CN111466396A

CN111466396A - Insecticidal composition and preparation and application thereof

Info

Publication number: CN111466396A
Application number: CN202010429939.5A
Authority: CN
Inventors: 唐兴敏; 柴方堃; 贾成国
Original assignee: Hebei Veyong Bio Chemical Co ltd
Current assignee: Hebei Veyong Bio Chemical Co ltd
Priority date: 2020-05-20
Filing date: 2020-05-20
Publication date: 2020-07-31
Anticipated expiration: 2040-05-20
Also published as: CN111466396B

Abstract

The invention relates to the technical field of pesticide preparation, and particularly discloses an insecticidal composition, and a preparation and application thereof. The invention provides an insecticidal composition, which selects emamectin B_2aBenzoate, pleocidin and stevioside are compounded, wherein emamectin B_2aThe benzoate and the spinosad have synergistic effect, plant extract stevioside is further added as a synergist, and the synergist and the original medicine emamectin benzoate B_2aThe benzoate and the pleocidin generate synergistic effect, and simultaneously, the specific structure and the sweet taste of the stevioside can be used for introducingLuring the feeding behavior of pests and further improving the pesticide effect. The insecticidal composition provided by the invention has the advantages that the prevention and control effects on thrips pests are obviously improved by selecting raw materials with different action mechanisms for compounding, the effect duration is long, the compatibility with the environment is good, the pests are not easy to generate drug resistance after long-term use, and the popularization value is very high.

Description

Insecticidal composition and preparation and application thereof

Technical Field

The invention relates to the technical field of pesticide preparation, and particularly relates to an insecticidal composition, and a preparation and application thereof.

Background

Thrips are common pests on crops such as vegetables, melons, rice and the like, thrips occur all the year round, spring, summer and autumn mainly occur in open lands, winter mainly occurs in greenhouses, adults and nymphs file and suck young tissue (branches, tips, leaves, flowers, fruits and the like) juice of plants, damaged tender leaves and tips become hard, curl and wither, the plants grow slowly, and internodes are shortened; young fruits (such as eggplants, cucumbers, watermelons and the like) are hardened after being damaged, and fruit drop is caused in severe cases, so that the yield and the quality are seriously influenced. The thrips pests have tiny body types, are usually hidden in plant buds, are difficult to find, have strong reproductive capacity, can be massively erupted into pests in a short time, and are mainly chemically controlled in agricultural production. For a long time, due to the unscientific and reasonable use of chemical pesticides, the pests of thrips have serious resistance, and the effective prevention and control of thrips are difficult to carry out.

Methylamino avermectin B_2aThe benzoate is a macrolide insecticide, has a broad insecticidal spectrum, has high-efficiency killing activity on pests such as lepidoptera, coleoptera, hemiptera, diptera, homoptera, thysanoptera, mites and the like, has a poor control effect on piercing-sucking mouthpart insects, and has a short duration. Therefore, the research and the design of the pollution-free biological pesticide with good killing activity to the thrips pests and long lasting effect have very important significance.

Disclosure of Invention

Aiming at the problems of non-ideal effect and poor persistence of the insecticidal composition on the thrips pests in the prior art, the invention provides the insecticidal composition and a preparation and an application thereof.

In order to solve the technical problems, the technical scheme provided by the invention is as follows:

an insecticidal composition contains methylamino abamectin B as effective component_2aBenzoate and spinosad, and the synergist comprises stevioside.

In the prior art, the insecticidal composition provided by the invention selects emamectin benzoate B_2aBenzoate, pleocidin and stevioside are compounded as main components, wherein emamectin B_2aBoth benzoate and spinosad haveThe synergistic effect is achieved by further adding plant extract stevioside as a synergist and the original medicine emamectin benzoate B_2aThe benzoate and the pleocidin generate a synergistic effect, and meanwhile, the specific structure and the sweet taste of the stevioside can attract the feeding behavior of pests, so that the pesticide effect is further improved. The insecticidal composition provided by the invention has the advantages that the prevention and control effects on thrips pests are obviously improved by selecting raw materials with different action mechanisms for compounding, the effect duration is long, the compatibility with the environment is good, the pests are not easy to generate drug resistance after long-term use, and the popularization value is very high.

Methylamino avermectin B_2aThe benzoate is a novel high-efficiency semi-synthetic antibiotic pesticide synthesized from a fermentation product abamectin B1, has the characteristics of biological pesticides of ultra-high efficiency, low toxicity (preparation is nearly nontoxic), low residue, no public nuisance and the like, is widely used for controlling various pests on crops such as vegetables, fruit trees, cotton and the like, has wide insecticidal spectrum, has high-efficiency killing activity on pests such as lepidoptera, coleoptera, hemiptera, diptera, homoptera, thysanoptera, mites and the like, is effective on underground pests such as mole cricket, root maggot and root aphid, has special effect on the pests, but has unsatisfactory effect on piercing-sucking device insects, and has short duration.

Spinosad, also known as spinosad, is a macrolide pollution-free high-efficiency biopesticide extracted from spinosyn fermentation liquor, has insecticidal activity far exceeding that of organic phosphorus, carbamate, cyclopentadiene and other insecticides, can effectively control pests including lepidoptera, diptera and thysanoptera pests, and simultaneously has certain poisoning effect on certain specific types of pests of coleoptera, orthoptera, hymenoptera, isoptera, siphonaptera, dermaptera and rodentia, but has unsatisfactory control effect on piercing-sucking mouthpart insects and mites.

Stevioside, a novel sweetener extracted from stevia rebaudiana Bertoni of Compositae, has the functional activities of lowering blood pressure, reducing blood fat, resisting tumor, regulating immunity and the like, is widely applied to various foods, beverages, medicines and daily chemical industries, but has not been reported to be applied to the field of pesticides.

Preferably, the first and second liquid crystal materials are,the emamectin B_2aThe mass ratio of the benzoate to the spinosad to the stevioside is 1-40:1-40: 0.1-1.

More preferably, the emamectin B_2aThe mass ratio of the benzoate to the spinosad to the stevioside is 1-10:1-10: 0.4-0.6.

In the case of the preferred mass ratio, emamectin B_2aThe benzoate, the pleocidin and the stevioside are combined for use, so that the synergistic effect is very obvious, the excellent control effect on the thrips pests is realized, and the lasting period can be obviously prolonged.

The invention also provides an insecticide, which comprises the pesticide composition.

Preferably, the pesticide also comprises an auxiliary agent, and the emamectin benzoate B_2aThe total mass of benzoate, spinosad and stevioside accounts for 2.1-81 wt% of the pesticide.

The preferable content is favorable for obtaining higher control effect on the premise of less pesticide dosage.

Preferably, the auxiliary agent comprises at least one of a cosolvent, a dispersing agent, a wetting agent, a defoaming agent, a disintegrating agent, a thickening agent or a filler.

In order to prepare the insecticidal composition into various formulations, a plurality of auxiliary agents are required to be added, and the cosolvent, the dispersant, the wetting agent, the defoamer, the disintegrant, the thickener or the filler are all commonly used in the field of pesticides, and different auxiliary agents can be selected according to different formulations, and specific components and dosage can be determined through routine experiments according to formulation requirements.

Optionally, the pesticide is in the form of microcapsule suspension, suspension seed coating, emulsion in water, microemulsion, emulsifiable concentrate, wettable powder, water dispersible granules or granules.

The preparation can be prepared by selecting conventional additives in the field of pesticides according to a conventional preparation process. The pesticide composition is prepared into various dosage forms, is convenient to use, and meets different pesticide prevention and treatment requirements.

As used hereinThe insecticidal composition can be prepared into a microcapsule suspending agent, and the microcapsule suspending agent comprises the following raw material components in percentage by mass: methylamino avermectin B_2a1-40% of benzoate, 1-40% of spinosad, 1-40% of solvent, 1-10% of wall material, 0.5-8% of curing agent, 1-10% of emulsifier, 1-10% of dispersant, 0.1-2% of thickener, 1-4% of antifreeze, 0.1-0.5% of preservative, 0.1-0.5% of defoamer, 0.1-1% of stevioside and the balance of water to 100%.

Wherein the solvent is at least one of sec-butyl acetate, dimethyl carbonate, N-dimethylformamide, N-methyl pyrrolidone, vegetable oil, mineral oil, methyl oleate, solvent oil and pine-based oil; the wall material is polyisocyanate, aliphatic isocyanate or urea; the curing agent is at least one of glycerol, 1, 2-propylene glycol, 1, 4-butanediol, diethylene glycol, polyethylene glycol 200, polyethylene glycol 400, dimethylamine, isopropylamine, ethylenediamine, triethylamine and triethanolamine; the emulsifier is at least one of sodium lignosulfonate, sodium dodecyl sulfate, sodium dodecyl benzene sulfonate, fatty alcohol-polyoxyethylene ether, sodium lauryl sulfate, polyvinyl alcohol, polycarboxylate, naphthalene sulfonate, castor oil polyoxyethylene ether and sorbitol monolaurate; the dispersing agent is at least one of sodium dodecyl benzene sulfonate, sodium lignosulfonate, nekal, desugarized sodium lignosulfonate, sodium succinate sulfonate, nonylphenol polyoxyethylene ether and fatty alcohol polyoxyethylene ether; the thickening agent is at least one of magnesium aluminum silicate, xanthan gum, polyethylene glycol, polyvinyl alcohol, sodium carboxymethylcellulose, chitosan, bentonite and white carbon black; the antifreezing agent is at least one of ethylene glycol, 1, 2-propylene glycol, glycerol, urea and isopropanol; the preservative is at least one of sodium benzoate, isothiazolinone, benzisothiazolinone, potassium sorbate and quaternary ammonium salt; the defoaming agent is an organic silicon defoaming agent or a polyether defoaming agent.

The preparation of the microcapsule suspending agent from the insecticidal composition can improve the stability of active ingredients, control the release of medicaments, prolong the lasting period, improve the utilization rate of pesticides and reduce the harm to operators.

The invention also provides application of the insecticidal composition in prevention and treatment of thrips pests.

The invention also provides application of the pesticide in prevention and treatment of thrips pests.

The insecticidal composition provided by the invention can be provided in the form of a finished product of a composite preparation, or each component can be provided in the form of a separate preparation and is mixed uniformly before use.

The nematicidal composition or pesticide is applied by dilution and spraying, and the application amount and dilution ratio of the nematicidal composition or pesticide are changed along with the change of biological characteristics or environmental conditions of the control object.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

In order to better illustrate the invention, the following examples are given by way of further illustration.

Example 1

This example provides an insecticidal suspension:

according to methylamino avermectin B_2aThe mass percentage of benzoate, pleocidin and stevioside is 40:1: 0.5. Taking methylamino emamectin B_2a40% of benzoate, 1.0% of spinosad, 2.0% of block polyether, 3.5% of alkylphenol polyoxyethylene ether phosphate, 0.3% of xanthan gum, 5.0% of ethylene glycol, 0.2% of magnesium aluminum silicate, 0.5% of stevioside and deionized water to make up to 100%. The components are uniformly mixed and ground by a grinding machine to obtain the insecticidal suspending agent.

Example 2

This example provides an insecticidal microemulsion:

according to methylamino avermectin B_2aThe mass percentage of benzoate, pleocidin and stevioside is 33:2: 0.1. Taking methylamino AVitinomycin B_2a33.0% of benzoate, 2.0% of spinosad, 10.0% of phenethyl phenol polyoxyethylene ether phosphate, 10.0% of nonylphenol polyoxyethylene ether, 25.0% of sec-butyl acetate, 5.0% of N, N-dimethylformamide, 0.1% of stevioside and deionized water to make up to 100%.

Mixing methylamino emamectin B_2aUniformly mixing benzoate, spinosad, sec-butyl acetate, N-dimethylformamide, nonylphenol polyoxyethylene ether and phenethyl phenol polyoxyethylene ether phosphate to obtain an oil phase; dissolving stevioside in deionized water to obtain a water phase; and adding the water phase into the oil phase under the condition of stirring, and uniformly mixing to obtain the insecticidal microemulsion.

Example 3

This example provides a pesticidal microcapsule suspension:

according to methylamino avermectin B_2aThe mass percentage of benzoate, pleocidin and stevioside is 10:10: 0.8. Taking 10.0% of methylamino abamectin B2a benzoate, 10.0% of spinosad, 2.0% of naphthalenesulfonate, 4.0% of nonylphenol polyoxyethylene ether, 20.0% of sec-butyl acetate, 8.0% of solvent oil, 0.3% of organic silicon, 0.3% of xanthan gum, 1.0% of triethanolamine, 4.0% of polyisocyanate, 2.0% of ethylene glycol, 0.2% of sodium benzoate, 0.8% of stevioside and deionized water to complement to 100%.

The preparation process comprises the following steps:

dissolving emamectin benzoate B2a and spinosad with sec-butyl acetate and solvent oil respectively, adding polyisocyanate and naphthalenesulfonate, and uniformly stirring to obtain an oil phase;

adding nonylphenol polyoxyethylene ether, sodium benzoate and organic silicon into deionized water to obtain a water phase;

step three, adding the oil phase into the water phase under stirring to obtain an oil-in-water emulsion;

and step four, adding triethanolamine into the oil-in-water emulsion under stirring, stirring for 1-2 hours at 55-65 ℃, adding ethylene glycol, xanthan gum and stevioside, and mixing and stirring to obtain the insecticidal microcapsule suspending agent.

Example 4

The embodiment provides an insecticidal water dispersible granule:

according to methylamino avermectin B_2aThe mass percentage of benzoate, pleocidin and stevioside is 4:21: 1.0. Taking methylamino abamectin B_2a4.0% of benzoate, 21.0% of spinosad, 7.0% of polyvinylpyrrolidone, 2.5% of sodium dodecyl benzene sulfonate, 7.0% of polycarboxylate, 12.0% of corn starch, 0.2% of magnesium aluminum silicate, 1.0% of stevioside and 100% of kaolin.

The preparation process comprises the following steps: the components are uniformly mixed, are crushed into wettable powder through airflow, are added with water to be mixed, extruded and granulated, and are dried and screened to obtain the water dispersible granule.

Example 5

This example provides a pesticidal microcapsule suspension:

according to methylamino avermectin B_2aThe mass percentage of benzoate, pleocidin and stevioside is 1:40: 0.6. Taking methylamino abamectin B_2a1.0% of benzoate, 40.0% of spinosad, 2.5% of sodium dodecyl benzene sulfonate, 2.5% of nonylphenol polyoxyethylene ether, 18.0% of solvent oil, 0.3% of organic silicon, 0.5% of chitosan, 2.5% of isopropylamine, 5.0% of aliphatic isocyanate, 2.0% of ethylene glycol, 0.2% of sodium benzoate, 0.6% of stevioside and deionized water to complement to 100%.

The preparation process comprises the following steps:

dissolving emamectin benzoate B2a and spinosad in solvent oil, adding aliphatic isocyanate and sodium dodecyl benzene sulfonate, and uniformly stirring to obtain an oil phase;

adding nonylphenol polyoxyethylene ether, chitosan, sodium benzoate and organic silicon into deionized water to obtain a water phase;

and step four, adding isopropylamine into the oil-in-water emulsion under stirring, stirring for 1-2h at 55-65 ℃, adding ethylene glycol and stevioside, and mixing and stirring to obtain the insecticidal microcapsule suspending agent.

The insecticidal composition provided by the invention can be prepared into other formulations such as emulsion in water, wettable powder, missible oil or granules by selecting conventional auxiliary materials in the field of pesticides according to a conventional process, and other formulations with the same effective component content can achieve the insecticidal effect basically the same as the formulations in examples 1-5.

Comparative examples 1 to 5

Comparative examples 1 to 5 the same raw materials and preparation methods as those of examples 1 to 5 were used, except that the steviol glycosides of examples 1 to 5 were replaced with the same amount of deionized water to prepare the corresponding insecticides.

Comparative example 6

This comparative example used the same raw materials and preparation methods as example 4, except that spinosad was replaced with emamectin B in equal weight_2aBenzoate to obtain the insecticidal water dispersible granule.

Comparative example 7

This comparative example uses the same starting materials and preparation as in example 4, except that emamectin B is added_2aAnd replacing benzoate with spinosad with equal weight to obtain the insecticidal water dispersible granule.

Comparative example 8

This comparative example uses the same starting materials and preparation as in example 4, except that amino abamectin B is added_2aAnd replacing benzoate and spinosad with stevioside with equal weight to obtain the insecticidal water dispersible granule.

Comparative example 9

The comparative example adopts the same raw materials and preparation method as the example 4, except that the spinosad is replaced by sulfoxaflor with the same weight to obtain the insecticidal water dispersible granule.

Comparative example 10

The comparative example adopts the same raw materials and preparation method as those in example 4, except that the spinosad is replaced by sulfoxaflor with equal weight, and the stevioside is replaced by deionized water with equal weight, so that the insecticidal water dispersible granule is obtained.

Comparative example 11

The comparative example adopts the same raw materials and preparation method as those in example 4, except that stevioside is replaced by astragalus polysaccharide with the same weight to obtain the insecticidal water dispersible granule.

Comparative example 12

This comparative example used the same raw materials and preparation methods as example 4, except that steviol glycosides and spinosad were replaced with emamectin benzoate B of equal weight_2aBenzoate to obtain the insecticidal water dispersible granule.

Comparative example 13

This comparative example uses the same raw materials and preparation method as example 4, except that steviol glycoside and emamectin B are added_2aAnd replacing benzoate with spinosad with equal weight to obtain the insecticidal water dispersible granule.

In order to determine the insecticidal effect of the product, a field efficacy test is carried out.

Combined virulence assay

Amino abamectin B_2aThe toxicity of benzoate and spinosad technical products on the peashrub thrips is respectively measured, the single-dose and compound-dose experimental liquid medicines are respectively diluted into five series concentrations and are respectively placed in a beaker for standby, the method of soaking leaves firstly and then inoculating insects is adopted, the leaves which are not contacted with any medicament and have consistent sizes are soaked in the prepared liquid medicine for 5s and then taken out, the leaves are naturally aired, the leaves are placed in an insect breeding box, then the leaves are inoculated with test larvae and are bred at the temperature of 25 ℃, the number of the used test insects is 50 for each repetition, a blank control is simultaneously arranged, the number of dead insects is checked for 72h, the death rate and the corrected death rate are calculated, a toxicity regression equation is obtained, an L C50 value is calculated, the insecticidal activity of each medicament is evaluated, and the co-toxicity coefficient (CTC value) of the mixture is calculated according to the Yinfei method, the calculation formula of the co-toxicity coefficient is as follows:

observed virulence index (ATI) ═ standard (L C50/test agent L C50) × 100

Theoretical virulence index (TTI) ═ percentage of A in the A agent ATI × mixture + percentage of B in the B agent ATI × mixture

Cotoxicity coefficient (CTC) ([ measured toxicity index (ATI)/Theoretical Toxicity Index (TTI)) × 100

When the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is more than 80 and less than 120, the composition shows additive action, and when the CTC is more than or equal to 120, the composition shows synergistic action.

TABLE 1 Combined virulence determination of the individual and combination Agents against Cynara cardunculus

The combined virulence test results in the table above show that emamectin B_2aThe compound of the benzoate and the spinosad has a synergistic effect on the co-toxicity coefficient CTC of the joint toxicity determination of the pea thrips in the range of the ratio of 40:1-1:40, and the co-toxicity coefficient CTC is more than 130. Wherein the emamectin B_2aWhen the weight ratio of the benzoate to the spinosad is 10:1-1:10, the co-degree coefficients are all more than 200, and the synergistic effect is obvious.

Application example prevention and treatment of Glycine max thrips

The insecticides prepared in the above examples and comparative examples were subjected to a field efficacy test for controlling kidney bean thrips, which was conducted in a test field in bosu county, huizhou, guangdong. The experiment is totally provided with 18 treatments and simultaneously provided with 1 blank control, each treatment is provided with 4 times of repetition, each repetition is a cell, the cells are randomly arranged, and the area of each cell is 30m²The planting habits in all test intervals were the same as the local planting habits. The number of the worm mouths is investigated 6 times before the application, the number of the survival worms is investigated 3d, 7d, 15d, 30d and 60d after the application in 17 am 6 months and 6 months in 2019, and the result is shown in table 2 by adopting a Duncan type new repolarization test method (DMRT).

TABLE 2 field control Effect of the insecticides prepared in the examples and comparative examples on thrips capsici

As can be seen from the comparison of the data of examples 1-5 and comparative examples 1-13 in Table 2, the use of steviol glycoside alone has no control effect on thrips leguminous and is combined with emamectin B_2aAfter the benzoate or the pleocidin are compounded, a certain synergistic effect is shown on the kidney bean thrips. But emamectin B_2aAfter the benzoate, the pleocidin and the stevioside are compounded and used, the inactivating effect on the pea thrips shows very obvious synergistic effect, and the synergistic effect is far superior to that of emamectin B_2aA binary combination of benzoate and spinosad. And after the spinosad is replaced by sulfoxaflor, emamectin benzoate B_2aThe binary combination of benzoate and sulfoxaflor has no synergistic effect on the peashrub and the emamectin benzoate B_2aThe ternary combination of benzoate, sulfoxaflor and stevioside also has no obvious synergistic effect on the pea thrips. After the stevioside is replaced by the astragalus polysaccharide, the control effect on the pea thrips is obviously reduced, and the emamectin B_2aControl effect of benzoate, pleocidin, stevioside and astragalus polysaccharide and emamectin benzoate B_2aThe control effect of the binary combination of benzoate and spinosad is basically equivalent, which shows that the astragalus polysaccharide has the same effect on emamectin benzoate B_2aBenzoate and spinosad have no synergistic effect.

Test results show that the ternary compound insecticidal composition has good effect of preventing and treating the kidney bean thrips, has obvious synergistic effect, prolongs the lasting period and improves the utilization rate of effective components.

The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims

1. An insecticidal composition is characterized in that the effective component of the insecticidal composition comprises emamectin benzoate B_2aBenzoate and spinosad, and the synergist comprises stevioside.

2. An insecticidal composition according to claim 1 wherein emamectin B_2aThe mass ratio of the benzoate to the spinosad to the stevioside is 1-40:1-40: 0.1-1.

3. An insecticidal composition according to claim 2 wherein emamectin B_2aThe mass ratio of the benzoate to the spinosad to the stevioside is 1-10:1-10: 0.4-0.6.

4. An insecticide comprising the insecticidal composition according to any one of claims 1 to 3.

5. The insecticide of claim 4, further comprising an adjuvant, said emamectin B_2aThe total mass of benzoate, spinosad and stevioside accounts for 2.1-81 wt% of the pesticide.

6. The insecticide of claim 5, wherein said adjuvant comprises at least one of a co-solvent, a dispersant, a wetting agent, a defoamer, a disintegrant, a thickener or a filler.

7. The insecticide according to claim 4, wherein the insecticide is in the form of microcapsule suspension, suspension seed coating, aqueous emulsion, microemulsion, emulsifiable concentrate, wettable powder, water dispersible granule or granule.

8. Use of the pesticidal composition of any one of claims 1-3 for controlling thrips pests.

9. Use of the insecticide according to any one of claims 4 to 7 for controlling thrips pests.