CN104422762A - Drug testing enzyme linked immunosorbent assay kit and detection method thereof - Google Patents
Drug testing enzyme linked immunosorbent assay kit and detection method thereof Download PDFInfo
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Abstract
The invention discloses an enzyme linked immunosorbent assay kit for detecting drugs in urine, saliva, blood and hairs in the technical field of the prohibited goods detection and analysis as well as a detection method of the drug testing enzyme linked immunosorbent assay kit. The kit comprises a drug standard substance solution, a drug specificity antibody, an elisa plate which is coated with the drug specificity antibody, a drug antigen enzyme marker, a color developing agent, concentrated washing liquor, a termination solution and a sample dilute solution. The enzyme linked immunosorbent assay kit has characteristics of high specificity, high sensitivity, high accuracy and the like and plays an important role in drug testing.
Description
Technical field
The invention belongs to contraband detecting analysis technical field, be specifically related to a kind of enzyme linked immunological kit and the detection method thereof that detect drugs in urine, saliva, blood, hair.
Background technology
Under international narcotics tide spreads unchecked impact, the drugs situation of China is very severe, the statistical figure in by the end of May, 2013 by the end of, and Chinese current drug abuse colony 2,220,000 people also has many recessive drug addicts in addition.Therefore, in order to prevent and punish drugs criminal offence, protection citizen is physically and mentally healthy, safeguards the work of civil order, sets up accurately and reliably, highly sensitive illicit drugs inspection is very necessary.
Opium class drugs mostly cause unreal property and narcoticness, have analgesic activity, mainly comprise opium, hemp, heroin, morphine, 6-monoacetylmorphine, codeine etc.Capillary zone electropheresis refers to amphetamine the compound of the class Prof. Du Yucang being parent.In the psychotropic substances of China's control, belong to the substitutive derivative 3 had on amphetamine, crystal methamphetamine (methamphetamine), in addition its phenyl ring of such drugs, 4-methylene-dioxy amphetamine, MDMA (head-shaking pill) etc.KET, medically claims ketamine, belongs to intravenous anesthesia medicine, has certain psychic dependence.After KET habituation, under drugs effect, smoker understands madness and shakes the head, and is easy to shake disconnected cervical vertebra; Meanwhile, waving of madness also can cause mental and physical efforts, respiratory failure.Suck excessive or suck for a long time, all can cause fatal damage to the heart, lung, nerve, also more severe than methamphetamine to the damage of nervous centralis.Show according to State Food and Drug Administration issues in recent years " drug abuse surveillance report ", what China was mainly subject at present is based on traditional drugs of morphine, heroin, Sauteralgyl etc. with based on the harm of the novel drug of methamphetamine, head-shaking pill, KET, caffeine etc.
Current report mainly contains vapor-phase chromatography (GC), GC-MS(gas chromatography-mass spectrography) (GC/MS), high performance liquid chromatography (-HPLC), tablets by HPLC-MS (HPLC/MS), capillary electrophoresis (CE) and immunoassay (IA) etc. for the method detecting drugs.Chromatography is the detection method generally adopted in the world at present, highly sensitive, accuracy good, the confirmation method that Chang Zuowei medicament residue detects, but it requires all higher to instrument and operating personnel, cost is also higher, a sample detection wants hundreds of unit, and detection time is also very long, is unfavorable for promoting the use of in grass-roots unit.Immunoassay is a kind of drugs screening technique general in the world.At present conventional colloidal gold method is by the impact of biopreparate used, and the probability that false positive occurs is very high, and due to colloidal gold method sensitivity low, color judges have human factor to cause poor accuracy.
Therefore it is a kind of quick, sensitive, accurate to set up, and high volume applications can have very important realistic meaning in the method for illicit drugs inspection.
Summary of the invention
The object of the present invention is to provide a kind of enzyme linked immunological kit detecting drugs in urine, saliva, blood, hair, solve the technical matters of current detection drugs method " fast and ineffective, spirit and unhappy ".
The present invention also aims to provide a kind of and apply the method that mentioned reagent box carries out illicit drugs inspection.
A kind of illicit drugs inspection enzyme linked immunological kit, comprises following material: drugs standard solution, drugs specific antibody; Be coated with the ELISA Plate of drugs specific antibody; Drugs antigen enzyme marker; Developer; Concentrated cleaning solution; Stop buffer and sample diluting liquid.
Described drugs are morphine, ketamine, hemp, heroin, 6-monoacetylmorphine, codeine or amphetamine compound.
Described amphetamine compound is amphetamine, crystal methamphetamine, 3,4-methylene-dioxy amphetamine or MDMA.
Described drugs specific antibody is the conjugate synthesized by carbodiimide method with drugs and carrier protein, and described carrier protein is mouse haemocyanin, bovine serum albumin, thyroprotein, rabbit serum proteins or hemocyanin.
Marker enzyme in described drugs antigen enzyme marker is horseradish peroxidase or alkaline phosphatase.
Described developer is o-phenylenediamine or tetramethyl benzidine.
Described concentrated cleaning solution is for containing 0.5% Tween-20, the phosphate buffer of 0.01mol/L; Stop buffer is the sulfuric acid of 1-2mol/L, hydrochloric acid or sodium hydrate buffer solution; Sample diluting liquid is the phosphate buffer of 0.01mol/L.
Adopt mentioned reagent box to carry out the method for illicit drugs inspection, carry out in accordance with the following steps:
(1) sample pre-treatments
When test sample is urine, saliva, during blood, directly detects; When test sample is hair, first clean with methanol solution, the fragment that rear scissors is cut into 1-10mm is dried in cleaning, take the above-mentioned hair fragment of 2-20mg, add 0.5-2ml methanol solution, 70-75 DEG C of water-bath is static after two hours is cooled to room temperature, get supernatant 37 DEG C of nitrogen to dry up, residue 100-200ul sample diluting liquid dilution, on oscillator, vibration is fully dissolved, to be detected;
(2) in the ELISA Plate being coated with drugs specific antibody, add sample solution or drugs standard solution and drugs antigen enzyme marker solution incubated at room 15-30 minute, the drugs antibody in ELISA Plate competed by drugs in testing sample and the drugs of enzyme labeling, wash plate 3 times, by the unconjugated enzyme marker of washing removing, add developer incubated at room 5-15 minute, stop with stop buffer after colour developing, the absorbance of microplate reader working sample;
(3) in the absorbance that obtains of step (2) and sample, the amount of drugs, in negative relation, compares the concentration that can draw drugs in sample with typical curve.
Beneficial effect of the present invention: illicit drugs inspection enzyme linked immunological kit of the present invention can qualitative and quantitative analysis urine, saliva, blood, the content of drugs in hair, kit requires low to the pre-treatment of sample, sample pretreatment process is simple, can detect gross sample fast simultaneously; Main employing competitive ELISA determination method, operation steps loaded down with trivial details in former euzymelinked immunosorbent assay (ELISA) is carried out simplify, optimize, main agents provides again as a solution, can reduce the operation steps of kit, saves time and reduce the error caused because of complex operation step for user; According to the depth of the color sample in ELISA Plate, can compare with the color of drugs standard items series concentration simultaneously, do not need instrument can judge the concentration range of drugs, in qualitative detection, require low to instrument and equipment, on-the-spot monitoring can be realized; The kit retention time is long, automaticity is high, cost is low, "dead" isotopic contamination, through showing the preci-sion and accuracy test experiments of kit, enzyme linked immunological kit of the present invention has the features such as high specific, high sensitivity, pin-point accuracy, will play a significant role in illicit drugs inspection.
Accompanying drawing explanation
Fig. 1 is opium class examination criteria curve map.
Fig. 2 is amphetamine examination criteria curve map.
Fig. 3 is ketamine examination criteria curve map.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention will be further described.
The preparation of embodiment 1 reagent constituents
(1) synthesis of antigen
A. the synthesis of morphine artificial antigen
Take morphine starting material 500mg, add 50mL benzene and 1g succinic anhydride, add hot reflux 8h.Install vaporising device, boil off benzene.Precipitation uses absolute ether, absolute ethanol washing, residue vacuum drying respectively, namely obtains powdered substance 6-succinic acid-morphine.Take above-mentioned powdered substance 20mg to be dissolved in 10ml PBS damping fluid (phosphate buffer), add 30ul triethylamine, then add 30ul isobutyl chlorocarbonate, react half an hour.Take 20mg BSA to be dissolved in PBS damping fluid, add above-mentioned reactant liquor, stirring reaction spends the night, and dialyse 3 days, high speed freezing centrifuge is centrifugal, collects supernatant, obtains morphine antigen.
B. the synthesis of crystal methamphetamine artificial antigen
Taking methamphetamine hydrochloride raw material 350mg is dissolved in distilled water, and the NaOH solution dripping 1mol/L is completely free out to crystal methamphetamine, adds anhydrous Na
2sO
4after dried overnight, then add chloroform extraction, isolate free crystal methamphetamine grease, be dissolved in appropriate absolute ethyl alcohol, add the succinic anhydride of about 200mg, ice bath cools, and adds about 1g aluminum trichloride (anhydrous).Potpourri stirs 48h under ice bath, then drips the hydrochloric acid hydrolysis of 6mol/L under lower than the condition of 10 DEG C.Until the solid matter of condensation shape sticks in flask walls, divide and remove dichloromethane layer.Add appropriate water, drip alkali lye and be adjusted to pH=14, evaporate to dryness and get final product after chloroform extraction.Product is dissolved in a small amount of water, adds 13.5mgNHS, 20.24mgEDC, complete after stirring 3h.Getting 18.32mgBSA is dissolved in 2mlPBS (0.025mol/L, pH=7.4), is instilled wherein by the solution completed before, coupling 7h in stirring.Last in PBS dislysate low temperature dialysis 3 days, high speed freezing centrifuge is centrifugal, collects supernatant, obtains crystal methamphetamine antigen.
C. the synthesis of ketamine artificial antigen
Take ketamine hydrochloride raw material 274mg, be extracted with ethyl acetate after alkali tune and obtain ketamine free alkali.Ketamine is dissolved in 20ml toluene, add 200mg triethylamine, ice bath cools, and adjusts pH to 12 with dilute NaOH solution, separate organic phase, be extracted with ethyl acetate, water washing organic phase twice, collect organic phase, anhydrous sodium sulfate drying 1h, join in methyl alcohol except after desolventizing, and add reductive agent 200mg reaction and spend the night, through aftertreatment and get final product.Mixed with KOH, tetrabutylammonium iodide, KI and N-4-brombutyl phthalimide by above-mentioned substance, add acetonitrile, temperature rising reflux spends the night, and next day, removal of solvent under reduced pressure, added water and ethyl acetate, layering, collects organic phase, removal of solvent under reduced pressure.Above-mentioned substance is obtained ketamine haptens after hydrazinolysis.Getting ketamine haptens 20mg is dissolved in 1mLDMF, adds the solution of coupling protein BSA and damping fluid PBS (0.01mol/L, pH=7.4) 2ml, adds glutaraldehyde, stirring at room temperature 4h, and 4 DEG C of placements are spent the night, and dialyses two days after taking-up in PBS solution.High speed freezing centrifuge is centrifugal, collects supernatant, obtains ketamine antigen.
(2) preparation of antibody
Choose the healthy Male New Zealand White Rabbit of 6 body weight 2-2.5kg.The Freund's complete adjuvant of the above-mentioned antigen for preparing and equivalent is mixed into water in oil emulsion by syringe to the method for taking out, carries out first immunisation by the amount of 1mg/kg body weight, take dorsal sc multi-point injection.Every two weeks booster immunizations once, replace Freund's complete adjuvant with incomplete Freund's adjuvant, dosage and the same first immunisation of method.From third time immunity, latter 10 days of each immunity, auricular vein gets blood 1ml, carries out antibody titer detection, when antibody titer no longer raises, do not add adjuvant and carry out last immunity, leg muscle is injected, rear neck artery bloodletting in 7 days, after room temperature solidifies 2h, 4 DEG C are spent the night, centrifugal 10 minutes of 8000r/min, removing clot, extracts serum.After dialysis, use SephadexG-25M chromatography, obtain antibody.
(3) preparation of ELISA Plate
A. bag is buffered liquid: the carbonate buffer solution (CBS) of pH=9.6,0.05mol/L.
B. confining liquid: containing the polyglycol of 3%, the casein of 1%, the glycocoll of 4%, the ovalbumin of 1%, the phosphate buffer of the gelatin of 1%.
Liquid is buffered by the antibody dilution in above-mentioned (2) to 1ug/ml with bag, every hole adds 100ul, hatch 2h or 4 DEG C for 37 DEG C to spend the night, remove coating buffer, wash 3 times with cleansing solution, pat dry, then 200ul confining liquid is added in every hole, 37 DEG C of incubation 2h, remove liquid in hole, preserve after drying with aluminum foil under vacuum sealing.
(4) preparation of drugs antigen enzyme marker
Taking horseradish peroxidase 25mg is dissolved in 1.25% glutaraldehyde solution, and in room temperature hold over night, through Sephadex G-25 chromatographic column, use physiological saline wash-out, flow control, at 1ml/min, collects brown efflux, is concentrated into 5ml with poly-diethanol.Take the drugs antigen of 12.5mg, with normal saline dilution to 5ml, dropwise add under stirring in appeal concentrate.Then add the CBS0.25ml of 1mol/L, Keep agitation 3h, add the lysine 0.25ml of 0.2mol/L, mixing is placed on room temperature 2h.Under agitation dropwise add isopyknic saturated ammonium sulfate, place 1h for 4 DEG C.3000r/min centrifugal half an hour, abandon supernatant, precipitation semi-saturation ammonium sulfate washes twice, and the PBS of precipitation 0.15mol/L dissolves.Loaded in bag filter by above-mentioned solution, dialyse with the PBS of 0.15mol/L, after removing ammonium ion, 10000r/min removes precipitation centrifugal half an hour, and supernatant is drugs antigen enzyme marker.
Embodiment 2 opium class detects the establishment of enzyme linked immunological kit
Set up opium class and detect enzyme linked immunological kit, make it comprise following component:
(1) morphine standard solution, concentration is respectively 0ng/ml, 0.25ng/ml, 0.8ng/ml, 2.4ng/ml, 7ng/ml, 21ng/ml, 1ml/ bottle, with sample diluting liquid dilution hereinafter described;
(2) ELISA Plate of morphine specific antibody is coated with, 1 piece, 96 holes;
(3) morphine enzyme marker, 1 bottle, 13ml;
(4) developer: tetramethyl benzidine phosphate buffer, 1 bottle, 13ml;
(5) concentrated cleaning solution: containing 0.5% tween, 1 ‰ sodium azide, 0.01mol/L PBS, 1 bottle, 50ml, is 10 times of normal working concentration;
(6) stop buffer: 2mol/L sulfuric acid, 1 bottle, 13ml;
(7) sample diluting liquid: 0.01mol/L PBS, 1 bottle, 50ml.
Embodiment 3 amphetamine detects the establishment of enzyme linked immunological kit
Set up amphetamine and detect enzyme linked immunological kit, make it comprise following component:
(1) crystal methamphetamine standard solution, concentration is respectively 0ng/ml, 0.5ng/ml, 1.5ng/ml, 4.5ng/ml, 13.5ng/ml, 40.5ng/ml, 1ml/ bottle, with sample diluting liquid dilution hereinafter described;
(2) ELISA Plate of crystal methamphetamine specific antibody is coated with, 1 piece, 96 holes;
(3) crystal methamphetamine enzyme marker, 1 bottle, 13ml;
(4) developer: tetramethyl benzidine phosphate buffer, 1 bottle, 13ml;
(5) concentrated cleaning solution: containing 0.5% tween, 1 ‰ sodium azide, 0.01mol/L PBS, 1 bottle, 50ml, is 10 times of normal working concentration;
(6) stop buffer: 2mol/L sulfuric acid, 1 bottle, 13ml;
(7) sample diluting liquid: 0.01mol/L PBS, 1 bottle, 50ml.
Embodiment 4 chloramines ketone detects the establishment of enzyme linked immunological kit
Set up chloramines ketone and detect enzyme linked immunological kit, make it comprise following component:
(1) ketamine standard solution, concentration is respectively 0ng/ml, 1.5ng/ml, 3ng/ml, 6ng/ml, 12ng/ml, 24ng/ml, 1ml/ bottle, with sample diluting liquid dilution hereinafter described;
(2) ELISA Plate of ketamine specific antibody is coated with, 1 piece, 96 holes;
(3) ketamine enzyme marker, 1 bottle, 13ml;
(4) developer: tetramethyl benzidine phosphate buffer, 1 bottle, 13ml;
(5) concentrated cleaning solution: containing 0.5% tween, 1 ‰ sodium azide, 0.01mol/L PBS, 1 bottle, 50ml, is 10 times of normal working concentration;
(6) stop buffer: 2mol/L sulfuric acid, 1 bottle, 13ml;
(7) sample diluting liquid: 0.01mol/L PBS, 1 bottle, 50ml.
The detection of opiates in embodiment 5 blood sample
Sample pre-treatments: directly get 20ul blood sample kit and detect.
Kit test method: to be surrounded by morphine specific antibody ELISA Plate micropore in add series standard solution or sample solution, every hole 20ul, then 100ul morphine enzyme marker is added, incubated at room removed liquid in hole after 30 minutes, and every hole adds 300ul cleaning fluid and then goes, and so repeatedly washed plate 3 times, 100ul developer is added after finally patting dry plank with thieving paper, room temperature reaction 15 minutes, uses stop buffer stopped reaction, and microplate reader measures every hole absorbance.
Interpretation of result: the absorbance (B0) of absorbance (B) the division by 0 standard of all standard items or sample, then be multiplied by 100%, obtain percentage absorbance:
Percentage absorbance (%)=(B/B0) × 100%
By in the percentage absorbance of each standard items input semilog system, the concentration of corresponding standard items separately can obtain a typical curve, as shown in Figure 1.Then, the concentration of opiates in corresponding blood sample can be found from typical curve by the percentage absorbance of the opiates in each sample.
The detection of amphetamine material in embodiment 6 hair sample
(1) sample pre-treatments: hair first cleans with methanol solution, cleaning is dried rear scissors and is cut into as far as possible little fragment, take the above-mentioned hair fragment of 20mg, add 2ml methanol solution, 75 DEG C of water-baths are static after two hours is cooled to room temperature, get supernatant 37 DEG C of nitrogen to dry up, residue 160ul sample diluting liquid dilution, on oscillator, vibration is fully dissolved.
(2) kit test method: to be surrounded by crystal methamphetamine specific antibody ELISA Plate micropore in add series standard solution or sample solution, every hole 20ul, then 100ul crystal methamphetamine enzyme marker is added, incubated at room removed liquid in hole after 20 minutes, and every hole adds 300ul cleaning fluid and then goes, and so repeatedly washed plate 3 times, 100ul developer is added after finally patting dry plank with thieving paper, room temperature reaction 8 minutes, uses stop buffer stopped reaction, and microplate reader measures every hole absorbance.
(3) interpretation of result: the absorbance (B0) of absorbance (B) the division by 0 standard of all standard items or sample, then be multiplied by 100%, obtain percentage absorbance:
Percentage absorbance (%)=(B/B0) × 100%
By in the percentage absorbance of each standard items input semilog system, the concentration of corresponding standard items separately can obtain a typical curve, as shown in Figure 2.Then, the concentration of opiates in corresponding blood sample can be found from typical curve by the percentage absorbance of the opiates in each sample.
The detection of chloramines letones in embodiment 7 urine sample
(1) sample pre-treatments: directly get 20ul urine specimen kit and detect.
(2) kit test method: to be surrounded by ketamine specific antibody ELISA Plate micropore in add series standard solution or sample solution, every hole 20ul, then 100ul ketamine enzyme marker is added, incubated at room removed liquid in hole after 30 minutes, and every hole adds 300ul cleaning fluid and then goes, and so repeatedly washed plate 3 times, 100ul developer is added after finally patting dry plank with thieving paper, room temperature reaction 30 minutes, uses stop buffer stopped reaction, and microplate reader measures every hole absorbance.
(3) interpretation of result: the absorbance (B0) of absorbance (B) the division by 0 standard of all standard items or sample, then be multiplied by 100%, obtain percentage absorbance:
Percentage absorbance (%)=(B/B0) × 100%
By in the percentage absorbance of each standard items input semilog system, the concentration of corresponding standard items separately can obtain a typical curve, as shown in Figure 3.Then, the concentration of opiates in corresponding blood sample can be found from typical curve by the percentage absorbance of the opiates in each sample.
The test of embodiment 8 kit precision, accuracy, storage life
(1) kit precision test
Get three batches of (A, B, C) kits respectively and carry out precision test, often criticize kit and extract 3 kits, each kit takes out 10 micropores, measures the absorbance of 4ng/ml standard solution, calculates the coefficient of variation.Measurement result is as shown in table 1, and result shows that the coefficient of variation is between 0.9%-6.5%.
Table 1 kit precision test
(2) kit accuracy test
With the negative urine of people, saliva, blood, hair for matrix, add the series concentration of morphine respectively, be respectively 1.25ng/ml, 5ng/ml, 10ng/ml, as sample.Kit detection and interpretation of result method are with embodiment 5.Each concentration 3 Duplicate Samples, calculate the recovery (%) respectively, result is as shown in table 2.
Table 2 kit accuracy test
(3) kit storage life test
The kit of preparation is kept at 2-8 DEG C respectively, measures the maximum absorbance value (zero standard) of kit, 50% inhibition concentration, drugs interpolation practical measurement value after 12 months all within normal range.Consider in transport and use procedure, have improper preservation condition and occur, placed 7 days by kit, carry out accelerated aging tests under 37 DEG C of preservation conditions, result shows that the kit indices prepared meets the requirements completely.Consider the situation generation that kit is freezing, kit is put into-20 DEG C of refrigerator freezings 7 days, measurement result also shows that the kit indices prepared is completely normal.Can show that kit at least can be preserved more than 12 months at 2-8 DEG C from above result.
Claims (8)
1. an illicit drugs inspection enzyme linked immunological kit, is characterized in that, comprises following material: drugs standard solution, drugs specific antibody; Be coated with the ELISA Plate of drugs specific antibody; Drugs antigen enzyme marker; Developer; Concentrated cleaning solution; Stop buffer and sample diluting liquid.
2. a kind of illicit drugs inspection enzyme linked immunological kit according to claim 1, it is characterized in that, described drugs are morphine, ketamine, hemp, heroin, 6-monoacetylmorphine, codeine or amphetamine compound.
3. a kind of illicit drugs inspection enzyme linked immunological kit according to claim 2, it is characterized in that, described amphetamine compound is amphetamine, crystal methamphetamine, 3,4-methylene-dioxy amphetamine or MDMA.
4. a kind of illicit drugs inspection enzyme linked immunological kit according to claim 1, it is characterized in that, described drugs specific antibody is the conjugate synthesized by carbodiimide method with drugs and carrier protein, and described carrier protein is mouse haemocyanin, bovine serum albumin, thyroprotein, rabbit serum proteins or hemocyanin.
5. a kind of illicit drugs inspection enzyme linked immunological kit according to claim 1, it is characterized in that, the marker enzyme in described drugs antigen enzyme marker is horseradish peroxidase or alkaline phosphatase.
6. a kind of illicit drugs inspection enzyme linked immunological kit according to claim 1, it is characterized in that, described developer is o-phenylenediamine or tetramethyl benzidine.
7. a kind of illicit drugs inspection enzyme linked immunological kit according to claim 1, is characterized in that, described concentrated cleaning solution for containing 0.5% Tween-20, the phosphate buffer of 0.01mol/L; Stop buffer is the sulfuric acid of 1-2mol/L, hydrochloric acid or sodium hydrate buffer solution; Sample diluting liquid is the phosphate buffer of 0.01mol/L.
8. adopt kit described in claim 1 to carry out the method for illicit drugs inspection, it is characterized in that, carry out in accordance with the following steps:
(1) sample pre-treatments
When test sample is urine, saliva, during blood, directly detects; When test sample is hair, first clean with methanol solution, the fragment that rear scissors is cut into 1-10mm is dried in cleaning, take the above-mentioned hair fragment of 2-20mg, add 0.5-2ml methanol solution, 70-75 DEG C of water-bath is static after two hours is cooled to room temperature, get supernatant 37 DEG C of nitrogen to dry up, residue 100-200ul sample diluting liquid dilution, on oscillator, vibration is fully dissolved, to be detected;
(2) in the ELISA Plate being coated with drugs specific antibody, add sample solution or drugs standard solution and drugs antigen enzyme marker solution incubated at room 15-30 minute, the drugs antibody in ELISA Plate competed by drugs in testing sample and the drugs of enzyme labeling, wash plate 3 times, by the unconjugated enzyme marker of washing removing, add developer incubated at room 5-15 minute, stop with stop buffer after colour developing, the absorbance of microplate reader working sample;
(3) in the absorbance that obtains of step (2) and sample, the amount of drugs, in negative relation, compares the concentration that can draw drugs in sample with typical curve.
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| CN109061204A (en) * | 2018-07-30 | 2018-12-21 | 杭州莱和生物技术有限公司 | A kind of kit of fluorescence immunoassay detection hair trace drugs |
| CN110231474A (en) * | 2019-07-08 | 2019-09-13 | 厦门梓蔓生物科技有限公司 | A kind of industrial hemp element efficient detection device and detection method |
| CN111474342A (en) * | 2020-04-20 | 2020-07-31 | 石家庄洹众生物科技有限公司 | Colloidal gold detection card for four-item joint detection of drugs and method for detecting drugs |
| CN111751537A (en) * | 2020-05-21 | 2020-10-09 | 佛山市顺德区公安局 | Test strip, device and method for detecting trace drugs |
| CN112649610A (en) * | 2020-12-09 | 2021-04-13 | 厦门海洋职业技术学院 | Detection kit and detection method for methamphetamine drugs in hair |
| CN112748240A (en) * | 2020-12-24 | 2021-05-04 | 南京北成光生物科技有限公司 | Kit for detecting drugs in hair and detection method thereof |
| CN112946284A (en) * | 2021-01-31 | 2021-06-11 | 古镜科技(深圳)有限公司 | Three-sample-in-one drug fluorescent quintuplet test paper and preparation method thereof |
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| CN111751537A (en) * | 2020-05-21 | 2020-10-09 | 佛山市顺德区公安局 | Test strip, device and method for detecting trace drugs |
| CN112649610A (en) * | 2020-12-09 | 2021-04-13 | 厦门海洋职业技术学院 | Detection kit and detection method for methamphetamine drugs in hair |
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