CN1114983A - Yeast fixed carrier and preparation method - Google Patents
Yeast fixed carrier and preparation method Download PDFInfo
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- CN1114983A CN1114983A CN95114895A CN95114895A CN1114983A CN 1114983 A CN1114983 A CN 1114983A CN 95114895 A CN95114895 A CN 95114895A CN 95114895 A CN95114895 A CN 95114895A CN 1114983 A CN1114983 A CN 1114983A
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- yeast
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- spongy
- sodium alginate
- calcium
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a yeast carrier and a process for preparing the yeast carrier during ferment process. In the existing technology, the yeast carrier for ferment uses an embedding method and an adsorption method with a plurality of shortcomings, such as complex preparing process and short service time of the carrier, etc. The present invention combines the embedding method and the adsorption method organically. The present invention is characterized in that spongy structure materials or fibrous structure materials adsorbing the yeast are dipped in the calcium chloride solution, thus changing sodium alginate gels to calcium alginate gels. Yeast cells are formed in a combined fixing way with two kinds of materials which are the calcium alginate gels and the spongy structure materials or the fibrous structure materials. Comparing with the existing technology, the carrier of the present invention has the advantages of excellent mechanical strength, long service time, high activity and simple preparing process.
Description
A kind of yeast vector and manufacture method that relates to zymotechnique.In alcohol and beer production fermenting process, using fixed yeast is the emerging biotechnology that this century, the seventies was grown up by enzyme immobilization technology.Since the cell density that fixed yeast can make fermentation system at double in addition tens of times of ground increase, and be suitable for operating under the highly diluted rate, thereby can increase substantially fermenting speed, shorten fermentation period, improve equipment capacity and production efficiency, simplifying production process reduces labor intensity, make production process more stable and be easy to control, because above-mentioned advantage, immobilized yeast technology has become the important development direction of alcohol and beer industry fermentation technique, is domestic and international at present very active research field.Immobilized yeast technology roughly can be divided into absorption method and entrapping method two big classes, and the absorption method method is simple, and is less demanding to reactor, the solid support material physical strength is higher, and the life-span is longer, thereby cost is lower, but shortcoming is that the cell concentration of absorption is very low, and when the environmental change of mash (as high flow rate, CO
2It is fierce to discharge, and it is violent to roll, when mash PH changes) cell that is adsorbed comes off easily; cause active decline, and easily the assorted bacterium of absorption is difficult to remove, the advantage of entrapping method is that cell is subjected to the carrier protection to be difficult for running off; stronger to the severe environment resist ability; can carry out specialization and produce, but its shortcoming is complexity of making processes that carrier life is not long; price is higher; and carrier is easily lost physical strength, blocking pipeline, and cost is improved.In recent ten years, calcium alginate embedded method is owing to have condition of cure gentleness easy to make, stronger to reactor adaptability, be suitable for advantages such as multiplication culture, and be much accounted of use, the weakness that investigators exist according to it has again been invented the proliferative cell method and has been added the method that certain material improves the gel physico-chemical property, and the proliferative cell method is to make CO by the starting point concentration that reduces embedding cell
2The release mitigation that becomes, thereby delay the gel phenomenon of bursting apart, but because the starting point concentration of embedding cell is restricted, and phosphatic stable to gel, obviously do not improve, improve the method for gel physico-chemical property for adding subsidiary material, can make original calcium alginate embedded method obtain very much progress, but all these methods can't fundamentally solve the subject matter that alginate calcium exists.
The present invention puts forward in order to address the above problem just, it is not from the physico-chemical property of gel aspect own, directly overcome the weakness of alginate calcium, but by the way of adsorbing and two kinds of methods of embedding are joined together is solved, thisly use two kinds of methods to form unified carrier, unite the technology of fixing pure parent cell, introduce new notion for the immobilized cell field in same process, solve a difficult problem for a long time, make the engineering of immobilized cell be able to widespread use.
Fermenting process provided by the invention (being mainly used in alcohol and beer fermentation process) yeast fixation support, be a kind of equally distributed the spongy of yeast gel or filamentary structure material of containing that adsorbed, yeast cell wherein is to limit fixing simultaneously by two kinds of materials.To be yeast cell united fixing by calcium alginate gel and spongy or filamentary structure material and form, and this during the fermentation associating fixed yeast can be used as the provenance of free yeast simultaneously both as the fermentation activity main body.Spongy or filamentary structure material can be open cell expanded rubber and latex foam with open cells or chemical fibre material among the immobilization material of carrier, as polyethylene fibre, thermovyl, polypropylene fibre, the polyacrylonitrile series fiber, PVAL, polyester fiber, tynex, spandex fiber, fluorofibre, trivinyl fthre, sex change polyacrylonitrile etc., the manufacture method of said fixing carrier is to draw with spongy or fibrous material to contain zymic alginate calcium solution, this material is immersed in the calcium chloride solution at least 24 hours, sodium alginate exchange becoming calcium alginate gel, yeast cell is promptly finished the associating immobilization process along with gelation process is fixed in the calcium chloride solution equably.The concentration of sodium alginate soln is 1--3% (W/V), and optimum value is that 2% (W/V) yeast cell content is 10
6-10
8Individual/milliliter, optimum value is 10
8Individual/milliliter, the concentration of calcium chloride solution is 2-10% (W/V), and the consumption that this carrier is used for fermenting process is that zymamsis is 0.5-5% (V/V), and the highest consumption is 30% (V/V).The beer fermentation consumption is 5--15% (V/V), is up to 30% (V/V).
The concrete operations step of solidification process is: use through the spongy of sterilising treatment or the absorption earlier of fibrous carrier material to contain the zymic sodium alginate soln, adhesion ground, a piece ground of solid support material is dropped in the calcium chloride solution, make sodium alginate in carrier, be exchanged into calcium alginate gel, yeast cell is fixed in the unified carrier equably with gelation process, in calcium chloride solution, solid support material soaks at least can be finished gelation process in 24 hours and soak and to finish with aseptic clear water cleaning 3 times, can drop into and carry out multiplication culture in the fermentor tank, also can directly drop into the fermentation stage running, the condition control and the fixed yeast fermentation technique basically identical commonly used of fermenting process.
The present invention's characteristics compared with prior art have three aspects:
At first in the process for fixation operating process, prior art is that sodium alginate dissolving back mixes with the yeast cell suspension, drops into then to splash into together after certain subsidiary material (as sand grains, polyvinyl chloride fragment, silicon grain etc.) make it evenly or inject calcium chloride solution.Change gelation, these methods are to be operating main body with the sodium alginate soln, promptly operate and be treated to the master with liquid phase, other material is that complementary mixing is entered, to improve the gel physico-chemical property, operating process of the present invention is with above-mentioned opposite, be to be operating main body with spongy or fibrous carrier piece, promptly be operating as the master with solid phase, sodium alginate soln is that passive quilt is drawn, main if it were not for in sodium alginate and the carrier impouring calcium chloride solution together, exchange gelation but have piece ground of carrier of sodium alginate soln to drop in the calcium chloride solution suction, gel be limited in spongy or the fibrous carrier structure in, yeast cell then is fixed with gelation, and the gel sets cell makes it can not free movement, and spongy or fibrous carrier structure within it can not dissipation the gel restriction, in fact yeast cell is limited simultaneously by a kind of embedded material and two kinds of materials of a kind of sorbing material and is these two kinds of materials of associating and consists of unified carrier, is the joint vector immobilization.Secondly, the mode of keeping in fixed yeast type of service of the present invention and work-ing life is only with relevant as the fibrous of sorbing material or spongy material quality performance, and it is little with alginate calcium relation as embedded material, generally can use more than 4 years continuously, effect is not fallen, and simple calcium alginate gel embedding of the prior art, its carrier is difficult to above three months work-ing life.The 3rd characteristics of the present invention are: existing other yeast process for fixation is simple adsorption form or simple embedding form, and the present invention adopts the form of joint vector to gather the two length of absorption and embedding, organically combine, the advantage that calcium alginate gel is made embedded material is the rigid condition gentleness, nontoxic, outside cell is difficult to enter in the gel.In the joint vector form, calcium alginate gel only plays thalline embedding fixed action and spongy and fibrous object is born the profile support, and keeping of physical strength and work-ing life in use, can be avoided the deadly defect of calcium alginate gel, promptly because of CO
2Discharge fierceness and disintegration; with lose physical strength because of the phosphoric acid salt unstable; calcium alginate gel is subjected to the protection and the support of sponge block or fibrous material; even burst apart with lose physical strength and still can be adsorbed effectively to carry and stay spongy or fibrous object relaying supervention is waved effectiveness; can avoid the weak shortcoming of the not high anti-assorted bacterium ability of adsorption rate of simple absorption method; can avoid the simple short shortcoming of gel embedding method carrier life again, and show exclusive advantage.In addition, the carrier that the present invention relates to is adjourned living contaminants in fermentation, mechanical fault, or water power is obstructed more more convenient than prior art to work such as carrier cleaning removal of impurities bacterium and activation.
The present invention's outstanding effect compared with prior art is at 4 * 7.5M
3The big production plant test manufacture of 24 tons of alcohol is tested and produced daily to the test plant of scale, and the result can embody contrast and be expressed as follows:
4 * 7.5M
3The test plant test-results:
| Carrier immobilized form | ||
| Jar fermenter cubic capacity M 3 | The associating immobilization | The gel beads embedding |
| ??4×7.5 | ??4×7.5 | |
| Carrier injected volume %V/V | ??8 | ??30 |
| Fermentation time br | ??12 | ??8 |
| Alcohol production, can make every effort to overcome ethanol/rise cylinder hour gram ethanol/liter, carrier hour | ??6.21 ??62.1 | ??8.65 ??21.5 |
| Ripe raw spirit part %V/V | ??9.44 | ??8.6 |
| Transformation efficiency % | ??93.7 | ??90.2 |
| Stream adds form | Single dense, batch-continuously | Single dense, batch-continuously |
| Days of operation (my god) | ??120 | ??15 |
| Solidify pure female input cost (unit) | ??5690 | ??24740 |
The result shows, simple gel embedding uses that activity has begun to descend after 15 days, still can keep activity not fall and unite to solidify to use after 120 days.
Produce 24 tons of big production plant test manufacture results of alcohol such as following table daily:
| The associating immobilized enzyme | Conventional free yeast | |
| Fermentor tank cubic capacity (M 3 | ?????500 | ??500 |
| Carrier injected volume %V/V | ?????0.4 | ???--- |
| Fermentation time (hour) | ???32--34 | ??46--48 |
| Stream adds form | Two dense continuous | Two dense continuous |
| Average daily production (ton alcohol) | ????24.68 | ??21.27 |
| Full phase output (ton alcohol) | ????3136 | ??2702 |
| Days of operation (my god) | ????150 | ??150 |
| Raw material consumption ton sugar is close/ton alcohol | ????4.023 | ??4.198 |
The result shows, though associating fixed yeast injected volume only be 0.4% can improve throughput, 16% shortening time 30%, reduce the complete according to statistics ripe raw spirit part of phase of raw material consumption simultaneously and on average improved 0.48% (V/V), plant factor improves 30--35%, increases by 1,500,000 yuan of the output values, increases by 540,000 yuan of taxes and profits, and the cost input of associating solidified yeast only is 70,000 yuan, and benefit is very remarkable.
Embodiment 1
The close quick production alcohol of the associating fixed yeast fermentation rare sugar of sugarcane.
One, material:
1) bacterial classification:
The AS2.1190 yeast strain is supplied by Institute of Micro-biology of the Chinese Academy of Sciences.
Sodium alginate, Xiamen, Fujian refrigeration plant production food grade.
2) sponge: 30 * 30 * 30 squares
3) calcium chloride: chemical pure
4) oleic acid: chemical pure
5) tween 80: chemical pure
6) substratum: 1) test tube slant solid medium, the bent juice substratum of rice commonly used
2) triangular flask liquid nutrient medium: Yeast diffusion juice 0.3%, urea 0.3%
Sucrose 10%, potassium primary phosphate 0.2%, magnesiumcarbonate 0.051%, PE:
4.5--5.0 regulate 1 kilogram/cm of sterilization with sulfuric acid
330 minutes.
3) increment liquid nutrient medium triangular flask liquid nutrient medium
4) the rare sugar of fermentation is close: 55B power acidifying 6 hours, dilution 24--26B power contains
Sugar about 17% (common cane sugar factory is paid product) potassium primary phosphate
0.5%, urea 0.025%, sal epsom 0.02%.
7) fermentation reactor: 120 * 450 specimen bottles, interior dress stainless steel intercepts net (be used for carrier is limited in below the karusen liquid level, guarantee its effect)
8) plant and instrument microbiological culture media commonly used and chemical analysis instrument equipment have: electric furnace, constant incubator, shaking table, microscope etc.
Two, method
(1) preparation of mother liquor is solidified in embedding:
Bacterium liquid is cultivated: get one of the female kind in test tube slant, 30 ℃ of constant temperature culture of a collection of test tube slant production of tube kind 24 hours, get and cultivated one of test tube production kind, 30 ℃ of rotational oscillations cultivations of 500 milliliters of triangular flasks (including the 300ml liquid culture) (rotating speed is 60--80 rev/min) are gone in inoculation, and promptly maturation was standby in about 24 hours.And the yeast growth situation of taking a sample to check, require to contain hundred million/milliliter of yeast 1.2--1.5, bud ratio 20--30%, no living contaminants can use.
Mother liquor preparation: take by weighing sodium alginate 20 grams, after 500 milliliters of dissolvings fully of sterilized water, add bacterium liquid 250ml, 10 milliliters, oleic acid and 5 milliliters, tween 80, after stirring, add sterilized water to 1000 milliliters of volumes, stir embedding solidify mother liquor, the working concentration of various materials is: sodium alginate 2%, yeast suspension 25%
Tween 80 0.5%
Oleic acid 1.0%
(2) associating immobilized yeast
The preparation of exchange liquid: take by weighing calcium chloride 200 grams,, be settled to 2000 milliliters, be made into 10% concentration calcium chloride solution with the sterilized water dissolving.
The preparation of sponge block: get 30 * 30 * 30mm
320 of the sponge blocks of/block specifications clean 3 times with tap water, dehydrate back 105 ℃ of following steam sterilizings 30 minutes, are cooled to room temperature under the aseptic condition and slough residual moisture standby.
Associating immobilization: under aseptic technique, with the sponge block of handling well, draw the embedding mother liquor one by one to state of saturation, careful then the input in the exchange liquid, sponge block is dipped under the liquid level, all behind the sponge block end of operation, after continuing to soak about 24 hours in exchange liquid, the calcium alginate gel in the sponge, yeast cell are subjected to the double fixed of gel and sponge block, after the immersion exchange finishes, take out sponge block, use sterile water wash 3 times, promptly can be used for fermenting process, it is good that also available 2% calcium chloride solution, normal temperature are preserved a month activity down.Active carrier soaks normal temperature by 1: 1 volume physiological saline to be preserved 15 days down, preserved two months to half a year down for 4 ℃.
The associating fixed yeast is applicable to various reactors and different processing requirements, carries out zymotechnique.
Embodiment 2
12 ° of worts of associating fixed yeast fermentation are produced beer fast.
One, material
1) bacterial classification: the M1G2.101 yeast strain, Guangdong Microbes Inst DSMZ preserves.
2) sodium alginate: the Qingdao City produces, food grade.
3) sprunstron ball: specification is 25,1 grams/special a manufacturing.
4) calcium chloride: chemical plant, Taishan City, Guangdong produces, chemical pure.
5) substratum: inclined-plane solid medium: malt extract medium commonly used
Triangular flask liquid nutrient medium: wheat juice substratum
Fermentation: contain proliferated culture medium, the hopping of 12 ° of wheat juice is provided by brew-house.
Preceding ferment reactor:
1) preceding ferment reactor: the 3000ml triangular flask, in establish stainless steel restriction net in order to the restriction carrier below liquid level, be advisable near a bottle end with carrier, with cotton yarn beyond the Great Wall tinning paper seal.
2) back ferment reactor: the 2000ml filter flask is a plurality of, in establish stainless steel restriction net, the bottleneck sealing, the suction filtration mouth of pipe keeps a malleation in the bottle with fluid column.
Two, method: the preparation of associating fixed yeast
(1) preparation of embedding mother liquor:
Yeast culture: get a collection of test tube slant production of tube kind of the female kind in test tube slant, 25 ℃ of following constant temperature culture 24 hours, get to cultivate and produce one of good test tube production kind, inoculation is gone into 20 ℃ of rotational oscillations of 500 milliliters of triangular flasks (including 300 milliliters of wheat juice substratum) and cultivated (60--80 rev/min) about 24 hours, and is promptly ripe standby.
The preparation of mother liquor: take by weighing sodium alginate 20 gram, after the dissolving of 50 ml sterile waters, add and cultivate 250 milliliters of sophisticated yeast liquid, fully stir, even embedding curing mother liquor, wherein sodium alginate concentration is 2%, yeast liquid is 25%.
(2) associating immobilized yeast:
The preparation of exchange liquid: take by weighing calcium chloride 100 grams, be settled to 2000 milliliters, make it to become 5% calcium chloride solution with the sterilized water dissolving.
The preparation of chemical fibre ball: get 50 chemical fibre balls, clean with boiling tap water, clean once with distilled water, the back 105 ℃ of sterilizations 60 minutes of dewatering are taken out and are cooled to normal temperature, slough residual moisture content.
Unite fixing:
Under strict aseptic technique, the chemical fibre ball of handling well is drawn mother liquor singly, to state of saturation, the careful input in the exchange liquid, must make that the chemical fibre ball is whole to be dipped in below the liquid level, after all the chemical fibre ball exercises are done to finish, in exchange liquid, continue to soak 24 hours, make the sodium alginate in the chemical fibre ball all be exchanged into calcium alginate gel, after soaking the exchange end, take out carrier sterile water wash 2 times, soaked 30 minutes with sterilized water again, and repeat twice, make residual Na
+, Ca
+Deng full scale clearance, whole solidification process requires envrionment temperature not to be higher than l5 ℃, and after the associating immobilization was finished, carrier can directly drop into the preservation under 0--2 ℃ of the also available rare wheat juice of fermentation stage and re-use in one to three months.
With the associating immobilized yeast vector that this special chemical fibre ball is made, fermentation, all be better than the performance of sponge carrier from every side.
Embodiment 3
Associating fixed yeast amylum hydrolysate of the sugar is produced alcohol fast
One, material:
1) bacterial classification: MIG2.44 (Nanyang 1308) yeast strain. the Guangdong Microbes Inst supplies
2) sodium alginate: Xiamen, Fujian Province refrigeration plant production food grade
3) thermovyl piece: meet loose shape 150 * 50 * 10mm
3The sheet rectangular parallelepiped is ordered specially and is made
4) calcium chloride: Taishan City Chemical Factory Guangdong Prov. produces, chemical pure
5) oleic acid: tween 8O Guangzhou Chemical Reagent Factory production chemical pure
6) substratum: test tube slant solid medium: rice
The juice nutrient agar,
The triangular flask liquid nutrient medium: yeast extract paste, dextrose culture-medium,
Propagation liquid nutrient medium: with example 2
Fermention medium: the cassava starch converted mash, grain distillery provides, saccharification
1--2 hour, be cooled to the 28--30 ℃ of same conventional method of nutritive salt amount.7) fermentation reactor: 7.51 small-sized fermentation cylinders, in establish carrier and intercept net 8) plant and instrument: microorganism culturing commonly used and chemical analysis instrument equipment two, method: 1) preparation (with embodiment one) of mother liquor is solidified in embedding
2) associating immobilized yeast:
The preparation of exchange liquid: (with example one)
The preparation of fiber block: get 10 of fiber blocks, clean up with tap water, after the dehydration, 105 ℃ of following steam sterilizings 30 minutes under the aseptic condition, are cooled to room temperature and slough residual moisture, and are standby.
Associating immobilization: under aseptic technique, ready fiber block is drawn the embedding mother liquor one by one, careful then the input in the exchange liquid, must make fiber block all be dipped under the liquid level, all behind the fiber block end of operation, in exchange liquid, continue to soak 24 hours, sodium alginate in the fiber block all is exchanged into calcium alginate gel, wherein yeast cell is subjected to gel and fiber block structured double fixed, after the immersion exchange finishes, take out sponge block sterile water wash 3 times, promptly can be used for soaking under the also available 2% calcium chloride solution normal temperature of fermenting process, preserve a month activity well.
This associating immobilized yeast vector can be used in the zymotechnique.
Claims (8)
1, a kind of yeast fixation support that is used for fermenting process, this carrier is a kind of resilient solid material, it is characterized in that adsorbing the equally distributed material that contains the spongy or filamentary structure of yeast sodium alginate gel, wherein contained yeast cell is to be limited fixingly simultaneously by two kinds of materials, and promptly yeast cell is united fixing and form by calcium alginate gel and spongy or filamentary structure material.
2, according to the carrier described in the claim 1, it is characterized in that the immobilization material sponge or the filamentary structure material of carrier, can be open cell expanded rubber or latex foam with open cells or man-made fiber class material.
3,, it is characterized in that man-made fiber class material can be: polyethylene fibre, thermovyl, polypropylene fibre, acrylic fiber, PVAL, polyester fiber, tynex, spandex fiber, fluorofibre, trivinyl fthre, modified acryl fibre according to the carrier described in claim 1 and 2.
4,, it is characterized in that described volume spongy or the filamentary structure material can be controlled at 0.5--1000cm according to the carrier described in the claim 1
3/ piece, only specification are 1.0--100cm
3/ piece.
5, a kind of method of making the carrier described in the claim 1 that is exclusively used in, it is characterized in that spongy or fibrous material has been drawn and contained the zymic sodium alginate soln, material is immersed in the calcium chloride solution at least 24 hours, treat that sodium alginate is exchanged into calcium alginate gel, yeast cell is along with gelation process is fixed in the calcium chloride solution equably, promptly finish the associating immobilization process, form the associating immobilized yeast vector.
6, method according to claim 5, the concentration that it is characterized in that described sodium alginate soln are 1--3% (W/V), and optimum value is 2% (W/V), and wherein yeast cell content is (10
6--10
9Individual/milliliter), optimum value is 10
8Individual/milliliter.
7, method according to claim 5, the concentration that it is characterized in that described calcium chloride solution are 2--10% (W/V), and optimum concn is 10% (W/V).
8, according to the carrier described in claim 1 and 5, it is characterized in that the consumption that this carrier is used for fermenting process is: zymamsis is 0.5--5% (V/V), be up to that (30% (V/V), beer fermentation consumption are 5.0--15 (V/V), are up to 30% (V/V).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95114895A CN1064709C (en) | 1995-04-28 | 1995-04-28 | Yeast fixed carrier and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95114895A CN1064709C (en) | 1995-04-28 | 1995-04-28 | Yeast fixed carrier and preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1114983A true CN1114983A (en) | 1996-01-17 |
| CN1064709C CN1064709C (en) | 2001-04-18 |
Family
ID=5080374
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95114895A Expired - Fee Related CN1064709C (en) | 1995-04-28 | 1995-04-28 | Yeast fixed carrier and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1064709C (en) |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997013850A1 (en) * | 1995-10-06 | 1997-04-17 | Yaodong Wang | Living-yeast immobilization method and application device thereof |
| CN1089806C (en) * | 1996-10-09 | 2002-08-28 | 广东省微生物研究所 | Fixed yeast xerogel bead and prepn. method thereof |
| CN101153294B (en) * | 2007-08-30 | 2010-11-10 | 合肥工业大学 | Immobilized cell single-tank high-strength continuous fermentation process for succinic acid |
| CN102491528A (en) * | 2011-11-16 | 2012-06-13 | 温州大学 | Filler device for sewage treatment and manufacturing method of porous active filler block in the filler device |
| CN102517277A (en) * | 2012-01-12 | 2012-06-27 | 南京林业大学 | Enzyme immobilization technology by film forming through carrier surface phase transformation method |
| WO2014089811A1 (en) * | 2012-12-13 | 2014-06-19 | 南京工业大学 | Preparation method of yeast cell immobilization medium and application thereof |
| CN105255649A (en) * | 2015-11-16 | 2016-01-20 | 湖北工业大学 | Immobilized and mechanized fermentation method for increasing concentration of white spirit liquid fermented fragrance ingredients |
| CN108360088A (en) * | 2018-02-28 | 2018-08-03 | 清华大学深圳研究生院 | The method and apparatus for preparing calcium alginate fibre |
| CN108396024A (en) * | 2018-05-21 | 2018-08-14 | 郑州大学 | A kind of micro-embedded pseudomonas putida composite biological packing and preparation method |
| CN111593044A (en) * | 2019-02-20 | 2020-08-28 | 成都医学院 | Rhodotorula mucilaginosa immobilized cell and application thereof |
| CN112812912A (en) * | 2021-04-01 | 2021-05-18 | 浙江大学 | Household self-separation beer fermentation barrel and fermentation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3410650A1 (en) * | 1984-03-23 | 1985-10-03 | Kernforschungsanlage Jülich GmbH, 5170 Jülich | POROISE INORGANIC CARRIERS GROWN WITH MICRO-ORGANISMS, METHOD FOR IMMOBILIZING MICRO-ORGANISMS AND CARRIER BODIES SUITABLE FOR THIS |
-
1995
- 1995-04-28 CN CN95114895A patent/CN1064709C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997013850A1 (en) * | 1995-10-06 | 1997-04-17 | Yaodong Wang | Living-yeast immobilization method and application device thereof |
| CN1089806C (en) * | 1996-10-09 | 2002-08-28 | 广东省微生物研究所 | Fixed yeast xerogel bead and prepn. method thereof |
| CN101153294B (en) * | 2007-08-30 | 2010-11-10 | 合肥工业大学 | Immobilized cell single-tank high-strength continuous fermentation process for succinic acid |
| CN102491528A (en) * | 2011-11-16 | 2012-06-13 | 温州大学 | Filler device for sewage treatment and manufacturing method of porous active filler block in the filler device |
| CN102517277A (en) * | 2012-01-12 | 2012-06-27 | 南京林业大学 | Enzyme immobilization technology by film forming through carrier surface phase transformation method |
| WO2014089811A1 (en) * | 2012-12-13 | 2014-06-19 | 南京工业大学 | Preparation method of yeast cell immobilization medium and application thereof |
| CN105255649A (en) * | 2015-11-16 | 2016-01-20 | 湖北工业大学 | Immobilized and mechanized fermentation method for increasing concentration of white spirit liquid fermented fragrance ingredients |
| CN108360088A (en) * | 2018-02-28 | 2018-08-03 | 清华大学深圳研究生院 | The method and apparatus for preparing calcium alginate fibre |
| CN108396024A (en) * | 2018-05-21 | 2018-08-14 | 郑州大学 | A kind of micro-embedded pseudomonas putida composite biological packing and preparation method |
| CN111593044A (en) * | 2019-02-20 | 2020-08-28 | 成都医学院 | Rhodotorula mucilaginosa immobilized cell and application thereof |
| CN112812912A (en) * | 2021-04-01 | 2021-05-18 | 浙江大学 | Household self-separation beer fermentation barrel and fermentation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1064709C (en) | 2001-04-18 |
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