CN111474342A - Colloidal gold detection card for four-item joint detection of drugs and method for detecting drugs - Google Patents
Colloidal gold detection card for four-item joint detection of drugs and method for detecting drugs Download PDFInfo
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Abstract
The invention provides a four-item joint inspection colloidal gold detection card for drugs and a method for detecting the drugs, relating to the technical field of drug detection; colloidal gold test card includes the casing and places test paper's lower casing, go up the casing and can dismantle with lower casing and be connected, it is equipped with an application of sample hole to go up the casing top, four observation windows and a horizontal scale, be provided with vertical evenly distributed's four independent test paper locating component and horizontal sample stick location passageway in the casing down, the inside space that is used for placing test paper that forms of test paper locating component, test paper locating component is provided with the card strip of fixed test paper, sample stick location passageway corresponds with last casing application of sample hole horizontal direction, be convenient for liquid lateral flow to the application of sample district of every test paper strip. The colloidal gold detection card can be used for simultaneously detecting four drugs by dripping a sample once, is convenient to operate, has high sensitivity and high accuracy, and can improve the use safety.
Description
Technical Field
The invention belongs to the technical field of drug detection, and particularly relates to a four-item joint detection colloidal gold detection card for drugs and a method for detecting the drugs.
Background
The immune colloidal gold technology is a mature immune labeling technology which uses colloidal gold as a tracer marker to be applied to antigen antibodies. Due to the characteristics of low cost, convenient use, quick result reading and the like, the method is widely applied to the field of point of care testing (POCT). At present, colloidal gold test paper strip or test card are mostly a test card and detect an index in the market, and the colloidal gold class product of the immunochromatography that a small number can detect two or more indexes needs to absorb and drip the sample many times, troublesome poeration, and drip many times on a board in addition, must lead to the fact time deviation, influences the interpretation of testing result.
Disclosure of Invention
In view of the above, the present invention provides a colloidal gold detection card for four-item joint detection of drugs and a method for detecting drugs using the same, wherein four detection test paper with different indexes are simultaneously placed at corresponding positions, and a sample is added dropwise to complete all detections, so that the operation is convenient, the sensitivity of the detection card is ensured, the detection accuracy is improved, the environmental pollution is avoided, and the use safety is improved.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a four-item joint inspection colloidal gold detection card for drugs, which comprises an upper shell 1 and a lower shell 2 for placing test strips, wherein the upper shell 1 is detachably connected with the lower shell 2, a sample adding hole 3, 4 observation windows 4 and 1 horizontal scale 5 are arranged above the upper shell 1, 3 columnar supports 6 are arranged on the inner side of the upper shell 1, and 4 independent detection test strip positioning components 7 which are longitudinally and uniformly distributed and sample rod positioning channels 8 which are transversely distributed are arranged in the lower shell 2;
a space for placing the detection test paper is formed in the detection test paper positioning component 7;
the sample adding hole 3 is also provided with a detachable sample adding hole cover plate 31;
a first test strip, a second test strip, a third test strip and a fourth test strip are respectively arranged on the 4 test strip positioning components 7; wherein the first test strip is used for qualitatively detecting morphine; the second detection test strip is used for qualitatively detecting methamphetamine; the third test strip is used for qualitatively detecting ketamine; the fourth test strip is used for qualitatively detecting the dimethylene dioxyamphetamine.
Preferably, a clamping strip 75 for fixing the test paper is arranged on the test paper positioning component 7, and the sample rod positioning channel 8 corresponds to the sample adding hole 3 on the upper shell 1 in the horizontal direction.
Preferably, 4 observation windows 4 correspond to 4 test paper positioning assemblies 7 one-to-one respectively.
Preferably, the horizontal scale 5 is disposed below the observation window 4 and is placed longitudinally.
Preferably, 3 columnar supports 6 on the upper shell 1 respectively correspond to the middle position between every two adjacent test paper positioning assemblies 7 of the lower shell 2.
Preferably, each of the test strip positioning assemblies 7 respectively includes a first U-shaped positioning plate 71, a second U-shaped positioning plate 72, a first bar-shaped positioning plate 73 and an arch-shaped positioning plate 74; the first U-shaped positioning plate 71 and the second U-shaped positioning plate 72 are respectively arranged at two ends of the lower shell 2, the arched positioning plate 74 is arranged in the middle of the first U-shaped positioning plate 71 and the second U-shaped positioning plate 72, and the first strip-shaped positioning plate 73 is arranged at one side close to the second U-shaped positioning plate 72;
the arched positioning plate 74 is arched, and the inner side of the side wall of the first strip-shaped positioning plate 73 is provided with a clamping strip 75.
Preferably, the sample rod positioning channel 8 comprises a third U-shaped positioning plate 81, a fourth U-shaped positioning plate 82, a second strip-shaped positioning plate 83, a third strip-shaped positioning plate 84 and a fourth strip-shaped positioning plate 85, the sample rod positioning channel 8 corresponds to the horizontal direction of the sample adding hole 3 on the upper shell 1, the third U-shaped positioning plate 81 and the fourth U-shaped positioning plate 82 are respectively arranged at two ends near one side of the first U-shaped positioning plate 71, the second strip-shaped positioning plate 83 is arranged at one side near the third U-shaped positioning plate 81, the third strip-shaped positioning plate 84 is arranged at the middle position between the third U-shaped positioning plate 81 and the fourth U-shaped positioning plate 82, the fourth strip-shaped positioning plate 85 is arranged at one side near the fourth U-shaped positioning plate 82, the middle position of the third strip-shaped positioning plate 84 is right opposite to the sample adding hole 3 of the upper shell 1, the top ends of the side walls of the second strip-shaped positioning plate 83 and the fourth strip-shaped positioning plate 85 are both provided with a clamping strip 86.
Preferably, the first test strip is coated with morphine antigen and labeled with morphine antibody;
the second detection test strip is coated with a methamphetamine antigen and a methamphetamine antibody for marking;
the third test strip comprises ketamine antigen and a labeled ketamine antibody;
the fourth test strip is coated with a dimethylene dioxyamphetamine antigen and labeled with a dimethylene dioxyamphetamine antibody.
The invention also provides the application of the colloidal gold detection card in simultaneously detecting four drugs.
The invention also provides a method for detecting four drugs, which comprises the following steps of dropwise adding a 500 mu L urine sample into the sample adding hole 3 of the colloidal gold detection card, covering the sample adding hole cover plate 31, standing for 5min, and respectively judging the types of drugs sucked by a urine sample provider according to the color development conditions of the detection test strips.
The invention provides a four-item joint inspection colloidal gold detection card for drugs, wherein four detection test paper positioning components 7 of a lower shell 2 of the colloidal gold detection card correspond to four observation windows 4 of an upper shell 1, and can simultaneously detect and read four indexes of the same sample. Four observation windows 4 are corresponding to a application of sample hole 3, and the application of sample hole outside is provided with application of sample hole apron 31, can prevent effectively that the liquid of adding from spattering outward, guarantees the accuracy that detects, and keeps surrounding environment safety, clean and tidy. The sample adding hole 3 corresponds to the sample rod positioning channel 8 of the lower shell 2, and the added liquid is conveyed to each sample pad for sample detection through the diffusion effect of the sample rod. Three column supports 6 of the upper shell 1 are used for supporting the middle position of the detection card and ensuring the stability of the whole shell. Go up horizontal scale 5 of casing 1 and can observe whether the detection card is in the horizontality, increase the sensitivity, guarantee the accuracy of testing result.
Drawings
Fig. 1 is a schematic top view of the colloidal gold test card, in which 1 represents an upper case, 2 represents a lower case, 3 represents a well, 31 represents a well cover plate, 4 represents an observation window, 5 represents a horizontal scale, and 9 represents an anti-skid pattern;
fig. 2 is a schematic front structure view of an upper case of the colloidal gold test card;
fig. 3 is a schematic structural view of the back surface of the upper case of the colloidal gold test card, in which 6 denotes a columnar support, 10 denotes a strip-shaped sample pressing ridge, 11 denotes a dot-shaped sample pressing ridge, and 12 denotes a card column;
fig. 4 is a schematic structural diagram of a lower housing of the colloidal gold test card, in which 7 denotes a test paper positioning assembly, 8 denotes a sample rod positioning passage, 13 denotes a card slot, 71 denotes a first U-shaped positioning plate, 72 denotes a second U-shaped positioning plate, 73 denotes a first bar-shaped positioning plate, 74 denotes an arch-shaped positioning plate, 75 denotes a card bar, 81 denotes a third U-shaped positioning plate, 82 denotes a fourth U-shaped positioning plate, 83 denotes a second bar-shaped positioning plate, 84 denotes a third bar-shaped positioning plate, 85 denotes a fourth bar-shaped positioning plate, and 86 denotes a card bar.
Detailed Description
The invention provides a four-item joint inspection colloidal gold detection card for drugs, which comprises an upper shell 1 and a lower shell 2 for placing test strips, wherein the upper shell 1 is detachably connected with the lower shell 2, a sample adding hole 3, 4 observation windows 4 and 1 horizontal scale 5 are arranged above the upper shell 1, 3 columnar supports 6 are arranged on the inner side of the upper shell 1, and 4 independent detection test strip positioning components 7 which are longitudinally and uniformly distributed and sample rod positioning channels 8 which are transversely distributed are arranged in the lower shell 2;
a space for placing the detection test paper is formed in the detection test paper positioning component 7;
the sample adding hole 3 is also provided with a detachable sample adding hole cover plate 31;
a first test strip, a second test strip, a third test strip and a fourth test strip are respectively arranged on the 4 test strip positioning components 7; wherein the first test strip is used for qualitatively detecting morphine; the second detection test strip is used for qualitatively detecting methamphetamine; the third test strip is used for qualitatively detecting ketamine; the fourth test strip is used for qualitatively detecting the dimethylene dioxyamphetamine.
The structure of the colloidal gold detection card is shown in figures 1-4, and a sealed sample adding hole cover plate 31 is arranged on the outer side of the sample adding hole 3, so that the added liquid can be effectively prevented from splashing, the detection accuracy is ensured, and the safety and tidiness of the surrounding environment are kept.
According to the invention, one sample adding hole 3 of the upper shell 1 corresponds to four observation windows 4, and the four observation windows respectively correspond to four detection test paper positioning components 7 of the lower shell, so that a sample is added dropwise at one time, and four indexes of the same sample are detected and read at the same time.
According to the invention, the horizontal scale 5 of the upper shell 1 is arranged at the lower position of the observation window and is arranged along the longitudinal direction, so that whether the detection card is in a horizontal state or not can be observed, the sensitivity is increased, and the accuracy of the detection result is ensured.
The three columnar supports 6 on the inner side of the upper shell 1 preferably respectively correspond to the middle position between every two test paper positioning components 7 of the lower shell 2, and are used for supporting the middle position of a test card so as to ensure the stability of the whole shell.
In the invention, the detection test paper positioning component 7 is preferably provided with a clamping strip 75 for fixing the detection test paper, and the sample rod positioning channel 8 preferably corresponds to the horizontal direction of the sample adding hole 3 on the upper shell 1. The test paper positioning assembly 7 of the present invention preferably includes a first U-shaped positioning plate 71, a second U-shaped positioning plate 72, a first bar-shaped positioning plate 73, and an arch-shaped positioning plate 74, respectively. First U-shaped locating plate 71 and second U-shaped locating plate 72 set up respectively in the both ends of lower casing 2, and arch locating plate 74 sets up in the intermediate position of first U-shaped locating plate 71 and second U-shaped locating plate 72, and first bar locating plate 73 sets up in the one side that is close to second U-shaped locating plate 72, and arch locating plate 74 is the arch, and first bar locating plate 73 lateral wall inboard is provided with card strip 75. In the invention, strip-shaped sample pressing ridges 10 and dot-shaped sample pressing ridges 11 are preferably respectively arranged at two ends of the detection test paper positioning component 7. The detection test paper positioning component 7 can better position the detection test paper.
In the test paper positioning assembly 7 of the present invention, the first U-shaped positioning plate 71 and the second U-shaped positioning plate 72 are mainly used for fixing the longitudinal position of the test paper, the first bar-shaped positioning plate 73 and the clamping strip 75 on the inner side of the sidewall thereof are mainly used for fixing the transverse position of the test paper, the arch-shaped positioning plate 74 is mainly used for fixing the upper and lower positions in the middle of the test paper, and the bar-shaped indentation ridge 10 and the dot-shaped indentation ridge 11 are mainly used for fixing the upper and lower positions at the two ends of the test paper.
The sample rod positioning channel 8 according to the present invention preferably comprises a third U-shaped positioning plate 81, a fourth U-shaped positioning plate 82, a second strip-shaped positioning plate 83, a third strip-shaped positioning plate 84 and a fourth strip-shaped positioning plate 85, sample stick location passageway 8 corresponds with last casing application of sample hole 3 horizontal direction, and third U-shaped locating plate 81 and fourth U-shaped locating plate 82 set up respectively in being close to the both ends of one side of first U-shaped locating plate 71, and second bar locating plate 83 sets up in the one side of being close to third U-shaped locating plate 81, and third bar locating plate 84 sets up in the intermediate position of third U-shaped locating plate 81 and fourth U-shaped locating plate 82, and fourth bar locating plate 85 sets up in the one side of being close to fourth U-shaped locating plate 82, and third bar locating plate 84 intermediate position is just to the application of sample hole 3 of last casing, and second bar locating plate 83 and fourth bar locating plate 85 lateral wall top all are provided with card strip 86. This arrangement allows liquid entering the well 3 to be transmitted to the sample pad of each test strip by diffusion through the sample rod 8, and the samples are tested item by item.
In the sample rod positioning channel 8, the third U-shaped positioning plate 81 and the fourth U-shaped positioning plate 82 are mainly used for fixing the longitudinal position of a sample rod, the second strip-shaped positioning plate 83, the third strip-shaped positioning plate 84 and the fourth strip-shaped positioning plate 85 are mainly used for fixing the transverse position of the sample rod, and the clamping strips 86 are arranged at the top ends of the side walls of the second strip-shaped positioning plate 83 and the fourth strip-shaped positioning plate 85 and are mainly used for fixing the upper position and the lower position of the sample rod.
The anti-skid grains 9 are preferably arranged on the two sides of the lower shell, so that the hand holding is firmer.
In the invention, the upper shell 1 and the lower shell 2 are preferably detachably connected through the clamping columns 12 and the clamping grooves 13, the clamping columns 12 are preferably positioned on the periphery of the upper shell 1, the clamping grooves 13 are positioned on the periphery of the lower shell 2, the clamping columns 12 and the clamping grooves 13 are in one-to-one correspondence, and the clamping columns 12 can be inserted into the clamping grooves 13, so that the upper shell 1 and the lower shell 2 are fixed.
The first test strip is preferably coated with morphine antigen and labeled morphine antibody for qualitatively detecting morphine (heroin and opium) content in urine, the second test strip is preferably coated with methamphetamine antigen and labeled methamphetamine antibody for qualitatively detecting methamphetamine (methamphetamine) in urine, the third test strip is preferably coated with ketamine antigen and labeled ketamine antibody for qualitatively detecting ketamine (K powder) in urine, the fourth test strip is preferably coated with dimethylene dioxy amphetamine antigen and labeled dimethylene dioxy amphetamine antibody for qualitatively detecting dimethylene dioxy amphetamine (ecstasy) in urine, the sources of the coated antigen and the labeled antibody on the test strip are not particularly limited, the test strip is prepared or directly purchased by a conventional method in the field, in the embodiment of the invention, the labeled morphine antibody, the labeled methamphetamine antibody, the labeled diethylethamine antibody and the labeled dioxy antibody are prepared or directly purchased by a conventional method in the field, a large amount of monoclonal antibody for immunizing mouse with morphine antigen, monoclonal antibody for selectively hybridizing with monoclonal antibody, monoclonal antibody for detecting mouse monoclonal antibody, monoclonal antibody.
The invention also provides the application of the colloidal gold detection card in simultaneously detecting four drugs. When the colloidal gold detection card is used for detecting drugs, the detection principle is as follows: firstly, a horizontal scale 5 is utilized to ensure that the detection card is placed at a horizontal position, then a sample is dropwise added from the sample adding hole 3, the sample rod is soaked, the sample flows through the sample rod to reach the position of a sample pad of each detection test paper through the diffusion effect of the sample rod, the sample is transferred to the sample pad, and the detection of indexes one by one is respectively carried out according to the reaction principle of the detection test paper.
The invention also provides a method for detecting four drugs, which comprises the following steps of dropwise adding a 500 mu L urine sample into the sample adding hole 3 of the colloidal gold detection card, covering the sample adding hole cover plate 31, standing for 5min, and respectively judging the types of drugs sucked by a urine sample provider according to the color development conditions of the detection test strips.
The reaction principle of each test paper is the same: and detecting the specific drug antigen in the urine by adopting a colloidal gold immunochromatography. The reagent contains an antigen and a colloidal gold-labeled antibody which are immobilized in advance on a test zone (T) on the membrane. In the test, urine samples were chromatographed upward under capillary effect after soaking in test paper. If the urine is negative, no detected substance in the urine is combined with the colloidal gold antibody, the colloidal gold antibody reacts with the antigen, and a purple red strip appears on the detection line. If the urine sample is positive, the detected substance in the urine reacts with the colloidal gold antibody firstly, when the content of the detected substance in the urine reaches a certain amount, the colloidal gold antibody is not combined with the specific antigen in the test area any more, and the detection line has no purple red strip. The control line shows a purple-red band regardless of the presence of the substance to be detected in the urine. The purple red band shown by the quality control line is a standard for judging whether enough urine sample exists or not and whether the chromatographic process is normal or not, and is also used as an internal control standard of the reagent. The quality control line is used as an internal control standard of the test paper, if a purple red strip appears, the test result is accurate, and if no purple red strip appears, the test result is wrong. The test line is used as a detection standard, if a purple red strip appears, the test result is negative, namely the detected person does not take poison, and if the purple red strip does not appear, the test result is positive, namely the detected person takes poison.
The four-item joint inspection colloidal gold test card and the test method of the drug of the present invention will be described in detail with reference to the following examples, but they should not be construed as limiting the scope of the present invention.
Example 1
The utility model provides a four joint inspection colloidal gold detects card, the structure is shown in fig. 1 ~ 4, including last casing 1 and the lower casing 2 of placing the test paper strip, go up casing 1 and casing 2 down and can dismantle through a plurality of card post 12 and draw-in groove 13 that correspond each other and be connected, it is provided with a application of sample hole 3 to go up casing 1 top, four observation windows 4 and a horizontal scale 5, it is provided with three column support 6 to go up casing 1 inboard, be provided with vertical evenly distributed's four independent test paper locating component 7 and horizontal sample stick location passageway 8 in the lower casing, the inside space that is used for placing test paper that forms of test paper locating component 7, test paper locating component is provided with the card strip 75 of fixed test paper, sample stick location passageway 8 corresponds with the 3 horizontal directions in application of sample hole of last casing 1.
The four detection test paper positioning components are respectively used for placing a first detection test paper strip, a second detection test paper strip, a third detection test paper strip and a fourth detection test paper strip. Wherein the content of the first and second substances,
the first test strip was coated with morphine antigen (purchased from EastCoastBos), labeled with morphine antibody (self-produced, morphine 3G)9A11E10B2) For qualitative detection of morphine (heroin, opium) content in urine;
the second test strip was coated with methamphetamine antigen (purchased from EastCoast Bio), labeled with methamphetamine antibody (self-produced, methamphetamine 4G)3B7C8G8B2) For qualitative detection of methamphetamine (methamphetamine) in urine;
the third test strip was coated with ketamine antigen (purchased from EastCoast Bio), labeled ketamine antibody (self-produced, ketamine 2B)3D6E6F7) For qualitative detection of ketamine (K powder) in urine;
the fourth test strip was coated with a dimethylene dioxyamphetamine antigen (ex EastCoast Bio), labeled with a dimethylene dioxyamphetamine antibody (ex-Produced, dimethylene dioxyamphetamine 3E)7H6G6F3) The kit is used for qualitatively detecting dimethylene dioxyamphetamine (dancing outreach pill) in urine.
The sealed cover plate 31 is arranged on the outer side of the sample adding hole 3, so that added liquid can be effectively prevented from splashing outwards, the detection accuracy is guaranteed, and the safety and tidiness of the surrounding environment are kept.
The sample adding hole 3 of the upper shell corresponds to the four observation windows 4, and the four observation windows respectively correspond to the four detection test paper positioning components 7 of the lower shell, so that a one-time dropwise adding sample is realized, and four indexes of the same sample are detected and read simultaneously.
The horizontal scale 5 of going up the casing sets up the below position at the observation window, and vertical setting can observe whether the detection card is in the horizontality, increases the sensitivity, guarantees the accuracy of testing result.
The three columnar supports 6 on the inner side of the upper shell correspond to the middle positions between every two positioning components of the lower shell respectively and are used for supporting the middle positions of the detection cards and ensuring the stability of the whole shell.
The test paper positioning assembly comprises a first U-shaped positioning plate 71, a second U-shaped positioning plate 72, a first bar-shaped positioning plate 73 and an arch-shaped positioning plate 74. First U-shaped locating plate 71 and second U-shaped locating plate 72 set up respectively in the both ends of lower casing 2, and arch locating plate 74 sets up in the intermediate position of first U-shaped locating plate 71 and second U-shaped locating plate 72, and first bar locating plate 73 sets up in the one side that is close to second U-shaped locating plate 72, and arch locating plate 74 is the arch, and first bar locating plate 73 lateral wall inboard is provided with card strip 75. This series of settings is intended to better position the test strip.
Detection test paper locating component: the first U-shaped positioning plate 71 and the second U-shaped positioning plate 72 are mainly used for fixing the longitudinal position of the test paper, the first bar-shaped positioning plate 73 and the clamping strip 75 on the inner side of the side wall thereof are mainly used for fixing the transverse position of the test paper, the arch-shaped positioning plate 74 is mainly used for fixing the upper and lower positions in the middle of the test paper, and the bar-shaped indentation ridge 10 and the dot-shaped indentation ridge 11 are mainly used for fixing the upper and lower positions at the two ends of the test paper.
The sample rod positioning channel comprises a third U-shaped positioning plate 81, a fourth U-shaped positioning plate 82, a second strip-shaped positioning plate 83, a third strip-shaped positioning plate 84 and a fourth strip-shaped positioning plate 85, the sample rod positioning channel 8 corresponds to the horizontal direction of the sample adding hole 3 of the upper shell, the third U-shaped positioning plate 81 and the fourth U-shaped positioning plate 82 are respectively arranged at two ends of one side of the first U-shaped positioning plate 71, the second strip-shaped positioning plate 83 is arranged at one side close to the third U-shaped positioning plate 81, the third strip-shaped positioning plate 84 is arranged at the middle position of the third U-shaped positioning plate 81 and the fourth U-shaped positioning plate 82, the fourth strip-shaped positioning plate 85 is arranged at one side close to the fourth U-shaped positioning plate 82, the middle position of the third strip-shaped positioning plate 84 is just opposite to the sample adding hole 3 of the upper shell, and the side wall top ends. This arrangement allows liquid entering the well 3 to be transmitted to the sample pad of each test strip by diffusion through the sample rod 8, and the samples are tested item by item.
Sample rod positioning channel: the third U-shaped positioning plate 81 and the fourth U-shaped positioning plate 82 are mainly used for fixing the longitudinal position of the sample rod, the second strip-shaped positioning plate 83, the third strip-shaped positioning plate 84 and the fourth strip-shaped positioning plate 85 are mainly used for fixing the transverse position of the sample rod, and the clamping strips 86 are arranged at the top ends of the side walls of the second strip-shaped positioning plate 83 and the fourth strip-shaped positioning plate 85 and are mainly used for fixing the upper position and the lower position of the sample rod.
The two sides of the lower shell are provided with anti-skid grains 9, so that the hand holding is firmer.
Example 2
Randomly drawing 50 persons in a certain entertainment place to measure the urine, dropwise adding 500 mu L urine samples to the positions of the sample adding holes 3 of the detection card in the embodiment 1, then covering the sample adding hole cover plate 31, standing for 5min at room temperature, simultaneously carrying out comparison tests on each single index respectively, and observing the color development conditions of a quality control line and a detection line at regular time.
Simultaneously, setting a contrast test: and detecting the same urine sample by using four corresponding pairs of commercially available single-pack drug detection cards.
The detection shows that the urine sample of 3 persons shows positive result in the third test strip of the test card, and the result is consistent with the result of the contrast test, and the test card has no obvious deviation in color development condition and time in the whole test process. Meanwhile, the detection result is also verified by public security authorities. The colloidal gold detection card provided by the invention avoids busy repeated dripping, shortens time difference, and is simple to operate, time-saving and efficient.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. The colloidal gold detection card for the four-item joint detection of drugs is characterized by comprising an upper shell (1) and a lower shell (2) for placing test strips, wherein the upper shell (1) is detachably connected with the lower shell (2), a sample adding hole (3), 4 observation windows (4) and 1 horizontal scale (5) are arranged above the upper shell (1), 3 columnar supports (6) are arranged on the inner side of the upper shell (1), and 4 independent detection test strip positioning components (7) which are longitudinally and uniformly distributed and sample rod positioning channels (8) which are transversely distributed are arranged in the lower shell (2);
a space for placing the detection test paper is formed in the detection test paper positioning component (7);
a detachable sample adding hole cover plate (31) is also arranged on the sample adding hole (3);
a first detection test strip, a second detection test strip, a third detection test strip and a fourth detection test strip are respectively arranged on the 4 detection test strip positioning components (7); wherein the first test strip is used for qualitatively detecting morphine; the second detection test strip is used for qualitatively detecting methamphetamine; the third test strip is used for qualitatively detecting ketamine; the fourth test strip is used for qualitatively detecting the dimethylene dioxyamphetamine.
2. The colloidal gold test card according to claim 1, wherein a strip (75) for fixing the test paper is disposed on the test paper positioning component (7), and the sample rod positioning channel (8) corresponds to the sample adding hole (3) of the upper case (1) in the horizontal direction.
3. The colloidal gold test card according to claim 1, wherein 4 observation windows (4) are respectively corresponding to 4 test paper positioning components (7).
4. The colloidal gold test card according to claim 1, wherein said horizontal scale (5) is disposed below the observation window (4) and is placed longitudinally.
5. The colloidal gold test card according to claim 1, wherein the 3 columnar supports (6) on the upper case (1) correspond to the middle position between every two adjacent test paper positioning components (7) of the lower case (2).
6. The colloidal gold test card according to claim 1 or 5, wherein each test paper positioning assembly (7) comprises a first U-shaped positioning plate (71), a second U-shaped positioning plate (72), a first strip-shaped positioning plate (73) and an arch-shaped positioning plate (74); the first U-shaped positioning plate (71) and the second U-shaped positioning plate (72) are respectively arranged at two ends of the lower shell (2), the arched positioning plate (74) is arranged in the middle of the first U-shaped positioning plate (71) and the second U-shaped positioning plate (72), and the first strip-shaped positioning plate (73) is arranged at one side close to the second U-shaped positioning plate (72);
the arched positioning plate (74) is arched, and the inner side of the side wall of the first strip-shaped positioning plate (73) is provided with a clamping strip (75).
7. The colloidal gold detection card according to claim 1, wherein the sample rod positioning channel (8) comprises a third U-shaped positioning plate (81), a fourth U-shaped positioning plate (82), a second strip-shaped positioning plate (83), a third strip-shaped positioning plate (84) and a fourth strip-shaped positioning plate (85), the sample rod positioning channel (8) corresponds to the sample adding hole (3) on the upper housing (1) in the horizontal direction, the third U-shaped positioning plate (81) and the fourth U-shaped positioning plate (82) are respectively arranged at two ends near one side of the first U-shaped positioning plate (71), the second strip-shaped positioning plate (83) is arranged at one side near the third U-shaped positioning plate (81), the third strip-shaped positioning plate (84) is arranged at the middle position between the third U-shaped positioning plate (81) and the fourth U-shaped positioning plate (82), fourth shape locating plate (85) set up in being close to one side of fourth U-shaped locating plate (82), third bar locating plate (84) intermediate position is just right go up application of sample hole (3) of casing (1), second bar locating plate (83) and fourth shape locating plate (85) lateral wall top all are provided with card strip (86).
8. The colloidal gold test card of claim 1, wherein the first test strip is coated with a morphine antigen, a labeled morphine antibody;
the second detection test strip is coated with a methamphetamine antigen and a methamphetamine antibody for marking;
the third test strip comprises ketamine antigen and a labeled ketamine antibody;
the fourth test strip is coated with a dimethylene dioxyamphetamine antigen and labeled with a dimethylene dioxyamphetamine antibody.
9. Use of the colloidal gold test card according to any one of claims 1 to 8 for simultaneously testing four drugs.
10. A method for simultaneously detecting four drugs is characterized by comprising the following steps of dropwise adding a 500 mu L urine sample into a sample adding hole (3) of the colloidal gold detection card according to any one of claims 1 to 8, covering a sample adding hole cover plate (31), standing for 5min, and judging the types of drugs sucked by a urine sample provider according to the color development conditions of the detection test strips.
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Cited By (1)
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| CN113607721A (en) * | 2021-06-01 | 2021-11-05 | 陈思行 | Detection card for detecting drug components in liquid food |
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