CN111467275A - Herbal bacteriostatic hand sanitizer and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of daily chemicals, and particularly relates to a herbal bacteriostatic hand sanitizer and a preparation method thereof. The hand sanitizer takes the composition of piroctone olamine, grapefruit oil and disodium cocoyl glutamate as main antibacterial components, has obvious inhibition effects on staphylococcus aureus and escherichia coli, particularly candida albicans, is safe and non-irritant by being matched with a highly natural skin care agent from plants, can further improve antibacterial activity and blend fragrance, so that the hand sanitizer has lasting special plant fragrance and achieves the effect of moistening skin; and the antibacterial effect has no obvious change after being placed for 90 days under the accelerated stability test investigation condition, the stability is good, and the foaming performance is better.

Description

Herbal bacteriostatic hand sanitizer and preparation method thereof

Technical Field

The invention belongs to the technical field of daily chemicals. More particularly, relates to a herbal bacteriostatic hand sanitizer and a preparation method thereof.

Background

In activities such as daily life, work and the like, both hands are most often in contact with the outside, and the skin of the hands often has a large amount of microorganisms or dirt such as bacteria and fungi, and if the hands are not cleaned, the hands are in contact with nasal cavities and eyes or directly take food for eating, the hands are very easy to infect the microorganisms such as bacteria and fungi, and various diseases are caused. Therefore, the daily chemical hand sanitizer commonly used for cleaning hands is required to have the functions of bacteriostasis, sterilization and the like in addition to the daily required cleaning function so as to achieve the purpose of thorough cleaning. The main antibacterial and bacteriostatic substances of the currently marketed antibacterial hand sanitizer are parachlorometaxylenol, o-phenylphenol, triclocarban and the like, and although the antibacterial and bacteriostatic hand sanitizer has a good antibacterial effect, certain irritation and potential safety hazards also exist.

In order to develop bacteriostatic hand sanitizer with high safety and small irritation, the skilled person turns the research direction to plant bacteriostasis. For example, chinese patent application CN108295005A discloses a botanical antibacterial hand sanitizer, which comprises tea seed cake extract, ginger extract, sophora flower bud extract, gardenia extract, seaweed extract, and plant extract of mint extract, has good antibacterial and bacteriostatic properties, and has the functions of decontamination, moistening and protecting hands; chinese patent application CN104997684A discloses a traditional Chinese medicine antibacterial hand sanitizer, which comprises traditional Chinese medicine extracts of akebia stem ethanol extract, red paeony root ethanol extract, hibiscus flower water extract and acorus calamus ethyl acetate extract, and has good inhibition effects on staphylococcus aureus and escherichia coli. However, the two or the prior hand washing solutions with plant/traditional Chinese medicine extracts as main bacteriostatic components have the problems of small bacteriostatic effect on fungi (such as candida albicans) and poor stability.

Therefore, the herbal bacteriostatic hand sanitizer which is safe, free of stimulation, remarkable in bacterium and fungus inhibition effect, good in stability and rich in bubbles and has good foamability is urgently needed.

Disclosure of Invention

The invention aims to solve the technical problems of large irritation, potential safety hazard, small fungus inhibition effect and poor stability of the conventional chemical antibacterial hand sanitizer, and provides the herbal antibacterial hand sanitizer which is safe, free of stimulation, remarkable in inhibition effect on bacteria and fungi and good in stability.

The invention aims to provide a special bacteriostatic composition for a herbal bacteriostatic hand sanitizer.

The invention also aims to provide the herbal bacteriostatic hand sanitizer which takes the bacteriostatic composition as a main bacteriostatic component.

The invention also aims to provide a preparation method of the herbal bacteriostatic hand sanitizer.

The above purpose of the invention is realized by the following technical scheme:

a bacteriostatic composition special for a herbal bacteriostatic hand sanitizer comprises piroctone olamine, grapefruit oil and disodium cocoyl glutamate.

Furthermore, the mass ratio of the piroctone olamine salt to the grapefruit oil to the disodium cocoyl glutamate is (0.01-0.2) to (0.02-0.5) to (5-10).

The grapefruit oil has unique aromatic odor, contains oxygen-containing compounds such as monoterpene, sesquiterpene, alcohols, aldehydes, ketones, esters, etc., and also has antioxidant and antibacterial effects. However, in application practice, the grapefruit oil has a good bacteriostatic effect on bacteria such as escherichia coli and staphylococcus aureus, but has a poor bacteriostatic effect on fungi such as candida albicans. The piroctone olamine salt is an anti-dandruff agent commonly used in the field of daily chemicals, can obviously reduce the generation of dandruff, has obvious inhibiting effect on fungal infection, is non-toxic and non-irritant, has high safety, and is widely applied to shampoo products. The inventor uses the piroctone olamine salt and the grapefruit in a combined manner, finds that the inhibition effect of the composition on bacteria is not obviously different from that of the composition when the composition is used separately, even the inhibition effect on fungi is reduced to a certain extent, but when the disodium cocoyl glutamate which has no inhibition effect completely is added, the inhibition effect of the composition on the bacteria and the fungi is obviously improved, and the inhibition effect is better under a certain proportioning condition.

Preferably, the mass ratio of the piroctone olamine salt to the grapefruit oil to the disodium cocoyl glutamate is (0.01-0.1): (0.02-0.2): 5-8).

More preferably, the mass ratio of piroctone olamine salt, grapefruit oil and disodium cocoyl glutamate is 0.06:0.1: 7. In practice, the composition has a good antibacterial effect under the condition of the proportioning.

Furthermore, the bacteriostatic composition also comprises a skin conditioner, wherein the skin conditioner is the melaleuca alternifolia leaf oil, the geranium oil, the cherokee rose fruit extract and the philippine flemingia root alcohol extract.

Furthermore, the mass ratio of the piroctone olamine salt, the melaleuca alternifolia leaf oil, the geranium oil, the cherokee rose fruit extract and the philippine flemingia root alcohol extract is (0.01-0.2): (0.01-0.1): (0.05-0.5): 0.05-0.5).

Researches find that the skin conditioner is added on the basis of the composition of piroctone olamine, grapefruit oil and disodium cocoyl glutamate to further improve the bacteriostatic activity of the bacteriostatic composition, blend fragrance and moisten the skin.

Preferably, the mass ratio of the piroctone olamine salt, the melaleuca alternifolia leaf oil, the geranium oil, the cherokee rose fruit extract and the philippine flemingia root alcohol extract is (0.01-0.1): (0.01-0.05): (0.05-0.2).

More preferably, the mass ratio of the piroctone olamine salt to the melaleuca alternifolia leaf oil to the geranium oil to the cherokee rose fruit extract to the philippine flemingia root alcohol extract is 0.06:0.05:0.01:0.18: 0.16. In practice, the composition has good antibacterial effect and fragrance under the condition of the proportioning.

Still further, the bacteriostatic composition further comprises surfactants, the surfactants being cocamidopropyl betaine, lauryl glucoside and sodium coco sulfate. In practice, the addition of sodium cocoyl sulfate can improve the foamability of the product and make the foam rich and fine.

Furthermore, the mass ratio of the piroctone olamine salt, the cocamidopropyl betaine, the lauryl glucoside and the sodium cocoyl sulfate is (0.01-0.2): (5-15): (1-5).

Preferably, the mass ratio of the piroctone olamine salt, the cocamidopropyl betaine, the lauryl glucoside and the sodium cocoyl sulfate is (0.01-0.1): 8-12): 2-5): 1-3.

More preferably, the mass ratio of piroctone olamine, cocamidopropyl betaine, lauryl glucoside and sodium cocoyl sulfate is 0.06:10:3: 2.

Further, the bacteriostasis refers to inhibition of escherichia coli, staphylococcus aureus and candida albicans.

In addition, the invention also provides a herbal bacteriostatic hand sanitizer which takes the bacteriostatic composition as a main bacteriostatic component.

Further, the formulation is as follows:

phase A: 1-5% of sodium cocoanut oil alcohol sulfate and water, wherein the total amount of the sodium cocoanut oil alcohol sulfate is 100%;

phase B: 5-15% of cocamidopropyl betaine, 1-5% of lauryl glucoside and 5-10% of disodium cocoyl glutamate;

and C phase: 0.1-0.5% of lactic acid, 0.1-0.5% of lauroyl arginine ethyl ester HCl, 0.16-1.3% of skin conditioner, 0.01-0.2% of piroctone olamine salt, 0.02-0.5% of grapefruit oil, 0.1-0.5% of phenoxyethanol, 0.1-0.5% of sodium benzoate, 0.1-0.5% of caramel color and 0.02-0.2% of benzophenone-4.

In addition, the invention also provides a preparation method of the hand sanitizer, which comprises the following steps:

s1, accurately weighing the phase A water, then adding the other phase A raw materials, starting stirring, heating to 80-85 ℃, preserving heat for 10-20 min, and cooling;

s2, cooling to 50-65 ℃, sequentially adding the raw materials of the phase B, continuously stirring at the stirring speed of 25-35 rpm until the mixture is completely uniform and has no particles, preserving the heat for 10-20 min, and beginning to cool;

s3, cooling to below 48 ℃, putting the weighed C-phase raw material into an emulsifying pot, vacuumizing and stirring until the C-phase raw material is completely and uniformly dissolved, continuing to stir for 8-20 min at a constant temperature, and then cooling;

s4, cooling to below 40 ℃, adjusting the pH to be within a range of 5-6, adjusting the viscosity to be 1000-2000, and enabling the light yellow to be transparent, uniform and consistent; discharging after the characteristic smell is qualified.

The invention has the following beneficial effects:

the herbal bacteriostatic hand sanitizer takes the composition of piroctone olamine, grapefruit oil and cocoyl disodium glutamate as main bacteriostatic components, has obvious inhibitory effect on staphylococcus aureus and escherichia coli, particularly candida albicans, is safe and non-irritant by being matched with a highly natural skin care agent from plants, can further improve bacteriostatic activity and blend fragrance, so that the herbal bacteriostatic hand sanitizer has lasting special plant fragrance and achieves the effect of moistening skin; and the antibacterial effect has no obvious change after the antibacterial agent is placed for 90 days under the stability test investigation condition, and the stability is good.

Detailed Description

The present invention is further illustrated by the following specific examples, which are not intended to limit the invention in any way. Reagents, methods and apparatus used in the present invention are conventional in the art unless otherwise indicated.

Unless otherwise indicated, reagents and materials used in the following examples are commercially available.

Example 1 component, composition bacteriostasis test

1. The 2min bacteriostatic effect of piroctone olamine, grapefruit oil, melaleuca alternifolia leaf oil, geranium oil, cherokee rose extract, moghania philippinensis alcohol extract or a combination thereof was determined, and the results are shown in table 1.

Table 1 Components, compositions bacteriostatic test results

As can be seen from table 1, grapefruit oil, melaleuca alternifolia leaf oil, geranium oil, cherokee rose fruit extract and mogrol extract all have certain inhibitory effects on bacteria (escherichia coli, staphylococcus aureus), but have weak inhibitory effects on fungi (candida albicans); the piroctone olamine salt has a strong inhibition effect on fungi, but has a small inhibition effect on bacteria; the combination of the piroctone olamine salt and the plant extracts such as the grapefruit oil and the like has no obvious change compared with the bacteriostatic effect when the piroctone olamine salt is used alone, and the bacteriostatic effect of bacteria and fungi can reach a more ideal level only when the piroctone olamine salt, the grapefruit oil and the cocoyl disodium glutamate are combined.

2. Proportioning optimization of antibacterial composition

After the antibacterial combination is obtained, the proportion of the antibacterial combination is optimized. The result shows that the mass ratio of the piroctone olamine salt to the grapefruit oil to the disodium cocoyl glutamate is (0.01-0.2) to (0.02-0.5) to (5-10), and the optimal mass ratio is 0.06 to 0.1 to 7; the mass ratio of the piroctone olamine salt, the melaleuca alternifolia leaf oil, the geranium oil, the cherokee rose fruit extract and the moghania philippinensis alcohol extract is (0.01-0.2): (0.05-0.2): 0.01-0.1): 0.05-0.5), and the best data is 0.06:0.05:0.1:0.18:0.16, which is shown in the table 2.

TABLE 2 formulation optimization of bacteriostatic compositions

As can be seen from table 2, when the amount of piroctone olamine is increased, the effect of inhibiting bacteria is significantly reduced; when the addition amount of the grapefruit oil is increased, the fungus inhibiting effect is obviously reduced; when the amount of disodium cocoyl glutamate is increased, the inhibitory effect on both bacteria and fungi is reduced.

Embodiment 2 herbal bacteriostatic hand sanitizer

The formula of the herbal bacteriostatic hand sanitizer is shown in table 3.

Table 3 example 2 herbal bacteriostatic hand sanitizer formula

The preparation method comprises the following steps:

s1, accurately weighing the phase A water, then adding the other phase A raw materials, starting stirring, heating to 83 ℃, keeping the temperature for 15min, and cooling;

s2, cooling to 60 ℃, sequentially adding the phase B raw materials, continuously stirring at the stirring speed of 30rpm until the mixture is completely uniform and has no particles, preserving the heat for 15min, and beginning to cool;

s3, cooling to below 48 ℃, putting the weighed C-phase raw material into an emulsifying pot, vacuumizing and stirring until the C-phase raw material is completely and uniformly dissolved, continuing to stir for 10min at the constant temperature, and then cooling;

s4, cooling to below 40 ℃, adjusting the pH to be within a range of 5-6, adjusting the viscosity to be 1000-2000, and enabling the light yellow to be transparent, uniform and consistent; discharging after the characteristic smell is qualified.

Embodiment 3 herbal bacteriostatic hand sanitizer

The formula of the herbal bacteriostatic hand sanitizer is shown in table 4, and the preparation method refers to example 2.

Table 4 example 3 herbal bacteriostatic hand sanitizer formula

Embodiment 4 herbal bacteriostatic hand sanitizer

The formula of the herbal bacteriostatic hand sanitizer is shown in table 5, and the preparation method refers to example 2.

Table 5 example 4 herbal bacteriostatic hand sanitizer formula

Embodiment 5 herbal bacteriostatic hand sanitizer

The difference from example 2 is that example 5 does not add a skin conditioning agent and the remaining parameters and operation refer to example 2.

Comparative example 1 herbal antibacterial hand sanitizer

Except for example 2 that comparative example 1 does not add piroctone olamine salt, the rest of the parameters and operation refer to example 2.

Comparative example 2 herbal antibacterial hand sanitizer

The difference from example 2 is that comparative example 2 does not add grapefruit oil, and the remaining parameters and operation refer to example 2.

Comparative example 3 herbal antibacterial hand sanitizer

The difference from example 2 is that in comparative example 3, no disodium cocoyl glutamate was added, and the rest of the parameters and operation refer to example 2.

Comparative example 4 herbal antibacterial hand sanitizer

The difference from example 2 is that comparative example 4 does not add sodium cocoyl sulfate, and the rest of the parameters and the operation refer to example 2.

Test example 1 test of bacteriostatic effect of hand sanitizer

The antibacterial effect of the hand sanitizer prepared in the examples 2-4 and the comparative examples 1-3 is tested by referring to appendix C of GB 15979-.

TABLE 6 example 2 bacteriostatic rate of hand sanitizer at different times

As can be seen from Table 6, the hand sanitizer in example 2 of the present invention has a bacteriostatic rate of over 95% for Candida albicans, Staphylococcus aureus and Escherichia coli after being applied for 2min, and the bacteriostatic effect meets the requirement of GB 15979-supplement 2002 appendix C C4.2.2 of hygienic Standard for Disposable sanitary articles.

TABLE 7 antibacterial Rate of the liquid soap over different periods of time

As can be seen from Table 7, the hand sanitizer disclosed in the embodiment 2-4 of the invention has significant inhibitory effects on Candida albicans, Staphylococcus aureus and Escherichia coli, and the bacteriostatic effect of 2min can reach more than 99%; compared with the comparative examples 1-3, the piroctone olamine salt, the grapefruit oil and the cocoyl disodium glutamate are respectively removed, so that the bacteriostatic effect is obviously reduced.

Test example 2 hand sanitizer index measurement

Indexes such as sense, physicochemical, and microorganism were measured for the hand sanitizers of examples 2 to 4, and the detection indexes are shown in table 8.

TABLE 8 index of hand sanitizer

Experimental results show that the hand sanitizer prepared in the embodiment 2-4 completely meets the standard requirements.

Test example 3 determination of bacteriostatic stability of hand sanitizer

Taking the liquid soap of example 2 as an example, after the liquid soap samples are alternately placed at (-18 +/-1) DEG C and (45 +/-1) DEG C for 1, 30, 60 and 90 days at intervals in a constant temperature box with a relative humidity of 80%, the conditions of color change, precipitation, layering and the like of the samples are observed, the average bacteriostasis rate of the samples on the candida albicans is detected, and the bacteriostasis stability results of example 2 are shown in Table 9.

Table 9 example 2 determination of bacteriostatic stability of hand sanitizer

Note: before stabilization, the lotion is in the original shape after stabilization; the negative control was established.

As can be seen from table 9, the hand sanitizer stability test in example 2 of the present invention shows that the antibacterial effect does not change significantly after being stored for 90 days, and still maintains a good antibacterial effect and pleasant fragrance, and the effective period of the product can be determined to be 2 years according to the specification of "disinfection specification".

Test example 4 hand sanitizer skin irritation assay

The skin irritation of the hand sanitizer of example 2 was measured by referring to the skin irritation test of disinfection specifications (2017), and the results are shown in table 10.

TABLE 10 skin irritation results of hand sanitizers

According to the skin irritation intensity grading standard in disinfection technical specification (2017 edition), the test result of the acute intact skin irritation of the liquid soap disclosed by the invention to rabbits is as follows: has no irritation, and meets the requirements of disinfection technical Specifications (2017 edition).

Test example 5 measurement of foaming Effect of hand cleanser

The lathering performance of the hand lotions of examples 2-4 and comparative example 4 was measured using a lathering tester (Sita, Germany, Signal R-2000). The lathering performance of the samples was measured by adding 250m L of the solution to be tested to a vector cup, stirring the solution at 1000rpm for 60 seconds, and recording the volume of lather generated at that time.

TABLE 11 determination of foaming Effect of hand washing solutions

As can be seen from table 11, the hand sanitizer prepared in embodiments 2 to 4 of the present invention has abundant foam amount, strong foaming ability, and fine and stable foam.

Test example 6 fragrance retention test of liquid soap

A sensory evaluation method is adopted, 60 testers are randomly arranged, and the hand sanitizer prepared in the embodiment 2-4 and 5 is scored; the evaluation method is that the hand sanitizer is dipped on the fragrance smelling strip, according to the standard that the fragrance retention time is more than or equal to 0 and t is less than 1h and 0min, the fragrance retention time is more than or equal to 1h and t is less than 1h and 1-3 min, the fragrance retention time is more than or equal to 1h and t is less than or equal to 4h and 4-6 min, the fragrance retention time is more than or equal to 7h and less than or equal to 10h and 9 min, the fragrance retention time is more than or equal to 10h and 10min, each hand sanitizer is tested for three times, the average value of the three times. The results of the fragrance residence time test are shown in Table 12.

TABLE 12 fragrance test determination of hand sanitizer

Group of	For the first time	For the second time	The third time	Average score
					Example 2	10	10	10	10
Example 3	10	9	9	9.3
					Example 4	10	9	9	9.3
Example 5	6	3	1	3.3

The fragrance of the liquid soap in the fragrance retention experiment of the embodiment 5 is monotonous, no special pleasant fragrance of plants exists, and the fragrance retention time after use is shorter compared with the liquid soaps of the embodiments 2 to 4.

Test example 7 moisturizing effect test of liquid soap

1. Test materials: the hand sanitizer prepared in examples 2 to 5 and comparative example 2 was a commercial hand sanitizer.

1. Test subjects: volunteers between the ages of 20-45.

3. The test method comprises the following steps:

cleaning both hands with 1ml of hand-washing solution or commercial hand-washing solution and clean water respectively at room temperature of 25 deg.C and relative humidity of 50%, wiping water drops, and measuring skin on the back of the hand of the subject with digital skin moisture tester for 0.5, 1, 2h before and after washing to obtain skin moisture content.

4. And (3) test results: see table 13.

TABLE 13 hand sanitizer moisturizing effectiveness test

As can be seen from table 13, after the hand sanitizer prepared in embodiments 2 to 4 of the present invention is used for washing hands, the moisture content of the back of the hand gradually recovers to a normal level within 2 hours, and the hand sanitizer has a better moisturizing effect compared with commercially available hand sanitizers; in example 5 (without adding plant conditioner), the moisturizing effect is basically not achieved, and the special pleasant fragrance of plants is not generated; the hand sanitizer prepared in comparative example 2 (without grapefruit oil) also had significantly reduced moisturizing effect.

The above embodiments are preferred embodiments of the present invention, but the present invention is not limited to the above embodiments, and any other changes, modifications, substitutions, combinations, and simplifications which do not depart from the spirit and principle of the present invention should be construed as equivalents thereof, and all such changes, modifications, substitutions, combinations, and simplifications are intended to be included in the scope of the present invention.

Claims (10)

1. The antibacterial composition special for the herbal antibacterial hand sanitizer is characterized by comprising piroctone olamine, grapefruit oil and disodium cocoyl glutamate.

2. The bacteriostatic composition according to claim 1, wherein the mass ratio of the piroctone olamine salt, the grapefruit oil and the disodium cocoyl glutamate is (0.01-0.2): (0.02-0.5): (5-10); preferably, the mass ratio of the piroctone olamine salt, the grapefruit oil and the disodium cocoyl glutamate is 0.06:0.1: 7.

3. Bacteriostatic composition according to claim 1 or 2, wherein the skin conditioning agent is melaleuca alternifolia leaf oil, geranium oil, cherokee rose fruit extract or mogrol extract.

4. The bacteriostatic composition according to claim 3, wherein the mass ratio of the piroctone olamine salt, the melaleuca alternifolia leaf oil, the pelargonium roseum oil, the cherokee rose fruit extract and the mogrol extract is (0.01-0.2): 0.05-0.2): 0.01-0.1): 0.05-0.5.

5. Bacteriostatic composition according to claim 1 or 2, wherein the bacteriostatic composition further comprises surfactants, the surfactants being cocamidopropyl betaine, lauryl glucoside and sodium coco sulfate.

6. The bacteriostatic composition according to claim 5, wherein the mass ratio of the piroctone olamine salt, the cocamidopropyl betaine, the lauryl glucoside and the sodium cocoyl sulfate is (0.01-0.2): (5-15): (1-5): 1-5).

7. Bacteriostatic composition according to claim 1 or 2, wherein the bacteriostatic is escherichia coli, staphylococcus aureus and candida albicans.

8. A herbal bacteriostatic hand sanitizer characterized by taking the bacteriostatic composition as claimed in any one of claims 1 to 7 as a main bacteriostatic component.

9. The hand sanitizer of claim 8, wherein the formulation is as follows:

phase A: 1-5% of sodium cocoanut oil alcohol sulfate and water, wherein the total amount of the sodium cocoanut oil alcohol sulfate is 100%;

phase B: 5-15% of cocamidopropyl betaine, 1-5% of lauryl glucoside and 5-10% of disodium cocoyl glutamate;

and C phase: 0.1-0.5% of lactic acid, 0.1-0.5% of lauroyl arginine ethyl ester HCl, 0.16-1.3% of skin conditioner, 0.01-0.2% of piroctone olamine salt, 0.02-0.5% of grapefruit oil, 0.1-0.5% of phenoxyethanol, 0.1-0.5% of sodium benzoate, 0.1-0.5% of caramel color and 0.02-0.2% of benzophenone-4.

10. A method of preparing a hand sanitizer according to claim 9 comprising the steps of:

s1, accurately weighing the phase A water, then adding the other phase A raw materials, starting stirring, heating to 80-85 ℃, preserving heat for 10-20 min, and cooling;

s2, cooling to 50-65 ℃, sequentially adding the raw materials of the phase B, continuously stirring at the stirring speed of 25-35 rpm until the mixture is completely uniform and has no particles, preserving the heat for 10-20 min, and beginning to cool;

s3, cooling to below 48 ℃, putting the weighed C-phase raw material into an emulsifying pot, vacuumizing and stirring until the C-phase raw material is completely and uniformly dissolved, continuing to stir for 8-20 min at a constant temperature, and then cooling;

s4, cooling to below 40 ℃, adjusting the pH to be within a range of 5-6, adjusting the viscosity to be 1000-2000, and enabling the light yellow to be transparent, uniform and consistent; discharging after the characteristic smell is qualified.