CN1155010A - Fixed yeast xerogel bead and prepn. method thereof - Google Patents
Fixed yeast xerogel bead and prepn. method thereof Download PDFInfo
- Publication number
- CN1155010A CN1155010A CN 96119159 CN96119159A CN1155010A CN 1155010 A CN1155010 A CN 1155010A CN 96119159 CN96119159 CN 96119159 CN 96119159 A CN96119159 A CN 96119159A CN 1155010 A CN1155010 A CN 1155010A
- Authority
- CN
- China
- Prior art keywords
- pearl
- xerogel
- calcium
- liquid
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The present invention relates to a fermentation technllogy of alcohol and beer production, which uses a separation forming process under liquid level to prepare dried yeast gel granules. Under the liquid level of calcium chloride solution a nozzle tube is placed, and the mixed mother liquor is ejected from the nozzle tube and formed. The diameter of this wet gel granule can be up to 30 mm, its dried diameter can be up to 18 mm, and the compressive strength of single granule can be up to 30-100 kg/granule. Said invented dried gel granule is large in grain size, good in activity, and is applicable to covnentional fermentation equipment.
Description
The invention belongs to microorganism cells immobilization technology field and relate to the fermentation technique of alcohol and beer.
The immobilization technology of the microorganism whole cell that this century, the seventies grew up is an emerging biotechnology, and immobilized yeast technology is mainly used in the fermenting process of alcohol and beer, the application during the fermentation of this technology can be quickened fermentation, increase output, and make stable operation, be convenient to control, thereby be one of developing direction of alcohol and beer industry fermentation technique, in the prior art, the most frequently used immobilized yeast technology is the calcium alginate gel beads entrapping method, (1989 the 1st phases Gansu science journal, " research that the fixed hyperplasia yeast industry is used "), its ultimate principle and operation are that the sodium alginate aqueous solution that will contain yeast cell drop by drop splashes in the calcium chloride solution, make the sodium ion in the sodium alginate be exchanged into calcium ion, harden for some time at a certain temperature, just become insoluble aquation gel beads.This gel beads requires to carry out fermentation reaction on special reactor, because the very little general diameter of the granularity of gel beads is all below 4mm, this gel beads is in big production, because bead is in heaps, mutual gap is too small, influenced the contact reacts of mash and carrier, therefore also can hold back foreign material such as scum silica frost in the mash and silt, these foreign material are hotbeds that assorted bacterium grows, the living contaminants chance is increased, make carrier with alginate calcium on the other hand, also can have two big weakness, the one, to phosphatic instability, the 2nd, because inner CO
2Discharge fierce and growing microorganism and gel structure is burst apart and expand loose, this two big weakness all can cause gel beads forfeiture physical strength, thereby make carrier life be difficult to prolong, cost increases, and can cause problems such as equipment obstruction, tiny in addition bead is in the big production fermentation operation process of daring not accept as clean, removal of impurities, ventilate, the processing that fiercenesses such as activation are rolled, and between the bead, bead and fermentor tank, particularly and the friction between intercepting, situations such as the extruding during emptying, these have all quickened weakening and the carrier gel loss of bead strength, make carrier life very briefer.
The objective of the invention is in order to overcome the existing in prior technology shortcoming, adopt unique liquid to separate down and make the xerogel pearl by drying treatment again after the moulding method is made the calcium-alginate-immobilized yeast wet gel of volume particle size pearl.Because problems such as this xerogel bead degree is big and Jie Gou Cause is close and hard, can solve two big weakness of and alginate calcium carrier existence too small owing to micelle, and the equipment that causes stops up, and pollutes increase, and bead strength is weakened, and gel disintegrates, and carrier life is very brief.
The gel beads that method of the present invention is prepared the has been a kind of embedding alginate calcium volume particle size xerogel pearl of yeast viable cell is characterized in gel beads by calcium carbonate-filled, and Zhi Di Cause is close and hard, and technical indicator is:
Dry matter content 83-94% (W/W)
Amount of yeast 6-80 hundred million/ml
Simple grain volume 0.07-3.0 (cm)
3
Equivalent diameter 5-18mm
Proportion 1.2-1.65
Simple grain ultimate compression strength 30-100kg/ grain
The method that the present invention prepares the xerogel pearl is: be made into earlier and contain the zymic sodium alginate soln, this strength of solution is 2-4%, be generally 3% (W/V), yeast wet cell content is 7-105g/L, promptly about 1-15 hundred million/ml, optimum value is 5-6 hundred million/ml, and adding lime carbonate does to fill Liu in sodium alginate soln, add-on is that 3 one 12% (W/V) optimum values are 8%, stir, become heavy-gravity mixing mother liquor, preparation method's characteristics are generally can make 15mm by the liquid compacted under method system pearl jet pipe that promptly under the calcium chloride solution liquid level bore to be set be 8-32mm, order mixes mother liquor and sprays from jet pipe, enter in the calcium chloride exchange liquid, the interface of ejection part exchanges immediately and forms one deck calcium alginate gel film, like this, the ejection part just becomes the protruding capsule by film wrapped, treat that the ejection part reaches required bead length, when being generally 15-20mm, in time the protruding capsule of film is squeezed off separation, be shaped to the bead liquid capsule of volume particle size, liquid capsule bead soaked 24 hours under 4 ℃ in exchange liquid again, just it is neat that abundant exchange hardens into profile, the reinforced gel beads of even-grained volume particle size fixed yeast, handle through the cryodrying below 35 ℃ again, it is dry to make it evenly shrink, shrink until its moisture content and granularity and all to reach comparatively steady state, a large amount of yeast viable cell of just having made a kind of embedding and the volume particle size marine alga ferment calcium xerogel pearl of being filled by a large amount of lime carbonate.
The method for preparing gel beads provided by the present invention, difference from prior art is:
1) mixes mother liquor from being dipped in the nozzle hole ejection below the exchange liquid liquid level, after mixing the mother liquor ejection, be to make the protruding capsule that the interface exchange forms the calcium alginate film parcel earlier, and then with mechanical means it is squeezed off and to be separated into pearl liquid capsule, thereby be not subjected to capillary restriction, the prepared gel granularity that goes out is big, the intensity height, the prepared bead of prior art has only the 2-3mm diameter usually, but the wet gel bead Du Keda Φ 30mm that the present invention prepares, still can reach Φ 18mm after the drying, dried pearl simple grain ultimate compression strength can reach the 30-100kg/ grain.
2) gel beads of method preparation provided by the present invention, Zhi Di Cause is close and hard, and contains a large amount of weighting agents, thereby has stoped the scattering and permeating of substrate to bead inside effectively, makes inner CO
2Release and Yeast proliferation are very slow, avoid the disintegration of bead, because support strength increases, make phosphatic stability reinforcement simultaneously, have avoided the problem of the loose disintegration of carrier fully.
Gel beads technology involved in the present invention, compared with prior art list in the table 1 and can see by table 1 data for the life-span contrast of the effect of fermentation test and carrier, it is big that xerogel pearl of the present invention can reach granularity, the intensity height, active good, the advantage that life-span is long is applicable to use in the conventional fermentation equipment and promotes.
Example 1, calcium-alginate-immobilized yeast xerogel pearl fermentation sugarcane diluted molasses are produced alcohol fast
(1) material:
1, bacterial classification, AS, 21190 bacterial strains, the supply of institute of microbiology of the Chinese Academy of Sciences.
2, light calcium carbonate: chemical plant, the Taishan, Guangdong Province produces.
4, calcium chloride: chemical plant, the Taishan, Guangdong Province produces, chemical pure.
5, substratum: (1) test tube slant solid medium: rice koji agar commonly used.
(2) shake a bottle liquid cell culture medium: yeast leaches 0.7%, urea 0.3%, and sucrose 10%, potassium primary phosphate 0.2%, sal epsom 0.05, PH:4.5-5.0, sulfuric acid is regulated, 1kg/cm
2Sterilization in 30 minutes.
(3) fermention medium: 6 hours cane molasses of 55 acidifyings, be diluted to 24-26Bx, it is about 15% to contain sugar, PH4.0-4.5.
6, liquid separates solidifying and molding device down: one in Φ 120mm funnel with one of the flat mouthful glass-tube of Φ 15mm, couples together with emulsion tube, get one of Φ 250mm glass jar, funnel is fixed on about 400mm place, glass jar top with fixed support, and a flat mouthful glass-tube then is fixed in the glass jar.
7, fermentor tank: polyvinyl chloride system volume is 2 of 1.5 liters of awl end ethanol fermentation tanks, and tapering dress 3-4 order stainless steel intercepts net, when being used for discharging carrier is stayed in the jar.
8, plant and instrument: one of air dry oven and Experiment on Microbiology commonly used and chemical analysis instrument equipment.
(2) method: 1, preparing carriers
(1) gel beads curing molding:
1. mix the preparation of mother liquor: 2 of test tube slant barmses, tap is shaken a bottle liquid nutrient medium for 8 bottles, 30 ℃ of following shaking culture 24 hours, the centrifuging and taking wet cell is standby.Take by weighing sodium alginate 30g, use sterilized water 800ml, the dissolving back adds 56g yeast wet cell, adds the 60g light calcium carbonate after stirring again, is settled to 1000ml with sterilized water then.2. liquid separates moulding system curing system pearl down, take by weighing and be settled to 3000ml after 150g calcium chloride dissolves with sterilized water, 5% the exchange liquid, then it is poured in the glass jar, and will put down a mouthful glass-tube mouth and be dipped under the liquid level, to mix mother liquor again and pour funnel into, make mother liquor flow to flat mouthful glass-tube along emulsion tube, dominant discharge makes it slowly flow in the exchange liquid, whenever to 18mm left and right sides length, squeeze off separation immediately, until whole mixing mother liquor end of operations, 4 ℃ of following stostes were soaked 24 hours, promptly became the volume particle size wet gel pearl of rod-short.
(2) drying of gel beads: with the wet gel pearl that makes, put into sterilized sugared porcelain tray, 35 ℃ of following forced air dryings in air dry oven then, all reach than stable status until its granularity contraction and moisture content, bead becomes hard xerogel structure, its granularity is about Φ 8-8.5mm, can drop into fermentation and use.
2, single dense two jars of batch fermentations: take by weighing 120g xerogel pearl, on average place two 1.5 liter jars respectively, it is the 60g/ jar, and denotation token is respectively 1# and 2# jar, adds the 1200ml fermention medium then in the 1# jar, 32-34 ℃ of fermentation 10 hours, all mash moves in the 2# jar then, 30-32 ℃ is continued about 10 hours of fermentation down, and just fully matured is discharged and passed distillation.After 1# jar mash is all removed, add-back 1200ml fermention medium immediately, 32-34 ℃ of bottom fermentation all moves to the 2# jar again and continues about 10 hours of fermentation after 10 hours, so repetitive operation just had a collection of ripe wine with dregs in then per approximately 10 hours, and its wine branch can reach about 9.4% (V/V), test analysis and Micro biological Tests work and condition control, clean removal of impurities, the activation of ventilating waits operation consistent with ordinary gel pearl technology, is more convenient and easy.Carrier is active can suitably to shorten fermentation time after improving, and when the 2# jar is active when obviously descending, can make 1#, 2# exchanges operation.
Example 2,12 ° of worts of calcium-alginate-immobilized yeast xerogel pearl fermentation are produced beer fast.
(1) material:
1, bacterial classification: MIG2.101 yeast strain: Guangdong Microbes Inst DSMZ supply.
2, sodium alginate: Qingdao City's product, food grade, the supply of Guangdong Province's chemical corp.
3, treated carbonates: chemical plant, the Taishan, Guangdong Province produces.
4, calcium chloride: chemical plant, the Taishan, Guangdong Province produces, chemical pure.
5, substratum: (1) test tube tiltedly and solid medium: the wort agar substratum.
(2) shake a bottle liquid cell culture medium, malt extract medium.
(3) fermention medium: 12 ° of worts, hopping is provided by brew-house.
6, liquid separates curing molding system pearl device down: with example 1.
7, fermentor tank: 5 liter beer fermentation tapered shaped cans, bottom add stainless steel and intercept net, specification 4 orders.
8, Other Instruments equipment: one in refrigerator and other Experiment on Microbiology commonly used and chemical analysis instrument equipment.
(2) method: 1, preparing carriers
(1) gel beads curing molding: carry out under strict aseptic technique, envrionment temperature is below 15 ℃.
1. mix the preparation of mother liquor: test tube tiltedly and 2 of bacterial classifications, 8 bottles of triangular flasks that contain cell culture medium 250ml bottle of tap, 15 ℃ of following shaking culture are more than 24 hours, the centrifuging and taking wet cell, 2-4 ℃ standby down.Take by weighing sodium alginate 35g, after the dissolving of 800ml clear water, sterilized 30 minutes for 105 ℃, coagulate but to 4-10 ℃, add yeast wet cell 28g, stir, add the 87.5g treated carbonates again, evenly the back is settled to 100ml with 4 ℃ of sterilized waters, stir than heavy-gravity mixing mother liquor.
2. liquid separates curing molding system pearl down: take by weighing 150g calcium chloride, with being settled to 3000ml after the sterilized water dissolving, be cooled to 4 ℃ then, promptly become 5% exchange liquid.Thereafter forming operation embodiment one.
(2) drying of gel beads: the wet gel pearl that makes is cleaned 3 times with sterilized water (2-4 ℃), put into refrigerator then, carry out drying under the 0-4 ℃ of aseptic condition,, promptly can be used for fermentation until becoming hard xerogel pearl.
2, single jar batch of worker's fermentation: take by weighing 135g xerogel pearl under the aseptic condition, put into sterilized tapered shaped can, add fermention medium, 8-10 ℃ of bottom fermentation 48 hours, discard whole nutrient solutions, add the 4500ml fermention medium then and carry out a pot type fixed yeast quick fermentation production beer, condition control is all identical with ordinary gel pearl technology with Micro biological Tests etc. with fermentation operation and test analysis, be more convenient and easy and.
Example 3, calcium-alginate-immobilized yeast xerogel pearl fermentation cassava starch hydrolysis sugar are produced alcohol fast.
(1) material
1, bacterial classification, Nanyang mixing yeast strain are supplied by Guangdong Microbes Inst DSMZ.
2, sodium alginate: Qingdao City's product, food grade, the supply of Guangdong Province's chemical corp.
3, light calcium carbonate: chemical plant, the Taishan, Guangdong Province produces
4, oleic acid: the supply of Guangzhou chemical reagent factory, chemical pure.
5, tween 80: the supply of Guangzhou chemical reagent factory, chemical pure.
6, calcium chloride: chemical plant, the Taishan, Guangdong Province produces, chemical pure.
7, substratum: (1) test tube slant solid medium: wort agar substratum.
(2) shake a bottle liquid cell culture medium: malt extract medium.
(3) fermention medium: the cassava starch hydrolysis sugar liquid is provided by starch grain distillery, contains about 15% (W/V) of sugar.
(4) liquid separates curing molding system pearl device down, and with embodiment one, but a flat mouthful glass-tube changes Φ 13mm into.
9, fermentor tank: 3 liter glass awl end ethanol fermentation tank, the bottom adds 4 order stainless steels and intercepts net.
10, plant and instrument is with example one.
(2) method: 1, the preparation of carrier
1, gel beads curing molding:
1. mix the preparation of mother liquor: with example one, just bacterial classification changes mixed yeast into, and in the end adds oleic acid 6g and tween
806g stirs.
2. liquid separates curing molding system pearl down: with example one, just bead liquid capsule length changes 15mm into.
(2) drying of gel beads: with example 1, dried gel beads granularity is about Φ 7mm.
2, single jar of batch fermentation:
Take by weighing 100g xerogel pearl, place 3 liter fermentor tanks, add fermention medium 2.5L, 30-32 ℃ of bottom fermentation, about 36 hours can be ripe, ripe raw spirit part is 9.) about % (V/V), after several batches, the carrier activity reaches to the highest, and about 28 hours can be ripe.The fermenting process operation is all consistent with ordinary gel pearl technology with test analysis and Micro biological Tests work, and is just easier and convenient.
Table 1
| The gel beads technology | The present invention | Part is dry | The proliferative cell method |
| Rehydration or propagation after fermentation are after 10 days after dry after carrier granularity (Φ, the mm) moulding | 15 8 10 10.5 | 2 1.5 2 3 | 3 3 4 |
| Dry matter content (%, W/W) | 92 | 60 | 10 |
| Compression strength (kg/ grain pearl) uses primary fermentation after 10 days | 50 15 | 2 0.2 | 0.3 0.1 |
| Store the active half-life (my god) | 120 | 45 | 10 |
| Stationary phase (my god) (immersion of 0.1M phosphate buffer) | 25 | 2 | 1 |
| Burst apart the time started (my god) | Do not have | 4 | 8 |
| The initial embedding of yeast density (hundred million/ml of carrier) fermentation is after 10 days | 46 (doing) 42 | 40 (doing) 35 | 30 (after the propagation) 35 |
| Carrier input amount (%, V/V) | 20 | 20 | 20 |
| Ripe raw spirit part (%, V/V) | 9.0-9.5 | 9.0-9.5 | 9.0-9.5 |
| Alcohol production ability g/L tank/hr. g/L carrier/hr. | 20 40 | 24 44 | 23 42 |
| Carrier service life (my god) | More than 130 days | 18 | 32 |
Claims (4)
1, a kind of alginate calcium volume particle size xerogel pearl of embedding yeast viable cell is characterized in that this xerogel integument is calcium carbonate-filled, and Zhi Di Cause is close, hard, and technical characterstic is:
Dry matter content 83-94% (W/W)
Amount of yeast 6-80 hundred million/ml
Simple grain volume 0.07-3.0cm
3
Equivalent diameter 5-18mm
Proportion 1.2-1.65
Simple grain ultimate compression strength 30-100kg/ grain.
2, a kind of method for preparing the xerogel pearl described in the claim 1, this method will contain zymic sodium alginate soln adding 3-12% (W/V) lime carbonate earlier and make weighting agent, make it to become the mixing mother liquor, it is characterized in that separating moulding system pearl down by liquid, its method is: being provided with flatly under the calcium chloride water liquid level, the footpath is the jet pipe of 8-32mm, order mixes mother liquor and sprays from jet pipe, enter calcium chloride exchange solution, promptly form the protruding capsule of calcium alginate gel film wrapped, when reaching required bead length, in time the protruding capsule of film is squeezed off separation, be shaped to volume particle size liquid capsule, liquid capsule bead under 4 ℃, soaked 24 hours in exchange liquid again, just become the volume particle size gel beads, just make the xerogel pearl after be lower than 35 ℃ of cryodryings processing.
3, require the method described in 2 according to wanting sharp, it is characterized in that described weighting agent lime carbonate add-on is 8%.
4,, it is characterized in that the jet pipe bore under the calcium chloride solution liquid level is 15mm according to the method described in the claim 2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119159A CN1089806C (en) | 1996-10-09 | 1996-10-09 | Fixed yeast xerogel bead and prepn. method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96119159A CN1089806C (en) | 1996-10-09 | 1996-10-09 | Fixed yeast xerogel bead and prepn. method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1155010A true CN1155010A (en) | 1997-07-23 |
| CN1089806C CN1089806C (en) | 2002-08-28 |
Family
ID=5125611
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN96119159A Expired - Fee Related CN1089806C (en) | 1996-10-09 | 1996-10-09 | Fixed yeast xerogel bead and prepn. method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1089806C (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091603A (en) * | 2010-12-30 | 2011-06-15 | 宾丽萍 | Preparation method and application of novel adsorbent resin |
| CN102559803A (en) * | 2012-03-06 | 2012-07-11 | 广西大学 | Fermentation production method of xanthan gum |
| CN105688273A (en) * | 2016-03-18 | 2016-06-22 | 南开大学 | Preparation method for sodium alginate hydrogel hollow tubes |
| CN105833342A (en) * | 2016-03-18 | 2016-08-10 | 南开大学 | Preparation method for sodium alginate hydrogel hollow tube with controllable inner diameter |
| CN108360088A (en) * | 2018-02-28 | 2018-08-03 | 清华大学深圳研究生院 | The method and apparatus for preparing calcium alginate fibre |
| CN109022289A (en) * | 2018-07-04 | 2018-12-18 | 天津农学院 | Pleurotus eryngii solid state bacterial and preparation method thereof |
| CN109022288A (en) * | 2018-07-04 | 2018-12-18 | 天津农学院 | Crab flavour mushroom solid state bacterial and preparation method thereof |
| CN112812912A (en) * | 2021-04-01 | 2021-05-18 | 浙江大学 | Household self-separation beer fermentation barrel and fermentation method thereof |
| CN113073017A (en) * | 2021-04-01 | 2021-07-06 | 浙江大学 | Full-automatic dual-purpose self-brewing intelligent beer machine and method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1064709C (en) * | 1995-04-28 | 2001-04-18 | 广东省微生物研究所 | Yeast fixed carrier and preparation method |
-
1996
- 1996-10-09 CN CN96119159A patent/CN1089806C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102091603A (en) * | 2010-12-30 | 2011-06-15 | 宾丽萍 | Preparation method and application of novel adsorbent resin |
| CN102091603B (en) * | 2010-12-30 | 2015-02-18 | 广东工业大学 | Preparation method and application of novel adsorbent resin |
| CN102559803A (en) * | 2012-03-06 | 2012-07-11 | 广西大学 | Fermentation production method of xanthan gum |
| CN105688273A (en) * | 2016-03-18 | 2016-06-22 | 南开大学 | Preparation method for sodium alginate hydrogel hollow tubes |
| CN105833342A (en) * | 2016-03-18 | 2016-08-10 | 南开大学 | Preparation method for sodium alginate hydrogel hollow tube with controllable inner diameter |
| CN108360088A (en) * | 2018-02-28 | 2018-08-03 | 清华大学深圳研究生院 | The method and apparatus for preparing calcium alginate fibre |
| CN109022289A (en) * | 2018-07-04 | 2018-12-18 | 天津农学院 | Pleurotus eryngii solid state bacterial and preparation method thereof |
| CN109022288A (en) * | 2018-07-04 | 2018-12-18 | 天津农学院 | Crab flavour mushroom solid state bacterial and preparation method thereof |
| CN109022289B (en) * | 2018-07-04 | 2021-11-02 | 天津农学院 | Pleurotus eryngii solid strain and preparation method thereof |
| CN109022288B (en) * | 2018-07-04 | 2021-11-02 | 天津农学院 | Hypsizygus marmoreus solid strain and preparation method thereof |
| CN112812912A (en) * | 2021-04-01 | 2021-05-18 | 浙江大学 | Household self-separation beer fermentation barrel and fermentation method thereof |
| CN113073017A (en) * | 2021-04-01 | 2021-07-06 | 浙江大学 | Full-automatic dual-purpose self-brewing intelligent beer machine and method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1089806C (en) | 2002-08-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1089806C (en) | Fixed yeast xerogel bead and prepn. method thereof | |
| Linko et al. | Continuous ethanol production by immobilized yeast reactor | |
| US20150307822A1 (en) | Process and equipment for multistage, continuous fermentation, with ferment recovery, reactivation and recycling, for producing wines with a high alcohol content | |
| US4892818A (en) | Bioreactor | |
| JP2010094093A (en) | Method for producing ethanol from hull of citrus | |
| JP3594227B2 (en) | Production of fermented products | |
| CN1064709C (en) | Yeast fixed carrier and preparation method | |
| CN1099945A (en) | Process for producing soy sauce with distiller's maize liquid and multienzyme protein feed-stuff with soy sauce dregs | |
| Roukas | Production of citric acid from beet molasses by immobilized cells of Aspergillus niger | |
| CN1114111A (en) | Method of producing liquor | |
| US20120040423A1 (en) | Fermentation | |
| US4904600A (en) | Bioreactor for continuous processing of a reactant fluid | |
| CN1207409A (en) | Method for making fruit wine | |
| CN1218111A (en) | Production of sugar from corn starch and glutamic acid fermentation | |
| Weeks et al. | New concepts for rapid yeast settling. I. Flocculation with an inert powder | |
| EA012642B1 (en) | Continuous method for the production of the yeast fermented beverages | |
| US3737323A (en) | Continuous fermentation process for producing alcoholic beverages | |
| EP2358884A2 (en) | Apparatus and method for continuous fermentation | |
| CN105219805A (en) | A kind of method of high density molasses high-efficiency fermenting alcohol | |
| EP0601362A1 (en) | Process for the production of non-alcoholic beer and device for effecting this process | |
| FR2586256A1 (en) | Process for preparing coated live cells for their biotechnological use | |
| Taillandier et al. | Malate degradation by Schizosaccharomyces yeasts included in alginate beads | |
| CN1717491A (en) | Process for production of ethanol using stable yeast crystals in modified conventional batch reactor | |
| JPS6225986A (en) | Method and apparatus for producing carbon dioxide and ethanol | |
| CN1195065C (en) | Alcohol producing process and reactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |