CN109490546A - Six detection devices of drugs and preparation method thereof - Google Patents
Six detection devices of drugs and preparation method thereof Download PDFInfo
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- CN109490546A CN109490546A CN201811569225.3A CN201811569225A CN109490546A CN 109490546 A CN109490546 A CN 109490546A CN 201811569225 A CN201811569225 A CN 201811569225A CN 109490546 A CN109490546 A CN 109490546A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
Abstract
Six detection devices of drugs of drug species and preparation method thereof in the samples such as saliva can be quickly detected the invention discloses one kind.Six detection devices of the drugs include detection card, test strip and sample-adding funnel.Test strip includes substrate and sample pad, bonding pad, detection film and absorption pad, sample pad is located at the lower section in loading slot and being located at sample-adding funnel, bonding pad is equipped with the monoclonal antibody specific of the coloured microballoon label of six kinds of drugs, the detection line of six kinds of drugs is corresponded respectively on detection film at least six, each detection line is coated with the synthetic antigen of corresponding drugs, and detection line is exposed to observation window.Six kinds of drugs are morphine, meth, ketamine, dimethyl dioxygen amphetamine, tetrahydro-cannabinolic acid and cocaine.Six detection devices of above-mentioned drugs can detect six kinds of common drugs in saliva simultaneously, greatly improve the convenience of illicit drugs inspection in saliva, compared to traditional detection device, detection efficiency is greatly improved.
Description
Technical field
The present invention relates to detection fields, more particularly, to a kind of six detection devices of drugs and preparation method thereof.
Background technique
With the development of the social economy, motor vehicle and driver's quantity persistently increase substantially, drug addict's number in driver
Amount also continues to increase.Consuming illegal drugs can make driver's spirit excited, hallucinate, and judgement be reduced, to the danger of traffic safety
Evil is even more than drunk driving.One, Britain is studies have shown that drive when intoxicated slower than the normal reaction time 12%, and " driving with poison " is more anti-than normal
Slow 21% between seasonable.Drug addict often will appear mirage, and driving ability seriously undermines, and bury for pernicious traffic accident hidden
Suffer from.Driving with poison has seriously injured the life security of people as dangerous driving crime.
Drugs Checkout Gear currently on the market mainly to detect based on urine, exist sampling is complicated, sample is easy to pollute,
The problems such as invasion of privacy；Even if there is the product of individual saliva detections on the market, there is also sampling amount deficiencies, and saliva chromatography process is too
Slowly, the problems such as sensitivity is lower, and the drug species that can once detect are few.
Summary of the invention
Based on this, it is necessary to provide a kind of drugs six detections that can quickly detect the drug species in the samples such as saliva
Device and preparation method thereof.
A kind of six detection devices of drugs, including detection card, test strip and sample-adding funnel；The detection card has
Test chamber, and it is equipped with the loading slot and observation window being connected to the test chamber, the test strip is mounted on the test chamber
Interior, the sample-adding funnel is mounted on the loading slot；The test strip include substrate and set on the substrate and from
One end of the substrate to the sequentially connected sample pad of the other end, bonding pad, detection film and absorption pad, the sample pad is located at institute
The lower section of the sample-adding funnel is stated in loading slot and is located at, the bonding pad is equipped with the spy of the coloured microballoon label of six kinds of drugs
Specific monoclonal antibodies, it is described to detect the detection lines for corresponding respectively to six kinds of drugs on film at least six, each detection
Line is coated with the synthetic antigen of corresponding drugs, and the detection line is exposed to the observation window；Six kinds of drugs are morphine, methyl peace
Fei Taming, ketamine, dimethyl dioxygen amphetamine, tetrahydro-cannabinolic acid and cocaine.
In one of them embodiment, a test strip, detection examination are equipped in each detection card
The bonding pad of paper slip is equipped with the monoclonal antibody specific of six kinds of coloured microballoon labels, and on the detection film of the test strip
With six detection lines；Or
Two test strips are equipped in each detection card, the bonding pad of each test strip is equipped with
The monoclonal antibody specific of three kinds of coloured microballoon labels, and there are corresponding three on the detection film of each test strip
The detection line, drugs of two test strips for detection do not repeat.
In one of them embodiment, the nature controlling line of coating sheep anti-mouse igg antibody is additionally provided on the detection film.
In one of them embodiment, the coloured microballoon is red microsphere.
A kind of production method of six detection devices of drugs, includes the following steps:
Prepare bonding pad and detection film；Wherein, the preparation step of the bonding pad is as follows: six kinds that coloured microballoon is marked
The monoclonal antibody specific solution of drugs is uniformly coated on bonding pad, after drying to obtain the final product；The preparation step of the detection film
It is as follows: the synthetic antigen solution of six kinds of drugs is lined respectively on the detection film, the detection for corresponding to different drugs is formed
Line, after drying to obtain the final product；Six kinds of drugs are morphine, meth, ketamine, dimethyl dioxygen amphetamine, tetrahydro
Cannabinol and cocaine；
Sample pad, bonding pad, detection film and absorption pad are sequentially adhered to the one of substrate from one end of substrate to the other end
On side surface, test strip is formed；
The test strip is mounted in the test chamber of detection card, is located at sample pad in the loading slot of detection card,
And detection line is exposed to the observation window of detection card；
Sample-adding funnel is corresponded to above sample pad and is mounted in the loading slot.
In one of them embodiment, the monoclonal antibody specific solution of six kinds of drugs of the coloured microballoon label
It is to be made by the steps to obtain:
Coloured microballoon is dissolved in MES buffer, the activation of EDC/NHS activator is added；
It washs, be centrifuged after activation, the coloured microballoon of collection is redissolved with borate buffer, the spy of corresponding drugs is then added
Specific monoclonal antibodies are reacted；
Sealer is added after having reacted to be closed；
After closing, washing, centrifugation collect solid and borate buffer and sealer are used to redissolve again, and ultrasound makes coloured micro-
The monoclonal antibody specific of ball label is dispersed in buffer.
In one of them embodiment, the mass ratio of coloured microballoon and monoclonal antibody specific is (0.8~1.2):
0.03；And/or
The sealer is bovine serum albumin(BSA)；And/or
Coloured microballoon label is special in the monoclonal antibody specific solution of six kinds of drugs of the coloured microballoon label
The concentration of property monoclonal antibody is 0.1~1mg/mL.
In one of them embodiment, the synthetic antigen solution is that corresponding synthetic antigen is used phosphate-buffered
Liquid or trehalose diluted to concentration are that 0.3~1mg/mL is obtained.
In one of them embodiment, the production method of six detection devices of drugs further includes making on detection film
It is crossed with sheep anti-mouse igg antibody solution, and dry the step of forming nature controlling line；The sheep anti-mouse igg antibody solution be by
Sheep anti-mouse igg antibody is that 1~3mg/mL is obtained using phosphate buffer or trehalose diluted to concentration.
It is to be made as follows when production forms the test strip in one of them embodiment
Make:
It is corresponding by the synthetic antigen even spread of six kinds of drugs to the bonding pad for being used to make same test strip
Ground crosses the monoclonal antibody specific of the coloured microballoon label of six kinds of drugs to the inspection for being used to make same test strip
The different zones of film are surveyed, the test strip of every of formation has the synthetic antigen and corresponding coloured microballoon of six kinds of drugs
The monoclonal antibody specific of label；Or
By three kinds of drugs synthetic antigen even spreads in six kinds of drugs to the combination for being used to make same test strip
On pad, accordingly, the monoclonal antibody specific that the coloured microballoons of three kinds of drugs marks is crossed to being used to make same inspection
The different zones of the detection film of paper slip are tested, there are three types of the synthetic antigens and phase of drugs for the test strip tool of every of formation
The monoclonal antibody specific for the coloured microballoon label answered, the synthetic antigen of another three kinds of drugs and corresponding coloured microballoon label
Monoclonal antibody specific is formed in another test strip；Correspondingly, subsequent to be put into two test strips together
In the test chamber of one detection card.
Six detection devices of above-mentioned drugs can detect six kinds of common drugs in saliva simultaneously, greatly improve saliva
The convenience of middle illicit drugs inspection, compared to the detection device that tradition can only detect 1~3 kind of drugs, detection efficiency is greatly improved.
Also, six detection devices of the drugs are marked using the higher coloured microballoon (such as red microsphere) of developing sensitivity
Note, relative to tradition using colloidal gold labeled monoclonal antibody and the reagent strip of production, using less antibody starting material, also
Higher sensitivity can be obtained.By experimental verification, the sensitivity that six detection devices of drugs of the invention can detect is respectively:
Morphine is 7.5ng/mL, meth 25ng/mL, ketamine 50ng/mL, dimethyl dioxygen amphetamine are
50ng/mL, tetrahydro-cannabinolic acid 2ng/mL, cocaine 15ng/mL, such detection sensitivity is respectively than urine detection
Sensitivity improves about 10 times, improves about 1 times than other similar saliva detection reagents made using colloid gold label.
Further, six detection devices of the drugs use novel sample-adding structure and sample loading alternative, using smooth sample-adding
Funnel is matched with detection card, when detection, can directly be spat saliva in sample-adding funnel, when saliva flows into sample-adding funnel, saliva
Vacuole foam can float on the top of sample-adding funnel, and most of saliva fluid is sinking to bottom, enable most of saliva fluid suitable
Benefit slides into the test chamber of carrying test strip, and saliva can smoothly touch test strip, while can also be formed certain
It is hydraulic, the Tomography Velocity of saliva is accelerated, detection efficiency is improved.Using above-mentioned this novel sample loading alternative, can avoid passing
Using mouth containing buccal swab in system method, the problem of being detected less than enough saliva amounts, entire detection process are often taken
It is easy to operate, it is easy to use.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of six detection devices of drugs of one embodiment of the invention, and wherein A is top view, and B is to make
Use schematic diagram；
Fig. 2 is the structural schematic diagram of test strip in Fig. 1；
Fig. 3 is the structural schematic diagram of six detection devices of drugs of another embodiment of the present invention, and wherein A is top view, and B is
Use schematic diagram.
Specific embodiment
To facilitate the understanding of the present invention, a more comprehensive description of the invention is given in the following sections with reference to the relevant attached drawings.In attached drawing
Give presently preferred embodiments of the present invention.But the invention can be realized in many different forms, however it is not limited to this paper institute
The embodiment of description.On the contrary, purpose of providing these embodiments is keeps the understanding to the disclosure more thorough
Comprehensively.
It should be noted that it can be directly at another when element is referred to as " being fixed on ", " being set to " another element
On element or there may also be elements placed in the middle.When an element is considered as " connection " another element, it be can be directly
It is connected to another element in succession or may be simultaneously present centering elements.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
Any and all combinations of the listed item of pass.
Incorporated by reference to Fig. 1 and Fig. 2, one embodiment of the invention provides a kind of six detection devices 10 of drugs comprising detection
Card 100, test strip 200 and sample-adding funnel 300.
Detection card 100 has test chamber (not shown), and is equipped with the loading slot 102 and observation window 104 being connected to test chamber.
Test strip 200 is mounted in test chamber, and sample-adding funnel 300 is mounted on loading slot 102.Test strip 200 includes base
Bottom 210 and be located in substrate 210 and from one end of substrate 210 to the sequentially connected sample pad 220 of the other end, bonding pad 230, inspection
Survey film 240 and absorption pad 250.Sample pad 220 is located at the lower section in loading slot 102 and being located at sample-adding funnel 300.
In the present embodiment, bonding pad 230 is equipped with the monoclonal antibody specific of the coloured microballoon label of six kinds of drugs
(such as monoclonal antibody product of Chongqing Tan Sheng Biotechnology Co., Ltd production), can be efficient with the drugs small molecule of detection
It specifically binds.The detection line 242 of six kinds of drugs is corresponded respectively on detection film 240 at least six.Each detection line
242 are coated with corresponding drugs synthetic antigen (such as synthetic antigen of Chongqing Tan Sheng Biotechnology Co., Ltd production).It is a kind of
It is coupled the small molecule of bovine serum albumin(BSA), substance similar with detection drug numerator is more advantageous to and is integrated to detection film 240
On.Detection line 242 is exposed to observation window 104.
Six kinds of drugs of the present invention are morphine (MOP), meth (MET), ketamine (KET), dimethyl
Dioxygen amphetamine (MDMA), tetrahydro-cannabinolic acid (THC), cocaine (COC).
Six detection devices 10 of the drugs can detect six kinds of common drugs in saliva simultaneously, greatly improve saliva
The convenience of middle illicit drugs inspection, compared to the detection device that tradition can only detect 1~3 kind of drugs, detection efficiency is greatly improved.
In Fig. 1 and specific example shown in Fig. 2, a test strip 200 is equipped in each detection card 100.The inspection
The bonding pad 230 for testing paper slip 200 is equipped with the monoclonal antibody specific of six kinds of coloured microballoon labels, and the test strip
There are six detection lines 242 on 200 detection film 240.Thus, which can realize while detect six kinds
The effect of drugs.
In other embodiments, as shown in figure 3, two test strips 200 can also be equipped in each detection card 100.
It is set on the bonding pad 230 of each test strip 200 there are three types of the monoclonal antibody specific of coloured microballoon label, and each detection examination
There is corresponding three detections line 242, drugs of two test strips 200 for detecting are not on the detection film 240 of paper slip 200
It repeats.
Further, in a preferred example, nature controlling line 244 is additionally provided on the detection film 240 of test strip 200.
Nature controlling line 244 is preferably coated with sheep anti-mouse igg antibody.
Further, coloured microballoon used in this application is red microsphere, strong with signal, be easy to observe and show
Beneficial effect, relative to tradition using colloidal gold labeled monoclonal antibody and the reagent strip of production, in the feelings using less antibody starting material
Under condition, moreover it is possible to obtain higher sensitivity.By experimental verification, the detection sensitivity ratio of six detection devices of drugs of the invention
The sensitivity of urine detection improves about 10 times, improves about 1 than other similar saliva detection reagents made using colloid gold label
Times, it can specifically join subsequent experimental section.
Six detection devices 10 of the drugs use novel sample-adding structure and sample loading alternative, using smooth sample-adding funnel
300 100 match with detection card, when detection, can directly spit saliva in sample-adding funnel 300, when saliva flows into sample-adding funnel
When 300, saliva foam can float on the top of sample-adding funnel 300, and most of saliva fluid is sinking to bottom, so that most of saliva
Liquid liquid can be slided into smoothly in the test chamber of carrying test strip 200, and saliva can smoothly touch test strip 200, together
When, can also form certain hydraulic, accelerate the Tomography Velocity of saliva, improve detection efficiency.Added using above-mentioned this novel
Sample loading mode can avoid, using mouth containing buccal swab, often taking in conventional method and ask less than what enough saliva amounts were detected
Topic, entire detection process is easy to operate, is easy to use.
The present invention further additionally provides a kind of production method of six detection devices of drugs comprising following steps:
Step 1: bonding pad and detection film are prepared；Wherein, the preparation step of bonding pad is as follows: coloured microballoon is marked
The monoclonal antibody specific solution of six kinds of drugs is uniformly coated on bonding pad, after drying to obtain the final product；Detect the preparation step of film
It is as follows: the synthetic antigen solution of six kinds of drugs to be lined on detection film respectively, form the detection line for corresponding to different drugs, done
After dry to obtain the final product；Six kinds of drugs be morphine, meth, ketamine, dimethyl dioxygen amphetamine, tetrahydro-cannabinolic acid and
Cocaine；
Step 2: sample pad, bonding pad, detection film and absorption pad are sequentially adhered to base from one end of substrate to the other end
On one side surface at bottom, test strip is formed；
Step 3: test strips be will test and be mounted in the test chamber of detection card, sample pad is made to be located at the loading slot of detection card
In, and detection line is exposed to the observation window of detection card；
Step 4: sample-adding funnel is corresponded to above sample pad and is mounted in loading slot.
In step 1, the monoclonal antibody specific solution of six kinds of drugs of coloured microballoon label is as follows
It is prepared:
The coloured microballoon (such as red microsphere) of 100nm~400nm of Nanjing Egg-based Biotechnology Co., Ltd.'s production is molten
In the MES buffer of 20mM~100mM, preferably pH is 5~7, and the activation of EDC/NHS activator room temperature, the amount of being preferably added to is added
For 5~50 μ L, the time is 10~60min；
It washing, is centrifuged after activation, the coloured microballoon of collection is redissolved with borate buffer, preferred concentration is 5mM~50mM,
PH7~8.5, the monoclonal antibody specific that corresponding drugs are then added are reacted；
Sealer is added after having reacted to be closed, the amount of being preferably added to is 50~100 μ L；
After closing, washing, centrifugation collect solid and borate buffer and sealer are used to redissolve again, be preferably added to concentration
For 5mM~20mM, the amount for restoring original volume is added in pH7~8.5, and ultrasound keeps the specific monoclonal of coloured microballoon label anti-
Body is dispersed in buffer, is kept in dark place in 2~8 DEG C.
Wherein, the mass ratio of coloured microballoon and monoclonal antibody specific is preferably (0.8~1.2): 0.03.Sealer is excellent
Choosing is bovine serum albumin(BSA).It is coloured in the monoclonal antibody specific solution of six kinds of drugs of the coloured microballoon label finally prepared
The concentration of the monoclonal antibody specific of microballoon label is preferably 0.1~1mg/mL.
BioDot point film instrument be can be used but not limited to when coating by corresponding solution even application by pretreated
On bonding pad.It is described pretreatment be first bonding pad be added 0.05%~2.50% Tween-80 or TX-100 solution 10mL~
80mL drying is modified.
In step 1, in a specific example, synthetic antigen solution is that corresponding synthetic antigen is used phosphoric acid
Salt buffer or trehalose diluted to concentration are that 0.3~1mg/mL is obtained.
In a specific example, in step 1, the production method of six detection devices of drugs further includes examining
It surveys on film and is crossed using sheep anti-mouse igg antibody solution, and dry the step of forming nature controlling line.Wherein, sheep anti-mouse igg antibody
Sheep anti-mouse igg antibody is preferably 1~3mg/mL using phosphate buffer or trehalose diluted to concentration by solution
It obtains.
In step 1, it is small that drying condition when production detects film can be but not limited at 37~45 DEG C dry 12~48
When；Drying condition when making bonding pad can be but not limited at 37~45 DEG C 1~2 hour dry.
Further, it is to be made as follows when production forms test strip:
It is corresponding by the synthetic antigen even spread of six kinds of drugs to the bonding pad for being used to make same test strip
Ground crosses the monoclonal antibody specific of the coloured microballoon label of six kinds of drugs to the inspection for being used to make same test strip
The different zones of film are surveyed, every test strip of formation has the synthetic antigen and corresponding coloured microballoon label of six kinds of drugs
Monoclonal antibody specific；Or
By three kinds of drugs synthetic antigen even spreads in six kinds of drugs to the combination for being used to make same test strip
On pad, for example, ketamine, meth, tetrahydro-cannabinolic acid be one group, in addition morphine, dimethyl dioxygen amphetamine,
Cocaine is one group, is reduced because of the similar and inevitable cross reaction of molecular formula.Accordingly, by the coloured micro- of three kinds of drugs
The monoclonal antibody specific of ball label is crossed to the different zones of the detection film for making same test strip, formation
There are three types of the monoclonal antibody specifics of the synthetic antigen of drugs and corresponding coloured microballoon label for every test strip tool, separately
The monoclonal antibody specific of the synthetic antigen of three kinds of drugs and corresponding coloured microballoon label is formed in another Test paper
On item；Correspondingly, in the subsequent test chamber that two test strips are put into same detection card.
The production method of above-mentioned six detection devices of drugs is simple and easy, can be widely used to promote.
The following are specific detection embodiment parts.
Using six detection devices of above-mentioned drugs, detection time 1-3min, than traditional detection method (5-10min),
Such as 1 times of gas chromatography 10-20min, Yao Kuaiyue or more.
Using six detection devices of above-mentioned drugs, there is higher sensitivity to six kinds of common drugs of detection, with normal
Human urine dilutes traditional urine detection limit concentration, such as the traditional detection of morphine is limited to 300ng/mL, ketamine 1000ng/
ML, meth 1000ng/mL, tetrahydro-cannabinolic acid 50ng/mL, cocaine 300ng/mL, dimethyl dioxygen
Amphetamine is 500ng/mL.When being diluted to 10 times, the positive can be still detected.By buying three saliva detection examinations on the market
The product of paper slip, while doubling dilution drugs standard items, while being loaded detection.Wherein detection of the invention limits: morphine is
7.5ng/mL, meth 25ng/mL, ketamine 50ng/mL, dimethyl dioxygen amphetamine are 50ng/mL, four
Hydrogen cannabinol is 2ng/mL, cocaine 15ng/mL, is higher by about 1 times than other saliva testing products.
It is had detected using six detection devices of above-mentioned drugs and is determined as negative 30 person-portion of sample, 6 kinds of drugs are respectively sun
Property each 30 parts of sample, detect coincidence rate 95% or more.Acquire the saliva of 60 healthy populations (no pharmacological dependence phenanthrene is taken drugs individual)
Liquid, wherein the drugs standard items of 30 parts of addition purchases to saliva detects limit concentration, such as ketamine is 100ng/mL, is loaded respectively
Add survey.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of six detection devices of drugs, which is characterized in that including detection card, test strip and sample-adding funnel；It is described
Detection card has test chamber, and is equipped with the loading slot and observation window being connected to the test chamber, and the test strip is mounted on
In the test chamber, the sample-adding funnel is mounted on the loading slot；The test strip includes substrate and is located at described
In substrate and from one end of the substrate to the sequentially connected sample pad of the other end, bonding pad, detection film and absorption pad, the sample
Product pad is located in the loading slot and is located at the lower section of the sample-adding funnel, and the bonding pad is equipped with the coloured micro- of six kinds of drugs
The monoclonal antibody specific of ball label, it is described to detect the detection lines for corresponding respectively to six kinds of drugs on film at least six,
Each detection line is coated with the synthetic antigen of corresponding drugs, and the detection line is exposed to the observation window；Six kinds of drugs are
Morphine, meth, ketamine, dimethyl dioxygen amphetamine, tetrahydro-cannabinolic acid and cocaine.
2. six detection devices of drugs as described in claim 1, which is characterized in that be equipped with an institute in each detection card
Test strip is stated, the bonding pad of the test strip is equipped with the monoclonal antibody specific of six kinds of coloured microballoon labels, and
There are six detection lines on the detection film of the test strip；Or
Be equipped with two test strips in each detection card, set on the bonding pad of each test strip there are three types of
The monoclonal antibody specific of coloured microballoon label, and have described in corresponding three on the detection film of each test strip
Detection line, drugs of two test strips for detection do not repeat.
3. six detection devices of drugs as claimed in claim 1 or 2, which is characterized in that be additionally provided with coating on the detection film
The nature controlling line of sheep anti-mouse igg antibody.
4. six detection devices of drugs as claimed in claim 1 or 2, which is characterized in that the coloured microballoon is red microsphere.
5. a kind of production method of six detection devices of drugs, which comprises the steps of:
Prepare bonding pad and detection film；Wherein, the preparation step of the bonding pad is as follows: six kinds of drugs that coloured microballoon is marked
Monoclonal antibody specific solution be uniformly coated on bonding pad, it is dry after to obtain the final product；The preparation step of the detection film is as follows:
The synthetic antigen solution of six kinds of drugs is lined respectively on the detection film, the detection line for corresponding to different drugs is formed, is done
After dry to obtain the final product；Six kinds of drugs are morphine, meth, ketamine, dimethyl dioxygen amphetamine, tetrahydrocannabinol
Acid and cocaine；
Sample pad, bonding pad, detection film and absorption pad are sequentially adhered to the side table of substrate from one end of substrate to the other end
On face, test strip is formed；
The test strip is mounted in the test chamber of detection card, is located at sample pad in the loading slot of detection card, and examined
Survey line is exposed to the observation window of detection card；
Sample-adding funnel is corresponded to above sample pad and is mounted in the loading slot.
6. the production method of six detection devices of drugs as claimed in claim 5, which is characterized in that the coloured microballoon label
The monoclonal antibody specific solution of six kinds of drugs be to be made by the steps to obtain:
Coloured microballoon is dissolved in MES buffer, the activation of EDC/NHS activator is added；
It washs, be centrifuged after activation, the coloured microballoon of collection is redissolved with borate buffer, the specificity of corresponding drugs is then added
Monoclonal antibody is reacted；
Sealer is added after having reacted to be closed；
After closing, washing, centrifugation collect solid and borate buffer and sealer are used to redissolve again, and ultrasound makes coloured microballoon mark
The monoclonal antibody specific of note is dispersed in buffer.
7. the production method of six detection devices of drugs as claimed in claim 6, which is characterized in that coloured microballoon and specificity
The mass ratio of monoclonal antibody is (0.8~1.2): 0.03；And/or
The sealer is bovine serum albumin(BSA)；And/or
The specificity of coloured microballoon label is single in the monoclonal antibody specific solution of six kinds of drugs of the coloured microballoon label
The concentration of clonal antibody is 0.1~1mg/mL.
8. the production method of six detection devices of drugs as claimed in claim 5, which is characterized in that the synthetic antigen solution
It is to obtain corresponding synthetic antigen for 0.3~1mg/mL to concentration using phosphate buffer or trehalose diluted.
9. the production method of six detection devices of drugs as described in any one of claim 5~8, which is characterized in that also wrap
It includes and is crossed on detection film using sheep anti-mouse igg antibody solution, and dry the step of forming nature controlling line；The sheep anti mouse
It using phosphate buffer or trehalose diluted to concentration is 1~3mg/ that IgG antibody solution, which is by sheep anti-mouse igg antibody,
ML is obtained.
10. the production method of six detection devices of drugs as described in any one of claim 5~8, which is characterized in that making
It is to be made as follows when forming the test strip:
It, accordingly, will by the synthetic antigen even spread of six kinds of drugs to the bonding pad for being used to make same test strip
The monoclonal antibody specific of the coloured microballoon label of six kinds of drugs is crossed to the detection film for making same test strip
Different zones, the test strip of every of formation has the synthetic antigen and corresponding coloured microballoon label of six kinds of drugs
Monoclonal antibody specific；Or
By three kinds of drugs synthetic antigen even spreads in six kinds of drugs to the bonding pad for being used to make same test strip,
Accordingly, the monoclonal antibody specific that the coloured microballoons of three kinds of drugs marks is crossed to being used to make same Test paper
The different zones of the detection film of item, the test strip tool of every of formation is there are three types of the synthetic antigen of drugs and has accordingly
The monoclonal antibody specific of color microballoon label, the specificity of the synthetic antigen of another three kinds of drugs and corresponding coloured microballoon label
Monoclonal antibody is formed in another test strip；Correspondingly, subsequent that two test strips are put into same detection
In the test chamber of card.
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| CN201811569225.3A CN109490546A (en) | 2018-12-21 | 2018-12-21 | Six detection devices of drugs and preparation method thereof |
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| CN201811569225.3A CN109490546A (en) | 2018-12-21 | 2018-12-21 | Six detection devices of drugs and preparation method thereof |
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| CN110058012A (en) * | 2019-04-08 | 2019-07-26 | 石家庄洹众生物科技有限公司 | The fluorescence immunoassay test paper group and its preparation and application of synchronous detection diacetylmorphine, crystal methamphetamine, ketamine and cocaine |
| CN110850095A (en) * | 2019-11-22 | 2020-02-28 | 佛山墨赛生物技术有限公司 | Drug trace detection test strip and preparation method and kit thereof |
| CN111474342A (en) * | 2020-04-20 | 2020-07-31 | 石家庄洹众生物科技有限公司 | Colloidal gold detection card for four-item joint detection of drugs and method for detecting drugs |
| CN112986567A (en) * | 2021-01-27 | 2021-06-18 | 佛山微侦警用科技有限公司 | Reagent component ratio and preparation method of multi-project rapid quantitative drug detection box |
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