphate-reducing thermophilic bacterium
Sulfur reduction characteristic, provides a kind of sludge composting heavy metal passivation microbial inoculum and production method, and provides application process.
The sludge composting heavy metal passivation microbial inoculum that the present invention provides, by sulphate-reducing thermophilic bacterium Archaeoglobus
Veneficus VC-16 (DSM 4304), the one of Thermodesulfobacterium commune YSRA-1 (DSM 2178)
Plant or two kinds of fermentations are obtained.Described sulphate-reducing thermophilic bacterium Archaeoglobus veneficus VC-16 (DSM
4304), the strain of Thermodesulfobacterium commune YSRA-1 (DSM 2178) is protected from German microorganism fungus kind
Tibetan center DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen Gmbh, DSMZ)
Buy and obtain.Described sulphate-reducing thermophilic bacterium Archaeoglobus veneficus VC-16 (DSM 4304),
Thermodesulfobacterium commune YSRA-1 (DSM 2178) bio-safety grade is one-level.
Sludge composting heavy metal is passivated microbial inoculum, including sulphate-reducing thermophilic bacterium and adjuvant, it is characterized in that：Sludge composting weight
Metal is passivated the every g of the microbial inoculum total bacteria count of bacterium containing sulphate-reducing thermophilic more than 500,000,000, and described adjuvant includes fluid medium and solid-state is sent out
Ferment culture medium, than 5-10 part, described solid-state weight is than 90-95 part for described fluid medium weight；Described liquid culture based component
And formula is：Every 1000.00mL deionized water adds KCl 0.34g, MgCl2·6H2O 4.00g, MgSO4·7H2O 3.45g,
NH4Cl 0.25g, CaCl2·2H2O 0.14g, K2HPO40.14g, NaCl 18.00g, yeast extract 0.50g, sodium lactate 1.50g,
Fe(NH4)2(SO4)2·7H2O solution (0.1%w/v) 2.00mL, trace element solution 10.00mL, "diazoresorcinol" sodium salt solution
(0.1%w/v) 0.50mL, NaHCO33.00g, Na2S·9H2O 0.50g, deionized water 1000.00mL；Described trace element is molten
Liquid composition and formula are：Every 1000.00mL deionized water adds nitrilotriacetic acid 1.5g, MgSO4·7H2O3.0g, MnSO4·H2O
0.5g, NaCl 1.0g, FeSO4·7H2O 0.1g, CoCl2·6H2O 0.1g, CaCl20.1g, ZnSO4·7H2O 0.1g,
CuSO4·5H2O 0.01g, AlK (SO4)2·12H2O 0.01g, H3BO30.01g, Na2MoO4·2H2O0.01g；Described solid-state
Fermentation medium components and formula are：Powder of straw weight compares 5- than 20-30 part, municipal sludge weight than 20-30 part, wheat bran weight
10 parts, than 30-50 part, fluid medium weight is than 1-5 part for bean cake weight.
It is a further object of the present invention to provide described sludge composting heavy metal is passivated the production method of microbial inoculum, step is as follows：
1) actication of culture：Take sulfate reducting bacteria strain to be inoculated in culture medium, cultivate 5-10d under the conditions of 45-85 DEG C；Excellent
Choosing, Archaeoglobus veneficus VC-16 cultivation temperature is 80 DEG C, Thermodesulfobacterium
Commune YSRA-1 cultivation temperature is 70 DEG C；
2) seed liquor preparation：The strain of activation is inoculated in fluid medium, carries out one-level, two grades, three-level expansion training
Support；Condition of culture is 45-80 DEG C；Stop culture when culture fluid OD600 is between 1.8-3.0, obtain first order seed；By one-level kind
Son 10% is seeded in the shaking flask of more than 1L and carries out two grades of amplification culture, when culture fluid OD600 is between 1.8-3.0 by volume
When stop culture, obtain secondary seed solution；
3) solid fermentation：After amplification culture terminates, by the inoculum concentration of 5-10%, secondary seed solution seed liquor is accessed solid-state
Fermentation medium, 45-85 DEG C of fermentation culture 5-10d；After culture terminates, detection total bacteria count reaches 500,000,000/gram more than, pulverize and sieve,
Obtain final product sludge composting heavy metal passivation microbial inoculum；
Wherein, step 1) and step 2) described liquid culture based component is：KCl 0.34g, MgCl2·6H2O 4.00g,
MgSO4·7H2O 3.45g, NH4Cl 0.25g, CaCl2·2H2O 0.14g, K2HPO40.14g, NaCl 18.00g, yeast extract
0.50g, sodium lactate 1.50g, Fe (NH4)2(SO4)2·7H2O solution (0.1%w/v) 2.00mL, trace element solution
10.00mL, "diazoresorcinol" sodium salt solution (0.1%w/v) 0.50mL, NaHCO33.00g, Na2S·9H2O 0.50g, deionized water
1000.00mL, pH6.8-7.0；Described trace element solution composition is：Trace element solution composition is：Nitrilotriacetic acid 1.5g,
MgSO4·7H2O 3.0g, MnSO4·H2O 0.5g, NaCl 1.0g, FeSO4·7H2O 0.1g, CoCl2·6H2O 0.1g,
CaCl20.1g, ZnSO4·7H2O 0.1g, CuSO4·5H2O 0.01g, AlK (SO4)2·12H2O 0.01g, H3BO30.01g,
Na2MoO4·2H2O 0.01g, deionized water 1000mL；
Described sulfate reducting bacteria be Archaeoglobus veneficus VC-16,
Thermodesulfobacterium commune YSRA-1 one or two；
Step 3) described solid-state fermentation culture medium preparation method is：By powder of straw, municipal sludge, wheat bran and bean cake at a high speed
Pulverizer is pulverized, and crosses 60 mesh sieves, according to mass percent for 20-30: 20-30: 5-10: 30-50 ratio uniform mix homogeneously,
Add the fluid medium of 1-5%, 121 DEG C of sterilizings；Described fluid medium be step 1) in fluid medium consistent.
Present invention simultaneously provides described sludge composting heavy metal is passivated the application of microbial inoculum：
Add 1-5% sludge composting heavy metal passivation microbial inoculum carry out compost fermentation in sludge composting material, including with
Lower step：
1) composting material pretreatment：Composting material is that 20-40% is fluffy by the municipal sludge of moisture content 60-80% and moisture content
Pine material forms, and mud and fluff material weight are than for 5: 1-2, is 6.5-7.5 with adjusting PH with base value；Described fluff material is to have
Machine matter is wood flour, mushroom slag, straw, at least one in rice husk.
2) microbial inoculum inoculation：In 1-5% ratio inoculation heavy metal passivation microbial inoculum, mix.
3) high temperature aerobic composting：Mixture is carried out aerobic compost, after sludge composting temperature reaches 60 DEG C, daily turning
Once；Fermentation 20-40d.
4) crush and screen：After mud heap temperature is down to 35 DEG C or less than 35 DEG C, sludge composting is pulverized, sieve.
5) heavy metal passivation effect inspection：After the completion of mud composting, in detection sludge composting, Cu, Zn, Pb, Cr, Cd etc. have
Effect state content of beary metal, when available heavy metal content reduces by more than 70%, heavy metal passivation microbial inoculum meets quality standard.
The invention has the beneficial effects as follows：
The sludge composting heavy metal passivation microbial inoculum that the present invention provides is heat-resist, can be real during sludge high temperature composting
Its growth existing and propagation, and realize the in-situ passivation of heavy metal, have the features such as wide adaptability, safe, good passivation effect.
The sludge composting heavy metal passivation microbial inoculum that the present invention provides is directly added in mud mixture material and carries out high temperature composting,
Without secondary fermentation, have the advantages that process is simple, innoxious degree are high.
The sludge composting heavy metal passivation microbial inoculum that the present invention provides can be entered using Organic substance in mud and sulfate ion
Row sulfate reduction, can accelerate the humification process of sludge composting and the fast of organic pollution while realizing heavy metal passivation
Prompt drop solution.
Specific embodiment
Further describe the present invention with reference to specific embodiment.
Sludge composting heavy metal is passivated the production method of microbial inoculum：
Embodiment 1
A kind of sludge composting heavy metal is passivated the production method of microbial inoculum, comprises the following steps that：
1) actication of culture：Archaeoglobus veneficus VC-16 strain is taken to be inoculated in 100mL sulfate reducting bacteria
In liquid-liquid culture medium, cultivate 7d under the conditions of 80 DEG C.
Liquid culture based component is：KCl 0.34g, MgCl2·6H2O 4.00g, MgSO4·7H2O 3.45g, NH4Cl
0.25g, CaCl2·2H2O 0.14g, K2HPO40.14g, NaCl 18.00g, yeast extract 0.50g, sodium lactate 1.50g, Fe
(NH4)2(SO4)2·7H2O solution (0.1%w/v) 2.00mL, trace element solution 10.00mL, "diazoresorcinol" sodium salt solution
(0.1%w/v) 0.50mL, NaHCO33.0g, Na2S·9H2O 0.50g, deionized water 1000.00mL, pH6.8-7.0.Wherein
Trace element solution composition is：Nitrilotriacetic acid 1.5g, MgSO4·7H2O 3.0g, MnSO4·H2O 0.5g, NaCl 1.0g,
FeSO4·7H2O 0.1g, CoCl2·6H2O 0.1g, CaCl20.1g, ZnSO4·7H2O 0.1g, CuSO4·5H2O 0.01g,
AlK(SO4)2.12H2O 0.01g, H3BO30.01g, Na4MoO4·2H2O 0.01g, deionized water 1000mL.
2) seed liquor preparation：The strain of activation is inoculated in 500mL aforesaid liquid culture medium, carries out one-level, two grades of expansions
Big culture；Condition of culture is 80 DEG C.As culture fluid OD600Stop culture when 1.8, obtain first order seed；First order seed is pressed volume
It is seeded in 2L shaking flask and carries out two grades of amplification culture than 10%, stop culture when culture fluid OD600 is 2.5, obtain secondary seed
Liquid.
3) solid fermentation：It powder of straw, municipal sludge, wheat bran and bean cake is by mass percentage 25: 20: 8: 47 ratio
Uniformly mix homogeneously, adds 5% aforesaid liquid culture medium, adjusts 7.0,121 DEG C of sterilizings of pH and makes solid-state fermentation culture medium.
After amplification culture terminates, secondary seed solution is accessed solid-state fermentation culture medium by the inoculum concentration by 10%, mixes, 70 DEG C of culture 10d.
After culture terminates, pulverize and sieve, obtain final product sludge composting heavy metal passivation microbial inoculum.
Embodiment 2
A kind of sludge composting heavy metal is passivated the production method of microbial inoculum, comprises the following steps that：
1) actication of culture：Thermodesulfobacterium commune YSRA-1VC-16 strain is taken to be inoculated in
In 100mL sulfate reducting bacteria liquid-liquid culture medium, cultivate 10d under the conditions of 70 DEG C.Fluid medium is with embodiment 1.
2) seed liquor preparation：The strain of activation is inoculated in 500mL aforesaid liquid culture medium, carries out one-level, two grades of expansions
Big culture；Condition of culture is 70 DEG C.As culture fluid OD600Stop culture when 2.0, obtain first order seed；First order seed is pressed volume
It is seeded in 2L shaking flask and carries out two grades of amplification culture than 10%, stop culture when culture fluid OD600 is 2.8, obtain secondary seed
Liquid.
3) solid fermentation：By powder of straw, municipal sludge, wheat bran and bean cake according to mass percent be 20: 30: 5: 45 ratio
Example uniformly mix homogeneously, adds 5% aforesaid liquid culture medium, adjusts 7.0,121 DEG C of sterilizings of pH and makes solid state fermentation culture
Base.After amplification culture terminates, secondary seed solution is accessed solid-state fermentation culture medium by the inoculum concentration by 10%, mixes, 70 DEG C of cultures
10d.After culture terminates, pulverize and sieve, obtain final product sludge composting heavy metal passivation microbial inoculum.
Embodiment 3
Sludge composting heavy metal is passivated the application examples one of microbial inoculum, comprises the following steps that：
1) compost material pretreatment：Composting material is 30% straw group by the municipal sludge of moisture content 75% and moisture content
Become, mud and straw weight ratio for 5: 2, the moisture content of material about 62% after mixing, adjusting pH value with NaOH is 7.0.
2) microbial inoculum inoculation：Inoculate, in 5% ratio, the heavy metal passivation microbial inoculum that embodiment 1 produces, mix.
3) high temperature aerobic composting：Mixture is carried out aerobic compost, after sludge composting temperature reaches 60 DEG C, daily turning
Once；Fermentation 30d.
4) crush and screen：Treat that mud heap temperature is down to less than 35 DEG C and is pulverized sludge composting, sieve.
5) heavy metal passivation effect inspection：Sludge composting heap, to after the completion of make, detects Cu, Cr heavy metal valency in sludge composting
State and Cu, Pb, Cr, Cd isoreactivity state content of beary metal.Result shows, and compares, and Cu (II), Cr (VI) content reduce
75.89% and 82.18%, Cu, Pb, Cr, Cd activated state content of beary metal declines 76.98,85.64,87.28 and 86.45 respectively,
Illustrate to add the in-situ passivation that this heavy metal deactivator is capable of Cu, Pb, Cr, Cd heavy metal during compost composting.
Embodiment 4
Sludge composting heavy metal is passivated the application examples two of microbial inoculum, comprises the following steps that：
1) compost material pretreatment：Composting material is by the municipal sludge of moisture content 65% and wood flour group that moisture content is 40%
Become, mud and wood flour weight ratio for 5: 1, material water ratio about 61% after mixing, adjusting pH value with NaOH is 7.5.
2) microbial inoculum inoculation：Inoculate, in 2% ratio, the heavy metal passivation microbial inoculum that embodiment 2 produces, mix.
3) high temperature aerobic composting：Mixture is carried out aerobic compost, after sludge composting temperature reaches 50 DEG C, daily turning
Once；Fermentation 25d.
4) crush and screen：Treat that mud heap temperature is down to less than 35 DEG C and is pulverized sludge composting, sieve.
5) heavy metal passivation effect inspection：：After the completion of mud composting, detection sludge composting in Cu, Cr heavy metal valence state and
Cu, Pb, Cr, Cd isoreactivity state content of beary metal.Result shows, and compares, and Cu (II), Cr (VI) content reduce
70.85% and 80.24%, Cu, Pb, Cr, Cd activated state content of beary metal declines 68-85%, illustrates during compost composting
Add the in-situ passivation that this heavy metal deactivator is capable of Cu, Pb, Cr, Cd heavy metal.
Claims (3)
1. a kind of sludge composting heavy metal is passivated microbial inoculum, by sulphate-reducing thermophilic bacterium Archaeoglobus veneficus
VC-16 (DSM 4304), Thermodesulfobacterium commune YSRA-1 (DSM 2178) one or two
Ferment is obtained, and including sulphate-reducing thermophilic bacterium and adjuvant, it is characterized in that：The sludge composting heavy metal passivation every g of microbial inoculum contains thermophilic sulfur
Hydrochlorate reducing bacteria more than 500,000,000, described adjuvant includes powder of straw, municipal sludge, wheat bran and bean cake：Powder of straw weight than 20-30 part,
Municipal sludge weight compares 1-5 than 5-10 part, bean cake weight than 30-50 part, fluid medium weight than 20-30 part, wheat bran weight
Part.
2. a kind of sludge composting heavy metal is passivated the production method of microbial inoculum, it is characterized in that：Step is as follows：
1) actication of culture：Take sulfate reducting bacteria strain to be inoculated in culture medium, cultivate 5-10d under the conditions of 45-85 DEG C；Preferably
, Archaeoglobus veneficus VC-16 cultivation temperature is 80 DEG C, Thermodesulfobacterium commune
YSRA-1 cultivation temperature is 70 DEG C；
2) seed liquor preparation：The strain of activation is inoculated in fluid medium, carries out one-level, two grades, three-level amplification culture；Training
Foster condition is 45-80 DEG C；Stop culture when culture fluid OD600 is between 1.8-3.0, obtain first order seed；First order seed is pressed
Volume ratio 10% is seeded in the shaking flask of more than 1L and carries out two grades of amplification culture, stops when culture fluid OD600 is between 1.8-3.0
Only cultivate, obtain secondary seed solution；
3) solid fermentation：After amplification culture terminates, by the inoculum concentration of 5-10%, secondary seed solution seed liquor is accessed solid fermentation
Culture medium, 45-85 DEG C of fermentation culture 5-10d；After culture terminates, pulverize and sieve, obtain final product sludge composting heavy metal passivation microbial inoculum；
Wherein, step 1) and step 2) described liquid culture based component is：KCl 0.34g, MgCl2·6H2O 4.00g,
MgSO4·7H2O 3.45g, NH4Cl 0.25g, CaCl2·2H2O 0.14g, K2HPO40.14g, NaCl 18.00g, yeast extract
0.50g, sodium lactate 1.50g, Fe (NH4)2(SO4)2·7H2O solution (0.1%w/v) 2.00mL, trace element solution
10.00mL, "diazoresorcinol" sodium salt solution (0.1%w/v) 0.50mL, NaHCO33.00g, Na2S·9H2O 0.50g, deionized water
1000.00mL, pH6.8-7.0；Described trace element solution composition is：Trace element solution composition is：Nitrilotriacetic acid 1.5g,
MgSO4·7H2O 3.0g, MnSO4·H2O 0.5g, NaCl 1.0g, FeSO4·7H2O 0.1g, CoCl2·6H2O0.1g,
CaCl20.1g, ZnSO4·7H2O 0.1g, CuSO4·5H2O 0.01g, AlK (SO4)2·12H2O 0.01g, H3BO30.01g,
Na2MoO4·2H2O 0.01g, deionized water 1000mL；
Described sulfate reducting bacteria is Archaeoglobus veneficus VC-16, Thermodesulfobacterium
CommuneYSRA-1 one or two；
Step 3) described solid-state fermentation culture medium preparation method is：By powder of straw, municipal sludge, wheat bran and bean cake high speed pulverization
Machine is pulverized, and crosses 60 mesh sieves, according to mass percent for 20-30: 20-30: 5-10: 30-50 ratio uniform mix homogeneously, adds
The fluid medium of 1-5%, 121 DEG C of sterilizings；Described fluid medium be step 1) in fluid medium consistent.
3. sludge composting heavy metal described in claim 1 is passivated the application of microbial inoculum.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107470351A (en) * | 2017-09-29 | 2017-12-15 | 吴洪生 | A kind of Eco-friendly original position quick bio repairing heavy metal in soil pollution method and application |
| CN107971336A (en) * | 2017-12-11 | 2018-05-01 | 西南科技大学 | A kind of Lead Pollution in Soil biology in situ renovation method |
| CN111187103A (en) * | 2020-03-16 | 2020-05-22 | 山西宁邦生物科技有限公司 | Sludge compost heavy metal passivation microbial inoculum, and production method and application thereof |
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| CN107971336A (en) * | 2017-12-11 | 2018-05-01 | 西南科技大学 | A kind of Lead Pollution in Soil biology in situ renovation method |
| CN111187103A (en) * | 2020-03-16 | 2020-05-22 | 山西宁邦生物科技有限公司 | Sludge compost heavy metal passivation microbial inoculum, and production method and application thereof |
| CN111187103B (en) * | 2020-03-16 | 2022-11-29 | 山西宁邦生物科技有限公司 | Sludge compost heavy metal passivation microbial inoculum, and production method and application thereof |
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