CN115176805B

CN115176805B - Pesticide composition containing spiromesifen and application thereof

Info

Publication number: CN115176805B
Application number: CN202210847576.6A
Authority: CN
Inventors: 张永芳; 葛家成; 刘金玲; 王均竹; 王良清
Original assignee: Qingdao Audis Biological And Technology Co ltd
Current assignee: Qingdao Audis Biological And Technology Co ltd
Priority date: 2022-07-19
Filing date: 2022-07-19
Publication date: 2023-06-23
Anticipated expiration: 2042-07-19
Also published as: CN115176805A

Abstract

The invention belongs to the technical field of pesticide compounding, and relates to a pesticide composition containing spiromesifen, which comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is spiromesifen, the active ingredient B is an isoxazoline compound, and the mass ratio of the active ingredient A to the active ingredient B is 1:25-25:1. The pesticide composition can effectively control various plant pests or mites, and has remarkable control effect.

Description

Pesticide composition containing spiromesifen and application thereof

Technical Field

The invention belongs to the technical field of pesticide compounding, and particularly relates to a pesticide composition containing spiromesifen and application thereof.

Background

Spiromesifen is a spirotetramic acid insecticidal and acaricidal agent developed by Bayer company, and has the international common name: spiromesifen, chemical name: 3- (2, 4, 6-trimethylphenyl) -2-oxo-1-oxaspiro [4,4] non-3-en-4-yl-3, 3-dimethylbutyrate. The spiromesifen has a unique spirocyclic tetronic acid structure, a novel action mechanism and excellent insecticidal and acaricidal activity, is mainly used for cotton, vegetables and ornamental plants, is used for preventing and controlling sucking mouthparts pests, and is very effective for spider mites and white flies. The action mechanism of the spiromesifen is to influence the development of whiteflies and mites, interfere the biosynthesis of liposome thereof, simultaneously can close ovarian tubes, reduce the reproductive capacity of the whiteflies and the mites and reduce the spawning quantity. The structural formula is as follows:

the chemical formula (I) is novel isoxazoline pesticide developed by Nissan chemical industry Co., ltd, the chemical name is 4- [5- (3, 5-dichlorophenyl) -4, 5-dihydro-5- (trifluoromethyl) -3-isoxazolyl-N- [ (methoxyamino) -methylene ] -2-methylbenzamide, and the S configuration is the main active ingredient. The compound shown in the formula (I) belongs to a glutamic acid-gated chloride ion channel (LGCC) interfering agent, mainly acts on gamma-aminobutyric acid-gated chloride ion channel (GABACl) of insects, is mainly used for crops such as vegetables and tea, has special effects on preventing lepidoptera pests, and can simultaneously prevent and treat acarina, thysanoptera, diptera pests or mites.

The compounds of formula (II) are non-competitive inhibitors of the gamma-aminobutyric acid (GABA) receptor by isoxazoline insecticides developed by the company n-da. The chemical name of the compound is 4- [5- (3, 5-dichloro-4-fluorophenyl) -4, 5-monohydrogen-5- (trifluoromethyl) -3-isoxazolyl ] -N- (2-ethyl-3-oxo-4-isoxazolidinyl) -2-methylbenzamide, which is a mixture of 4 active isomers, wherein the (5S, 4R) -isomer has the highest activity. The compound of the formula (II) is a broad-spectrum insecticide and acaricide, and has insecticidal activity on pests such as lepidoptera, hemiptera, coleopteran and the like.

At present, the control of field pests and mites still mainly depends on chemical control, the propagation speed of the pests or mites is high, the field application type is single, the dosage is increased, the pests and mites have different degrees of drug resistance to a plurality of medicaments, the field control effect of the medicaments is poorer and worse, and great difficulty is brought to the control. Therefore, the inventor selects medicaments with different action mechanisms for compounding, and a large number of experimental researches show that the spiromesifen and any one of the compounds in the formula (I) or the formula (II) have obvious synergistic effect on pests and mites, have good quick-acting property and long lasting time, and are safe to crops and non-target organisms.

Disclosure of Invention

Based on the above circumstances, the invention aims to provide the pesticide composition containing spiromesifen, which is mainly used for preventing and controlling plant pests or mites, especially has remarkable prevention effect on tetranychus urticae and bemisia tabaci, has the advantages of obviously enhanced pesticide effect, good quick acting property, long lasting period, safety to various plants and target organisms, effective reduction of the using amount of pesticides, reduction of production cost and reduction of environmental pollution.

In order to achieve the above purpose, the present invention adopts the following technical scheme: the pesticide composition comprises an active ingredient A and an active ingredient B, wherein the active ingredient A is spiromesifen, the active ingredient B is an isoxazoline compound, and the isoxazoline compound is any one of a compound shown in a formula (I) or a compound shown in a formula (II):

further, the mass ratio of the active ingredient A to the active ingredient B is 1:25-25:1;

further, the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:12, 1:10, 1:8, 1:7, 1:5, 1:3, 1:2, 2:3, 1:1, 3:2, 2:1, 3:1, 5:1, 7:1, 8:1, 10:1, 12:1, 15:1, 25:1;

further, the active ingredient B is a compound shown in a formula (I), and the mass ratio of the active ingredient A to the active ingredient B is 1:15-25:1;

further, the active ingredient B is a compound of the formula (I), and the mass ratio of the active ingredient A to the active ingredient B is 1:15, 1:10, 1:8, 1:5, 1:3, 1:1, 2:1, 3:1, 5:1, 8:1, 10:1, 15:1, 25:1;

further, the active ingredient B is a compound shown in a formula (I), and the mass ratio of the active ingredient A to the active ingredient B is 1:15-15:1;

further, the active ingredient B is a compound of the formula (I), and the mass ratio of the active ingredient A to the active ingredient B is 1:15, 1:10, 1:8, 1:5, 1:3, 1:1, 2:1, 3:1, 5:1, 8:1, 10:1, 15:1;

further, the active ingredient B is a compound shown in a formula (II), and the mass ratio of the active ingredient A to the active ingredient B is 1:25-15:1;

further, the active ingredient B is a compound of formula (II), and the mass ratio of the active ingredient A to the active ingredient B is 1:25, 1:15, 1:12, 1:7, 1:5, 1:2, 2:3, 1:1, 3:2, 2:1, 5:1, 7:1, 12:1, 15:1;

further, the active ingredient B is a compound shown in a formula (II), and the mass ratio of the active ingredient A to the active ingredient B is 1:12-7:1;

further, the active ingredient B is a compound of the formula (II), and the mass ratio of the active ingredient A to the active ingredient B is 1:12, 1:7, 1:5, 1:2, 2:3, 1:1, 3:2, 2:1, 5:1, 7:1;

further, the sum of the contents of the active ingredient A and the active ingredient B in the pesticide composition is 1 to 80 weight percent based on 100 weight percent of the total mass of the pesticide composition;

further, the pesticide composition comprises auxiliary components besides active components, wherein the auxiliary components are selected from one or more of wetting agents, dispersing agents, emulsifying agents, thickening agents, disintegrating agents, antifreezing agents, antifoaming agents, solvents, preservatives, stabilizers, synergists or carriers;

further, the pesticide composition comprises an agriculturally acceptable auxiliary ingredient besides an active ingredient, wherein the auxiliary ingredient is selected from one or more of wetting agents, dispersing agents, thickening agents, disintegrating agents, emulsifying agents, antifoaming agents, preservatives, stabilizers, synergists, carriers and solvents;

further, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkyl naphthalene sulfonate, lignin sulfonate, sodium dodecyl sulfate, dioctyl sodium succinate, alpha-olefin sulfonate, alkylphenol ethoxylate, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm excrement, chinese honeylocust fruit powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrating agent F; and/or

The dispersing agent is selected from one or more of lignosulfonate, alkyl naphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerin fatty acid ester polyoxyethylene ether, polycarboxylate, polyacrylic acid, phosphate, EO-PO block copolymer and EO-PO graft copolymer; and/or

The thickener is one or more selected from xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate and carboxymethyl cellulose; and/or

The disintegrating agent is one or more selected from sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and/or

The emulsifier is one or more selected from fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenethyl phenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether and fatty acid polyoxyethylene ester; and/or

The defoamer is selected from silicone oil and C ₁₀ ～C ₂₀ Saturated fatty acid compound, C ₈ ～C ₁₀ One or more of fatty alcohol compounds or silicone compounds; and/or

The preservative is one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate and methyl p-hydroxybenzoate; and/or

The stabilizer is one or more selected from disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2, 6-di-tert-butyl-p-cresol, epoxidized vegetable oil; and/or

The synergistic agent is selected from synergistic phosphorus and synergistic ether; and/or

The carrier is one or more selected from kaolin, bentonite, attapulgite, light calcium carbonate, diatomite and white carbon black; and/or

The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, N-butanol, diesel oil, N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil derivatives and deionized water; and/or

Further, the pesticide composition can be prepared into any agriculturally acceptable formulation;

further, the preparation formulation is any one of a solid preparation, a liquid preparation or a seed treatment preparation;

further, the solid preparation is a direct-use solid preparation, a dispersible solid preparation or a soluble solid preparation;

further, the directly-used solid preparation is powder, granule, sphere, tablet or strip;

the dispersible solid preparation is wettable powder, oil dispersion powder, emulsion powder, water dispersible granule, emulsion granule or water dispersible tablet;

the soluble solid preparation is soluble powder, soluble tablets or soluble granules;

further, the liquid preparation is a solution preparation, a dispersion liquid preparation, an emulsion preparation, a suspension preparation or a multiphase preparation;

further, the solution preparation is a soluble agent, an oil agent or a film spreading oil agent;

the dispersion liquid preparation is emulsifiable concentrate, emulsion, dispersible agent or paste;

the emulsion preparation is aqueous emulsion, oil emulsion, microemulsion or fat agent;

the suspension preparation is suspending agent, microcapsule suspending agent, oil suspending agent or dispersible oil suspending agent;

the multiphase preparation is a suspension emulsion, a microcapsule suspension-suspending agent, a microcapsule suspension-water emulsion or a microcapsule suspension-suspending emulsion;

further, the seed treatment preparation comprises a seed treatment solid preparation or a seed treatment liquid preparation;

further, the seed treatment solid preparation is seed treatment dry powder or seed treatment dispersible powder;

the seed treatment liquid preparation is a seed treatment liquid, a seed treatment emulsion or a seed treatment suspending agent;

further, the preparation formulation is a solid preparation and/or a liquid preparation, the liquid preparation is a suspending agent and/or emulsifiable concentrate, and the solid preparation is water dispersible granules and/or wettable powder.

The invention also discloses application of the pesticide composition in controlling plant pests and/or mites;

further, the plant pest is a Hemiptera (Hemiptera) pest, and the plant pest mites are Acarina (Acarina) pests;

further, the Hemiptera (Hemipera) pests include, but are not limited to, aphids (Aphis), scales, white flies (white flies), leafhoppers (leaf hoppers), pea aphids (Acrythosiphon pisum) (pea aphids), genus Bemisia (Adelges) and cabbage whiteflies (Aleurodes proletella), spiral white flies (Aleurodicus disperses), silk velvet flies (Aleurothrixus floccosus), genus Bemisia (Aluaca) and red scales (Aonidiella aurantii), aphids (Aphis spp.), cotton aphids (Aphis gossypii), apple aphids (Aphis pomi), potato aphids (Aulacorthum solani) (foxglove aphid), white flies (Bemisia spp.), silver leaf aphids (Bemisia argentifolii), white flies (Bemisia tabaci), wheat two-tailed aphids (apple aphid) and apple aphids (3986), cotton hoppers (24), leaf hoppers (35), leaf hoppers (35, and other than the genus Bemisia (35), the plant aphids (P., the species of the genus Erinaceus (Philaenus spp.), vitis vinifera (Phylloxera vitifoliae), leptosphaeria sequoyis (Physokermes piceae), leptosphaeria (Planococcus spp.), leptosphaeria (Pseudomonas spp.), pink (Pseudococcus brevipes), pink (Quadraspidiotus perniciosus) (Pink, san Jose scale), rhopalespeda species (Rhopalesiphum spp.), corn aphid (Rhopalosiphum maida), gramineus (Rhapalosiphum padi), leptosphaeria spp., saissima spp): eleusine (Saissetia oleracea), wheat binary aphid (Schizaphis graminum), wheat long tube aphid (Sitobion avena), white flea (Sogatella furcifera), meadow beetle species (theraaphis spp.), tortoise species (toumeella spp.), aphis species (Toxoptera spp.), meadow species (trialeude spp.), mealybugs (Trialeurodes vaporariorum), mealybugs (Trialeurodes abutiloneus), mealybugs (Unaspis spp), mealybugs (Unaspis yanonensis).

Further, the hemiptera pest is a sternoclavia pest, and the sternoclavia pest is an Aleyrodidae pest;

further, the whitefly pest is bemisia tabaci (Bemisia tabaci Gennadius).

Further, the acarina pest mites comprise Tetranychioideae (Tetranychoidae) pest mites, eriophtalmidae (Eriophyoidea) pest mites and Tarsonemidae (Tarsonemoidea) pest mites;

further, the pest mites are tetranychoidae (tetranychoidae);

further, the tetranychus urticae comprises any one or more of tetranychus urticae (Tetranychus urticae), tetranychus cinnabarinus (Tetranychus cinnabarinus), panonychus citri (pannychus citri), panonychus ulmi (pannychus ulmi) and tetranychus citri (Eotetranychus kankitus);

further, the tetranychidae mite is tetranychidae (Tetranychus urticae);

further, the pesticidal composition and/or the formulation thereof is applied to the pest and/or the pest mites or the medium in which they grow in an effective amount.

Compared with the prior art, the invention has the following beneficial effects:

1) The composition of the invention is prepared by compounding compounds with different action mechanisms, and can obviously enhance the drug effect;

2) The pesticide consumption is reduced, the production cost is reduced, the residue of the pesticide on crops is reduced, the safety and environmental protection are realized, and the environmental pollution is reduced;

3) The composition of the invention can effectively slow down the generation of drug resistance of pests or mites.

Detailed Description

The present invention will be described in more detail with reference to the following examples, but the present invention can be embodied in various forms and should not be construed as being limited to the embodiments set forth herein.

Preparation examples of related formulations:

preparation example 1:20% spiromesifen-Compound suspending agent of formula (I) (1:3)

5% spiromesifen, 15% of a compound shown in a formula (I), 1% of succinate sulfonate, 1% of naphthalene sulfonate formaldehyde condensate, 3% of styrol polyoxyethylene ether phosphate, 0.2% of xanthan gum, 1% of magnesium aluminum silicate, 4% of propylene glycol, 1% of sodium sorbate, 0.5% of silicone oil and deionized water, wherein the balance is the balance;

the preparation method comprises the following steps: according to the formula proportion, the effective components spiromesifen, a compound shown in a formula (I), a surfactant and other functional auxiliary agents are sequentially placed in a reaction kettle, water is added and mixed uniformly, high-speed shearing and wet sanding are carried out, and finally, the suspending agent product is obtained through homogenizing and filtering.

Preparation example 2:15% spiromesifen-Compound emulsifiable concentrate of formula (I) (1:1)

The weight percentage of the spiromesifen is 7.5 percent, the weight percentage of the compound of the formula (I) is 7.5 percent, the weight percentage of the propylene glycol methyl ether is 15 percent, the weight percentage of the styrylphenol polyoxyethylene ether is 13 percent, the weight percentage of the calcium dodecyl benzene sulfonate is 2 percent, the weight percentage of the propylene carbonate is 15 percent, and the rest is the xylene.

The preparation method comprises the following steps: adding the measured spiromesifen, the compound of the formula (I), the solvent and the cosolvent into a blending kettle, stirring to dissolve the spiromesifen, adding the emulsifier, supplementing the rest with the residual solvent, stirring uniformly in the stirring kettle, and filtering to obtain the emulsifiable concentrate required by the invention.

Preparation example 3:21% spiromesifen-Compound water dispersible granule of formula (I) (2:1)

14% of spiromesifen, 7% of a compound shown in a formula (I), 10% of sodium lignin sulfonate, 4% of sodium dodecyl benzene sulfonate, 2% of nekal BX, 5% of white carbon black, 10% of ammonium sulfate and the balance of kaolin.

The preparation method comprises the following steps: according to the formula proportion of the embodiment, adding the spiromesifen as an active ingredient and a compound shown in a formula (I) into a carrier, adding a surfactant and other functional auxiliary agents into the carrier, mixing, adding 10-25% of water after jet milling, and then kneading, granulating, drying and screening to prepare a water dispersible granule product; or spraying water, granulating, drying, and sieving to obtain the final product.

Preparation example 4:21% spiromesifen-Compound suspending agent of formula (II) (1:2)

The weight percentage of the components is 7 percent of spiromesifen, 14 percent of the compound of the formula (II), 0.5 percent of alkylphenol ethoxylate, 4 percent of alkylphenol ethoxylate phosphate, 1 percent of sodium lignin sulfonate, 0.25 percent of xanthan gum, 5 percent of glycerol, 0.1 percent of sodium benzoate, 0.5 percent of silicone oil and the balance of deionized water.

The preparation method comprises the following steps: the same as in preparation example 1.

Preparation example 5:18% spiromesifen-Compound emulsifiable concentrate of formula (II) (1:1)

9% spiromesifen, 9% of a compound of formula (II), 20% of EO/PO block copolymer, 10% of dimethyl sulfoxide, 10% of DMF, 2% of calcium dodecyl benzene sulfonate and the balance of methyl oleate.

The preparation method comprises the following steps: the same as in preparation example 2.

Preparation example 6:25% spiromesifen-Compound wettable powder of formula (II) (2:3)

10% of spiromesifen, 15% of a compound shown in a formula (II), 4% of sodium lignin sulfonate, 4% of naphthalene sulfonate formaldehyde condensate, 2% of nekal BX, 5% of white carbon black and kaolin, and the balance of the composition;

the preparation method comprises the following steps: the effective components, the dispersing agent, the wetting agent and the filler are mixed according to the formula proportion, evenly stirred in a stirring kettle, and crushed and mixed evenly for many times by an airflow crusher, thus obtaining the wettable powder of the composition.

Indoor activity test:

example 1: indoor combined action test of mixed medicament on two-spotted spider mites

The test is based on: test reference NY/T1154.12-2008 "pesticide indoor bioassay test guidelines section 12: tetranychus slide impregnation method, NY/T1154.7-2008 pesticide section 7, guidelines for pesticide indoor bioassay test: determination of the combined action of compounding.

Test agent: 92% of the raw medicine of the compound of the formula (I), 98% of the raw medicine of the compound of the formula (II) and 98% of the raw medicine of the spiromesifen are provided by a research and development center of the sea li pharmaceutical industry group.

Test target: tetranychus urticae (Tetranychus urticae Koch), female adult mites with consistent physiological states are selected for indoor feeding.

Preparing a test material: cutting the double-sided adhesive tape into 2cm multiplied by 2cm, attaching the double-sided adhesive tape onto one section of a glass slide, selecting healthy female adult mites with consistent physiological state, picking up the female adult mites with a writing brush to enable the backs of the female adult mites to be lightly adhered to the adhesive tape downwards, putting each glass slide 30 mites into a pest-culturing dish filled with wet sponge, covering a cover, placing the glass slide in the condition of (25+/-1) DEG C, performing microscopic examination after 2 hours, removing dead and injured individuals, and supplementing each glass slide 30 mites.

And (3) preparation of a medicament: the mother solution of single agent is prepared respectively, and the required series of mass concentrations of each single agent and each group of mixed agent are prepared according to the method of equal ratio according to the mixing purpose and the reasonable mixing ratio of the medicament activity design.

And (3) medicament treatment: immersing the glass slide in the liquid medicine, slightly shaking for 5s, taking out, sucking the excessive liquid medicine by using water absorbing paper, placing the glass slide in a white porcelain plate filled with sponge, and covering the glass slide with a plastic film with good light transmittance. Each treatment was repeated 4 times and a treatment without the agent (with all organic solvents and emulsifiers) was set as a blank.

And (3) feeding and observing: and (3) placing the insect culture dish with the treated test insects at the temperature of (25+/-1) DEG C and the photoperiod of L:D= (16:8) h for feeding and observing.

Data statistics and analysis: and (5) checking death conditions of the test insects 48h after treatment, and recording total insects and death insects respectively.

From the survey data, corrected mortality for each treatment was calculated. Calculating according to formulas (1) and (2), wherein the calculation results are reserved to two positions after decimal point:

wherein:

p-mortality in percent (%);

k-represents the number of dead insects in units of heads;

n-represents the total number of insects treated in units of heads.

Wherein:

P ₁ -correct mortality in percent (%);

P _t mortality rate in percent (%);

P ₀ blank mortality in percent (%).

If the control mortality is less than 5%, correction is not needed; the control mortality is between 5% and 20%, and correction is carried out according to the formula (2); control mortality was > 20% and the test was reworked.

Analyzing with IBM SPSS Statistics statistical analysis system to obtain virulence regression equation and LC ₅₀ Value and correlation coefficient R ² The activity of the test agent on the biological test material was evaluated.

The co-toxicity coefficient (CTC value) of the mixture is calculated according to the formulas (3), (4) and (5):

wherein:

ati—actual measured virulence index of the mixture;

S-LC of Standard acaricide ₅₀ Milligrams per liter (mg/L);

M-LC of the mixture ₅₀ Units are milligrams per liter (mg/L).

TTI＝TI _A ^* P _A +TI _B ^* P _B ·······(4)

Wherein:

TTI-the theoretical toxicity index of the mixture;

TI _A -a medicament virulence index;

P _A -the percentage of agent a in the mix, in percent (%);

TI _B -B agent virulence index;

P _B the percentage of the B medicament in the mixture is expressed as percentage (%).

Wherein:

ctc—co-toxicity coefficient;

ati—actual measured virulence index of the mixture;

TTI-the theoretical toxicity index of the mixture.

The compound co-toxicity coefficient CTC is more than or equal to 120 and shows synergistic effect; ctc.ltoreq.80 shows antagonism; 80 < CTC < 120 shows additive effect.

The results of the indoor toxicity test are shown in the following table:

TABLE 1 indoor combined action test of spiromesifen and Compound of formula (I) against Tetranychus urticae

TABLE 2 indoor combined action test of spiromesifen and Compound of formula (II) against Tetranychus urticae

As shown by indoor activity tests, spiromesifen has higher toxicity to Tetranychus urticae, and LC thereof ₅₀ The mass ratio of spiromesifen to the compound of formula (I) is 1:15 to the extent of 1.317mg/LWithin the range of 25:1, the combined effect is manifested as a synergistic effect; the mass ratio of spiromesifen to the compound in the formula (I) is in the range of 1:10-5:1, the co-toxicity coefficient is more than 130, and the synergy is remarkable; the mass ratio of spiromesifen to the compound in the formula (I) is 1:3, and the co-toxicity coefficient is the largest and is 154.723.

The mass ratio of spiromesifen to the compound in the formula (II) is in the range of 1:15-15:1, the co-toxicity coefficient is more than 120, and the combined action shows a synergistic effect; the mass ratio of spiromesifen to the compound in the formula (II) is in the range of 1:12-7:1, the co-toxicity coefficient is more than 130, and the synergy is remarkable; the mass ratio of spiromesifen to the compound of the formula (II) is 1:2, and the co-toxicity coefficient is the largest and is 161.414.

Example 2: indoor combined action test of mixed medicament on bemisia tabaci

The test is based on: test reference NY/T1154.9-2008 "pesticide in laboratory bioassay test guidelines section 9: spray method, NY/T1154.7-2008 pesticide section 7, guidelines for indoor bioassay tests for pesticides: determination of the combined action of compounding.

Test target: bemisia tabaci (Bemisia tabaci Gennadius) is selected for indoor feeding, and two-age nymphs with consistent physiological states.

Potter spray tower preparation: stabilizing the spray pressure of the Potter spray tower at 1.47×10 ⁵ Pa, the spray head was first rinsed 2 times with acetone and then 2 times with distilled water.

And (3) medicament treatment: firstly, 20 insects with consistent physiological states are selected by a writing brush and put into a culture dish, then the culture dish is put into a bottom plate of a Potter spray tower for quantitative spraying, the spraying dosage is 1mL, and after the medicines settle for 1min, the insects are taken out for breeding. Each treatment was repeated 4 times and a treatment without the agent (with all organic solvents and emulsifiers) was set as a blank.

Feeding and observing: the treated insects are fed and observed under the conditions of the temperature of (25+/-1), the relative humidity of (70+/-5) percent and the photoperiod of L: D= (16:8) h.

wherein:

p-mortality in percent (%);

k-represents the number of dead insects in units of heads;

n-represents the total number of insects treated in units of heads.

Wherein:

P ₁ -correct mortality in percent (%);

P _t mortality rate in percent (%);

P ₀ blank mortality in percent (%).

wherein:

ati—actual measured virulence index of the mixture;

S-LC of Standard insecticide ₅₀ Milligrams per liter (mg/L);

M-LC of the mixture ₅₀ Units are milligrams per liter (mg/L).

TTI＝TI _A *P _A +TI _B *P _B ·······(4)

Wherein:

TTI-the theoretical toxicity index of the mixture;

TI _A -a medicament virulence index;

P _A -the percentage of agent a in the mix, in percent (%);

TI _B -B agent virulence index;

Wherein:

ctc—co-toxicity coefficient;

ati—actual measured virulence index of the mixture;

TTI-the theoretical toxicity index of the mixture.

The results of the indoor toxicity test are shown in the following table:

TABLE 3 indoor combination test of spiromesifen and Compound of formula (I) against Bemisia tabaci

TABLE 4 indoor combination test of spiromesifen and Compound of formula (II) against Bemisia tabaci

Test agent	Toxicity regression equation	Correlation coefficient R ²	LC ₅₀ (mg/L)	Co-toxicity coefficient
					Spiromesifen (A)	y＝2.061x-2.244	0.998	12.260	/
Compounds of formula (II) (B)	y＝2.425x-2.362	0.993	9.418	/
					A:B＝1:25	y＝1.888x-1.666	0.992	7.624	124.642
A:B＝1:15	y＝1.718x-1.410	0.992	6.621	144.336
					A:B＝1:7	y＝1.837x-1.477	0.991	6.367	152.333
A:B＝2:3	y＝1.935x-1.492	0.990	5.906	175.762
					A:B＝1:1	y＝1.571x-1.240	0.993	6.158	172.990
A:B＝3:2	y＝2.158x-1.802	0.993	6.844	159.841
					A:B＝7:1	y＝1.956x-1.739	0.990	7.741	152.621
A:B＝15:1	y＝1.775x-1.673	0.990	8.759	137.379
					A:B＝25:1	y＝1.873x-1.816	0.997	9.324	129.980

According to indoor activity tests, the mass ratio of spiromesifen to the compound of the formula (I) is in the range of 1:15-15:1, and the combined action shows a synergistic effect; the mass ratio of spiromesifen to the compound in the formula (I) is in the range of 1:8-8:1, the co-toxicity coefficient is more than 130, and the synergy is remarkable; the mass ratio of spiromesifen to the compound in the formula (I) is 2:1, and the co-toxicity coefficient is the largest and is 178.633.

The mass ratio of spiromesifen to the compound in the formula (II) is in the range of 1:25-25:1, the co-toxicity coefficient is more than 120, and the combined action shows a synergistic effect; the mass ratio of spiromesifen to the compound in the formula (II) is in the range of 1:15-7:1, the co-toxicity coefficient is more than 140, and the synergy is remarkable; the mass ratio of spiromesifen to the compound of formula (II) is 2:3, and the co-toxicity coefficient is the largest and is 175.762.

Field efficacy test

Example 3: field efficacy test of mixed medicament on two-spotted spider mites

The test is based on: the test refers to GB/T17980.17-2000 "pesticide field efficacy test criterion (one) acaricide for controlling beans and vegetable spider mites".

Test site: the soil fertility of the test land is higher in Shao Guzhuang village of baoding city in Hebei province, the cultivation and management are good, the strawberry in each district of the test land is planted in the same period, the water and fertilizer management is consistent, and the growth vigor is even.

Test crop: strawberry (red color).

Test target: tetranychus urticae (Tetranychus urticae Koch).

Cell arrangement: the cells are arranged in random groups, and each cell has an area of 30m ² Each treatment was repeated 4 times, with two rows of strawberries per cell interval.

The application method comprises the following steps: the test is carried out on the 5 th and 20 th days of 2019, the water is added according to the designed dosage of the test for 1 time, the liquid medicine is evenly sprayed on the front and the back of the blade, and the liquid medicine amount is 675kg/hm ² 。

The investigation method comprises the following steps: the number of mites was investigated before administration, and the number of live mites was investigated 1d, 3d and 7d after administration. And (5) taking 20 leaves for statistics and activation of mites in each cell.

The drug effect calculation method comprises the following steps:

during the test period, the strawberries in all the treatment cells are observed to grow well, and all the treatment medicaments are not harmful to strawberry plants and other non-target organisms under the tested concentration.

The field efficacy test is shown in the following table:

table 5 results of field efficacy test of the Mixed Agents against Tetranychus urticae

As shown by the field efficacy test results, spiromesifen and any one of the compounds in the formula (I) or the formula (II) show good control effect on the tetranychus urticae. After 1d of the medicine, the control effect of each compound preparation on the tetranychus urticae is more than 72.55%, and each preparation example shows good quick-acting performance.

After the medicine is taken, the control effects of the 7d,15% spiromesifen-formula (I) compound emulsifiable concentrate (1:1), 18% spiromesifen-formula (II) compound emulsifiable concentrate (1:1), 20% spiromesifen-formula (I) compound suspending agent (1:3) and 21% spiromesifen-formula (II) compound suspending agent (1:2) are 82.46%, 84.08%, 85.91% and 87.82% respectively, and the better lasting effect is shown.

Example 4: field efficacy test of mixed medicament on tomato bemisia tabaci

The test is based on: test reference NY/T1464.43-2012 pesticide field efficacy test guidelines part 43: the pesticide can prevent and treat vegetable bemisia tabaci.

Test site: in vegetable greenhouse in Qingdao city, shandong province, the soil of the test land is sandy loam, and the organic matter content is about 1%. All the test cells are uniform in cultivation condition and accord with local agricultural specifications.

Test crop: tomato (ai Feng).

Test target: bemisia tabaci (Bemisia tabaci).

Cell arrangement: area per cell 20m ² Each treatment was repeated 4 times.

The application method comprises the following steps: the first pesticide application is started when 3-5 times of adults are reached in each plant, the test is carried out in 7 months in 2020, and the industrial pesticide-16 type knapsack sprayer is used for diluting the test pesticide according to the designed proportion, and the whole plant spraying is carried out on tomato plants, so that the front and back surfaces of the leaves are uniformly coated with the pesticide.

The investigation method comprises the following steps: 10 plants of crops are calibrated in each district, whole plants of adults are investigated in the morning when the adults are inactive, and the number of living adults is recorded. The number of insect population was examined before the application, and the number of insect population on the strain was examined at 1d, 3d and 7d after the application.

The drug effect calculation method comprises the following steps:

during the test period, the treated tomatoes in each district are observed to grow well, the tomato plants have no phytotoxicity symptoms such as leaf discoloration, wilting, deformity and the like, the tomato fruits grow normally, and the treated medicaments have no phytotoxicity to the tomato plants and other non-target organisms under the tested concentration.

The field efficacy test is shown in the following table:

table 6 field efficacy test of the compounded formulation against tomato bemisia tabaci

As can be seen from the field efficacy test results, the pesticide composition obtained by compounding spiromesifen with any one of the compound of the formula (I) or the compound of the formula (II) has better control effect on bemisia tabaci and good quick-acting property.

The pesticide composition and/or the preparation thereof provided by the invention have the advantages that the pesticide composition and/or the preparation thereof has a good control effect on pests and mites, the pesticide composition has good quick-acting property and long lasting period, and the pesticide composition is safe to crops without drug injury to crops caused by the compound medicament in the test.

The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement the same, and are not intended to limit the scope of the present invention. All equivalent changes or modifications made in accordance with the spirit of the present invention are intended to be included within the scope of the present invention.

Claims

1. The pesticide composition containing spiromesifen is characterized by comprising an active ingredient A and an active ingredient B, wherein the active ingredient A is spiromesifen, the active ingredient B is an isoxazoline compound, and the isoxazoline compound is any one of compounds shown in a formula (I) or a formula (II):

、

the active ingredient B is a compound shown in a formula (I), and the mass ratio of the active ingredient A to the active ingredient B is 1:15-25:1; the active ingredient B is a compound shown in a formula (II), and the mass ratio of the active ingredient A to the active ingredient B is 1:25-15:1.

2. The pesticide composition as set forth in claim 1, wherein the active ingredient B is a compound of formula (I), and the mass ratio of the active ingredient a to the active ingredient B is 1:15 to 15:1; the active ingredient B is a compound shown in a formula (II), and the mass ratio of the active ingredient A to the active ingredient B is 1:12-7:1.

3. A pesticide composition as set forth in claim 1, characterized in that the sum of the contents of said active ingredient a and active ingredient B in the pesticide composition is 1 to 80% by weight based on 100% by weight of the total mass of the pesticide composition.

4. A pesticide composition as set forth in claim 1, wherein said pesticide composition comprises, in addition to the active ingredient, an auxiliary ingredient selected from one or more of a wetting agent, a dispersing agent, an emulsifying agent, a thickening agent, a disintegrating agent, an antifreezing agent, an antifoaming agent, a solvent, a preservative, a stabilizer, a synergist or a carrier.

5. A pesticide composition as set forth in claim 1, wherein said pesticide composition is capable of being formulated into any one of agriculturally acceptable dosage forms, said dosage forms being solid, liquid;

the solid preparation comprises powder, granules, balls, tablets, strips, wettable powder, oil dispersion powder, emulsion powder, water dispersible granules, emulsion granules, water dispersible tablets, soluble powder, soluble tablets or soluble granules;

the liquid preparation comprises a soluble agent, an oil agent, a spreading oil agent, an emulsifiable concentrate, emulsion, dispersible agent, ointment, aqueous emulsion, oil emulsion, microemulsion, lipid suspending agent, microcapsule suspending agent, oil suspending agent, dispersible oil suspending agent, suspending emulsion, microcapsule suspending-suspending agent, microcapsule suspending-aqueous emulsion or microcapsule suspending-suspending emulsion.

6. A pesticidal composition according to claim 5, wherein the formulation is a solid and/or a liquid formulation; the liquid preparation is suspending agent and/or emulsifiable solution, and the solid preparation is water dispersible granule and/or wettable powder.

7. Use of a pesticidal composition according to any one of claims 1 to 6 for controlling plant pests and/or mites.

8. The use according to claim 7, wherein the plant pest is a Hemiptera (Hemiptera) pest and the plant pest mite is a Acarina (acrina) pest.

9. The use according to claim 8, wherein the hemipteran pest is a subthoracic (sternorrhanchach) pest, and the subthoracic pest is a Aleyrodidae (Aleyrodidae) pest;

the Acarina pest mites are Tetranychioidea pest mites of Tetranychidae (Eriophoidea) pest mites of Tarsonemidae (Tarsonemoidea).

10. The use according to claim 7, wherein the pesticidal composition and/or formulation thereof is applied to the pest and/or pest mites or the medium in which they are growing in an effective amount.